AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035247.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035247.01.T01 MTR_3g099570 78.261 138 26 2 1 136 1 136 2.01e-69 209
MsG0680035247.01.T01 MTR_3g099580 80.992 121 23 0 1 121 1 121 1.12e-67 204
MsG0680035247.01.T01 MTR_3g099680 78.049 123 25 2 1 121 1 123 7.42e-61 188
MsG0680035247.01.T01 MTR_3g099980 78.689 122 24 2 1 120 1 122 2.44e-60 187
MsG0680035247.01.T01 MTR_3g099540 66.412 131 43 1 1 130 1 131 1.76e-53 166
MsG0680035247.01.T01 MTR_8g007020 49.306 144 59 4 4 134 2 144 1.03e-35 123
MsG0680035247.01.T01 MTR_8g007035 50.820 122 57 2 5 123 2 123 1.08e-35 124
MsG0680035247.01.T01 MTR_1g090190 38.462 130 78 2 23 152 20 147 3.41e-27 101
MsG0680035247.01.T01 MTR_5g006040 42.308 130 71 4 9 134 8 137 4.66e-27 101
MsG0680035247.01.T01 MTR_4g114870 43.796 137 70 4 5 134 6 142 5.96e-26 102
MsG0680035247.01.T01 MTR_4g114870 49.000 100 46 2 26 120 191 290 1.85e-24 98.2
MsG0680035247.01.T01 MTR_2g088990 35.811 148 92 3 1 146 1 147 5.30e-24 94.4
MsG0680035247.01.T01 MTR_6g080660 44.554 101 56 0 26 126 28 128 1.35e-23 92.8
MsG0680035247.01.T01 MTR_0334s0010 40.496 121 67 2 7 122 7 127 8.31e-23 90.5
MsG0680035247.01.T01 MTR_1g104800 41.748 103 57 3 21 120 20 122 5.19e-22 88.2
MsG0680035247.01.T01 MTR_1g112700 31.250 160 96 4 10 161 11 164 6.46e-22 87.8
MsG0680035247.01.T01 MTR_6g023760 42.000 100 57 1 22 120 22 121 2.00e-20 84.0
MsG0680035247.01.T01 MTR_6g083240 35.570 149 87 5 5 146 6 152 3.38e-20 83.2
MsG0680035247.01.T01 MTR_1g105130 37.600 125 69 4 1 120 1 121 8.56e-20 81.6
MsG0680035247.01.T01 MTR_1g105120 37.600 125 69 4 1 120 1 121 8.56e-20 81.6
MsG0680035247.01.T01 MTR_2g043730 43.137 102 54 3 21 121 27 125 9.75e-20 80.5
MsG0680035247.01.T01 MTR_7g086140 39.167 120 68 3 9 123 6 125 1.43e-19 81.3
MsG0680035247.01.T01 MTR_7g086190 39.167 120 68 3 9 123 6 125 1.43e-19 81.3
MsG0680035247.01.T01 MTR_4g067200 36.508 126 73 3 6 124 8 133 5.55e-19 79.7
MsG0680035247.01.T01 MTR_4g066110 36.508 126 73 3 6 124 8 133 5.55e-19 79.7
MsG0680035247.01.T01 MTR_8g099220 37.288 118 70 2 7 120 10 127 1.24e-18 77.8
MsG0680035247.01.T01 MTR_7g086160 36.975 119 73 2 7 123 8 126 1.86e-18 78.2
MsG0680035247.01.T01 MTR_7g086230 38.525 122 74 1 1 121 1 122 1.92e-18 78.6
MsG0680035247.01.T01 MTR_3g089035 45.361 97 49 3 23 118 30 123 2.04e-18 77.0
MsG0680035247.01.T01 MTR_7g086100 36.364 121 76 1 1 120 1 121 2.13e-17 77.0
MsG0680035247.01.T01 MTR_7g086280 37.705 122 75 1 1 121 1 122 2.30e-17 75.9
