Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038613.01.T02 | AES79176.1 | 96.581 | 117 | 2 | 2 | 1 | 115 | 1 | 117 | 3.13E-72 | 221 |
| MsG0780038613.01.T01 | AES79176.1 | 88.889 | 117 | 1 | 2 | 1 | 105 | 1 | 117 | 6.40E-64 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038613.01.T01 | A1EC31 | 36.364 | 132 | 57 | 3 | 1 | 105 | 1 | 132 | 1.45E-12 | 62.4 |
| MsG0780038613.01.T02 | A1EC31 | 38.636 | 132 | 64 | 3 | 1 | 115 | 1 | 132 | 5.06E-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038613.01.T01 | G7L1C0 | 88.889 | 117 | 1 | 2 | 1 | 105 | 1 | 117 | 3.06e-64 | 199 |
| MsG0780038613.01.T02 | G7L1C0 | 96.581 | 117 | 2 | 2 | 1 | 115 | 1 | 117 | 1.50e-72 | 221 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014881.01 | MsG0780038613.01 | 0.826549 | 2.553126e-54 | 2.401814e-51 |
| MsG0580028478.01 | MsG0780038613.01 | 0.816395 | 5.653589e-52 | 4.001334e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038613.01.T02 | MTR_7g058790 | 96.581 | 117 | 2 | 2 | 1 | 115 | 1 | 117 | 3.79e-76 | 221 |
| MsG0780038613.01.T01 | MTR_7g058790 | 88.889 | 117 | 1 | 2 | 1 | 105 | 1 | 117 | 7.75e-68 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATACTTACCGATTTGAAAT+AGG | 0.185862 | 7:+49949894 | None:intergenic |
| ATTGAACCCTTGTGGGCTAT+TGG | 0.268531 | 7:-49950218 | MsG0780038613.01.T02:CDS |
| TTGAACCCTTGTGGGCTATT+GGG | 0.273811 | 7:-49950217 | MsG0780038613.01.T02:CDS |
| ATACTTACCGATTTGAAATA+GGG | 0.289778 | 7:+49949895 | None:intergenic |
| TTTGTAGGGTTTGTGGTTGT+TGG | 0.301736 | 7:+49950065 | None:intergenic |
| GAGATAGGTTGTGAGGTATT+AGG | 0.304793 | 7:+49950161 | None:intergenic |
| GATGGAGCTGGAAAAGAATT+TGG | 0.313620 | 7:-49949951 | MsG0780038613.01.T02:CDS |
| GTCATAGAAGAAGGGTTTGT+AGG | 0.341733 | 7:+49950050 | None:intergenic |
| TACCGATTTGAAATAGGGTT+TGG | 0.375351 | 7:+49949900 | None:intergenic |
| TGATTTCGATTCAGTTGATT+TGG | 0.380301 | 7:+49950022 | None:intergenic |
| TGAACCCTTGTGGGCTATTG+GGG | 0.382939 | 7:-49950216 | MsG0780038613.01.T02:CDS |
| AGAAGGGTTTGTAGGGTTTG+TGG | 0.386629 | 7:+49950058 | None:intergenic |
| TCATAGAAGAAGGGTTTGTA+GGG | 0.438501 | 7:+49950051 | None:intergenic |
| TTGGTAGAAGTCATAGAAGA+AGG | 0.440916 | 7:+49950041 | None:intergenic |
| TGGTAGAAGTCATAGAAGAA+GGG | 0.488580 | 7:+49950042 | None:intergenic |
| GTCCAAACCCTATTTCAAAT+CGG | 0.494361 | 7:-49949902 | MsG0780038613.01.T02:CDS |
| GAAATAGGGTTTGGACCACT+TGG | 0.510460 | 7:+49949909 | None:intergenic |
| GATGGATATTGAACCCTTGT+GGG | 0.522506 | 7:-49950225 | MsG0780038613.01.T02:CDS |
| GCACCCACACAAAACACAAA+AGG | 0.525409 | 7:-49949973 | MsG0780038613.01.T02:CDS |
