AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011424.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011424.01.T01 MTR_2g104230 98.095 210 3 1 1 210 1 209 3.79e-155 428
MsG0280011424.01.T01 MTR_4g009760 60.181 221 64 10 1 210 1 208 1.63e-81 241
MsG0280011424.01.T01 MTR_4g116200 45.912 159 70 3 1 158 1 144 2.82e-34 122
MsG0280011424.01.T01 MTR_2g450720 38.919 185 85 6 1 165 1 177 9.98e-30 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011424.01.T01 AT1G15760 44.444 225 79 8 3 209 5 201 1.07e-57 181
MsG0280011424.01.T01 AT1G80520 47.489 219 77 8 1 209 1 191 1.08e-50 163
MsG0280011424.01.T01 AT2G12462 29.231 260 121 7 1 209 1 248 5.03e-26 101

Find 41 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAGGCCTAATGGTGGATT+TGG 0.285518 2:+83761139 MsG0280011424.01.T01:CDS
GCTTTGTCTGGTGATCTTGA+TGG 0.316091 2:+83761277 MsG0280011424.01.T01:CDS
GAGAATTTCTAATCTGTGTT+TGG 0.325351 2:-83760975 None:intergenic
TAAGTGGGATACCATGTTTA+AGG 0.337355 2:+83761420 MsG0280011424.01.T01:CDS
GAGAAAATGTTTCTCACAAA+TGG 0.339341 2:+83761220 MsG0280011424.01.T01:CDS
CCATCTCTTGTATATGAGTA+TGG 0.354378 2:+83760866 MsG0280011424.01.T01:CDS
TACTGAAGAATCAGCACTTA+TGG 0.357191 2:+83761108 MsG0280011424.01.T01:CDS
AGTACTTAGCTAACTATGTT+AGG 0.383843 2:+83761074 MsG0280011424.01.T01:CDS
TTCTAATCTGTGTTTGGCTA+TGG 0.404684 2:-83760969 None:intergenic
ATAATCATGATGATGGTTAT+TGG 0.406535 2:+83761377 MsG0280011424.01.T01:CDS
CTAGGCCTAATGGTGGATTT+GGG 0.437057 2:+83761140 MsG0280011424.01.T01:CDS
ACAGTAGCTCCTGCTTTGTC+TGG 0.454059 2:+83761265 MsG0280011424.01.T01:CDS
CACTTATGGTGATGCCAACT+AGG 0.468970 2:+83761122 MsG0280011424.01.T01:CDS
GAAGAGGAACACACAATTTG+TGG 0.477404 2:+83761186 MsG0280011424.01.T01:CDS
CCATGCTTTGTAGAAACTCA+TGG 0.497469 2:-83760940 None:intergenic
TTAACTCAACAGCCATGGAT+TGG 0.500177 2:+83760813 None:intergenic
TTTCCAAAAGGAGCAAAAGA+TGG 0.506781 2:+83761324 MsG0280011424.01.T01:CDS
AATCCACCATTAGGCCTAGT+TGG 0.510240 2:-83761136 None:intergenic
ATGGTGGATTTGGGAGAAGA+TGG 0.518326 2:+83761149 MsG0280011424.01.T01:CDS
CCATGAGTTTCTACAAAGCA+TGG 0.527981 2:+83760940 MsG0280011424.01.T01:CDS
CAAAAGATGGATGGAGATGA+TGG 0.529408 2:+83761337 MsG0280011424.01.T01:CDS
TACAAGAGATGGCTCAAGGT+TGG 0.536104 2:-83760855 None:intergenic
CTGCTGTTGAAGAGATTAAG+TGG 0.538740 2:+83761404 MsG0280011424.01.T01:CDS
CTTCTCCCAAATCCACCATT+AGG 0.539738 2:-83761145 None:intergenic
AATTTGTAGCTGCAATCAAA+AGG 0.542395 2:+83761044 MsG0280011424.01.T01:CDS
GTGATGCCAACTAGGCCTAA+TGG 0.547391 2:+83761130 MsG0280011424.01.T01:CDS
GATGGAGATGATGGTGGTGA+TGG 0.550508 2:+83761346 MsG0280011424.01.T01:CDS
TGTTGTGTATCATTTCCAAA+AGG 0.557274 2:+83761312 MsG0280011424.01.T01:CDS
CAAAAGGAGCAAAAGATGGA+TGG 0.561894 2:+83761328 MsG0280011424.01.T01:CDS
AATAATGATAATCATGATGA+TGG 0.562055 2:+83761370 MsG0280011424.01.T01:CDS
CCATACTCATATACAAGAGA+TGG 0.573633 2:-83760866 None:intergenic
TCAAGATCACCAGACAAAGC+AGG 0.592387 2:-83761274 None:intergenic
CATGAGTTTCTACAAAGCAT+GGG 0.601787 2:+83760941 MsG0280011424.01.T01:CDS
GGAAGAGTGCTATAATGAAG+AGG 0.605141 2:+83761170 MsG0280011424.01.T01:CDS
AAATGTTTCTCACAAATGGA+AGG 0.609404 2:+83761224 MsG0280011424.01.T01:CDS
CATATACAAGAGATGGCTCA+AGG 0.614307 2:-83760859 None:intergenic
TAGCCATGAAAACCAATCCA+TGG 0.623251 2:-83760825 None:intergenic
TGCTGTTGAAGAGATTAAGT+GGG 0.633418 2:+83761405 MsG0280011424.01.T01:CDS
TGGCTTTAGATCCTTAAACA+TGG 0.634954 2:-83761431 None:intergenic
ATGCCAACTAGGCCTAATGG+TGG 0.656564 2:+83761133 MsG0280011424.01.T01:CDS
AAGATGGATGGAGATGATGG+TGG 0.707845 2:+83761340 MsG0280011424.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATGATAATCATGATGA+TGG + Chr2:83761370-83761389 MsG0280011424.01.T01:CDS 20.0%
!!! ATAATCATGATGATGGTTAT+TGG + Chr2:83761377-83761396 MsG0280011424.01.T01:CDS 25.0%
AAATGTTTCTCACAAATGGA+AGG + Chr2:83761224-83761243 MsG0280011424.01.T01:CDS 30.0%
