Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036221.01.T01 | AFK36374.1 | 67.982 | 228 | 3 | 2 | 1 | 158 | 49 | 276 | 2.83E-93 | 281 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036221.01.T01 | Q67YC0 | 36.481 | 233 | 71 | 4 | 1 | 156 | 59 | 291 | 1.21E-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036221.01.T01 | I3S7Y2 | 67.982 | 228 | 3 | 2 | 1 | 158 | 49 | 276 | 1.35e-93 | 281 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010586.01 | MsG0780036221.01 | 0.800406 | 1.463055e-48 | 6.806952e-46 |
| MsG0280011424.01 | MsG0780036221.01 | 0.817218 | 3.695120e-52 | 2.674824e-49 |
| MsG0280007417.01 | MsG0780036221.01 | 0.815674 | 8.190989e-52 | 5.684079e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036221.01.T01 | MTR_2g100340 | 67.982 | 228 | 3 | 2 | 1 | 158 | 49 | 276 | 3.82e-97 | 281 |
| MsG0780036221.01.T01 | MTR_8g015910 | 38.938 | 226 | 64 | 3 | 1 | 156 | 50 | 271 | 5.71e-43 | 144 |
| MsG0780036221.01.T01 | MTR_8g015960 | 37.885 | 227 | 66 | 3 | 1 | 156 | 50 | 272 | 5.13e-41 | 139 |
| MsG0780036221.01.T01 | MTR_2g100350 | 37.778 | 225 | 67 | 3 | 1 | 156 | 49 | 269 | 1.35e-40 | 137 |
| MsG0780036221.01.T01 | MTR_8g015950 | 37.004 | 227 | 68 | 4 | 1 | 156 | 50 | 272 | 4.71e-40 | 136 |
| MsG0780036221.01.T01 | MTR_2g062320 | 38.053 | 226 | 66 | 3 | 1 | 156 | 49 | 270 | 9.12e-40 | 135 |
| MsG0780036221.01.T01 | MTR_8g015940 | 37.383 | 214 | 60 | 3 | 1 | 144 | 50 | 259 | 1.21e-37 | 130 |
| MsG0780036221.01.T01 | MTR_8g101690 | 33.333 | 192 | 56 | 3 | 1 | 121 | 48 | 238 | 4.14e-29 | 107 |
| MsG0780036221.01.T01 | MTR_2g100345 | 73.846 | 65 | 17 | 0 | 93 | 157 | 13 | 77 | 1.13e-28 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036221.01.T01 | AT1G73010 | 36.481 | 233 | 71 | 4 | 1 | 156 | 59 | 291 | 1.23e-35 | 125 |
| MsG0780036221.01.T01 | AT1G17710 | 39.474 | 190 | 44 | 3 | 1 | 119 | 20 | 209 | 7.47e-35 | 122 |
| MsG0780036221.01.T01 | AT1G17710 | 39.474 | 190 | 44 | 3 | 1 | 119 | 52 | 241 | 1.49e-34 | 122 |
| MsG0780036221.01.T01 | AT4G29530 | 32.804 | 189 | 56 | 4 | 1 | 118 | 38 | 226 | 4.89e-23 | 91.3 |
| MsG0780036221.01.T01 | AT4G29530 | 32.804 | 189 | 56 | 4 | 1 | 118 | 49 | 237 | 1.08e-22 | 90.5 |
Find 28 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGGAACTGATAGAGCTTC+AGG | 0.281232 | 7:-4843423 | None:intergenic |
| AAATTGTAGAAGTTCTTAAT+AGG | 0.333392 | 7:+4842141 | MsG0780036221.01.T01:CDS |
