Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037590.01.T01 | RHN45205.1 | 71.92 | 349 | 92 | 3 | 1 | 347 | 1 | 345 | 1.31E-172 | 498 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037590.01.T01 | A0A396H261 | 71.920 | 349 | 92 | 3 | 1 | 347 | 1 | 345 | 6.26e-173 | 498 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048687.01 | MsG0780037590.01 | -0.802154 | 6.417313e-49 | 3.120884e-46 |
| MsG0080048971.01 | MsG0780037590.01 | -0.807677 | 4.497029e-50 | 2.520353e-47 |
| MsG0180000614.01 | MsG0780037590.01 | -0.819510 | 1.118455e-52 | 8.624315e-50 |
| MsG0180000743.01 | MsG0780037590.01 | -0.806617 | 7.541891e-50 | 4.112104e-47 |
| MsG0180003793.01 | MsG0780037590.01 | -0.842227 | 2.941879e-58 | 4.430624e-55 |
| MsG0180004854.01 | MsG0780037590.01 | -0.803442 | 3.480010e-49 | 1.748936e-46 |
| MsG0180004861.01 | MsG0780037590.01 | -0.803794 | 2.940506e-49 | 1.491265e-46 |
| MsG0280006993.01 | MsG0780037590.01 | -0.801522 | 8.652876e-49 | 4.141142e-46 |
| MsG0280007477.01 | MsG0780037590.01 | 0.828680 | 7.858509e-55 | 7.866013e-52 |
| MsG0280007847.01 | MsG0780037590.01 | -0.808263 | 3.375293e-50 | 1.921154e-47 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037590.01.T01 | MTR_7g033915 | 65.909 | 352 | 92 | 5 | 1 | 349 | 1 | 327 | 1.75e-156 | 446 |
| MsG0780037590.01.T01 | MTR_7g033990 | 64.306 | 353 | 114 | 7 | 1 | 349 | 1 | 345 | 1.16e-144 | 418 |
| MsG0780037590.01.T01 | MTR_7g034120 | 69.203 | 276 | 79 | 3 | 74 | 347 | 1 | 272 | 1.28e-131 | 381 |
| MsG0780037590.01.T01 | MTR_7g034110 | 62.356 | 348 | 86 | 5 | 1 | 348 | 1 | 303 | 2.47e-129 | 377 |
| MsG0780037590.01.T01 | MTR_1g041245 | 51.261 | 357 | 157 | 6 | 1 | 349 | 1 | 348 | 3.17e-107 | 322 |
| MsG0780037590.01.T01 | MTR_7g034115 | 48.257 | 373 | 171 | 7 | 1 | 364 | 1 | 360 | 1.95e-99 | 301 |
| MsG0780037590.01.T01 | MTR_7g032380 | 46.610 | 354 | 148 | 8 | 1 | 349 | 1 | 318 | 3.21e-91 | 280 |
| MsG0780037590.01.T01 | MTR_5g012840 | 47.319 | 317 | 119 | 3 | 34 | 349 | 12 | 281 | 1.02e-90 | 277 |
| MsG0780037590.01.T01 | MTR_7g033945 | 77.143 | 175 | 39 | 1 | 1 | 174 | 1 | 175 | 6.56e-89 | 267 |
| MsG0780037590.01.T01 | MTR_7g115270 | 41.969 | 386 | 192 | 9 | 4 | 366 | 5 | 381 | 8.97e-85 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037590.01.T01 | AT5G56370 | 32.216 | 388 | 199 | 18 | 10 | 348 | 1 | 373 | 3.30e-30 | 121 |
| MsG0780037590.01.T01 | AT5G56370 | 32.216 | 388 | 199 | 18 | 10 | 348 | 1 | 373 | 3.30e-30 | 121 |
| MsG0780037590.01.T01 | AT4G00160 | 30.000 | 400 | 212 | 18 | 5 | 348 | 10 | 397 | 9.34e-29 | 117 |
| MsG0780037590.01.T01 | AT5G56380 | 27.654 | 405 | 218 | 11 | 10 | 347 | 1 | 397 | 2.89e-26 | 110 |
| MsG0780037590.01.T01 | AT5G56380 | 27.654 | 405 | 218 | 11 | 10 | 347 | 1 | 397 | 2.89e-26 | 110 |
