Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035595.01.T01 | QCH00495.1 | 46.528 | 288 | 143 | 5 | 4 | 282 | 18 | 303 | 4.20E-80 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035595.01.T01 | P15631 | 46.181 | 288 | 144 | 5 | 4 | 282 | 18 | 303 | 6.22E-83 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035595.01.T01 | A0A4D6TXV5 | 46.528 | 288 | 143 | 5 | 4 | 282 | 18 | 303 | 2.01e-80 | 254 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680035595.01 | MsG0680035596.01 | 0.823287 | 1.501926e-53 | 1.287684e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035595.01.T01 | MTR_2g088410 | 55.507 | 227 | 97 | 3 | 2 | 226 | 15 | 239 | 3.95e-83 | 251 |
| MsG0680035595.01.T01 | MTR_4g112550 | 55.556 | 225 | 97 | 2 | 3 | 226 | 17 | 239 | 4.86e-83 | 250 |
| MsG0680035595.01.T01 | MTR_4g020670 | 52.344 | 256 | 116 | 4 | 3 | 257 | 17 | 267 | 1.41e-82 | 252 |
| MsG0680035595.01.T01 | MTR_5g042120 | 54.032 | 248 | 107 | 5 | 2 | 247 | 15 | 257 | 2.05e-82 | 249 |
| MsG0680035595.01.T01 | MTR_5g053550 | 56.354 | 181 | 61 | 1 | 46 | 226 | 1 | 163 | 6.57e-67 | 206 |
| MsG0680035595.01.T01 | MTR_2g097460 | 47.347 | 245 | 93 | 4 | 2 | 245 | 15 | 224 | 3.25e-66 | 207 |
| MsG0680035595.01.T01 | MTR_3g032610 | 52.423 | 227 | 104 | 3 | 2 | 226 | 15 | 239 | 7.19e-66 | 206 |
| MsG0680035595.01.T01 | MTR_5g042130 | 56.219 | 201 | 84 | 3 | 2 | 200 | 15 | 213 | 1.11e-64 | 202 |
| MsG0680035595.01.T01 | MTR_5g028740 | 56.805 | 169 | 72 | 1 | 28 | 195 | 26 | 194 | 5.73e-63 | 197 |
| MsG0680035595.01.T01 | MTR_2g058960 | 55.556 | 180 | 76 | 1 | 47 | 226 | 1 | 176 | 7.10e-62 | 194 |
| MsG0680035595.01.T01 | MTR_5g019740 | 47.208 | 197 | 80 | 4 | 3 | 198 | 17 | 190 | 2.27e-54 | 175 |
| MsG0680035595.01.T01 | MTR_2g088420 | 47.208 | 197 | 80 | 4 | 3 | 198 | 17 | 190 | 6.22e-54 | 174 |
| MsG0680035595.01.T01 | MTR_5g053540 | 46.305 | 203 | 78 | 5 | 46 | 247 | 1 | 173 | 2.20e-50 | 164 |
| MsG0680035595.01.T01 | MTR_4g020660 | 44.388 | 196 | 70 | 4 | 3 | 198 | 17 | 173 | 2.87e-45 | 151 |
| MsG0680035595.01.T01 | MTR_4g112560 | 53.425 | 146 | 65 | 2 | 3 | 147 | 17 | 160 | 2.77e-44 | 148 |
| MsG0680035595.01.T01 | MTR_2g058950 | 40.092 | 217 | 74 | 6 | 2 | 217 | 15 | 176 | 2.56e-39 | 135 |
| MsG0680035595.01.T01 | MTR_2g077620 | 37.156 | 218 | 134 | 3 | 4 | 218 | 25 | 242 | 9.40e-39 | 138 |
| MsG0680035595.01.T01 | MTR_3g463840 | 52.713 | 129 | 57 | 3 | 2 | 128 | 15 | 141 | 3.19e-36 | 127 |
| MsG0680035595.01.T01 | MTR_5g065110 | 36.548 | 197 | 123 | 2 | 24 | 218 | 4 | 200 | 5.09e-36 | 131 |
| MsG0680035595.01.T01 | MTR_4g045797 | 51.128 | 133 | 62 | 2 | 3 | 134 | 17 | 147 | 9.67e-36 | 126 |
| MsG0680035595.01.T01 | MTR_7g029190 | 53.333 | 120 | 54 | 1 | 3 | 122 | 17 | 134 | 1.16e-33 | 124 |
| MsG0680035595.01.T01 | MTR_4g080270 | 55.357 | 112 | 47 | 2 | 2 | 112 | 15 | 124 | 1.10e-32 | 117 |
| MsG0680035595.01.T01 | MTR_8g080750 | 48.819 | 127 | 62 | 2 | 3 | 128 | 17 | 141 | 4.91e-31 | 117 |
| MsG0680035595.01.T01 | MTR_2g081910 | 34.197 | 193 | 104 | 2 | 24 | 215 | 4 | 174 | 7.65e-28 | 105 |
| MsG0680035595.01.T01 | MTR_1g030240 | 52.564 | 78 | 32 | 2 | 171 | 247 | 1 | 74 | 3.20e-19 | 80.5 |
