AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036912.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036912.01.T01 MTR_6g011470 83.333 66 11 0 105 170 145 210 1.21e-34 121
MsG0780036912.01.T01 MTR_6g011450 82.540 63 11 0 108 170 613 675 9.78e-31 117
MsG0780036912.01.T01 MTR_6g011450 82.540 63 11 0 108 170 751 813 1.04e-30 117
MsG0780036912.01.T01 MTR_8g465040 68.000 75 24 0 2 76 97 171 1.21e-29 107
MsG0780036912.01.T01 MTR_3g452170 54.639 97 44 0 2 98 97 193 3.88e-27 105
MsG0780036912.01.T01 MTR_1g105785 50.000 96 48 0 2 97 97 192 7.62e-27 105
MsG0780036912.01.T01 MTR_7g110100 55.670 97 43 0 2 98 97 193 1.31e-26 105
MsG0780036912.01.T01 MTR_4g073340 75.806 62 15 0 109 170 433 494 4.59e-26 103
MsG0780036912.01.T01 MTR_1g051235 60.526 76 29 1 2 76 97 172 4.06e-24 92.8
MsG0780036912.01.T01 MTR_7g051840 54.667 75 31 1 2 76 54 125 1.50e-21 85.1
MsG0780036912.01.T01 MTR_5g019960 42.105 114 57 2 2 106 103 216 5.85e-19 81.3
MsG0780036912.01.T01 MTR_6g465570 48.000 75 39 0 2 76 93 167 1.74e-18 78.2
MsG0780036912.01.T01 MTR_7g045450 47.619 84 44 0 3 86 98 181 2.44e-18 79.7
MsG0780036912.01.T01 MTR_6g073570 47.619 84 44 0 3 86 98 181 2.44e-18 79.7
MsG0780036912.01.T01 MTR_8g005940 45.238 84 46 0 3 86 98 181 1.59e-16 75.5
MsG0780036912.01.T01 MTR_3g007260 48.780 82 42 0 15 96 70 151 2.94e-16 72.4
MsG0780036912.01.T01 MTR_7g451880 42.857 84 48 0 3 86 99 182 9.56e-16 73.6
MsG0780036912.01.T01 MTR_7g051400 46.875 96 46 1 2 97 97 187 1.32e-15 73.9
MsG0780036912.01.T01 MTR_3g015660 42.857 84 48 0 3 86 98 181 2.54e-15 71.2
MsG0780036912.01.T01 MTR_1g052715 39.583 96 57 1 2 96 56 151 3.40e-15 72.0
MsG0780036912.01.T01 MTR_7g074180 41.667 84 49 0 3 86 98 181 1.38e-14 70.5
MsG0780036912.01.T01 MTR_4g058660 41.667 84 49 0 3 86 98 181 2.26e-14 69.7
MsG0780036912.01.T01 MTR_0105s0080 40.476 84 50 0 3 86 54 137 6.51e-14 67.8
MsG0780036912.01.T01 MTR_5g045850 40.476 84 50 0 3 86 83 166 9.76e-14 67.8
MsG0780036912.01.T01 MTR_1g023370 41.667 84 49 0 3 86 98 181 1.02e-13 67.8
MsG0780036912.01.T01 MTR_7g024060 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_6g087820 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_5g081190 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_5g091480 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_5g056290 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_3g031190 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_2g005490 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_2g010570 41.667 84 49 0 3 86 98 181 1.03e-13 67.8
