Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031473.01.T01 | XP_004489604.1 | 77.049 | 183 | 33 | 1 | 1 | 183 | 1 | 174 | 1.13E-84 | 257 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031473.01.T01 | O82081 | 38.194 | 144 | 85 | 3 | 4 | 144 | 5 | 147 | 1.53E-29 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031473.01.T01 | A0A1S2XHV2 | 77.049 | 183 | 33 | 1 | 1 | 183 | 1 | 174 | 5.41e-85 | 257 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024980.01 | MsG0680031473.01 | 0.832301 | 1.022150e-55 | 1.137696e-52 |
| MsG0380017975.01 | MsG0680031473.01 | 0.806870 | 6.668094e-50 | 3.659653e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380017027.01 | MsG0680031473.01 | PPI |
| MsG0680035247.01 | MsG0680031473.01 | PPI |
| MsG0680031473.01 | MsG0680035054.01 | PPI |
| MsG0780039353.01 | MsG0680031473.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031473.01.T01 | MTR_6g023760 | 87.500 | 184 | 17 | 2 | 1 | 183 | 1 | 179 | 3.10e-88 | 256 |
| MsG0680031473.01.T01 | MTR_2g088990 | 43.697 | 119 | 65 | 2 | 1 | 118 | 1 | 118 | 3.76e-31 | 113 |
| MsG0680031473.01.T01 | MTR_6g080660 | 38.596 | 114 | 69 | 1 | 7 | 120 | 10 | 122 | 4.73e-30 | 109 |
| MsG0680031473.01.T01 | MTR_5g006040 | 36.478 | 159 | 91 | 5 | 4 | 159 | 6 | 157 | 4.88e-27 | 101 |
| MsG0680031473.01.T01 | MTR_8g007020 | 40.127 | 157 | 86 | 4 | 8 | 159 | 9 | 162 | 4.92e-27 | 101 |
| MsG0680031473.01.T01 | MTR_3g099570 | 32.584 | 178 | 119 | 1 | 6 | 183 | 7 | 183 | 4.15e-25 | 96.3 |
| MsG0680031473.01.T01 | MTR_8g007035 | 46.087 | 115 | 57 | 3 | 8 | 120 | 9 | 120 | 4.59e-25 | 96.7 |
| MsG0680031473.01.T01 | MTR_2g025580 | 36.752 | 117 | 71 | 2 | 7 | 120 | 7 | 123 | 1.39e-24 | 94.7 |
| MsG0680031473.01.T01 | MTR_3g099580 | 40.870 | 115 | 67 | 1 | 6 | 120 | 7 | 120 | 1.14e-23 | 92.4 |
| MsG0680031473.01.T01 | MTR_1g090190 | 28.571 | 161 | 114 | 1 | 22 | 182 | 20 | 179 | 6.69e-22 | 87.8 |
| MsG0680031473.01.T01 | MTR_3g099540 | 38.655 | 119 | 69 | 2 | 8 | 126 | 13 | 127 | 3.84e-21 | 84.7 |
| MsG0680031473.01.T01 | MTR_1g104800 | 31.410 | 156 | 102 | 4 | 22 | 174 | 22 | 175 | 1.20e-20 | 84.7 |
| MsG0680031473.01.T01 | MTR_1g105130 | 34.483 | 116 | 73 | 3 | 7 | 120 | 7 | 121 | 1.53e-20 | 83.6 |
| MsG0680031473.01.T01 | MTR_1g105120 | 34.483 | 116 | 73 | 3 | 7 | 120 | 7 | 121 | 1.53e-20 | 83.6 |
| MsG0680031473.01.T01 | MTR_7g086220 | 31.967 | 122 | 80 | 3 | 1 | 120 | 1 | 121 | 1.07e-19 | 81.6 |
| MsG0680031473.01.T01 | MTR_2g101300 | 34.641 | 153 | 89 | 6 | 6 | 153 | 10 | 156 | 2.28e-19 | 81.6 |
| MsG0680031473.01.T01 | MTR_4g007250 | 35.354 | 99 | 63 | 1 | 22 | 120 | 26 | 123 | 3.41e-19 | 80.5 |
| MsG0680031473.01.T01 | MTR_3g099980 | 36.441 | 118 | 74 | 1 | 4 | 120 | 5 | 122 | 3.66e-19 | 81.6 |
| MsG0680031473.01.T01 | MTR_8g086360 | 30.556 | 180 | 114 | 5 | 8 | 176 | 5 | 184 | 4.49e-19 | 80.9 |
| MsG0680031473.01.T01 | MTR_3g099680 | 35.833 | 120 | 76 | 1 | 4 | 122 | 5 | 124 | 5.37e-19 | 81.3 |
| MsG0680031473.01.T01 | MTR_4g114870 | 34.815 | 135 | 84 | 1 | 6 | 136 | 10 | 144 | 7.00e-19 | 83.2 |
| MsG0680031473.01.T01 | MTR_4g114870 | 37.624 | 101 | 57 | 3 | 25 | 120 | 191 | 290 | 5.25e-17 | 77.8 |
