Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036976.01.T01 | XP_003621898.1 | 95.082 | 183 | 5 | 2 | 1 | 183 | 1 | 179 | 7.08E-115 | 333 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036976.01.T01 | C6T1Z6 | 70.414 | 169 | 43 | 2 | 14 | 182 | 7 | 168 | 9.79E-58 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036976.01.T01 | G7KXV1 | 95.082 | 183 | 5 | 2 | 1 | 183 | 1 | 179 | 3.38e-115 | 333 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015629.01 | MsG0780036976.01 | 0.804670 | 1.932133e-49 | 1.002203e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036976.01.T01 | MTR_7g024750 | 95.082 | 183 | 5 | 2 | 1 | 183 | 1 | 179 | 8.58e-119 | 333 |
| MsG0780036976.01.T01 | MTR_7g024730 | 84.337 | 166 | 26 | 0 | 18 | 183 | 21 | 186 | 3.85e-88 | 256 |
| MsG0780036976.01.T01 | MTR_6g073090 | 31.138 | 167 | 100 | 3 | 28 | 182 | 10 | 173 | 1.23e-15 | 71.2 |
| MsG0780036976.01.T01 | MTR_6g073040 | 31.138 | 167 | 100 | 3 | 28 | 182 | 10 | 173 | 1.23e-15 | 71.2 |
| MsG0780036976.01.T01 | MTR_4g106940 | 28.324 | 173 | 112 | 4 | 7 | 179 | 123 | 283 | 8.97e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036976.01.T01 | AT2G39530 | 46.358 | 151 | 71 | 3 | 38 | 182 | 27 | 173 | 3.55e-34 | 119 |
| MsG0780036976.01.T01 | AT2G39518 | 44.371 | 151 | 76 | 2 | 37 | 182 | 26 | 173 | 1.25e-32 | 115 |
Find 34 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGCTAGTCTTCTTCTATT+TGG | 0.151858 | 7:+17430154 | MsG0780036976.01.T01:CDS |
| CTTTCCAAAGAATGAGTTTA+AGG | 0.157069 | 7:-17430122 | None:intergenic |
| ATGCTCTCAACAATAGTAAT+TGG | 0.185495 | 7:+17429502 | MsG0780036976.01.T01:CDS |
| ATATCATACTTTCTACTTTC+CGG | 0.240187 | 7:+17430037 | MsG0780036976.01.T01:CDS |
| TTTCCCTTAAACTCATTCTT+TGG | 0.247502 | 7:+17430118 | MsG0780036976.01.T01:CDS |
| TCCGGTTCAGCTGCTGGATT+TGG | 0.285065 | 7:+17430055 | MsG0780036976.01.T01:CDS |
| GCATCAAACTAAGCTTTCTT+TGG | 0.298965 | 7:-17430223 | None:intergenic |
| TCAGGAAATCGTGTATTAAA+TGG | 0.322290 | 7:+17429574 | MsG0780036976.01.T01:CDS |
| CTACTTTCCGGTTCAGCTGC+TGG | 0.331360 | 7:+17430049 | MsG0780036976.01.T01:CDS |
| GAACTGTCCTTCTCCTCTTA+AGG | 0.349079 | 7:+17429082 | MsG0780036976.01.T01:CDS |
| TCAAAGATATCCATGCTTAT+AGG | 0.351862 | 7:+17429199 | MsG0780036976.01.T01:CDS |
| AAATTGAAAGTGCCATTTGC+AGG | 0.383840 | 7:-17429540 | None:intergenic |
| ACCAAATCCAGCAGCTGAAC+CGG | 0.388148 | 7:-17430056 | None:intergenic |
| TTTCCAAAGAATGAGTTTAA+GGG | 0.388517 | 7:-17430121 | None:intergenic |
| CATATATTCTTCAAAGCAAA+TGG | 0.394531 | 7:+17430094 | MsG0780036976.01.T01:CDS |
| AACTGTCCTTCTCCTCTTAA+GGG | 0.398817 | 7:+17429083 | MsG0780036976.01.T01:CDS |
| CTTGAAACTGTTGAACTTGA+GGG | 0.435851 | 7:-17429060 | None:intergenic |
