Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039002.01.T01 | KEH23089.1 | 89.091 | 110 | 12 | 0 | 1 | 110 | 297 | 406 | 3.25E-59 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039002.01.T01 | A0A072TZY4 | 89.091 | 110 | 12 | 0 | 1 | 110 | 297 | 406 | 1.55e-59 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780039002.01 | MsG0880045023.01 | 0.833420 | 5.387019e-56 | 6.200792e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039002.01.T01 | MTR_7g066310 | 89.091 | 110 | 12 | 0 | 1 | 110 | 297 | 406 | 3.93e-63 | 197 |
| MsG0780039002.01.T01 | MTR_7g066280 | 86.364 | 110 | 15 | 0 | 1 | 110 | 304 | 413 | 2.82e-60 | 190 |
| MsG0780039002.01.T01 | MTR_7g066340 | 87.156 | 109 | 14 | 0 | 2 | 110 | 368 | 476 | 7.56e-60 | 190 |
| MsG0780039002.01.T01 | MTR_7g066390 | 72.043 | 93 | 26 | 0 | 3 | 95 | 38 | 130 | 1.42e-43 | 139 |
| MsG0780039002.01.T01 | MTR_7g066260 | 70.408 | 98 | 29 | 0 | 4 | 101 | 379 | 476 | 1.61e-41 | 142 |
| MsG0780039002.01.T01 | MTR_7g066360 | 60.606 | 99 | 39 | 0 | 3 | 101 | 365 | 463 | 4.22e-36 | 128 |
| MsG0780039002.01.T01 | MTR_7g066350 | 69.892 | 93 | 23 | 1 | 4 | 91 | 366 | 458 | 6.62e-34 | 122 |
| MsG0780039002.01.T01 | MTR_7g066400 | 72.840 | 81 | 22 | 0 | 3 | 83 | 353 | 433 | 8.29e-34 | 121 |
| MsG0780039002.01.T01 | MTR_1g041315 | 69.118 | 68 | 21 | 0 | 4 | 71 | 384 | 451 | 6.79e-28 | 105 |
| MsG0780039002.01.T01 | MTR_7g066290 | 76.923 | 65 | 15 | 0 | 24 | 88 | 370 | 434 | 4.05e-27 | 103 |
| MsG0780039002.01.T01 | MTR_7g066570 | 56.000 | 100 | 41 | 2 | 4 | 102 | 396 | 493 | 5.08e-26 | 100 |
| MsG0780039002.01.T01 | MTR_7g066580 | 56.250 | 96 | 33 | 2 | 3 | 93 | 356 | 447 | 6.01e-24 | 94.7 |
| MsG0780039002.01.T01 | MTR_7g058710 | 53.333 | 90 | 37 | 1 | 3 | 87 | 388 | 477 | 2.49e-22 | 90.5 |
| MsG0780039002.01.T01 | MTR_1g014060 | 56.579 | 76 | 33 | 0 | 18 | 93 | 315 | 390 | 1.19e-21 | 88.2 |
| MsG0780039002.01.T01 | MTR_7g066410 | 45.263 | 95 | 44 | 1 | 1 | 87 | 418 | 512 | 6.99e-20 | 83.6 |
| MsG0780039002.01.T01 | MTR_7g066680 | 44.068 | 118 | 30 | 2 | 3 | 84 | 218 | 335 | 1.64e-19 | 81.6 |
| MsG0780039002.01.T01 | MTR_7g058700 | 54.054 | 74 | 34 | 0 | 14 | 87 | 848 | 921 | 2.80e-19 | 82.0 |
| MsG0780039002.01.T01 | MTR_7g066430 | 46.237 | 93 | 42 | 1 | 3 | 87 | 393 | 485 | 4.12e-19 | 81.3 |
| MsG0780039002.01.T01 | MTR_7g066480 | 51.389 | 72 | 35 | 0 | 18 | 89 | 352 | 423 | 8.49e-19 | 80.5 |
| MsG0780039002.01.T01 | MTR_7g066490 | 51.389 | 72 | 35 | 0 | 18 | 89 | 338 | 409 | 1.69e-18 | 79.3 |
| MsG0780039002.01.T01 | MTR_7g012680 | 49.425 | 87 | 43 | 1 | 7 | 93 | 366 | 451 | 6.55e-18 | 77.8 |
| MsG0780039002.01.T01 | MTR_7g062280 | 50.685 | 73 | 36 | 0 | 15 | 87 | 413 | 485 | 8.75e-18 | 77.4 |