| MsG0680031473.01.T01 | MTR_0334s0010 | 35.593 | 118 | 72 | 3 | 6 | 120 | 9 | 125 | 5.33e-18 | 78.2 |
| MsG0680031473.01.T01 | MTR_4g111640 | 32.090 | 134 | 83 | 3 | 23 | 150 | 30 | 161 | 1.21e-17 | 76.6 |
| MsG0680031473.01.T01 | MTR_4g077787 | 30.935 | 139 | 86 | 3 | 23 | 155 | 30 | 164 | 2.92e-17 | 75.9 |
| MsG0680031473.01.T01 | MTR_7g086140 | 27.222 | 180 | 106 | 4 | 4 | 181 | 5 | 161 | 4.16e-17 | 74.7 |
| MsG0680031473.01.T01 | MTR_7g086190 | 27.222 | 180 | 106 | 4 | 4 | 181 | 5 | 161 | 4.16e-17 | 74.7 |
| MsG0680031473.01.T01 | MTR_8g088830 | 43.689 | 103 | 48 | 6 | 20 | 118 | 23 | 119 | 4.19e-17 | 73.9 |
| MsG0680031473.01.T01 | MTR_4g067200 | 31.724 | 145 | 76 | 5 | 6 | 143 | 9 | 137 | 1.48e-16 | 73.6 |
| MsG0680031473.01.T01 | MTR_4g066110 | 31.724 | 145 | 76 | 5 | 6 | 143 | 9 | 137 | 1.48e-16 | 73.6 |
| MsG0680031473.01.T01 | MTR_2g043730 | 36.207 | 116 | 69 | 3 | 6 | 119 | 11 | 123 | 1.83e-16 | 72.0 |
| MsG0680031473.01.T01 | MTR_3g089035 | 40.816 | 98 | 53 | 4 | 22 | 118 | 30 | 123 | 2.25e-16 | 72.0 |
| MsG0680031473.01.T01 | MTR_3g105930 | 36.232 | 138 | 78 | 6 | 22 | 150 | 24 | 160 | 2.32e-16 | 73.2 |
| MsG0680031473.01.T01 | MTR_3g089005 | 37.069 | 116 | 67 | 5 | 6 | 119 | 13 | 124 | 3.00e-16 | 71.6 |
| MsG0680031473.01.T01 | MTR_1g112700 | 31.293 | 147 | 93 | 5 | 9 | 149 | 17 | 161 | 4.42e-16 | 72.8 |
| MsG0680031473.01.T01 | MTR_7g086100 | 29.060 | 117 | 83 | 0 | 4 | 120 | 5 | 121 | 4.80e-16 | 73.6 |
| MsG0680031473.01.T01 | MTR_2g090580 | 27.225 | 191 | 122 | 3 | 6 | 179 | 8 | 198 | 5.09e-16 | 72.8 |
| MsG0680031473.01.T01 | MTR_7g086090 | 31.405 | 121 | 82 | 1 | 1 | 120 | 1 | 121 | 6.67e-16 | 73.2 |
| MsG0680031473.01.T01 | MTR_8g463180 | 31.973 | 147 | 85 | 5 | 1 | 144 | 1 | 135 | 6.75e-16 | 74.3 |
| MsG0680031473.01.T01 | MTR_3g117110 | 31.410 | 156 | 99 | 6 | 1 | 150 | 3 | 156 | 1.36e-15 | 70.9 |
| MsG0680031473.01.T01 | MTR_5g088000 | 29.787 | 141 | 92 | 4 | 6 | 143 | 1 | 137 | 1.47e-15 | 70.1 |
| MsG0680031473.01.T01 | MTR_8g094990 | 31.356 | 118 | 76 | 2 | 8 | 120 | 6 | 123 | 2.53e-15 | 72.4 |
| MsG0680031473.01.T01 | MTR_2g090575 | 33.582 | 134 | 81 | 5 | 6 | 133 | 7 | 138 | 3.28e-15 | 72.8 |
| MsG0680031473.01.T01 | MTR_8g095013 | 30.508 | 118 | 77 | 2 | 8 | 120 | 6 | 123 | 3.45e-15 | 72.0 |
| MsG0680031473.01.T01 | MTR_6g013170 | 35.246 | 122 | 74 | 3 | 25 | 145 | 31 | 148 | 4.20e-15 | 69.7 |
| MsG0680031473.01.T01 | MTR_7g086160 | 28.659 | 164 | 92 | 4 | 20 | 181 | 22 | 162 | 4.71e-15 | 69.3 |
| MsG0680031473.01.T01 | MTR_6g013420 | 29.655 | 145 | 88 | 5 | 6 | 146 | 17 | 151 | 5.36e-15 | 69.7 |
| MsG0680031473.01.T01 | MTR_4g112310 | 32.143 | 112 | 74 | 2 | 39 | 150 | 2 | 111 | 5.59e-15 | 68.6 |
| MsG0680031473.01.T01 | MTR_1g014120 | 33.333 | 141 | 82 | 6 | 6 | 139 | 11 | 146 | 5.76e-15 | 69.7 |
| MsG0680031473.01.T01 | MTR_6g022170 | 34.711 | 121 | 70 | 5 | 20 | 133 | 29 | 147 | 1.87e-14 | 67.8 |
| MsG0680031473.01.T01 | MTR_4g078410 | 27.389 | 157 | 107 | 3 | 22 | 171 | 22 | 178 | 5.46e-14 | 67.0 |
| MsG0680031473.01.T01 | MTR_7g086230 | 28.926 | 121 | 85 | 1 | 1 | 120 | 1 | 121 | 6.31e-14 | 66.6 |