| GAACCCTTGTGGGCTATTGG+GGG | 0.526035 | 7:-49950215 | MsG0780038613.01.T02:CDS |
| ATAAATTGATGATGAGGAGA+AGG | 0.536526 | 7:+49950096 | None:intergenic |
| TGATGGATATTGAACCCTTG+TGG | 0.557876 | 7:-49950226 | MsG0780038613.01.T02:CDS |
| AAAACACAAAAGGATGGAGC+TGG | 0.564479 | 7:-49949963 | MsG0780038613.01.T02:CDS |
| CCACACAAAACACAAAAGGA+TGG | 0.594234 | 7:-49949969 | MsG0780038613.01.T02:CDS |
| AACCCTTGTGGGCTATTGGG+GGG | 0.599281 | 7:-49950214 | MsG0780038613.01.T02:CDS |
| GCTGAGATAAATTGATGATG+AGG | 0.620192 | 7:+49950090 | None:intergenic |
| AACTGAATCGAAATCAACCT+CGG | 0.639526 | 7:-49950015 | MsG0780038613.01.T02:CDS |
| AGGAAGTGAAAGAAATTGAG+TGG | 0.671864 | 7:+49950134 | None:intergenic |
| GTTTGTCGTAGATGTCGCCG+AGG | 0.695081 | 7:+49949998 | None:intergenic |
| GATGCTCATGAAGTTCCAAG+TGG | 0.717564 | 7:-49949924 | MsG0780038613.01.T02:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTTTTCTTTCAATTTGCA+TGG | - | Chr7:49949934-49949953 | MsG0780038613.01.T02:CDS | 25.0% |
| TGATTTCGATTCAGTTGATT+TGG | + | Chr7:49950104-49950123 | None:intergenic | 30.0% | |
| ! | GAAGGATAAAAAAAAGTGTG+AGG | + | Chr7:49950012-49950031 | None:intergenic | 30.0% |
| !! | ATAAATTGATGATGAGGAGA+AGG | + | Chr7:49950030-49950049 | None:intergenic | 30.0% |
| !!! | AAATTGAGTGGTTTTGAGAT+AGG | + | Chr7:49949980-49949999 | None:intergenic | 30.0% |
| AACTGAATCGAAATCAACCT+CGG | - | Chr7:49950108-49950127 | MsG0780038613.01.T02:CDS | 35.0% | |
| AGGAAGTGAAAGAAATTGAG+TGG | + | Chr7:49949992-49950011 | None:intergenic | 35.0% | |
| GTCCAAACCCTATTTCAAAT+CGG | - | Chr7:49950221-49950240 | MsG0780038613.01.T02:CDS | 35.0% | |
| TCATAGAAGAAGGGTTTGTA+GGG | + | Chr7:49950075-49950094 | None:intergenic | 35.0% | |
| TGGTAGAAGTCATAGAAGAA+GGG | + | Chr7:49950084-49950103 | None:intergenic | 35.0% | |
| TTGGTAGAAGTCATAGAAGA+AGG | + | Chr7:49950085-49950104 | None:intergenic | 35.0% | |
| ! | CATCCTTTTGTGTTTTGTGT+GGG | + | Chr7:49950156-49950175 | None:intergenic | 35.0% |
| !! | GCTGAGATAAATTGATGATG+AGG | + | Chr7:49950036-49950055 | None:intergenic | 35.0% |
| !! | TACCGATTTGAAATAGGGTT+TGG | + | Chr7:49950226-49950245 | None:intergenic | 35.0% |
| AAAACACAAAAGGATGGAGC+TGG | - | Chr7:49950160-49950179 | MsG0780038613.01.T02:CDS | 40.0% | |
| CCACACAAAACACAAAAGGA+TGG | - | Chr7:49950154-49950173 | MsG0780038613.01.T02:CDS | 40.0% | |
| GATGGAGCTGGAAAAGAATT+TGG | - | Chr7:49950172-49950191 | MsG0780038613.01.T02:CDS | 40.0% | |
| GTCATAGAAGAAGGGTTTGT+AGG | + | Chr7:49950076-49950095 | None:intergenic | 40.0% | |
| TTTGTAGGGTTTGTGGTTGT+TGG | + | Chr7:49950061-49950080 | None:intergenic | 40.0% | |
