AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037669.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037669.01.T01 MTR_2g010330 95.000 120 6 0 25 144 28 147 1.49e-66 213
MsG0780037669.01.T01 MTR_2g010300 80.342 117 23 0 27 143 26 142 2.92e-60 196
MsG0780037669.01.T01 MTR_2g010250 77.966 118 26 0 27 144 26 143 1.20e-59 195
MsG0780037669.01.T01 MTR_2g010320 89.320 103 11 0 42 144 41 143 2.35e-59 194
MsG0780037669.01.T01 MTR_2g010290 76.271 118 28 0 27 144 69 186 3.21e-57 189
MsG0780037669.01.T01 MTR_2g010280 80.769 104 20 0 40 143 39 142 6.71e-53 177
MsG0780037669.01.T01 MTR_2g010380 79.091 110 23 0 35 144 38 147 3.71e-49 167
MsG0780037669.01.T01 MTR_8g098565 70.000 100 28 1 46 143 45 144 3.14e-40 144
MsG0780037669.01.T01 MTR_5g034900 55.263 114 51 0 31 144 31 144 2.54e-39 141
MsG0780037669.01.T01 MTR_8g063280 52.239 134 57 2 31 164 30 156 3.29e-37 135
MsG0780037669.01.T01 MTR_8g063260 50.000 134 59 2 31 164 32 157 9.65e-35 129
MsG0780037669.01.T01 MTR_5g026520 48.305 118 57 2 31 145 29 145 3.76e-28 110
MsG0780037669.01.T01 MTR_1g492820 32.061 131 86 1 19 146 30 160 1.33e-23 98.2
MsG0780037669.01.T01 MTR_4g131830 36.885 122 71 2 28 146 30 148 3.85e-20 88.2
MsG0780037669.01.T01 MTR_5g070980 37.069 116 69 2 32 147 38 149 8.15e-15 70.9
MsG0780037669.01.T01 MTR_5g070710 34.109 129 81 2 19 147 24 148 4.11e-14 70.9
MsG0780037669.01.T01 MTR_5g070920 34.351 131 78 3 19 147 25 149 5.63e-14 70.5
MsG0780037669.01.T01 MTR_2g010530 33.333 120 77 2 25 144 28 144 1.25e-12 66.2
MsG0780037669.01.T01 MTR_5g070700 35.345 116 67 3 32 145 38 147 1.71e-12 65.9
MsG0780037669.01.T01 MTR_5g016410 31.538 130 85 2 20 149 21 146 2.45e-12 65.5
MsG0780037669.01.T01 MTR_8g091690 35.484 124 76 2 22 145 19 138 3.26e-12 65.1
MsG0780037669.01.T01 MTR_3g057900 35.593 118 73 2 27 144 32 146 3.86e-12 65.1
MsG0780037669.01.T01 MTR_5g095260 32.432 148 89 4 24 170 34 171 5.14e-12 64.7
MsG0780037669.01.T01 MTR_4g026070 34.513 113 67 3 22 132 20 127 5.50e-12 63.9
MsG0780037669.01.T01 MTR_2g065360 37.391 115 68 2 31 145 7 117 6.98e-12 64.3
MsG0780037669.01.T01 MTR_3g057970 34.454 119 75 2 27 145 29 144 9.69e-12 63.9
MsG0780037669.01.T01 MTR_5g016440 31.304 115 75 2 31 145 31 141 1.01e-11 63.5
MsG0780037669.01.T01 MTR_3g058250 34.454 119 75 2 27 145 31 146 2.61e-11 62.4
MsG0780037669.01.T01 MTR_8g104080 32.773 119 66 3 32 145 37 146 2.90e-11 61.6
MsG0780037669.01.T01 MTR_4g026050 29.921 127 82 3 22 146 20 141 3.19e-11 62.4
MsG0780037669.01.T01 MTR_5g470730 31.298 131 82 3 19 147 25 149 5.22e-11 61.6
MsG0780037669.01.T01 MTR_6g084770 32.258 124 76 3 24 145 30 147 5.31e-11 61.6
MsG0780037669.01.T01 MTR_3g058390 34.483 116 73 2 30 145 34 146 7.38e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037669.01.T01 AT3G03470 56.667 120 51 1 27 145 27 146 1.38e-43 152
