AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780035990.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035990.01.T01 MTR_7g006590 87.395 119 15 0 200 318 43 161 2.27e-70 217
MsG0780035990.01.T01 MTR_7g025610 38.571 280 158 5 1 275 39 309 2.10e-45 163
MsG0780035990.01.T01 MTR_7g007270 55.556 81 24 1 207 287 2 70 5.55e-21 88.6
MsG0780035990.01.T01 MTR_1g035250 24.454 229 153 5 53 276 162 375 4.31e-17 82.4
MsG0780035990.01.T01 MTR_8g054410 24.312 218 152 4 61 274 203 411 5.34e-15 76.3
MsG0780035990.01.T01 MTR_1g080110 23.239 284 182 7 28 280 71 349 3.75e-14 73.6
MsG0780035990.01.T01 MTR_6g077540 20.849 259 172 8 68 314 82 319 7.65e-14 72.4
MsG0780035990.01.T01 MTR_2g035450 21.254 287 200 7 8 279 104 379 1.07e-13 72.0
MsG0780035990.01.T01 MTR_7g118240 26.000 250 163 7 45 287 162 396 3.18e-13 70.5
MsG0780035990.01.T01 MTR_1g033960 20.141 283 173 5 65 310 294 560 5.02e-13 70.1
MsG0780035990.01.T01 MTR_7g067460 22.667 300 184 6 68 321 197 494 5.36e-13 70.1
MsG0780035990.01.T01 MTR_6g471100 24.000 225 152 4 68 285 206 418 5.80e-13 70.1
MsG0780035990.01.T01 MTR_2g049740 24.434 221 152 4 55 269 455 666 6.64e-13 70.1
MsG0780035990.01.T01 MTR_0014s0180 22.300 287 205 6 36 318 238 510 7.73e-13 69.3
MsG0780035990.01.T01 MTR_1g068960 24.113 282 178 9 11 280 80 337 1.18e-12 68.9
MsG0780035990.01.T01 MTR_1g068960 21.833 371 224 10 2 320 103 459 1.78e-11 65.5
MsG0780035990.01.T01 MTR_1g068960 23.507 268 166 8 36 269 274 536 3.15e-11 64.7
MsG0780035990.01.T01 MTR_2g018550 22.559 297 207 9 2 291 412 692 1.42e-12 68.9
MsG0780035990.01.T01 MTR_6g478210 22.358 246 165 7 55 286 63 296 1.54e-12 68.2
MsG0780035990.01.T01 MTR_1g011520 22.026 227 162 5 65 287 249 464 1.55e-12 68.6
MsG0780035990.01.T01 MTR_1g095880 22.727 220 147 6 59 269 235 440 2.04e-12 68.6
MsG0780035990.01.T01 MTR_1g095880 24.138 290 168 12 66 324 169 437 2.93e-11 64.7
MsG0780035990.01.T01 MTR_5g024320 24.413 213 152 2 59 271 201 404 2.30e-12 68.2
MsG0780035990.01.T01 MTR_8g105170 23.404 282 179 7 65 324 237 503 2.58e-12 68.2
MsG0780035990.01.T01 MTR_8g105170 21.053 228 163 4 58 279 440 656 6.81e-12 67.0
MsG0780035990.01.T01 MTR_1g050495 21.631 282 203 7 36 313 235 502 2.73e-12 67.8
MsG0780035990.01.T01 MTR_6g079890 22.358 246 165 7 55 286 199 432 2.90e-12 67.8
MsG0780035990.01.T01 MTR_3g054110 20.217 277 192 5 65 323 137 402 3.45e-12 67.4
MsG0780035990.01.T01 MTR_7g070420 23.288 292 170 8 59 309 279 557 3.50e-12 67.8
MsG0780035990.01.T01 MTR_5g090170 21.538 260 173 4 61 291 226 483 4.39e-12 67.4
MsG0780035990.01.T01 MTR_5g090170 21.645 231 164 4 61 285 433 652 6.14e-12 67.0
MsG0780035990.01.T01 MTR_5g090170 24.186 215 153 4 66 279 301 506 5.25e-11 63.9
MsG0780035990.01.T01 MTR_6g080210 23.755 261 174 7 22 269 248 496 5.35e-12 67.0
MsG0780035990.01.T01 MTR_5g090170 21.538 260 173 4 61 291 226 483 5.68e-12 67.0
MsG0780035990.01.T01 MTR_5g090170 21.645 231 164 4 61 285 433 652 7.39e-12 66.6