| MsG0680031473.01.T01 | MTR_7g086280 | 28.926 | 121 | 85 | 1 | 1 | 120 | 1 | 121 | 6.71e-14 | 66.6 |
| MsG0680031473.01.T01 | MTR_8g099220 | 37.864 | 103 | 59 | 4 | 22 | 120 | 26 | 127 | 1.73e-13 | 64.3 |
| MsG0680031473.01.T01 | MTR_2g043790 | 36.364 | 99 | 60 | 2 | 21 | 119 | 3 | 98 | 2.33e-13 | 63.5 |
| MsG0680031473.01.T01 | MTR_4g081100 | 34.694 | 98 | 60 | 1 | 27 | 120 | 34 | 131 | 3.26e-13 | 65.9 |
| MsG0680031473.01.T01 | MTR_2g083250 | 35.000 | 100 | 59 | 3 | 27 | 120 | 28 | 127 | 3.28e-13 | 66.2 |
| MsG0680031473.01.T01 | MTR_2g079030 | 27.545 | 167 | 117 | 3 | 4 | 168 | 5 | 169 | 7.18e-13 | 66.2 |
| MsG0680031473.01.T01 | MTR_1g077790 | 33.036 | 112 | 68 | 4 | 16 | 121 | 20 | 130 | 1.43e-12 | 63.5 |
| MsG0680031473.01.T01 | MTR_4g124280 | 29.839 | 124 | 82 | 3 | 1 | 120 | 2 | 124 | 7.67e-12 | 62.4 |
| MsG0680031473.01.T01 | MTR_5g088170 | 27.607 | 163 | 91 | 7 | 22 | 180 | 13 | 152 | 2.50e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031473.01.T01 | AT3G27200 | 63.478 | 115 | 42 | 0 | 6 | 120 | 7 | 121 | 6.26e-52 | 164 |
| MsG0680031473.01.T01 | AT5G07475 | 38.065 | 155 | 90 | 4 | 11 | 164 | 16 | 165 | 6.75e-31 | 111 |
| MsG0680031473.01.T01 | AT2G32300 | 38.194 | 144 | 85 | 3 | 4 | 144 | 5 | 147 | 1.56e-30 | 112 |
| MsG0680031473.01.T01 | AT2G32300 | 38.194 | 144 | 85 | 3 | 4 | 144 | 35 | 177 | 4.78e-30 | 111 |
| MsG0680031473.01.T01 | AT2G26720 | 31.472 | 197 | 116 | 5 | 5 | 183 | 7 | 202 | 2.41e-25 | 97.4 |
| MsG0680031473.01.T01 | AT2G31050 | 43.860 | 114 | 61 | 3 | 9 | 120 | 13 | 125 | 1.87e-24 | 95.1 |
| MsG0680031473.01.T01 | AT1G72230 | 30.899 | 178 | 121 | 2 | 5 | 182 | 3 | 178 | 2.90e-20 | 83.6 |
| MsG0680031473.01.T01 | AT4G31840 | 33.889 | 180 | 101 | 7 | 7 | 180 | 10 | 177 | 3.72e-20 | 83.2 |
| MsG0680031473.01.T01 | AT5G26330 | 32.778 | 180 | 110 | 5 | 7 | 176 | 4 | 182 | 7.47e-20 | 82.8 |
| MsG0680031473.01.T01 | AT2G27035 | 27.976 | 168 | 105 | 6 | 6 | 170 | 9 | 163 | 1.99e-19 | 80.9 |
| MsG0680031473.01.T01 | AT3G18590 | 40.323 | 124 | 65 | 5 | 4 | 120 | 5 | 126 | 9.63e-19 | 79.7 |
| MsG0680031473.01.T01 | AT1G48940 | 37.209 | 129 | 68 | 5 | 1 | 120 | 1 | 125 | 1.36e-18 | 79.3 |
| MsG0680031473.01.T01 | AT3G60270 | 40.594 | 101 | 59 | 1 | 20 | 120 | 21 | 120 | 1.39e-18 | 79.3 |
| MsG0680031473.01.T01 | AT5G53870 | 32.317 | 164 | 106 | 4 | 3 | 163 | 9 | 170 | 2.41e-18 | 81.6 |
| MsG0680031473.01.T01 | AT5G53870 | 32.317 | 164 | 106 | 4 | 3 | 163 | 9 | 170 | 4.18e-18 | 80.9 |
| MsG0680031473.01.T01 | AT1G45063 | 37.963 | 108 | 62 | 4 | 19 | 121 | 141 | 248 | 7.21e-18 | 79.3 |
| MsG0680031473.01.T01 | AT1G45063 | 31.304 | 115 | 77 | 2 | 8 | 120 | 12 | 126 | 1.68e-11 | 61.6 |
| MsG0680031473.01.T01 | AT3G17675 | 35.593 | 118 | 71 | 4 | 6 | 120 | 12 | 127 | 7.75e-18 | 75.9 |
| MsG0680031473.01.T01 | AT3G60280 | 38.614 | 101 | 61 | 1 | 20 | 120 | 19 | 118 | 1.07e-17 | 77.8 |
| MsG0680031473.01.T01 | AT1G45063 | 37.963 | 108 | 62 | 4 | 19 | 121 | 141 | 248 | 1.17e-17 | 79.7 |
| MsG0680031473.01.T01 | AT1G45063 | 31.304 | 115 | 77 | 2 | 8 | 120 | 12 | 126 | 1.82e-11 | 62.0 |