| MsG0780039002.01.T01 | MTR_4g019310 | 47.727 | 88 | 40 | 2 | 2 | 83 | 399 | 486 | 8.80e-18 | 77.4 |
| MsG0780039002.01.T01 | MTR_2g029400 | 53.333 | 75 | 30 | 1 | 6 | 75 | 329 | 403 | 7.98e-17 | 74.7 |
| MsG0780039002.01.T01 | MTR_4g017830 | 41.667 | 84 | 49 | 0 | 15 | 98 | 429 | 512 | 1.13e-15 | 71.6 |
| MsG0780039002.01.T01 | MTR_7g062300 | 52.174 | 69 | 32 | 1 | 16 | 83 | 401 | 469 | 3.05e-15 | 70.5 |
| MsG0780039002.01.T01 | MTR_7g100420 | 44.706 | 85 | 44 | 1 | 6 | 87 | 313 | 397 | 5.18e-15 | 69.7 |
| MsG0780039002.01.T01 | MTR_7g063920 | 43.836 | 73 | 41 | 0 | 15 | 87 | 358 | 430 | 9.50e-15 | 68.9 |
| MsG0780039002.01.T01 | MTR_7g063820 | 46.250 | 80 | 42 | 1 | 9 | 87 | 399 | 478 | 1.59e-14 | 68.2 |
| MsG0780039002.01.T01 | MTR_3g065200 | 47.297 | 74 | 37 | 1 | 3 | 74 | 335 | 408 | 3.78e-14 | 67.4 |
| MsG0780039002.01.T01 | MTR_7g063880 | 42.169 | 83 | 46 | 1 | 8 | 88 | 381 | 463 | 5.60e-14 | 66.6 |
| MsG0780039002.01.T01 | MTR_7g063720 | 45.205 | 73 | 40 | 0 | 15 | 87 | 154 | 226 | 1.06e-13 | 65.1 |
| MsG0780039002.01.T01 | MTR_7g063670 | 42.667 | 75 | 42 | 1 | 14 | 87 | 377 | 451 | 1.97e-13 | 65.1 |
| MsG0780039002.01.T01 | MTR_8g445860 | 44.444 | 72 | 39 | 1 | 10 | 80 | 160 | 231 | 2.68e-13 | 63.9 |
| MsG0780039002.01.T01 | MTR_7g063850 | 45.946 | 74 | 39 | 1 | 15 | 87 | 209 | 282 | 3.26e-13 | 63.9 |
| MsG0780039002.01.T01 | MTR_7g063760 | 43.939 | 66 | 36 | 1 | 23 | 87 | 2 | 67 | 1.84e-12 | 58.2 |
| MsG0780039002.01.T01 | MTR_7g063660 | 38.356 | 73 | 45 | 0 | 15 | 87 | 399 | 471 | 2.17e-12 | 62.4 |
| MsG0780039002.01.T01 | MTR_0150s0010 | 41.026 | 78 | 45 | 1 | 4 | 80 | 154 | 231 | 3.83e-12 | 60.8 |
| MsG0780039002.01.T01 | MTR_3g007740 | 42.466 | 73 | 41 | 1 | 16 | 87 | 370 | 442 | 1.88e-11 | 59.7 |
| MsG0780039002.01.T01 | MTR_4g017610 | 38.554 | 83 | 50 | 1 | 2 | 84 | 395 | 476 | 2.20e-11 | 59.3 |
| MsG0780039002.01.T01 | MTR_8g044880 | 39.506 | 81 | 46 | 2 | 4 | 81 | 192 | 272 | 3.49e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAGCACCATTAACGTTTA+TGG | 0.174129 | 7:-56653785 | MsG0780039002.01.T01:CDS |
| GATCCTAAATCCCCTATTTC+TGG | 0.194829 | 7:-56653817 | MsG0780039002.01.T01:CDS |
| CTAGCATATCCTCCAGAAAT+AGG | 0.222209 | 7:+56653805 | None:intergenic |
| CCTCCAGAAATAGGGGATTT+AGG | 0.261981 | 7:+56653814 | None:intergenic |
| TCAAAATTTGCTTTGACAAA+CGG | 0.362323 | 7:-56653525 | MsG0780039002.01.T01:CDS |
| TAGCATATCCTCCAGAAATA+GGG | 0.363628 | 7:+56653806 | None:intergenic |
| GAGGAAATGGTCGTTAATAT+TGG | 0.365896 | 7:-56653676 | MsG0780039002.01.T01:CDS |
| AACGGCCTCAACCAGTTCAT+TGG | 0.391379 | 7:-56653507 | MsG0780039002.01.T01:intron |
| GGCAGGAAGTAGATCTGTTC+AGG | 0.408889 | 7:-56653052 | MsG0780039002.01.T01:intron |
| TCTGTCACCATAAACGTTAA+TGG | 0.411866 | 7:+56653778 | None:intergenic |