MsG0780035990.01.T01 MTR_5g090170 24.186 215 153 4 66 279 301 506 6.53e-11 63.9
MsG0780035990.01.T01 MTR_7g066990 21.973 223 158 5 59 275 456 668 6.81e-12 66.6
MsG0780035990.01.T01 MTR_6g079590 22.776 281 179 6 65 313 302 576 1.12e-11 65.9
MsG0780035990.01.T01 MTR_6g079590 23.348 227 160 4 47 269 390 606 8.93e-11 63.2
MsG0780035990.01.T01 MTR_8g070060 21.429 238 173 4 36 269 383 610 1.43e-11 65.9
MsG0780035990.01.T01 MTR_3g105900 23.860 285 196 6 35 313 761 1030 1.46e-11 65.9
MsG0780035990.01.T01 MTR_3g105900 24.231 260 172 7 22 269 323 569 3.24e-11 64.7
MsG0780035990.01.T01 MTR_4g040410 25.000 312 192 10 31 311 195 495 1.57e-11 65.5
MsG0780035990.01.T01 MTR_7g451470 21.201 283 193 5 63 324 341 614 1.61e-11 65.9
MsG0780035990.01.T01 MTR_6g078210 20.370 270 170 5 55 287 233 494 1.66e-11 65.5
MsG0780035990.01.T01 MTR_4g108650 19.512 246 185 3 63 304 384 620 1.73e-11 65.5
MsG0780035990.01.T01 MTR_6g078190 23.581 229 141 4 64 260 206 432 2.36e-11 64.7
MsG0780035990.01.T01 MTR_5g029690 20.179 223 155 6 65 276 137 347 2.84e-11 64.7
MsG0780035990.01.T01 MTR_3g100600 20.402 348 235 9 2 314 134 474 3.19e-11 64.7
MsG0780035990.01.T01 MTR_6g065540 24.336 226 156 5 49 269 84 299 3.39e-11 64.3
MsG0780035990.01.T01 MTR_6g065540 22.109 294 189 8 23 279 264 554 3.88e-11 64.3
MsG0780035990.01.T01 MTR_0019s0210 21.405 299 195 7 49 313 215 507 3.87e-11 64.3
MsG0780035990.01.T01 MTR_6g080200 20.623 257 180 6 68 313 241 484 4.39e-11 63.9
MsG0780035990.01.T01 MTR_2g041700 22.939 279 196 6 40 313 184 448 4.53e-11 63.9
MsG0780035990.01.T01 MTR_7g076550 23.913 230 153 6 65 285 135 351 4.91e-11 63.9
MsG0780035990.01.T01 MTR_6g068820 23.188 345 214 12 2 310 88 417 5.27e-11 63.5
MsG0780035990.01.T01 MTR_6g069300 21.569 255 148 4 63 313 302 508 6.14e-11 63.5
MsG0780035990.01.T01 MTR_6g079210 22.373 295 187 7 23 279 96 386 6.27e-11 63.2
MsG0780035990.01.T01 MTR_0110s0060 21.633 245 158 5 57 269 147 389 6.29e-11 63.5
MsG0780035990.01.T01 MTR_6g077800 24.444 225 156 5 55 275 248 462 7.40e-11 63.2
MsG0780035990.01.T01 MTR_6g078160 21.681 226 164 3 65 286 121 337 7.97e-11 63.2
MsG0780035990.01.T01 MTR_0014s0250 21.277 282 204 6 36 313 235 502 8.28e-11 63.2
MsG0780035990.01.T01 MTR_6g079830 24.434 221 153 5 64 280 114 324 9.74e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035990.01.T01 AT1G16830 29.242 277 178 6 1 273 78 340 3.34e-26 109
MsG0780035990.01.T01 AT1G79540 24.000 250 173 4 68 313 196 432 2.90e-18 86.3
MsG0780035990.01.T01 AT5G64320 22.082 317 180 6 61 324 243 545 8.57e-18 84.7
MsG0780035990.01.T01 AT5G64320 21.951 246 178 4 35 276 425 660 8.20e-14 72.8
MsG0780035990.01.T01 AT5G64320 22.830 311 211 8 10 312 368 657 5.56e-13 70.1
MsG0780035990.01.T01 AT1G03560 26.809 235 158 5 40 270 193 417 2.24e-17 83.2
MsG0780035990.01.T01 AT1G09900 27.490 251 153 7 40 282 143 372 2.41e-17 83.2
