Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036475.01.T01 | XP_039684049.1 | 100 | 92 | 0 | 0 | 3 | 94 | 2 | 93 | 1.77E-58 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036475.01.T01 | B5FZA8 | 37.179 | 78 | 49 | 0 | 5 | 82 | 2 | 79 | 2.86E-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036475.01.T01 | G7L496 | 100.000 | 92 | 0 | 0 | 3 | 94 | 2 | 93 | 8.44e-59 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680031130.01 | MsG0780036475.01 | 0.818694 | 1.714978e-52 | 1.292779e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036475.01.T01 | MTR_7g012870 | 100.000 | 92 | 0 | 0 | 3 | 94 | 2 | 93 | 2.14e-62 | 184 |
| MsG0780036475.01.T01 | MTR_0287s0050 | 100.000 | 92 | 0 | 0 | 3 | 94 | 2 | 93 | 2.14e-62 | 184 |
| MsG0780036475.01.T01 | MTR_7g083770 | 75.641 | 78 | 19 | 0 | 17 | 94 | 55 | 132 | 6.42e-39 | 126 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036475.01.T01 | AT5G61220 | 47.561 | 82 | 43 | 0 | 8 | 89 | 3 | 84 | 1.29e-23 | 86.3 |
| MsG0780036475.01.T01 | AT5G61220 | 47.561 | 82 | 43 | 0 | 8 | 89 | 3 | 84 | 1.29e-23 | 86.3 |
| MsG0780036475.01.T01 | AT5G61220 | 47.561 | 82 | 43 | 0 | 8 | 89 | 3 | 84 | 1.29e-23 | 86.3 |
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGATTAAAAGGGTTTATT+AGG | 0.211621 | 7:-8669229 | None:intergenic |
| GCGAAGAAGGGTCAGGGTTT+GGG | 0.299810 | 7:-8668990 | None:intergenic |
| AGCGAAGAAGGGTCAGGGTT+TGG | 0.302465 | 7:-8668991 | None:intergenic |
| ATTTCCGAAGCTTTCTCGTT+TGG | 0.370472 | 7:+8669124 | MsG0780036475.01.T01:CDS |
| TCCTTCTCTTCCAAGTGTTA+TGG | 0.372181 | 7:+8669201 | MsG0780036475.01.T01:CDS |
| TATTCGCGTATGTTGTAATC+TGG | 0.381263 | 7:-8669040 | None:intergenic |
| GTATGTTGTAATCTGGGAAT+TGG | 0.427288 | 7:-8669033 | None:intergenic |
| AAAACGAACCTTGATTAAAA+GGG | 0.427832 | 7:-8669238 | None:intergenic |
| CGCGGATGAAGGAGCGAAGA+AGG | 0.438435 | 7:-8669003 | None:intergenic |
| TGAAGGAGCGAAGAAGGGTC+AGG | 0.450638 | 7:-8668997 | None:intergenic |
| TTGGCGAGAAACGCGGATGA+AGG | 0.517583 | 7:-8669014 | None:intergenic |
| TCCATAACACTTGGAAGAGA+AGG | 0.524453 | 7:-8669202 | None:intergenic |
| ATTCGCGTATGTTGTAATCT+GGG | 0.539311 | 7:-8669039 | None:intergenic |
| GTCGACGACTATGGCGGCGG+CGG | 0.544766 | 7:+8668952 | None:intergenic |
| TTAGGAAGATCCATAACACT+TGG | 0.559900 | 7:-8669211 | None:intergenic |
| CCCGTCGACGACTATGGCGG+CGG | 0.566090 | 7:+8668949 | None:intergenic |
| CTGGGAATTGGCGAGAAACG+CGG | 0.581220 | 7:-8669021 | None:intergenic |
| GCGGATGAAGGAGCGAAGAA+GGG | 0.615879 | 7:-8669002 | None:intergenic |
| GAAGGAGCGAAGAAGGGTCA+GGG | 0.629779 | 7:-8668996 | None:intergenic |
| AGCATAAATAGAGTAAACAA+CGG | 0.670929 | 7:-8669179 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGCATAAATAGAGTAAACAA+CGG | - | Chr7:8669182-8669201 | None:intergenic | 25.0% |
| ATTCGCGTATGTTGTAATCT+GGG | - | Chr7:8669042-8669061 | None:intergenic | 35.0% | |
| GTATGTTGTAATCTGGGAAT+TGG | - | Chr7:8669036-8669055 | None:intergenic | 35.0% | |
| TATTCGCGTATGTTGTAATC+TGG | - | Chr7:8669043-8669062 | None:intergenic | 35.0% | |
| TTAGGAAGATCCATAACACT+TGG | - | Chr7:8669214-8669233 | None:intergenic | 35.0% | |
| ! | TTTTCCAAACGAGAAAGCTT+CGG | - | Chr7:8669131-8669150 | None:intergenic | 35.0% |
| ATTTCCGAAGCTTTCTCGTT+TGG | + | Chr7:8669124-8669143 | MsG0780036475.01.T01:CDS | 40.0% | |
| TCCATAACACTTGGAAGAGA+AGG | - | Chr7:8669205-8669224 | None:intergenic | 40.0% | |
| TCCTTCTCTTCCAAGTGTTA+TGG | + | Chr7:8669201-8669220 | MsG0780036475.01.T01:CDS | 40.0% | |
| !! | GTCAGGGTTTGGGATTTTGA+AGG | - | Chr7:8668983-8669002 | None:intergenic | 45.0% |
| !! | GTTTTGACGAAACGCATCGA+TGG | - | Chr7:8669080-8669099 | None:intergenic | 45.0% |
| AGCGAAGAAGGGTCAGGGTT+TGG | - | Chr7:8668994-8669013 | None:intergenic | 55.0% | |
| CTGGGAATTGGCGAGAAACG+CGG | - | Chr7:8669024-8669043 | None:intergenic | 55.0% | |
| GAAGGAGCGAAGAAGGGTCA+GGG | - | Chr7:8668999-8669018 | None:intergenic | 55.0% | |
| GCGAAGAAGGGTCAGGGTTT+GGG | - | Chr7:8668993-8669012 | None:intergenic | 55.0% | |
| GCGGATGAAGGAGCGAAGAA+GGG | - | Chr7:8669005-8669024 | None:intergenic | 55.0% | |
| TGAAGGAGCGAAGAAGGGTC+AGG | - | Chr7:8669000-8669019 | None:intergenic | 55.0% | |
| TTGGCGAGAAACGCGGATGA+AGG | - | Chr7:8669017-8669036 | None:intergenic | 55.0% | |
| CGCGGATGAAGGAGCGAAGA+AGG | - | Chr7:8669006-8669025 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 8668962 | 8669246 | 8668962 | ID=MsG0780036475.01;Name=MsG0780036475.01 |
| Chr7 | mRNA | 8668962 | 8669246 | 8668962 | ID=MsG0780036475.01.T01;Parent=MsG0780036475.01;Name=MsG0780036475.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|94 |
| Chr7 | exon | 8668962 | 8669246 | 8668962 | ID=MsG0780036475.01.T01:exon:1749;Parent=MsG0780036475.01.T01 |
| Chr7 | CDS | 8668962 | 8669246 | 8668962 | ID=MsG0780036475.01.T01:cds;Parent=MsG0780036475.01.T01 |
| Gene Sequence |
| Protein sequence |
