Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048988.01.T01 | RHN53169.1 | 94.737 | 76 | 4 | 0 | 1 | 76 | 120 | 195 | 3.92E-43 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048988.01.T01 | O65896 | 64.789 | 71 | 23 | 1 | 1 | 71 | 230 | 298 | 1.72E-23 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048988.01.T01 | A0A396HIM2 | 94.737 | 76 | 4 | 0 | 1 | 76 | 120 | 195 | 1.87e-43 | 148 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048988.01 | MsG0080049059.01 | 0.820689 | 6.005456e-53 | 4.784795e-50 |
| MsG0080048988.01 | MsG0180004761.01 | -0.823200 | 1.574663e-53 | 1.346726e-50 |
| MsG0080048988.01 | MsG0680030315.01 | 0.806208 | 9.199214e-50 | 4.964109e-47 |
| MsG0080048988.01 | MsG0680035871.01 | 0.899065 | 2.866010e-77 | 3.484623e-73 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048988.01.T01 | MTR_6g092660 | 94.737 | 76 | 4 | 0 | 1 | 76 | 222 | 297 | 8.44e-46 | 148 |
| MsG0080048988.01.T01 | MTR_8g101570 | 57.746 | 71 | 27 | 1 | 1 | 71 | 219 | 286 | 1.02e-21 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048988.01.T01 | AT2G19570 | 64.789 | 71 | 23 | 1 | 1 | 71 | 230 | 298 | 1.75e-24 | 93.2 |
| MsG0080048988.01.T01 | AT4G29610 | 54.545 | 77 | 33 | 1 | 1 | 77 | 219 | 293 | 4.43e-21 | 84.0 |
| MsG0080048988.01.T01 | AT4G29570 | 58.333 | 72 | 27 | 3 | 1 | 71 | 223 | 292 | 1.26e-16 | 72.0 |
| MsG0080048988.01.T01 | AT4G29620 | 51.389 | 72 | 33 | 1 | 1 | 72 | 241 | 310 | 1.40e-16 | 72.4 |
| MsG0080048988.01.T01 | AT4G29650 | 55.556 | 72 | 28 | 2 | 1 | 71 | 177 | 245 | 1.57e-16 | 71.2 |
| MsG0080048988.01.T01 | AT4G29600 | 53.425 | 73 | 29 | 3 | 1 | 71 | 227 | 296 | 9.00e-16 | 70.1 |
| MsG0080048988.01.T01 | AT4G29640 | 50.000 | 78 | 34 | 3 | 1 | 76 | 224 | 298 | 1.05e-13 | 64.3 |
Find 29 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAGCATGGGACCGGTTC+AGG | 0.312849 | contig588end:+23100 | MsG0080048988.01.T01:CDS |
| ATACGGCAAGGCTGTTTCTT+AGG | 0.332593 | contig588end:+23231 | MsG0080048988.01.T01:CDS |
| GGAGACGGTGCGGCGGAGTA+TGG | 0.354349 | contig588end:+23152 | MsG0080048988.01.T01:CDS |
| GGGACCGGTTCAGGCTGCTT+TGG | 0.365571 | contig588end:+23109 | MsG0080048988.01.T01:CDS |
| AGAGGATGCGGTGGTGAAAC+AGG | 0.378180 | contig588end:+23205 | MsG0080048988.01.T01:CDS |
| GCCACCAAAGCAGCCTGAAC+CGG | 0.415674 | contig588end:-23113 | MsG0080048988.01.T01:intergenic |
| ACCGGTTCAGGCTGCTTTGG+TGG | 0.418994 | contig588end:+23112 | MsG0080048988.01.T01:CDS |
| TGCGGCGGAGTATGGAGAAT+TGG | 0.433354 | contig588end:+23160 | MsG0080048988.01.T01:CDS |
| TTTATTGTCGGTGCCGGAGA+CGG | 0.475580 | contig588end:+23137 | MsG0080048988.01.T01:CDS |
| AGAATTGGTGGAAGCGGTGT+TGG | 0.478163 | contig588end:+23175 | MsG0080048988.01.T01:CDS |
