Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036374.01.T01 | MCH84272.1 | 80.952 | 63 | 12 | 0 | 2 | 64 | 164 | 226 | 7.70E-29 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036374.01.T01 | P08688 | 79.365 | 63 | 13 | 0 | 2 | 64 | 165 | 227 | 6.50E-31 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036374.01.T01 | A0A392M9W4 | 80.952 | 63 | 12 | 0 | 2 | 64 | 164 | 226 | 3.68e-29 | 112 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000582.01 | MsG0780036374.01 | 0.804722 | 1.884400e-49 | 9.788259e-47 |
| MsG0180002024.01 | MsG0780036374.01 | 0.801839 | 7.449447e-49 | 3.593970e-46 |
| MsG0180004106.01 | MsG0780036374.01 | 0.813453 | 2.539779e-51 | 1.659386e-48 |
| MsG0380014794.01 | MsG0780036374.01 | 0.819355 | 1.213407e-52 | 9.315006e-50 |
| MsG0480022918.01 | MsG0780036374.01 | 0.810341 | 1.209872e-50 | 7.274025e-48 |
| MsG0580029414.01 | MsG0780036374.01 | 0.803843 | 2.872902e-49 | 1.458862e-46 |
| MsG0280009507.01 | MsG0780036374.01 | 0.824827 | 6.537205e-54 | 5.855963e-51 |
| MsG0780036374.01 | MsG0780041262.01 | 0.815003 | 1.155098e-51 | 7.869899e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036374.01.T01 | MTR_7g012000 | 92.593 | 54 | 4 | 0 | 1 | 54 | 135 | 188 | 1.13e-30 | 106 |
| MsG0780036374.01.T01 | MTR_0057s0100 | 70.000 | 60 | 18 | 0 | 2 | 61 | 164 | 223 | 3.07e-24 | 90.5 |
| MsG0780036374.01.T01 | MTR_0057s0170 | 68.333 | 60 | 19 | 0 | 2 | 61 | 164 | 223 | 1.16e-23 | 89.0 |
| MsG0780036374.01.T01 | MTR_0695s0030 | 59.016 | 61 | 24 | 1 | 5 | 64 | 224 | 284 | 6.00e-19 | 77.8 |
| MsG0780036374.01.T01 | MTR_8g038070 | 60.656 | 61 | 23 | 1 | 5 | 64 | 378 | 438 | 1.01e-18 | 78.2 |
| MsG0780036374.01.T01 | MTR_8g038100 | 59.016 | 61 | 24 | 1 | 5 | 64 | 440 | 500 | 2.70e-18 | 77.0 |
| MsG0780036374.01.T01 | MTR_7g103990 | 59.322 | 59 | 23 | 1 | 3 | 60 | 165 | 223 | 3.83e-18 | 74.7 |
| MsG0780036374.01.T01 | MTR_1g066530 | 58.065 | 62 | 23 | 2 | 5 | 65 | 162 | 221 | 1.04e-16 | 70.9 |
| MsG0780036374.01.T01 | MTR_6g040290 | 50.847 | 59 | 28 | 1 | 3 | 60 | 167 | 225 | 3.45e-12 | 58.9 |
| MsG0780036374.01.T01 | MTR_6g073540 | 50.000 | 62 | 29 | 2 | 5 | 64 | 168 | 229 | 8.49e-12 | 57.8 |
| MsG0780036374.01.T01 | MTR_0807s0020 | 43.548 | 62 | 30 | 1 | 3 | 64 | 162 | 218 | 1.12e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAACATATGACTACATTA+TGG | 0.354973 | 7:+7220330 | MsG0780036374.01.T01:CDS |
| CGCGGCACGATCTTTGAGAA+TGG | 0.399505 | 7:+7220220 | MsG0780036374.01.T01:CDS |
| GACTCATGGCCTTCTCTTCG+CGG | 0.470021 | 7:+7220373 | MsG0780036374.01.T01:CDS |
| CGCAGGCTGCCGCGAAGAGA+AGG | 0.471500 | 7:-7220382 | None:intergenic |
