Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035491.01.T01 | XP_024642386.1 | 80.556 | 108 | 21 | 0 | 3 | 110 | 655 | 762 | 2.22E-52 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035491.01.T01 | A0A2Z6NW35 | 66.038 | 106 | 36 | 0 | 5 | 110 | 290 | 395 | 2.93e-43 | 154 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680035491.01 | MsG0780041116.01 | PPI |
| MsG0680035491.01 | MsG0280010449.01 | PPI |
| MsG0680035491.01 | MsG0380013578.01 | PPI |
| MsG0680035491.01 | MsG0780040274.01 | PPI |
| MsG0680035491.01 | MsG0280009322.01 | PPI |
| MsG0680035491.01 | MsG0780041116.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035491.01.T01 | MTR_4g036855 | 59.259 | 108 | 16 | 1 | 3 | 110 | 12 | 91 | 1.50e-35 | 117 |
| MsG0680035491.01.T01 | MTR_7g063280 | 59.722 | 72 | 29 | 0 | 3 | 74 | 103 | 174 | 1.52e-26 | 97.1 |
| MsG0680035491.01.T01 | MTR_1g045560 | 51.250 | 80 | 23 | 1 | 3 | 82 | 26 | 89 | 2.82e-19 | 76.3 |
| MsG0680035491.01.T01 | MTR_3g465840 | 50.769 | 65 | 32 | 0 | 3 | 67 | 6 | 70 | 1.99e-18 | 75.5 |
| MsG0680035491.01.T01 | MTR_5g083510 | 41.558 | 77 | 45 | 0 | 34 | 110 | 16 | 92 | 4.34e-16 | 67.8 |
| MsG0680035491.01.T01 | MTR_5g031620 | 40.476 | 84 | 50 | 0 | 27 | 110 | 139 | 222 | 1.34e-15 | 69.7 |
| MsG0680035491.01.T01 | MTR_8g028785 | 44.156 | 77 | 43 | 0 | 3 | 79 | 289 | 365 | 3.96e-15 | 69.7 |
| MsG0680035491.01.T01 | MTR_2g027020 | 36.937 | 111 | 58 | 3 | 3 | 109 | 59 | 161 | 5.12e-15 | 67.4 |
| MsG0680035491.01.T01 | MTR_6g016265 | 50.000 | 56 | 28 | 0 | 3 | 58 | 70 | 125 | 1.26e-14 | 65.1 |
| MsG0680035491.01.T01 | MTR_5g075610 | 42.105 | 76 | 42 | 1 | 1 | 76 | 24 | 97 | 2.58e-14 | 63.5 |
| MsG0680035491.01.T01 | MTR_7g072030 | 65.854 | 41 | 14 | 0 | 3 | 43 | 80 | 120 | 7.73e-14 | 63.2 |
| MsG0680035491.01.T01 | MTR_3g010950 | 38.272 | 81 | 50 | 0 | 30 | 110 | 54 | 134 | 1.24e-13 | 62.8 |
| MsG0680035491.01.T01 | MTR_1g081930 | 47.368 | 57 | 30 | 0 | 3 | 59 | 69 | 125 | 2.18e-13 | 62.0 |
| MsG0680035491.01.T01 | MTR_4g064590 | 35.238 | 105 | 64 | 1 | 6 | 110 | 62 | 162 | 9.18e-13 | 61.2 |
| MsG0680035491.01.T01 | MTR_1g088650 | 41.096 | 73 | 43 | 0 | 3 | 75 | 117 | 189 | 1.98e-12 | 60.8 |
| MsG0680035491.01.T01 | MTR_1g057740 | 41.250 | 80 | 47 | 0 | 29 | 108 | 60 | 139 | 2.26e-12 | 59.7 |
| MsG0680035491.01.T01 | MTR_5g049680 | 58.696 | 46 | 18 | 1 | 3 | 47 | 62 | 107 | 8.77e-12 | 57.4 |