| TGCTCTCAACAATAGTAATT+GGG | 0.436051 | 7:+17429503 | MsG0780036976.01.T01:CDS |
| TGCATACAATTGCCTGCAAA+TGG | 0.440380 | 7:+17429528 | MsG0780036976.01.T01:CDS |
| CAAAAGTTAACACCCTTAAG+AGG | 0.485567 | 7:-17429095 | None:intergenic |
| CTTAAACTCATTCTTTGGAA+AGG | 0.491531 | 7:+17430123 | MsG0780036976.01.T01:CDS |
| GAAACTTTAGACACAGATGA+TGG | 0.498154 | 7:+17429159 | MsG0780036976.01.T01:CDS |
| CGTGTATTAAATGGTGGTGC+AGG | 0.498327 | 7:+17429583 | MsG0780036976.01.T01:CDS |
| CATAATCATTGTCTTAACAA+AGG | 0.527957 | 7:+17429137 | MsG0780036976.01.T01:CDS |
| TGAAGTGAAAACTGATGCTA+TGG | 0.542731 | 7:-17430191 | None:intergenic |
| AAACTTTAGACACAGATGAT+GGG | 0.548549 | 7:+17429160 | MsG0780036976.01.T01:CDS |
| TCTTGAAACTGTTGAACTTG+AGG | 0.553543 | 7:-17429061 | None:intergenic |
| CATATGCTCACCTATAAGCA+TGG | 0.583423 | 7:-17429209 | None:intergenic |
| TTAACACCCTTAAGAGGAGA+AGG | 0.587105 | 7:-17429089 | None:intergenic |
| AACTTTAGACACAGATGATG+GGG | 0.609470 | 7:+17429161 | MsG0780036976.01.T01:CDS |
| GGAAATCGTGTATTAAATGG+TGG | 0.612889 | 7:+17429577 | MsG0780036976.01.T01:CDS |
| GAAAACTGATGCTATGGCTG+TGG | 0.617239 | 7:-17430185 | None:intergenic |
| TGATTATGAAAGCAATGAGA+AGG | 0.621556 | 7:-17429122 | None:intergenic |
| GAATATATGCAAATCTTCTG+AGG | 0.629688 | 7:-17430080 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAAAAAAAAAACATATAT+AGG | - | Chr7:17429968-17429987 | None:intergenic | 10.0% |
| !! | TATTGATAAAATTATAGTAT+TGG | + | Chr7:17429367-17429386 | MsG0780036976.01.T01:intron | 10.0% |
| !! | TTTATATCTAGTTAAAAAAT+TGG | - | Chr7:17429302-17429321 | None:intergenic | 10.0% |
| !! | ATGACTTTCTATAATATATT+TGG | + | Chr7:17429673-17429692 | MsG0780036976.01.T01:intron | 15.0% |
| !!! | AACTAAATTTGTTTTTTTCT+TGG | + | Chr7:17430007-17430026 | MsG0780036976.01.T01:intron | 15.0% |
| !!! | CAAGTTTTTTTACTTTATTT+TGG | + | Chr7:17429804-17429823 | MsG0780036976.01.T01:intron | 15.0% |
| !!! | GTTATTTTTACGTTTTATTT+TGG | + | Chr7:17429721-17429740 | MsG0780036976.01.T01:intron | 15.0% |
| !! | AGATGCAAAATAAATATTGA+AGG | - | Chr7:17429442-17429461 | None:intergenic | 20.0% |
| !! | ATATACTTGAAGACTTATTA+TGG | - | Chr7:17429650-17429669 | None:intergenic | 20.0% |
| !! | TATACTTGAAGACTTATTAT+GGG | - | Chr7:17429649-17429668 | None:intergenic | 20.0% |
| !!! | AATGTAACAAAGTTTTTCTA+AGG | + | Chr7:17429892-17429911 | MsG0780036976.01.T01:intron | 20.0% |
| !!! | AGAAAAACTTTGTTACATTT+TGG | - | Chr7:17429891-17429910 | None:intergenic | 20.0% |
| !!! | ATATTAACTAGCTACAATTT+TGG | + | Chr7:17429467-17429486 | MsG0780036976.01.T01:intron | 20.0% |
| !!! | TATTAACTAGCTACAATTTT+GGG | + | Chr7:17429468-17429487 | MsG0780036976.01.T01:intron | 20.0% |
| ! | AACATATATAGGCTAAACTA+TGG | - | Chr7:17429957-17429976 | None:intergenic | 25.0% |
