Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038726.01.T01 | XP_003623058.1 | 95.146 | 206 | 7 | 1 | 1 | 203 | 1 | 206 | 4.36E-144 | 409 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038726.01.T01 | Q9SIB4 | 83.784 | 74 | 12 | 0 | 1 | 74 | 1 | 74 | 1.25E-39 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038726.01.T01 | G7L3H0 | 95.146 | 206 | 7 | 1 | 1 | 203 | 1 | 206 | 2.08e-144 | 409 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780038726.01.T01 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680035683.01 | MsG0780038726.01 | 0.802649 | 5.075423e-49 | 2.499523e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038726.01.T01 | MTR_7g060630 | 95.146 | 206 | 7 | 1 | 1 | 203 | 1 | 206 | 5.28e-148 | 409 |
| MsG0780038726.01.T01 | MTR_4g084550 | 71.429 | 70 | 20 | 0 | 1 | 70 | 57 | 126 | 1.83e-30 | 110 |
| MsG0780038726.01.T01 | MTR_1g019130 | 71.642 | 67 | 19 | 0 | 4 | 70 | 86 | 152 | 1.67e-28 | 107 |
| MsG0780038726.01.T01 | MTR_1g019130 | 71.642 | 67 | 19 | 0 | 4 | 70 | 86 | 152 | 1.82e-28 | 107 |
| MsG0780038726.01.T01 | MTR_8g107210 | 74.627 | 67 | 17 | 0 | 6 | 72 | 94 | 160 | 4.77e-28 | 108 |
| MsG0780038726.01.T01 | MTR_5g081990 | 69.841 | 63 | 19 | 0 | 8 | 70 | 33 | 95 | 1.61e-27 | 103 |
| MsG0780038726.01.T01 | MTR_4g063735 | 59.740 | 77 | 27 | 1 | 8 | 80 | 25 | 101 | 1.03e-24 | 97.8 |
| MsG0780038726.01.T01 | MTR_5g021930 | 67.692 | 65 | 20 | 1 | 6 | 69 | 28 | 92 | 7.12e-24 | 96.3 |
| MsG0780038726.01.T01 | MTR_8g095580 | 74.074 | 54 | 14 | 0 | 19 | 72 | 93 | 146 | 3.66e-21 | 86.3 |
| MsG0780038726.01.T01 | MTR_3g088485 | 73.585 | 53 | 14 | 0 | 19 | 71 | 91 | 143 | 1.55e-20 | 84.3 |
| MsG0780038726.01.T01 | MTR_7g025010 | 39.535 | 86 | 52 | 0 | 2 | 87 | 41 | 126 | 1.90e-14 | 71.6 |
| MsG0780038726.01.T01 | MTR_2g015000 | 45.588 | 68 | 37 | 0 | 2 | 69 | 49 | 116 | 3.16e-14 | 70.9 |
| MsG0780038726.01.T01 | MTR_1g115315 | 46.774 | 62 | 33 | 0 | 8 | 69 | 85 | 146 | 1.10e-12 | 65.5 |
| MsG0780038726.01.T01 | MTR_1g115315 | 46.774 | 62 | 33 | 0 | 8 | 69 | 85 | 146 | 1.93e-12 | 64.7 |
| MsG0780038726.01.T01 | MTR_4g088070 | 43.077 | 65 | 36 | 1 | 4 | 67 | 24 | 88 | 7.48e-12 | 63.9 |
| MsG0780038726.01.T01 | MTR_4g088080 | 35.802 | 81 | 51 | 1 | 1 | 81 | 33 | 112 | 6.72e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038726.01.T01 | AT2G28610 | 83.784 | 74 | 12 | 0 | 1 | 74 | 1 | 74 | 1.28e-40 | 138 |
| MsG0780038726.01.T01 | AT3G18010 | 73.134 | 67 | 18 | 0 | 6 | 72 | 74 | 140 | 7.01e-28 | 107 |
| MsG0780038726.01.T01 | AT3G11260 | 69.841 | 63 | 19 | 0 | 8 | 70 | 24 | 86 | 2.67e-27 | 102 |