MsG0780037669.01.T01 AT1G64950 61.404 114 44 0 31 144 32 145 2.10e-43 152
MsG0780037669.01.T01 AT2G12190 58.772 114 47 0 31 144 32 145 3.97e-42 149
MsG0780037669.01.T01 AT1G64940 57.895 114 48 0 31 144 33 146 1.26e-39 142
MsG0780037669.01.T01 AT5G61320 59.406 101 41 0 45 145 45 145 2.41e-36 133
MsG0780037669.01.T01 AT1G64930 51.304 115 55 1 31 145 32 145 1.02e-35 131
MsG0780037669.01.T01 AT1G64900 50.467 107 51 1 38 144 38 142 3.06e-32 122
MsG0780037669.01.T01 AT3G10560 34.356 163 99 3 31 191 46 202 1.01e-24 101
MsG0780037669.01.T01 AT5G04630 33.562 146 91 2 24 169 31 170 6.47e-23 96.3
MsG0780037669.01.T01 AT1G11600 31.469 143 92 2 7 146 11 150 5.19e-18 82.0
MsG0780037669.01.T01 AT3G10570 35.577 104 64 1 49 152 61 161 1.94e-17 80.5
MsG0780037669.01.T01 AT5G04660 35.556 90 58 0 57 146 67 156 2.68e-17 80.1
MsG0780037669.01.T01 AT4G37430 35.135 111 67 3 30 139 27 133 3.11e-11 62.4

Find 55 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGATGCTTGTGATAAT+TGG 0.278145 7:-30641553 None:intergenic
GGAGGGTTGCGGTGGTTTCT+TGG 0.288765 7:-30641510 None:intergenic
TTTCAGAGAATGATTGATCT+TGG 0.329211 7:-30641863 None:intergenic
ACTGATGTTGTGCTGGTTGC+TGG 0.330435 7:-30641773 None:intergenic
AGCACGGTCGGCGATGAAAA+TGG 0.334965 7:-30641671 None:intergenic
TTGCTAGTGGGAAGTGCTTT+GGG 0.346056 7:-30641742 None:intergenic
ATCAATCATTCTCTGAAATT+CGG 0.354217 7:+30641868 MsG0780037669.01.T01:CDS
TTTGCTAGTGGGAAGTGCTT+TGG 0.365166 7:-30641743 None:intergenic
TGGTTTCTTGGTGGTGGAAA+TGG 0.369510 7:-30641498 None:intergenic
GGGTTGCGGTGGTTTCTTGG+TGG 0.376501 7:-30641507 None:intergenic
TATGTGGTGGACCGGGAGGG+AGG 0.380923 7:-30641528 None:intergenic
CCTATCATCAATCTTAATAT+CGG 0.409593 7:+30641637 MsG0780037669.01.T01:CDS
ACCGGGAGGGAGGGTTGCGG+TGG 0.422549 7:-30641518 None:intergenic
ATTGATGATAGGACCATGTT+TGG 0.424665 7:-30641626 None:intergenic
TTTGAGAAAAGGTTCGAGTT+CGG 0.426522 7:-30641593 None:intergenic
TTTCATCGCCGACCGTGCTC+TGG 0.433250 7:+30641675 MsG0780037669.01.T01:CDS
TGGACCGGGAGGGAGGGTTG+CGG 0.457490 7:-30641521 None:intergenic
TTGCGGTGGTTTCTTGGTGG+TGG 0.466572 7:-30641504 None:intergenic
CCGATATTAAGATTGATGAT+AGG 0.467922 7:-30641637 None:intergenic
GGAATCTGCGGATTCTGTTA+CGG 0.475473 7:+30641935 MsG0780037669.01.T01:CDS
AGAGAATGATTGATCTTGGA+TGG 0.497905 7:-30641859 None:intergenic
CATTCTCTGAAATTCGGAAG+TGG 0.504370 7:+30641874 MsG0780037669.01.T01:CDS
ATTCTCTGAAATTCGGAAGT+GGG 0.520342 7:+30641875 MsG0780037669.01.T01:CDS
TGGTGGTGGAAATGGATGAA+AGG 0.521476 7:-30641490 None:intergenic
GGTGGTGGAAATGGATGAAA+GGG 0.535385 7:-30641489 None:intergenic
ACCACCGCAACCCTCCCTCC+CGG 0.544974 7:+30641517 MsG0780037669.01.T01:CDS
ATGTGGTGGACCGGGAGGGA+GGG 0.552886 7:-30641527 None:intergenic
AATTGGTATATGTGGTGGAC+CGG 0.554797 7:-30641536 None:intergenic
