AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036478.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGAAGGGTTTGTGATTT+GGG 0.232203 7:+8712377 None:intergenic
TGGGTTGGAGGAGGTGGTGG+TGG 0.257478 7:+8712551 None:intergenic
GTTGGAGGAGGTGGTGGTGG+TGG 0.273057 7:+8712554 None:intergenic
GGTGGTGGTGGTGGAGTTGG+TGG 0.277657 7:+8712563 None:intergenic
GATGGGGTGGTTGTGGTGTG+GGG 0.278446 7:+8712495 None:intergenic
AAGATGGGGTGGTTGTGGTG+TGG 0.309946 7:+8712493 None:intergenic
TTGATGAAGGGTTTGTGATT+TGG 0.314957 7:+8712376 None:intergenic
GGAGGTGGTGGTGGTGGAGT+TGG 0.319875 7:+8712560 None:intergenic
AGATGGGGTGGTTGTGGTGT+GGG 0.335949 7:+8712494 None:intergenic
GGAGTTGGTGGTGGCTGTGA+TGG 0.346772 7:+8712575 None:intergenic
CAATGGGTTGGAGGAGGTGG+TGG 0.351890 7:+8712548 None:intergenic
GGGGTGTAGCATGGGTAAGA+GGG 0.352158 7:+8712515 None:intergenic
ATGGGGTGGTTGTGGTGTGG+GGG 0.370454 7:+8712496 None:intergenic
GGTGGTGGTGGAGTTGGTGG+TGG 0.376758 7:+8712566 None:intergenic
GGGTGTAGCATGGGTAAGAG+GGG 0.380958 7:+8712516 None:intergenic
GTGCAGTAGCAATGGGTTGG+AGG 0.393924 7:+8712539 None:intergenic
GTCGTGTTGTGGAGAAAGAT+GGG 0.404514 7:+8712478 None:intergenic
GGAGAAAGATGGGGTGGTTG+TGG 0.411329 7:+8712488 None:intergenic
TAGCAATGGGTTGGAGGAGG+TGG 0.415086 7:+8712545 None:intergenic
AGAAAGGAGGATGGAGGAAA+TGG 0.423348 7:+8712442 None:intergenic
GTTGATTGAGGGAAGATTCA+AGG 0.442554 7:+8712306 None:intergenic
GGTCGTGTTGTGGAGAAAGA+TGG 0.442953 7:+8712477 None:intergenic
GGCTGTGATGGTGGTTGTAG+TGG 0.443051 7:+8712587 None:intergenic
GGAGAGAAGAGAGTGTAGAT+TGG 0.446189 7:+8712345 None:intergenic
GGTGGTTGAGTTGTGGAGAA+AGG 0.452850 7:+8712426 None:intergenic
GTTGGTGGTGGCTGTGATGG+TGG 0.456510 7:+8712578 None:intergenic
GGTGTAGCATGGGTAAGAGG+GGG 0.467114 7:+8712517 None:intergenic
GAAGGGTTTGTGATTTGGGG+TGG 0.485747 7:+8712381 None:intergenic
GATGAGTTTGATGTTGATTG+AGG 0.494626 7:+8712294 None:intergenic
GAGTTGTGGAGAAAGGAGGA+TGG 0.519893 7:+8712433 None:intergenic
CAGTAGCAATGGGTTGGAGG+AGG 0.524583 7:+8712542 None:intergenic
CAAGGGTTTCAATGCGGTGA+AGG 0.527850 7:+8712324 None:intergenic
TTGATTGAGGGAAGATTCAA+GGG 0.529353 7:+8712307 None:intergenic
AACACTCACACTCAGCTGAA+TGG 0.543056 7:-8712620 MsG0780036478.01.T01:exon
ATGAGTTTGATGTTGATTGA+GGG 0.550394 7:+8712295 None:intergenic
GATGAAGGGTTTGTGATTTG+GGG 0.561923 7:+8712378 None:intergenic
