Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036837.01.T01 | ACL97384.1 | 97.417 | 271 | 7 | 0 | 1 | 271 | 1 | 271 | 3.96E-173 | 524 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036837.01.T01 | P10978 | 35.766 | 274 | 150 | 9 | 2 | 259 | 3 | 266 | 2.45E-42 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036837.01.T01 | B8YLY4 | 97.417 | 271 | 7 | 0 | 1 | 271 | 1 | 271 | 1.89e-173 | 524 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002185.01 | MsG0780036837.01 | 0.800415 | 1.456547e-48 | 6.778423e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036837.01.T01 | MTR_7g069120 | 80.303 | 66 | 13 | 0 | 1 | 66 | 1 | 66 | 1.60e-28 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 60 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGACAGCAAAGGAGATT+TGG | 0.265118 | 7:-14813007 | MsG0780036837.01.T01:CDS |
| TTCTTCCTTGTTCTTGCGCC+TGG | 0.331838 | 7:+14812683 | None:intergenic |
| GAAGACAGCAAAGGAGATTT+GGG | 0.368868 | 7:-14813006 | MsG0780036837.01.T01:CDS |
| TCGTCTTCCGCCTTTAGAAC+TGG | 0.372441 | 7:+14812390 | None:intergenic |
| TGCCATTGCAAGGTGCAAAT+TGG | 0.377007 | 7:+14813058 | None:intergenic |
| TGCGCCTGGATTCTTCTTCA+AGG | 0.378064 | 7:+14812697 | None:intergenic |
| CCCTGTGGAAATTGAAGATA+AGG | 0.385271 | 7:-14813178 | MsG0780036837.01.T01:CDS |
| TGGTTGCTGCTTCGCTATAC+AGG | 0.396558 | 7:+14812410 | None:intergenic |
| GAAGACGACTCAACGATGTC+TGG | 0.401086 | 7:-14812375 | MsG0780036837.01.T01:CDS |
| CCCTTATCTTCAATTTCCAC+AGG | 0.443822 | 7:+14813177 | None:intergenic |
| TGACGAAAAGTGGAAAGAAA+TGG | 0.447767 | 7:-14813096 | MsG0780036837.01.T01:CDS |
| TTGAGAAAATAGCGTATTCA+AGG | 0.448659 | 7:+14812869 | None:intergenic |
| TTGAGATAGAGAAGTTCAAC+GGG | 0.451269 | 7:-14813211 | MsG0780036837.01.T01:CDS |
| CAGATATCACTGACGAAAAG+TGG | 0.458224 | 7:-14813106 | MsG0780036837.01.T01:CDS |
| GTCTGTCATCTATTGCGTTT+AGG | 0.458864 | 7:+14813132 | None:intergenic |
| ATGAGTTGATCATACGAATC+TGG | 0.459760 | 7:+14812783 | None:intergenic |
| TGTTGGAATATCAAGAAGAA+TGG | 0.493476 | 7:-14812498 | MsG0780036837.01.T01:CDS |
| AATATCAAATGCTACGGTTG+TGG | 0.504361 | 7:-14812546 | MsG0780036837.01.T01:CDS |
| CATGAGAGGGCACGTAAAGA+AGG | 0.510498 | 7:-14812523 | MsG0780036837.01.T01:CDS |
| ACTCAACGATGTCTGGATCA+TGG | 0.519276 | 7:-14812368 | MsG0780036837.01.T01:CDS |
| AGAATCCAGGCGCAAGAACA+AGG | 0.530445 | 7:-14812688 | MsG0780036837.01.T01:CDS |
| GAAGCAGCAACCAGTTCTAA+AGG | 0.533872 | 7:-14812400 | MsG0780036837.01.T01:CDS |
