AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040459.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040459.01.T01 MTR_7g089400 87.200 125 15 1 1 125 1 124 1.56e-74 223
MsG0780040459.01.T01 MTR_1g093750 49.215 191 90 3 59 243 141 330 1.26e-47 161
MsG0780040459.01.T01 MTR_3g070970 35.317 252 137 6 14 243 7 254 4.18e-42 144
MsG0780040459.01.T01 MTR_2g091190 46.369 179 89 3 71 243 54 231 4.11e-34 123
MsG0780040459.01.T01 MTR_2g015890 38.636 176 99 3 71 241 62 233 5.92e-31 115
MsG0780040459.01.T01 MTR_4g079760 42.021 188 92 5 68 243 60 242 3.16e-28 108
MsG0780040459.01.T01 MTR_2g015890 41.880 117 64 1 71 187 62 174 9.89e-25 98.2
MsG0780040459.01.T01 MTR_5g066080 39.080 174 78 3 59 206 19 190 1.25e-23 96.3
MsG0780040459.01.T01 MTR_5g032375 32.093 215 118 6 32 233 7 206 5.36e-22 90.9
MsG0780040459.01.T01 MTR_4g087920 37.805 164 97 4 70 230 69 230 9.80e-22 91.7
MsG0780040459.01.T01 MTR_2g015890 34.058 138 82 3 109 241 2 135 1.80e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040459.01.T01 AT2G41130 62.100 219 77 5 18 233 10 225 5.12e-81 244
MsG0780040459.01.T01 AT3G56770 61.140 193 61 4 70 250 39 229 6.06e-73 222
MsG0780040459.01.T01 AT1G68810 45.729 199 96 3 57 243 151 349 2.17e-46 159
MsG0780040459.01.T01 AT3G25710 57.857 140 57 1 72 211 128 265 6.69e-44 151
MsG0780040459.01.T01 AT2G40200 41.579 190 94 4 71 245 58 245 8.11e-35 125
MsG0780040459.01.T01 AT4G38070 34.807 181 102 5 26 206 50 214 2.23e-22 92.8

Find 48 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTGTTATTTCGTCGGTTT+CGG 0.235930 7:+77892597 None:intergenic
CAACAAATTCAGAATAATAT+TGG 0.249136 7:-77893988 MsG0780040459.01.T01:CDS
TTTCGATTAGGTCGGGAATT+AGG 0.256589 7:+77892497 None:intergenic
GTAATAGTTATTGTTGTTAT+CGG 0.378138 7:+77893855 None:intergenic
GAATTCACTCAGGTCTTTGT+TGG 0.378871 7:-77892347 MsG0780040459.01.T01:CDS
GAGACTTGAGGATTTCGATT+AGG 0.380801 7:+77892485 None:intergenic
AACAAATTCAGAATAATATT+GGG 0.385638 7:-77893987 MsG0780040459.01.T01:CDS
TGTTGCATCATAGTGCTACT+AGG 0.431842 7:+77893895 None:intergenic
GTCGAAAAGGCGTCGAGGGT+TGG 0.453710 7:-77892299 MsG0780040459.01.T01:CDS
GTTGAGTATTCAAAGAATGA+AGG 0.456323 7:+77893919 None:intergenic
GCTGAAATGGCCACACTTGG+TGG 0.460387 7:-77892442 MsG0780040459.01.T01:CDS
CACTCAGGTCTTTGTTGGAT+AGG 0.462227 7:-77892342 MsG0780040459.01.T01:CDS
AGGAAGCTGAGAAGAGAAGA+AGG 0.471315 7:-77893756 MsG0780040459.01.T01:CDS
GGCAATGACATCTCCGACGA+AGG 0.483297 7:-77892565 MsG0780040459.01.T01:CDS
GTTATTTCGTCGGTTTCGGA+TGG 0.483862 7:+77892601 None:intergenic
GTAATTTCAGAAACTTCAAA+GGG 0.483898 7:+77893829 None:intergenic
TTGAGGATTTCGATTAGGTC+GGG 0.493776 7:+77892490 None:intergenic
AGTAATTTCAGAAACTTCAA+AGG 0.496460 7:+77893828 None:intergenic