MsG0780036912.01.T01 MTR_5g030110 41.667 84 49 0 3 86 98 181 1.10e-13 67.8
MsG0780036912.01.T01 MTR_2g007410 41.667 84 49 0 3 86 98 181 1.10e-13 67.8
MsG0780036912.01.T01 MTR_8g446640 41.667 84 49 0 3 86 79 162 1.48e-13 67.4
MsG0780036912.01.T01 MTR_2g010270 37.634 93 49 1 3 86 79 171 3.46e-12 63.5
MsG0780036912.01.T01 MTR_4g017450 65.000 40 14 0 2 41 96 135 2.71e-11 58.5
MsG0780036912.01.T01 MTR_7g103840 47.541 61 31 1 110 170 954 1013 3.30e-11 61.2
MsG0780036912.01.T01 MTR_7g103840 47.541 61 31 1 110 170 956 1015 3.50e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036912.01.T01 AT4G04885 70.769 65 19 0 105 169 725 789 1.18e-28 111

Find 33 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAACACTCTCATCAATTT+TGG 0.201853 7:+16177106 None:intergenic
TTATTTCATTCGGCGCTTTA+AGG 0.260900 7:+16175433 None:intergenic
CTGCATTTAGCATGAATAAT+GGG 0.336904 7:+16175381 None:intergenic
TCCATGAAGATGTACCTTGC+AGG 0.355379 7:+16176032 None:intergenic
TCTGCATTTAGCATGAATAA+TGG 0.356112 7:+16175380 None:intergenic
ATGGAAATTCAGAAGATTGA+TGG 0.364816 7:-16176014 MsG0780036912.01.T01:CDS
ATGCCTGTTGTTATTTCATT+CGG 0.370130 7:+16175423 None:intergenic
TTTACCTTTGCAGATGTCAT+TGG 0.390875 7:-16176940 MsG0780036912.01.T01:intron
TTAACGGAGAAAGATGTTAA+TGG 0.395880 7:-16176895 MsG0780036912.01.T01:CDS
GCCTGCAAGGTACATCTTCA+TGG 0.400385 7:-16176033 MsG0780036912.01.T01:CDS
ACAAAGTCGAAATGGTTAAC+AGG 0.403199 7:+16177083 None:intergenic
TAAAAGAAACAAAGTCGAAA+TGG 0.435702 7:+16177075 None:intergenic
TATTGAGAAGGATGTGTTAA+CGG 0.441579 7:-16176911 MsG0780036912.01.T01:CDS
GGCATGGACAGGTCGCTGTT+AGG 0.459993 7:-16175405 MsG0780036912.01.T01:CDS
ACAGTGATGAAACGGAGAAC+TGG 0.463316 7:-16175476 MsG0780036912.01.T01:CDS
GCGCCGAATGAAATAACAAC+AGG 0.522849 7:-16175426 MsG0780036912.01.T01:CDS
CGTGTCTTACAGTGATGAAA+CGG 0.534581 7:-16175484 MsG0780036912.01.T01:CDS
AAATAACAACAGGCATGGAC+AGG 0.551052 7:-16175416 MsG0780036912.01.T01:CDS
CAAAATGCATGTGCATTATG+TGG 0.555248 7:-16175519 MsG0780036912.01.T01:CDS
TCACTGTAAGACACGTCAAA+AGG 0.557622 7:+16175492 None:intergenic
TAAGCTAATTGACATAACCT+TGG 0.559600 7:-16176863 MsG0780036912.01.T01:CDS
AAGCTGTACGGATGAAGAGT+TGG 0.560481 7:-16175562 MsG0780036912.01.T01:CDS
CATTGGTCATATTATTGAGA+AGG 0.561384 7:-16176923 MsG0780036912.01.T01:CDS