TTGTGGAGAAAGGAGGATGG+AGG 0.563095 7:+8712436 None:intergenic
TGTGATGGTGGTTGTAGTGG+TGG 0.564747 7:+8712590 None:intergenic
TGTACGAGGTGGTCGTGTTG+TGG 0.568594 7:+8712467 None:intergenic
GTGTGAGTGTTACTCCCTCA+AGG 0.570318 7:+8712632 None:intergenic
TGTACGAGGTGGTTGAGTTG+TGG 0.578566 7:+8712419 None:intergenic
GTGATTTGGGGTGGTGTACG+AGG 0.588657 7:+8712390 None:intergenic
GGGGTGCAGTAGCAATGGGT+TGG 0.592304 7:+8712536 None:intergenic
GGTTGAGTTGTGGAGAAAGG+AGG 0.595377 7:+8712429 None:intergenic
TTACACGTGCTGCGGAGTGT+CGG 0.611191 7:+8712258 None:intergenic
GTGGTTGTAGTGGTGGTGAG+AGG 0.617629 7:+8712597 None:intergenic
TGTGAGTGTTACTCCCTCAA+GGG 0.634914 7:+8712633 None:intergenic
TGTTGTGGAGAAAGATGGGG+TGG 0.652811 7:+8712482 None:intergenic
TCGTGTTGTGGAGAAAGATG+GGG 0.652928 7:+8712479 None:intergenic
GGTTTGGATTACACGTGCTG+CGG 0.656835 7:+8712250 None:intergenic
AGGAAATGGTTTGTACGAGG+TGG 0.672405 7:+8712456 None:intergenic
TGGAGGAAATGGTTTGTACG+AGG 0.681151 7:+8712453 None:intergenic
GTACGAGGTTGTCGTGTACG+AGG 0.710331 7:+8712405 None:intergenic
AAGATTCAAGGGTTTCAATG+CGG 0.722631 7:+8712318 None:intergenic
CGAGGTTGTCGTGTACGAGG+TGG 0.759932 7:+8712408 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAAAACAATAGTCCCTTGA+GGG - Chr7:8712330-8712349 MsG0780036478.01.T01:CDS 30.0%
! ATGAGTTTGATGTTGATTGA+GGG + Chr7:8712684-8712703 None:intergenic 30.0%
!! ATTGGTGATTTTGTTGATGA+AGG + Chr7:8712616-8712635 None:intergenic 30.0%
!! TTGGTGATTTTGTTGATGAA+GGG + Chr7:8712615-8712634 None:intergenic 30.0%
AAGATTCAAGGGTTTCAATG+CGG + Chr7:8712661-8712680 None:intergenic 35.0%
CAAAAAACAATAGTCCCTTG+AGG - Chr7:8712329-8712348 MsG0780036478.01.T01:CDS 35.0%
TGATGAAGGGTTTGTGATTT+GGG + Chr7:8712602-8712621 None:intergenic 35.0%
TTGATGAAGGGTTTGTGATT+TGG + Chr7:8712603-8712622 None:intergenic 35.0%
TTGATTGAGGGAAGATTCAA+GGG + Chr7:8712672-8712691 None:intergenic 35.0%
! GATGAGTTTGATGTTGATTG+AGG + Chr7:8712685-8712704 None:intergenic 35.0%
GATGAAGGGTTTGTGATTTG+GGG + Chr7:8712601-8712620 None:intergenic 40.0%
GTTGATTGAGGGAAGATTCA+AGG + Chr7:8712673-8712692 None:intergenic 40.0%
AACACTCACACTCAGCTGAA+TGG - Chr7:8712356-8712375 MsG0780036478.01.T01:CDS 45.0%
AGAAAGGAGGATGGAGGAAA+TGG + Chr7:8712537-8712556 None:intergenic 45.0%
GTCGTGTTGTGGAGAAAGAT+GGG + Chr7:8712501-8712520 None:intergenic 45.0%