CTTGAGGATTTCGATTAGGT+CGG 0.504746 7:+77892489 None:intergenic
CCTTGGAGTTGCAAGGGAGA+AGG 0.512266 7:+77893698 None:intergenic
CTTGGAGTTGCAAGGGAGAA+GGG 0.521866 7:+77893699 None:intergenic
TTCGTCGGAGATGTCATTGC+CGG 0.522101 7:+77892567 None:intergenic
TTGAGTATTCAAAGAATGAA+GGG 0.546799 7:+77893920 None:intergenic
CCTTCTCCCTTGCAACTCCA+AGG 0.549041 7:-77893698 MsG0780040459.01.T01:intron
ATAATAACCTTGGAGTTGCA+AGG 0.549750 7:+77893691 None:intergenic
AGAGCTGAAATGGCCACACT+TGG 0.551920 7:-77892445 MsG0780040459.01.T01:CDS
TCTTTGTTGGATAGGTCTAG+TGG 0.556474 7:-77892334 MsG0780040459.01.T01:CDS
AGATATGACTGTTATTTCGT+CGG 0.558305 7:+77892591 None:intergenic
GGTCTAGTGGTTGTAACGAT+AGG 0.565435 7:-77892321 MsG0780040459.01.T01:CDS
TAATAACCTTGGAGTTGCAA+GGG 0.573577 7:+77893692 None:intergenic
AAGGGAGAAGGGTACGAAGA+TGG 0.575464 7:+77893710 None:intergenic
ACGTGTTCTTCCACCAAGTG+TGG 0.575747 7:+77892432 None:intergenic
GAATATGAGTCTGCCTTCGT+CGG 0.576237 7:+77892552 None:intergenic
ATAGGTCGAAAAGGCGTCGA+GGG 0.576982 7:-77892303 MsG0780040459.01.T01:CDS
AGAGCTCTATCTTGCTGAGA+GGG 0.579252 7:+77893799 None:intergenic
GTTGTAACGATAGGTCGAAA+AGG 0.580273 7:-77892312 MsG0780040459.01.T01:CDS
AATTCAGAATAATATTGGGA+TGG 0.586813 7:-77893983 MsG0780040459.01.T01:CDS
TTTCAGAAACTTCAAAGGGG+TGG 0.587837 7:+77893833 None:intergenic
GAAATAACAGTCATATCTGC+CGG 0.595239 7:-77892586 MsG0780040459.01.T01:CDS
GGAAGCTGAGAAGAGAAGAA+GGG 0.604105 7:-77893755 MsG0780040459.01.T01:CDS
GATTAGGTCGGGAATTAGGT+CGG 0.607145 7:+77892501 None:intergenic
GAAGGGTACGAAGATGGTCA+AGG 0.633890 7:+77893716 None:intergenic
AGCTGCTTTGAAGAATCACA+AGG 0.645458 7:-77893776 MsG0780040459.01.T01:CDS
TAGAGCTCTATCTTGCTGAG+AGG 0.647696 7:+77893798 None:intergenic
ATGAAGGGTTGTTAGCAACA+AGG 0.651347 7:+77893935 None:intergenic
GATAGGTCGAAAAGGCGTCG+AGG 0.658588 7:-77892304 MsG0780040459.01.T01:CDS
TGATTGATTTCAGACAGACA+AGG 0.674472 7:-77892704 MsG0780040459.01.T01:intron
TAATTTCAGAAACTTCAAAG+GGG 0.757536 7:+77893830 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAATTCAGAATAATATT+GGG - Chr7:77892289-77892308 MsG0780040459.01.T01:CDS 15.0%
!! AATGATGAAAATAATAACCT+TGG + Chr7:77892598-77892617 None:intergenic 20.0%
!! AATTATTTGCAAATTAAGCT+AGG - Chr7:77893159-77893178 MsG0780040459.01.T01:intron 20.0%
!! ATTATTTGCAAATTAAGCTA+GGG - Chr7:77893160-77893179 MsG0780040459.01.T01:intron 20.0%
!! CAACAAATTCAGAATAATAT+TGG - Chr7:77892288-77892307 MsG0780040459.01.T01:CDS 20.0%
!! GATGTTCCAAAAAATAATTA+AGG + Chr7:77892940-77892959 None:intergenic 20.0%
!! TCATTGTTAATTATTTGTCT+TGG - Chr7:77892613-77892632 MsG0780040459.01.T01:CDS 20.0%
!!! CTAGATCCTTAATTATTTTT+TGG - Chr7:77892931-77892950 MsG0780040459.01.T01:intron 20.0%