AGGTTCGTCATGAAGCTGTA+CGG 0.566922 7:-16175574 MsG0780036912.01.T01:intron
TACTTACTCAGTATCTTCCA+AGG 0.582186 7:+16176846 None:intergenic
AACAGAGAGGATAAGCATGT+TGG 0.592206 7:-16177035 MsG0780036912.01.T01:intron
GAATGAAATAACAACAGGCA+TGG 0.593565 7:-16175421 MsG0780036912.01.T01:CDS
CTTATTTCTCAAAGCCTGCA+AGG 0.597090 7:-16176046 MsG0780036912.01.T01:CDS
TGCATTTAGCATGAATAATG+GGG 0.635399 7:+16175382 None:intergenic
ACGGAGAACTGGATGTGCAG+AGG 0.647467 7:-16175465 MsG0780036912.01.T01:CDS
TGATGGCAATTATTATCCTG+AGG 0.655513 7:-16175997 MsG0780036912.01.T01:intron
TATATTACAATCTAACAGAG+AGG 0.701782 7:-16177048 MsG0780036912.01.T01:CDS
GTTGGCAGTTCCGTCCGACG+AGG 0.708375 7:-16175544 MsG0780036912.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAGAGAAAATAATA+AGG + Chr7:16175928-16175947 None:intergenic 10.0%
!! ATTTATTATATGGTCTAATA+AGG - Chr7:16176407-16176426 MsG0780036912.01.T01:intron 15.0%
!! GTGTTTCATAATTTATTATA+TGG - Chr7:16176397-16176416 MsG0780036912.01.T01:intron 15.0%
!!! AATTTTAATCAACTTCAATA+GGG + Chr7:16176721-16176740 None:intergenic 15.0%
!!! GTGTTTTAATTTCATATTTT+TGG - Chr7:16175529-16175548 MsG0780036912.01.T01:CDS 15.0%
!! ACAGAATACAATAACAAAAT+CGG + Chr7:16176880-16176899 None:intergenic 20.0%
!! ATAATTATTGAAGTTGTTGA+GGG - Chr7:16176322-16176341 MsG0780036912.01.T01:intron 20.0%
!!! GAATTTTAATCAACTTCAAT+AGG + Chr7:16176722-16176741 None:intergenic 20.0%
!!! GTAATTTTTTCTCTCTTTTT+TGG - Chr7:16175846-16175865 MsG0780036912.01.T01:intron 20.0%
! AAAATTGAAAGTTGAAGTTG+CGG + Chr7:16175821-16175840 None:intergenic 25.0%
! AAACTCTTCATTATTAATGC+TGG - Chr7:16176767-16176786 MsG0780036912.01.T01:intron 25.0%
! GATAATTATTGAAGTTGTTG+AGG - Chr7:16176321-16176340 MsG0780036912.01.T01:intron 25.0%
! TAAAAGAAACAAAGTCGAAA+TGG + Chr7:16175445-16175464 None:intergenic 25.0%
! TAATTATTGAAGTTGTTGAG+GGG - Chr7:16176323-16176342 MsG0780036912.01.T01:intron 25.0%
! TATAGATGATTCTCATTTAC+CGG + Chr7:16176021-16176040 None:intergenic 25.0%
! TATATTACAATCTAACAGAG+AGG - Chr7:16175469-16175488 MsG0780036912.01.T01:CDS 25.0%
! TGTTATTGTATTCTGTTTGT+AGG - Chr7:16176884-16176903 MsG0780036912.01.T01:CDS 25.0%
!! AAACTTTGATTTCTCTGAAT+TGG + Chr7:16176212-16176231 None:intergenic 25.0%
!! AACTTTGATTTCTCTGAATT+GGG + Chr7:16176211-16176230 None:intergenic 25.0%
!! TATAACACTCTCATCAATTT+TGG + Chr7:16175414-16175433 None:intergenic 25.0%
!! TTCTGTTTGTAGGATTTTAT+TGG - Chr7:16176894-16176913 MsG0780036912.01.T01:CDS 25.0%