MsG0780035990.01.T01 AT1G09900 24.057 212 148 3 63 270 302 504 1.49e-13 71.6
MsG0780035990.01.T01 AT1G09900 25.630 238 157 5 40 270 210 434 6.72e-13 69.7
MsG0780035990.01.T01 AT1G62670 25.333 225 155 5 65 285 181 396 4.60e-15 76.3
MsG0780035990.01.T01 AT1G62670 25.333 225 155 5 65 285 181 396 4.60e-15 76.3
MsG0780035990.01.T01 AT3G53700 25.322 233 157 5 59 283 389 612 1.23e-14 75.1
MsG0780035990.01.T01 AT3G53700 21.831 284 196 6 5 279 93 359 1.34e-12 68.9
MsG0780035990.01.T01 AT3G53700 22.222 315 191 7 34 308 154 454 2.37e-12 68.2
MsG0780035990.01.T01 AT3G04760 21.849 238 177 4 39 271 91 324 1.72e-14 74.7
MsG0780035990.01.T01 AT3G04760 23.308 266 176 8 68 324 156 402 8.22e-12 66.2
MsG0780035990.01.T01 AT3G04760 21.145 227 163 4 50 269 245 462 9.09e-12 66.2
MsG0780035990.01.T01 AT3G22670 22.963 270 154 5 26 285 188 413 1.74e-14 74.3
MsG0780035990.01.T01 AT5G39710 23.228 254 173 6 40 285 211 450 7.02e-14 72.8
MsG0780035990.01.T01 AT5G39710 21.267 221 161 3 61 277 301 512 2.80e-11 65.1
MsG0780035990.01.T01 AT1G63130 22.967 209 148 3 65 269 181 380 9.80e-14 72.4
MsG0780035990.01.T01 AT1G63130 22.967 209 148 3 65 269 181 380 9.80e-14 72.4
MsG0780035990.01.T01 AT1G74580 21.285 249 183 4 63 307 384 623 1.00e-13 72.4
MsG0780035990.01.T01 AT1G74580 21.285 249 183 4 63 307 384 623 1.00e-13 72.4
MsG0780035990.01.T01 AT1G74580 21.285 249 183 4 63 307 384 623 1.00e-13 72.4
MsG0780035990.01.T01 AT5G18475 22.115 208 148 3 68 270 230 428 1.46e-13 71.6
MsG0780035990.01.T01 AT5G65560 25.316 237 144 4 65 270 353 587 4.18e-13 70.5
MsG0780035990.01.T01 AT5G65560 22.172 221 151 6 49 269 178 377 8.30e-11 63.5
MsG0780035990.01.T01 AT5G65560 25.316 237 144 4 65 270 353 587 4.18e-13 70.5
MsG0780035990.01.T01 AT5G65560 22.172 221 151 6 49 269 178 377 8.30e-11 63.5
MsG0780035990.01.T01 AT5G65560 25.316 237 144 4 65 270 353 587 4.18e-13 70.5
MsG0780035990.01.T01 AT5G65560 22.172 221 151 6 49 269 178 377 8.30e-11 63.5
MsG0780035990.01.T01 AT1G63150 24.201 219 153 4 65 279 183 392 4.22e-13 70.5
MsG0780035990.01.T01 AT1G63150 24.201 219 153 4 65 279 183 392 4.22e-13 70.5
MsG0780035990.01.T01 AT5G38730 24.818 274 184 8 4 269 102 361 1.07e-12 69.3
MsG0780035990.01.T01 AT3G04760 21.053 266 175 6 39 271 91 354 1.15e-12 68.9
MsG0780035990.01.T01 AT3G04760 21.145 227 163 4 50 269 275 492 8.84e-12 66.2
MsG0780035990.01.T01 AT3G04760 23.881 268 181 7 61 324 184 432 1.02e-11 66.2
MsG0780035990.01.T01 AT2G02150 21.667 240 150 5 64 269 291 526 1.40e-12 68.9
MsG0780035990.01.T01 AT2G02150 21.667 240 150 5 64 269 291 526 1.40e-12 68.9
MsG0780035990.01.T01 AT3G16710 23.026 304 184 8 65 322 183 482 1.90e-12 68.2
MsG0780035990.01.T01 AT5G46100 27.222 180 113 4 99 273 119 285 2.32e-12 67.8
MsG0780035990.01.T01 AT5G46100 20.784 255 187 6 29 278 116 360 4.01e-12 67.0
MsG0780035990.01.T01 AT1G09820 25.306 245 156 7 47 279 236 465 2.96e-12 67.8
