AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005334.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005334.01.T01 MTR_1g097380 91.837 98 8 0 10 107 159 256 2.60e-59 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005334.01.T01 AT1G71790 73.469 98 26 0 10 107 159 256 5.94e-50 159

Find 40 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTACGCATGATACTTTAA+TGG 0.211781 1:-90473737 MsG0180005334.01.T01:three_prime_UTR
ATGGTGTCTTCTTCTTCTTC+TGG 0.242086 1:+90475514 None:intergenic
AAGATTACAAATAAAATAAA+AGG 0.280009 1:+90473697 None:intergenic
CTGTACTGGTTAACCGATAA+TGG 0.322331 1:+90475495 None:intergenic
TGCATATTACAGGTATACTT+TGG 0.347203 1:-90474062 MsG0180005334.01.T01:intron
GGGAAGGATGATTGAAGAAA+TGG 0.378396 1:-90474294 MsG0180005334.01.T01:CDS
CTTTACCTCTATTACATGTA+TGG 0.387843 1:+90475685 None:intergenic
TTTCACATGGATTGGTGGTT+CGG 0.390679 1:-90473867 MsG0180005334.01.T01:three_prime_UTR
GAAGAAGAAGACACCATTAT+CGG 0.423903 1:-90475508 MsG0180005334.01.T01:CDS
CTTTGGGAAGACAAGAGAAA+TGG 0.432004 1:-90474045 MsG0180005334.01.T01:CDS
TCGGGAACCTTCAGTCTGTC+TGG 0.445337 1:-90475440 MsG0180005334.01.T01:CDS
GCATATTACAGGTATACTTT+GGG 0.451989 1:-90474061 MsG0180005334.01.T01:intron
ACATGGATTGGTGGTTCGGT+TGG 0.461417 1:-90473863 MsG0180005334.01.T01:three_prime_UTR
GCTGAGGAACTCGCTTGATC+AGG 0.466199 1:-90474264 MsG0180005334.01.T01:intron
CTCATTTGCGCCACTTCAGA+CGG 0.466227 1:+90474005 None:intergenic
GTCTGGATCAATTAGACGTC+AGG 0.467500 1:-90475423 MsG0180005334.01.T01:intron
TGTGTTACAGTATTTGAAGT+AGG 0.474254 1:-90473893 MsG0180005334.01.T01:three_prime_UTR
TGTTATATTAGATGAGTATG+AGG 0.476440 1:-90474349 MsG0180005334.01.T01:intron
GACTACAAATAATGAGTCAT+CGG 0.487491 1:-90475459 MsG0180005334.01.T01:CDS
CAAAGTCAACATAACTGTAC+TGG 0.498763 1:+90475481 None:intergenic
TGGATAGCAATAGAGAATCA+TGG 0.503305 1:-90473780 MsG0180005334.01.T01:three_prime_UTR
GTAGGAAGTTTCACATGGAT+TGG 0.513980 1:-90473875 MsG0180005334.01.T01:three_prime_UTR
GGATGCCATACATGTAATAG+AGG 0.526858 1:-90475690 MsG0180005334.01.T01:intron
GTGCAATGCAATCAGCTCTC+GGG 0.527836 1:+90473978 None:intergenic
GGAAGTTTCACATGGATTGG+TGG 0.535825 1:-90473872 MsG0180005334.01.T01:three_prime_UTR
ACTACAAATAATGAGTCATC+GGG 0.543741 1:-90475458 MsG0180005334.01.T01:CDS
GGTGCAATGCAATCAGCTCT+CGG 0.549682 1:+90473977 None:intergenic
GGATAGCAATAGAGAATCAT+GGG 0.551747 1:-90473779 MsG0180005334.01.T01:three_prime_UTR
GACATCTTTGTAACATGGGA+AGG 0.552251 1:-90474310 MsG0180005334.01.T01:CDS
