Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037762.01.T01 | RDX71746.1 | 70.199 | 151 | 6 | 1 | 12 | 123 | 89 | 239 | 2.36E-60 | 198 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037762.01.T01 | O23710 | 66.897 | 145 | 9 | 1 | 11 | 116 | 48 | 192 | 3.49E-59 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037762.01.T01 | I1JJI5 | 71.831 | 142 | 1 | 1 | 11 | 113 | 47 | 188 | 5.61e-61 | 195 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011059.01 | MsG0780037762.01 | 0.824624 | 7.295866e-54 | 6.498137e-51 |
| MsG0480021776.01 | MsG0780037762.01 | 0.807131 | 5.872784e-50 | 3.244869e-47 |
| MsG0280007193.01 | MsG0780037762.01 | 0.804288 | 2.321399e-49 | 1.192303e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037762.01.T01 | MTR_7g037830 | 66.452 | 155 | 13 | 1 | 11 | 126 | 47 | 201 | 1.86e-62 | 192 |
| MsG0780037762.01.T01 | MTR_7g032930 | 66.452 | 155 | 13 | 1 | 11 | 126 | 47 | 201 | 2.34e-62 | 191 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037762.01.T01 | AT3G27430 | 64.706 | 153 | 15 | 1 | 3 | 116 | 34 | 186 | 1.03e-60 | 187 |
| MsG0780037762.01.T01 | AT5G40580 | 64.706 | 153 | 15 | 1 | 3 | 116 | 34 | 186 | 1.05e-60 | 187 |
| MsG0780037762.01.T01 | AT5G40580 | 64.706 | 153 | 15 | 1 | 3 | 116 | 34 | 186 | 1.05e-60 | 187 |
| MsG0780037762.01.T01 | AT3G27430 | 66.897 | 145 | 9 | 1 | 11 | 116 | 48 | 192 | 3.44e-60 | 186 |
| MsG0780037762.01.T01 | AT3G27430 | 66.897 | 145 | 9 | 1 | 11 | 116 | 48 | 192 | 3.56e-60 | 186 |
| MsG0780037762.01.T01 | AT5G40580 | 66.897 | 145 | 9 | 1 | 11 | 116 | 48 | 192 | 4.17e-60 | 186 |
| MsG0780037762.01.T01 | AT5G40580 | 66.897 | 145 | 9 | 1 | 11 | 116 | 48 | 192 | 4.17e-60 | 186 |
Find 28 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCAGCTGCGTTAGTTCT+TGG | 0.243363 | 7:-32858263 | MsG0780037762.01.T01:intron |
| TTGTGAGAAAATTCATTATA+TGG | 0.247284 | 7:-32858675 | MsG0780037762.01.T01:CDS |
| TCTCAGGATGGTATCATTCT+TGG | 0.319515 | 7:-32858743 | MsG0780037762.01.T01:CDS |
| GAGTGTAAGTAAGCTTATTA+AGG | 0.321438 | 7:-32858001 | MsG0780037762.01.T01:CDS |
| GCTGATACGGAGGCTGTAAC+AGG | 0.392598 | 7:-32858617 | MsG0780037762.01.T01:intron |
| CTTGGTGGGGTTGACATCAC+TGG | 0.394539 | 7:-32858245 | MsG0780037762.01.T01:CDS |
| ACCCTTCCATTTGCTACAAT+GGG | 0.405524 | 7:-32858078 | MsG0780037762.01.T01:CDS |
| CCATTTGCTACAATGGGATC+AGG | 0.409457 | 7:-32858072 | MsG0780037762.01.T01:CDS |
| CAGCTGCGTTAGTTCTTGGT+GGG | 0.418941 | 7:-32858259 | MsG0780037762.01.T01:CDS |
| CACCCTTCCATTTGCTACAA+TGG | 0.435771 | 7:-32858079 | MsG0780037762.01.T01:CDS |
