Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011446.01.T01 | AES68229.1 | 62.933 | 375 | 45 | 10 | 18 | 340 | 23 | 355 | 6.83E-140 | 414 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0280011446.01 | 0.873378 | 1.540575e-67 | 6.671074e-64 |
| MsG0080047912.01 | MsG0280011446.01 | 0.800427 | 1.448858e-48 | 6.744600e-46 |
| MsG0080047940.01 | MsG0280011446.01 | 0.801726 | 7.859847e-49 | 3.780843e-46 |
| MsG0080047944.01 | MsG0280011446.01 | 0.809412 | 1.916201e-50 | 1.124348e-47 |
| MsG0080047957.01 | MsG0280011446.01 | 0.805342 | 1.398050e-49 | 7.378159e-47 |
| MsG0080047959.01 | MsG0280011446.01 | 0.832291 | 1.028109e-55 | 1.143974e-52 |
| MsG0080048035.01 | MsG0280011446.01 | 0.803561 | 3.287809e-49 | 1.657529e-46 |
| MsG0080048462.01 | MsG0280011446.01 | 0.810724 | 9.997147e-51 | 6.072497e-48 |
| MsG0080048618.01 | MsG0280011446.01 | 0.823758 | 1.165787e-53 | 1.013011e-50 |
| MsG0080048764.01 | MsG0280011446.01 | 0.814752 | 1.312997e-51 | 8.884811e-49 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011446.01.T01 | MTR_2g104600 | 62.933 | 375 | 45 | 10 | 18 | 340 | 23 | 355 | 8.27e-144 | 414 |
| MsG0280011446.01.T01 | MTR_2g104600 | 63.158 | 342 | 82 | 11 | 18 | 340 | 23 | 339 | 1.39e-129 | 377 |
| MsG0280011446.01.T01 | MTR_4g114970 | 41.034 | 290 | 123 | 8 | 62 | 340 | 142 | 394 | 2.23e-59 | 199 |
| MsG0280011446.01.T01 | MTR_4g114970 | 40.702 | 285 | 121 | 8 | 62 | 335 | 142 | 389 | 6.76e-58 | 193 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011446.01.T01 | AT2G26000 | 51.890 | 291 | 94 | 6 | 61 | 342 | 144 | 397 | 2.13e-87 | 271 |
| MsG0280011446.01.T01 | AT2G26000 | 51.890 | 291 | 94 | 6 | 61 | 342 | 144 | 397 | 2.13e-87 | 271 |
| MsG0280011446.01.T01 | AT2G26000 | 47.423 | 291 | 89 | 7 | 61 | 342 | 144 | 379 | 3.88e-74 | 236 |
| MsG0280011446.01.T01 | AT2G42160 | 41.096 | 292 | 119 | 7 | 62 | 340 | 152 | 403 | 1.16e-63 | 209 |
| MsG0280011446.01.T01 | AT2G42160 | 41.096 | 292 | 119 | 7 | 62 | 340 | 152 | 403 | 1.91e-63 | 209 |
| MsG0280011446.01.T01 | AT2G42160 | 40.690 | 290 | 119 | 7 | 62 | 338 | 152 | 401 | 4.05e-62 | 204 |
Find 122 sgRNAs with CRISPR-Local
Find 281 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCATCTTCTAACACAC+TGG | 0.592634 | 2:+84104676 | MsG0280011446.01.T01:three_prime_UTR |
| AAACCACCACTGCTAGTTCG+AGG | 0.555116 | 2:+84104509 | MsG0280011446.01.T01:three_prime_UTR |
| AAACCCCTCCTCATCGCTTC+CGG | 0.484421 | 2:+84101026 | MsG0280011446.01.T01:CDS |
| AAAGGAGAGGGACATGAACG+AGG | 0.707875 | 2:+84104481 | MsG0280011446.01.T01:three_prime_UTR |
| AACCACCACTGCTAGTTCGA+GGG | 0.616800 | 2:+84104510 | MsG0280011446.01.T01:three_prime_UTR |
| AACCTCATGGTTTGTCTTGA+GGG | 0.281710 | 2:+84104300 | MsG0280011446.01.T01:three_prime_UTR |
| AACTAACTTCCCATCAGTTT+TGG | 0.267305 | 2:-84102961 | None:intergenic |
| AAGAAAAGCATGGTAGAAAA+AGG | 0.322143 | 2:+84104423 | MsG0280011446.01.T01:three_prime_UTR |