| CCTGTGGAAATTGAAGATAA+GGG | 0.535816 | 7:-14813177 | MsG0780036837.01.T01:CDS |
| TCTGAAGCTTCAACATCTCA+AGG | 0.539373 | 7:-14812465 | MsG0780036837.01.T01:CDS |
| AGGCTCTACACTCTTCGAAT+GGG | 0.543009 | 7:-14812924 | MsG0780036837.01.T01:CDS |
| GCACCTTGCAATGGCAGACT+CGG | 0.544274 | 7:-14813051 | MsG0780036837.01.T01:CDS |
| CTTCACTTTGATGATGTCGC+CGG | 0.544351 | 7:-14812726 | MsG0780036837.01.T01:CDS |
| GGCGCAAGAACAAGGAAGAA+AGG | 0.545132 | 7:-14812680 | MsG0780036837.01.T01:CDS |
| TCTTTAAGAATATTCTGTTG+TGG | 0.545908 | 7:+14812949 | None:intergenic |
| TTCTTCTTCAAGGATTGCAC+CGG | 0.552534 | 7:+14812707 | None:intergenic |
| TGCTACGGTTGTGGCATGAG+AGG | 0.552981 | 7:-14812537 | MsG0780036837.01.T01:CDS |
| CAATCCTTGAAGAAGAATCC+AGG | 0.555912 | 7:-14812701 | MsG0780036837.01.T01:CDS |
| GGCACGTAAAGAAGGAGTGT+TGG | 0.556019 | 7:-14812515 | MsG0780036837.01.T01:CDS |
| AAGGCTCTACACTCTTCGAA+TGG | 0.561615 | 7:-14812925 | MsG0780036837.01.T01:CDS |
| AAGAAGAATATCAAATGCTA+CGG | 0.565526 | 7:-14812552 | MsG0780036837.01.T01:CDS |
| ACAGAATATTCTTAAAGAGA+AGG | 0.567304 | 7:-14812944 | MsG0780036837.01.T01:CDS |
| TGCCAATTTGCACCTTGCAA+TGG | 0.571838 | 7:-14813060 | MsG0780036837.01.T01:CDS |
| CCCAGTGGGAGTCAAAATCA+TGG | 0.579929 | 7:-14812594 | MsG0780036837.01.T01:CDS |
| GGCTTCTGATTTCAGAATAG+CGG | 0.582400 | 7:-14812841 | MsG0780036837.01.T01:CDS |
| GATGTGATCCGTTACTGATG+TGG | 0.590297 | 7:+14812896 | None:intergenic |
| TTGCAAGTACCTCAGATGAC+GGG | 0.592205 | 7:-14812437 | MsG0780036837.01.T01:CDS |
| CCTTATCTTCAATTTCCACA+GGG | 0.599059 | 7:+14813178 | None:intergenic |
| TTTGAGATAGAGAAGTTCAA+CGG | 0.599224 | 7:-14813212 | MsG0780036837.01.T01:CDS |
| GGGTGAATCCACATCAGTAA+CGG | 0.599950 | 7:-14812904 | MsG0780036837.01.T01:CDS |
| CAACAGAACGTGGCCCCAGT+GGG | 0.600348 | 7:-14812608 | MsG0780036837.01.T01:CDS |
| TGCAAGGTGCAAATTGGCAA+CGG | 0.612113 | 7:+14813064 | None:intergenic |
| GCTACGGTTGTGGCATGAGA+GGG | 0.613787 | 7:-14812536 | MsG0780036837.01.T01:CDS |
| ATACCGAGTCTGCCATTGCA+AGG | 0.620749 | 7:+14813048 | None:intergenic |
| GATGTCTGGATCATGGACTC+AGG | 0.621640 | 7:-14812361 | MsG0780036837.01.T01:CDS |
| GTTGCAAGTACCTCAGATGA+CGG | 0.628339 | 7:-14812438 | MsG0780036837.01.T01:CDS |
| GTGGGAGTCAAAATCATGGT+AGG | 0.629203 | 7:-14812590 | MsG0780036837.01.T01:CDS |
| ACAGGATTTCCCGTCATCTG+AGG | 0.634137 | 7:+14812428 | None:intergenic |
| AGAGAGTTCAAAGCAAGCAG+AGG | 0.634672 | 7:-14812655 | MsG0780036837.01.T01:CDS |