MsG0680035247.01.T01 MTR_7g086090 36.885 122 76 1 1 121 1 122 2.54e-17 77.0
MsG0680035247.01.T01 MTR_7g086220 37.615 109 64 2 6 110 2 110 3.38e-17 75.1
MsG0680035247.01.T01 MTR_4g007250 40.196 102 59 2 20 120 23 123 2.49e-16 72.8
MsG0680035247.01.T01 MTR_2g025580 30.894 123 83 1 1 121 2 124 1.04e-15 71.2
MsG0680035247.01.T01 MTR_8g089110 35.833 120 73 3 1 119 3 119 1.32e-15 69.7
MsG0680035247.01.T01 MTR_1g090420 30.233 129 84 3 23 145 25 153 3.31e-15 70.9
MsG0680035247.01.T01 MTR_2g043790 42.308 104 54 4 21 123 2 100 5.42e-15 67.4
MsG0680035247.01.T01 MTR_4g081100 36.290 124 72 3 4 120 8 131 6.27e-15 70.1
MsG0680035247.01.T01 MTR_2g083250 39.623 106 57 2 28 126 28 133 7.24e-15 70.9
MsG0680035247.01.T01 MTR_4g130800 33.071 127 78 4 1 120 3 129 3.49e-14 68.9
MsG0680035247.01.T01 MTR_8g088830 38.776 98 55 4 23 118 25 119 1.47e-13 64.3
MsG0680035247.01.T01 MTR_2g101300 34.307 137 78 4 15 140 11 146 2.58e-13 65.5
MsG0680035247.01.T01 MTR_8g463180 33.083 133 82 4 11 139 8 137 3.50e-13 66.6
MsG0680035247.01.T01 MTR_6g013420 34.848 132 77 6 7 134 20 146 1.08e-12 63.5
MsG0680035247.01.T01 MTR_4g130800 38.272 81 49 1 41 120 48 128 1.42e-12 64.3
MsG0680035247.01.T01 MTR_6g013200 33.051 118 74 4 7 120 15 131 1.78e-12 62.8
MsG0680035247.01.T01 MTR_8g086360 33.577 137 80 4 28 153 26 162 2.84e-12 62.4
MsG0680035247.01.T01 MTR_4g111640 31.148 122 72 4 10 120 11 131 3.12e-12 62.4
MsG0680035247.01.T01 MTR_6g022170 34.951 103 61 3 23 120 31 132 3.62e-12 61.6
MsG0680035247.01.T01 MTR_4g114830 37.963 108 58 5 17 115 18 125 8.18e-12 60.8
MsG0680035247.01.T01 MTR_2g090580 37.500 96 57 2 28 120 29 124 9.70e-12 61.2
MsG0680035247.01.T01 MTR_2g090575 37.500 96 57 2 28 120 27 122 1.28e-11 62.4
MsG0680035247.01.T01 MTR_1g014120 33.962 106 65 2 29 129 29 134 2.05e-11 60.1
MsG0680035247.01.T01 MTR_4g077787 30.159 126 80 3 2 120 7 131 3.18e-11 59.7
MsG0680035247.01.T01 MTR_4g130780 27.941 136 91 4 1 129 3 138 5.09e-11 58.9
MsG0680035247.01.T01 MTR_4g112310 33.333 81 53 1 40 120 2 81 6.28e-11 57.8
MsG0680035247.01.T01 MTR_8g094990 34.821 112 69 2 27 134 26 137 8.12e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035247.01.T01 AT1G72230 48.611 144 66 2 3 146 1 136 3.85e-39 132
MsG0680035247.01.T01 AT1G22480 57.292 96 41 0 31 126 24 119 1.57e-32 115
MsG0680035247.01.T01 AT2G32300 38.415 164 93 3 1 163 1 157 1.26e-28 107
MsG0680035247.01.T01 AT2G32300 41.481 135 78 1 1 134 31 165 2.28e-28 107
MsG0680035247.01.T01 AT3G60270 47.059 102 54 0 22 123 22 123 3.37e-27 101
MsG0680035247.01.T01 AT5G07475 40.336 119 71 0 23 141 27 145 2.49e-26 99.8
