Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036966.01.T01 | AAV98051.1 | 100 | 649 | 0 | 0 | 1 | 649 | 1 | 649 | 0 | 1332 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780036953.01 | MsG0780036966.01 | 0.812414 | 4.291087e-51 | 2.726726e-48 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036966.01.T01 | MTR_7g024580 | 99.692 | 649 | 2 | 0 | 1 | 649 | 1 | 649 | 0.0 | 1328 |
| MsG0780036966.01.T01 | MTR_7g024390 | 93.683 | 649 | 40 | 1 | 1 | 649 | 1 | 648 | 0.0 | 1243 |
| MsG0780036966.01.T01 | MTR_7g099680 | 92.661 | 654 | 38 | 3 | 1 | 649 | 1 | 649 | 0.0 | 1241 |
| MsG0780036966.01.T01 | MTR_4g063710 | 92.296 | 649 | 50 | 0 | 1 | 649 | 1 | 649 | 0.0 | 1230 |
| MsG0780036966.01.T01 | MTR_4g063720 | 91.988 | 649 | 52 | 0 | 1 | 649 | 1 | 649 | 0.0 | 1226 |
| MsG0780036966.01.T01 | MTR_4g130540 | 86.697 | 654 | 81 | 2 | 1 | 649 | 1 | 653 | 0.0 | 1147 |
| MsG0780036966.01.T01 | MTR_6g033850 | 66.829 | 615 | 203 | 1 | 1 | 615 | 1 | 614 | 0.0 | 846 |
| MsG0780036966.01.T01 | MTR_7g026030 | 65.079 | 630 | 215 | 4 | 1 | 628 | 1 | 627 | 0.0 | 832 |
| MsG0780036966.01.T01 | MTR_7g025760 | 65.217 | 621 | 212 | 3 | 1 | 619 | 1 | 619 | 0.0 | 831 |
| MsG0780036966.01.T01 | MTR_6g033760 | 65.909 | 616 | 209 | 1 | 1 | 616 | 1 | 615 | 0.0 | 831 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036966.01.T01 | AT5G02500 | 91.590 | 654 | 47 | 2 | 1 | 649 | 1 | 651 | 0.0 | 1226 |
| MsG0780036966.01.T01 | AT3G12580 | 90.992 | 655 | 48 | 3 | 1 | 649 | 1 | 650 | 0.0 | 1204 |
| MsG0780036966.01.T01 | AT3G09440 | 90.337 | 652 | 57 | 3 | 1 | 649 | 1 | 649 | 0.0 | 1204 |
| MsG0780036966.01.T01 | AT3G09440 | 90.337 | 652 | 57 | 3 | 1 | 649 | 1 | 649 | 0.0 | 1204 |
| MsG0780036966.01.T01 | AT3G09440 | 90.337 | 652 | 57 | 3 | 1 | 649 | 1 | 649 | 0.0 | 1204 |
| MsG0780036966.01.T01 | AT3G09440 | 90.337 | 652 | 57 | 3 | 1 | 649 | 1 | 649 | 0.0 | 1204 |
| MsG0780036966.01.T01 | AT5G02490 | 89.466 | 655 | 61 | 2 | 1 | 649 | 1 | 653 | 0.0 | 1201 |
| MsG0780036966.01.T01 | AT1G56410 | 88.045 | 619 | 72 | 1 | 1 | 619 | 1 | 617 | 0.0 | 1123 |
| MsG0780036966.01.T01 | AT1G16030 | 83.359 | 649 | 105 | 3 | 1 | 649 | 1 | 646 | 0.0 | 1112 |
| MsG0780036966.01.T01 | AT5G28540 | 65.635 | 614 | 205 | 4 | 7 | 618 | 35 | 644 | 0.0 | 825 |
Find 178 sgRNAs with CRISPR-Local
Find 220 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTTGCTCCTTCTTGGT+TGG | 0.457926 | 7:+17240800 | None:intergenic |
| AAAATCAGTTCAAAGCTTTC+TGG | 0.170451 | 7:-17240392 | MsG0780036966.01.T01:CDS |