| AAGATATGAAATACCATCAA+TGG | 0.434003 | 7:+56653729 | None:intergenic |
| TACTATACTAACCAATGAAC+TGG | 0.434085 | 7:+56653496 | None:intergenic |
| ACTTAATGATGTGGAGGAAA+TGG | 0.448325 | 7:-56653689 | MsG0780039002.01.T01:CDS |
| ACTCCAATGCCATCCATTGA+TGG | 0.490084 | 7:-56653742 | MsG0780039002.01.T01:CDS |
| ATTTCCTCCACATCATTAAG+TGG | 0.492887 | 7:+56653691 | None:intergenic |
| GCATTGGAGTCACAACCAAC+TGG | 0.508849 | 7:+56653755 | None:intergenic |
| ATACCATCAATGGATGGCAT+TGG | 0.542259 | 7:+56653739 | None:intergenic |
| ACTAACCAATGAACTGGTTG+AGG | 0.567961 | 7:+56653502 | None:intergenic |
| AGCATATCCTCCAGAAATAG+GGG | 0.573043 | 7:+56653807 | None:intergenic |
| TATGAAATACCATCAATGGA+TGG | 0.601386 | 7:+56653733 | None:intergenic |
| GTTTATGGTGACAGACCAGT+TGG | 0.602694 | 7:-56653770 | MsG0780039002.01.T01:CDS |
| CGTTAATATTGGCCAGAAGG+AGG | 0.604404 | 7:-56653665 | MsG0780039002.01.T01:intron |
| GGTCGTTAATATTGGCCAGA+AGG | 0.607810 | 7:-56653668 | MsG0780039002.01.T01:CDS |
| GAACGTCCCACTTAATGATG+TGG | 0.609331 | 7:-56653698 | MsG0780039002.01.T01:CDS |
| CGTCCCACTTAATGATGTGG+AGG | 0.619494 | 7:-56653695 | MsG0780039002.01.T01:CDS |
| TTTCCTCCACATCATTAAGT+GGG | 0.624477 | 7:+56653692 | None:intergenic |
| CCTAAATCCCCTATTTCTGG+AGG | 0.628731 | 7:-56653814 | MsG0780039002.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGTTATAATTATTATGT+AGG | + | Chr7:56653385-56653404 | None:intergenic | 10.0% |
| !! | TATAATTATTATGTAGGATG+AGG | + | Chr7:56653379-56653398 | None:intergenic | 20.0% |
| ! | AAGATATGAAATACCATCAA+TGG | + | Chr7:56653108-56653127 | None:intergenic | 25.0% |
| ! | TAGAAAAAGAAGAGAAGTAA+AGG | - | Chr7:56653661-56653680 | MsG0780039002.01.T01:intron | 25.0% |
| ! | TGAACATGCACATATAATAA+AGG | + | Chr7:56653761-56653780 | None:intergenic | 25.0% |
| ! | TTATTATATGTGCATGTTCA+TGG | - | Chr7:56653761-56653780 | MsG0780039002.01.T01:CDS | 25.0% |
| !! | TCAAAATTTGCTTTGACAAA+CGG | - | Chr7:56653309-56653328 | MsG0780039002.01.T01:intron | 25.0% |
| AATGAATGGTACCCATATAT+GGG | - | Chr7:56653562-56653581 | MsG0780039002.01.T01:intron | 30.0% | |
| ATATGAACATCCCCATATAT+GGG | + | Chr7:56653576-56653595 | None:intergenic | 30.0% | |
| CAACCACAAGAAATCAAATA+TGG | + | Chr7:56653256-56653275 | None:intergenic | 30.0% | |
| TACCATTCATTGCATTTACA+TGG | + | Chr7:56653553-56653572 | None:intergenic | 30.0% | |
| TACTATACTAACCAATGAAC+TGG | + | Chr7:56653341-56653360 | None:intergenic | 30.0% | |
| TAGTACTACACATAACAAGT+CGG | + | Chr7:56653525-56653544 | None:intergenic | 30.0% | |
| TATGAAATACCATCAATGGA+TGG | + | Chr7:56653104-56653123 | None:intergenic | 30.0% | |
| TTCCATGTAAATGCAATGAA+TGG | - | Chr7:56653548-56653567 | MsG0780039002.01.T01:CDS | 30.0% | |