| MsG0680035491.01.T01 | MTR_6g034915 | 35.849 | 106 | 48 | 3 | 3 | 107 | 86 | 172 | 9.21e-12 | 58.9 |
| MsG0680035491.01.T01 | MTR_8g098925 | 37.313 | 67 | 42 | 0 | 3 | 69 | 96 | 162 | 1.27e-11 | 58.2 |
| MsG0680035491.01.T01 | MTR_2g088140 | 46.552 | 58 | 31 | 0 | 33 | 90 | 49 | 106 | 1.48e-11 | 57.0 |
| MsG0680035491.01.T01 | MTR_5g014420 | 55.814 | 43 | 19 | 0 | 9 | 51 | 72 | 114 | 1.74e-11 | 57.0 |
| MsG0680035491.01.T01 | MTR_8g088220 | 41.818 | 55 | 32 | 0 | 3 | 57 | 25 | 79 | 2.37e-11 | 55.5 |
| MsG0680035491.01.T01 | MTR_7g006380 | 44.776 | 67 | 36 | 1 | 28 | 94 | 95 | 160 | 3.62e-11 | 57.4 |
| MsG0680035491.01.T01 | MTR_4g098670 | 35.802 | 81 | 52 | 0 | 27 | 107 | 16 | 96 | 7.61e-11 | 54.7 |
| MsG0680035491.01.T01 | MTR_7g013520 | 32.407 | 108 | 48 | 1 | 3 | 110 | 101 | 183 | 9.50e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCCGCCTTTGCTAACTTA+TGG | 0.234398 | 6:-106735307 | None:intergenic |
| AATATAATCTTGCTTATTAG+AGG | 0.256597 | 6:-106735186 | None:intergenic |
| GGCCATATTGTCAGCTAGTT+TGG | 0.312640 | 6:+106735325 | MsG0680035491.01.T01:CDS |
| ATTTGTTATCGTCTCTTCAC+TGG | 0.329714 | 6:+106735096 | MsG0680035491.01.T01:CDS |
| AAGCAAGATTATATTAAATA+TGG | 0.330225 | 6:+106735194 | MsG0680035491.01.T01:CDS |
| GGCCGCCTTTGCTAACTTAT+GGG | 0.332297 | 6:-106735306 | None:intergenic |
| TTCATAATACCGACTAAATA+CGG | 0.391917 | 6:-106735240 | None:intergenic |
| GAGTCAAGTTCAAAATCTAT+TGG | 0.406653 | 6:-106735140 | None:intergenic |
| CTCCAAACTAGCTGACAATA+TGG | 0.406952 | 6:-106735327 | None:intergenic |
| TTCACTGGGTTCATGAATTG+AGG | 0.415162 | 6:+106735111 | MsG0680035491.01.T01:CDS |
| TGGGTTCATGAATTGAGGTT+AGG | 0.471248 | 6:+106735116 | MsG0680035491.01.T01:CDS |
| TTTGTTATCGTCTCTTCACT+GGG | 0.492341 | 6:+106735097 | MsG0680035491.01.T01:CDS |
| GATGCTTATTGAAGAAGCGT+TGG | 0.534793 | 6:+106735073 | None:intergenic |
| AATGTAAATCCGTATTTAGT+CGG | 0.540016 | 6:+106735231 | MsG0680035491.01.T01:CDS |
| CTTATTAGAGGCAAAGCTGT+CGG | 0.559885 | 6:-106735174 | None:intergenic |
| TGCCCATAAGTTAGCAAAGG+CGG | 0.603130 | 6:+106735304 | MsG0680035491.01.T01:CDS |
| GTGTGTTCAATACAATCAAG+TGG | 0.606050 | 6:-106735362 | None:intergenic |
| TGTGCGGAGACAATCAAATG+AGG | 0.613771 | 6:+106735280 | MsG0680035491.01.T01:CDS |
| ACTCTAGTGTTGAGTTTGTG+CGG | 0.621948 | 6:+106735264 | MsG0680035491.01.T01:CDS |
