AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037361.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGGAGGTGGGTCCTTGTT+GGG 0.284599 7:-24264046 MsG0780037361.01.T01:CDS
GAGAGGAGGTGGGTCCTTGT+TGG 0.361058 7:-24264047 MsG0780037361.01.T01:CDS
ATTCACCGTGACTGCTTCTT+CGG 0.383104 7:-24264235 MsG0780037361.01.T01:CDS
TTTGGTGTCGGAATGCTTTG+TGG 0.446845 7:-24264123 MsG0780037361.01.T01:CDS
TGATGATGTGTAGTGTGGTT+TGG 0.449675 7:-24264176 MsG0780037361.01.T01:CDS
GTCTTTATCGTTGGTGATGT+GGG 0.460263 7:-24264259 MsG0780037361.01.T01:CDS
TGTTGTTGATGTGGTGGTGA+TGG 0.464047 7:-24264202 MsG0780037361.01.T01:CDS
TTCATGGTGAGGTGTTTAGC+TGG 0.469397 7:-24264291 MsG0780037361.01.T01:CDS
AGTCTTTATCGTTGGTGATG+TGG 0.483850 7:-24264260 MsG0780037361.01.T01:CDS
GATGTGTAGTGTGGTTTGGA+TGG 0.492277 7:-24264172 MsG0780037361.01.T01:CDS
TGTCGGAATGCTTTGTGGAC+GGG 0.498332 7:-24264118 MsG0780037361.01.T01:CDS
GAGGAGGTGGGTCCTTGTTG+GGG 0.498343 7:-24264045 MsG0780037361.01.T01:CDS
TTTGGGCTCGGATTTGGTGT+CGG 0.516979 7:-24264135 MsG0780037361.01.T01:CDS
TGTTGATGTGGTGGTGATGG+TGG 0.528766 7:-24264199 MsG0780037361.01.T01:CDS
AGTAGTTCAGTCTTTATCGT+TGG 0.531741 7:-24264268 MsG0780037361.01.T01:CDS
GTGTCGGAATGCTTTGTGGA+CGG 0.543195 7:-24264119 MsG0780037361.01.T01:CDS
TCATGGTGTTGTTGATGTGG+TGG 0.554128 7:-24264208 MsG0780037361.01.T01:CDS
ATGTCATGGTGTTGTTGATG+TGG 0.603144 7:-24264211 MsG0780037361.01.T01:CDS
GGTGGTGATGATGTGTAGTG+TGG 0.609001 7:-24264181 MsG0780037361.01.T01:CDS
ACTGCTTCTTCGGAATGTCA+TGG 0.609517 7:-24264225 MsG0780037361.01.T01:CDS
GTGGTTTGGATGGATAGTCG+TGG 0.621078 7:-24264162 MsG0780037361.01.T01:CDS
ACATTCCGAAGAAGCAGTCA+CGG 0.637913 7:+24264230 None:intergenic
TATAGGAAAACACCCCAACA+AGG 0.665609 7:+24264033 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGTAGTTCAGTCTTTATCGT+TGG - Chr7:24264052-24264071 MsG0780037361.01.T01:CDS 35.0%
!!! ATGGATAGTCGTGGATTTTT+TGG - Chr7:24264167-24264186 MsG0780037361.01.T01:CDS 35.0%
!!! TGGATAGTCGTGGATTTTTT+GGG - Chr7:24264168-24264187 MsG0780037361.01.T01:CDS 35.0%
TATAGGAAAACACCCCAACA+AGG + Chr7:24264290-24264309 None:intergenic 40.0%
! TGATGATGTGTAGTGTGGTT+TGG - Chr7:24264144-24264163 MsG0780037361.01.T01:CDS 40.0%
!! AGTCTTTATCGTTGGTGATG+TGG - Chr7:24264060-24264079 MsG0780037361.01.T01:CDS 40.0%
!! ATGTCATGGTGTTGTTGATG+TGG - Chr7:24264109-24264128 MsG0780037361.01.T01:CDS 40.0%
!! GAGCTTTTTAAGGAAGAGGT+TGG - Chr7:24264224-24264243 MsG0780037361.01.T01:CDS 40.0%
!! GTCTTTATCGTTGGTGATGT+GGG - Chr7:24264061-24264080 MsG0780037361.01.T01:CDS 40.0%
!!! TAAGGAAGAGGTTGGTTTTG+TGG - Chr7:24264232-24264251 MsG0780037361.01.T01:CDS 40.0%
ACATTCCGAAGAAGCAGTCA+CGG + Chr7:24264093-24264112 None:intergenic 45.0%
ACTGCTTCTTCGGAATGTCA+TGG - Chr7:24264095-24264114 MsG0780037361.01.T01:CDS 45.0%
ATTCACCGTGACTGCTTCTT+CGG - Chr7:24264085-24264104 MsG0780037361.01.T01:CDS 45.0%
! GATGTGTAGTGTGGTTTGGA+TGG - Chr7:24264148-24264167 MsG0780037361.01.T01:CDS 45.0%