MsG0780035990.01.T01 AT3G48810 24.766 214 142 5 65 273 176 375 3.11e-12 67.8
MsG0780035990.01.T01 AT1G64583 26.087 230 156 5 60 285 142 361 3.15e-12 67.4
MsG0780035990.01.T01 AT5G16640 21.695 295 202 7 49 324 163 447 3.42e-12 67.4
MsG0780035990.01.T01 AT3G16710 24.314 255 157 7 65 287 183 433 3.45e-12 67.4
MsG0780035990.01.T01 AT1G64583 26.087 230 156 5 60 285 167 386 3.52e-12 67.4
MsG0780035990.01.T01 AT1G11710 26.721 247 154 9 48 284 137 366 3.64e-12 67.4
MsG0780035990.01.T01 AT1G11710 26.721 247 154 9 48 284 166 395 4.08e-12 67.4
MsG0780035990.01.T01 AT1G62680 23.715 253 179 5 37 285 88 330 4.36e-12 67.4
MsG0780035990.01.T01 AT1G62680 23.715 253 179 5 37 285 88 330 4.36e-12 67.4
MsG0780035990.01.T01 AT5G16420 22.442 303 208 5 10 308 135 414 6.84e-12 66.6
MsG0780035990.01.T01 AT5G16420 19.808 313 230 6 5 312 93 389 9.65e-11 63.2
MsG0780035990.01.T01 AT5G16420 22.442 303 208 5 10 308 135 414 6.84e-12 66.6
MsG0780035990.01.T01 AT5G16420 19.808 313 230 6 5 312 93 389 9.65e-11 63.2
MsG0780035990.01.T01 AT5G16420 22.442 303 208 5 10 308 135 414 6.84e-12 66.6
MsG0780035990.01.T01 AT5G16420 19.808 313 230 6 5 312 93 389 9.65e-11 63.2
MsG0780035990.01.T01 AT5G16420 22.442 303 208 5 10 308 135 414 6.84e-12 66.6
MsG0780035990.01.T01 AT5G16420 19.808 313 230 6 5 312 93 389 9.65e-11 63.2
MsG0780035990.01.T01 AT4G20090 20.442 362 230 10 11 322 93 446 8.21e-12 66.6
MsG0780035990.01.T01 AT4G20090 20.442 362 230 10 11 322 93 446 8.21e-12 66.6
MsG0780035990.01.T01 AT1G05670 23.179 302 171 10 57 324 198 472 9.01e-12 66.6
MsG0780035990.01.T01 AT1G05670 21.941 237 161 5 57 284 409 630 3.14e-11 64.7
MsG0780035990.01.T01 AT1G05670 23.179 302 171 10 57 324 198 472 9.01e-12 66.6
MsG0780035990.01.T01 AT1G05670 21.941 237 161 5 57 284 409 630 3.14e-11 64.7
MsG0780035990.01.T01 AT1G05670 23.179 302 171 10 57 324 198 472 9.01e-12 66.6
MsG0780035990.01.T01 AT1G05670 21.941 237 161 5 57 284 409 630 3.14e-11 64.7
MsG0780035990.01.T01 AT4G01400 26.423 246 155 10 64 298 184 414 1.23e-11 65.9
MsG0780035990.01.T01 AT5G61400 24.664 223 150 5 59 275 329 539 1.83e-11 65.5
MsG0780035990.01.T01 AT1G62720 21.705 258 178 6 37 285 72 314 2.41e-11 64.7
MsG0780035990.01.T01 AT1G63630 21.888 233 160 5 65 290 56 273 4.28e-11 63.2
MsG0780035990.01.T01 AT1G63230 23.431 239 149 7 65 290 106 323 4.41e-11 63.5
MsG0780035990.01.T01 AT4G19890 23.034 178 128 2 99 276 391 559 4.62e-11 64.3
MsG0780035990.01.T01 AT1G12775 22.656 256 175 5 64 312 292 531 4.66e-11 64.3
MsG0780035990.01.T01 AT1G30290 23.697 211 146 5 65 269 307 508 4.86e-11 64.3
MsG0780035990.01.T01 AT1G30290 19.217 281 198 6 59 322 478 746 9.06e-11 63.5
MsG0780035990.01.T01 AT1G30290 23.697 211 146 5 65 269 307 508 5.19e-11 64.3
MsG0780035990.01.T01 AT1G30290 19.217 281 198 6 59 322 478 746 9.67e-11 63.2
MsG0780035990.01.T01 AT1G62910 23.923 209 146 4 65 269 183 382 5.85e-11 63.9