MsG0680035247.01.T01 AT2G31050 40.741 135 72 4 3 133 10 140 3.39e-24 94.4
MsG0680035247.01.T01 AT2G44790 51.613 93 43 1 34 124 36 128 1.81e-23 92.4
MsG0680035247.01.T01 AT2G26720 44.231 104 56 2 23 124 26 129 1.89e-23 92.4
MsG0680035247.01.T01 AT3G27200 37.190 121 75 1 1 120 1 121 4.15e-23 90.9
MsG0680035247.01.T01 AT5G26330 36.970 165 93 5 23 176 21 185 2.14e-22 89.4
MsG0680035247.01.T01 AT3G60280 43.137 102 58 0 22 123 20 121 5.65e-21 86.3
MsG0680035247.01.T01 AT3G17675 35.833 120 75 2 3 120 8 127 2.81e-20 82.0
MsG0680035247.01.T01 AT5G20230 37.398 123 63 3 26 134 25 147 2.26e-15 70.9
MsG0680035247.01.T01 AT5G15350 37.288 118 71 3 6 121 10 126 3.68e-15 70.1
MsG0680035247.01.T01 AT4G12880 35.484 124 75 4 1 121 5 126 7.75e-15 68.2
MsG0680035247.01.T01 AT4G27520 32.812 128 78 3 1 120 1 128 4.50e-14 69.3
MsG0680035247.01.T01 AT1G64640 35.537 121 73 3 23 139 30 149 9.08e-14 66.6
MsG0680035247.01.T01 AT2G25060 39.167 120 66 5 27 139 32 151 1.39e-13 65.9
MsG0680035247.01.T01 AT5G53870 31.126 151 87 4 2 140 3 148 1.68e-13 67.8
MsG0680035247.01.T01 AT5G53870 31.126 151 87 4 2 140 3 148 2.28e-13 67.4
MsG0680035247.01.T01 AT4G31840 34.014 147 83 7 4 139 3 146 2.52e-13 65.1
MsG0680035247.01.T01 AT3G18590 34.711 121 65 3 13 120 7 126 3.60e-13 65.1
MsG0680035247.01.T01 AT1G45063 36.522 115 63 5 25 133 148 258 1.03e-12 65.1
MsG0680035247.01.T01 AT1G45063 35.294 119 67 5 25 137 148 262 1.23e-12 65.5
MsG0680035247.01.T01 AT4G28365 36.458 96 58 2 28 120 31 126 1.25e-12 63.5
MsG0680035247.01.T01 AT3G53330 30.159 126 84 3 22 143 184 309 4.56e-12 63.5
MsG0680035247.01.T01 AT2G02850 38.144 97 56 3 23 118 33 126 7.19e-12 60.1
MsG0680035247.01.T01 AT2G27035 28.125 128 88 4 6 130 8 134 1.24e-11 60.5
MsG0680035247.01.T01 AT5G14345 30.469 128 80 3 12 131 2 128 1.29e-11 60.1
MsG0680035247.01.T01 AT4G30590 27.869 122 83 2 7 123 10 131 1.53e-11 60.5
MsG0680035247.01.T01 AT3G20570 33.613 119 72 3 8 122 15 130 2.08e-11 60.5
MsG0680035247.01.T01 AT4G01380 27.642 123 79 4 31 145 77 197 7.00e-11 59.3

Find 38 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATGACAGTGTTCAATTA+TGG 0.235040 6:-101032259 MsG0680035247.01.T01:intron
ACAGTGTTCAATTATGGAAT+TGG 0.298388 6:-101032253 MsG0680035247.01.T01:intron
TACTTCATATGCGCTGTTCC+TGG 0.318286 6:-101032115 MsG0680035247.01.T01:CDS
GCTCCTTCTGCAACACCATC+AGG 0.341751 6:-101032034 MsG0680035247.01.T01:CDS
AATGACAAAACCTTTGTAGT+TGG 0.413140 6:-101032430 MsG0680035247.01.T01:CDS
CAGTGTTCAATTATGGAATT+GGG 0.428478 6:-101032252 MsG0680035247.01.T01:CDS
