Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036760.01.T01 | XP_024632840.1 | 76.119 | 67 | 8 | 2 | 1 | 66 | 37 | 96 | 1.80E-25 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036760.01.T01 | A0A396JWR6 | 76.119 | 67 | 8 | 2 | 1 | 66 | 37 | 96 | 8.59e-26 | 100 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007331.01 | MsG0780036760.01 | 0.836751 | 7.786790e-57 | 9.906272e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036760.01.T01 | MTR_5g077900 | 76.119 | 67 | 8 | 2 | 1 | 66 | 165 | 224 | 8.53e-28 | 99.8 |
| MsG0780036760.01.T01 | MTR_0057s0200 | 74.242 | 66 | 9 | 2 | 4 | 68 | 1 | 59 | 2.69e-27 | 93.6 |
| MsG0780036760.01.T01 | MTR_0002s1270 | 76.119 | 67 | 8 | 2 | 1 | 66 | 339 | 398 | 2.71e-27 | 101 |
| MsG0780036760.01.T01 | MTR_7g046380 | 81.481 | 54 | 3 | 1 | 1 | 54 | 347 | 393 | 4.91e-25 | 95.5 |
| MsG0780036760.01.T01 | MTR_4g066320 | 65.714 | 70 | 15 | 3 | 1 | 68 | 59 | 121 | 5.11e-25 | 89.7 |
| MsG0780036760.01.T01 | MTR_4g066300 | 62.745 | 51 | 19 | 0 | 2 | 52 | 7 | 57 | 1.17e-16 | 67.0 |
| MsG0780036760.01.T01 | MTR_3g028430 | 50.820 | 61 | 13 | 2 | 9 | 68 | 15 | 59 | 4.80e-11 | 52.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCCATTAGGTTGCTTTCT+TGG | 0.248489 | 7:+13316100 | None:intergenic |
| TATAAGTCATCATCAGTTAA+TGG | 0.351177 | 7:+13315889 | None:intergenic |
| TGAAAAGAGTTAATTACTAA+TGG | 0.377517 | 7:+13315452 | None:intergenic |
| TCTTGACTTGTATAAGGCTT+AGG | 0.381448 | 7:-13315839 | MsG0780036760.01.T01:exon |
| TATCCACAATCATTGCCATT+AGG | 0.411955 | 7:+13316088 | None:intergenic |
| TTTACAATATAGCTAGAAAG+AGG | 0.472041 | 7:+13315494 | None:intergenic |
| CTGCTAATATTACTAAGTCT+TGG | 0.472781 | 7:-13316033 | MsG0780036760.01.T01:CDS |
| TAGCCAAGAAAGCAACCTAA+TGG | 0.495668 | 7:-13316103 | MsG0780036760.01.T01:CDS |
| TATAAGGCTTAGGATTTACA+TGG | 0.496621 | 7:-13315829 | MsG0780036760.01.T01:intron |
| CAACCTAATGGCAATGATTG+TGG | 0.497368 | 7:-13316091 | MsG0780036760.01.T01:CDS |
| TCATCATCAGTTAATGGCGT+TGG | 0.497730 | 7:+13315895 | None:intergenic |
| GTCAAGAAAACAAGTGCCAT+TGG | 0.523992 | 7:+13315855 | None:intergenic |
| CTCATCTCAGTCGTAAGCAT+CGG | 0.575955 | 7:+13315626 | None:intergenic |
| TAATATTACTAAGTCTTGGA+TGG | 0.604101 | 7:-13316029 | MsG0780036760.01.T01:CDS |
| TTATATGATTGCGAAACCAA+TGG | 0.604628 | 7:-13315871 | MsG0780036760.01.T01:CDS |
| TTACGATGATGATTCTGCTG+TGG | 0.615064 | 7:-13315569 | MsG0780036760.01.T01:three_prime_UTR |
| TATTACTAAGTCTTGGATGG+AGG | 0.643493 | 7:-13316026 | MsG0780036760.01.T01:CDS |
| AGCTGAAAATGATTATGATG+TGG | 0.702699 | 7:-13315665 | MsG0780036760.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTGTTTCTAAATTGT+TGG | - | Chr7:13316006-13316025 | MsG0780036760.01.T01:CDS | 15.0% |
| !! | TGAAAAGAGTTAATTACTAA+TGG | + | Chr7:13316100-13316119 | None:intergenic | 20.0% |
| ! | CAAATGTCAAATTATGAGAT+TGG | + | Chr7:13315758-13315777 | None:intergenic | 25.0% |
| ! | TAATATTACTAAGTCTTGGA+TGG | - | Chr7:13315520-13315539 | MsG0780036760.01.T01:three_prime_UTR | 25.0% |
| ! | TATAAGTCATCATCAGTTAA+TGG | + | Chr7:13315663-13315682 | None:intergenic | 25.0% |
| ! | TATATTAACCTACACATTGT+AGG | - | Chr7:13315621-13315640 | MsG0780036760.01.T01:three_prime_UTR | 25.0% |