AATTTGTAGCTGCAATCAAA+AGG + Chr2:83761044-83761063 MsG0280011424.01.T01:CDS 30.0%
AGTACTTAGCTAACTATGTT+AGG + Chr2:83761074-83761093 MsG0280011424.01.T01:CDS 30.0%
GAGAAAATGTTTCTCACAAA+TGG + Chr2:83761220-83761239 MsG0280011424.01.T01:CDS 30.0%
GAGAATTTCTAATCTGTGTT+TGG - Chr2:83760978-83760997 None:intergenic 30.0%
TGTTGTGTATCATTTCCAAA+AGG + Chr2:83761312-83761331 MsG0280011424.01.T01:CDS 30.0%
!! TTTTGGAAATGATACACAAC+AGG - Chr2:83761313-83761332 None:intergenic 30.0%
CATGAGTTTCTACAAAGCAT+GGG + Chr2:83760941-83760960 MsG0280011424.01.T01:CDS 35.0%
CCATACTCATATACAAGAGA+TGG - Chr2:83760869-83760888 None:intergenic 35.0%
CCATCTCTTGTATATGAGTA+TGG + Chr2:83760866-83760885 MsG0280011424.01.T01:CDS 35.0%
TAAGTGGGATACCATGTTTA+AGG + Chr2:83761420-83761439 MsG0280011424.01.T01:CDS 35.0%
TACTGAAGAATCAGCACTTA+TGG + Chr2:83761108-83761127 MsG0280011424.01.T01:CDS 35.0%
TGCTGTTGAAGAGATTAAGT+GGG + Chr2:83761405-83761424 MsG0280011424.01.T01:CDS 35.0%
TTCTAATCTGTGTTTGGCTA+TGG - Chr2:83760972-83760991 None:intergenic 35.0%
TTTCCAAAAGGAGCAAAAGA+TGG + Chr2:83761324-83761343 MsG0280011424.01.T01:CDS 35.0%
! ACAAATTTTGCAACTGTGTG+TGG - Chr2:83761031-83761050 None:intergenic 35.0%
!! TGGCTTTAGATCCTTAAACA+TGG - Chr2:83761434-83761453 None:intergenic 35.0%
CAAAAGATGGATGGAGATGA+TGG + Chr2:83761337-83761356 MsG0280011424.01.T01:CDS 40.0%
CAAAAGGAGCAAAAGATGGA+TGG + Chr2:83761328-83761347 MsG0280011424.01.T01:CDS 40.0%
CATATACAAGAGATGGCTCA+AGG - Chr2:83760862-83760881 None:intergenic 40.0%
CCATGAGTTTCTACAAAGCA+TGG + Chr2:83760940-83760959 MsG0280011424.01.T01:CDS 40.0%
CCATGCTTTGTAGAAACTCA+TGG - Chr2:83760943-83760962 None:intergenic 40.0%
CTGCTGTTGAAGAGATTAAG+TGG + Chr2:83761404-83761423 MsG0280011424.01.T01:CDS 40.0%
GAAGAGGAACACACAATTTG+TGG + Chr2:83761186-83761205 MsG0280011424.01.T01:CDS 40.0%
! AAAGCAGGAGCTACTGTTTT+TGG - Chr2:83761262-83761281 None:intergenic 40.0%
! CATCCATCTTTTGCTCCTTT+TGG - Chr2:83761330-83761349 None:intergenic 40.0%
!! GGAAGAGTGCTATAATGAAG+AGG + Chr2:83761170-83761189 MsG0280011424.01.T01:CDS 40.0%
AATCCACCATTAGGCCTAGT+TGG - Chr2:83761139-83761158 None:intergenic 45.0%
CTTCTCCCAAATCCACCATT+AGG - Chr2:83761148-83761167 None:intergenic 45.0%
GCTTTGTCTGGTGATCTTGA+TGG + Chr2:83761277-83761296 MsG0280011424.01.T01:CDS 45.0%
TACAAGAGATGGCTCAAGGT+TGG - Chr2:83760858-83760877 None:intergenic 45.0%
TCAAGATCACCAGACAAAGC+AGG - Chr2:83761277-83761296 None:intergenic 45.0%
! AAGATGGATGGAGATGATGG+TGG + Chr2:83761340-83761359 MsG0280011424.01.T01:CDS 45.0%
! CACTTATGGTGATGCCAACT+AGG + Chr2:83761122-83761141 MsG0280011424.01.T01:CDS 45.0%
! GCTCGTTTTGTGCAAAGACA+AGG - Chr2:83760892-83760911 None:intergenic 45.0%
!! ACTAGGCCTAATGGTGGATT+TGG + Chr2:83761139-83761158 MsG0280011424.01.T01:CDS 45.0%
!! ATGGTGGATTTGGGAGAAGA+TGG + Chr2:83761149-83761168 MsG0280011424.01.T01:CDS 45.0%
!! CTAGGCCTAATGGTGGATTT+GGG + Chr2:83761140-83761159 MsG0280011424.01.T01:CDS 45.0%
!!! AGGAGCTACTGTTTTTGGCA+TGG - Chr2:83761257-83761276 None:intergenic 45.0%
ACAGTAGCTCCTGCTTTGTC+TGG + Chr2:83761265-83761284 MsG0280011424.01.T01:CDS 50.0%
ATGCCAACTAGGCCTAATGG+TGG + Chr2:83761133-83761152 MsG0280011424.01.T01:CDS 50.0%
GTGATGCCAACTAGGCCTAA+TGG + Chr2:83761130-83761149 MsG0280011424.01.T01:CDS 50.0%
!! GATGGAGATGATGGTGGTGA+TGG + Chr2:83761346-83761365 MsG0280011424.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 83760827 83761459 83760827 ID=MsG0280011424.01;Name=MsG0280011424.01
Chr2 mRNA 83760827 83761459 83760827 ID=MsG0280011424.01.T01;Parent=MsG0280011424.01;Name=MsG0280011424.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|210
Chr2 exon 83760827 83761459 83760827 ID=MsG0280011424.01.T01:exon:3132;Parent=MsG0280011424.01.T01
Chr2 CDS 83760827 83761459 83760827 ID=MsG0280011424.01.T01:cds;Parent=MsG0280011424.01.T01
Gene Sequence