ATTGGTATATGTGGTGGACC+GGG 0.564040 7:-30641535 None:intergenic
CTTGTGATAATTGGTATATG+TGG 0.565304 7:-30641544 None:intergenic
GAGCGTGGTGGGCCAGAGCA+CGG 0.572102 7:-30641687 None:intergenic
AAGCGGAACTGATGTTGTGC+TGG 0.572282 7:-30641780 None:intergenic
TACAAAGGACACTTCTGTTG+AGG 0.575033 7:+30642054 MsG0780037669.01.T01:CDS
CAACCGTCTCAAAACTGCAT+CGG 0.585559 7:+30641914 MsG0780037669.01.T01:CDS
GTATATGTGGTGGACCGGGA+GGG 0.586526 7:-30641531 None:intergenic
CTTGGATGGATGAAGCAACT+CGG 0.587682 7:-30641845 None:intergenic
AAAACTCTTCATGCCAAACA+TGG 0.588367 7:+30641613 MsG0780037669.01.T01:CDS
CGACGGAGAGTACGCCATGT+TGG 0.597150 7:-30641811 None:intergenic
CAAAACTGCATCGGAATCTG+CGG 0.601293 7:+30641923 MsG0780037669.01.T01:CDS
GTGGTGGGCCAGAGCACGGT+CGG 0.601407 7:-30641683 None:intergenic
GTGATAATTGGTATATGTGG+TGG 0.602245 7:-30641541 None:intergenic
GGTATATGTGGTGGACCGGG+AGG 0.605113 7:-30641532 None:intergenic
AACATAGCATAATGAAAGTG+AGG 0.611614 7:-30641963 None:intergenic
CAATTATCACAAGCATCCAA+TGG 0.612431 7:+30641554 MsG0780037669.01.T01:CDS
GATGAAGCAACTCGGAGGCG+AGG 0.630968 7:-30641837 None:intergenic
GCTGGACATGAGTTTGCTAG+TGG 0.631034 7:-30641755 None:intergenic
CGGTCGGCGATGAAAATGGA+AGG 0.633983 7:-30641667 None:intergenic
TTTGGGACGATCAGAGAAGA+CGG 0.637298 7:-30641725 None:intergenic
CTGGACATGAGTTTGCTAGT+GGG 0.638924 7:-30641754 None:intergenic
GTGATATTGAGCGTGTACAA+AGG 0.640955 7:+30642039 MsG0780037669.01.T01:CDS
ACTCGGAGGCGAGGTTACGA+CGG 0.654878 7:-30641828 None:intergenic
GGTGGAAATGGATGAAAGGG+TGG 0.655108 7:-30641486 None:intergenic
ATGAAAGGGTGGCTCTGATG+AGG 0.671580 7:-30641475 None:intergenic
GGATGGATGAAGCAACTCGG+AGG 0.712810 7:-30641842 None:intergenic
GGGACGATCAGAGAAGACGG+AGG 0.722328 7:-30641722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTGTTTTTATGTGTTTT+GGG + Chr7:30641991-30642010 MsG0780037669.01.T01:CDS 20.0%
!!! TTACTTGTTTTTATGTGTTT+TGG + Chr7:30641990-30642009 MsG0780037669.01.T01:CDS 20.0%
! CCTATCATCAATCTTAATAT+CGG + Chr7:30641637-30641656 MsG0780037669.01.T01:CDS 25.0%
!!! ACTTGTTTTTATGTGTTTTG+GGG + Chr7:30641992-30642011 MsG0780037669.01.T01:CDS 25.0%
!!! ATCAATCATTCTCTGAAATT+CGG + Chr7:30641868-30641887 MsG0780037669.01.T01:CDS 25.0%
AACATAGCATAATGAAAGTG+AGG - Chr7:30641966-30641985 None:intergenic 30.0%
CTTGTGATAATTGGTATATG+TGG - Chr7:30641547-30641566 None:intergenic 30.0%
TTTCAGAGAATGATTGATCT+TGG - Chr7:30641866-30641885 None:intergenic 30.0%
! CCGATATTAAGATTGATGAT+AGG - Chr7:30641640-30641659 None:intergenic 30.0%
AAAACTCTTCATGCCAAACA+TGG + Chr7:30641613-30641632 MsG0780037669.01.T01:CDS 35.0%
AGAGAATGATTGATCTTGGA+TGG - Chr7:30641862-30641881 None:intergenic 35.0%
CAATTATCACAAGCATCCAA+TGG + Chr7:30641554-30641573 MsG0780037669.01.T01:CDS 35.0%