GTGGAATGTGTGGTAGTTGT+TGG 0.433479 6:+101031990 None:intergenic
CACACCGTTGGAGATACTTC+AGG 0.449866 6:-101032487 MsG0680035247.01.T01:CDS
TGGCATGAAACTCTCTGTTA+AGG 0.450378 6:-101032080 MsG0680035247.01.T01:CDS
GATACTTCAGGTTGGACAAT+CGG 0.457701 6:-101032475 MsG0680035247.01.T01:CDS
TGTGTGGTAGTTGTTGGGCA+AGG 0.459616 6:+101031996 None:intergenic
GGTAGGAGTGGTGGTGGTCG+TGG 0.462242 6:+101031971 None:intergenic
TTTGCAACTGTCCACACCGT+TGG 0.509170 6:-101032499 MsG0680035247.01.T01:CDS
TTTGTAGTTGGAGACAGTCT+TGG 0.511925 6:-101032418 MsG0680035247.01.T01:intron
ACACCATCAGGGAAAGTTGA+TGG 0.512811 6:-101032022 MsG0680035247.01.T01:CDS
TTCCCTGATGGTGTTGCAGA+AGG 0.516823 6:+101032031 None:intergenic
TGGAATGTGTGGTAGTTGTT+GGG 0.519162 6:+101031991 None:intergenic
GAAGTTGCACTTTGCGTGGT+AGG 0.529660 6:+101031954 None:intergenic
GTTCCTGGACATTGCGTATG+TGG 0.535150 6:-101032100 MsG0680035247.01.T01:CDS
ATGCCACATACGCAATGTCC+AGG 0.545019 6:+101032097 None:intergenic
GTGTGGACAGTTGCAAAAGT+TGG 0.551081 6:+101032505 None:intergenic
AGCTGAAGTTGCACTTTGCG+TGG 0.552340 6:+101031950 None:intergenic
GTGTGGTAGTTGTTGGGCAA+GGG 0.559907 6:+101031997 None:intergenic
CTCCTTCTGCAACACCATCA+GGG 0.584013 6:-101032033 MsG0680035247.01.T01:CDS
GTGCCATCAACTTTCCCTGA+TGG 0.588940 6:+101032019 None:intergenic
TCGGTTCTGATTATAGCACA+TGG 0.592715 6:-101032456 MsG0680035247.01.T01:CDS
CAATTATGGAATTGGGCACA+CGG 0.595099 6:-101032245 MsG0680035247.01.T01:CDS
TGCACTTTGCGTGGTAGGAG+TGG 0.601900 6:+101031959 None:intergenic
GGTGGTGGTCGTGGAATGTG+TGG 0.616937 6:+101031980 None:intergenic
TTGCGTGGTAGGAGTGGTGG+TGG 0.624187 6:+101031965 None:intergenic
AAGACTGTCTCCAACTACAA+AGG 0.647672 6:+101032420 None:intergenic
CCAACCTGAAGTATCTCCAA+CGG 0.658901 6:+101032483 None:intergenic
CCGTTGGAGATACTTCAGGT+TGG 0.662766 6:-101032483 MsG0680035247.01.T01:CDS
CGGTTCTGATTATAGCACAT+GGG 0.665310 6:-101032455 MsG0680035247.01.T01:CDS
ACTTTGCGTGGTAGGAGTGG+TGG 0.668649 6:+101031962 None:intergenic
CTGAAGTATCTCCAACGGTG+TGG 0.674320 6:+101032488 None:intergenic
TCAATTAGTACAGACAGTAG+CGG 0.713968 6:-101032175 MsG0680035247.01.T01:CDS
TTATGGAATTGGGCACACGG+TGG 0.751270 6:-101032242 MsG0680035247.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACATTAGTTTTTTAAATTA+TGG + Chr6:101032168-101032187 None:intergenic 10.0%
! ATTATGACAGTGTTCAATTA+TGG - Chr6:101032201-101032220 MsG0680035247.01.T01:CDS 25.0%
! GTGAAAAAGAAAGCAAAAAT+TGG + Chr6:101032548-101032567 None:intergenic 25.0%
AATGACAAAACCTTTGTAGT+TGG - Chr6:101032030-101032049 MsG0680035247.01.T01:CDS 30.0%