| CCTGATCCCATTGTAGCAAA+TGG | 0.436802 | 7:+32858072 | None:intergenic |
| AACATATATTGCTGTGGAGC+AGG | 0.450701 | 7:-32858647 | MsG0780037762.01.T01:CDS |
| AGCAGGCACTGCTGCTGATA+CGG | 0.456686 | 7:-32858630 | MsG0780037762.01.T01:CDS |
| TCCTTCAGTTGCTCTGGTAT+CGG | 0.479498 | 7:+32858718 | None:intergenic |
| TCAGCTGCGTTAGTTCTTGG+TGG | 0.496481 | 7:-32858260 | MsG0780037762.01.T01:intron |
| AATGGGTCCTTCAGTTGCTC+TGG | 0.509012 | 7:+32858712 | None:intergenic |
| GCTCCACAGCAATATATGTT+GGG | 0.513319 | 7:+32858650 | None:intergenic |
| AGGCACTGCTGCTGATACGG+AGG | 0.552659 | 7:-32858627 | MsG0780037762.01.T01:CDS |
| TGCTCCACAGCAATATATGT+TGG | 0.555044 | 7:+32858649 | None:intergenic |
| AAGGGTGTCAGTTGACCCAT+GGG | 0.555536 | 7:+32858095 | None:intergenic |
| ATCCCATTGTAGCAAATGGA+AGG | 0.564159 | 7:+32858076 | None:intergenic |
| GAAGGGTGTCAGTTGACCCA+TGG | 0.564216 | 7:+32858094 | None:intergenic |
| AGCTGCGTTAGTTCTTGGTG+GGG | 0.574763 | 7:-32858258 | MsG0780037762.01.T01:CDS |
| GAACTAACAGTGTGTAGATG+TGG | 0.589215 | 7:+32858221 | None:intergenic |
| TCCCATTGTAGCAAATGGAA+GGG | 0.632486 | 7:+32858077 | None:intergenic |
| GCCGATACCAGAGCAACTGA+AGG | 0.641387 | 7:-32858719 | MsG0780037762.01.T01:CDS |
| GCACCCAACATATATTGCTG+TGG | 0.661044 | 7:-32858653 | MsG0780037762.01.T01:CDS |
| AGGGTGTCAGTTGACCCATG+GGG | 0.760479 | 7:+32858096 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTTATAAAATGTTACATA+TGG | - | Chr7:32858189-32858208 | MsG0780037762.01.T01:intron | 15.0% |
| !!! | CTAAAGTTTTTTTTTTTGTT+CGG | - | Chr7:32858565-32858584 | MsG0780037762.01.T01:intron | 15.0% |
| !! | TAACAAACATATCATTTCAA+TGG | + | Chr7:32858252-32858271 | None:intergenic | 20.0% |
| !! | TTGTGAGAAAATTCATTATA+TGG | - | Chr7:32858064-32858083 | MsG0780037762.01.T01:CDS | 20.0% |
| !!! | TTTGTTATTTATTGTAGACA+TGG | - | Chr7:32858265-32858284 | MsG0780037762.01.T01:intron | 20.0% |
| ! | AAAAATAAATTGTCTCAGGA+TGG | - | Chr7:32857984-32858003 | MsG0780037762.01.T01:CDS | 25.0% |
| ! | TATAAAATGTTACATATGGC+TGG | - | Chr7:32858193-32858212 | MsG0780037762.01.T01:intron | 25.0% |
| ! | TTCCAAAAATAAATTGTCTC+AGG | - | Chr7:32857980-32857999 | MsG0780037762.01.T01:CDS | 25.0% |
| !!! | ATCCTGAGACAATTTATTTT+TGG | + | Chr7:32857985-32858004 | None:intergenic | 25.0% |
| !!! | GTTTTTTTTTTTGTTCGGAA+TGG | - | Chr7:32858570-32858589 | MsG0780037762.01.T01:intron | 25.0% |
| !!! | TTCTGAAACTAAACTTGAAA+AGG | + | Chr7:32858373-32858392 | None:intergenic | 25.0% |