| MsG0680031473.01.T01 | AT1G22480 | 42.045 | 88 | 50 | 1 | 33 | 120 | 27 | 113 | 1.21e-17 | 76.6 |
| MsG0680031473.01.T01 | AT2G25060 | 38.462 | 143 | 73 | 7 | 6 | 141 | 19 | 153 | 4.06e-17 | 75.5 |
| MsG0680031473.01.T01 | AT5G20230 | 29.032 | 155 | 92 | 3 | 24 | 160 | 24 | 178 | 2.91e-16 | 73.6 |
| MsG0680031473.01.T01 | AT4G28365 | 32.353 | 170 | 98 | 7 | 27 | 180 | 31 | 199 | 3.95e-16 | 73.2 |
| MsG0680031473.01.T01 | AT2G44790 | 40.000 | 90 | 51 | 2 | 33 | 120 | 36 | 124 | 5.19e-16 | 72.8 |
| MsG0680031473.01.T01 | AT4G12880 | 32.479 | 117 | 74 | 4 | 7 | 120 | 11 | 125 | 8.10e-16 | 70.9 |
| MsG0680031473.01.T01 | AT5G57920 | 31.655 | 139 | 83 | 5 | 18 | 149 | 53 | 186 | 1.29e-15 | 72.0 |
| MsG0680031473.01.T01 | AT5G57920 | 30.973 | 113 | 76 | 1 | 39 | 149 | 42 | 154 | 3.05e-15 | 70.5 |
| MsG0680031473.01.T01 | AT5G14345 | 33.333 | 135 | 78 | 5 | 7 | 135 | 1 | 129 | 3.77e-15 | 69.3 |
| MsG0680031473.01.T01 | AT5G57920 | 30.973 | 113 | 76 | 1 | 39 | 149 | 41 | 153 | 3.80e-15 | 70.1 |
| MsG0680031473.01.T01 | AT2G23990 | 38.679 | 106 | 57 | 5 | 26 | 124 | 28 | 132 | 1.47e-14 | 69.3 |
| MsG0680031473.01.T01 | AT1G08500 | 29.070 | 172 | 101 | 3 | 3 | 153 | 8 | 179 | 1.87e-14 | 69.3 |
| MsG0680031473.01.T01 | AT3G53330 | 35.354 | 99 | 61 | 2 | 25 | 120 | 188 | 286 | 6.02e-14 | 68.9 |
| MsG0680031473.01.T01 | AT4G32490 | 40.206 | 97 | 54 | 3 | 27 | 120 | 33 | 128 | 6.49e-14 | 67.8 |
| MsG0680031473.01.T01 | AT4G27520 | 32.203 | 118 | 74 | 4 | 8 | 120 | 12 | 128 | 1.10e-13 | 68.6 |
| MsG0680031473.01.T01 | AT4G30590 | 36.000 | 100 | 57 | 4 | 27 | 120 | 30 | 128 | 1.34e-13 | 66.2 |
| MsG0680031473.01.T01 | AT1G17800 | 31.858 | 113 | 75 | 2 | 7 | 118 | 23 | 134 | 1.71e-13 | 64.7 |
| MsG0680031473.01.T01 | AT1G64640 | 31.496 | 127 | 81 | 4 | 19 | 141 | 27 | 151 | 1.93e-13 | 65.9 |
| MsG0680031473.01.T01 | AT4G01380 | 33.333 | 111 | 67 | 4 | 18 | 121 | 63 | 173 | 2.77e-13 | 65.9 |
| MsG0680031473.01.T01 | AT5G25090 | 31.667 | 120 | 72 | 3 | 6 | 120 | 12 | 126 | 8.62e-13 | 63.9 |
| MsG0680031473.01.T01 | AT2G02850 | 32.773 | 119 | 73 | 5 | 4 | 119 | 13 | 127 | 1.32e-12 | 62.4 |
| MsG0680031473.01.T01 | AT3G20570 | 30.400 | 125 | 79 | 3 | 8 | 124 | 13 | 137 | 1.42e-11 | 60.8 |
| MsG0680031473.01.T01 | AT1G79800 | 28.169 | 142 | 91 | 5 | 5 | 137 | 11 | 150 | 7.28e-11 | 58.9 |
Find 57 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACATTCACTTACAATATA+TGG | 0.232970 | 6:-20912477 | MsG0680031472.01.T01:intron |
| TTAATTGATTATATGAAGTT+TGG | 0.286852 | 6:+20912302 | MsG0680031473.01.T01:exon |
| TGGAAAAGATGTTGTGAAAT+TGG | 0.298026 | 6:+20912014 | MsG0680031473.01.T01:CDS |
| GACTTTGATTCATGGAGTTC+AGG | 0.309655 | 6:+20911314 | MsG0680031473.01.T01:CDS |
| CATCCTCAGACTTTGATTCA+TGG | 0.350091 | 6:+20911306 | MsG0680031473.01.T01:CDS |
| CAATACCTCCTTTGTGATCA+AGG | 0.383649 | 6:-20911243 | None:intergenic |
| CTTGATCACAAAGGAGGTAT+TGG | 0.426661 | 6:+20911244 | MsG0680031473.01.T01:CDS |
| ATCACTGTAGGGAATGGAAA+TGG | 0.451430 | 6:+20912105 | MsG0680031473.01.T01:CDS |
| CCAGTGCTTAAGGAATTTAG+TGG | 0.467341 | 6:-20911994 | None:intergenic |