| MsG0780038726.01.T01 | AT1G46480 | 67.164 | 67 | 22 | 0 | 4 | 70 | 86 | 152 | 3.96e-27 | 102 |
| MsG0780038726.01.T01 | AT1G46480 | 67.164 | 67 | 22 | 0 | 4 | 70 | 86 | 152 | 2.05e-26 | 102 |
| MsG0780038726.01.T01 | AT5G59340 | 65.333 | 75 | 22 | 1 | 6 | 76 | 12 | 86 | 3.07e-26 | 101 |
| MsG0780038726.01.T01 | AT5G05770 | 65.079 | 63 | 22 | 0 | 8 | 70 | 29 | 91 | 3.38e-26 | 97.8 |
| MsG0780038726.01.T01 | AT2G17950 | 67.692 | 65 | 20 | 1 | 6 | 69 | 36 | 100 | 1.80e-23 | 95.1 |
| MsG0780038726.01.T01 | AT2G01500 | 74.545 | 55 | 14 | 0 | 8 | 62 | 61 | 115 | 1.30e-22 | 92.4 |
| MsG0780038726.01.T01 | AT5G45980 | 47.761 | 67 | 35 | 0 | 3 | 69 | 50 | 116 | 3.64e-14 | 70.1 |
| MsG0780038726.01.T01 | AT2G33880 | 44.444 | 72 | 40 | 0 | 2 | 73 | 49 | 120 | 4.25e-14 | 70.1 |
| MsG0780038726.01.T01 | AT2G33880 | 44.444 | 72 | 40 | 0 | 2 | 73 | 49 | 120 | 4.51e-14 | 70.1 |
| MsG0780038726.01.T01 | AT1G20700 | 48.333 | 60 | 31 | 0 | 8 | 67 | 33 | 92 | 3.35e-13 | 64.7 |
| MsG0780038726.01.T01 | AT1G20700 | 48.333 | 60 | 31 | 0 | 8 | 67 | 95 | 154 | 9.08e-13 | 64.7 |
| MsG0780038726.01.T01 | AT1G20700 | 48.333 | 60 | 31 | 0 | 8 | 67 | 95 | 154 | 1.10e-12 | 64.7 |
| MsG0780038726.01.T01 | AT4G35550 | 48.333 | 60 | 31 | 0 | 8 | 67 | 99 | 158 | 3.57e-12 | 63.9 |
Find 33 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACTCACCTTTCTTTCTA+TGG | 0.291926 | 7:-51963799 | MsG0780038726.01.T01:CDS |
| TCTTTCTATGGTAGGATAGA+AGG | 0.293221 | 7:-51963787 | MsG0780038726.01.T01:CDS |
| GAGATTGCATTCAACAACTA+TGG | 0.335765 | 7:-51962929 | MsG0780038726.01.T01:CDS |
| CAAAAGTACTACCAACAAAA+TGG | 0.345039 | 7:+51963608 | None:intergenic |
| TCTTCTCCATCAAACTTTGT+TGG | 0.346374 | 7:+51962954 | None:intergenic |
| TGAACAACTTATGATCTTAG+AGG | 0.352994 | 7:-51963875 | MsG0780038726.01.T01:CDS |
| TAATAACCAACAAAGTTTGA+TGG | 0.363815 | 7:-51962960 | MsG0780038726.01.T01:CDS |
| ATCATAAGTTGTTCAGTTGT+AGG | 0.386722 | 7:+51963883 | None:intergenic |
| AAGGGGCAATAAATTGCAAA+TGG | 0.472403 | 7:-51962991 | MsG0780038726.01.T01:CDS |
| TCTTGTGCATTAGATCCAAA+AGG | 0.476916 | 7:+51963580 | None:intergenic |
| CTATCCTACCATAGAAAGAA+AGG | 0.481976 | 7:+51963791 | None:intergenic |
| TCATAAGTTGTTCAGTTGTA+GGG | 0.482629 | 7:+51963884 | None:intergenic |
| CTCCACCTGGTTCATCAAGA+TGG | 0.487034 | 7:-51963909 | MsG0780038726.01.T01:CDS |
| GGGATAGACAGAAGTTAAGA+AGG | 0.493019 | 7:-51963726 | MsG0780038726.01.T01:CDS |
| CTATTGGTTTCAGAATCATA+AGG | 0.495866 | 7:-51963752 | MsG0780038726.01.T01:CDS |
| GGTTTCAGAATCATAAGGCT+AGG | 0.507758 | 7:-51963747 | MsG0780038726.01.T01:CDS |