!!! ATATTGGTATCTCCTTTTTT+TGG + Chr7:16176154-16176173 None:intergenic 25.0%
!!! GAATATGCATGATTTTGATA+TGG - Chr7:16176253-16176272 MsG0780036912.01.T01:intron 25.0%
!!! TAGTCTATTTTTTTCACCAT+GGG + Chr7:16176084-16176103 None:intergenic 25.0%
!!! TTAGTCTATTTTTTTCACCA+TGG + Chr7:16176085-16176104 None:intergenic 25.0%
ATGGAAATTCAGAAGATTGA+TGG - Chr7:16176503-16176522 MsG0780036912.01.T01:intron 30.0%
CATTGGTCATATTATTGAGA+AGG - Chr7:16175594-16175613 MsG0780036912.01.T01:intron 30.0%
CTCTTTCATATGCTTCATAT+TGG + Chr7:16176170-16176189 None:intergenic 30.0%
CTGCATTTAGCATGAATAAT+GGG + Chr7:16177139-16177158 None:intergenic 30.0%
GAATATTTGGATCAAACTCA+AGG + Chr7:16176920-16176939 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG - Chr7:16176923-16176942 MsG0780036912.01.T01:CDS 30.0%
TAAGCTAATTGACATAACCT+TGG - Chr7:16175654-16175673 MsG0780036912.01.T01:intron 30.0%
TATTGAGAAGGATGTGTTAA+CGG - Chr7:16175606-16175625 MsG0780036912.01.T01:intron 30.0%
TCTGCATTTAGCATGAATAA+TGG + Chr7:16177140-16177159 None:intergenic 30.0%
TGCATTTAGCATGAATAATG+GGG + Chr7:16177138-16177157 None:intergenic 30.0%
TTAACGGAGAAAGATGTTAA+TGG - Chr7:16175622-16175641 MsG0780036912.01.T01:intron 30.0%
!! ATGCCTGTTGTTATTTCATT+CGG + Chr7:16177097-16177116 None:intergenic 30.0%
!! TAAAATGATAAGGGTACTTC+TGG - Chr7:16176126-16176145 MsG0780036912.01.T01:intron 30.0%
!! TTCATTATTAATGCTGGTTC+TGG - Chr7:16176773-16176792 MsG0780036912.01.T01:intron 30.0%
ACAAAGTCGAAATGGTTAAC+AGG + Chr7:16175437-16175456 None:intergenic 35.0%
ATGGTCTAATAAGGCTGATT+TGG - Chr7:16176416-16176435 MsG0780036912.01.T01:intron 35.0%
CAAAATGCATGTGCATTATG+TGG - Chr7:16176998-16177017 MsG0780036912.01.T01:intron 35.0%
CAGTTTAGTAGAAGCTGAAT+TGG - Chr7:16175884-16175903 MsG0780036912.01.T01:intron 35.0%
CGTAGATTAGATTTATGTGC+CGG - Chr7:16175999-16176018 MsG0780036912.01.T01:CDS 35.0%
GAATGAAATAACAACAGGCA+TGG - Chr7:16177096-16177115 MsG0780036912.01.T01:CDS 35.0%
GTGGTTGTATTATTCTTCCA+GGG - Chr7:16176443-16176462 MsG0780036912.01.T01:intron 35.0%
TACTTACTCAGTATCTTCCA+AGG + Chr7:16175674-16175693 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG + Chr7:16176933-16176952 None:intergenic 35.0%
TGATGGCAATTATTATCCTG+AGG - Chr7:16176520-16176539 MsG0780036912.01.T01:intron 35.0%
TGTGGTTGTATTATTCTTCC+AGG - Chr7:16176442-16176461 MsG0780036912.01.T01:intron 35.0%
TTATTTCATTCGGCGCTTTA+AGG + Chr7:16177087-16177106 None:intergenic 35.0%