MsG0780035990.01.T01 AT1G11630 21.538 260 186 6 28 280 104 352 6.15e-11 63.5
MsG0780035990.01.T01 AT3G22470 22.744 277 182 7 47 312 223 478 6.46e-11 63.5
MsG0780035990.01.T01 AT5G55840 23.750 240 165 6 40 273 129 356 8.15e-11 63.5
MsG0780035990.01.T01 AT5G55840 23.750 240 165 6 40 273 169 396 9.15e-11 63.5
MsG0780035990.01.T01 AT5G55840 23.750 240 165 6 40 273 169 396 9.15e-11 63.5

Find 58 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCATAAATGTTGTGAATT+AGG 0.114569 7:-1001596 MsG0780035990.01.T01:CDS
ATTCCTTCGACTCATGTTTA+TGG 0.137273 7:-1001783 MsG0780035990.01.T01:CDS
ATATTTAAAGCTATTGTATT+TGG 0.182223 7:+1001947 None:intergenic
AGGTCAGTCATTATCATAAT+AGG 0.223587 7:+1001275 None:intergenic
GCCGCCAGTGTATCAACTTT+TGG 0.230074 7:-1001672 MsG0780035990.01.T01:CDS
AATAGCTTTAAATATTCTTA+TGG 0.237897 7:-1001939 MsG0780035990.01.T01:CDS
CTCTTAGACTTGAAATCTAT+TGG 0.263751 7:-1002097 MsG0780035990.01.T01:CDS
TTCATAAATGTTGTGAATTA+GGG 0.265182 7:-1001595 MsG0780035990.01.T01:CDS
TTTGCAAAACAATCTTTATC+TGG 0.292868 7:+1001407 None:intergenic
CATTCATACCAGAATTTATA+AGG 0.304846 7:+1001255 None:intergenic
GTAATGTCACCAGGATAATA+AGG 0.320956 7:+1001758 None:intergenic
TTGGGTCTAAAGGTTAAGTT+AGG 0.355688 7:-1001653 MsG0780035990.01.T01:CDS
ATGTTGTAAGAGTGAAAATC+AGG 0.377581 7:+1001854 None:intergenic
ATGACTGACCTTATAAATTC+TGG 0.382411 7:-1001263 MsG0780035990.01.T01:CDS
GGTCAGTCATTATCATAATA+GGG 0.409592 7:+1001276 None:intergenic
TCACCACCTTTGCGAAATAG+TGG 0.412717 7:-1001825 MsG0780035990.01.T01:CDS
CGAATGAGGCGATCTATAAC+GGG 0.424891 7:+1002142 None:intergenic
TAACACATCATCCACCTTGT+TGG 0.433464 7:+1001466 None:intergenic
ACGAATGAGGCGATCTATAA+CGG 0.438700 7:+1002141 None:intergenic
GCCAATGGATCTCTAAAAGT+TGG 0.442320 7:-1001206 MsG0780035990.01.T01:CDS
ATAGTCATTCAATTTACAAA+TGG 0.456959 7:+1001715 None:intergenic
TTTATGGTGCCTTATTATCC+TGG 0.457373 7:-1001767 MsG0780035990.01.T01:CDS
CACCACATGGTAAATTCTCA+TGG 0.457373 7:-1001977 MsG0780035990.01.T01:CDS
AATAAAACAGTATGTGTGAC+AGG 0.466572 7:+1001506 None:intergenic
GACAACACTAAATGAACAAT+CGG 0.467545 7:+1001529 None:intergenic
GTCAATGCCATGCTTTCTGA+AGG 0.475712 7:-1001437 MsG0780035990.01.T01:CDS
TCGAAGGAATTCTGAAAGAC+GGG 0.480201 7:+1001796 None:intergenic
TAATAGGGATTTGATTATCA+AGG 0.483139 7:+1001291 None:intergenic
GTAGCTGCTCTTTGTGCTGA+TGG 0.488816 7:-1001380 MsG0780035990.01.T01:CDS
GGTGGATGATGTGTTACATT+TGG 0.510706 7:-1001459 MsG0780035990.01.T01:CDS
GGAATTGAATTCTCTACCAA+TGG 0.511812 7:-1001632 MsG0780035990.01.T01:CDS
TTGAATTCTCTACCAATGGT+TGG 0.514469 7:-1001628 MsG0780035990.01.T01:CDS
CAATTGATTTGAAACGAATG+AGG 0.534268 7:+1002128 None:intergenic
TTGTGCTGATGGTAGAATTG+AGG 0.549954 7:-1001369 MsG0780035990.01.T01:CDS
GTCGAAGGAATTCTGAAAGA+CGG 0.558887 7:+1001795 None:intergenic
AATGATTGAAGTCCAACCAT+TGG 0.566324 7:+1001616 None:intergenic
TTCAAAACCTTCAGAAAGCA+TGG 0.566977 7:+1001430 None:intergenic
TGTTGTAAGAGTGAAAATCA+GGG 0.568093 7:+1001855 None:intergenic