ACTGAGACCACCGTCTGAAG+TGG 0.552812 1:-90474015 MsG0180005334.01.T01:CDS
ATGAGGCTATCAGTTGCTGA+TGG 0.584016 1:-90474332 MsG0180005334.01.T01:CDS
GTATATTTGTCTCTGCCACA+TGG 0.584950 1:+90473956 None:intergenic
TAAACTGTTGTGTTCTGTAG+TGG 0.586489 1:-90473800 MsG0180005334.01.T01:three_prime_UTR
TTGAAGTAGGAAGTTTCACA+TGG 0.605818 1:-90473880 MsG0180005334.01.T01:three_prime_UTR
TGATGGACATCTTTGTAACA+TGG 0.618055 1:-90474315 MsG0180005334.01.T01:CDS
ATTTGCGCCACTTCAGACGG+TGG 0.620496 1:+90474008 None:intergenic
AAGAAATGGAGAGTAAGCTG+AGG 0.653835 1:-90474280 MsG0180005334.01.T01:CDS
AATTGATCCAGACAGACTGA+AGG 0.662056 1:+90475433 None:intergenic
GATGGACATCTTTGTAACAT+GGG 0.688546 1:-90474314 MsG0180005334.01.T01:CDS
GCTGATTGCATTGCACCATG+TGG 0.734262 1:-90473971 MsG0180005334.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTACAAATAAAATAAA+AGG + Chr1:90475644-90475663 None:intergenic 10.0%
!! TTATTTAATTAGGATTAATA+GGG + Chr1:90474505-90474524 None:intergenic 10.0%
!! GTTATTTAATTAGGATTAAT+AGG + Chr1:90474506-90474525 None:intergenic 15.0%
!! TACATAATAGTCATATATTA+AGG + Chr1:90474887-90474906 None:intergenic 15.0%
!! TATTTAATTAGGATTAATAG+GGG + Chr1:90474504-90474523 None:intergenic 15.0%
!!! TAATGCATTGTTATTTAATT+AGG + Chr1:90474515-90474534 None:intergenic 15.0%
!!! TTTGTAATCTTTACTTTTTT+TGG - Chr1:90475653-90475672 MsG0180005334.01.T01:intron 15.0%
!! ATAACAGGAAAAGATTATTT+AGG + Chr1:90474975-90474994 None:intergenic 20.0%
!! ATATACATTTATACGAAAGA+TGG - Chr1:90474385-90474404 MsG0180005334.01.T01:intron 20.0%
!! GATAATTAAGTATATTAGGT+GGG - Chr1:90474132-90474151 MsG0180005334.01.T01:intron 20.0%
!! GTCATATATTAAGGATTTAA+TGG + Chr1:90474878-90474897 None:intergenic 20.0%
!! GTGTGATAATTAAGTATATT+AGG - Chr1:90474128-90474147 MsG0180005334.01.T01:intron 20.0%
!! TCTTTAATGTTAATTTAGGT+GGG - Chr1:90473800-90473819 MsG0180005334.01.T01:three_prime_UTR 20.0%
!! TGATAATTAAGTATATTAGG+TGG - Chr1:90474131-90474150 MsG0180005334.01.T01:intron 20.0%
!! TTGCTCTTTAATGTTAATTT+AGG - Chr1:90473796-90473815 MsG0180005334.01.T01:three_prime_UTR 20.0%
!!! TTCCTCATTACAATTTTATT+TGG - Chr1:90474603-90474622 MsG0180005334.01.T01:intron 20.0%
! ATAAACTGACCCTTAATTAT+TGG + Chr1:90474830-90474849 None:intergenic 25.0%
! ATCAGAGTTTATTTATCCTT+AGG + Chr1:90474367-90474386 None:intergenic 25.0%
! ATGCTTCTTACCAATAATTA+AGG - Chr1:90474817-90474836 MsG0180005334.01.T01:intron 25.0%
