AlfalfaGEDB | MsG0180002185.01

M. sativa cultivar ZhongmuNo.1 / MsG0180002185.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002185.01.T01	KOM55676.1	91.139	79	7	0	1	79	1	79	5.67E-43	145

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002185.01.T01	A0A0L9VKT4	91.139	79	7	0	1	79	1	79	2.71e-43	145

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000680.01	MsG0180002185.01	0.825751	3.950857e-54	3.633529e-51
MsG0180000741.01	MsG0180002185.01	0.826030	3.391691e-54	3.144093e-51
MsG0180000797.01	MsG0180002185.01	0.819095	1.390497e-52	1.059889e-49
MsG0180000855.01	MsG0180002185.01	0.807808	4.217106e-50	2.371618e-47
MsG0180001922.01	MsG0180002185.01	0.814859	1.242806e-51	8.434251e-49
MsG0180002185.01	MsG0180002300.01	0.807171	5.759342e-50	3.185620e-47
MsG0180002185.01	MsG0180002796.01	0.802393	5.731110e-49	2.804288e-46
MsG0180002185.01	MsG0180003303.01	0.807832	4.169826e-50	2.346409e-47
MsG0180002185.01	MsG0180003807.01	0.816226	6.166733e-52	4.344534e-49
MsG0180002185.01	MsG0180003977.01	0.804394	2.206068e-49	1.136144e-46
MsG0180002185.01	MsG0180004437.01	0.825104	5.623688e-54	5.077471e-51
MsG0180002185.01	MsG0180005106.01	0.804713	1.893249e-49	9.831302e-47
MsG0180002185.01	MsG0180005354.01	0.812823	3.492313e-51	2.242913e-48
MsG0180002185.01	MsG0180005786.01	0.807521	4.853130e-50	2.709027e-47
MsG0180002185.01	MsG0180005944.01	0.813767	2.166277e-51	1.427303e-48
MsG0180002185.01	MsG0180006050.01	0.846263	2.424250e-59	4.148806e-56
MsG0180002185.01	MsG0280006367.01	0.825361	4.887119e-54	4.444697e-51
MsG0180002185.01	MsG0280006440.01	0.803476	3.424040e-49	1.722319e-46
MsG0180002185.01	MsG0280006892.01	0.801543	8.566686e-49	4.102000e-46
MsG0180002185.01	MsG0280006935.01	0.810516	1.108762e-50	6.697246e-48
MsG0180002185.01	MsG0280007011.01	0.808207	3.469280e-50	1.971730e-47
MsG0180002185.01	MsG0280007109.01	0.809001	2.347859e-50	1.362435e-47
MsG0180002185.01	MsG0280007585.01	0.814366	1.598010e-51	1.070170e-48
MsG0180002185.01	MsG0280007691.01	0.816390	5.667442e-52	4.010604e-49
MsG0180002185.01	MsG0280007775.01	0.831557	1.560136e-55	1.699148e-52
MsG0180002185.01	MsG0280008234.01	0.812434	4.247871e-51	2.700780e-48
MsG0180002185.01	MsG0280008720.01	0.805732	1.158157e-49	6.174236e-47
MsG0180002185.01	MsG0280009270.01	0.810144	1.334033e-50	7.979405e-48
MsG0180002185.01	MsG0280009350.01	0.803569	3.274577e-49	1.651224e-46
MsG0180002185.01	MsG0280009554.01	0.807838	4.156707e-50	2.339433e-47
MsG0180002185.01	MsG0280010816.01	0.809505	1.830694e-50	1.076758e-47
MsG0180002185.01	MsG0280010976.01	0.828389	9.236967e-55	9.168462e-52
MsG0180002185.01	MsG0280011043.01	0.822241	2.632216e-53	2.190780e-50
MsG0180002185.01	MsG0380013471.01	0.810581	1.073738e-50	6.496969e-48
MsG0180002185.01	MsG0380014880.01	0.820348	7.192984e-53	5.677273e-50
MsG0180002185.01	MsG0380015614.01	0.827595	1.434241e-54	1.390811e-51
MsG0180002185.01	MsG0380016223.01	0.830380	3.034837e-55	3.192045e-52
MsG0180002185.01	MsG0380017210.01	0.821864	3.218206e-53	2.649992e-50
MsG0180002185.01	MsG0380017229.01	0.823208	1.567654e-53	1.341036e-50