| TTGCATTCAACAACTATGGT+TGG | 0.509929 | 7:-51962925 | MsG0780038726.01.T01:CDS |
| CTATGGTTGGACAACACTCA+TGG | 0.513146 | 7:-51962912 | MsG0780038726.01.T01:CDS |
| CTCACCTTTCTTTCTATGGT+AGG | 0.541469 | 7:-51963795 | MsG0780038726.01.T01:CDS |
| TGCTGAATTTGAACAGCACT+AGG | 0.547085 | 7:+51963826 | None:intergenic |
| TTGATTCTTGTTGTTGTGAG+AGG | 0.549477 | 7:+51962827 | None:intergenic |
| CTTGTGCATTAGATCCAAAA+GGG | 0.549702 | 7:+51963581 | None:intergenic |
| GTTTCAGAATCATAAGGCTA+GGG | 0.551754 | 7:-51963746 | MsG0780038726.01.T01:CDS |
| GCAATAAATTGCAAATGGAA+TGG | 0.553921 | 7:-51962986 | MsG0780038726.01.T01:CDS |
| GTAGCTGCAAACACATCAGA+AGG | 0.562740 | 7:-51963010 | MsG0780038726.01.T01:CDS |
| AGAGGTTTAGTGCAACATGA+AGG | 0.572055 | 7:+51962869 | None:intergenic |
| TTAGAGGAACTGTATAGAAG+TGG | 0.579089 | 7:-51963859 | MsG0780038726.01.T01:CDS |
| CAATCAATAATGTCTCCACC+TGG | 0.600364 | 7:-51963922 | None:intergenic |
| AAAAGATCAAGAGTCTTGAG+AGG | 0.601753 | 7:+51962851 | None:intergenic |
| GGGCTCCATCTTGATGAACC+AGG | 0.608838 | 7:+51963904 | None:intergenic |
| TAGCTGCAAACACATCAGAA+GGG | 0.621365 | 7:-51963009 | MsG0780038726.01.T01:CDS |
| AGCTGCAAACACATCAGAAG+GGG | 0.625629 | 7:-51963008 | MsG0780038726.01.T01:CDS |
| CTCCATCTTGATGAACCAGG+TGG | 0.692416 | 7:+51963907 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAATATATATATATAT+AGG | - | Chr7:51963602-51963621 | MsG0780038726.01.T01:CDS | 0.0% |
| !! | ATTAAATATATATATATATA+GGG | - | Chr7:51963603-51963622 | MsG0780038726.01.T01:CDS | 0.0% |
| !! | AAAGATGATAATTTAAGTCA+TGG | + | Chr7:51963494-51963513 | None:intergenic | 20.0% |
| !! | AAATTTCTTGACTTAAATCA+TGG | - | Chr7:51963332-51963351 | MsG0780038726.01.T01:intron | 20.0% |
| !! | AGAAAAAGAAGAAGAAAAAT+AGG | + | Chr7:51963273-51963292 | None:intergenic | 20.0% |
| !! | ATGACTTAAATTATCATCTT+TGG | - | Chr7:51963493-51963512 | MsG0780038726.01.T01:intron | 20.0% |
| !! | CTTCATTTGATATGTATTTA+GGG | - | Chr7:51963696-51963715 | MsG0780038726.01.T01:CDS | 20.0% |
| !! | TAATTCTTCTTTATAACTCT+TGG | - | Chr7:51963302-51963321 | MsG0780038726.01.T01:intron | 20.0% |
| !! | TCTTCATTTGATATGTATTT+AGG | - | Chr7:51963695-51963714 | MsG0780038726.01.T01:CDS | 20.0% |
| !!! | AAGGAAAAAATGTTTTCTAT+TGG | - | Chr7:51962965-51962984 | MsG0780038726.01.T01:CDS | 20.0% |
| !!! | TAAATCATGGTTTTAAATTG+CGG | - | Chr7:51963345-51963364 | MsG0780038726.01.T01:intron | 20.0% |
| ! | GAAGAAAAATAGGACAAAAA+AGG | + | Chr7:51963263-51963282 | None:intergenic | 25.0% |
| ! | TAAATTATCATCTTTGGTTG+TGG | - | Chr7:51963499-51963518 | MsG0780038726.01.T01:intron | 25.0% |