| MsG0680035595.01.T01 | MTR_4g080280 | 50.667 | 75 | 36 | 1 | 171 | 245 | 1 | 74 | 5.64e-18 | 77.0 |
| MsG0680035595.01.T01 | MTR_2g011030 | 53.165 | 79 | 34 | 2 | 2 | 79 | 15 | 91 | 1.82e-17 | 76.3 |
| MsG0680035595.01.T01 | MTR_5g054370 | 47.126 | 87 | 41 | 3 | 2 | 87 | 15 | 97 | 1.22e-16 | 76.3 |
| MsG0680035595.01.T01 | MTR_5g054370 | 52.632 | 76 | 33 | 2 | 174 | 249 | 105 | 177 | 2.17e-16 | 75.9 |
| MsG0680035595.01.T01 | MTR_2g011040 | 48.193 | 83 | 40 | 1 | 146 | 225 | 1 | 83 | 2.74e-16 | 75.9 |
| MsG0680035595.01.T01 | MTR_0025s0030 | 44.444 | 90 | 46 | 3 | 2 | 90 | 15 | 101 | 3.57e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTGTTCATTATCAATTA+AGG | 0.226441 | 6:+108914438 | None:intergenic |
| GGGCAAATAGTTCATCTATT+TGG | 0.235478 | 6:+108914020 | None:intergenic |
| AGAGAAAACATACTAAAATA+TGG | 0.320976 | 6:-108914520 | MsG0680035595.01.T01:CDS |
| GGTGAGGGAAATTTAAAGTA+TGG | 0.339396 | 6:-108914244 | MsG0680035595.01.T01:CDS |
| CATTACAGTATAGAATTTAG+TGG | 0.363552 | 6:-108914055 | MsG0680035595.01.T01:CDS |
| ATACTGAAAGGATGGTTTCC+TGG | 0.376424 | 6:+108914112 | None:intergenic |
| CTATCTAATGAAGTTCTTGC+TGG | 0.415225 | 6:+108913878 | None:intergenic |
| CTTTAAATTTCCCTCACCTT+GGG | 0.421915 | 6:+108914249 | None:intergenic |
| TTTGACATAAATCTACAGAT+AGG | 0.423295 | 6:-108914211 | MsG0680035595.01.T01:CDS |
| GTTCTTGCTGGTAAGAACTT+TGG | 0.448655 | 6:+108913890 | None:intergenic |
| GATCCGTCTGTTGATTCCAT+TGG | 0.466557 | 6:+108914472 | None:intergenic |
| ATTAGTAGATCATATAGAAA+AGG | 0.498517 | 6:-108914617 | MsG0680035595.01.T01:CDS |
| CATACTAAAATATGGAATGA+TGG | 0.500323 | 6:-108914512 | MsG0680035595.01.T01:CDS |
| ACTTTAAATTTCCCTCACCT+TGG | 0.504599 | 6:+108914248 | None:intergenic |
| TATAACTTATACTGAAAGGA+TGG | 0.527058 | 6:+108914104 | None:intergenic |
| CTAAAATATGGAATGATGGT+AGG | 0.547662 | 6:-108914508 | MsG0680035595.01.T01:CDS |
| AGGAGAAGTTGCTATACCAA+TGG | 0.584896 | 6:-108914488 | MsG0680035595.01.T01:CDS |
| TTCACTAATAACCCAAGGTG+AGG | 0.594570 | 6:-108914260 | MsG0680035595.01.T01:CDS |
| AACTTCTTATCGAAGATGCT+AGG | 0.601246 | 6:-108914297 | MsG0680035595.01.T01:CDS |
| GATCAAACTCTCATGAGACC+AGG | 0.631135 | 6:-108914130 | MsG0680035595.01.T01:CDS |
| TCACTAATAACCCAAGGTGA+GGG | 0.631738 | 6:-108914259 | MsG0680035595.01.T01:CDS |
| AAACATTCACTAATAACCCA+AGG | 0.638581 | 6:-108914265 | MsG0680035595.01.T01:CDS |
| ATACCAATGGAATCAACAGA+CGG | 0.675695 | 6:-108914475 | MsG0680035595.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTACTTTTTATGATTATT+TGG | + | Chr6:108914086-108914105 | None:intergenic | 15.0% |
| !!! | AATTTGTTCATTATCAATTA+AGG | + | Chr6:108913995-108914014 | None:intergenic | 15.0% |
| !!! | ATAAAAAGTACTTTTATGAA+AGG | - | Chr6:108914093-108914112 | MsG0680035595.01.T01:CDS | 15.0% |
| !!! | ATTTTATAACTTATACTGAA+AGG | + | Chr6:108914333-108914352 | None:intergenic | 15.0% |
| !!! | TTTTGTTTAAAATTTTCTTC+AGG | + | Chr6:108913856-108913875 | None:intergenic | 15.0% |
| !! | AGAGAAAACATACTAAAATA+TGG | - | Chr6:108913910-108913929 | MsG0680035595.01.T01:CDS | 20.0% |
| !! | ATTAGTAGATCATATAGAAA+AGG | - | Chr6:108913813-108913832 | MsG0680035595.01.T01:CDS | 20.0% |
| !!! | AAAATTTTCTTCAGGTTTTA+AGG | + | Chr6:108913848-108913867 | None:intergenic | 20.0% |