| ATCATCAACAAAGCTAATAT+TGG | 0.348472 | 7:+4843318 | MsG0780036221.01.T01:CDS |
| ATTGAGGCTGCTTATTCTCT+AGG | 0.365751 | 7:+4842194 | MsG0780036221.01.T01:CDS |
| CAAAATTCACTTGATGATGA+AGG | 0.378739 | 7:+4843101 | MsG0780036221.01.T01:CDS |
| GTGATAATGCGAGGATGAAT+TGG | 0.382658 | 7:-4842167 | None:intergenic |
| AACAAAGCTAATATTGGTAA+AGG | 0.419483 | 7:+4843324 | MsG0780036221.01.T01:CDS |
| ATCTCAGTTGATTGCAAGAT+GGG | 0.430587 | 7:+4843369 | MsG0780036221.01.T01:CDS |
| AAGAAGAAAATCATCTACTT+AGG | 0.467585 | 7:+4843125 | MsG0780036221.01.T01:CDS |
| ACAAAGCTAATATTGGTAAA+GGG | 0.467952 | 7:+4843325 | MsG0780036221.01.T01:CDS |
| ATTGGTAAAGGGAACATTGT+TGG | 0.470539 | 7:+4843336 | MsG0780036221.01.T01:CDS |
| CATGTACATGGTTGGAGAGA+TGG | 0.479326 | 7:+4843267 | MsG0780036221.01.T01:CDS |
| TAGGAACTGATAGAGCTTCA+GGG | 0.502197 | 7:-4843422 | None:intergenic |
| TAGAGAATAAGCAGCCTCAA+TGG | 0.509736 | 7:-4842192 | None:intergenic |
| TTGAGGCTGCTTATTCTCTA+GGG | 0.512196 | 7:+4842195 | MsG0780036221.01.T01:CDS |
| AAAATCATCTACTTAGGAGA+TGG | 0.512607 | 7:+4843131 | MsG0780036221.01.T01:CDS |
| CATCTCAGTTGATTGCAAGA+TGG | 0.524133 | 7:+4843368 | MsG0780036221.01.T01:CDS |
| CTCACTTTGAGCTTTAGACT+AGG | 0.544849 | 7:-4843170 | None:intergenic |
| GACATAAAGGCACATGTACA+TGG | 0.547678 | 7:+4843255 | MsG0780036221.01.T01:intron |
| TAAAGGCACATGTACATGGT+TGG | 0.564702 | 7:+4843259 | MsG0780036221.01.T01:intron |
| TGACATACAGGGAAAGGTGA+TGG | 0.566260 | 7:+4843070 | MsG0780036221.01.T01:intron |
| ATCTACTTAGGAGATGGAAG+TGG | 0.574030 | 7:+4843137 | MsG0780036221.01.T01:CDS |
| TCGCATTATCACTGCCATTG+AGG | 0.620838 | 7:+4842178 | MsG0780036221.01.T01:CDS |
| ATGAATGAACTTCATGCACA+AGG | 0.639690 | 7:+4842104 | MsG0780036221.01.T01:CDS |
| TTGTGTTCAGGTGTGAGCTG+AGG | 0.644223 | 7:+4842373 | MsG0780036221.01.T01:intron |
| ACATGGTTGGAGAGATGGAG+AGG | 0.648554 | 7:+4843272 | MsG0780036221.01.T01:CDS |
| TCTCAGTTGATTGCAAGATG+GGG | 0.664938 | 7:+4843370 | MsG0780036221.01.T01:CDS |
| TCAATGGCAGTGATAATGCG+AGG | 0.688273 | 7:-4842176 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAATTCAAATGACATAA+AGG | + | Chr7:4843242-4843261 | MsG0780036221.01.T01:intron | 15.0% |
| !! | TAGTATGAGAATAATTTATA+CGG | + | Chr7:4842265-4842284 | MsG0780036221.01.T01:intron | 15.0% |
| !!! | TAAGATGTAATTTTATGTAT+AGG | - | Chr7:4842993-4843012 | None:intergenic | 15.0% |