| ! | TAATAACCAACAAAGTTTGA+TGG | - | Chr7:51963773-51963792 | MsG0780038726.01.T01:CDS | 25.0% |
| !! | AGCTACGAATTTTTATATAG+TGG | + | Chr7:51963443-51963462 | None:intergenic | 25.0% |
| ATCATAAGTTGTTCAGTTGT+AGG | + | Chr7:51962853-51962872 | None:intergenic | 30.0% | |
| GCAATAAATTGCAAATGGAA+TGG | - | Chr7:51963747-51963766 | MsG0780038726.01.T01:CDS | 30.0% | |
| TCATAAGTTGTTCAGTTGTA+GGG | + | Chr7:51962852-51962871 | None:intergenic | 30.0% | |
| TGAACAACTTATGATCTTAG+AGG | - | Chr7:51962858-51962877 | MsG0780038726.01.T01:CDS | 30.0% | |
| ! | ATGACTAGTAACCATTTTGT+TGG | - | Chr7:51963114-51963133 | MsG0780038726.01.T01:intron | 30.0% |
| ! | CTGCGATATAAAAGATTTTG+AGG | - | Chr7:51963387-51963406 | MsG0780038726.01.T01:intron | 30.0% |
| !! | CAAAAGTACTACCAACAAAA+TGG | + | Chr7:51963128-51963147 | None:intergenic | 30.0% |
| !! | CTATTGGTTTCAGAATCATA+AGG | - | Chr7:51962981-51963000 | MsG0780038726.01.T01:CDS | 30.0% |
| !!! | CATTTTGTTGGTAGTACTTT+TGG | - | Chr7:51963126-51963145 | MsG0780038726.01.T01:intron | 30.0% |
| AAAAGATCAAGAGTCTTGAG+AGG | + | Chr7:51963885-51963904 | None:intergenic | 35.0% | |
| AAGGGGCAATAAATTGCAAA+TGG | - | Chr7:51963742-51963761 | MsG0780038726.01.T01:CDS | 35.0% | |
| AATCCAATCCTTCTGTGTAA+TGG | + | Chr7:51963577-51963596 | None:intergenic | 35.0% | |
| ACTACTCACCTTTCTTTCTA+TGG | - | Chr7:51962934-51962953 | MsG0780038726.01.T01:CDS | 35.0% | |
| ATTACCTCAAAAAGCAAGCT+AGG | + | Chr7:51963198-51963217 | None:intergenic | 35.0% | |
| CAAGCTAGGTGAGTTAAAAA+AGG | + | Chr7:51963184-51963203 | None:intergenic | 35.0% | |
| CTATCCTACCATAGAAAGAA+AGG | + | Chr7:51962945-51962964 | None:intergenic | 35.0% | |
| CTTGTGCATTAGATCCAAAA+GGG | + | Chr7:51963155-51963174 | None:intergenic | 35.0% | |
| GAAAGTTGCGATTGAAATTG+TGG | + | Chr7:51963421-51963440 | None:intergenic | 35.0% | |
| GAGATTGCATTCAACAACTA+TGG | - | Chr7:51963804-51963823 | MsG0780038726.01.T01:CDS | 35.0% | |
| GTTTCAGAATCATAAGGCTA+GGG | - | Chr7:51962987-51963006 | MsG0780038726.01.T01:CDS | 35.0% | |
| TCTTCTCCATCAAACTTTGT+TGG | + | Chr7:51963782-51963801 | None:intergenic | 35.0% | |
| TCTTGTGCATTAGATCCAAA+AGG | + | Chr7:51963156-51963175 | None:intergenic | 35.0% | |
| TCTTTCTATGGTAGGATAGA+AGG | - | Chr7:51962946-51962965 | MsG0780038726.01.T01:CDS | 35.0% | |
| TGAATTCAAACAATGCCACT+TGG | - | Chr7:51963551-51963570 | MsG0780038726.01.T01:CDS | 35.0% | |
| TTAGAGGAACTGTATAGAAG+TGG | - | Chr7:51962874-51962893 | MsG0780038726.01.T01:CDS | 35.0% | |
| TTGCATTCAACAACTATGGT+TGG | - | Chr7:51963808-51963827 | MsG0780038726.01.T01:CDS | 35.0% | |
