AlfalfaGEDB | MsG0780036733.01

M. sativa cultivar ZhongmuNo.1 / MsG0780036733.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036733.01.T02	XP_013441776.1	92	100	6	2	1	99	37	135	6.74E-42	144
MsG0780036733.01.T01	XP_013441776.1	91.912	136	7	3	1	133	1	135	3.09E-59	189

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036733.01.T01	O81775	40.8	125	59	4	1	115	1	120	1.15E-22	90.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036733.01.T01	I3SK48	91.912	136	7	3	1	133	1	135	1.48e-59	189
MsG0780036733.01.T02	I3SK48	92.000	100	6	2	1	99	37	135	3.22e-42	144

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005090.01	MsG0780036733.01	0.811324	7.409756e-51	4.572684e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036733.01.T02	MTR_0542s0020	92.000	100	6	2	1	99	37	135	8.16e-46	144
MsG0780036733.01.T02	MTR_7g018090	44.118	68	35	2	1	67	40	105	1.46e-13	61.6
MsG0780036733.01.T01	MTR_0542s0020	91.912	136	7	3	1	133	1	135	3.75e-63	189
MsG0780036733.01.T01	MTR_7g018090	44.860	107	51	3	1	101	1	105	3.09e-25	92.8
MsG0780036733.01.T01	MTR_4g129020	46.835	79	41	1	21	98	25	103	4.88e-19	78.6
MsG0780036733.01.T01	MTR_6g016480	43.750	80	39	2	23	101	14	88	3.63e-18	74.3
MsG0780036733.01.T01	MTR_1g013410	43.023	86	48	1	20	104	24	109	3.61e-17	72.8
MsG0780036733.01.T01	MTR_3g105690	41.509	106	56	1	7	106	14	119	3.65e-17	73.2
MsG0780036733.01.T01	MTR_3g105690	41.509	106	56	1	7	106	14	119	6.10e-17	72.8
MsG0780036733.01.T01	MTR_4g110130	40.000	80	47	1	21	100	44	122	5.34e-13	62.0
MsG0780036733.01.T01	MTR_1g013430	37.363	91	46	2	20	104	24	109	4.60e-12	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036733.01.T02	AT4G31730	41.463	82	42	2	1	81	44	120	3.91e-12	58.9
MsG0780036733.01.T02	AT4G25760	43.750	80	42	2	1	79	42	119	4.94e-11	55.5
MsG0780036733.01.T01	AT4G31730	40.800	125	59	4	1	115	1	120	1.17e-23	90.1
MsG0780036733.01.T01	AT4G25760	48.936	94	45	2	21	113	28	119	5.64e-22	85.1
MsG0780036733.01.T01	AT2G24762	43.269	104	53	2	18	120	30	128	2.09e-20	81.6
MsG0780036733.01.T01	AT5G57685	45.918	98	42	3	21	112	28	120	4.59e-19	78.2
MsG0780036733.01.T01	AT5G24920	41.975	81	42	1	21	101	28	103	6.56e-13	61.6

