AlfalfaGEDB | MsG0680035871.01

M. sativa cultivar ZhongmuNo.1 / MsG0680035871.01

KEGG: K01489
GO: GO:0003824 GO:0004126 GO:0008270 GO:0009972
PFAM: PF08211

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035871.01.T01	RHN53169.1	94.737	76	4	0	1	76	120	195	3.92E-43	148

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035871.01.T01	O65896	64.789	71	23	1	1	71	230	298	1.72E-23	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035871.01.T01	A0A396HIM2	94.737	76	4	0	1	76	120	195	1.87e-43	148

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048988.01	MsG0680035871.01	0.899065	2.866010e-77	3.484623e-73

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035871.01.T01	MTR_6g092660	94.737	76	4	0	1	76	222	297	8.44e-46	148
MsG0680035871.01.T01	MTR_8g101570	57.746	71	27	1	1	71	219	286	1.02e-21	85.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035871.01.T01	AT2G19570	64.789	71	23	1	1	71	230	298	1.75e-24	93.2
MsG0680035871.01.T01	AT4G29610	54.545	77	33	1	1	77	219	293	4.43e-21	84.0
MsG0680035871.01.T01	AT4G29570	58.333	72	27	3	1	71	223	292	1.26e-16	72.0
MsG0680035871.01.T01	AT4G29620	51.389	72	33	1	1	72	241	310	1.40e-16	72.4
MsG0680035871.01.T01	AT4G29650	55.556	72	28	2	1	71	177	245	1.57e-16	71.2
MsG0680035871.01.T01	AT4G29600	53.425	73	29	3	1	71	227	296	9.00e-16	70.1
MsG0680035871.01.T01	AT4G29640	50.000	78	34	3	1	76	224	298	1.05e-13	64.3