| ATACTCCGCCGCACCGTCTC+CGG | 0.496985 | contig588end:-23150 | MsG0080048988.01.T01:intergenic |
| ATTGGTGGAAGCGGTGTTGG+TGG | 0.506203 | contig588end:+23178 | MsG0080048988.01.T01:CDS |
| TGCAGCTTATAATCCTAGCA+TGG | 0.518529 | contig588end:+23088 | MsG0080048988.01.T01:CDS |
| GCCTGAACCGGTCCCATGCT+AGG | 0.541079 | contig588end:-23101 | MsG0080048988.01.T01:intergenic |
| AGCGGTGTTGGTGGAGAAAG+AGG | 0.561960 | contig588end:+23187 | MsG0080048988.01.T01:CDS |
| GGTGGTGAAACAGGAGGATA+CGG | 0.564967 | contig588end:+23214 | MsG0080048988.01.T01:CDS |
| TTATAATCCTAGCATGGGAC+CGG | 0.572239 | contig588end:+23094 | MsG0080048988.01.T01:CDS |
| GTATGGAGAATTGGTGGAAG+CGG | 0.578039 | contig588end:+23169 | MsG0080048988.01.T01:CDS |
| GAAGCTACATTGAGGTGCGA+TGG | 0.586638 | contig588end:-23255 | MsG0080048988.01.T01:intergenic |
| GGCGGAGTATGGAGAATTGG+TGG | 0.610347 | contig588end:+23163 | MsG0080048988.01.T01:CDS |
| GTTGGTGGAGAAAGAGGATG+CGG | 0.610963 | contig588end:+23193 | MsG0080048988.01.T01:CDS |
| TGTCGGTGCCGGAGACGGTG+CGG | 0.611623 | contig588end:+23142 | MsG0080048988.01.T01:CDS |
| GCAGCTTATAATCCTAGCAT+GGG | 0.617961 | contig588end:+23089 | MsG0080048988.01.T01:CDS |
| CGGTGCCGGAGACGGTGCGG+CGG | 0.633103 | contig588end:+23145 | MsG0080048988.01.T01:CDS |
| ACCACATTGAAGCTACATTG+AGG | 0.663804 | contig588end:-23263 | MsG0080048988.01.T01:intergenic |
| GGTGGAGAAAGAGGATGCGG+TGG | 0.682018 | contig588end:+23196 | MsG0080048988.01.T01:CDS |
| GGATGCGGTGGTGAAACAGG+AGG | 0.700486 | contig588end:+23208 | MsG0080048988.01.T01:CDS |
| ACCTCAATGTAGCTTCAATG+TGG | 0.704261 | contig588end:+23262 | MsG0080048988.01.T01:CDS |
| TGAAACAGGAGGATACGGCA+AGG | 0.721213 | contig588end:+23219 | MsG0080048988.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCACATTGAAGCTACATTG+AGG | - | contig588end:23266-23285 | MsG0080048987.01.T01:intergenic | 40.0% | |
| ACCTCAATGTAGCTTCAATG+TGG | + | contig588end:23262-23281 | MsG0080048988.01.T01:CDS | 40.0% | |
| GCAGCTTATAATCCTAGCAT+GGG | + | contig588end:23089-23108 | MsG0080048988.01.T01:CDS | 40.0% | |
| TGCAGCTTATAATCCTAGCA+TGG | + | contig588end:23088-23107 | MsG0080048988.01.T01:CDS | 40.0% | |
| TTATAATCCTAGCATGGGAC+CGG | + | contig588end:23094-23113 | MsG0080048988.01.T01:CDS | 40.0% | |
| !! | GCTTTGGTGGCTTTTATTGT+CGG | + | contig588end:23125-23144 | MsG0080048988.01.T01:CDS | 40.0% |
| GTATGGAGAATTGGTGGAAG+CGG | + | contig588end:23169-23188 | MsG0080048988.01.T01:CDS | 45.0% | |
| !! | ATACGGCAAGGCTGTTTCTT+AGG | + | contig588end:23231-23250 | MsG0080048988.01.T01:CDS | 45.0% |
| AGAATTGGTGGAAGCGGTGT+TGG | + | contig588end:23175-23194 | MsG0080048988.01.T01:CDS | 50.0% | |