| !!! | ATATTTTAGTATGTTTTCTC+TGG | + | Chr6:108913911-108913930 | None:intergenic | 20.0% |
| !!! | CTTTTTAGTGATTTTAGAAT+TGG | + | Chr6:108914456-108914475 | None:intergenic | 20.0% |
| ! | CATACTAAAATATGGAATGA+TGG | - | Chr6:108913918-108913937 | MsG0680035595.01.T01:CDS | 25.0% |
| ! | CATTACAGTATAGAATTTAG+TGG | - | Chr6:108914375-108914394 | MsG0680035595.01.T01:CDS | 25.0% |
| ! | TATAACTTATACTGAAAGGA+TGG | + | Chr6:108914329-108914348 | None:intergenic | 25.0% |
| ! | TTAGAAAAAAGACAGAAGAT+AGG | - | Chr6:108914039-108914058 | MsG0680035595.01.T01:CDS | 25.0% |
| ! | TTTGACATAAATCTACAGAT+AGG | - | Chr6:108914219-108914238 | MsG0680035595.01.T01:CDS | 25.0% |
| !!! | GTGATTCTAGTTTTATTAGT+GGG | + | Chr6:108914434-108914453 | None:intergenic | 25.0% |
| !!! | TGATTCTAGTTTTATTAGTG+GGG | + | Chr6:108914433-108914452 | None:intergenic | 25.0% |
| AAACATTCACTAATAACCCA+AGG | - | Chr6:108914165-108914184 | MsG0680035595.01.T01:CDS | 30.0% | |
| CTAAAATATGGAATGATGGT+AGG | - | Chr6:108913922-108913941 | MsG0680035595.01.T01:CDS | 30.0% | |
| GAGATAAAAACTGAACTTTC+AGG | - | Chr6:108914609-108914628 | MsG0680035595.01.T01:CDS | 30.0% | |
| !!! | CTTTCTTCTTGTTTTAGAAG+AGG | + | Chr6:108914510-108914529 | None:intergenic | 30.0% |
| !!! | GGTGATTCTAGTTTTATTAG+TGG | + | Chr6:108914435-108914454 | None:intergenic | 30.0% |
| AACTTCTTATCGAAGATGCT+AGG | - | Chr6:108914133-108914152 | MsG0680035595.01.T01:CDS | 35.0% | |
| ACTTTAAATTTCCCTCACCT+TGG | + | Chr6:108914185-108914204 | None:intergenic | 35.0% | |
| ATACCAATGGAATCAACAGA+CGG | - | Chr6:108913955-108913974 | MsG0680035595.01.T01:CDS | 35.0% | |
| CTATCTAATGAAGTTCTTGC+TGG | + | Chr6:108914555-108914574 | None:intergenic | 35.0% | |
| CTTTAAATTTCCCTCACCTT+GGG | + | Chr6:108914184-108914203 | None:intergenic | 35.0% | |
| GGGCAAATAGTTCATCTATT+TGG | + | Chr6:108914413-108914432 | None:intergenic | 35.0% | |
| ! | GGTGAGGGAAATTTAAAGTA+TGG | - | Chr6:108914186-108914205 | MsG0680035595.01.T01:CDS | 35.0% |
| AGGAGAAGTTGCTATACCAA+TGG | - | Chr6:108913942-108913961 | MsG0680035595.01.T01:CDS | 40.0% | |
| ATACTGAAAGGATGGTTTCC+TGG | + | Chr6:108914321-108914340 | None:intergenic | 40.0% | |
| GTTCTTGCTGGTAAGAACTT+TGG | + | Chr6:108914543-108914562 | None:intergenic | 40.0% | |
| TCACTAATAACCCAAGGTGA+GGG | - | Chr6:108914171-108914190 | MsG0680035595.01.T01:CDS | 40.0% | |
| TTCACTAATAACCCAAGGTG+AGG | - | Chr6:108914170-108914189 | MsG0680035595.01.T01:CDS | 40.0% | |
| GATCAAACTCTCATGAGACC+AGG | - | Chr6:108914300-108914319 | MsG0680035595.01.T01:CDS | 45.0% | |
| !! | GATCCGTCTGTTGATTCCAT+TGG | + | Chr6:108913961-108913980 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 108913802 | 108914650 | 108913802 | ID=MsG0680035595.01;Name=MsG0680035595.01 |
| Chr6 | mRNA | 108913802 | 108914650 | 108913802 | ID=MsG0680035595.01.T01;Parent=MsG0680035595.01;Name=MsG0680035595.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|282 |
| Chr6 | exon | 108913802 | 108914650 | 108913802 | ID=MsG0680035595.01.T01:exon:8501;Parent=MsG0680035595.01.T01 |
| Chr6 | CDS | 108913802 | 108914650 | 108913802 | ID=MsG0680035595.01.T01:cds;Parent=MsG0680035595.01.T01 |
| Gene Sequence |
| Protein sequence |