!!! GCTTAATTTGCAAATAATTT+TGG + Chr7:77893158-77893177 None:intergenic 20.0%
!!! GTAATAGTTATTGTTGTTAT+CGG + Chr7:77892424-77892443 None:intergenic 20.0%
!!! TTGGATTACATATTTTTTGT+AGG + Chr7:77893089-77893108 None:intergenic 20.0%
! AATTCAGAATAATATTGGGA+TGG - Chr7:77892293-77892312 MsG0780040459.01.T01:CDS 25.0%
! AGTAATTTCAGAAACTTCAA+AGG + Chr7:77892451-77892470 None:intergenic 25.0%
! CTTAATTACTAAAGTGAGAA+AGG + Chr7:77892829-77892848 None:intergenic 25.0%
! GTAATTTCAGAAACTTCAAA+GGG + Chr7:77892450-77892469 None:intergenic 25.0%
! TAATTTCAGAAACTTCAAAG+GGG + Chr7:77892449-77892468 None:intergenic 25.0%
! TAGTCATGTATAAAACTTGA+TGG + Chr7:77893249-77893268 None:intergenic 25.0%
! TTGAGTATTCAAAGAATGAA+GGG + Chr7:77892359-77892378 None:intergenic 25.0%
!! TACATGTTTTCACTCTATTT+TGG + Chr7:77892775-77892794 None:intergenic 25.0%
!! TCAATTTTACAAAACCTACA+AGG - Chr7:77893367-77893386 MsG0780040459.01.T01:intron 25.0%
!! TTTTGTTCTTTTGTTCTCAA+AGG - Chr7:77892673-77892692 MsG0780040459.01.T01:CDS 25.0%
!!! TTGTAGGTTTTGTAAAATTG+AGG + Chr7:77893368-77893387 None:intergenic 25.0%
AAACCATTGTGATTTAGAAG+AGG + Chr7:77893395-77893414 None:intergenic 30.0%
GTTGAGTATTCAAAGAATGA+AGG + Chr7:77892360-77892379 None:intergenic 30.0%
TGATGAGTGATTAAGTTCAT+TGG + Chr7:77893108-77893127 None:intergenic 30.0%
! ATTTTCTTCAGAATTCACTC+AGG - Chr7:77893919-77893938 MsG0780040459.01.T01:CDS 30.0%
! CTTTAGTAATTAAGTTGCCA+TGG - Chr7:77892835-77892854 MsG0780040459.01.T01:intron 30.0%
!! AGATATGACTGTTATTTCGT+CGG + Chr7:77893688-77893707 None:intergenic 30.0%
!! TCATTTTTGTCTCAAAACCT+AGG + Chr7:77893472-77893491 None:intergenic 30.0%
!! TGTTTTCACTCTATTTTGGA+TGG + Chr7:77892771-77892790 None:intergenic 30.0%
AAAAACTCTCAGAGCTGAAA+TGG - Chr7:77893821-77893840 MsG0780040459.01.T01:CDS 35.0%
ACACACACAATTATCTTTGC+TGG + Chr7:77892714-77892733 None:intergenic 35.0%
ATAATAACCTTGGAGTTGCA+AGG + Chr7:77892588-77892607 None:intergenic 35.0%
CTAGTACATGTTTGCATATG+TGG + Chr7:77893430-77893449 None:intergenic 35.0%
GAAATAACAGTCATATCTGC+CGG - Chr7:77893690-77893709 MsG0780040459.01.T01:intron 35.0%
TAATAACCTTGGAGTTGCAA+GGG + Chr7:77892587-77892606 None:intergenic 35.0%
TCAATCTTGTCACAAAACCT+AGG + Chr7:77893509-77893528 None:intergenic 35.0%
TCTTATGAGTTTAGCACAAG+TGG - Chr7:77893004-77893023 MsG0780040459.01.T01:intron 35.0%
TGATGAAGAAAGCAAAACCT+AGG - Chr7:77893489-77893508 MsG0780040459.01.T01:intron 35.0%
! AGGCATATTTTCACTCATTC+TGG + Chr7:77892809-77892828 None:intergenic 35.0%
! AGTATCGAACTCAAAACATC+CGG + Chr7:77893064-77893083 None:intergenic 35.0%
! GTAAAGTACTACATGTTCCA+TGG + Chr7:77892855-77892874 None:intergenic 35.0%
! TGATTGATTTCAGACAGACA+AGG - Chr7:77893572-77893591 MsG0780040459.01.T01:intron 35.0%