| !! | AAATTGTAGAAGTTCTTAAT+AGG | + | Chr7:4842141-4842160 | MsG0780036221.01.T01:CDS | 20.0% |
| !! | ATGAATATAATAGCGTTATA+TGG | - | Chr7:4842619-4842638 | None:intergenic | 20.0% |
| !! | TCATCAACAATATATAGAAA+CGG | - | Chr7:4842712-4842731 | None:intergenic | 20.0% |
| ! | AACAAAGCTAATATTGGTAA+AGG | + | Chr7:4843324-4843343 | MsG0780036221.01.T01:CDS | 25.0% |
| ! | AAGAAGAAAATCATCTACTT+AGG | + | Chr7:4843125-4843144 | MsG0780036221.01.T01:CDS | 25.0% |
| ! | AAGATGAAATATTTGTGTTC+AGG | + | Chr7:4842361-4842380 | MsG0780036221.01.T01:intron | 25.0% |
| ! | ACAAAGCTAATATTGGTAAA+GGG | + | Chr7:4843325-4843344 | MsG0780036221.01.T01:CDS | 25.0% |
| ! | ATCATCAACAAAGCTAATAT+TGG | + | Chr7:4843318-4843337 | MsG0780036221.01.T01:CDS | 25.0% |
| ! | ATGGTCATAAATAGATTTAG+AGG | - | Chr7:4842600-4842619 | None:intergenic | 25.0% |
| ! | TAGAAACGGTGAATAAAATA+TGG | - | Chr7:4842698-4842717 | None:intergenic | 25.0% |
| !! | GAGAAGATTTTAGATAATCA+TGG | - | Chr7:4842530-4842549 | None:intergenic | 25.0% |
| !! | GATTATCTAAAATCTTCTCA+TGG | + | Chr7:4842531-4842550 | MsG0780036221.01.T01:intron | 25.0% |
| !!! | AAACAATTTTGAAGCATCAT+GGG | + | Chr7:4842427-4842446 | MsG0780036221.01.T01:CDS | 25.0% |
| AAAATCATCTACTTAGGAGA+TGG | + | Chr7:4843131-4843150 | MsG0780036221.01.T01:CDS | 30.0% | |
| CAAGTTATGTTAGTGAAGAA+GGG | + | Chr7:4842484-4842503 | MsG0780036221.01.T01:intron | 30.0% | |
| CTTCTTCACTAACATAACTT+GGG | - | Chr7:4842485-4842504 | None:intergenic | 30.0% | |
| ! | AGATTTTAGATAATCATGGC+AGG | - | Chr7:4842526-4842545 | None:intergenic | 30.0% |
| ! | CAATCAACTGAGATGATTTT+TGG | - | Chr7:4843363-4843382 | None:intergenic | 30.0% |
| ! | GATTTTAGATAATCATGGCA+GGG | - | Chr7:4842525-4842544 | None:intergenic | 30.0% |
| !! | CAAAATTCACTTGATGATGA+AGG | + | Chr7:4843101-4843120 | MsG0780036221.01.T01:CDS | 30.0% |
| !!! | AACAATTTTGAAGCATCATG+GGG | + | Chr7:4842428-4842447 | MsG0780036221.01.T01:CDS | 30.0% |
| !!! | GAAACAATTTTGAAGCATCA+TGG | + | Chr7:4842426-4842445 | MsG0780036221.01.T01:CDS | 30.0% |
| AGAATTCTGCCTACACAAAA+AGG | + | Chr7:4842883-4842902 | MsG0780036221.01.T01:intron | 35.0% | |
| ATCTCAGTTGATTGCAAGAT+GGG | + | Chr7:4843369-4843388 | MsG0780036221.01.T01:CDS | 35.0% | |
| ATGAATGAACTTCATGCACA+AGG | + | Chr7:4842104-4842123 | MsG0780036221.01.T01:CDS | 35.0% | |
| ATTGGTAAAGGGAACATTGT+TGG | + | Chr7:4843336-4843355 | MsG0780036221.01.T01:CDS | 35.0% | |