| AGGAGCAAAATAACTTGAAA+CGG | + | Chr7:32858403-32858422 | None:intergenic | 30.0% | |
| ATTTGAAGAGTTAGAATCAG+TGG | + | Chr7:32858434-32858453 | None:intergenic | 30.0% | |
| GAGTGTAAGTAAGCTTATTA+AGG | - | Chr7:32858738-32858757 | MsG0780037762.01.T01:CDS | 30.0% | |
| ! | ACAATTTTTATCTGCGACAA+TGG | + | Chr7:32858048-32858067 | None:intergenic | 30.0% |
| ! | CAATTTTTATCTGCGACAAT+GGG | + | Chr7:32858047-32858066 | None:intergenic | 30.0% |
| CAAATTATGTGCAGTTACCA+AGG | - | Chr7:32858449-32858468 | MsG0780037762.01.T01:intron | 35.0% | |
| CTGTAACAACTCTTGATTCT+CGG | + | Chr7:32858328-32858347 | None:intergenic | 35.0% | |
| GCAAAATAACTTGAAACGGA+TGG | + | Chr7:32858399-32858418 | None:intergenic | 35.0% | |
| GTTACATATGGCTGGTATTT+AGG | - | Chr7:32858201-32858220 | MsG0780037762.01.T01:intron | 35.0% | |
| TAGAATCAGTGGTTGTTACA+AGG | + | Chr7:32858423-32858442 | None:intergenic | 35.0% | |
| AACATATATTGCTGTGGAGC+AGG | - | Chr7:32858092-32858111 | MsG0780037762.01.T01:CDS | 40.0% | |
| ATCCCATTGTAGCAAATGGA+AGG | + | Chr7:32858666-32858685 | None:intergenic | 40.0% | |
| CAATTACATCGGTACCATAC+TGG | - | Chr7:32858302-32858321 | MsG0780037762.01.T01:intron | 40.0% | |
| GAACTAACAGTGTGTAGATG+TGG | + | Chr7:32858521-32858540 | None:intergenic | 40.0% | |
| GCTCCACAGCAATATATGTT+GGG | + | Chr7:32858092-32858111 | None:intergenic | 40.0% | |
| TCCCATTGTAGCAAATGGAA+GGG | + | Chr7:32858665-32858684 | None:intergenic | 40.0% | |
| TCTCAGGATGGTATCATTCT+TGG | - | Chr7:32857996-32858015 | MsG0780037762.01.T01:CDS | 40.0% | |
| TGCTCCACAGCAATATATGT+TGG | + | Chr7:32858093-32858112 | None:intergenic | 40.0% | |
| ! | ACCCTTCCATTTGCTACAAT+GGG | - | Chr7:32858661-32858680 | MsG0780037762.01.T01:CDS | 40.0% |
| ! | TTTTGCAGATCTATCCCCAT+GGG | - | Chr7:32858629-32858648 | MsG0780037762.01.T01:CDS | 40.0% |
| CCTGATCCCATTGTAGCAAA+TGG | + | Chr7:32858670-32858689 | None:intergenic | 45.0% | |
| GCACCCAACATATATTGCTG+TGG | - | Chr7:32858086-32858105 | MsG0780037762.01.T01:CDS | 45.0% | |
| TCCTTCAGTTGCTCTGGTAT+CGG | + | Chr7:32858024-32858043 | None:intergenic | 45.0% | |
| ! | CACCCTTCCATTTGCTACAA+TGG | - | Chr7:32858660-32858679 | MsG0780037762.01.T01:CDS | 45.0% |
| ! | CCATTTGCTACAATGGGATC+AGG | - | Chr7:32858667-32858686 | MsG0780037762.01.T01:CDS | 45.0% |
| ! | GTACCGATGTAATTGCAGCT+GGG | + | Chr7:32858297-32858316 | None:intergenic | 45.0% |
| ! | GTTTTGCAGATCTATCCCCA+TGG | - | Chr7:32858628-32858647 | MsG0780037762.01.T01:CDS | 45.0% |
| AATGGGTCCTTCAGTTGCTC+TGG | + | Chr7:32858030-32858049 | None:intergenic | 50.0% | |
