Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035922.01.T01 | XP_019452086.1 | 77.778 | 63 | 14 | 0 | 1 | 63 | 11 | 73 | 1.28E-27 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035922.01.T01 | A0A4P1RBU9 | 77.778 | 63 | 14 | 0 | 1 | 63 | 11 | 73 | 6.13e-28 | 104 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005334.01 | MsG0780035922.01 | 0.803548 | 3.306893e-49 | 1.666600e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035922.01.T01 | MTR_3g074700 | 90.476 | 63 | 6 | 0 | 1 | 63 | 11 | 73 | 4.34e-28 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035922.01.T01 | AT1G27435 | 66.176 | 68 | 19 | 1 | 1 | 64 | 14 | 81 | 5.84e-25 | 88.2 |
Find 17 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCTTGGCATTGGCTTTA+CGG | 0.292715 | 7:-96838 | None:intergenic |
| CTTCTTGGCATTGGCTTTAC+GGG | 0.299540 | 7:-96837 | None:intergenic |
| ATGATTGTTCAGGACTCTTA+TGG | 0.325737 | 7:+96792 | MsG0780035922.01.T01:CDS |
| AGCATTCTTATTAACATTGT+TGG | 0.342299 | 7:-97285 | None:intergenic |
| TACACGGTGAAGACGCTTCT+TGG | 0.379760 | 7:-96852 | None:intergenic |
| GGCATTGGCTTTACGGGTGG+TGG | 0.416569 | 7:-96831 | None:intergenic |
| GTGAAGAAGCATGATTGTTC+AGG | 0.418282 | 7:+96782 | MsG0780035922.01.T01:CDS |
| GTGAAGACGCTTCTTGGCAT+TGG | 0.426306 | 7:-96846 | None:intergenic |
| CTTATTAACATTGTTGGCAC+TGG | 0.467015 | 7:-97279 | None:intergenic |
| AGAAGCGTCTTCACCGTGTA+TGG | 0.487373 | 7:+96855 | MsG0780035922.01.T01:CDS |
| GCGTCTTCACCGTGTATGGC+AGG | 0.511325 | 7:+96859 | MsG0780035922.01.T01:CDS |
| GAAGAATTTGCTGAAGGAGA+TGG | 0.527408 | 7:-97254 | None:intergenic |
| GAAGAATGAAGCTGTCCTCA+AGG | 0.567341 | 7:+97220 | MsG0780035922.01.T01:intron |
| CTTGGCATTGGCTTTACGGG+TGG | 0.585417 | 7:-96834 | None:intergenic |
| GTTCAAATACCTGCCATACA+CGG | 0.634907 | 7:-96868 | None:intergenic |
| CTGGCAGAAGAATTTGCTGA+AGG | 0.641376 | 7:-97260 | None:intergenic |
| ATGGTTCAGAACAAGCCTTG+AGG | 0.719399 | 7:-97235 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAGTACATTTTTTCTTTT+TGG | + | Chr7:97198-97217 | MsG0780035922.01.T01:intron | 15.0% |
| !! | ATTCCTTGAAAAAATGATTA+AGG | + | Chr7:96979-96998 | MsG0780035922.01.T01:intron | 20.0% |
| !! | TTCTACGTTAGTAATTAAAT+AGG | + | Chr7:96950-96969 | MsG0780035922.01.T01:intron | 20.0% |
| ! | ACATGAGCAAAAAACAAAAA+AGG | - | Chr7:97129-97148 | None:intergenic | 25.0% |
| ! | AGCATTCTTATTAACATTGT+TGG | - | Chr7:97288-97307 | None:intergenic | 25.0% |
| !! | ATATGTGCTTGTTGAAATAA+AGG | - | Chr7:97048-97067 | None:intergenic | 25.0% |
| !!! | CATCCTTAATCATTTTTTCA+AGG | - | Chr7:96985-97004 | None:intergenic | 25.0% |
| ACAATAATTGTGACTGTCAA+TGG | + | Chr7:97168-97187 | MsG0780035922.01.T01:intron | 30.0% | |
| CAGAAGAATCTAAATTAGCA+GGG | - | Chr7:96906-96925 | None:intergenic | 30.0% | |
| AAACAAAAAAGGCAAGCAGT+CGG | - | Chr7:97118-97137 | None:intergenic | 35.0% | |