| GAAGCTACATTGAGGTGCGA+TGG | - | contig588end:23258-23277 | MsG0080048988.01.T01:intergenic | 50.0% | |
| GGTGGTGAAACAGGAGGATA+CGG | + | contig588end:23214-23233 | MsG0080048988.01.T01:CDS | 50.0% | |
| TGAAACAGGAGGATACGGCA+AGG | + | contig588end:23219-23238 | MsG0080048988.01.T01:CDS | 50.0% | |
| ! | GTGGCTTTTATTGTCGGTGC+CGG | + | contig588end:23131-23150 | MsG0080048988.01.T01:CDS | 50.0% |
| ! | GTTGGTGGAGAAAGAGGATG+CGG | + | contig588end:23193-23212 | MsG0080048988.01.T01:CDS | 50.0% |
| !! | TTTATTGTCGGTGCCGGAGA+CGG | + | contig588end:23137-23156 | MsG0080048988.01.T01:CDS | 50.0% |
| AGAGGATGCGGTGGTGAAAC+AGG | + | contig588end:23205-23224 | MsG0080048988.01.T01:CDS | 55.0% | |
| GGCGGAGTATGGAGAATTGG+TGG | + | contig588end:23163-23182 | MsG0080048988.01.T01:CDS | 55.0% | |
| TGCGGCGGAGTATGGAGAAT+TGG | + | contig588end:23160-23179 | MsG0080048988.01.T01:CDS | 55.0% | |
| ! | ATTGGTGGAAGCGGTGTTGG+TGG | + | contig588end:23178-23197 | MsG0080048988.01.T01:CDS | 55.0% |
| !! | AGCGGTGTTGGTGGAGAAAG+AGG | + | contig588end:23187-23206 | MsG0080048988.01.T01:CDS | 55.0% |
| GCCACCAAAGCAGCCTGAAC+CGG | - | contig588end:23116-23135 | MsG0080048988.01.T01:intergenic | 60.0% | |
| GGATGCGGTGGTGAAACAGG+AGG | + | contig588end:23208-23227 | MsG0080048988.01.T01:CDS | 60.0% | |
| GGTGGAGAAAGAGGATGCGG+TGG | + | contig588end:23196-23215 | MsG0080048988.01.T01:CDS | 60.0% | |
| ! | ACCGGTTCAGGCTGCTTTGG+TGG | + | contig588end:23112-23131 | MsG0080048988.01.T01:CDS | 60.0% |
| ! | TCCTAGCATGGGACCGGTTC+AGG | + | contig588end:23100-23119 | MsG0080048988.01.T01:CDS | 60.0% |
| ATACTCCGCCGCACCGTCTC+CGG | - | contig588end:23153-23172 | MsG0080048988.01.T01:intergenic | 65.0% | |
| GCCTGAACCGGTCCCATGCT+AGG | - | contig588end:23104-23123 | MsG0080048988.01.T01:intergenic | 65.0% | |
| ! | GGGACCGGTTCAGGCTGCTT+TGG | + | contig588end:23109-23128 | MsG0080048988.01.T01:CDS | 65.0% |
| GGAGACGGTGCGGCGGAGTA+TGG | + | contig588end:23152-23171 | MsG0080048988.01.T01:CDS | 70.0% | |
| !! | TGTCGGTGCCGGAGACGGTG+CGG | + | contig588end:23142-23161 | MsG0080048988.01.T01:CDS | 70.0% |
| !! | CGGTGCCGGAGACGGTGCGG+CGG | + | contig588end:23145-23164 | MsG0080048988.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig588end | gene | 23080 | 23316 | 23080 | ID=MsG0080048988.01;Name=MsG0080048988.01 |
| contig588end | mRNA | 23080 | 23316 | 23080 | ID=MsG0080048988.01.T01;Parent=MsG0080048988.01;Name=MsG0080048988.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|78 |
| contig588end | exon | 23080 | 23316 | 23080 | ID=MsG0080048988.01.T01:exon:15312;Parent=MsG0080048988.01.T01 |
| contig588end | CDS | 23080 | 23316 | 23080 | ID=MsG0080048988.01.T01:cds;Parent=MsG0080048988.01.T01 |
| Gene Sequence |
| Protein sequence |