Find 29 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTAGCATGGGACCGGTTC+AGG	0.312849	6:+113698809	MsG0680035871.01.T01:CDS
ATACGGCAAGGCTGTTTCTT+AGG	0.332593	6:+113698940	MsG0680035871.01.T01:CDS
GGAGACGGTGCGGCGGAGTA+TGG	0.354349	6:+113698861	MsG0680035871.01.T01:CDS
GGGACCGGTTCAGGCTGCTT+TGG	0.365571	6:+113698818	MsG0680035871.01.T01:CDS
AGAGGATGCGGTGGTGAAAC+AGG	0.378180	6:+113698914	MsG0680035871.01.T01:CDS
GCCACCAAAGCAGCCTGAAC+CGG	0.415674	6:-113698822	None:intergenic
ACCGGTTCAGGCTGCTTTGG+TGG	0.418994	6:+113698821	MsG0680035871.01.T01:CDS
TGCGGCGGAGTATGGAGAAT+TGG	0.433354	6:+113698869	MsG0680035871.01.T01:CDS
TTTATTGTCGGTGCCGGAGA+CGG	0.475580	6:+113698846	MsG0680035871.01.T01:CDS
AGAATTGGTGGAAGCGGTGT+TGG	0.478163	6:+113698884	MsG0680035871.01.T01:CDS
ATACTCCGCCGCACCGTCTC+CGG	0.496985	6:-113698859	None:intergenic
ATTGGTGGAAGCGGTGTTGG+TGG	0.506203	6:+113698887	MsG0680035871.01.T01:CDS
TGCAGCTTATAATCCTAGCA+TGG	0.518529	6:+113698797	MsG0680035871.01.T01:CDS
GCCTGAACCGGTCCCATGCT+AGG	0.541079	6:-113698810	None:intergenic
AGCGGTGTTGGTGGAGAAAG+AGG	0.561960	6:+113698896	MsG0680035871.01.T01:CDS
GGTGGTGAAACAGGAGGATA+CGG	0.564967	6:+113698923	MsG0680035871.01.T01:CDS
TTATAATCCTAGCATGGGAC+CGG	0.572239	6:+113698803	MsG0680035871.01.T01:CDS
GTATGGAGAATTGGTGGAAG+CGG	0.578039	6:+113698878	MsG0680035871.01.T01:CDS
GAAGCTACATTGAGGTGCGA+TGG	0.586638	6:-113698964	None:intergenic
GGCGGAGTATGGAGAATTGG+TGG	0.610347	6:+113698872	MsG0680035871.01.T01:CDS
GTTGGTGGAGAAAGAGGATG+CGG	0.610963	6:+113698902	MsG0680035871.01.T01:CDS
TGTCGGTGCCGGAGACGGTG+CGG	0.611623	6:+113698851	MsG0680035871.01.T01:CDS
GCAGCTTATAATCCTAGCAT+GGG	0.617961	6:+113698798	MsG0680035871.01.T01:CDS
CGGTGCCGGAGACGGTGCGG+CGG	0.633103	6:+113698854	MsG0680035871.01.T01:CDS
ACCACATTGAAGCTACATTG+AGG	0.663804	6:-113698972	None:intergenic
GGTGGAGAAAGAGGATGCGG+TGG	0.682018	6:+113698905	MsG0680035871.01.T01:CDS
GGATGCGGTGGTGAAACAGG+AGG	0.700486	6:+113698917	MsG0680035871.01.T01:CDS
ACCTCAATGTAGCTTCAATG+TGG	0.704261	6:+113698971	MsG0680035871.01.T01:CDS
TGAAACAGGAGGATACGGCA+AGG	0.721213	6:+113698928	MsG0680035871.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACCACATTGAAGCTACATTG+AGG	-	Chr6:113698975-113698994	None:intergenic	40.0%
	ACCTCAATGTAGCTTCAATG+TGG	+	Chr6:113698971-113698990	MsG0680035871.01.T01:CDS	40.0%
	GCAGCTTATAATCCTAGCAT+GGG	+	Chr6:113698798-113698817	MsG0680035871.01.T01:CDS	40.0%
	TGCAGCTTATAATCCTAGCA+TGG	+	Chr6:113698797-113698816	MsG0680035871.01.T01:CDS	40.0%
	TTATAATCCTAGCATGGGAC+CGG	+	Chr6:113698803-113698822	MsG0680035871.01.T01:CDS	40.0%
!!	GCTTTGGTGGCTTTTATTGT+CGG	+	Chr6:113698834-113698853	MsG0680035871.01.T01:CDS	40.0%
	GTATGGAGAATTGGTGGAAG+CGG	+	Chr6:113698878-113698897	MsG0680035871.01.T01:CDS	45.0%
!!	ATACGGCAAGGCTGTTTCTT+AGG	+	Chr6:113698940-113698959	MsG0680035871.01.T01:CDS	45.0%
	AGAATTGGTGGAAGCGGTGT+TGG	+	Chr6:113698884-113698903	MsG0680035871.01.T01:CDS	50.0%
	GAAGCTACATTGAGGTGCGA+TGG	-	Chr6:113698967-113698986	None:intergenic	50.0%
	GGTGGTGAAACAGGAGGATA+CGG	+	Chr6:113698923-113698942	MsG0680035871.01.T01:CDS	50.0%
	TGAAACAGGAGGATACGGCA+AGG	+	Chr6:113698928-113698947	MsG0680035871.01.T01:CDS	50.0%
!	GTGGCTTTTATTGTCGGTGC+CGG	+	Chr6:113698840-113698859	MsG0680035871.01.T01:CDS	50.0%
!	GTTGGTGGAGAAAGAGGATG+CGG	+	Chr6:113698902-113698921	MsG0680035871.01.T01:CDS	50.0%
!!	TTTATTGTCGGTGCCGGAGA+CGG	+	Chr6:113698846-113698865	MsG0680035871.01.T01:CDS	50.0%
	AGAGGATGCGGTGGTGAAAC+AGG	+	Chr6:113698914-113698933	MsG0680035871.01.T01:CDS	55.0%
	GGCGGAGTATGGAGAATTGG+TGG	+	Chr6:113698872-113698891	MsG0680035871.01.T01:CDS	55.0%
	TGCGGCGGAGTATGGAGAAT+TGG	+	Chr6:113698869-113698888	MsG0680035871.01.T01:CDS	55.0%
!	ATTGGTGGAAGCGGTGTTGG+TGG	+	Chr6:113698887-113698906	MsG0680035871.01.T01:CDS	55.0%
!!	AGCGGTGTTGGTGGAGAAAG+AGG	+	Chr6:113698896-113698915	MsG0680035871.01.T01:CDS	55.0%
	GCCACCAAAGCAGCCTGAAC+CGG	-	Chr6:113698825-113698844	None:intergenic	60.0%
	GGATGCGGTGGTGAAACAGG+AGG	+	Chr6:113698917-113698936	MsG0680035871.01.T01:CDS	60.0%
	GGTGGAGAAAGAGGATGCGG+TGG	+	Chr6:113698905-113698924	MsG0680035871.01.T01:CDS	60.0%
!	ACCGGTTCAGGCTGCTTTGG+TGG	+	Chr6:113698821-113698840	MsG0680035871.01.T01:CDS	60.0%
!	TCCTAGCATGGGACCGGTTC+AGG	+	Chr6:113698809-113698828	MsG0680035871.01.T01:CDS	60.0%
	ATACTCCGCCGCACCGTCTC+CGG	-	Chr6:113698862-113698881	None:intergenic	65.0%
	GCCTGAACCGGTCCCATGCT+AGG	-	Chr6:113698813-113698832	None:intergenic	65.0%
!	GGGACCGGTTCAGGCTGCTT+TGG	+	Chr6:113698818-113698837	MsG0680035871.01.T01:CDS	65.0%
	GGAGACGGTGCGGCGGAGTA+TGG	+	Chr6:113698861-113698880	MsG0680035871.01.T01:CDS	70.0%
!!	TGTCGGTGCCGGAGACGGTG+CGG	+	Chr6:113698851-113698870	MsG0680035871.01.T01:CDS	70.0%
!!	CGGTGCCGGAGACGGTGCGG+CGG	+	Chr6:113698854-113698873	MsG0680035871.01.T01:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	113698789	113699025	113698789	ID=MsG0680035871.01;Name=MsG0680035871.01
Chr6	mRNA	113698789	113699025	113698789	ID=MsG0680035871.01.T01;Parent=MsG0680035871.01;Name=MsG0680035871.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|78
Chr6	exon	113698789	113699025	113698789	ID=MsG0680035871.01.T01:exon:15721;Parent=MsG0680035871.01.T01
Chr6	CDS	113698789	113699025	113698789	ID=MsG0680035871.01.T01:cds;Parent=MsG0680035871.01.T01

Gene Sequence	>MsG0680035871.01.T01 ATGGAATCTGCAGCTTATAATCCTAGCATGGGACCGGTTCAGGCTGCTTTGGTGGCTTTTATTGTCGGTGCCGGAGACGGTGCGGCGGAGTATGGAGAATTGGTGGAAGCGGTGTTGGTGGAGAAAGAGGATGCGGTGGTGAAACAGGAGGATACGGCAAGGCTGTTTCTTAGGTCCATCGCACCTCAATGTAGCTTCAATGTGGTTCTTTGCACTTCTAATAATAAATCTAATTGA
Protein sequence	>MsG0680035871.01.T01 MESAAYNPSMGPVQAALVAFIVGAGDGAAEYGELVEAVLVEKEDAVVKQEDTARLFLRSIAPQCSFNVVLCTSNNKSN*