| !! | AGTACTTTTGGTTACCCTTT+TGG | - | Chr7:51963138-51963157 | MsG0780038726.01.T01:intron | 35.0% |
| !! | TTGATTCTTGTTGTTGTGAG+AGG | + | Chr7:51963909-51963928 | None:intergenic | 35.0% |
| AGAGGTTTAGTGCAACATGA+AGG | + | Chr7:51963867-51963886 | None:intergenic | 40.0% | |
| CTCACCTTTCTTTCTATGGT+AGG | - | Chr7:51962938-51962957 | MsG0780038726.01.T01:CDS | 40.0% | |
| GCGGTCTGTAACTTCAATTA+CGG | - | Chr7:51963364-51963383 | MsG0780038726.01.T01:intron | 40.0% | |
| GGGATAGACAGAAGTTAAGA+AGG | - | Chr7:51963007-51963026 | MsG0780038726.01.T01:CDS | 40.0% | |
| GGTTTCAGAATCATAAGGCT+AGG | - | Chr7:51962986-51963005 | MsG0780038726.01.T01:CDS | 40.0% | |
| TAGCTGCAAACACATCAGAA+GGG | - | Chr7:51963724-51963743 | MsG0780038726.01.T01:CDS | 40.0% | |
| TGCTGAATTTGAACAGCACT+AGG | + | Chr7:51962910-51962929 | None:intergenic | 40.0% | |
| TTAAATTGCAGTCGCAGTTG+CGG | + | Chr7:51963469-51963488 | None:intergenic | 40.0% | |
| AGCTGCAAACACATCAGAAG+GGG | - | Chr7:51963725-51963744 | MsG0780038726.01.T01:CDS | 45.0% | |
| CTATGGTTGGACAACACTCA+TGG | - | Chr7:51963821-51963840 | MsG0780038726.01.T01:CDS | 45.0% | |
| GTAGCTGCAAACACATCAGA+AGG | - | Chr7:51963723-51963742 | MsG0780038726.01.T01:CDS | 45.0% | |
| TGGCCATTACACAGAAGGAT+TGG | - | Chr7:51963571-51963590 | MsG0780038726.01.T01:CDS | 45.0% | |
| !!! | CTCACCTAGCTTGCTTTTTG+AGG | - | Chr7:51963191-51963210 | MsG0780038726.01.T01:intron | 45.0% |
| CCACTTGGCCATTACACAGA+AGG | - | Chr7:51963566-51963585 | MsG0780038726.01.T01:CDS | 50.0% | |
| CCTTCTGTGTAATGGCCAAG+TGG | + | Chr7:51963569-51963588 | None:intergenic | 50.0% | |
| CTCCACCTGGTTCATCAAGA+TGG | - | Chr7:51962824-51962843 | MsG0780038726.01.T01:CDS | 50.0% | |
| CTCCATCTTGATGAACCAGG+TGG | + | Chr7:51962829-51962848 | None:intergenic | 50.0% | |
| GGGCTCCATCTTGATGAACC+AGG | + | Chr7:51962832-51962851 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 51962820 | 51963935 | 51962820 | ID=MsG0780038726.01;Name=MsG0780038726.01 |
| Chr7 | mRNA | 51962820 | 51963935 | 51962820 | ID=MsG0780038726.01.T01;Parent=MsG0780038726.01;Name=MsG0780038726.01.T01;_AED=0.33;_eAED=0.34;_QI=0|0|0|1|1|1|2|0|203 |
| Chr7 | exon | 51963543 | 51963935 | 51963543 | ID=MsG0780038726.01.T01:exon:8199;Parent=MsG0780038726.01.T01 |
| Chr7 | exon | 51962820 | 51963038 | 51962820 | ID=MsG0780038726.01.T01:exon:8198;Parent=MsG0780038726.01.T01 |
| Chr7 | CDS | 51963543 | 51963935 | 51963543 | ID=MsG0780038726.01.T01:cds;Parent=MsG0780038726.01.T01 |
| Chr7 | CDS | 51962820 | 51963038 | 51962820 | ID=MsG0780038726.01.T01:cds;Parent=MsG0780038726.01.T01 |
| Gene Sequence |
| Protein sequence |