!!! TTTCAGCTCTGAGAGTTTTT+AGG + Chr7:77893821-77893840 None:intergenic 35.0%
!!! TTTTTAGGTGAAGAGACTTG+AGG + Chr7:77893806-77893825 None:intergenic 35.0%
ATGAAGGGTTGTTAGCAACA+AGG + Chr7:77892344-77892363 None:intergenic 40.0%
ATGAGTTTAGCACAAGTGGT+CGG - Chr7:77893008-77893027 MsG0780040459.01.T01:intron 40.0%
ATTTAGAAGAGGCTCCTTGT+AGG + Chr7:77893384-77893403 None:intergenic 40.0%
CTTGAGGATTTCGATTAGGT+CGG + Chr7:77893790-77893809 None:intergenic 40.0%
GAATTCACTCAGGTCTTTGT+TGG - Chr7:77893929-77893948 MsG0780040459.01.T01:CDS 40.0%
GAGACTTGAGGATTTCGATT+AGG + Chr7:77893794-77893813 None:intergenic 40.0%
GAGCCTCTTCTAAATCACAA+TGG - Chr7:77893389-77893408 MsG0780040459.01.T01:intron 40.0%
GTTGTAACGATAGGTCGAAA+AGG - Chr7:77893964-77893983 MsG0780040459.01.T01:CDS 40.0%
TCTTTGTTGGATAGGTCTAG+TGG - Chr7:77893942-77893961 MsG0780040459.01.T01:CDS 40.0%
TGATGAAGAAAGCAAAGCCT+AGG - Chr7:77893452-77893471 MsG0780040459.01.T01:intron 40.0%
TTAAGCTAGGGAAGTCACTT+AGG - Chr7:77893172-77893191 MsG0780040459.01.T01:intron 40.0%
TTGAGGATTTCGATTAGGTC+GGG + Chr7:77893789-77893808 None:intergenic 40.0%
TTTCAGAAACTTCAAAGGGG+TGG + Chr7:77892446-77892465 None:intergenic 40.0%
TTTCGATTAGGTCGGGAATT+AGG + Chr7:77893782-77893801 None:intergenic 40.0%
! AGGGAAGTCACTTAGGTTTT+AGG - Chr7:77893179-77893198 MsG0780040459.01.T01:intron 40.0%
! TAGCTTTTGCAAAATCGTGG+TGG + Chr7:77893340-77893359 None:intergenic 40.0%
! TCGTAGCTTTTGCAAAATCG+TGG + Chr7:77893343-77893362 None:intergenic 40.0%
! TGTCTGAAGTGGTGTATATC+CGG - Chr7:77893042-77893061 MsG0780040459.01.T01:intron 40.0%
! TGTTGCATCATAGTGCTACT+AGG + Chr7:77892384-77892403 None:intergenic 40.0%
!! AGCTGCTTTGAAGAATCACA+AGG - Chr7:77892500-77892519 MsG0780040459.01.T01:CDS 40.0%
!! GACTGTTATTTCGTCGGTTT+CGG + Chr7:77893682-77893701 None:intergenic 40.0%
AGAGCTCTATCTTGCTGAGA+GGG + Chr7:77892480-77892499 None:intergenic 45.0%
AGGAAGCTGAGAAGAGAAGA+AGG - Chr7:77892520-77892539 MsG0780040459.01.T01:CDS 45.0%
CACTCAGGTCTTTGTTGGAT+AGG - Chr7:77893934-77893953 MsG0780040459.01.T01:CDS 45.0%
GAATATGAGTCTGCCTTCGT+CGG + Chr7:77893727-77893746 None:intergenic 45.0%
GATTAGGTCGGGAATTAGGT+CGG + Chr7:77893778-77893797 None:intergenic 45.0%
GGAAGCTGAGAAGAGAAGAA+GGG - Chr7:77892521-77892540 MsG0780040459.01.T01:CDS 45.0%
GGTCTAGTGGTTGTAACGAT+AGG - Chr7:77893955-77893974 MsG0780040459.01.T01:CDS 45.0%
TAGAGCTCTATCTTGCTGAG+AGG + Chr7:77892481-77892500 None:intergenic 45.0%
TGCGTAATGTGTGTCTGAAG+TGG - Chr7:77893031-77893050 MsG0780040459.01.T01:intron 45.0%
!! GTTATTTCGTCGGTTTCGGA+TGG + Chr7:77893678-77893697 None:intergenic 45.0%
!!! TTTTATTTTTTTACTATTTA+AGG + Chr7:77893135-77893154 None:intergenic 5.0%
ACGTGTTCTTCCACCAAGTG+TGG + Chr7:77893847-77893866 None:intergenic 50.0%