| ! | ATATCATACTTTCTACTTTC+CGG | + | Chr7:17430037-17430056 | MsG0780036976.01.T01:CDS | 25.0% |
| ! | CAAAGTAGAATAGAAAAACT+TGG | - | Chr7:17429748-17429767 | None:intergenic | 25.0% |
| ! | CATAATCATTGTCTTAACAA+AGG | + | Chr7:17429137-17429156 | MsG0780036976.01.T01:CDS | 25.0% |
| ! | CATATATTCTTCAAAGCAAA+TGG | + | Chr7:17430094-17430113 | MsG0780036976.01.T01:CDS | 25.0% |
| ! | TTATCAATAATTACTTCCTC+CGG | - | Chr7:17429356-17429375 | None:intergenic | 25.0% |
| ! | TTTCCAAAGAATGAGTTTAA+GGG | - | Chr7:17430124-17430143 | None:intergenic | 25.0% |
| !! | TGTCTAAAATTTGTACCTTA+AGG | - | Chr7:17429832-17429851 | None:intergenic | 25.0% |
| !!! | GGCTATAAGTTTGATTTTTA+TGG | + | Chr7:17429604-17429623 | MsG0780036976.01.T01:CDS | 25.0% |
| !!! | GTTTGATTTTTATGGTGATA+AGG | + | Chr7:17429612-17429631 | MsG0780036976.01.T01:CDS | 25.0% |
| !!! | TACTTTATTTTGGTTCCTTA+AGG | + | Chr7:17429814-17429833 | MsG0780036976.01.T01:intron | 25.0% |
| AAACTTTAGACACAGATGAT+GGG | + | Chr7:17429160-17429179 | MsG0780036976.01.T01:CDS | 30.0% | |
| ATGCTCTCAACAATAGTAAT+TGG | + | Chr7:17429502-17429521 | MsG0780036976.01.T01:CDS | 30.0% | |
| CTTTCCAAAGAATGAGTTTA+AGG | - | Chr7:17430125-17430144 | None:intergenic | 30.0% | |
| GAATATATGCAAATCTTCTG+AGG | - | Chr7:17430083-17430102 | None:intergenic | 30.0% | |
| GACCAGAATATCTCATAAAT+AGG | - | Chr7:17429257-17429276 | None:intergenic | 30.0% | |
| GTCCTATTTATGAGATATTC+TGG | + | Chr7:17429252-17429271 | MsG0780036976.01.T01:intron | 30.0% | |
| TCAAAGATATCCATGCTTAT+AGG | + | Chr7:17429199-17429218 | MsG0780036976.01.T01:CDS | 30.0% | |
| TCAGGAAATCGTGTATTAAA+TGG | + | Chr7:17429574-17429593 | MsG0780036976.01.T01:CDS | 30.0% | |
| TGATTATGAAAGCAATGAGA+AGG | - | Chr7:17429125-17429144 | None:intergenic | 30.0% | |
| TGCTCTCAACAATAGTAATT+GGG | + | Chr7:17429503-17429522 | MsG0780036976.01.T01:CDS | 30.0% | |
| TTTCCCTTAAACTCATTCTT+TGG | + | Chr7:17430118-17430137 | MsG0780036976.01.T01:CDS | 30.0% | |
| ! | CTTAAACTCATTCTTTGGAA+AGG | + | Chr7:17430123-17430142 | MsG0780036976.01.T01:CDS | 30.0% |
| AACTTTAGACACAGATGATG+GGG | + | Chr7:17429161-17429180 | MsG0780036976.01.T01:CDS | 35.0% | |
| CAAAAGTTAACACCCTTAAG+AGG | - | Chr7:17429098-17429117 | None:intergenic | 35.0% | |
| CTTGAAACTGTTGAACTTGA+GGG | - | Chr7:17429063-17429082 | None:intergenic | 35.0% | |
| GAAACTTTAGACACAGATGA+TGG | + | Chr7:17429159-17429178 | MsG0780036976.01.T01:CDS | 35.0% | |
| GGAAATCGTGTATTAAATGG+TGG | + | Chr7:17429577-17429596 | MsG0780036976.01.T01:CDS | 35.0% | |
| TAGATGTCGCTAAATAAGAC+CGG | + | Chr7:17429334-17429353 | MsG0780036976.01.T01:intron | 35.0% | |
| TCTTGAAACTGTTGAACTTG+AGG | - | Chr7:17429064-17429083 | None:intergenic | 35.0% | |