| CGGGGATGATGCAGAGGAAG+AGG | 0.479314 | 6:-20912130 | MsG0680031472.01.T01:CDS |
| TACTTCACATGTGGTACCTT+AGG | 0.496106 | 6:+20912054 | MsG0680031473.01.T01:CDS |
| TCAGGTCAAACATTCAAAGT+TGG | 0.497486 | 6:+20911332 | MsG0680031473.01.T01:CDS |
| CAAAGAACAAATTGAACCAT+GGG | 0.498609 | 6:+20911185 | MsG0680031473.01.T01:exon |
| GTGATGATGACGACGACGAC+GGG | 0.505420 | 6:-20912149 | MsG0680031472.01.T01:CDS |
| ATGAAGGTAAAGATCACTGT+AGG | 0.509145 | 6:+20912093 | MsG0680031473.01.T01:CDS |
| CAGTGCTTAAGGAATTTAGT+GGG | 0.511985 | 6:-20911993 | None:intergenic |
| CATAATGTTGGTGGAAGTCA+AGG | 0.516548 | 6:+20911275 | MsG0680031473.01.T01:CDS |
| GAAGATGCATCAGAAGATGC+AGG | 0.519890 | 6:-20912210 | MsG0680031472.01.T01:CDS |
| TTCAAAGTTGGAGATCAACT+TGG | 0.523708 | 6:+20911344 | MsG0680031473.01.T01:CDS |
| CCACTAAATTCCTTAAGCAC+TGG | 0.524379 | 6:+20911994 | MsG0680031473.01.T01:CDS |
| ACCTTAGGTCATTGTGGTCA+AGG | 0.528788 | 6:+20912069 | MsG0680031473.01.T01:CDS |
| GTAAAGATCACTGTAGGGAA+TGG | 0.532845 | 6:+20912099 | MsG0680031473.01.T01:CDS |
| ACCTTGACCACAATGACCTA+AGG | 0.541983 | 6:-20912070 | None:intergenic |
| TGTTGGTGGAAGTCAAGGTT+GGG | 0.547956 | 6:+20911280 | MsG0680031473.01.T01:CDS |
| TCATTGTGGTCAAGGTATGA+AGG | 0.554331 | 6:+20912077 | MsG0680031473.01.T01:CDS |
| AATTATGTTCTTGCACCCCA+TGG | 0.558852 | 6:-20911201 | None:intergenic |
| CATGAATCAAAGTCTGAGGA+TGG | 0.566327 | 6:-20911305 | None:intergenic |
| ATACATATACATACACTAGA+GGG | 0.568007 | 6:+20912345 | MsG0680031473.01.T01:three_prime_UTR |
| CATGTGAAGTACCTTGTACC+TGG | 0.571913 | 6:-20912042 | None:intergenic |
| ATGTTGGTGGAAGTCAAGGT+TGG | 0.572733 | 6:+20911279 | MsG0680031473.01.T01:CDS |
| GCAACACAACATAATGTTGG+TGG | 0.579218 | 6:+20911266 | MsG0680031473.01.T01:CDS |
| CATGTTTGCTGAATATGAAG+AGG | 0.592143 | 6:-20912395 | MsG0680031472.01.T01:intron |
| AAGATGCATCAGAAGATGCA+GGG | 0.597177 | 6:-20912209 | MsG0680031472.01.T01:CDS |
| ACAAAGAACAAATTGAACCA+TGG | 0.598455 | 6:+20911184 | MsG0680031473.01.T01:exon |
| CAAAACAATCTCTAAAAGAG+TGG | 0.607452 | 6:+20911131 | MsG0680031473.01.T01:five_prime_UTR |
| TTGGCAACACAACATAATGT+TGG | 0.615720 | 6:+20911263 | MsG0680031473.01.T01:CDS |
| GGTGATGATGACGACGACGA+CGG | 0.616145 | 6:-20912150 | MsG0680031472.01.T01:CDS |
| TGAAGGTAAAGATCACTGTA+GGG | 0.620720 | 6:+20912094 | MsG0680031473.01.T01:CDS |
| AATAGAAGCAAAGTATTGTG+AGG | 0.621549 | 6:-20912235 | MsG0680031472.01.T01:CDS |
| AATTGGACAAGCCAGGTACA+AGG | 0.622027 | 6:+20912031 | MsG0680031473.01.T01:CDS |
| ACTAGAGGGGTACAGTGGCA+TGG | 0.623232 | 6:+20912359 | MsG0680031473.01.T01:three_prime_UTR |
| ACTCCATGAATCAAAGTCTG+AGG | 0.627608 | 6:-20911309 | None:intergenic |
| CGACGACGGGGATGATGCAG+AGG | 0.629341 | 6:-20912136 | MsG0680031472.01.T01:CDS |
| TGTGGTACCTTAGGTCATTG+TGG | 0.638088 | 6:+20912063 | MsG0680031473.01.T01:CDS |
| TATACATATACATACACTAG+AGG | 0.644164 | 6:+20912344 | MsG0680031473.01.T01:three_prime_UTR |
| GTTGTGAAATTGGACAAGCC+AGG | 0.645162 | 6:+20912024 | MsG0680031473.01.T01:CDS |
| AGCTACCTTGATCACAAAGG+AGG | 0.662032 | 6:+20911238 | MsG0680031473.01.T01:CDS |
| AGTGCTTAAGGAATTTAGTG+GGG | 0.662048 | 6:-20911992 | None:intergenic |