| AAAATCTTCACCACCAAGAT+GGG | 0.521512 | 7:+17241384 | None:intergenic |
| AAACAAAGAATCAATCTCAA+TGG | 0.529138 | 7:+17241219 | None:intergenic |
| AAAGATGCTGGTGTTATCGC+TGG | 0.515111 | 7:-17241592 | MsG0780036966.01.T01:CDS |
| AAAGGAAGAAATCGAGAAGA+TGG | 0.524070 | 7:-17240528 | MsG0780036966.01.T01:CDS |
| AAAGGTGAAGGTCCTGCGAT+TGG | 0.523782 | 7:-17242791 | MsG0780036966.01.T01:CDS |
| AAATCTGGCACGGGTGATTG+TGG | 0.583692 | 7:+17241177 | None:intergenic |
| AACCAAGGATAACAACTTGT+TGG | 0.482428 | 7:-17240714 | MsG0780036966.01.T01:CDS |
| AACGTGTTGATCTTTGATTT+GGG | 0.450143 | 7:-17241481 | MsG0780036966.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATCAGTTCAAAGCTTTC+TGG | - | Chr7:17242497-17242516 | MsG0780036966.01.T01:intron | 30.0% | |
| AAAATTACCAAAAACGGTGT+TGG | + | Chr7:17240291-17240310 | None:intergenic | 30.0% | |
| AAGAACAAGAAGGATATTAG+TGG | - | Chr7:17241570-17241589 | MsG0780036966.01.T01:CDS | 30.0% | |
| AGTAACAACAGCATTCTTAA+TGG | + | Chr7:17241238-17241257 | None:intergenic | 30.0% | |
| TCACTTCTTACTATTGAAGA+GGG | - | Chr7:17241450-17241469 | MsG0780036966.01.T01:CDS | 30.0% | |
| TCAGTAAAAGCAACATAAGA+CGG | + | Chr7:17240215-17240234 | None:intergenic | 30.0% | |
| TGTTCTTGTGATTATAGATC+TGG | - | Chr7:17240343-17240362 | MsG0780036966.01.T01:CDS | 30.0% | |
| TTGTAGTTAACACCAATCAT+TGG | + | Chr7:17241126-17241145 | None:intergenic | 30.0% | |
| TTTGTATGTCTGTTTAGAAG+GGG | - | Chr7:17240902-17240921 | MsG0780036966.01.T01:CDS | 30.0% | |
| AAAATCTTCACCACCAAGAT+GGG | + | Chr7:17241508-17241527 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 17239908 | 17243003 | 17239908 | ID=MsG0780036966.01;Name=MsG0780036966.01 |
| Chr7 | mRNA | 17239908 | 17243003 | 17239908 | ID=MsG0780036966.01.T01;Parent=MsG0780036966.01;Name=MsG0780036966.01.T01;_AED=0.48;_eAED=0.48;_QI=181|1|1|1|1|1|2|243|649 |
| Chr7 | exon | 17239908 | 17241886 | 17239908 | ID=MsG0780036966.01.T01:exon:11579;Parent=MsG0780036966.01.T01 |
| Chr7 | exon | 17242609 | 17243003 | 17242609 | ID=MsG0780036966.01.T01:exon:11578;Parent=MsG0780036966.01.T01 |
| Chr7 | five_prime_UTR | 17242823 | 17243003 | 17242823 | ID=MsG0780036966.01.T01:five_prime_utr;Parent=MsG0780036966.01.T01 |
| Chr7 | CDS | 17242609 | 17242822 | 17242609 | ID=MsG0780036966.01.T01:cds;Parent=MsG0780036966.01.T01 |
| Chr7 | CDS | 17240151 | 17241886 | 17240151 | ID=MsG0780036966.01.T01:cds;Parent=MsG0780036966.01.T01 |
| Chr7 | three_prime_UTR | 17239908 | 17240150 | 17239908 | ID=MsG0780036966.01.T01:three_prime_utr;Parent=MsG0780036966.01.T01 |