! CTCTTTAATGTTAATTTAGG+TGG - Chr1:90473799-90473818 MsG0180005334.01.T01:three_prime_UTR 25.0%
! GAATCAATAAATATGATACC+AGG - Chr1:90474315-90474334 MsG0180005334.01.T01:CDS 25.0%
! GACCAAATAAAATTGTAATG+AGG + Chr1:90474608-90474627 None:intergenic 25.0%
! TAAATTCAACTGAAGGTTTA+AGG + Chr1:90474194-90474213 None:intergenic 25.0%
! TCATACTCATCTAATATAAC+AGG + Chr1:90474990-90475009 None:intergenic 25.0%
! TGCTTCTTACCAATAATTAA+GGG - Chr1:90474818-90474837 MsG0180005334.01.T01:intron 25.0%
! TGTATGTTATTGCATATTAC+AGG - Chr1:90475266-90475285 MsG0180005334.01.T01:intron 25.0%
! TGTTATATTAGATGAGTATG+AGG - Chr1:90474989-90475008 MsG0180005334.01.T01:intron 25.0%
! TTATTCTCCATTAATTGATC+TGG - Chr1:90474554-90474573 MsG0180005334.01.T01:intron 25.0%
! TTCACTAAATCCTAAATAAC+AGG - Chr1:90474258-90474277 MsG0180005334.01.T01:intron 25.0%
! TTGAAATGTCATAGTTATGA+AGG + Chr1:90475210-90475229 None:intergenic 25.0%
!! ACTTCATTGACTGAATTATA+TGG - Chr1:90474042-90474061 MsG0180005334.01.T01:CDS 25.0%
!! ATTCATTTTCTTTCTCAACA+AGG - Chr1:90474001-90474020 MsG0180005334.01.T01:CDS 25.0%
!! CACTCTTTAACTATGTATTT+TGG - Chr1:90475117-90475136 MsG0180005334.01.T01:intron 25.0%
!!! AAGTTAGTCTTAAGTGAAAT+AGG - Chr1:90474703-90474722 MsG0180005334.01.T01:intron 25.0%
!!! CACTTAAGACTAACTTTTTT+GGG + Chr1:90474699-90474718 None:intergenic 25.0%
!!! CTTTAACTATGTATTTTGGT+AGG - Chr1:90475121-90475140 MsG0180005334.01.T01:intron 25.0%
!!! TCACTTAAGACTAACTTTTT+TGG + Chr1:90474700-90474719 None:intergenic 25.0%
AAAGATTGGTGGAATATATG+TGG + Chr1:90474084-90474103 None:intergenic 30.0%
AACCTTCAGTTGAATTTAGA+AGG - Chr1:90474196-90474215 MsG0180005334.01.T01:intron 30.0%
ACCTTCAGTTGAATTTAGAA+GGG - Chr1:90474197-90474216 MsG0180005334.01.T01:intron 30.0%
ACTACAAATAATGAGTCATC+GGG - Chr1:90473880-90473899 MsG0180005334.01.T01:three_prime_UTR 30.0%
ATATTCCACCAATCTTTAGA+AGG - Chr1:90474087-90474106 MsG0180005334.01.T01:intron 30.0%
ATCAGGTACTTCTTGATTAT+AGG - Chr1:90475091-90475110 MsG0180005334.01.T01:intron 30.0%
ATTAGGATTAATAGGGGTTA+CGG + Chr1:90474498-90474517 None:intergenic 30.0%
CATTACGCATGATACTTTAA+TGG - Chr1:90475601-90475620 MsG0180005334.01.T01:intron 30.0%
CTTTACCTCTATTACATGTA+TGG + Chr1:90473656-90473675 None:intergenic 30.0%
GACTACAAATAATGAGTCAT+CGG - Chr1:90473879-90473898 MsG0180005334.01.T01:three_prime_UTR 30.0%
GCATATTACAGGTATACTTT+GGG - Chr1:90475277-90475296 MsG0180005334.01.T01:intron 30.0%
GTAAACAGATGAAATTCCTA+AGG - Chr1:90474348-90474367 MsG0180005334.01.T01:intron 30.0%