| GTTAGCTACCTTGATCACAA+AGG | 0.669540 | 6:+20911235 | MsG0680031473.01.T01:CDS |
| AGATGCATCAGAAGATGCAG+GGG | 0.674445 | 6:-20912208 | MsG0680031472.01.T01:CDS |
| GGTACAAGGTACTTCACATG+TGG | 0.691210 | 6:+20912045 | MsG0680031473.01.T01:CDS |
| CATACACTAGAGGGGTACAG+TGG | 0.691833 | 6:+20912354 | MsG0680031473.01.T01:three_prime_UTR |
| TACATATACATACACTAGAG+GGG | 0.692551 | 6:+20912346 | MsG0680031473.01.T01:three_prime_UTR |
| TGATGATGACGACGACGACG+GGG | 0.741875 | 6:-20912148 | MsG0680031472.01.T01:CDS |
| AAAGAACAAATTGAACCATG+GGG | 0.757129 | 6:+20911186 | MsG0680031473.01.T01:exon |
| GTACTCATCTATGCACAGTG+TGG | 0.766087 | 6:+20911924 | MsG0680031473.01.T01:CDS |
| GATGACGATGAAGATGACGA+TGG | 0.781610 | 6:-20912171 | MsG0680031472.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCAAAATTTATAACCT+TGG | + | Chr6:20911804-20911823 | MsG0680031473.01.T01:intron | 15.0% |
| !! | TTAATCATCACTTTAAATTA+AGG | + | Chr6:20911465-20911484 | MsG0680031473.01.T01:intron | 15.0% |
| !! | TTAATTGATTATATGAAGTT+TGG | + | Chr6:20912302-20912321 | MsG0680031473.01.T01:exon | 15.0% |
| !!! | TCACTTTAATTATTTTTACA+TGG | + | Chr6:20911398-20911417 | MsG0680031473.01.T01:intron | 15.0% |
| !! | GTTTCTTATCAAAAATTTGT+TGG | + | Chr6:20911370-20911389 | MsG0680031473.01.T01:intron | 20.0% |
| !!! | TTTTTTCTACTTTCTACTAT+AGG | - | Chr6:20911434-20911453 | None:intergenic | 20.0% |
| ! | AACTCAATTGTAGCAATTTA+TGG | + | Chr6:20911619-20911638 | MsG0680031473.01.T01:intron | 25.0% |
| ! | ACACATTCACTTACAATATA+TGG | - | Chr6:20912480-20912499 | MsG0680031472.01.T01:intron | 25.0% |
| ! | ATACATATACATACACTAGA+GGG | + | Chr6:20912345-20912364 | MsG0680031473.01.T01:three_prime_UTR | 25.0% |
| ! | TAAACAATGGTATTGTAGAT+GGG | + | Chr6:20911566-20911585 | MsG0680031473.01.T01:intron | 25.0% |
| ! | TATACATATACATACACTAG+AGG | + | Chr6:20912344-20912363 | MsG0680031473.01.T01:three_prime_UTR | 25.0% |
| ! | TTAAACAATGGTATTGTAGA+TGG | + | Chr6:20911565-20911584 | MsG0680031473.01.T01:intron | 25.0% |
| !! | CCATTGTTTAAAACTTTGAT+TGG | - | Chr6:20911556-20911575 | None:intergenic | 25.0% |
| !! | TATTTTCATAATCACATCCA+AGG | - | Chr6:20911824-20911843 | None:intergenic | 25.0% |
| !! | TTGTTTAAAACTTTGATTGG+TGG | - | Chr6:20911553-20911572 | None:intergenic | 25.0% |
| !!! | CAGTTTTAGTAATATTGTGA+TGG | + | Chr6:20911497-20911516 | MsG0680031473.01.T01:intron | 25.0% |
| !!! | CCAATCAAAGTTTTAAACAA+TGG | + | Chr6:20911553-20911572 | MsG0680031473.01.T01:intron | 25.0% |
| !!! | GCTAATATCACATTTTTTGT+AGG | - | Chr6:20911971-20911990 | None:intergenic | 25.0% |
| AAAGAACAAATTGAACCATG+GGG | + | Chr6:20911186-20911205 | MsG0680031473.01.T01:exon | 30.0% | |
| ACAAAGAACAAATTGAACCA+TGG | + | Chr6:20911184-20911203 | MsG0680031473.01.T01:exon | 30.0% | |
| CAAAGAACAAATTGAACCAT+GGG | + | Chr6:20911185-20911204 | MsG0680031473.01.T01:exon | 30.0% | |
| TACATATACATACACTAGAG+GGG | + | Chr6:20912346-20912365 | MsG0680031473.01.T01:three_prime_UTR | 30.0% | |
| TGGAAAAGATGTTGTGAAAT+TGG | + | Chr6:20912014-20912033 | MsG0680031473.01.T01:CDS | 30.0% | |