ACAGTGTTCAATTATGGAAT+TGG - Chr6:101032207-101032226 MsG0680035247.01.T01:CDS 30.0%
CAGTGTTCAATTATGGAATT+GGG - Chr6:101032208-101032227 MsG0680035247.01.T01:CDS 30.0%
!! CTTTCTTTTTCACAGTTTCA+TGG - Chr6:101032555-101032574 MsG0680035247.01.T01:CDS 30.0%
CATAGTGATCGCTAAAAAGA+AGG + Chr6:101031929-101031948 None:intergenic 35.0%
TCAATTAGTACAGACAGTAG+CGG - Chr6:101032285-101032304 MsG0680035247.01.T01:intron 35.0%
!! CTTCTTTTTAGCGATCACTA+TGG - Chr6:101031927-101031946 MsG0680035247.01.T01:CDS 35.0%
!!! TTTTTTGAAGGGAATGGTTG+TGG + Chr6:101032313-101032332 None:intergenic 35.0%
AAGACTGTCTCCAACTACAA+AGG + Chr6:101032043-101032062 None:intergenic 40.0%
ACCATTCCCTTCAAAAAAGC+AGG - Chr6:101032315-101032334 MsG0680035247.01.T01:intron 40.0%
CAATTATGGAATTGGGCACA+CGG - Chr6:101032215-101032234 MsG0680035247.01.T01:CDS 40.0%
TGGAATGTGTGGTAGTTGTT+GGG + Chr6:101032472-101032491 None:intergenic 40.0%
TGGCATGAAACTCTCTGTTA+AGG - Chr6:101032380-101032399 MsG0680035247.01.T01:intron 40.0%
TTTGTAGTTGGAGACAGTCT+TGG - Chr6:101032042-101032061 MsG0680035247.01.T01:CDS 40.0%
! CGGTTCTGATTATAGCACAT+GGG - Chr6:101032005-101032024 MsG0680035247.01.T01:CDS 40.0%
! TCGGTTCTGATTATAGCACA+TGG - Chr6:101032004-101032023 MsG0680035247.01.T01:CDS 40.0%
!! GATACTTCAGGTTGGACAAT+CGG - Chr6:101031985-101032004 MsG0680035247.01.T01:CDS 40.0%
!!! ATGCGTTCCTGCTTTTTTGA+AGG + Chr6:101032325-101032344 None:intergenic 40.0%
!!! TCCTGCTTTTTTGAAGGGAA+TGG + Chr6:101032319-101032338 None:intergenic 40.0%
!!! TGCGTTCCTGCTTTTTTGAA+GGG + Chr6:101032324-101032343 None:intergenic 40.0%
ACACCATCAGGGAAAGTTGA+TGG - Chr6:101032438-101032457 MsG0680035247.01.T01:CDS 45.0%
GTGGAATGTGTGGTAGTTGT+TGG + Chr6:101032473-101032492 None:intergenic 45.0%
GTGTGGACAGTTGCAAAAGT+TGG + Chr6:101031958-101031977 None:intergenic 45.0%
TACTTCATATGCGCTGTTCC+TGG - Chr6:101032345-101032364 MsG0680035247.01.T01:intron 45.0%
! CCAACCTGAAGTATCTCCAA+CGG + Chr6:101031980-101031999 None:intergenic 45.0%
AGCTGAAGTTGCACTTTGCG+TGG + Chr6:101032513-101032532 None:intergenic 50.0%
ATGCCACATACGCAATGTCC+AGG + Chr6:101032366-101032385 None:intergenic 50.0%
CACACCGTTGGAGATACTTC+AGG - Chr6:101031973-101031992 MsG0680035247.01.T01:CDS 50.0%
CCGTTGGAGATACTTCAGGT+TGG - Chr6:101031977-101031996 MsG0680035247.01.T01:CDS 50.0%
CTCCTTCTGCAACACCATCA+GGG - Chr6:101032427-101032446 MsG0680035247.01.T01:CDS 50.0%
GAAGTTGCACTTTGCGTGGT+AGG + Chr6:101032509-101032528 None:intergenic 50.0%
GTGCCATCAACTTTCCCTGA+TGG + Chr6:101032444-101032463 None:intergenic 50.0%