CATTGGATGCTTGTGATAAT+TGG - Chr7:30641556-30641575 None:intergenic 35.0%
GTGATAATTGGTATATGTGG+TGG - Chr7:30641544-30641563 None:intergenic 35.0%
! ATTGATGATAGGACCATGTT+TGG - Chr7:30641629-30641648 None:intergenic 35.0%
! TTTGAGAAAAGGTTCGAGTT+CGG - Chr7:30641596-30641615 None:intergenic 35.0%
!! ATTCTCTGAAATTCGGAAGT+GGG + Chr7:30641875-30641894 MsG0780037669.01.T01:CDS 35.0%
!!! AACATCAGTTCCGCTTTTTA+TGG + Chr7:30641787-30641806 MsG0780037669.01.T01:CDS 35.0%
!!! GCATGAAGAGTTTTGAGAAA+AGG - Chr7:30641607-30641626 None:intergenic 35.0%
CCATGTTGGTCCATAAAAAG+CGG - Chr7:30641800-30641819 None:intergenic 40.0%
GTGATATTGAGCGTGTACAA+AGG + Chr7:30642039-30642058 MsG0780037669.01.T01:CDS 40.0%
!! AATTGGTATATGTGGTGGAC+CGG - Chr7:30641539-30641558 None:intergenic 40.0%
!! CATTCTCTGAAATTCGGAAG+TGG + Chr7:30641874-30641893 MsG0780037669.01.T01:CDS 40.0%
!!! ATTCCGATGCAGTTTTGAGA+CGG - Chr7:30641920-30641939 None:intergenic 40.0%
CAAAACTGCATCGGAATCTG+CGG + Chr7:30641923-30641942 MsG0780037669.01.T01:CDS 45.0%
CAACCGTCTCAAAACTGCAT+CGG + Chr7:30641914-30641933 MsG0780037669.01.T01:CDS 45.0%
CTTGGATGGATGAAGCAACT+CGG - Chr7:30641848-30641867 None:intergenic 45.0%
GGAATCTGCGGATTCTGTTA+CGG + Chr7:30641935-30641954 MsG0780037669.01.T01:CDS 45.0%
! CGAATGATTTTCGGAGCCAT+TGG - Chr7:30641573-30641592 None:intergenic 45.0%
! CGAGTTCGGCGAATGATTTT+CGG - Chr7:30641582-30641601 None:intergenic 45.0%
! TTTGGGACGATCAGAGAAGA+CGG - Chr7:30641728-30641747 None:intergenic 45.0%
!! ATTGGTATATGTGGTGGACC+GGG - Chr7:30641538-30641557 None:intergenic 45.0%
!! CTGGACATGAGTTTGCTAGT+GGG - Chr7:30641757-30641776 None:intergenic 45.0%
!! GGTGGTGGAAATGGATGAAA+GGG - Chr7:30641492-30641511 None:intergenic 45.0%
!! TGGTGGTGGAAATGGATGAA+AGG - Chr7:30641493-30641512 None:intergenic 45.0%
!! TGGTTTCTTGGTGGTGGAAA+TGG - Chr7:30641501-30641520 None:intergenic 45.0%
!! TTGCTAGTGGGAAGTGCTTT+GGG - Chr7:30641745-30641764 None:intergenic 45.0%
!! TTTGCTAGTGGGAAGTGCTT+TGG - Chr7:30641746-30641765 None:intergenic 45.0%
!!! CCGCTTTTTATGGACCAACA+TGG + Chr7:30641797-30641816 MsG0780037669.01.T01:CDS 45.0%
AAGCGGAACTGATGTTGTGC+TGG - Chr7:30641783-30641802 None:intergenic 50.0%
GGTGGAAATGGATGAAAGGG+TGG - Chr7:30641489-30641508 None:intergenic 50.0%
!! ACTGATGTTGTGCTGGTTGC+TGG - Chr7:30641776-30641795 None:intergenic 50.0%
!! ATGAAAGGGTGGCTCTGATG+AGG - Chr7:30641478-30641497 None:intergenic 50.0%
!! GCTGGACATGAGTTTGCTAG+TGG - Chr7:30641758-30641777 None:intergenic 50.0%
!! GTTTTGAACGAGAGCGTGGT+GGG - Chr7:30641701-30641720 None:intergenic 50.0%
!!! AGGAGTTTTGAACGAGAGCG+TGG - Chr7:30641705-30641724 None:intergenic 50.0%
!!! AGTTTTGAACGAGAGCGTGG+TGG - Chr7:30641702-30641721 None:intergenic 50.0%
AGCACGGTCGGCGATGAAAA+TGG - Chr7:30641674-30641693 None:intergenic 55.0%
CGGTCGGCGATGAAAATGGA+AGG - Chr7:30641670-30641689 None:intergenic 55.0%