Find 20 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTAATCTTCTCTATGTTCT+TGG	0.218840	7:+12707469	None:intergenic
TCTCCAATTCCATATCTATT+TGG	0.228950	7:-12707617	MsG0780036733.01.T01:CDS
AAAGAAGTAGTGATAATAAT+TGG	0.275831	7:+12707386	None:intergenic
GCCATAATCACAAGTACTTC+TGG	0.351204	7:+12707440	None:intergenic
ATTAGTTAATGTTTGGTCAT+TGG	0.359783	7:+12707307	None:intergenic
TTGCCATATCTTAATCTCAT+TGG	0.363415	7:+12707640	None:intergenic
TAATGTTTGGTCATTGGTTA+AGG	0.364149	7:+12707313	None:intergenic
CAACTTAATTAGTTAATGTT+TGG	0.367551	7:+12707300	None:intergenic
CCTCCAAATAGATATGGAAT+TGG	0.395109	7:+12707614	None:intergenic
GCCAGAAGTACTTGTGATTA+TGG	0.399181	7:-12707441	MsG0780036733.01.T01:CDS
GCTAAGCCTCCAAATAGATA+TGG	0.402398	7:+12707608	None:intergenic
AGTAGTGATAATAATTGGTT+TGG	0.410163	7:+12707391	None:intergenic
ATTGGTTTGGCTATGTAAGT+TGG	0.431103	7:+12707404	None:intergenic
TTCAGATGAAGAAATGAAAC+AGG	0.432931	7:-12707498	MsG0780036733.01.T01:CDS
CCAATTCCATATCTATTTGG+AGG	0.435839	7:-12707614	MsG0780036733.01.T01:CDS
TCTTTACCTTACTGCACTTG+TGG	0.450831	7:-12707368	MsG0780036733.01.T01:CDS
AAACCAATGAGATTAAGATA+TGG	0.476091	7:-12707643	MsG0780036733.01.T01:CDS
GAAGTACTTGTGATTATGGC+TGG	0.486766	7:-12707437	MsG0780036733.01.T01:CDS
GATGATGATAAAGTGTTGAT+TGG	0.502596	7:+12707677	None:intergenic
TCAGCTCCACAAGTGCAGTA+AGG	0.569478	7:+12707362	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGAAGTAGTGATAATAAT+TGG	+	Chr7:12707604-12707623	None:intergenic	20.0%
!!!	AATTTTAATTTCAGTTGCAT+TGG	-	Chr7:12707411-12707430	MsG0780036733.01.T01:CDS	20.0%
!	AAACCAATGAGATTAAGATA+TGG	-	Chr7:12707344-12707363	MsG0780036733.01.T01:CDS	25.0%
!	AGTAGTGATAATAATTGGTT+TGG	+	Chr7:12707599-12707618	None:intergenic	25.0%
!	ATTAATCTTCTCTATGTTCT+TGG	+	Chr7:12707521-12707540	None:intergenic	25.0%
!	ATTAGTTAATGTTTGGTCAT+TGG	+	Chr7:12707683-12707702	None:intergenic	25.0%
	TCTCCAATTCCATATCTATT+TGG	-	Chr7:12707370-12707389	MsG0780036733.01.T01:CDS	30.0%
	TTCAGATGAAGAAATGAAAC+AGG	-	Chr7:12707489-12707508	MsG0780036733.01.T01:CDS	30.0%
	TTGCCATATCTTAATCTCAT+TGG	+	Chr7:12707350-12707369	None:intergenic	30.0%
!	TAATGTTTGGTCATTGGTTA+AGG	+	Chr7:12707677-12707696	None:intergenic	30.0%
!!	GATGATGATAAAGTGTTGAT+TGG	+	Chr7:12707313-12707332	None:intergenic	30.0%
	CCAATTCCATATCTATTTGG+AGG	-	Chr7:12707373-12707392	MsG0780036733.01.T01:CDS	35.0%
	CCTCCAAATAGATATGGAAT+TGG	+	Chr7:12707376-12707395	None:intergenic	35.0%
!!	ATTGGTTTGGCTATGTAAGT+TGG	+	Chr7:12707586-12707605	None:intergenic	35.0%
!!!	AGAAGAACCACGTTTTTTGT+AGG	+	Chr7:12707452-12707471	None:intergenic	35.0%
!!!	GTTTTTTGTAGGAGCATACA+AGG	+	Chr7:12707441-12707460	None:intergenic	35.0%
	GCCATAATCACAAGTACTTC+TGG	+	Chr7:12707550-12707569	None:intergenic	40.0%
	GCTAAGCCTCCAAATAGATA+TGG	+	Chr7:12707382-12707401	None:intergenic	40.0%
	GTATGCTCCTACAAAAAACG+TGG	-	Chr7:12707442-12707461	MsG0780036733.01.T01:CDS	40.0%
	TCTTTACCTTACTGCACTTG+TGG	-	Chr7:12707619-12707638	MsG0780036733.01.T01:CDS	40.0%
!	GAAGTACTTGTGATTATGGC+TGG	-	Chr7:12707550-12707569	MsG0780036733.01.T01:CDS	40.0%
!!	GCCAGAAGTACTTGTGATTA+TGG	-	Chr7:12707546-12707565	MsG0780036733.01.T01:CDS	40.0%
	TCAGCTCCACAAGTGCAGTA+AGG	+	Chr7:12707628-12707647	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	12707304	12707705	12707304	ID=MsG0780036733.01;Name=MsG0780036733.01
Chr7	mRNA	12707304	12707705	12707304	ID=MsG0780036733.01.T01;Parent=MsG0780036733.01;Name=MsG0780036733.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|133
Chr7	exon	12707304	12707705	12707304	ID=MsG0780036733.01.T01:exon:2630;Parent=MsG0780036733.01.T01
Chr7	CDS	12707304	12707705	12707304	ID=MsG0780036733.01.T01:cds;Parent=MsG0780036733.01.T01
Chr7	mRNA	12707304	12707603	12707304	ID=MsG0780036733.01.T02;Parent=MsG0780036733.01;Name=MsG0780036733.01.T02;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|99
Chr7	exon	12707304	12707603	12707304	ID=MsG0780036733.01.T02:exon:2631;Parent=MsG0780036733.01.T02
Chr7	CDS	12707304	12707603	12707304	ID=MsG0780036733.01.T02:cds;Parent=MsG0780036733.01.T02