TTGAGAAATAAGACATTCCC+TGG + Chr7:16176463-16176482 None:intergenic 35.0%
TTTACCTTTGCAGATGTCAT+TGG - Chr7:16175577-16175596 MsG0780036912.01.T01:intron 35.0%
!! ATAAGGCTGATTTGGTTATG+TGG - Chr7:16176424-16176443 MsG0780036912.01.T01:intron 35.0%
!! TGATCAACAAAAAGTACCTC+AGG + Chr7:16176539-16176558 None:intergenic 35.0%
AAATAACAACAGGCATGGAC+AGG - Chr7:16177101-16177120 MsG0780036912.01.T01:CDS 40.0%
AACAGAGAGGATAAGCATGT+TGG - Chr7:16175482-16175501 MsG0780036912.01.T01:CDS 40.0%
ACCATGGGTAAAGCTAAACA+GGG + Chr7:16176069-16176088 None:intergenic 40.0%
ACGCAATCAAGCAAAATGGA+AGG + Chr7:16176856-16176875 None:intergenic 40.0%
ATGACCAATGACATCTGCAA+AGG + Chr7:16175584-16175603 None:intergenic 40.0%
CATAGCAGGTCAAACACATA+TGG + Chr7:16175735-16175754 None:intergenic 40.0%
CATATGTGTTTGACCTGCTA+TGG - Chr7:16175733-16175752 MsG0780036912.01.T01:intron 40.0%
CGTGTCTTACAGTGATGAAA+CGG - Chr7:16177033-16177052 MsG0780036912.01.T01:intron 40.0%
GTACTTCTGGCACCAAAAAA+AGG - Chr7:16176139-16176158 MsG0780036912.01.T01:intron 40.0%
TAGCTCATGAGCAGATGATT+TGG - Chr7:16176559-16176578 MsG0780036912.01.T01:intron 40.0%
TCACTGTAAGACACGTCAAA+AGG + Chr7:16177028-16177047 None:intergenic 40.0%
TGAAGAGCAAAATCCATAGC+AGG + Chr7:16175749-16175768 None:intergenic 40.0%
! CTTATTTCTCAAAGCCTGCA+AGG - Chr7:16176471-16176490 MsG0780036912.01.T01:intron 40.0%
!! ACATGCATTTTGATCCTCGT+CGG + Chr7:16176990-16177009 None:intergenic 40.0%
AAGCTGTACGGATGAAGAGT+TGG - Chr7:16176955-16176974 MsG0780036912.01.T01:intron 45.0%
ACAGTGATGAAACGGAGAAC+TGG - Chr7:16177041-16177060 MsG0780036912.01.T01:CDS 45.0%
AGGTTCGTCATGAAGCTGTA+CGG - Chr7:16176943-16176962 MsG0780036912.01.T01:intron 45.0%
CACCATGGGTAAAGCTAAAC+AGG + Chr7:16176070-16176089 None:intergenic 45.0%
CCATGGGTAAAGCTAAACAG+GGG + Chr7:16176068-16176087 None:intergenic 45.0%
CGGTACGCAATCAAGCAAAA+TGG + Chr7:16176860-16176879 None:intergenic 45.0%
GCGCCGAATGAAATAACAAC+AGG - Chr7:16177091-16177110 MsG0780036912.01.T01:CDS 45.0%
TCCATGAAGATGTACCTTGC+AGG + Chr7:16176488-16176507 None:intergenic 45.0%
TTGGTCGTCGAATAATGCAG+CGG - Chr7:16175548-16175567 MsG0780036912.01.T01:CDS 45.0%
!! GTTCTGGCTTTAAGATGTCG+TGG - Chr7:16176789-16176808 MsG0780036912.01.T01:intron 45.0%
!! AAAAATTTATTAAAATGATA+AGG - Chr7:16176116-16176135 MsG0780036912.01.T01:intron 5.0%
!! AAAATTTATTAAAATGATAA+GGG - Chr7:16176117-16176136 MsG0780036912.01.T01:intron 5.0%
AAGTTGAAGTTGCGGCGATG+AGG + Chr7:16175813-16175832 None:intergenic 50.0%