TGAAATAGGCATCTATCATT+CGG - Chr1:90474717-90474736 MsG0180005334.01.T01:intron 30.0%
TGCATATTACAGGTATACTT+TGG - Chr1:90475276-90475295 MsG0180005334.01.T01:intron 30.0%
! AGTGTTTTCCAGTTGATTAA+AGG + Chr1:90473728-90473747 None:intergenic 30.0%
! ATGGATATAGCCTGTTATTT+AGG + Chr1:90474271-90474290 None:intergenic 30.0%
! CTGTTTACATTTTCAAATCC+TGG + Chr1:90474336-90474355 None:intergenic 30.0%
! TGTGTTACAGTATTTGAAGT+AGG - Chr1:90475445-90475464 MsG0180005334.01.T01:CDS 30.0%
! TTTACAGTCCTTCTAAAGAT+TGG + Chr1:90474098-90474117 None:intergenic 30.0%
!!! ATCACATGTTTTAGCATACT+GGG - Chr1:90475414-90475433 MsG0180005334.01.T01:intron 30.0%
!!! GGTACTTATTTTACATCTCT+CGG + Chr1:90474678-90474697 None:intergenic 30.0%
AGTAGCTTGAAGAATTATCG+AGG - Chr1:90474651-90474670 MsG0180005334.01.T01:intron 35.0%
CAAAGTCAACATAACTGTAC+TGG + Chr1:90473860-90473879 None:intergenic 35.0%
CACTTCTCCAGATCAATTAA+TGG + Chr1:90474564-90474583 None:intergenic 35.0%
CTATGACATTTCAAACTCGA+AGG - Chr1:90475216-90475235 MsG0180005334.01.T01:intron 35.0%
GAAATAGGCATCTATCATTC+GGG - Chr1:90474718-90474737 MsG0180005334.01.T01:intron 35.0%
GAAGAAGAAGACACCATTAT+CGG - Chr1:90473830-90473849 MsG0180005334.01.T01:three_prime_UTR 35.0%
GATGGACATCTTTGTAACAT+GGG - Chr1:90475024-90475043 MsG0180005334.01.T01:intron 35.0%
GGATAGCAATAGAGAATCAT+GGG - Chr1:90475559-90475578 MsG0180005334.01.T01:intron 35.0%
TAAACTGTTGTGTTCTGTAG+TGG - Chr1:90475538-90475557 MsG0180005334.01.T01:intron 35.0%
TAGAGATCAGATTGTCTTCT+TGG - Chr1:90475159-90475178 MsG0180005334.01.T01:intron 35.0%
TCCCTTCTAAATTCAACTGA+AGG + Chr1:90474201-90474220 None:intergenic 35.0%
TGGATAGCAATAGAGAATCA+TGG - Chr1:90475558-90475577 MsG0180005334.01.T01:intron 35.0%
TTAAGGCTGACAAGTGTTTA+GGG + Chr1:90474177-90474196 None:intergenic 35.0%
TTAGGATTAATAGGGGTTAC+GGG + Chr1:90474497-90474516 None:intergenic 35.0%
TTGAAGTAGGAAGTTTCACA+TGG - Chr1:90475458-90475477 MsG0180005334.01.T01:CDS 35.0%
! TGATGGACATCTTTGTAACA+TGG - Chr1:90475023-90475042 MsG0180005334.01.T01:intron 35.0%
! TTGTGAAGTCAGAAAGCATA+TGG + Chr1:90474290-90474309 None:intergenic 35.0%
! TTTAAGGCTGACAAGTGTTT+AGG + Chr1:90474178-90474197 None:intergenic 35.0%
!! ATTAAGTTGACACAAGCAAG+GGG + Chr1:90474931-90474950 None:intergenic 35.0%
!! CATTAAGTTGACACAAGCAA+GGG + Chr1:90474932-90474951 None:intergenic 35.0%
!!! CATCACATGTTTTAGCATAC+TGG - Chr1:90475413-90475432 MsG0180005334.01.T01:intron 35.0%