| AAGCGTGTCTGGGATTATGT+CGG | 0.621807 | 2:+84102909 | MsG0280011446.01.T01:CDS |
| AAGGGACATGCAATGTACCA+AGG | 0.675542 | 2:-84101765 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATCATCTTCTAACACAC+TGG | + | Chr2:84104676-84104695 | MsG0280011446.01.T01:three_prime_UTR | 30.0% | |
| ACAAAGACATGGTTATAACT+TGG | - | Chr2:84103633-84103652 | None:intergenic | 30.0% | |
| ACCAAATCAAATGACTTATG+CGG | + | Chr2:84101166-84101185 | MsG0280011446.01.T01:intron | 30.0% | |
| AGAATTTAACAAAAGGGTTG+GGG | - | Chr2:84102730-84102749 | None:intergenic | 30.0% | |
| AGCATATGACAATTACAAAG+TGG | - | Chr2:84103996-84104015 | None:intergenic | 30.0% | |
| ATACCTGAAAATATAAGCAG+GGG | - | Chr2:84102590-84102609 | None:intergenic | 30.0% | |
| CACATATAACACATATCCAA+AGG | - | Chr2:84102671-84102690 | None:intergenic | 30.0% | |
| CATACCTGAAAATATAAGCA+GGG | - | Chr2:84102591-84102610 | None:intergenic | 30.0% | |
| CTTGATTCAGATATAAAGGA+GGG | + | Chr2:84102829-84102848 | MsG0280011446.01.T01:intron | 30.0% | |
| CTTTAATGTTTATACCAGAC+AGG | - | Chr2:84103395-84103414 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 84100836 | 84104891 | 84100836 | ID=MsG0280011446.01;Name=MsG0280011446.01 |
| Chr2 | mRNA | 84100836 | 84104891 | 84100836 | ID=MsG0280011446.01.T01;Parent=MsG0280011446.01;Name=MsG0280011446.01.T01;_AED=0.43;_eAED=0.44;_QI=54|0.63|0.75|1|0.81|0.75|12|665|349 |
| Chr2 | exon | 84100836 | 84101047 | 84100836 | ID=MsG0280011446.01.T01:exon:3206;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84101131 | 84101153 | 84101131 | ID=MsG0280011446.01.T01:exon:3207;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84101686 | 84101856 | 84101686 | ID=MsG0280011446.01.T01:exon:3208;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84102249 | 84102345 | 84102249 | ID=MsG0280011446.01.T01:exon:3209;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84102508 | 84102520 | 84102508 | ID=MsG0280011446.01.T01:exon:3210;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84102606 | 84102709 | 84102606 | ID=MsG0280011446.01.T01:exon:3211;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84102839 | 84103073 | 84102839 | ID=MsG0280011446.01.T01:exon:3212;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84103291 | 84103344 | 84103291 | ID=MsG0280011446.01.T01:exon:3213;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84103440 | 84103583 | 84103440 | ID=MsG0280011446.01.T01:exon:3214;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84103814 | 84103876 | 84103814 | ID=MsG0280011446.01.T01:exon:3215;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84104093 | 84104147 | 84104093 | ID=MsG0280011446.01.T01:exon:3216;Parent=MsG0280011446.01.T01 |
| Chr2 | exon | 84104294 | 84104891 | 84104294 | ID=MsG0280011446.01.T01:exon:3217;Parent=MsG0280011446.01.T01 |