| ATGATTGTTCAGGACTCTTA+TGG | + | Chr7:96792-96811 | MsG0780035922.01.T01:CDS | 35.0% | |
| CTTATTAACATTGTTGGCAC+TGG | - | Chr7:97282-97301 | None:intergenic | 35.0% | |
| GCAGAAGAATCTAAATTAGC+AGG | - | Chr7:96907-96926 | None:intergenic | 35.0% | |
| TTTCAACAAGCACATATGCA+TGG | + | Chr7:97051-97070 | MsG0780035922.01.T01:intron | 35.0% | |
| ! | GCTTGTTGAAATAAAGGCAA+TGG | - | Chr7:97042-97061 | None:intergenic | 35.0% |
| AACAAAAAAGGCAAGCAGTC+GGG | - | Chr7:97117-97136 | None:intergenic | 40.0% | |
| AACAAGCACATATGCATGGT+TGG | + | Chr7:97055-97074 | MsG0780035922.01.T01:intron | 40.0% | |
| ACAAGCACATATGCATGGTT+GGG | + | Chr7:97056-97075 | MsG0780035922.01.T01:intron | 40.0% | |
| GTTCAAATACCTGCCATACA+CGG | - | Chr7:96871-96890 | None:intergenic | 40.0% | |
| ! | GTGAAGAAGCATGATTGTTC+AGG | + | Chr7:96782-96801 | MsG0780035922.01.T01:CDS | 40.0% |
| !! | GAAGAATTTGCTGAAGGAGA+TGG | - | Chr7:97257-97276 | None:intergenic | 40.0% |
| AGGCAATGGCTCATCAACAA+CGG | - | Chr7:97028-97047 | None:intergenic | 45.0% | |
| ATGGTTCAGAACAAGCCTTG+AGG | - | Chr7:97238-97257 | None:intergenic | 45.0% | |
| GAAGAATGAAGCTGTCCTCA+AGG | + | Chr7:97220-97239 | MsG0780035922.01.T01:intron | 45.0% | |
| !! | CTGGCAGAAGAATTTGCTGA+AGG | - | Chr7:97263-97282 | None:intergenic | 45.0% |
| !! | CTTCTTGGCATTGGCTTTAC+GGG | - | Chr7:96840-96859 | None:intergenic | 45.0% |
| !! | GCTTCTTGGCATTGGCTTTA+CGG | - | Chr7:96841-96860 | None:intergenic | 45.0% |
| AGTCGGGCTTGGGAATCATT+TGG | - | Chr7:97101-97120 | None:intergenic | 50.0% | |
| GTGAAGACGCTTCTTGGCAT+TGG | - | Chr7:96849-96868 | None:intergenic | 50.0% | |
| TACACGGTGAAGACGCTTCT+TGG | - | Chr7:96855-96874 | None:intergenic | 50.0% | |
| !! | AGAAGCGTCTTCACCGTGTA+TGG | + | Chr7:96855-96874 | MsG0780035922.01.T01:CDS | 50.0% |
| AAAAGGCAAGCAGTCGGGCT+TGG | - | Chr7:97112-97131 | None:intergenic | 55.0% | |
| AAAGGCAAGCAGTCGGGCTT+GGG | - | Chr7:97111-97130 | None:intergenic | 55.0% | |
| !! | CTTGGCATTGGCTTTACGGG+TGG | - | Chr7:96837-96856 | None:intergenic | 55.0% |
| GCGTCTTCACCGTGTATGGC+AGG | + | Chr7:96859-96878 | MsG0780035922.01.T01:CDS | 60.0% | |
| !! | GGCATTGGCTTTACGGGTGG+TGG | - | Chr7:96834-96853 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 96773 | 97310 | 96773 | ID=MsG0780035922.01;Name=MsG0780035922.01 |
| Chr7 | mRNA | 96773 | 97310 | 96773 | ID=MsG0780035922.01.T01;Parent=MsG0780035922.01;Name=MsG0780035922.01.T01;_AED=0.48;_eAED=0.55;_QI=0|0|0|1|0|0|2|0|64 |
| Chr7 | exon | 96773 | 96880 | 96773 | ID=MsG0780035922.01.T01:exon:2103;Parent=MsG0780035922.01.T01 |
| Chr7 | exon | 97224 | 97310 | 97224 | ID=MsG0780035922.01.T01:exon:2104;Parent=MsG0780035922.01.T01 |
| Chr7 | CDS | 96773 | 96880 | 96773 | ID=MsG0780035922.01.T01:cds;Parent=MsG0780035922.01.T01 |
| Chr7 | CDS | 97224 | 97310 | 97224 | ID=MsG0780035922.01.T01:cds;Parent=MsG0780035922.01.T01 |
| Gene Sequence |
| Protein sequence |