| ! | CCATCCTTTTGTGTTTTGTG+TGG | + | Chr7:49950157-49950176 | None:intergenic | 40.0% |
| ! | GAGATAGGTTGTGAGGTATT+AGG | + | Chr7:49949965-49949984 | None:intergenic | 40.0% |
| !!! | TGGTTTTGAGATAGGTTGTG+AGG | + | Chr7:49949972-49949991 | None:intergenic | 40.0% |
| AGAAGGGTTTGTAGGGTTTG+TGG | + | Chr7:49950068-49950087 | None:intergenic | 45.0% | |
| GAAATAGGGTTTGGACCACT+TGG | + | Chr7:49950217-49950236 | None:intergenic | 45.0% | |
| GATGCTCATGAAGTTCCAAG+TGG | - | Chr7:49950199-49950218 | MsG0780038613.01.T02:CDS | 45.0% | |
| GCACCCACACAAAACACAAA+AGG | - | Chr7:49950150-49950169 | MsG0780038613.01.T02:CDS | 45.0% | |
| ! | ATTGAACCCTTGTGGGCTAT+TGG | - | Chr7:49949905-49949924 | MsG0780038613.01.T02:CDS | 45.0% |
| ! | TTGAACCCTTGTGGGCTATT+GGG | - | Chr7:49949906-49949925 | MsG0780038613.01.T02:CDS | 45.0% |
| ! | TGAACCCTTGTGGGCTATTG+GGG | - | Chr7:49949907-49949926 | MsG0780038613.01.T02:CDS | 50.0% |
| GTTTGTCGTAGATGTCGCCG+AGG | + | Chr7:49950128-49950147 | None:intergenic | 55.0% | |
| ! | AACCCTTGTGGGCTATTGGG+GGG | - | Chr7:49949909-49949928 | MsG0780038613.01.T02:CDS | 55.0% |
| ! | GAACCCTTGTGGGCTATTGG+GGG | - | Chr7:49949908-49949927 | MsG0780038613.01.T02:CDS | 55.0% |
| CACCCCCCAATAGCCCACAA+GGG | + | Chr7:49949914-49949933 | None:intergenic | 60.0% | |
| CCACCCCCCAATAGCCCACA+AGG | + | Chr7:49949915-49949934 | None:intergenic | 65.0% | |
| ! | CCTTGTGGGCTATTGGGGGG+TGG | - | Chr7:49949912-49949931 | MsG0780038613.01.T02:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 49949899 | 49950246 | 49949899 | ID=MsG0780038613.01;Name=MsG0780038613.01 |
| Chr7 | mRNA | 49949899 | 49950246 | 49949899 | ID=MsG0780038613.01.T01;Parent=MsG0780038613.01;Name=MsG0780038613.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|105 |
| Chr7 | exon | 49949899 | 49949985 | 49949899 | ID=MsG0780038613.01.T01:exon:14703;Parent=MsG0780038613.01.T01 |
| Chr7 | exon | 49950016 | 49950246 | 49950016 | ID=MsG0780038613.01.T01:exon:14702;Parent=MsG0780038613.01.T01 |
| Chr7 | CDS | 49950016 | 49950246 | 49950016 | ID=MsG0780038613.01.T01:cds;Parent=MsG0780038613.01.T01 |
| Chr7 | CDS | 49949899 | 49949985 | 49949899 | ID=MsG0780038613.01.T01:cds;Parent=MsG0780038613.01.T01 |
| Chr7 | mRNA | 49949899 | 49950246 | 49949899 | ID=MsG0780038613.01.T02;Parent=MsG0780038613.01;Name=MsG0780038613.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|115 |
| Chr7 | exon | 49949899 | 49950246 | 49949899 | ID=MsG0780038613.01.T02:exon:14704;Parent=MsG0780038613.01.T02 |
| Chr7 | CDS | 49949899 | 49950246 | 49949899 | ID=MsG0780038613.01.T02:cds;Parent=MsG0780038613.01.T02 |
| Gene Sequence |
| Protein sequence |