TCAAGGTGAACATCACTATG+AGG 0.577525 7:+1001308 None:intergenic
GATCCACTATTTCGCAAAGG+TGG 0.591491 7:+1001822 None:intergenic
GCACCATAAACATGAGTCGA+AGG 0.593698 7:+1001780 None:intergenic
ACCACATGGTAAATTCTCAT+GGG 0.606171 7:-1001976 MsG0780035990.01.T01:CDS
AGACCCAAAAGTTGATACAC+TGG 0.622663 7:+1001668 None:intergenic
CGTCGATGCTTATCACCACA+TGG 0.625998 7:-1001990 MsG0780035990.01.T01:CDS
AATGAGGCGATCTATAACGG+GGG 0.630919 7:+1002144 None:intergenic
TAAAAGTTGGAGAACTCTAG+AGG 0.630983 7:-1001193 None:intergenic
GTTGTAAGAGTGAAAATCAG+GGG 0.654303 7:+1001856 None:intergenic
AGAGAACACTTATAGCAGCA+AGG 0.656650 7:+1001891 None:intergenic
TTGTTAAAAGTAATGTCACC+AGG 0.658642 7:+1001749 None:intergenic
CGAAGGAATTCTGAAAGACG+GGG 0.662969 7:+1001797 None:intergenic
GAAGATCCACTATTTCGCAA+AGG 0.666718 7:+1001819 None:intergenic
AAATTCTGGTATGAATGACA+AGG 0.673965 7:-1001249 MsG0780035990.01.T01:CDS
ATAAAACAGTATGTGTGACA+GGG 0.687138 7:+1001507 None:intergenic
CCCAAAAGTTGATACACTGG+CGG 0.690395 7:+1001671 None:intergenic
GAATGAGGCGATCTATAACG+GGG 0.721477 7:+1002143 None:intergenic
TTATCTGACAGGTAAACACG+AGG 0.726755 7:-1002017 MsG0780035990.01.T01:CDS
TCTGACAGGTAAACACGAGG+TGG 0.728129 7:-1002014 MsG0780035990.01.T01:CDS
ACCCATGAGAATTTACCATG+TGG 0.744106 7:+1001975 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGCTTTAAATATTCTTA+TGG - Chr7:1001405-1001424 MsG0780035990.01.T01:CDS 15.0%
!!! ATATTTAAAGCTATTGTATT+TGG + Chr7:1001400-1001419 None:intergenic 15.0%
!! ATAGTCATTCAATTTACAAA+TGG + Chr7:1001632-1001651 None:intergenic 20.0%
!! ATTCATAAATGTTGTGAATT+AGG - Chr7:1001748-1001767 MsG0780035990.01.T01:CDS 20.0%
!! TTCATAAATGTTGTGAATTA+GGG - Chr7:1001749-1001768 MsG0780035990.01.T01:CDS 20.0%
!!! TGTTTTATTTAAGGCATTTT+TGG - Chr7:1001852-1001871 MsG0780035990.01.T01:CDS 20.0%
! AATCATGTTGAAGAAAAAGT+TGG + Chr7:1001791-1001810 None:intergenic 25.0%
! CATTCATACCAGAATTTATA+AGG + Chr7:1002092-1002111 None:intergenic 25.0%
! TAATAGGGATTTGATTATCA+AGG + Chr7:1002056-1002075 None:intergenic 25.0%
! TTTGCAAAACAATCTTTATC+TGG + Chr7:1001940-1001959 None:intergenic 25.0%
!!! CACACATACTGTTTTATTTA+AGG - Chr7:1001843-1001862 MsG0780035990.01.T01:CDS 25.0%
!!! TTAAGACTTTTTTATCTGAC+AGG - Chr7:1001316-1001335 MsG0780035990.01.T01:CDS 25.0%
AAATTCTGGTATGAATGACA+AGG - Chr7:1002095-1002114 MsG0780035990.01.T01:CDS 30.0%
AATAAAACAGTATGTGTGAC+AGG + Chr7:1001841-1001860 None:intergenic 30.0%
AGGTCAGTCATTATCATAAT+AGG + Chr7:1002072-1002091 None:intergenic 30.0%
ATAAAACAGTATGTGTGACA+GGG + Chr7:1001840-1001859 None:intergenic 30.0%
ATGACTGACCTTATAAATTC+TGG - Chr7:1002081-1002100 MsG0780035990.01.T01:CDS 30.0%
ATGTTGTAAGAGTGAAAATC+AGG + Chr7:1001493-1001512 None:intergenic 30.0%
CTCTTAGACTTGAAATCTAT+TGG - Chr7:1001247-1001266 MsG0780035990.01.T01:CDS 30.0%
GACAACACTAAATGAACAAT+CGG + Chr7:1001818-1001837 None:intergenic 30.0%