| ! | GTTTGTCAAAGCAAATTTTG+AGG | + | Chr7:56653310-56653329 | None:intergenic | 30.0% |
| ! | TCACCATATTTGATTTCTTG+TGG | - | Chr7:56653250-56653269 | MsG0780039002.01.T01:intron | 30.0% |
| ! | TTCTACCTTTTGCATCTTTA+GGG | + | Chr7:56653832-56653851 | None:intergenic | 30.0% |
| ! | TTTCTACCTTTTGCATCTTT+AGG | + | Chr7:56653833-56653852 | None:intergenic | 30.0% |
| ACTTAATGATGTGGAGGAAA+TGG | - | Chr7:56653145-56653164 | MsG0780039002.01.T01:intron | 35.0% | |
| AGGAAGAAAGATTGAACGAA+AGG | - | Chr7:56653681-56653700 | MsG0780039002.01.T01:CDS | 35.0% | |
| ATGAATGGTACCCATATATG+GGG | - | Chr7:56653563-56653582 | MsG0780039002.01.T01:intron | 35.0% | |
| ATTTCCTCCACATCATTAAG+TGG | + | Chr7:56653146-56653165 | None:intergenic | 35.0% | |
| CAATGAATGGTACCCATATA+TGG | - | Chr7:56653561-56653580 | MsG0780039002.01.T01:intron | 35.0% | |
| CATATGAACATCCCCATATA+TGG | + | Chr7:56653577-56653596 | None:intergenic | 35.0% | |
| CATATGATCTTAGGGTAATG+AGG | - | Chr7:56653591-56653610 | MsG0780039002.01.T01:intron | 35.0% | |
| GAGGAAATGGTCGTTAATAT+TGG | - | Chr7:56653158-56653177 | MsG0780039002.01.T01:intron | 35.0% | |
| GGGATGTTCATATGATCTTA+GGG | - | Chr7:56653583-56653602 | MsG0780039002.01.T01:intron | 35.0% | |
| TAGCATATCCTCCAGAAATA+GGG | + | Chr7:56653031-56653050 | None:intergenic | 35.0% | |
| TCTGTCACCATAAACGTTAA+TGG | + | Chr7:56653059-56653078 | None:intergenic | 35.0% | |
| TTTCCTCCACATCATTAAGT+GGG | + | Chr7:56653145-56653164 | None:intergenic | 35.0% | |
| ! | ATTTGATTTCTTGTGGTTGC+AGG | - | Chr7:56653257-56653276 | MsG0780039002.01.T01:intron | 35.0% |
| ! | TAGAGCACCATTAACGTTTA+TGG | - | Chr7:56653049-56653068 | MsG0780039002.01.T01:CDS | 35.0% |
| ! | TCTACCTTTTGCATCTTTAG+GGG | + | Chr7:56653831-56653850 | None:intergenic | 35.0% |
| ! | TTTGATTTCTTGTGGTTGCA+GGG | - | Chr7:56653258-56653277 | MsG0780039002.01.T01:intron | 35.0% |
| !!! | ATGTAGGATGAGGTTTTGTT+TGG | + | Chr7:56653369-56653388 | None:intergenic | 35.0% |
| ACTAACCAATGAACTGGTTG+AGG | + | Chr7:56653335-56653354 | None:intergenic | 40.0% | |
| AGCATATCCTCCAGAAATAG+GGG | + | Chr7:56653030-56653049 | None:intergenic | 40.0% | |
| ATACCATCAATGGATGGCAT+TGG | + | Chr7:56653098-56653117 | None:intergenic | 40.0% | |
| CTAGCATATCCTCCAGAAAT+AGG | + | Chr7:56653032-56653051 | None:intergenic | 40.0% | |
| GATCCTAAATCCCCTATTTC+TGG | - | Chr7:56653017-56653036 | MsG0780039002.01.T01:CDS | 40.0% | |
| GGGGATGTTCATATGATCTT+AGG | - | Chr7:56653582-56653601 | MsG0780039002.01.T01:intron | 40.0% | |
| GTAACCACCTCTTATGTGTT+CGG | - | Chr7:56653493-56653512 | MsG0780039002.01.T01:intron | 40.0% | |
| GTTACTACCGAACACATAAG+AGG | + | Chr7:56653503-56653522 | None:intergenic | 40.0% | |
| TATATGTGCATGTTCATGGC+AGG | - | Chr7:56653765-56653784 | MsG0780039002.01.T01:CDS | 40.0% | |