| Chr2 | five_prime_UTR | 84100836 | 84100889 | 84100836 | ID=MsG0280011446.01.T01:five_prime_utr;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84100890 | 84101047 | 84100890 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84101131 | 84101153 | 84101131 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84101686 | 84101856 | 84101686 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84102249 | 84102345 | 84102249 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84102508 | 84102520 | 84102508 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84102606 | 84102709 | 84102606 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84102839 | 84103073 | 84102839 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84103291 | 84103344 | 84103291 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84103440 | 84103583 | 84103440 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | CDS | 84103814 | 84103864 | 84103814 | ID=MsG0280011446.01.T01:cds;Parent=MsG0280011446.01.T01 |
| Chr2 | three_prime_UTR | 84103865 | 84103876 | 84103865 | ID=MsG0280011446.01.T01:three_prime_utr;Parent=MsG0280011446.01.T01 |
| Chr2 | three_prime_UTR | 84104093 | 84104147 | 84104093 | ID=MsG0280011446.01.T01:three_prime_utr;Parent=MsG0280011446.01.T01 |
| Chr2 | three_prime_UTR | 84104294 | 84104891 | 84104294 | ID=MsG0280011446.01.T01:three_prime_utr;Parent=MsG0280011446.01.T01 |
| Gene Sequence |
>MsG0280011446.01.T01 ATGTGGAGTTCAGCAGAGTCGTCTCCGAAGAAGAACAAACTGAATCTTCTTCTTCTCAACTTCTTTTCAATTTCTCTTCTTGGTAACCCTAGAATCGGAAAGAAACTCGCGGGCCTCATGCATGTCTTCCCCGAACAAACCCCTCCTCATCGCTTCCGGTTGGACGGAAACCCTGGCGTGTATGTGCAGTATACTGGGTCAAATGAACACGCACAACCATCCAATGCTACTTCAACTGAACAGCCTACATGTCCAGTTTGCCTTGGTACATTGCATGTCCCTTTTTCGCTTAGGCTTATGTACTACAGCTTACCTTCTAATGTAAGTAGTTATAGATGCAAGCTGAGAAAGGAGCGATTAGATCAAGACACGGTGGAATTCTTACAACCATATGCCATCATTCTTTCCATTGCTCCTGTATTTCGAAATGGGCAGATTCTTCTTGTCCTCTGGGATTTCTTGGTATGCCGCTATTGCCAACAACAGCTGAAAATTCTATATGTTCGTTTGTCAAACACTGAGAACCTTTGGATATGTGTTATATGTGGGTTTGTTGGCTGTGGAAGATATAAAGGAGGGCATGCCATAATACACTGGAAAGAAACGCAGCATTGCTATTCCTTAGAAGTGGAAACTAAGCGTGTCTGGGATTATGTCGGAGACAACTATGTCCATAGACTTATTCAGTCCAAAACTGATGGGAAGTTAGTTGAGCTGAACTCTCACTGTGTGCATGCAGACAGTGGCAGCTGTGGAGATAATGCCATGAGAGAGGCTATACTTAATAGTAAAGTTGAAGCGATTGTCAATGAATACAATGAATTGCTTGCTACACAACTTGAAAACCAAAAATTGTATTTTGAATCCTTATTACAACAAGTAGAACAAGAAACTGAAGGAAAAATTTCCGTAGCAGTTCAGAAGGCTGTCAGTCTCAAACAGCATAAGATTCAGGCGAAGATCGATCGATGTAACAAAGAGAAGAAGTTTCTGGATGAGCTTAATGACAATCTTTTGAAAACGAGGAGATCTGGAAGCCAAGTTACTTGA |
| Protein sequence |
>MsG0280011446.01.T01 MWSSAESSPKKNKLNLLLLNFFSISLLGNPRIGKKLAGLMHVFPEQTPPHRFRLDGNPGVYVQYTGSNEHAQPSNATSTEQPTCPVCLGTLHVPFSLRLMYYSLPSNVSSYRCKLRKERLDQDTVEFLQPYAIILSIAPVFRNGQILLVLWDFLVCRYCQQQLKILYVRLSNTENLWICVICGFVGCGRYKGGHAIIHWKETQHCYSLEVETKRVWDYVGDNYVHRLIQSKTDGKLVELNSHCVHADSGSCGDNAMREAILNSKVEAIVNEYNELLATQLENQKLYFESLLQQVEQETEGKISVAVQKAVSLKQHKIQAKIDRCNKEKKFLDELNDNLLKTRRSGSQVT* |