| TTATGGTTGAATAACTCAAC+TGG | + | Chr6:20911636-20911655 | MsG0680031473.01.T01:intron | 30.0% | |
| ! | CAAAACAATCTCTAAAAGAG+TGG | + | Chr6:20911131-20911150 | MsG0680031473.01.T01:five_prime_UTR | 30.0% |
| ! | GTGCAAGAACATAATTTTCT+TGG | + | Chr6:20911208-20911227 | MsG0680031473.01.T01:CDS | 30.0% |
| !! | AATAGAAGCAAAGTATTGTG+AGG | - | Chr6:20912238-20912257 | MsG0680031472.01.T01:CDS | 30.0% |
| !!! | ATGATACTGCCACAATTTTT+TGG | - | Chr6:20911748-20911767 | None:intergenic | 30.0% |
| AGTGCTTAAGGAATTTAGTG+GGG | - | Chr6:20911995-20912014 | None:intergenic | 35.0% | |
| ATGAAGGTAAAGATCACTGT+AGG | + | Chr6:20912093-20912112 | MsG0680031473.01.T01:CDS | 35.0% | |
| CAGTGCTTAAGGAATTTAGT+GGG | - | Chr6:20911996-20912015 | None:intergenic | 35.0% | |
| TACGTCGATCCAAAAAATTG+TGG | + | Chr6:20911736-20911755 | MsG0680031473.01.T01:intron | 35.0% | |
| TCAGGTCAAACATTCAAAGT+TGG | + | Chr6:20911332-20911351 | MsG0680031473.01.T01:CDS | 35.0% | |
| TGAAGGTAAAGATCACTGTA+GGG | + | Chr6:20912094-20912113 | MsG0680031473.01.T01:CDS | 35.0% | |
| TTCAAAGTTGGAGATCAACT+TGG | + | Chr6:20911344-20911363 | MsG0680031473.01.T01:CDS | 35.0% | |
| TTGGCAACACAACATAATGT+TGG | + | Chr6:20911263-20911282 | MsG0680031473.01.T01:CDS | 35.0% | |
| ! | AACATCTTTTCCAGTGCTTA+AGG | - | Chr6:20912007-20912026 | None:intergenic | 35.0% |
| ! | CATGTTTGCTGAATATGAAG+AGG | - | Chr6:20912398-20912417 | MsG0680031472.01.T01:intron | 35.0% |
| !! | AACTCAACTGGTTTGAACTT+AGG | + | Chr6:20911648-20911667 | MsG0680031473.01.T01:intron | 35.0% |
| !! | ACTCAACTGGTTTGAACTTA+GGG | + | Chr6:20911649-20911668 | MsG0680031473.01.T01:intron | 35.0% |
| AAGATGCATCAGAAGATGCA+GGG | - | Chr6:20912212-20912231 | MsG0680031472.01.T01:CDS | 40.0% | |
| AATTATGTTCTTGCACCCCA+TGG | - | Chr6:20911204-20911223 | None:intergenic | 40.0% | |
| ACTCCATGAATCAAAGTCTG+AGG | - | Chr6:20911312-20911331 | None:intergenic | 40.0% | |
| ATCACTGTAGGGAATGGAAA+TGG | + | Chr6:20912105-20912124 | MsG0680031473.01.T01:CDS | 40.0% | |
| CAATACCTCCTTTGTGATCA+AGG | - | Chr6:20911246-20911265 | None:intergenic | 40.0% | |
| CATCCTCAGACTTTGATTCA+TGG | + | Chr6:20911306-20911325 | MsG0680031473.01.T01:CDS | 40.0% | |
| CATGAATCAAAGTCTGAGGA+TGG | - | Chr6:20911308-20911327 | None:intergenic | 40.0% | |
| CCACTAAATTCCTTAAGCAC+TGG | + | Chr6:20911994-20912013 | MsG0680031473.01.T01:CDS | 40.0% | |
| CCAGTGCTTAAGGAATTTAG+TGG | - | Chr6:20911997-20912016 | None:intergenic | 40.0% | |
| GACTTTGATTCATGGAGTTC+AGG | + | Chr6:20911314-20911333 | MsG0680031473.01.T01:CDS | 40.0% | |
| GCAACACAACATAATGTTGG+TGG | + | Chr6:20911266-20911285 | MsG0680031473.01.T01:CDS | 40.0% | |
| GTAAAGATCACTGTAGGGAA+TGG | + | Chr6:20912099-20912118 | MsG0680031473.01.T01:CDS | 40.0% | |
| GTTAGCTACCTTGATCACAA+AGG | + | Chr6:20911235-20911254 | MsG0680031473.01.T01:CDS | 40.0% | |
| TACTTCACATGTGGTACCTT+AGG | + | Chr6:20912054-20912073 | MsG0680031473.01.T01:CDS | 40.0% | |
| TCATTGTGGTCAAGGTATGA+AGG | + | Chr6:20912077-20912096 | MsG0680031473.01.T01:CDS | 40.0% | |
| ! | CATAATGTTGGTGGAAGTCA+AGG | + | Chr6:20911275-20911294 | MsG0680031473.01.T01:CDS | 40.0% |