| TGAAGTGAAAACTGATGCTA+TGG | - | Chr7:17430194-17430213 | None:intergenic | 35.0% | |
| ! | AGTTACACGCATAGACATTT+TGG | + | Chr7:17429776-17429795 | MsG0780036976.01.T01:intron | 35.0% |
| ! | TCAATTTTCACTGTGGTATC+AGG | + | Chr7:17429556-17429575 | MsG0780036976.01.T01:CDS | 35.0% |
| ! | TGACTACTAACTGCTCTTTT+CGG | + | Chr7:17429230-17429249 | MsG0780036976.01.T01:intron | 35.0% |
| !! | AAATTGAAAGTGCCATTTGC+AGG | - | Chr7:17429543-17429562 | None:intergenic | 35.0% |
| !! | TCTGCTAGTCTTCTTCTATT+TGG | + | Chr7:17430154-17430173 | MsG0780036976.01.T01:CDS | 35.0% |
| AACTGTCCTTCTCCTCTTAA+GGG | + | Chr7:17429083-17429102 | MsG0780036976.01.T01:CDS | 40.0% | |
| CATATGCTCACCTATAAGCA+TGG | - | Chr7:17429212-17429231 | None:intergenic | 40.0% | |
| TGCATACAATTGCCTGCAAA+TGG | + | Chr7:17429528-17429547 | MsG0780036976.01.T01:CDS | 40.0% | |
| TTAACACCCTTAAGAGGAGA+AGG | - | Chr7:17429092-17429111 | None:intergenic | 40.0% | |
| ! | GGCACTTTCAATTTTCACTG+TGG | + | Chr7:17429549-17429568 | MsG0780036976.01.T01:CDS | 40.0% |
| !! | GGTCTTATTTAGCGACATCT+AGG | - | Chr7:17429335-17429354 | None:intergenic | 40.0% |
| ATGTCGCTAAATAAGACCGG+AGG | + | Chr7:17429337-17429356 | MsG0780036976.01.T01:intron | 45.0% | |
| GAAAACTGATGCTATGGCTG+TGG | - | Chr7:17430188-17430207 | None:intergenic | 45.0% | |
| GAACTGTCCTTCTCCTCTTA+AGG | + | Chr7:17429082-17429101 | MsG0780036976.01.T01:CDS | 45.0% | |
| ! | CGTGTATTAAATGGTGGTGC+AGG | + | Chr7:17429583-17429602 | MsG0780036976.01.T01:CDS | 45.0% |
| ACCAAATCCAGCAGCTGAAC+CGG | - | Chr7:17430059-17430078 | None:intergenic | 50.0% | |
| CTACTTTCCGGTTCAGCTGC+TGG | + | Chr7:17430049-17430068 | MsG0780036976.01.T01:CDS | 55.0% | |
| TCCGGTTCAGCTGCTGGATT+TGG | + | Chr7:17430055-17430074 | MsG0780036976.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 17429009 | 17430237 | 17429009 | ID=MsG0780036976.01;Name=MsG0780036976.01 |
| Chr7 | mRNA | 17429009 | 17430237 | 17429009 | ID=MsG0780036976.01.T01;Parent=MsG0780036976.01;Name=MsG0780036976.01.T01;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|1|1|3|0|183 |
| Chr7 | exon | 17429009 | 17429220 | 17429009 | ID=MsG0780036976.01.T01:exon:3237;Parent=MsG0780036976.01.T01 |
| Chr7 | exon | 17429498 | 17429633 | 17429498 | ID=MsG0780036976.01.T01:exon:3238;Parent=MsG0780036976.01.T01 |
| Chr7 | exon | 17430034 | 17430237 | 17430034 | ID=MsG0780036976.01.T01:exon:3239;Parent=MsG0780036976.01.T01 |
| Chr7 | CDS | 17429009 | 17429220 | 17429009 | ID=MsG0780036976.01.T01:cds;Parent=MsG0780036976.01.T01 |
| Chr7 | CDS | 17429498 | 17429633 | 17429498 | ID=MsG0780036976.01.T01:cds;Parent=MsG0780036976.01.T01 |
| Chr7 | CDS | 17430034 | 17430237 | 17430034 | ID=MsG0780036976.01.T01:cds;Parent=MsG0780036976.01.T01 |
| Gene Sequence |
| Protein sequence |