AGAGCTGAAATGGCCACACT+TGG - Chr7:77893831-77893850 MsG0780040459.01.T01:CDS 50.0%
CTTGGAGTTGCAAGGGAGAA+GGG + Chr7:77892580-77892599 None:intergenic 50.0%
TTCGTCGGAGATGTCATTGC+CGG + Chr7:77893712-77893731 None:intergenic 50.0%
! AAGGGAGAAGGGTACGAAGA+TGG + Chr7:77892569-77892588 None:intergenic 50.0%
! GAAGGGTACGAAGATGGTCA+AGG + Chr7:77892563-77892582 None:intergenic 50.0%
!! ATAGGTCGAAAAGGCGTCGA+GGG - Chr7:77893973-77893992 MsG0780040459.01.T01:CDS 50.0%
CCTTCTCCCTTGCAACTCCA+AGG - Chr7:77892578-77892597 MsG0780040459.01.T01:CDS 55.0%
CCTTGGAGTTGCAAGGGAGA+AGG + Chr7:77892581-77892600 None:intergenic 55.0%
GCTGAAATGGCCACACTTGG+TGG - Chr7:77893834-77893853 MsG0780040459.01.T01:CDS 55.0%
GGCAATGACATCTCCGACGA+AGG - Chr7:77893711-77893730 MsG0780040459.01.T01:CDS 55.0%
!! GATAGGTCGAAAAGGCGTCG+AGG - Chr7:77893972-77893991 MsG0780040459.01.T01:CDS 55.0%
!! GTCGAAAAGGCGTCGAGGGT+TGG - Chr7:77893977-77893996 MsG0780040459.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 77892279 77894019 77892279 ID=MsG0780040459.01;Name=MsG0780040459.01
Chr7 mRNA 77892279 77894019 77892279 ID=MsG0780040459.01.T01;Parent=MsG0780040459.01;Name=MsG0780040459.01.T01;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|1|1|2|0|251
Chr7 exon 77893699 77894019 77893699 ID=MsG0780040459.01.T01:exon:15016;Parent=MsG0780040459.01.T01
Chr7 exon 77892279 77892713 77892279 ID=MsG0780040459.01.T01:exon:15015;Parent=MsG0780040459.01.T01
Chr7 CDS 77893699 77894019 77893699 ID=MsG0780040459.01.T01:cds;Parent=MsG0780040459.01.T01
Chr7 CDS 77892279 77892713 77892279 ID=MsG0780040459.01.T01:cds;Parent=MsG0780040459.01.T01
Gene Sequence

>MsG0780040459.01.T01

ATGCTTCAGCAACAAATTCAGAATAATATTGGGATGGAAAACAACTCTGAACTCTTTCAATTCCTTGTTGCTAACAACCCTTCATTCTTTGAATACTCAACACCTAGTAGCACTATGATGCAACAAAGTTTCTGCAGTTCTTCCGATAACAACAATAACTATTACCACCCCTTTGAAGTTTCTGAAATTACTGATACACCCTCTCAGCAAGATAGAGCTCTAGCTGCTTTGAAGAATCACAAGGAAGCTGAGAAGAGAAGAAGGGAAAGAATTAACTCTCACCTTGACCATCTTCGTACCCTTCTCCCTTGCAACTCCAAGACAGACAAGGCTTCACTTCTAGCAAAAGTAGTAGAACGTGTGAAAGAATTAAAGCAACAAACATCTCAAATCACACAACTCGAAACTGTTCCATCCGAAACCGACGAAATAACAGTCATATCTGCCGGCAATGACATCTCCGACGAAGGCAGACTCATATTCAAAGCTTCATTATGCTGCGAAGATCGCTCCGACCTAATTCCCGACCTAATCGAAATCCTCAAGTCTCTTCACCTAAAAACTCTCAGAGCTGAAATGGCCACACTTGGTGGAAGAACACGTAACGTTCTTGTTGTTGCTGCTGAGAAAGAACATAATAGCATTGAATCTATTCATTTTCTTCAGAATTCACTCAGGTCTTTGTTGGATAGGTCTAGTGGTTGTAACGATAGGTCGAAAAGGCGTCGAGGGTTGGATAGAAGAATGATGCCTTAA

Protein sequence

>MsG0780040459.01.T01

MLQQQIQNNIGMENNSELFQFLVANNPSFFEYSTPSSTMMQQSFCSSSDNNNNYYHPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTDKASLLAKVVERVKELKQQTSQITQLETVPSETDEITVISAGNDISDEGRLIFKASLCCEDRSDLIPDLIEILKSLHLKTLRAEMATLGGRTRNVLVVAAEKEHNSIESIHFLQNSLRSLLDRSSGCNDRSKRRRGLDRRMMP*