| CCAAGTTATGTTAGTGAAGA+AGG | + | Chr7:4842483-4842502 | MsG0780036221.01.T01:intron | 35.0% | |
| CCTTCTTCACTAACATAACT+TGG | - | Chr7:4842486-4842505 | None:intergenic | 35.0% | |
| TCATGCACAAGGAAAAACAA+TGG | + | Chr7:4842115-4842134 | MsG0780036221.01.T01:CDS | 35.0% | |
| ! | ACGATTTTGCCTTTTTGTGT+AGG | - | Chr7:4842895-4842914 | None:intergenic | 35.0% |
| !! | TTGTTGATGACTAATGAACG+TGG | + | Chr7:4842722-4842741 | MsG0780036221.01.T01:intron | 35.0% |
| ATCTACTTAGGAGATGGAAG+TGG | + | Chr7:4843137-4843156 | MsG0780036221.01.T01:CDS | 40.0% | |
| ATTGAGGCTGCTTATTCTCT+AGG | + | Chr7:4842194-4842213 | MsG0780036221.01.T01:CDS | 40.0% | |
| ATTGCCTTGCACATATTTGG+TGG | - | Chr7:4842570-4842589 | None:intergenic | 40.0% | |
| CATCTCAGTTGATTGCAAGA+TGG | + | Chr7:4843368-4843387 | MsG0780036221.01.T01:CDS | 40.0% | |
| GCAATTGCCTTGCACATATT+TGG | - | Chr7:4842573-4842592 | None:intergenic | 40.0% | |
| GTTATGTTAGTGAAGAAGGG+AGG | + | Chr7:4842487-4842506 | MsG0780036221.01.T01:intron | 40.0% | |
| TAGAGAATAAGCAGCCTCAA+TGG | - | Chr7:4842195-4842214 | None:intergenic | 40.0% | |
| TAGGAACTGATAGAGCTTCA+GGG | - | Chr7:4843425-4843444 | None:intergenic | 40.0% | |
| TCTCAGTTGATTGCAAGATG+GGG | + | Chr7:4843370-4843389 | MsG0780036221.01.T01:CDS | 40.0% | |
| TTAGGAACTGATAGAGCTTC+AGG | - | Chr7:4843426-4843445 | None:intergenic | 40.0% | |
| TTGAGGCTGCTTATTCTCTA+GGG | + | Chr7:4842195-4842214 | MsG0780036221.01.T01:CDS | 40.0% | |
| TTGTCCACCAAATATGTGCA+AGG | + | Chr7:4842563-4842582 | MsG0780036221.01.T01:intron | 40.0% | |
| TTTCCCTGTATGTCATTCGA+GGG | - | Chr7:4843065-4843084 | None:intergenic | 40.0% | |
| ! | GACATAAAGGCACATGTACA+TGG | + | Chr7:4843255-4843274 | MsG0780036221.01.T01:intron | 40.0% |
| ! | GTGATAATGCGAGGATGAAT+TGG | - | Chr7:4842170-4842189 | None:intergenic | 40.0% |
| ! | TAAAGGCACATGTACATGGT+TGG | + | Chr7:4843259-4843278 | MsG0780036221.01.T01:intron | 40.0% |
| ! | TGCACATATTTGGTGGACAA+AGG | - | Chr7:4842563-4842582 | None:intergenic | 40.0% |
| ! | TTGACCAGATTTTCCTCCAA+AGG | - | Chr7:4842752-4842771 | None:intergenic | 40.0% |
| !! | CTCACTTTGAGCTTTAGACT+AGG | - | Chr7:4843173-4843192 | None:intergenic | 40.0% |
| CATGTACATGGTTGGAGAGA+TGG | + | Chr7:4843267-4843286 | MsG0780036221.01.T01:CDS | 45.0% | |
| CTCGAATGACATACAGGGAA+AGG | + | Chr7:4843064-4843083 | MsG0780036221.01.T01:intron | 45.0% | |