Gene Sequence

>MsG0780036733.01.T02

ATGCTAATTTTAATTTCAGTTGCATTGGTGATCCTTGTATGCTCCTACAAAAAACGTGGTTCTTCTCAATCATCATCAAATAATTCAGATGAAGAAATGAAACAGGTAATGTCCAAGAACATAGAGAAGATTAATTCTGAGCCAGAAGTACTTGTGATTATGGCTGGAGAAGATAAACCAACTTACATAGCCAAACCAATTATTATCACTACTTCTTTACCTTACTGCACTTGTGGAGCTGAATCAACTTCAACAATATCTTCATCAACCTTAACCAATGACCAAACATTAACTAATTAA

>MsG0780036733.01.T01

ATGAGGCCAATCAACACTTTATCATCATCATCAACAACTCAAACCAATGAGATTAAGATATGGCAATCTCCAATTCCATATCTATTTGGAGGCTTAGCTATAATGCTAATTTTAATTTCAGTTGCATTGGTGATCCTTGTATGCTCCTACAAAAAACGTGGTTCTTCTCAATCATCATCAAATAATTCAGATGAAGAAATGAAACAGGTAATGTCCAAGAACATAGAGAAGATTAATTCTGAGCCAGAAGTACTTGTGATTATGGCTGGAGAAGATAAACCAACTTACATAGCCAAACCAATTATTATCACTACTTCTTTACCTTACTGCACTTGTGGAGCTGAATCAACTTCAACAATATCTTCATCAACCTTAACCAATGACCAAACATTAACTAATTAA

Protein sequence

>MsG0780036733.01.T02

MLILISVALVILVCSYKKRGSSQSSSNNSDEEMKQVMSKNIEKINSEPEVLVIMAGEDKPTYIAKPIIITTSLPYCTCGAESTSTISSSTLTNDQTLTN*

>MsG0780036733.01.T01

MRPINTLSSSSTTQTNEIKIWQSPIPYLFGGLAIMLILISVALVILVCSYKKRGSSQSSSNNSDEEMKQVMSKNIEKINSEPEVLVIMAGEDKPTYIAKPIIITTSLPYCTCGAESTSTISSSTLTNDQTLTN*