| GCAGTTAAGTTTATGACCGC+AGG | 0.490532 | 7:-7220399 | None:intergenic |
| ATGTAGTCATATGTTGTGCC+TGG | 0.496580 | 7:-7220325 | None:intergenic |
| CTTCTCTTCGCGGCAGCCTG+CGG | 0.527059 | 7:+7220383 | MsG0780036374.01.T01:CDS |
| TGAGGCGCACTCTTGACTCA+TGG | 0.547474 | 7:+7220359 | MsG0780036374.01.T01:CDS |
| TGTAGTCATATGTTGTGCCT+GGG | 0.584021 | 7:-7220324 | None:intergenic |
| ACATATGACTACATTATGGA+TGG | 0.586278 | 7:+7220334 | MsG0780036374.01.T01:CDS |
| GACGGGTTTATGTGTTTCCG+CGG | 0.617152 | 7:+7220202 | None:intergenic |
| GCACGTGTCACTATTACCCC+AGG | 0.617209 | 7:+7220307 | MsG0780036374.01.T01:CDS |
| GTAGTCATATGTTGTGCCTG+GGG | 0.715856 | 7:-7220323 | None:intergenic |
| CATTCTCAAAGATCGTGCCG+CGG | 0.721328 | 7:-7220219 | None:intergenic |
| ACTACATTATGGATGGTGTG+AGG | 0.755157 | 7:+7220341 | MsG0780036374.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGAAGCTTACTTTTTCAA+AGG | + | Chr7:7220274-7220293 | MsG0780036374.01.T01:CDS | 25.0% |
| ACATATGACTACATTATGGA+TGG | + | Chr7:7220334-7220353 | MsG0780036374.01.T01:CDS | 30.0% | |
| CACAACATATGACTACATTA+TGG | + | Chr7:7220330-7220349 | MsG0780036374.01.T01:CDS | 30.0% | |
| ACTACATTATGGATGGTGTG+AGG | + | Chr7:7220341-7220360 | MsG0780036374.01.T01:CDS | 40.0% | |
| ATGTAGTCATATGTTGTGCC+TGG | - | Chr7:7220328-7220347 | None:intergenic | 40.0% | |
| TGTAGTCATATGTTGTGCCT+GGG | - | Chr7:7220327-7220346 | None:intergenic | 40.0% | |
| GTAGTCATATGTTGTGCCTG+GGG | - | Chr7:7220326-7220345 | None:intergenic | 45.0% | |
| CATTCTCAAAGATCGTGCCG+CGG | - | Chr7:7220222-7220241 | None:intergenic | 50.0% | |
| GACTCATGGCCTTCTCTTCG+CGG | + | Chr7:7220373-7220392 | MsG0780036374.01.T01:CDS | 55.0% | |
| TGAGGCGCACTCTTGACTCA+TGG | + | Chr7:7220359-7220378 | MsG0780036374.01.T01:CDS | 55.0% | |
| ! | CGCGGCACGATCTTTGAGAA+TGG | + | Chr7:7220220-7220239 | MsG0780036374.01.T01:CDS | 55.0% |
| ! | GCACGTGTCACTATTACCCC+AGG | + | Chr7:7220307-7220326 | MsG0780036374.01.T01:CDS | 55.0% |
| CTTCTCTTCGCGGCAGCCTG+CGG | + | Chr7:7220383-7220402 | MsG0780036374.01.T01:CDS | 65.0% | |
| CGCAGGCTGCCGCGAAGAGA+AGG | - | Chr7:7220385-7220404 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 7220211 | 7220411 | 7220211 | ID=MsG0780036374.01;Name=MsG0780036374.01 |
| Chr7 | mRNA | 7220211 | 7220411 | 7220211 | ID=MsG0780036374.01.T01;Parent=MsG0780036374.01;Name=MsG0780036374.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|66 |
| Chr7 | exon | 7220211 | 7220411 | 7220211 | ID=MsG0780036374.01.T01:exon:3845;Parent=MsG0780036374.01.T01 |
| Chr7 | CDS | 7220211 | 7220411 | 7220211 | ID=MsG0780036374.01.T01:cds;Parent=MsG0780036374.01.T01 |
| Gene Sequence |
| Protein sequence |