| MsG0780037590.01.T01 | AT4G10400 | 30.729 | 384 | 206 | 16 | 10 | 347 | 1 | 370 | 3.95e-26 | 109 |
| MsG0780037590.01.T01 | AT4G10400 | 30.729 | 384 | 206 | 16 | 10 | 347 | 1 | 370 | 3.95e-26 | 109 |
| MsG0780037590.01.T01 | AT4G10400 | 30.729 | 384 | 206 | 16 | 10 | 347 | 1 | 370 | 3.95e-26 | 109 |
| MsG0780037590.01.T01 | AT3G52680 | 29.529 | 403 | 208 | 18 | 5 | 348 | 15 | 400 | 1.01e-25 | 108 |
| MsG0780037590.01.T01 | AT4G26340 | 29.082 | 392 | 211 | 17 | 10 | 347 | 1 | 379 | 1.64e-25 | 107 |
Find 95 sgRNAs with CRISPR-Local
Find 348 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATAACCTTCTTGCGTA+TGG | 0.469095 | 7:+28953937 | MsG0780037590.01.T01:CDS |
| AAAGTTTCTGCGTCTGGACC+TGG | 0.516321 | 7:+28952399 | MsG0780037590.01.T01:CDS |
| AACAATCGGATGGGCAAGGT+AGG | 0.556566 | 7:-28951905 | None:intergenic |
| AACAGGGTGGTTAAGAAGAA+AGG | 0.446416 | 7:+28956586 | MsG0780037590.01.T01:intron |
| AACTAGAAGATGGATGCAAA+TGG | 0.494472 | 7:+28956665 | MsG0780037590.01.T01:CDS |
| AAGATAACCTTCTTGCGTAT+GGG | 0.554561 | 7:+28953938 | MsG0780037590.01.T01:CDS |
| AATATCATTTGGAACCCTTA+AGG | 0.335425 | 7:-28952284 | None:intergenic |
| AATGGCAAATGTCATCCAAA+TGG | 0.495605 | 7:-28952174 | None:intergenic |
| AATGTTAGCTCTAACCAAAT+TGG | 0.364294 | 7:-28952332 | None:intergenic |
| ACAAATTGATTGATATCATG+TGG | 0.586214 | 7:-28951962 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGTTGGACAAAGAATGTT+GGG | + | Chr7:28953517-28953536 | MsG0780037590.01.T01:intron | 30.0% | |
| AAATACATGCAATAGTAGCA+TGG | + | Chr7:28953064-28953083 | MsG0780037590.01.T01:intron | 30.0% | |
| AAATCTCATACACATATGAG+AGG | - | Chr7:28954712-28954731 | None:intergenic | 30.0% | |
| AAATTATCTTCATGTCCAAG+GGG | + | Chr7:28952971-28952990 | MsG0780037590.01.T01:intron | 30.0% | |
| AATACATGCAATAGTAGCAT+GGG | + | Chr7:28953065-28953084 | MsG0780037590.01.T01:intron | 30.0% | |
| AATGAAATATAAGAACAGGG+TGG | + | Chr7:28956573-28956592 | MsG0780037590.01.T01:intron | 30.0% | |
| ACAAATTAGAAACTGAGAAG+AGG | - | Chr7:28954435-28954454 | None:intergenic | 30.0% | |
| ACATCATGACAATTAGTACT+TGG | + | Chr7:28952912-28952931 | MsG0780037590.01.T01:intron | 30.0% | |
| AGAATGTTTGCAATGAAACA+TGG | + | Chr7:28956303-28956322 | MsG0780037590.01.T01:intron | 30.0% | |
| AGACATTGTTAGATATAGAG+TGG | + | Chr7:28953744-28953763 | MsG0780037590.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 28951671 | 28956723 | 28951671 | ID=MsG0780037590.01;Name=MsG0780037590.01 |
| Chr7 | mRNA | 28951671 | 28956723 | 28951671 | ID=MsG0780037590.01.T01;Parent=MsG0780037590.01;Name=MsG0780037590.01.T01;_AED=0.37;_eAED=0.37;_QI=0|0|0|1|1|1|5|0|419 |
| Chr7 | exon | 28951671 | 28952420 | 28951671 | ID=MsG0780037590.01.T01:exon:13446;Parent=MsG0780037590.01.T01 |