MsG0180002185.01	MsG0380017528.01	0.806146	9.477469e-50	5.105982e-47
MsG0180002185.01	MsG0380017719.01	0.801704	7.942759e-49	3.818607e-46
MsG0180002185.01	MsG0380017914.01	-0.805451	1.326533e-49	7.020462e-47
MsG0180002185.01	MsG0480019289.01	0.825251	5.189974e-54	4.705531e-51
MsG0180002185.01	MsG0480019401.01	0.805124	1.552799e-49	8.149417e-47
MsG0180002185.01	MsG0480019785.01	0.806912	6.533189e-50	3.589465e-47
MsG0180002185.01	MsG0480020540.01	0.830031	3.691194e-55	3.843382e-52
MsG0180002185.01	MsG0480020719.01	0.810464	1.137894e-50	6.863720e-48
MsG0180002185.01	MsG0480021204.01	0.800223	1.594233e-48	7.382739e-46
MsG0180002185.01	MsG0480021240.01	0.804005	2.658029e-49	1.355373e-46
MsG0180002185.01	MsG0480022169.01	0.809048	2.293320e-50	1.332530e-47
MsG0180002185.01	MsG0480022203.01	0.807265	5.499511e-50	3.049464e-47
MsG0180002185.01	MsG0480022552.01	0.808877	2.495331e-50	1.443227e-47
MsG0180002185.01	MsG0480022678.01	0.804830	1.788918e-49	9.318101e-47
MsG0180002185.01	MsG0480023554.01	0.812435	4.244977e-51	2.699053e-48
MsG0180002185.01	MsG0480023659.01	0.806779	6.970779e-50	3.816680e-47
MsG0180002185.01	MsG0480023729.01	0.816977	4.186672e-52	3.010931e-49
MsG0180002185.01	MsG0580024581.01	0.816569	5.169123e-52	3.675865e-49
MsG0180002185.01	MsG0580025922.01	0.811484	6.841422e-51	4.240135e-48
MsG0180002185.01	MsG0580029629.01	0.804917	1.715815e-49	8.957637e-47
MsG0180002185.01	MsG0580029639.01	0.813496	2.485115e-51	1.625545e-48
MsG0180002185.01	MsG0580029658.01	0.805519	1.283842e-49	6.806087e-47
MsG0180002185.01	MsG0680031301.01	0.823343	1.457648e-53	1.251638e-50
MsG0180002185.01	MsG0680034476.01	0.817604	3.024929e-52	2.212943e-49
MsG0180002185.01	MsG0680034757.01	0.803058	4.177688e-49	2.078933e-46
MsG0180002185.01	MsG0780036633.01	0.822514	2.274602e-53	1.908073e-50
MsG0180002185.01	MsG0780036837.01	0.800415	1.456547e-48	6.778423e-46
MsG0180002185.01	MsG0780036845.01	0.811883	5.603439e-51	3.509499e-48
MsG0180002185.01	MsG0780038092.01	0.821802	3.326887e-53	2.734562e-50
MsG0180002185.01	MsG0780039587.01	0.806802	6.892525e-50	3.776112e-47
MsG0180002185.01	MsG0780039777.01	0.808231	3.428569e-50	1.949777e-47
MsG0180002185.01	MsG0780040125.01	0.817077	3.975074e-52	2.866360e-49
MsG0180002185.01	MsG0780040258.01	0.806818	6.839037e-50	3.748476e-47
MsG0180002185.01	MsG0780040712.01	0.805069	1.594918e-49	8.358536e-47
MsG0180002185.01	MsG0780040742.01	0.815614	8.447074e-52	5.852552e-49
MsG0180002185.01	MsG0780041210.01	0.822123	2.803250e-53	2.325262e-50
MsG0180002185.01	MsG0780041498.01	0.806753	7.058045e-50	3.861944e-47
MsG0180002185.01	MsG0880043162.01	0.836925	7.031063e-57	8.991249e-54
MsG0180002185.01	MsG0880043341.01	0.829834	4.124686e-55	4.271229e-52
MsG0180002185.01	MsG0880043351.01	0.804244	2.371074e-49	1.216367e-46
MsG0180002185.01	MsG0880045869.01	0.829962	3.837210e-55	3.987637e-52
MsG0180002185.01	MsG0880047745.01	0.800212	1.602539e-48	7.419106e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002185.01.T01	MTR_7g069120	80.303	66	13	0	1	66	1	66	3.77e-31	114