TCGTGTTGTGGAGAAAGATG+GGG + Chr7:8712500-8712519 None:intergenic 45.0%
! AGGAAATGGTTTGTACGAGG+TGG + Chr7:8712523-8712542 None:intergenic 45.0%
! GGAGAGAAGAGAGTGTAGAT+TGG + Chr7:8712634-8712653 None:intergenic 45.0%
! TGGAGGAAATGGTTTGTACG+AGG + Chr7:8712526-8712545 None:intergenic 45.0%
! TGTGAGTGTTACTCCCTCAA+GGG + Chr7:8712346-8712365 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAGAG+AGG + Chr7:8712264-8712283 None:intergenic 50.0%
CAAGGGTTTCAATGCGGTGA+AGG + Chr7:8712655-8712674 None:intergenic 50.0%
GAGTTGTGGAGAAAGGAGGA+TGG + Chr7:8712546-8712565 None:intergenic 50.0%
GGTCGTGTTGTGGAGAAAGA+TGG + Chr7:8712502-8712521 None:intergenic 50.0%
TGTTGTGGAGAAAGATGGGG+TGG + Chr7:8712497-8712516 None:intergenic 50.0%
TTGTGGAGAAAGGAGGATGG+AGG + Chr7:8712543-8712562 None:intergenic 50.0%
! GAAGGGTTTGTGATTTGGGG+TGG + Chr7:8712598-8712617 None:intergenic 50.0%
! GGTGGTTGAGTTGTGGAGAA+AGG + Chr7:8712553-8712572 None:intergenic 50.0%
! GGTTGAGTTGTGGAGAAAGG+AGG + Chr7:8712550-8712569 None:intergenic 50.0%
! GTGTGAGTGTTACTCCCTCA+AGG + Chr7:8712347-8712366 None:intergenic 50.0%
! TGTACGAGGTGGTTGAGTTG+TGG + Chr7:8712560-8712579 None:intergenic 50.0%
!! TGTGATGGTGGTTGTAGTGG+TGG + Chr7:8712389-8712408 None:intergenic 50.0%
AAGATGGGGTGGTTGTGGTG+TGG + Chr7:8712486-8712505 None:intergenic 55.0%
AGATGGGGTGGTTGTGGTGT+GGG + Chr7:8712485-8712504 None:intergenic 55.0%
GGAGAAAGATGGGGTGGTTG+TGG + Chr7:8712491-8712510 None:intergenic 55.0%
GGGGTGTAGCATGGGTAAGA+GGG + Chr7:8712464-8712483 None:intergenic 55.0%
GGGTGTAGCATGGGTAAGAG+GGG + Chr7:8712463-8712482 None:intergenic 55.0%
GGTGTAGCATGGGTAAGAGG+GGG + Chr7:8712462-8712481 None:intergenic 55.0%
GTACGAGGTTGTCGTGTACG+AGG + Chr7:8712574-8712593 None:intergenic 55.0%
TGTACGAGGTGGTCGTGTTG+TGG + Chr7:8712512-8712531 None:intergenic 55.0%
TTACACGTGCTGCGGAGTGT+CGG + Chr7:8712721-8712740 None:intergenic 55.0%
! AAGAGGGGGTGCAGTAGCAA+TGG + Chr7:8712448-8712467 None:intergenic 55.0%
! AGAGGGGGTGCAGTAGCAAT+GGG + Chr7:8712447-8712466 None:intergenic 55.0%
! GTGATTTGGGGTGGTGTACG+AGG + Chr7:8712589-8712608 None:intergenic 55.0%
! GTGGTTGTAGTGGTGGTGAG+AGG + Chr7:8712382-8712401 None:intergenic 55.0%
!! CAGTAGCAATGGGTTGGAGG+AGG + Chr7:8712437-8712456 None:intergenic 55.0%
!! GGCTGTGATGGTGGTTGTAG+TGG + Chr7:8712392-8712411 None:intergenic 55.0%