GCCTGCAAGGTACATCTTCA+TGG - Chr7:16176484-16176503 MsG0780036912.01.T01:intron 50.0%
!! CCCCTGTTTAGCTTTACCCA+TGG - Chr7:16176065-16176084 MsG0780036912.01.T01:intron 50.0%
!! GCATTTTGATCCTCGTCGGA+CGG + Chr7:16176986-16177005 None:intergenic 50.0%
ACGGAGAACTGGATGTGCAG+AGG - Chr7:16177052-16177071 MsG0780036912.01.T01:CDS 55.0%
TTGAAGTTGCGGCGATGAGG+TGG + Chr7:16175810-16175829 None:intergenic 55.0%
GGCATGGACAGGTCGCTGTT+AGG - Chr7:16177112-16177131 MsG0780036912.01.T01:CDS 60.0%
GTTGGCAGTTCCGTCCGACG+AGG - Chr7:16176973-16176992 MsG0780036912.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 16175374 16177165 16175374 ID=MsG0780036912.01;Name=MsG0780036912.01
Chr7 mRNA 16175374 16177165 16175374 ID=MsG0780036912.01.T01;Parent=MsG0780036912.01;Name=MsG0780036912.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0.91|0.92|1|0.25|0.23|13|0|170
Chr7 exon 16175374 16175581 16175374 ID=MsG0780036912.01.T01:exon:11312;Parent=MsG0780036912.01.T01
Chr7 exon 16175998 16176075 16175998 ID=MsG0780036912.01.T01:exon:11311;Parent=MsG0780036912.01.T01
Chr7 exon 16176853 16176949 16176853 ID=MsG0780036912.01.T01:exon:11310;Parent=MsG0780036912.01.T01
Chr7 exon 16177036 16177165 16177036 ID=MsG0780036912.01.T01:exon:11309;Parent=MsG0780036912.01.T01
Chr7 CDS 16177036 16177165 16177036 ID=MsG0780036912.01.T01:cds;Parent=MsG0780036912.01.T01
Chr7 CDS 16176853 16176949 16176853 ID=MsG0780036912.01.T01:cds;Parent=MsG0780036912.01.T01
Chr7 CDS 16175998 16176075 16175998 ID=MsG0780036912.01.T01:cds;Parent=MsG0780036912.01.T01
Chr7 CDS 16175374 16175581 16175374 ID=MsG0780036912.01.T01:cds;Parent=MsG0780036912.01.T01
Gene Sequence

>MsG0780036912.01.T01

ATGTTTAAACTCAACTTAATTGATAGAACAAAGTTTACCAAAATTGATGAGAGTGTTATACCTGTTAACCATTTCGACTTTGTTTCTTTTAAAGATATATTACAATCTAACAGAGAGGATAAGCATGTTGATGTCATTGGTCATATTATTGAGAAGGATGTGTTAACGGAGAAAGATGTTAATGGCAAGAAGAGTAAGCTAATTGACATAACCTTGGAAGATACTGAGGAATGTCTTATTTCTCAAAGCCTGCAAGGTACATCTTCATGGAAATTCAGAAGATTGATGGCAATTATTATCCTGAGCTGTACGGATGAAGAGTTGGCAGTTCCGTCCGACGAGGATCAAAATGCATGTGCATTATGTGGAGAACCTTTTGACGTGTCTTACAGTGATGAAACGGAGAACTGGATGTGCAGAGGAGCTGTATACCTTAAAGCGCCGAATGAAATAACAACAGGCATGGACAGGTCGCTGTTAGGCCCCATTATTCATGCTAAATGCAGATCTTAA

Protein sequence

>MsG0780036912.01.T01

MFKLNLIDRTKFTKIDESVIPVNHFDFVSFKDILQSNREDKHVDVIGHIIEKDVLTEKDVNGKKSKLIDITLEDTEECLISQSLQGTSSWKFRRLMAIIILSCTDEELAVPSDEDQNACALCGEPFDVSYSDETENWMCRGAVYLKAPNEITTGMDRSLLGPIIHAKCRS*