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 16 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGACAGCAAAGGAGATTT+AGG	0.263020	1:-34425079	None:intergenic
TGCCATTGCAAGGTGCAAAT+TGG	0.377007	1:+34425131	None:intergenic
CCCTGTGGAAATTGAAGATA+AGG	0.385271	1:-34425251	MsG0180002185.01.T01:CDS
CCCTTATCTTCAATTTCCAC+AGG	0.443822	1:+34425250	None:intergenic
TGACGAAAAGTGGAAAGAAA+TGG	0.447767	1:-34425169	MsG0180002185.01.T01:CDS
TTGAGATAGAGAAGTTCAAC+GGG	0.451269	1:-34425284	MsG0180002185.01.T01:CDS
CAGATATCACTGACGAAAAG+TGG	0.458224	1:-34425179	MsG0180002185.01.T01:CDS
GTCTGTCATCTATTGCGTTT+AGG	0.458864	1:+34425205	None:intergenic
CCTGTGGAAATTGAAGATAA+GGG	0.535816	1:-34425250	MsG0180002185.01.T01:CDS
GCACCTTGCAATGGCAGACT+CGG	0.544274	1:-34425124	MsG0180002185.01.T01:CDS
TGCCAATTTGCACCTTGCAA+TGG	0.571838	1:-34425133	MsG0180002185.01.T01:CDS
CCTTATCTTCAATTTCCACA+GGG	0.599059	1:+34425251	None:intergenic
TTTGAGATAGAGAAGTTCAA+CGG	0.599224	1:-34425285	MsG0180002185.01.T01:CDS
TGCAAGGTGCAAATTGGCAA+CGG	0.612113	1:+34425137	None:intergenic
ATACCGAGTCTGCCATTGCA+AGG	0.620749	1:+34425121	None:intergenic
TGCTGAAAAGAAGACAGCAA+AGG	0.637253	1:-34425088	MsG0180002185.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTGAGATAGAGAAGTTCAA+CGG	-	Chr1:34425092-34425111	MsG0180002185.01.T01:CDS	30.0%
	CCTGTGGAAATTGAAGATAA+GGG	-	Chr1:34425127-34425146	MsG0180002185.01.T01:CDS	35.0%
	CCTTATCTTCAATTTCCACA+GGG	+	Chr1:34425129-34425148	None:intergenic	35.0%
	TGACGAAAAGTGGAAAGAAA+TGG	-	Chr1:34425208-34425227	MsG0180002185.01.T01:CDS	35.0%
	TTGAGATAGAGAAGTTCAAC+GGG	-	Chr1:34425093-34425112	MsG0180002185.01.T01:CDS	35.0%
	CAGATATCACTGACGAAAAG+TGG	-	Chr1:34425198-34425217	MsG0180002185.01.T01:CDS	40.0%
	CCCTGTGGAAATTGAAGATA+AGG	-	Chr1:34425126-34425145	MsG0180002185.01.T01:CDS	40.0%
	CCCTTATCTTCAATTTCCAC+AGG	+	Chr1:34425130-34425149	None:intergenic	40.0%
	GTCTGTCATCTATTGCGTTT+AGG	+	Chr1:34425175-34425194	None:intergenic	40.0%
	TGCTGAAAAGAAGACAGCAA+AGG	-	Chr1:34425289-34425308	MsG0180002185.01.T01:CDS	40.0%
!	TTTTCGTCAGTGATATCTGC+AGG	+	Chr1:34425197-34425216	None:intergenic	40.0%
	TGCAAGGTGCAAATTGGCAA+CGG	+	Chr1:34425243-34425262	None:intergenic	45.0%
	TGCCAATTTGCACCTTGCAA+TGG	-	Chr1:34425244-34425263	MsG0180002185.01.T01:CDS	45.0%
	TGCCATTGCAAGGTGCAAAT+TGG	+	Chr1:34425249-34425268	None:intergenic	45.0%
!	ACGGGAGAAATTTTTCCCTG+TGG	-	Chr1:34425111-34425130	MsG0180002185.01.T01:CDS	45.0%
	ATACCGAGTCTGCCATTGCA+AGG	+	Chr1:34425259-34425278	None:intergenic	50.0%
	GCACCTTGCAATGGCAGACT+CGG	-	Chr1:34425253-34425272	MsG0180002185.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	34425080	34425319	34425080	ID=MsG0180002185.01;Name=MsG0180002185.01
Chr1	mRNA	34425080	34425319	34425080	ID=MsG0180002185.01.T01;Parent=MsG0180002185.01;Name=MsG0180002185.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|79
Chr1	exon	34425080	34425319	34425080	ID=MsG0180002185.01.T01:exon:36181;Parent=MsG0180002185.01.T01
Chr1	CDS	34425080	34425319	34425080	ID=MsG0180002185.01.T01:cds;Parent=MsG0180002185.01.T01

Gene Sequence	>MsG0180002185.01.T01 ATGGCAGCAAAATTTGAGATAGAGAAGTTCAACGGGAGAAATTTTTCCCTGTGGAAATTGAAGATAAGGGCAATTTTAAGAAAAGATAATTGCCTAAACGCAATAGATGACAGACCTGCAGATATCACTGACGAAAAGTGGAAAGAAATGGATGATAATGCCGTTGCCAATTTGCACCTTGCAATGGCAGACTCGGTATTGTCAAGTATTGCTGAAAAGAAGACAGCAAAGGAGATTTAG
Protein sequence	>MsG0180002185.01.T01 MAAKFEIEKFNGRNFSLWKLKIRAILRKDNCLNAIDDRPADITDEKWKEMDDNAVANLHLAMADSVLSSIAEKKTAKEI*