GTGTGGTAGTTGTTGGGCAA+GGG + Chr6:101032466-101032485 None:intergenic 50.0%
GTTCCTGGACATTGCGTATG+TGG - Chr6:101032360-101032379 MsG0680035247.01.T01:intron 50.0%
TGTGTGGTAGTTGTTGGGCA+AGG + Chr6:101032467-101032486 None:intergenic 50.0%
TTATGGAATTGGGCACACGG+TGG - Chr6:101032218-101032237 MsG0680035247.01.T01:CDS 50.0%
TTTGCAACTGTCCACACCGT+TGG - Chr6:101031961-101031980 MsG0680035247.01.T01:CDS 50.0%
! CTGAAGTATCTCCAACGGTG+TGG + Chr6:101031975-101031994 None:intergenic 50.0%
!! TTCCCTGATGGTGTTGCAGA+AGG + Chr6:101032432-101032451 None:intergenic 50.0%
ACTTTGCGTGGTAGGAGTGG+TGG + Chr6:101032501-101032520 None:intergenic 55.0%
GCTCCTTCTGCAACACCATC+AGG - Chr6:101032426-101032445 MsG0680035247.01.T01:CDS 55.0%
TGCACTTTGCGTGGTAGGAG+TGG + Chr6:101032504-101032523 None:intergenic 55.0%
TTGCGTGGTAGGAGTGGTGG+TGG + Chr6:101032498-101032517 None:intergenic 60.0%
! GGTGGTGGTCGTGGAATGTG+TGG + Chr6:101032483-101032502 None:intergenic 60.0%
!! GGTAGGAGTGGTGGTGGTCG+TGG + Chr6:101032492-101032511 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 101031898 101032584 101031898 ID=MsG0680035247.01;Name=MsG0680035247.01
Chr6 mRNA 101031898 101032584 101031898 ID=MsG0680035247.01.T01;Parent=MsG0680035247.01;Name=MsG0680035247.01.T01;_AED=0.14;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|179
Chr6 exon 101032419 101032584 101032419 ID=MsG0680035247.01.T01:exon:10547;Parent=MsG0680035247.01.T01
Chr6 exon 101031898 101032271 101031898 ID=MsG0680035247.01.T01:exon:10546;Parent=MsG0680035247.01.T01
Chr6 CDS 101032419 101032584 101032419 ID=MsG0680035247.01.T01:cds;Parent=MsG0680035247.01.T01
Chr6 CDS 101031898 101032271 101031898 ID=MsG0680035247.01.T01:cds;Parent=MsG0680035247.01.T01
Gene Sequence

>MsG0680035247.01.T01

ATGGCATTTTCTAATGCTTTGGTTTTAACCTTCTTTTTAGCGATCACTATGGCACTGCCAACTTTTGCAACTGTCCACACCGTTGGAGATACTTCAGGTTGGACAATCGGTTCTGATTATAGCACATGGGCTAATGACAAAACCTTTGTAGTTGGAGACAGTCTTGTGTTCAATTATGGAATTGGGCACACGGTGGATGAAGTTCAAGAAAGTGACTATAAATCATGCATAACTAGAAATTCAATTAGTACAGACAGTAGCGGTGCCACAACCATTCCCTTCAAAAAAGCAGGAACGCATTACTTCATATGCGCTGTTCCTGGACATTGCGTATGTGGCATGAAACTCTCTGTTAAGGTTAACGCTTCTTCTACTTCTGCTGCTCCTTCTGCAACACCATCAGGGAAAGTTGATGGCACCCTTGCCCAACAACTACCACACATTCCACGACCACCACCACTCCTACCACGCAAAGTGCAACTTCAGCTACTAGTATCTCACCAATTTTTGCTTTCTTTTTCACAGTTTCATGGATCTTGA

Protein sequence

>MsG0680035247.01.T01

MAFSNALVLTFFLAITMALPTFATVHTVGDTSGWTIGSDYSTWANDKTFVVGDSLVFNYGIGHTVDEVQESDYKSCITRNSISTDSSGATTIPFKKAGTHYFICAVPGHCVCGMKLSVKVNASSTSAAPSATPSGKVDGTLAQQLPHIPRPPPLLPRKVQLQLLVSHQFLLSFSQFHGS*