| Gene Sequence |
>MsG0780036966.01.T01 ATGGCTGGTAAAGGTGAAGGTCCTGCGATTGGAATAGATCTGGGAACAACATACTCGTGTGTTGGAGTATGGCAACACGATCGTGTTGAGATCATCGCCAATGATCAAGGAAACCGAACGACGCCGTCTTATGTTGCTTTTACTGATAGTGAAAGATTGATCGGTGATGCTGCTAAGAATCAGGTCGCGATGAATCCAACCAACACCGTTTTTGATGCAAAGAGGTTGATTGGTAGGAGAATTTCTGATGCTTCTGTTCAGAGTGACATGAAGTTGTGGCCTTTTAAGGTTACCGCTGGTCCTGGTGAAAAGCCAATGATTGGTGTTAACTACAAGGGTGAGGAAAAACTGTTTGCATCTGAGGAAATCTCATCCATGGTTTTGATTAAGATGCGTGAGATTGCCGAAGCTTATCTTGGTGTCACCATTAAGAATGCTGTTGTTACTGTCCCTGCTTACTTCAACGATTCTCAGCGTCAAGCAACAAAAGATGCTGGTGTTATCGCTGGTTTGAATGTTTTGAGAATTATTAATGAGCCAACTGCTGCTGCTATTGCATACGGTCTTGACAAAAAGGCAACAAGTGTTGGTGAGAAGAACGTGTTGATCTTTGATTTGGGAGGTGGTACTTTTGATGTGTCACTTCTTACTATTGAAGAGGGTATTTTTGAAGTTAAGGCCACTGCTGGTGATACCCATCTTGGTGGTGAAGATTTTGATAACAGAATGGTTAACCATTTTGTTCAAGAGTTTAAGAGGAAGAACAAGAAGGATATTAGTGGTAACCCTAGAGCACTTAGGAGATTGAGAACTGCATGTGAGAGAGCGAAGAGAACTCTCTCATCTACTGCACAAACTACCATTGAGATTGATTCTTTGTTTGAGGGTGTTGATTTTTACACCACAATCACCCGTGCCAGATTTGAGGAACTTAACATGGATCTGTTTAGAAAGTGTATGGAGCCTGTAGAGAAGTGTTTGAGAGATGCTAAGATGGACAAAAGCACTGTTCATGATGTTGTTCTTGTTGGTGGTTCAACTAGAATTCCCAAAGTTCAGCAATTGTTGCAAGACTTTTTCAATGGTAAGGAGCTTTGCAAAAGCATCAACCCCGACGAGGCTGTTGCTTATGGTGCCGCCGTCCAAGCTGCTATATTGAGTGGTGAAGGAAACGAGAAGGTTCAAGATTTGTTGCTTTTGGATGTCACCCCATTGTCTCAAGGTTTGGAGACTGCAGGAGGTGTCATGACTGTATTGATTCCAAGGAACACTACCATTCCAACCAAGAAGGAGCAAGTTTTCTCTACTTATTCAGACAACCAACCTGGTGTGTTGATCCAAGTCTATGAAGGTGAGAGAACCAGAACCAAGGATAACAACTTGTTGGGTAAGTTTGAGCTTTCTGGAATTCCTCCAGCACCCCGTGGTGTTCCTCAGATCACTGTTTGTTTTGATATTGATGCCAATGGTATCTTAAACGTATCTGCTGAGGACAAAACTACTGGGCAGAAGAACAAGATTACCATCACCAACGACAAGGGAAGGTTGTCAAAGGAAGAAATCGAGAAGATGGTTCAAGAAGCAGAGAAATACAAGAGTGAAGATGAGGAGCACAAGAAGAAGGTTGAGGCAAAGAACTCCTTGGAGAACTATGCATACAACATGAGAAACACCATTAAAGACGAGAAAATCAGTTCAAAGCTTTCTGGAGGAGACAAGAAGCAGATTGAGGATGCAATTGAGGGAGCCATTCAATGGCTTGATGCCAACCAGCTTGCTGAAGCCGATGAGTTTGAAGACAAGATGAAGGAGCTTGAAACCATCTGCAACCCAATCATTGCAAAGATGTACCAAGGTGGAGCTGGTGAAGGTCCAGAAGTTGATGATGATGCTGCTCCACCTCCAAGTGGCAGTGGCGGTGCTGGTCCCAAGATTGAGGAGGTCGACTAA |
| Protein sequence |
>MsG0780036966.01.T01 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPTNTVFDAKRLIGRRISDASVQSDMKLWPFKVTAGPGEKPMIGVNYKGEEKLFASEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEGVDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSQGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTKDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKYKSEDEEHKKKVEAKNSLENYAYNMRNTIKDEKISSKLSGGDKKQIEDAIEGAIQWLDANQLAEADEFEDKMKELETICNPIIAKMYQGGAGEGPEVDDDAAPPPSGSGGAGPKIEEVD* |