| CTCTTGATTCTCGGCCAGTA+TGG | + | Chr7:32858319-32858338 | None:intergenic | 50.0% | |
| GCTCCCAGCTGCAATTACAT+CGG | - | Chr7:32858291-32858310 | MsG0780037762.01.T01:intron | 50.0% | |
| ! | AGCTGCGTTAGTTCTTGGTG+GGG | - | Chr7:32858481-32858500 | MsG0780037762.01.T01:intron | 50.0% |
| ! | CAGCTGCGTTAGTTCTTGGT+GGG | - | Chr7:32858480-32858499 | MsG0780037762.01.T01:intron | 50.0% |
| ! | GTCTCAGCTGCGTTAGTTCT+TGG | - | Chr7:32858476-32858495 | MsG0780037762.01.T01:intron | 50.0% |
| ! | TCAGCTGCGTTAGTTCTTGG+TGG | - | Chr7:32858479-32858498 | MsG0780037762.01.T01:intron | 50.0% |
| !! | AAGGGTGTCAGTTGACCCAT+GGG | + | Chr7:32858647-32858666 | None:intergenic | 50.0% |
| !! | GGTACCGATGTAATTGCAGC+TGG | + | Chr7:32858298-32858317 | None:intergenic | 50.0% |
| ACGCAGCTGAGACATGACCT+TGG | + | Chr7:32858469-32858488 | None:intergenic | 55.0% | |
| CTTGGTGGGGTTGACATCAC+TGG | - | Chr7:32858494-32858513 | MsG0780037762.01.T01:intron | 55.0% | |
| GCCGATACCAGAGCAACTGA+AGG | - | Chr7:32858020-32858039 | MsG0780037762.01.T01:CDS | 55.0% | |
| GCTGATACGGAGGCTGTAAC+AGG | - | Chr7:32858122-32858141 | MsG0780037762.01.T01:intron | 55.0% | |
| ! | AGCAGGCACTGCTGCTGATA+CGG | - | Chr7:32858109-32858128 | MsG0780037762.01.T01:intron | 55.0% |
| ! | AGGGTGTCAGTTGACCCATG+GGG | + | Chr7:32858646-32858665 | None:intergenic | 55.0% |
| !! | GAAGGGTGTCAGTTGACCCA+TGG | + | Chr7:32858648-32858667 | None:intergenic | 55.0% |
| ! | AGGCACTGCTGCTGATACGG+AGG | - | Chr7:32858112-32858131 | MsG0780037762.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 32857969 | 32858792 | 32857969 | ID=MsG0780037762.01;Name=MsG0780037762.01 |
| Chr7 | mRNA | 32857969 | 32858792 | 32857969 | ID=MsG0780037762.01.T01;Parent=MsG0780037762.01;Name=MsG0780037762.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0.5|0|1|0.5|0.66|3|0|126 |
| Chr7 | exon | 32858618 | 32858792 | 32858618 | ID=MsG0780037762.01.T01:exon:950;Parent=MsG0780037762.01.T01 |
| Chr7 | exon | 32858229 | 32858278 | 32858229 | ID=MsG0780037762.01.T01:exon:951;Parent=MsG0780037762.01.T01 |
| Chr7 | exon | 32857969 | 32858124 | 32857969 | ID=MsG0780037762.01.T01:exon:952;Parent=MsG0780037762.01.T01 |
| Chr7 | CDS | 32858618 | 32858792 | 32858618 | ID=MsG0780037762.01.T01:cds;Parent=MsG0780037762.01.T01 |
| Chr7 | CDS | 32858229 | 32858278 | 32858229 | ID=MsG0780037762.01.T01:cds;Parent=MsG0780037762.01.T01 |
| Chr7 | CDS | 32857969 | 32858124 | 32857969 | ID=MsG0780037762.01.T01:cds;Parent=MsG0780037762.01.T01 |
| Gene Sequence |
| Protein sequence |