| CTTTCCCTGTATGTCATTCG+AGG | - | Chr7:4843066-4843085 | None:intergenic | 45.0% | |
| TAATGAACGTGGCATGCCTT+TGG | + | Chr7:4842733-4842752 | MsG0780036221.01.T01:intron | 45.0% | |
| TCGCATTATCACTGCCATTG+AGG | + | Chr7:4842178-4842197 | MsG0780036221.01.T01:CDS | 45.0% | |
| TGACATACAGGGAAAGGTGA+TGG | + | Chr7:4843070-4843089 | MsG0780036221.01.T01:intron | 45.0% | |
| TTCCCTGTATGTCATTCGAG+GGG | - | Chr7:4843064-4843083 | None:intergenic | 45.0% | |
| ! | CATGCCTTTGGAGGAAAATC+TGG | + | Chr7:4842745-4842764 | MsG0780036221.01.T01:intron | 45.0% |
| ! | TCAATGGCAGTGATAATGCG+AGG | - | Chr7:4842179-4842198 | None:intergenic | 45.0% |
| ACATGGTTGGAGAGATGGAG+AGG | + | Chr7:4843272-4843291 | MsG0780036221.01.T01:CDS | 50.0% | |
| TCCCCCTCGAATGACATACA+GGG | + | Chr7:4843059-4843078 | MsG0780036221.01.T01:intron | 50.0% | |
| TCCCTGTATGTCATTCGAGG+GGG | - | Chr7:4843063-4843082 | None:intergenic | 50.0% | |
| TTGTGTTCAGGTGTGAGCTG+AGG | + | Chr7:4842373-4842392 | MsG0780036221.01.T01:intron | 50.0% | |
| CTCCCCCTCGAATGACATAC+AGG | + | Chr7:4843058-4843077 | MsG0780036221.01.T01:intron | 55.0% | |
| TGAACGTGGCATGCCTTTGG+AGG | + | Chr7:4842736-4842755 | MsG0780036221.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 4842101 | 4843449 | 4842101 | ID=MsG0780036221.01;Name=MsG0780036221.01 |
| Chr7 | mRNA | 4842101 | 4843449 | 4842101 | ID=MsG0780036221.01.T01;Parent=MsG0780036221.01;Name=MsG0780036221.01.T01;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|1|1|4|0|158 |
| Chr7 | exon | 4842101 | 4842216 | 4842101 | ID=MsG0780036221.01.T01:exon:2722;Parent=MsG0780036221.01.T01 |
| Chr7 | exon | 4842383 | 4842449 | 4842383 | ID=MsG0780036221.01.T01:exon:2723;Parent=MsG0780036221.01.T01 |
| Chr7 | exon | 4843080 | 4843187 | 4843080 | ID=MsG0780036221.01.T01:exon:2724;Parent=MsG0780036221.01.T01 |
| Chr7 | exon | 4843264 | 4843449 | 4843264 | ID=MsG0780036221.01.T01:exon:2725;Parent=MsG0780036221.01.T01 |
| Chr7 | CDS | 4842101 | 4842216 | 4842101 | ID=MsG0780036221.01.T01:cds;Parent=MsG0780036221.01.T01 |
| Chr7 | CDS | 4842383 | 4842449 | 4842383 | ID=MsG0780036221.01.T01:cds;Parent=MsG0780036221.01.T01 |
| Chr7 | CDS | 4843080 | 4843187 | 4843080 | ID=MsG0780036221.01.T01:cds;Parent=MsG0780036221.01.T01 |
| Chr7 | CDS | 4843264 | 4843449 | 4843264 | ID=MsG0780036221.01.T01:cds;Parent=MsG0780036221.01.T01 |
| Gene Sequence |
| Protein sequence |