| GGTTGCCCATAAGTTAGCAA+AGG | 0.661005 | 6:+106735301 | MsG0680035491.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGCAAGATTATATTAAATA+TGG | + | Chr6:106735194-106735213 | MsG0680035491.01.T01:CDS | 15.0% |
| !! | AATATAATCTTGCTTATTAG+AGG | - | Chr6:106735189-106735208 | None:intergenic | 20.0% |
| ! | AATGTAAATCCGTATTTAGT+CGG | + | Chr6:106735231-106735250 | MsG0680035491.01.T01:CDS | 25.0% |
| !! | TTCATAATACCGACTAAATA+CGG | - | Chr6:106735243-106735262 | None:intergenic | 25.0% |
| GAGTCAAGTTCAAAATCTAT+TGG | - | Chr6:106735143-106735162 | None:intergenic | 30.0% | |
| GTGTGTTCAATACAATCAAG+TGG | - | Chr6:106735365-106735384 | None:intergenic | 35.0% | |
| ! | TTTGTTATCGTCTCTTCACT+GGG | + | Chr6:106735097-106735116 | MsG0680035491.01.T01:CDS | 35.0% |
| !! | ATTTGTTATCGTCTCTTCAC+TGG | + | Chr6:106735096-106735115 | MsG0680035491.01.T01:CDS | 35.0% |
| CTCCAAACTAGCTGACAATA+TGG | - | Chr6:106735330-106735349 | None:intergenic | 40.0% | |
| CTTATTAGAGGCAAAGCTGT+CGG | - | Chr6:106735177-106735196 | None:intergenic | 40.0% | |
| TGGGTTCATGAATTGAGGTT+AGG | + | Chr6:106735116-106735135 | MsG0680035491.01.T01:CDS | 40.0% | |
| TTCACTGGGTTCATGAATTG+AGG | + | Chr6:106735111-106735130 | MsG0680035491.01.T01:CDS | 40.0% | |
| ! | ACTCTAGTGTTGAGTTTGTG+CGG | + | Chr6:106735264-106735283 | MsG0680035491.01.T01:CDS | 40.0% |
| GGCCATATTGTCAGCTAGTT+TGG | + | Chr6:106735325-106735344 | MsG0680035491.01.T01:CDS | 45.0% | |
| GGTTGCCCATAAGTTAGCAA+AGG | + | Chr6:106735301-106735320 | MsG0680035491.01.T01:CDS | 45.0% | |
| TGCCCATAAGTTAGCAAAGG+CGG | + | Chr6:106735304-106735323 | MsG0680035491.01.T01:CDS | 45.0% | |
| TGTGCGGAGACAATCAAATG+AGG | + | Chr6:106735280-106735299 | MsG0680035491.01.T01:CDS | 45.0% | |
| GGCCGCCTTTGCTAACTTAT+GGG | - | Chr6:106735309-106735328 | None:intergenic | 50.0% | |
| TGGCCGCCTTTGCTAACTTA+TGG | - | Chr6:106735310-106735329 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 106735074 | 106735406 | 106735074 | ID=MsG0680035491.01;Name=MsG0680035491.01 |
| Chr6 | mRNA | 106735074 | 106735406 | 106735074 | ID=MsG0680035491.01.T01;Parent=MsG0680035491.01;Name=MsG0680035491.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|110 |
| Chr6 | exon | 106735074 | 106735406 | 106735074 | ID=MsG0680035491.01.T01:exon:21681;Parent=MsG0680035491.01.T01 |
| Chr6 | CDS | 106735074 | 106735406 | 106735074 | ID=MsG0680035491.01.T01:cds;Parent=MsG0680035491.01.T01 |
| Gene Sequence |
| Protein sequence |