GGATGGATGAAGCAACTCGG+AGG - Chr7:30641845-30641864 None:intergenic 55.0%
TTGCGGTGGTTTCTTGGTGG+TGG - Chr7:30641507-30641526 None:intergenic 55.0%
!! GTATATGTGGTGGACCGGGA+GGG - Chr7:30641534-30641553 None:intergenic 55.0%
ACTCGGAGGCGAGGTTACGA+CGG - Chr7:30641831-30641850 None:intergenic 60.0%
GATGAAGCAACTCGGAGGCG+AGG - Chr7:30641840-30641859 None:intergenic 60.0%
GGAGGGTTGCGGTGGTTTCT+TGG - Chr7:30641513-30641532 None:intergenic 60.0%
GGGACGATCAGAGAAGACGG+AGG - Chr7:30641725-30641744 None:intergenic 60.0%
GGGTTGCGGTGGTTTCTTGG+TGG - Chr7:30641510-30641529 None:intergenic 60.0%
TTTCATCGCCGACCGTGCTC+TGG + Chr7:30641675-30641694 MsG0780037669.01.T01:CDS 60.0%
! CGACGGAGAGTACGCCATGT+TGG - Chr7:30641814-30641833 None:intergenic 60.0%
!! GGTATATGTGGTGGACCGGG+AGG - Chr7:30641535-30641554 None:intergenic 60.0%
!! ATGTGGTGGACCGGGAGGGA+GGG - Chr7:30641530-30641549 None:intergenic 65.0%
!! TATGTGGTGGACCGGGAGGG+AGG - Chr7:30641531-30641550 None:intergenic 65.0%
ACCACCGCAACCCTCCCTCC+CGG + Chr7:30641517-30641536 MsG0780037669.01.T01:CDS 70.0%
TGGACCGGGAGGGAGGGTTG+CGG - Chr7:30641524-30641543 None:intergenic 70.0%
!! GAGCGTGGTGGGCCAGAGCA+CGG - Chr7:30641690-30641709 None:intergenic 70.0%
!! GTGGTGGGCCAGAGCACGGT+CGG - Chr7:30641686-30641705 None:intergenic 70.0%
ACCGGGAGGGAGGGTTGCGG+TGG - Chr7:30641521-30641540 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr7 gene 30641439 30642074 30641439 ID=MsG0780037669.01;Name=MsG0780037669.01
Chr7 mRNA 30641439 30642074 30641439 ID=MsG0780037669.01.T01;Parent=MsG0780037669.01;Name=MsG0780037669.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|211
Chr7 exon 30641439 30642074 30641439 ID=MsG0780037669.01.T01:exon:1718;Parent=MsG0780037669.01.T01
Chr7 CDS 30641439 30642074 30641439 ID=MsG0780037669.01.T01:cds;Parent=MsG0780037669.01.T01
Gene Sequence

>MsG0780037669.01.T01

ATGGTTCATTGCACTTGTTTCTCTCTGCATCATCTTCCTCATCAGAGCCACCCTTTCATCCATTTCCACCACCAAGAAACCACCGCAACCCTCCCTCCCGGTCCACCACATATACCAATTATCACAAGCATCCAATGGCTCCGAAAATCATTCGCCGAACTCGAACCTTTTCTCAAAACTCTTCATGCCAAACATGGTCCTATCATCAATCTTAATATCGGTTCTCGACCTTCCATTTTCATCGCCGACCGTGCTCTGGCCCACCACGCTCTCGTTCAAAACTCCTCCGTCTTCTCTGATCGTCCCAAAGCACTTCCCACTAGCAAACTCATGTCCAGCAACCAGCACAACATCAGTTCCGCTTTTTATGGACCAACATGGCGTACTCTCCGTCGTAACCTCGCCTCCGAGTTGCTTCATCCATCCAAGATCAATCATTCTCTGAAATTCGGAAGTGGGTTCTGCACACTCTCATCAACCGTCTCAAAACTGCATCGGAATCTGCGGATTCTGTTACGGTTGTTCCTCACTTTCATTATGCTATGTTTTGTTTACTTGTTTTTATGTGTTTTGGGGAAAGAGTCAACGATGAGAAAATTAGTGATATTGAGCGTGTACAAAGGACACTTCTGTTGA
Protein sequence

>MsG0780037669.01.T01

MVHCTCFSLHHLPHQSHPFIHFHHQETTATLPPGPPHIPIITSIQWLRKSFAELEPFLKTLHAKHGPIINLNIGSRPSIFIADRALAHHALVQNSSVFSDRPKALPTSKLMSSNQHNISSAFYGPTWRTLRRNLASELLHPSKINHSLKFGSGFCTLSSTVSKLHRNLRILLRLFLTFIMLCFVYLFLCVLGKESTMRKLVILSVYKGHFC*