! TGTTGTTGATGTGGTGGTGA+TGG - Chr7:24264118-24264137 MsG0780037361.01.T01:CDS 45.0%
! TTGTGGACGGGAGCTTTTTA+AGG - Chr7:24264214-24264233 MsG0780037361.01.T01:CDS 45.0%
!! ACGGGAGCTTTTTAAGGAAG+AGG - Chr7:24264220-24264239 MsG0780037361.01.T01:CDS 45.0%
!! TCATGGTGTTGTTGATGTGG+TGG - Chr7:24264112-24264131 MsG0780037361.01.T01:CDS 45.0%
!! TTTGGTGTCGGAATGCTTTG+TGG - Chr7:24264197-24264216 MsG0780037361.01.T01:CDS 45.0%
!!! AGTCGTGGATTTTTTGGGCT+CGG - Chr7:24264173-24264192 MsG0780037361.01.T01:CDS 45.0%
!!! GGATTTTTTGGGCTCGGATT+TGG - Chr7:24264179-24264198 MsG0780037361.01.T01:CDS 45.0%
!!! GGTGGTTGGTGTTTTAAGAG+AGG - Chr7:24264256-24264275 MsG0780037361.01.T01:CDS 45.0%
!!! GGTGTTTTAAGAGAGGAGGT+GGG - Chr7:24264263-24264282 MsG0780037361.01.T01:CDS 45.0%
!!! GGTTGGTGTTTTAAGAGAGG+AGG - Chr7:24264259-24264278 MsG0780037361.01.T01:CDS 45.0%
!!! TGGTGTTTTAAGAGAGGAGG+TGG - Chr7:24264262-24264281 MsG0780037361.01.T01:CDS 45.0%
GTGTCGGAATGCTTTGTGGA+CGG - Chr7:24264201-24264220 MsG0780037361.01.T01:CDS 50.0%
TGTCGGAATGCTTTGTGGAC+GGG - Chr7:24264202-24264221 MsG0780037361.01.T01:CDS 50.0%
! GGTGGTGATGATGTGTAGTG+TGG - Chr7:24264139-24264158 MsG0780037361.01.T01:CDS 50.0%
! GTGGTTTGGATGGATAGTCG+TGG - Chr7:24264158-24264177 MsG0780037361.01.T01:CDS 50.0%
!! TGTTGATGTGGTGGTGATGG+TGG - Chr7:24264121-24264140 MsG0780037361.01.T01:CDS 50.0%
!! TTTGGGCTCGGATTTGGTGT+CGG - Chr7:24264185-24264204 MsG0780037361.01.T01:CDS 50.0%
!!! AGAGGTTGGTTTTGTGGTGG+TGG - Chr7:24264238-24264257 MsG0780037361.01.T01:CDS 50.0%
!!! GGAAGAGGTTGGTTTTGTGG+TGG - Chr7:24264235-24264254 MsG0780037361.01.T01:CDS 50.0%
!!! GTTGGTTTTGTGGTGGTGGT+TGG - Chr7:24264242-24264261 MsG0780037361.01.T01:CDS 50.0%
AGAGGAGGTGGGTCCTTGTT+GGG - Chr7:24264274-24264293 MsG0780037361.01.T01:CDS 55.0%
GAGAGGAGGTGGGTCCTTGT+TGG - Chr7:24264273-24264292 MsG0780037361.01.T01:CDS 60.0%
GAGGAGGTGGGTCCTTGTTG+GGG - Chr7:24264275-24264294 MsG0780037361.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 24264032 24264310 24264032 ID=MsG0780037361.01;Name=MsG0780037361.01
Chr7 mRNA 24264032 24264310 24264032 ID=MsG0780037361.01.T01;Parent=MsG0780037361.01;Name=MsG0780037361.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|92
Chr7 exon 24264032 24264310 24264032 ID=MsG0780037361.01.T01:exon:3515;Parent=MsG0780037361.01.T01
Chr7 CDS 24264032 24264310 24264032 ID=MsG0780037361.01.T01:cds;Parent=MsG0780037361.01.T01
Gene Sequence

>MsG0780037361.01.T01

ATGGTGAGGTGTTTAGCTGGAGTAGTTCAGTCTTTATCGTTGGTGATGTGGGTATTCACCGTGACTGCTTCTTCGGAATGTCATGGTGTTGTTGATGTGGTGGTGATGGTGGTGATGATGTGTAGTGTGGTTTGGATGGATAGTCGTGGATTTTTTGGGCTCGGATTTGGTGTCGGAATGCTTTGTGGACGGGAGCTTTTTAAGGAAGAGGTTGGTTTTGTGGTGGTGGTTGGTGTTTTAAGAGAGGAGGTGGGTCCTTGTTGGGGTGTTTTCCTATAG
Protein sequence

>MsG0780037361.01.T01

MVRCLAGVVQSLSLVMWVFTVTASSECHGVVDVVVMVVMMCSVVWMDSRGFFGLGFGVGMLCGRELFKEEVGFVVVVGVLREEVGPCWGVFL*