| TGCTGAAAAGAAGACAGCAA+AGG | 0.637253 | 7:-14813015 | MsG0780036837.01.T01:CDS |
| TCAACAGAACGTGGCCCCAG+TGG | 0.643235 | 7:-14812609 | MsG0780036837.01.T01:CDS |
| TCATGGACTCAGGTGCAACA+TGG | 0.644772 | 7:-14812351 | MsG0780036837.01.T01:CDS |
| GAGGCTTTGATGATGACGAG+AGG | 0.676145 | 7:-14812636 | MsG0780036837.01.T01:CDS |
| TACTCTAATAAAATTGTACG+AGG | 0.683770 | 7:-14812982 | MsG0780036837.01.T01:CDS |
| GCAGCAACCAGTTCTAAAGG+CGG | 0.699528 | 7:-14812397 | MsG0780036837.01.T01:CDS |
| AGAGGCAGATCAACAGAACG+TGG | 0.720628 | 7:-14812618 | MsG0780036837.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAGAATATCAAATGCTA+CGG | - | Chr7:14813016-14813035 | MsG0780036837.01.T01:CDS | 25.0% |
| ! | ACAGAATATTCTTAAAGAGA+AGG | - | Chr7:14812624-14812643 | MsG0780036837.01.T01:CDS | 25.0% |
| ! | TACTCTAATAAAATTGTACG+AGG | - | Chr7:14812586-14812605 | MsG0780036837.01.T01:CDS | 25.0% |
| !! | TCTTTAAGAATATTCTGTTG+TGG | + | Chr7:14812622-14812641 | None:intergenic | 25.0% |
| TGTTGGAATATCAAGAAGAA+TGG | - | Chr7:14813070-14813089 | MsG0780036837.01.T01:CDS | 30.0% | |
| TTGAGAAAATAGCGTATTCA+AGG | + | Chr7:14812702-14812721 | None:intergenic | 30.0% | |
| ! | TTTGAGATAGAGAAGTTCAA+CGG | - | Chr7:14812356-14812375 | MsG0780036837.01.T01:CDS | 30.0% |
| AATATCAAATGCTACGGTTG+TGG | - | Chr7:14813022-14813041 | MsG0780036837.01.T01:CDS | 35.0% | |
| CCTGTGGAAATTGAAGATAA+GGG | - | Chr7:14812391-14812410 | MsG0780036837.01.T01:CDS | 35.0% | |
| CCTTATCTTCAATTTCCACA+GGG | + | Chr7:14812393-14812412 | None:intergenic | 35.0% | |
| TGACGAAAAGTGGAAAGAAA+TGG | - | Chr7:14812472-14812491 | MsG0780036837.01.T01:CDS | 35.0% | |
| TTGAGATAGAGAAGTTCAAC+GGG | - | Chr7:14812357-14812376 | MsG0780036837.01.T01:CDS | 35.0% | |
| !! | ATGAGTTGATCATACGAATC+TGG | + | Chr7:14812788-14812807 | None:intergenic | 35.0% |
| !! | TACGCTATTTTCTCAACTCA+CGG | - | Chr7:14812706-14812725 | MsG0780036837.01.T01:CDS | 35.0% |
| AGAAGACAGCAAAGGAGATT+TGG | - | Chr7:14812561-14812580 | MsG0780036837.01.T01:CDS | 40.0% | |
| CAATCCTTGAAGAAGAATCC+AGG | - | Chr7:14812867-14812886 | MsG0780036837.01.T01:CDS | 40.0% | |
| CAGATATCACTGACGAAAAG+TGG | - | Chr7:14812462-14812481 | MsG0780036837.01.T01:CDS | 40.0% | |
| CCCTGTGGAAATTGAAGATA+AGG | - | Chr7:14812390-14812409 | MsG0780036837.01.T01:CDS | 40.0% | |
| CCCTTATCTTCAATTTCCAC+AGG | + | Chr7:14812394-14812413 | None:intergenic | 40.0% | |