| ! | CTTGATCACAAAGGAGGTAT+TGG | + | Chr6:20911244-20911263 | MsG0680031473.01.T01:CDS | 40.0% |
| AATTGGACAAGCCAGGTACA+AGG | + | Chr6:20912031-20912050 | MsG0680031473.01.T01:CDS | 45.0% | |
| ACCTTAGGTCATTGTGGTCA+AGG | + | Chr6:20912069-20912088 | MsG0680031473.01.T01:CDS | 45.0% | |
| ACCTTGACCACAATGACCTA+AGG | - | Chr6:20912073-20912092 | None:intergenic | 45.0% | |
| AGATGCATCAGAAGATGCAG+GGG | - | Chr6:20912211-20912230 | MsG0680031472.01.T01:CDS | 45.0% | |
| AGCTACCTTGATCACAAAGG+AGG | + | Chr6:20911238-20911257 | MsG0680031473.01.T01:CDS | 45.0% | |
| GAAGATGCATCAGAAGATGC+AGG | - | Chr6:20912213-20912232 | MsG0680031472.01.T01:CDS | 45.0% | |
| GATGACGATGAAGATGACGA+TGG | - | Chr6:20912174-20912193 | MsG0680031472.01.T01:CDS | 45.0% | |
| GTACTCATCTATGCACAGTG+TGG | + | Chr6:20911924-20911943 | MsG0680031473.01.T01:CDS | 45.0% | |
| GTTGTGAAATTGGACAAGCC+AGG | + | Chr6:20912024-20912043 | MsG0680031473.01.T01:CDS | 45.0% | |
| TGTGGTACCTTAGGTCATTG+TGG | + | Chr6:20912063-20912082 | MsG0680031473.01.T01:CDS | 45.0% | |
| ! | ATGTTGGTGGAAGTCAAGGT+TGG | + | Chr6:20911279-20911298 | MsG0680031473.01.T01:CDS | 45.0% |
| ! | CATGTGAAGTACCTTGTACC+TGG | - | Chr6:20912045-20912064 | None:intergenic | 45.0% |
| ! | GGTACAAGGTACTTCACATG+TGG | + | Chr6:20912045-20912064 | MsG0680031473.01.T01:CDS | 45.0% |
| ! | TGTTGGTGGAAGTCAAGGTT+GGG | + | Chr6:20911280-20911299 | MsG0680031473.01.T01:CDS | 45.0% |
| ! | CATACACTAGAGGGGTACAG+TGG | + | Chr6:20912354-20912373 | MsG0680031473.01.T01:three_prime_UTR | 50.0% |
| ! | ACTAGAGGGGTACAGTGGCA+TGG | + | Chr6:20912359-20912378 | MsG0680031473.01.T01:three_prime_UTR | 55.0% |
| ! | GGTGATGATGACGACGACGA+CGG | - | Chr6:20912153-20912172 | MsG0680031472.01.T01:CDS | 55.0% |
| ! | GTGATGATGACGACGACGAC+GGG | - | Chr6:20912152-20912171 | MsG0680031472.01.T01:CDS | 55.0% |
| ! | TGATGATGACGACGACGACG+GGG | - | Chr6:20912151-20912170 | MsG0680031472.01.T01:CDS | 55.0% |
| CGGGGATGATGCAGAGGAAG+AGG | - | Chr6:20912133-20912152 | MsG0680031472.01.T01:CDS | 60.0% | |
| CGACGACGGGGATGATGCAG+AGG | - | Chr6:20912139-20912158 | MsG0680031472.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 20911129 | 20912531 | 20911129 | ID=MsG0680031473.01;Name=MsG0680031473.01 |
| Chr6 | mRNA | 20911129 | 20912531 | 20911129 | ID=MsG0680031473.01.T01;Parent=MsG0680031473.01;Name=MsG0680031473.01.T01;_AED=0.48;_eAED=0.48;_QI=74|1|1|1|0|0|2|226|183 |
| Chr6 | exon | 20911129 | 20911365 | 20911129 | ID=MsG0680031473.01.T01:exon:13199;Parent=MsG0680031473.01.T01 |
| Chr6 | exon | 20911917 | 20912531 | 20911917 | ID=MsG0680031473.01.T01:exon:13200;Parent=MsG0680031473.01.T01 |
| Chr6 | five_prime_UTR | 20911129 | 20911202 | 20911129 | ID=MsG0680031473.01.T01:five_prime_utr;Parent=MsG0680031473.01.T01 |
| Chr6 | CDS | 20911203 | 20911365 | 20911203 | ID=MsG0680031473.01.T01:cds;Parent=MsG0680031473.01.T01 |
| Chr6 | CDS | 20911917 | 20912305 | 20911917 | ID=MsG0680031473.01.T01:cds;Parent=MsG0680031473.01.T01 |
| Chr6 | three_prime_UTR | 20912306 | 20912531 | 20912306 | ID=MsG0680031473.01.T01:three_prime_utr;Parent=MsG0680031473.01.T01 |
| Gene Sequence |
| Protein sequence |