| Chr7 | exon | 28952517 | 28952657 | 28952517 | ID=MsG0780037590.01.T01:exon:13447;Parent=MsG0780037590.01.T01 |
| Chr7 | exon | 28952752 | 28952904 | 28952752 | ID=MsG0780037590.01.T01:exon:13448;Parent=MsG0780037590.01.T01 |
| Chr7 | exon | 28953928 | 28954010 | 28953928 | ID=MsG0780037590.01.T01:exon:13449;Parent=MsG0780037590.01.T01 |
| Chr7 | exon | 28956591 | 28956723 | 28956591 | ID=MsG0780037590.01.T01:exon:13450;Parent=MsG0780037590.01.T01 |
| Chr7 | CDS | 28951671 | 28952420 | 28951671 | ID=MsG0780037590.01.T01:cds;Parent=MsG0780037590.01.T01 |
| Chr7 | CDS | 28952517 | 28952657 | 28952517 | ID=MsG0780037590.01.T01:cds;Parent=MsG0780037590.01.T01 |
| Chr7 | CDS | 28952752 | 28952904 | 28952752 | ID=MsG0780037590.01.T01:cds;Parent=MsG0780037590.01.T01 |
| Chr7 | CDS | 28953928 | 28954010 | 28953928 | ID=MsG0780037590.01.T01:cds;Parent=MsG0780037590.01.T01 |
| Chr7 | CDS | 28956591 | 28956723 | 28956591 | ID=MsG0780037590.01.T01:cds;Parent=MsG0780037590.01.T01 |
| Gene Sequence |
>MsG0780037590.01.T01 ATGTCATCCCAGCGTTCAATTCCATCTGTCGATAGAATCAGCAACCTGCCGGACTCCGTTATCTGCCATATCCTCTCCTTCCTCCCTACCAAAACATCTTTAGCCACAAGCATCCTCTCCAAAAGATGGAACCCTTTGTGGCGTTCAGTCCTTACCCTCTACTTCATCGACAACGAGTTTGCAGACTTCAACACTTTTCGACTTTTCGTCTACTCAGTCATGCTCACCCGTGACCCTACCTTGCCCATCCGATTGTTCCACCTCAAATATGGTACCTCCCCCGGGTGTGATCCACATGATATCAATCAATTTGTTCCTGTTGCTGTCAAAAAAGGAATTGAGAATCTCATCTTAGATTTTACTGGCGCTGATTACGATTTTCTAATCGGACTAGGTCCTACACTTTCCAGTATTTTCAATTGCAAGAACCTTGTCGTTCTTAAGTTGAAGAGACTGTTACTCAATGTTGATCCTCAATTCGATCTTCCACTGCTCAAAACTCTCCATTTGGATGACATTTGCCATTTGGGAGATTATGATGAATTTTTTAACAAGATTGTCGAAGGATGTCCCGTTCTAGAGGAATTACAAACAAATGTTCAAGTTCCCTTTCCCTTAAGGGTTCCAAATGATATTGATGGAGGAGAGTTTAAATGCTTTTCCAATTTGGTTAGAGCTAACATTTCTAATTGTAATTGGAATATTCCATTTTCTTGGATTTGTAATGCAAAGTTTCTGCGTCTGGACCTGAGGAATGAGCTAAAAGTTCACACTCTTCATAACTTAACCCACATGGAGCTTATATTTGCATCAAGTTGGCAGAGGAAATGGAAGTGGCTGGTAGAAGTGCTCGAACATTGTCCTAAGCTTCAAAATCTTACTCTTGATCAGCTTTATGGACATGGAAAGGACGAGGATGATTGGAAGAAACCAGAAATTGTTCCAGAATGCATTTCCTCTCAACTTAGAACATGCTCACTTACATCTTATATAGGCAAGAAGTGCGAGCTTCAATTTGCAAAGTATATTATGAAGAATGCAAAAATTCCAACCAAAGATAACCTTCTTGCGTATGGGATATCGTTGGGAGTGACATTCATTAGTGTATATGAGGGTGGTGAAAATCAGGTGGTTAAGAAGAAAGGAGAGACGGAACAGGAGGAACCAAGGTTTACTTTCATGACTAAAAGTGAGGTTGATCAACTAGAAGATGGATGCAAATGGAGAAAATATGGACAGAAAGCTGTCAAGAATATTTGA |
| Protein sequence |
>MsG0780037590.01.T01 MSSQRSIPSVDRISNLPDSVICHILSFLPTKTSLATSILSKRWNPLWRSVLTLYFIDNEFADFNTFRLFVYSVMLTRDPTLPIRLFHLKYGTSPGCDPHDINQFVPVAVKKGIENLILDFTGADYDFLIGLGPTLSSIFNCKNLVVLKLKRLLLNVDPQFDLPLLKTLHLDDICHLGDYDEFFNKIVEGCPVLEELQTNVQVPFPLRVPNDIDGGEFKCFSNLVRANISNCNWNIPFSWICNAKFLRLDLRNELKVHTLHNLTHMELIFASSWQRKWKWLVEVLEHCPKLQNLTLDQLYGHGKDEDDWKKPEIVPECISSQLRTCSLTSYIGKKCELQFAKYIMKNAKIPTKDNLLAYGISLGVTFISVYEGGENQVVKKKGETEQEEPRFTFMTKSEVDQLEDGCKWRKYGQKAVKNI* |