| GAAGACAGCAAAGGAGATTT+GGG | - | Chr7:14812562-14812581 | MsG0780036837.01.T01:CDS | 40.0% | |
| GGCTTCTGATTTCAGAATAG+CGG | - | Chr7:14812727-14812746 | MsG0780036837.01.T01:CDS | 40.0% | |
| GTCTGTCATCTATTGCGTTT+AGG | + | Chr7:14812439-14812458 | None:intergenic | 40.0% | |
| TGCTGAAAAGAAGACAGCAA+AGG | - | Chr7:14812553-14812572 | MsG0780036837.01.T01:CDS | 40.0% | |
| TTCTTCTTCAAGGATTGCAC+CGG | + | Chr7:14812864-14812883 | None:intergenic | 40.0% | |
| ! | TCTGAAGCTTCAACATCTCA+AGG | - | Chr7:14813103-14813122 | MsG0780036837.01.T01:CDS | 40.0% |
| ! | TTTTCGTCAGTGATATCTGC+AGG | + | Chr7:14812461-14812480 | None:intergenic | 40.0% |
| AAGGCTCTACACTCTTCGAA+TGG | - | Chr7:14812643-14812662 | MsG0780036837.01.T01:CDS | 45.0% | |
| ACTCAACGATGTCTGGATCA+TGG | - | Chr7:14813200-14813219 | MsG0780036837.01.T01:CDS | 45.0% | |
| AGAGAGTTCAAAGCAAGCAG+AGG | - | Chr7:14812913-14812932 | MsG0780036837.01.T01:CDS | 45.0% | |
| AGGCTCTACACTCTTCGAAT+GGG | - | Chr7:14812644-14812663 | MsG0780036837.01.T01:CDS | 45.0% | |
| GATGTGATCCGTTACTGATG+TGG | + | Chr7:14812675-14812694 | None:intergenic | 45.0% | |
| GGGTGAATCCACATCAGTAA+CGG | - | Chr7:14812664-14812683 | MsG0780036837.01.T01:CDS | 45.0% | |
| GTGGGAGTCAAAATCATGGT+AGG | - | Chr7:14812978-14812997 | MsG0780036837.01.T01:CDS | 45.0% | |
| GTTGCAAGTACCTCAGATGA+CGG | - | Chr7:14813130-14813149 | MsG0780036837.01.T01:CDS | 45.0% | |
| TGCAAGGTGCAAATTGGCAA+CGG | + | Chr7:14812507-14812526 | None:intergenic | 45.0% | |
| TGCCAATTTGCACCTTGCAA+TGG | - | Chr7:14812508-14812527 | MsG0780036837.01.T01:CDS | 45.0% | |
| TGCCATTGCAAGGTGCAAAT+TGG | + | Chr7:14812513-14812532 | None:intergenic | 45.0% | |
| TTGCAAGTACCTCAGATGAC+GGG | - | Chr7:14813131-14813150 | MsG0780036837.01.T01:CDS | 45.0% | |
| ! | ACGGGAGAAATTTTTCCCTG+TGG | - | Chr7:14812375-14812394 | MsG0780036837.01.T01:CDS | 45.0% |
| ! | GAAGCAGCAACCAGTTCTAA+AGG | - | Chr7:14813168-14813187 | MsG0780036837.01.T01:CDS | 45.0% |
| !! | CTTCACTTTGATGATGTCGC+CGG | - | Chr7:14812842-14812861 | MsG0780036837.01.T01:CDS | 45.0% |
| !!! | ACCATGATTTTGACTCCCAC+TGG | + | Chr7:14812978-14812997 | None:intergenic | 45.0% |
| !!! | CATGATTTTGACTCCCACTG+GGG | + | Chr7:14812976-14812995 | None:intergenic | 45.0% |
| !!! | CCATGATTTTGACTCCCACT+GGG | + | Chr7:14812977-14812996 | None:intergenic | 45.0% |
| ACAGGATTTCCCGTCATCTG+AGG | + | Chr7:14813143-14813162 | None:intergenic | 50.0% | |
| AGAATCCAGGCGCAAGAACA+AGG | - | Chr7:14812880-14812899 | MsG0780036837.01.T01:CDS | 50.0% | |