!!! TGTTGCTTTTTATCAACCCA+TGG + Chr1:90473696-90473715 None:intergenic 35.0%
AAGAAATGGAGAGTAAGCTG+AGG - Chr1:90475058-90475077 MsG0180005334.01.T01:intron 40.0%
AATTGATCCAGACAGACTGA+AGG + Chr1:90473908-90473927 None:intergenic 40.0%
ATGGTGTCTTCTTCTTCTTC+TGG + Chr1:90473827-90473846 None:intergenic 40.0%
CACAGCAACCTTTAATCAAC+TGG - Chr1:90473717-90473736 MsG0180005334.01.T01:three_prime_UTR 40.0%
GACATCTTTGTAACATGGGA+AGG - Chr1:90475028-90475047 MsG0180005334.01.T01:intron 40.0%
GGATGCCATACATGTAATAG+AGG - Chr1:90473648-90473667 MsG0180005334.01.T01:three_prime_UTR 40.0%
GGGAAGGATGATTGAAGAAA+TGG - Chr1:90475044-90475063 MsG0180005334.01.T01:intron 40.0%
GTAGGAAGTTTCACATGGAT+TGG - Chr1:90475463-90475482 MsG0180005334.01.T01:CDS 40.0%
GTATATTTGTCTCTGCCACA+TGG + Chr1:90475385-90475404 None:intergenic 40.0%
TAAGGCTGACAAGTGTTTAG+GGG + Chr1:90474176-90474195 None:intergenic 40.0%
! ACAGTCCTTCTAAAGATTGG+TGG + Chr1:90474095-90474114 None:intergenic 40.0%
! ACCCATGGTCTTGAAAACAA+AGG + Chr1:90473681-90473700 None:intergenic 40.0%
! CTTTGGGAAGACAAGAGAAA+TGG - Chr1:90475293-90475312 MsG0180005334.01.T01:intron 40.0%
! TCTGGAGAAGTGTGAACATT+AGG - Chr1:90474572-90474591 MsG0180005334.01.T01:intron 40.0%
!! CTGTACTGGTTAACCGATAA+TGG + Chr1:90473846-90473865 None:intergenic 40.0%
!! GCATTAAGTTGACACAAGCA+AGG + Chr1:90474933-90474952 None:intergenic 40.0%
!! TTTCACATGGATTGGTGGTT+CGG - Chr1:90475471-90475490 MsG0180005334.01.T01:CDS 40.0%
!!! ACATGTTTTAGCATACTGGG+TGG - Chr1:90475417-90475436 MsG0180005334.01.T01:intron 40.0%
!!! ATGTATTTTGGTAGGAGAGC+TGG - Chr1:90475129-90475148 MsG0180005334.01.T01:intron 40.0%
!!! GCCTTTGTTTTCAAGACCAT+GGG - Chr1:90473677-90473696 MsG0180005334.01.T01:three_prime_UTR 40.0%
!!! GGTTGGTCTTTGTTTTACTG+AGG - Chr1:90475492-90475511 MsG0180005334.01.T01:CDS 40.0%
!!! TGCCTTTGTTTTCAAGACCA+TGG - Chr1:90473676-90473695 MsG0180005334.01.T01:three_prime_UTR 40.0%
!!! TGTATTTTGGTAGGAGAGCT+GGG - Chr1:90475130-90475149 MsG0180005334.01.T01:intron 40.0%
AGAACAATCAGAGAACACGC+TGG - Chr1:90474778-90474797 MsG0180005334.01.T01:intron 45.0%
ATGAGGCTATCAGTTGCTGA+TGG - Chr1:90475006-90475025 MsG0180005334.01.T01:intron 45.0%
GTCTGGATCAATTAGACGTC+AGG - Chr1:90473915-90473934 MsG0180005334.01.T01:three_prime_UTR 45.0%
!! GGAAGTTTCACATGGATTGG+TGG - Chr1:90475466-90475485 MsG0180005334.01.T01:CDS 45.0%
CTCATTTGCGCCACTTCAGA+CGG + Chr1:90475336-90475355 None:intergenic 50.0%
GCTGATTGCATTGCACCATG+TGG - Chr1:90475367-90475386 MsG0180005334.01.T01:intron 50.0%