!! GTGCAGTAGCAATGGGTTGG+AGG + Chr7:8712440-8712459 None:intergenic 55.0%
!! TAGCAATGGGTTGGAGGAGG+TGG + Chr7:8712434-8712453 None:intergenic 55.0%
ATGGGGTGGTTGTGGTGTGG+GGG + Chr7:8712483-8712502 None:intergenic 60.0%
CGAGGTTGTCGTGTACGAGG+TGG + Chr7:8712571-8712590 None:intergenic 60.0%
GATGGGGTGGTTGTGGTGTG+GGG + Chr7:8712484-8712503 None:intergenic 60.0%
GGGGTGCAGTAGCAATGGGT+TGG + Chr7:8712443-8712462 None:intergenic 60.0%
! GGGGGTGTAGCATGGGTAAG+AGG + Chr7:8712465-8712484 None:intergenic 60.0%
! GTGGTGTGGGGGTGTAGCAT+GGG + Chr7:8712472-8712491 None:intergenic 60.0%
! TGTGGTGTGGGGGTGTAGCA+TGG + Chr7:8712473-8712492 None:intergenic 60.0%
!! CAATGGGTTGGAGGAGGTGG+TGG + Chr7:8712431-8712450 None:intergenic 60.0%
!! GGAGTTGGTGGTGGCTGTGA+TGG + Chr7:8712404-8712423 None:intergenic 60.0%
!! GTTGGTGGTGGCTGTGATGG+TGG + Chr7:8712401-8712420 None:intergenic 60.0%
! TGGGTTGGAGGAGGTGGTGG+TGG + Chr7:8712428-8712447 None:intergenic 65.0%
!! GGAGGTGGTGGTGGTGGAGT+TGG + Chr7:8712419-8712438 None:intergenic 65.0%
!! GGTGGTGGTGGAGTTGGTGG+TGG + Chr7:8712413-8712432 None:intergenic 65.0%
!! GGTGGTGGTGGTGGAGTTGG+TGG + Chr7:8712416-8712435 None:intergenic 65.0%
!! GTTGGAGGAGGTGGTGGTGG+TGG + Chr7:8712425-8712444 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 8712258 8712740 8712258 ID=MsG0780036478.01;Name=MsG0780036478.01
Chr7 mRNA 8712258 8712740 8712258 ID=MsG0780036478.01.T01;Parent=MsG0780036478.01;Name=MsG0780036478.01.T01;_AED=0.34;_eAED=0.34;_QI=117|1|1|1|0|0.5|2|0|121
Chr7 exon 8712258 8712740 8712258 ID=MsG0780036478.01.T01:exon:1406;Parent=MsG0780036478.01.T01
Chr7 five_prime_UTR 8712624 8712740 8712624 ID=MsG0780036478.01.T01:five_prime_utr;Parent=MsG0780036478.01.T01
Chr7 CDS 8712258 8712623 8712258 ID=MsG0780036478.01.T01:cds;Parent=MsG0780036478.01.T01
Gene Sequence

>MsG0780036478.01.T01

ATGGCCTCTCACCACCACTACAACCACCATCACAGCCACCACCAACTCCACCACCACCACCTCCTCCAACCCATTGCTACTGCACCCCCTCTTACCCATGCTACACCCCCACACCACAACCACCCCATCTTTCTCCACAACACGACCACCTCGTACAAACCATTTCCTCCATCCTCCTTTCTCCACAACTCAACCACCTCGTACACGACAACCTCGTACACCACCCCAAATCACAAACCCTTCATCAACAAAATCACCAATCTACACTCTCTTCTCTCCTTCACCGCATTGAAACCCTTGAATCTTCCCTCAATCAACATCAAACTCATCAATCTCAATCTCTCCGACACTCCGCAGCACGTGTAA
Protein sequence

>MsG0780036478.01.T01

MASHHHYNHHHSHHQLHHHHLLQPIATAPPLTHATPPHHNHPIFLHNTTTSYKPFPPSSFLHNSTTSYTTTSYTTPNHKPFINKITNLHSLLSFTALKPLNLPSINIKLINLNLSDTPQHV*