>MsG0280011424.01.T01

ATGGATTGGTTTTCATGGCTATCAAAAACCAACCTTGAGCCATCTCTTGTATATGAGTATGGCCTTGTCTTTGCACAAAACGAGCTTGAAGAAGAAGACATGTTTTACTTCAACCATGAGTTTCTACAAAGCATGGGAATCTCCATAGCCAAACACAGATTAGAAATTCTCAAGCTTGCTAAAAAAGACAAGAGAAAAAGTCCACACACAGTTGCAAAATTTGTAGCTGCAATCAAAAGGACAAAGAAGTACTTAGCTAACTATGTTAGGACTTTGACTCATACTGAAGAATCAGCACTTATGGTGATGCCAACTAGGCCTAATGGTGGATTTGGGAGAAGATGGAAGAGTGCTATAATGAAGAGGAACACACAATTTGTGGTGTCTAAGCAAGAGAAAATGTTTCTCACAAATGGAAGGTCTTCTTCCATGCCAAAAACAGTAGCTCCTGCTTTGTCTGGTGATCTTGATGGTTTTTCTAGTCCTGTTGTGTATCATTTCCAAAAGGAGCAAAAGATGGATGGAGATGATGGTGGTGATGGTAATAATGATAATCATGATGATGGTTATTGGTCTGCTGCTGTTGAAGAGATTAAGTGGGATACCATGTTTAAGGATCTAAAGCCAAACTAA

Protein sequence

>MsG0280011424.01.T01

MDWFSWLSKTNLEPSLVYEYGLVFAQNELEEEDMFYFNHEFLQSMGISIAKHRLEILKLAKKDKRKSPHTVAKFVAAIKRTKKYLANYVRTLTHTEESALMVMPTRPNGGFGRRWKSAIMKRNTQFVVSKQEKMFLTNGRSSSMPKTVAPALSGDLDGFSSPVVYHFQKEQKMDGDDGGDGNNDNHDDGYWSAAVEEIKWDTMFKDLKPN*