GGTCAGTCATTATCATAATA+GGG + Chr7:1002071-1002090 None:intergenic 30.0%
TGTTGTAAGAGTGAAAATCA+GGG + Chr7:1001492-1001511 None:intergenic 30.0%
TTGTTAAAAGTAATGTCACC+AGG + Chr7:1001598-1001617 None:intergenic 30.0%
!! CAATTGATTTGAAACGAATG+AGG + Chr7:1001219-1001238 None:intergenic 30.0%
!! CATTACTTTTAACAAGGTTC+TGG - Chr7:1001603-1001622 MsG0780035990.01.T01:CDS 30.0%
!! TGGTGACATTACTTTTAACA+AGG - Chr7:1001597-1001616 MsG0780035990.01.T01:CDS 30.0%
AATGATTGAAGTCCAACCAT+TGG + Chr7:1001731-1001750 None:intergenic 35.0%
ACCACATGGTAAATTCTCAT+GGG - Chr7:1001368-1001387 MsG0780035990.01.T01:CDS 35.0%
ATTCCTTCGACTCATGTTTA+TGG - Chr7:1001561-1001580 MsG0780035990.01.T01:CDS 35.0%
ATTGGCTAAGGTAAAAACAG+TGG + Chr7:1002124-1002143 None:intergenic 35.0%
GGAATTGAATTCTCTACCAA+TGG - Chr7:1001712-1001731 MsG0780035990.01.T01:CDS 35.0%
GTAATGTCACCAGGATAATA+AGG + Chr7:1001589-1001608 None:intergenic 35.0%
GTTGTAAGAGTGAAAATCAG+GGG + Chr7:1001491-1001510 None:intergenic 35.0%
TTCAAAACCTTCAGAAAGCA+TGG + Chr7:1001917-1001936 None:intergenic 35.0%
TTGAATTCTCTACCAATGGT+TGG - Chr7:1001716-1001735 MsG0780035990.01.T01:CDS 35.0%
TTGGGTCTAAAGGTTAAGTT+AGG - Chr7:1001691-1001710 MsG0780035990.01.T01:CDS 35.0%
! TTTATGGTGCCTTATTATCC+TGG - Chr7:1001577-1001596 MsG0780035990.01.T01:CDS 35.0%
!! AGAAAAAGTTGGTAGCATCA+TGG + Chr7:1001780-1001799 None:intergenic 35.0%
!! ATTTTTGGATTCCAACAAGG+TGG - Chr7:1001867-1001886 MsG0780035990.01.T01:CDS 35.0%
!! GTATCAACTTTTGGGTCTAA+AGG - Chr7:1001681-1001700 MsG0780035990.01.T01:CDS 35.0%
!! TCCAACTTTTAGAGATCCAT+TGG + Chr7:1002142-1002161 None:intergenic 35.0%
!! TTTTAGAGATCCATTGGCTA+AGG + Chr7:1002136-1002155 None:intergenic 35.0%
!!! ACTGTTTTTACCTTAGCCAA+TGG - Chr7:1002123-1002142 MsG0780035990.01.T01:CDS 35.0%
ACCCATGAGAATTTACCATG+TGG + Chr7:1001372-1001391 None:intergenic 40.0%
ACGAATGAGGCGATCTATAA+CGG + Chr7:1001206-1001225 None:intergenic 40.0%
AGAGAACACTTATAGCAGCA+AGG + Chr7:1001456-1001475 None:intergenic 40.0%
CACCACATGGTAAATTCTCA+TGG - Chr7:1001367-1001386 MsG0780035990.01.T01:CDS 40.0%
GAAGATCCACTATTTCGCAA+AGG + Chr7:1001528-1001547 None:intergenic 40.0%
GGTGGATGATGTGTTACATT+TGG - Chr7:1001885-1001904 MsG0780035990.01.T01:CDS 40.0%
TAACACATCATCCACCTTGT+TGG + Chr7:1001881-1001900 None:intergenic 40.0%
TCAAGGTGAACATCACTATG+AGG + Chr7:1002039-1002058 None:intergenic 40.0%
TTATCTGACAGGTAAACACG+AGG - Chr7:1001327-1001346 MsG0780035990.01.T01:CDS 40.0%
! GCCAATGGATCTCTAAAAGT+TGG - Chr7:1002138-1002157 MsG0780035990.01.T01:CDS 40.0%
! GTCGAAGGAATTCTGAAAGA+CGG + Chr7:1001552-1001571 None:intergenic 40.0%
! TCGAAGGAATTCTGAAAGAC+GGG + Chr7:1001551-1001570 None:intergenic 40.0%
! TTGTGCTGATGGTAGAATTG+AGG - Chr7:1001975-1001994 MsG0780035990.01.T01:CDS 40.0%
!! AGACCCAAAAGTTGATACAC+TGG + Chr7:1001679-1001698 None:intergenic 40.0%
!! GGCATTTTTGGATTCCAACA+AGG - Chr7:1001864-1001883 MsG0780035990.01.T01:CDS 40.0%