| AGAGGCAGATCAACAGAACG+TGG | - | Chr7:14812950-14812969 | MsG0780036837.01.T01:CDS | 50.0% | |
| ATACCGAGTCTGCCATTGCA+AGG | + | Chr7:14812523-14812542 | None:intergenic | 50.0% | |
| CCCAGTGGGAGTCAAAATCA+TGG | - | Chr7:14812974-14812993 | MsG0780036837.01.T01:CDS | 50.0% | |
| GAAGACGACTCAACGATGTC+TGG | - | Chr7:14813193-14813212 | MsG0780036837.01.T01:CDS | 50.0% | |
| GATGTCTGGATCATGGACTC+AGG | - | Chr7:14813207-14813226 | MsG0780036837.01.T01:CDS | 50.0% | |
| GGCGCAAGAACAAGGAAGAA+AGG | - | Chr7:14812888-14812907 | MsG0780036837.01.T01:CDS | 50.0% | |
| TCATGGACTCAGGTGCAACA+TGG | - | Chr7:14813217-14813236 | MsG0780036837.01.T01:CDS | 50.0% | |
| TGCGCCTGGATTCTTCTTCA+AGG | + | Chr7:14812874-14812893 | None:intergenic | 50.0% | |
| TTCTTCCTTGTTCTTGCGCC+TGG | + | Chr7:14812888-14812907 | None:intergenic | 50.0% | |
| ! | CATGAGAGGGCACGTAAAGA+AGG | - | Chr7:14813045-14813064 | MsG0780036837.01.T01:CDS | 50.0% |
| ! | GGCACGTAAAGAAGGAGTGT+TGG | - | Chr7:14813053-14813072 | MsG0780036837.01.T01:CDS | 50.0% |
| ! | TGGTTGCTGCTTCGCTATAC+AGG | + | Chr7:14813161-14813180 | None:intergenic | 50.0% |
| !! | GAGGCTTTGATGATGACGAG+AGG | - | Chr7:14812932-14812951 | MsG0780036837.01.T01:CDS | 50.0% |
| !! | GCAGCAACCAGTTCTAAAGG+CGG | - | Chr7:14813171-14813190 | MsG0780036837.01.T01:CDS | 50.0% |
| !! | TCGTCTTCCGCCTTTAGAAC+TGG | + | Chr7:14813181-14813200 | None:intergenic | 50.0% |
| GCACCTTGCAATGGCAGACT+CGG | - | Chr7:14812517-14812536 | MsG0780036837.01.T01:CDS | 55.0% | |
| GCTACGGTTGTGGCATGAGA+GGG | - | Chr7:14813032-14813051 | MsG0780036837.01.T01:CDS | 55.0% | |
| TGCTACGGTTGTGGCATGAG+AGG | - | Chr7:14813031-14813050 | MsG0780036837.01.T01:CDS | 55.0% | |
| CAACAGAACGTGGCCCCAGT+GGG | - | Chr7:14812960-14812979 | MsG0780036837.01.T01:CDS | 60.0% | |
| TCAACAGAACGTGGCCCCAG+TGG | - | Chr7:14812959-14812978 | MsG0780036837.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 14812344 | 14813246 | 14812344 | ID=MsG0780036837.01;Name=MsG0780036837.01 |
| Chr7 | mRNA | 14812344 | 14813246 | 14812344 | ID=MsG0780036837.01.T01;Parent=MsG0780036837.01;Name=MsG0780036837.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|300 |
| Chr7 | exon | 14812344 | 14813246 | 14812344 | ID=MsG0780036837.01.T01:exon:2987;Parent=MsG0780036837.01.T01 |
| Chr7 | CDS | 14812344 | 14813246 | 14812344 | ID=MsG0780036837.01.T01:cds;Parent=MsG0780036837.01.T01 |
| Gene Sequence |
| Protein sequence |