| ! | TTTACAATATAGCTAGAAAG+AGG | + | Chr7:13316058-13316077 | None:intergenic | 25.0% |
| !!! | TTGTTTTCTTGACTTGTATA+AGG | - | Chr7:13315704-13315723 | MsG0780036760.01.T01:intron | 25.0% |
| AGCTGAAAATGATTATGATG+TGG | - | Chr7:13315884-13315903 | MsG0780036760.01.T01:CDS | 30.0% | |
| CTGCTAATATTACTAAGTCT+TGG | - | Chr7:13315516-13315535 | MsG0780036760.01.T01:three_prime_UTR | 30.0% | |
| TATAAGGCTTAGGATTTACA+TGG | - | Chr7:13315720-13315739 | MsG0780036760.01.T01:intron | 30.0% | |
| TTATATGATTGCGAAACCAA+TGG | - | Chr7:13315678-13315697 | MsG0780036760.01.T01:intron | 30.0% | |
| ! | GTAGAGAGTAGAATTTTGAA+CGG | - | Chr7:13315777-13315796 | MsG0780036760.01.T01:intron | 30.0% |
| CTTGTATCTGTCTTAATGTG+TGG | - | Chr7:13315801-13315820 | MsG0780036760.01.T01:intron | 35.0% | |
| TATCCACAATCATTGCCATT+AGG | + | Chr7:13315464-13315483 | None:intergenic | 35.0% | |
| TATTACTAAGTCTTGGATGG+AGG | - | Chr7:13315523-13315542 | MsG0780036760.01.T01:three_prime_UTR | 35.0% | |
| !! | TCTTGACTTGTATAAGGCTT+AGG | - | Chr7:13315710-13315729 | MsG0780036760.01.T01:intron | 35.0% |
| CAACCTAATGGCAATGATTG+TGG | - | Chr7:13315458-13315477 | MsG0780036760.01.T01:three_prime_UTR | 40.0% | |
| CATGAAGACCTACAATGTGT+AGG | + | Chr7:13315632-13315651 | None:intergenic | 40.0% | |
| GTATCTGTCTTAATGTGTGG+TGG | - | Chr7:13315804-13315823 | MsG0780036760.01.T01:intron | 40.0% | |
| GTCAAGAAAACAAGTGCCAT+TGG | + | Chr7:13315697-13315716 | None:intergenic | 40.0% | |
| TCATCATCAGTTAATGGCGT+TGG | + | Chr7:13315657-13315676 | None:intergenic | 40.0% | |
| TTACGATGATGATTCTGCTG+TGG | - | Chr7:13315980-13315999 | MsG0780036760.01.T01:intron | 40.0% | |
| ! | GTCTTAATGTGTGGTGGTTT+TGG | - | Chr7:13315810-13315829 | MsG0780036760.01.T01:intron | 40.0% |
| ! | TTGCCATTAGGTTGCTTTCT+TGG | + | Chr7:13315452-13315471 | None:intergenic | 40.0% |
| CTCATCTCAGTCGTAAGCAT+CGG | + | Chr7:13315926-13315945 | None:intergenic | 45.0% | |
| !! | TATAAGTAATTAATATAATA+AGG | + | Chr7:13315605-13315624 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 13315449 | 13316122 | 13315449 | ID=MsG0780036760.01;Name=MsG0780036760.01 |
| Chr7 | mRNA | 13315449 | 13316122 | 13315449 | ID=MsG0780036760.01.T01;Parent=MsG0780036760.01;Name=MsG0780036760.01.T01;_AED=0.33;_eAED=0.33;_QI=0|0.5|0.66|1|0|0|3|247|68 |
| Chr7 | exon | 13315449 | 13315685 | 13315449 | ID=MsG0780036760.01.T01:exon:1951;Parent=MsG0780036760.01.T01 |
| Chr7 | exon | 13315830 | 13315928 | 13315830 | ID=MsG0780036760.01.T01:exon:1950;Parent=MsG0780036760.01.T01 |
| Chr7 | exon | 13316005 | 13316122 | 13316005 | ID=MsG0780036760.01.T01:exon:1949;Parent=MsG0780036760.01.T01 |
| Chr7 | CDS | 13316005 | 13316122 | 13316005 | ID=MsG0780036760.01.T01:cds;Parent=MsG0780036760.01.T01 |
| Chr7 | CDS | 13315840 | 13315928 | 13315840 | ID=MsG0780036760.01.T01:cds;Parent=MsG0780036760.01.T01 |
| Chr7 | three_prime_UTR | 13315830 | 13315839 | 13315830 | ID=MsG0780036760.01.T01:three_prime_utr;Parent=MsG0780036760.01.T01 |
| Chr7 | three_prime_UTR | 13315449 | 13315685 | 13315449 | ID=MsG0780036760.01.T01:three_prime_utr;Parent=MsG0780036760.01.T01 |
| Gene Sequence |
| Protein sequence |