GGTGCAATGCAATCAGCTCT+CGG + Chr1:90475364-90475383 None:intergenic 50.0%
GTGCAATGCAATCAGCTCTC+GGG + Chr1:90475363-90475382 None:intergenic 50.0%
TTTGGTAGGAGAGCTGGGTT+GGG - Chr1:90475135-90475154 MsG0180005334.01.T01:intron 50.0%
! TTTTGGTAGGAGAGCTGGGT+TGG - Chr1:90475134-90475153 MsG0180005334.01.T01:intron 50.0%
!! ACATGGATTGGTGGTTCGGT+TGG - Chr1:90475475-90475494 MsG0180005334.01.T01:CDS 50.0%
ACTGAGACCACCGTCTGAAG+TGG - Chr1:90475323-90475342 MsG0180005334.01.T01:intron 55.0%
ATTTGCGCCACTTCAGACGG+TGG + Chr1:90475333-90475352 None:intergenic 55.0%
GCTGAGGAACTCGCTTGATC+AGG - Chr1:90475074-90475093 MsG0180005334.01.T01:intron 55.0%
TCGGGAACCTTCAGTCTGTC+TGG - Chr1:90473898-90473917 MsG0180005334.01.T01:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 90473645 90475715 90473645 ID=MsG0180005334.01;Name=MsG0180005334.01
Chr1 mRNA 90473645 90475715 90473645 ID=MsG0180005334.01.T01;Parent=MsG0180005334.01;Name=MsG0180005334.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0.6|0.83|1|1|1|6|344|107
Chr1 exon 90473645 90474072 90473645 ID=MsG0180005334.01.T01:exon:48051;Parent=MsG0180005334.01.T01
Chr1 exon 90474265 90474360 90474265 ID=MsG0180005334.01.T01:exon:48050;Parent=MsG0180005334.01.T01
Chr1 exon 90475424 90475542 90475424 ID=MsG0180005334.01.T01:exon:48049;Parent=MsG0180005334.01.T01
Chr1 exon 90475691 90475715 90475691 ID=MsG0180005334.01.T01:exon:48048;Parent=MsG0180005334.01.T01
Chr1 CDS 90475691 90475715 90475691 ID=MsG0180005334.01.T01:cds;Parent=MsG0180005334.01.T01
Chr1 CDS 90475424 90475542 90475424 ID=MsG0180005334.01.T01:cds;Parent=MsG0180005334.01.T01
Chr1 CDS 90474265 90474360 90474265 ID=MsG0180005334.01.T01:cds;Parent=MsG0180005334.01.T01
Chr1 CDS 90473989 90474072 90473989 ID=MsG0180005334.01.T01:cds;Parent=MsG0180005334.01.T01
Chr1 three_prime_UTR 90473645 90473988 90473645 ID=MsG0180005334.01.T01:three_prime_utr;Parent=MsG0180005334.01.T01
Gene Sequence

>MsG0180005334.01.T01

ATGGGATGCCATACATGTAATAGAGGTGGGACCAGAAGAAGAAGAAGACACCATTATCGGTTAACCAGTACAGTTATGTTGACTTTGACTACAAATAATGAGTCATCGGGAACCTTCAGTCTGTCTGGATCAATTAGACGTCAGATGAGTATGAGGCTATCAGTTGCTGATGGACATCTTTGTAACATGGGAAGGATGATTGAAGAAATGGAGAGTAAGCTGAGGAACTCGCTTGATCAGGTATACTTTGGGAAGACAAGAGAAATGGTTTGCACACTGAGACCACCGTCTGAAGTGGCGCAAATGAGAATGCCCGAGAGCTGA

Protein sequence

>MsG0180005334.01.T01

MGCHTCNRGGTRRRRRHHYRLTSTVMLTLTTNNESSGTFSLSGSIRRQMSMRLSVADGHLCNMGRMIEEMESKLRNSLDQVYFGKTREMVCTLRPPSEVAQMRMPES*