AATGAGGCGATCTATAACGG+GGG + Chr7:1001203-1001222 None:intergenic 45.0%
CGAATGAGGCGATCTATAAC+GGG + Chr7:1001205-1001224 None:intergenic 45.0%
GAATGAGGCGATCTATAACG+GGG + Chr7:1001204-1001223 None:intergenic 45.0%
GATCCACTATTTCGCAAAGG+TGG + Chr7:1001525-1001544 None:intergenic 45.0%
GCACCATAAACATGAGTCGA+AGG + Chr7:1001567-1001586 None:intergenic 45.0%
GTCAATGCCATGCTTTCTGA+AGG - Chr7:1001907-1001926 MsG0780035990.01.T01:CDS 45.0%
TCACCACCTTTGCGAAATAG+TGG - Chr7:1001519-1001538 MsG0780035990.01.T01:CDS 45.0%
! CCGCCAGTGTATCAACTTTT+GGG - Chr7:1001673-1001692 MsG0780035990.01.T01:CDS 45.0%
! CGAAGGAATTCTGAAAGACG+GGG + Chr7:1001550-1001569 None:intergenic 45.0%
!! CCCAAAAGTTGATACACTGG+CGG + Chr7:1001676-1001695 None:intergenic 45.0%
CGTCGATGCTTATCACCACA+TGG - Chr7:1001354-1001373 MsG0780035990.01.T01:CDS 50.0%
GTAGCTGCTCTTTGTGCTGA+TGG - Chr7:1001964-1001983 MsG0780035990.01.T01:CDS 50.0%
TCTGACAGGTAAACACGAGG+TGG - Chr7:1001330-1001349 MsG0780035990.01.T01:CDS 50.0%
! GCCGCCAGTGTATCAACTTT+TGG - Chr7:1001672-1001691 MsG0780035990.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 1001196 1002170 1001196 ID=MsG0780035990.01;Name=MsG0780035990.01
Chr7 mRNA 1001196 1002170 1001196 ID=MsG0780035990.01.T01;Parent=MsG0780035990.01;Name=MsG0780035990.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|324
Chr7 exon 1001196 1002170 1001196 ID=MsG0780035990.01.T01:exon:15593;Parent=MsG0780035990.01.T01
Chr7 CDS 1001196 1002170 1001196 ID=MsG0780035990.01.T01:cds;Parent=MsG0780035990.01.T01
Gene Sequence

>MsG0780035990.01.T01

ATGGCCCCCGTTATAGATCGCCTCATTCGTTTCAAATCAATTGAGTCTATTCTCTTAGACTTGAAATCTATTGGTTTATTCAATGATTCAAACACAAAATCTCACTCTTTTTTACGTTTGTTAAGACTTTTTTATCTGACAGGTAAACACGAGGTGGTCGTCGATGCTTATCACCACATGGTAAATTCTCATGGGTTCGTTCCAAATACAATAGCTTTAAATATTCTTATGGATTCTCTGTTTTCTACTCATCAATACCTTGCTGCTATAAGTGTTCTCTTAGAAATGAATTCCCCTGATTTTCACTCTTACAACATTACACTTCACCACCTTTGCGAAATAGTGGATCTTCCCCGTCTTTCAGAATTCCTTCGACTCATGTTTATGGTGCCTTATTATCCTGGTGACATTACTTTTAACAAGGTTCTGGAATCCATTTGTAAATTGAATGACTATACTGTCATGATCGATGTCATGCCGCCAGTGTATCAACTTTTGGGTCTAAAGGTTAAGTTAGGAATTGAATTCTCTACCAATGGTTGGACTTCAATCATTCATAAATGTTGTGAATTAGGGAGACTCCATGATGCTACCAACTTTTTCTTCAACATGATTCAAACCGATTGTTCATTTAGTGTTGTCCCTGTCACACATACTGTTTTATTTAAGGCATTTTTGGATTCCAACAAGGTGGATGATGTGTTACATTTGGTCAATGCCATGCTTTCTGAAGGTTTTGAACCAGATAAAGATTGTTTTGCAAAACTAGTAGCTGCTCTTTGTGCTGATGGTAGAATTGAGGAAGCAGTTAGTGCGTATCGTGATATTGTTATGAAGCATCCTCATAGTGATGTTCACCTTGATAATCAAATCCCTATTATGATAATGACTGACCTTATAAATTCTGGTATGAATGACAAGGCTGCCACTGTTTTTACCTTAGCCAATGGATCTCTAAAAGTTGGAGAACTCTAG
Protein sequence

>MsG0780035990.01.T01

MAPVIDRLIRFKSIESILLDLKSIGLFNDSNTKSHSFLRLLRLFYLTGKHEVVVDAYHHMVNSHGFVPNTIALNILMDSLFSTHQYLAAISVLLEMNSPDFHSYNITLHHLCEIVDLPRLSEFLRLMFMVPYYPGDITFNKVLESICKLNDYTVMIDVMPPVYQLLGLKVKLGIEFSTNGWTSIIHKCCELGRLHDATNFFFNMIQTDCSFSVVPVTHTVLFKAFLDSNKVDDVLHLVNAMLSEGFEPDKDCFAKLVAALCADGRIEEAVSAYRDIVMKHPHSDVHLDNQIPIMIMTDLINSGMNDKAATVFTLANGSLKVGEL*