| ! | CTACCTTTTGCATCTTTAGG+GGG | + | Chr7:56653830-56653849 | None:intergenic | 40.0% |
| ACTACCGAACACATAAGAGG+TGG | + | Chr7:56653500-56653519 | None:intergenic | 45.0% | |
| ACTCCAATGCCATCCATTGA+TGG | - | Chr7:56653092-56653111 | MsG0780039002.01.T01:intron | 45.0% | |
| ATTGGCCAGAAGGAGGTAAA+AGG | - | Chr7:56653176-56653195 | MsG0780039002.01.T01:intron | 45.0% | |
| CCTCCAGAAATAGGGGATTT+AGG | + | Chr7:56653023-56653042 | None:intergenic | 45.0% | |
| CGTTAATATTGGCCAGAAGG+AGG | - | Chr7:56653169-56653188 | MsG0780039002.01.T01:intron | 45.0% | |
| GAACGTCCCACTTAATGATG+TGG | - | Chr7:56653136-56653155 | MsG0780039002.01.T01:intron | 45.0% | |
| GGTCGTTAATATTGGCCAGA+AGG | - | Chr7:56653166-56653185 | MsG0780039002.01.T01:intron | 45.0% | |
| GTTTATGGTGACAGACCAGT+TGG | - | Chr7:56653064-56653083 | MsG0780039002.01.T01:intron | 45.0% | |
| TAGCCCCCTAAAGATGCAAA+AGG | - | Chr7:56653824-56653843 | MsG0780039002.01.T01:CDS | 45.0% | |
| TTGGCCAGAAGGAGGTAAAA+GGG | - | Chr7:56653177-56653196 | MsG0780039002.01.T01:intron | 45.0% | |
| ! | AGATTGAACGAAAGGTGCGA+AGG | - | Chr7:56653689-56653708 | MsG0780039002.01.T01:CDS | 45.0% |
| !! | CCTAAATCCCCTATTTCTGG+AGG | - | Chr7:56653020-56653039 | MsG0780039002.01.T01:CDS | 45.0% |
| AACGGCCTCAACCAGTTCAT+TGG | - | Chr7:56653327-56653346 | MsG0780039002.01.T01:intron | 50.0% | |
| CGTCCCACTTAATGATGTGG+AGG | - | Chr7:56653139-56653158 | MsG0780039002.01.T01:intron | 50.0% | |
| GCATTGGAGTCACAACCAAC+TGG | + | Chr7:56653082-56653101 | None:intergenic | 50.0% | |
| GGCAGGAAGTAGATCTGTTC+AGG | - | Chr7:56653782-56653801 | MsG0780039002.01.T01:CDS | 50.0% | |
| ! | CAGTCCCTTTTACCTCCTTC+TGG | + | Chr7:56653184-56653203 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 56652999 | 56653857 | 56652999 | ID=MsG0780039002.01;Name=MsG0780039002.01 |
| Chr7 | mRNA | 56652999 | 56653857 | 56652999 | ID=MsG0780039002.01.T01;Parent=MsG0780039002.01;Name=MsG0780039002.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|0.66|3|0|110 |
| Chr7 | exon | 56653666 | 56653857 | 56653666 | ID=MsG0780039002.01.T01:exon:8104;Parent=MsG0780039002.01.T01 |
| Chr7 | exon | 56653508 | 56653577 | 56653508 | ID=MsG0780039002.01.T01:exon:8103;Parent=MsG0780039002.01.T01 |
| Chr7 | exon | 56652999 | 56653069 | 56652999 | ID=MsG0780039002.01.T01:exon:8102;Parent=MsG0780039002.01.T01 |
| Chr7 | CDS | 56653666 | 56653857 | 56653666 | ID=MsG0780039002.01.T01:cds;Parent=MsG0780039002.01.T01 |
| Chr7 | CDS | 56653508 | 56653577 | 56653508 | ID=MsG0780039002.01.T01:cds;Parent=MsG0780039002.01.T01 |
| Chr7 | CDS | 56652999 | 56653069 | 56652999 | ID=MsG0780039002.01.T01:cds;Parent=MsG0780039002.01.T01 |
| Gene Sequence |
| Protein sequence |
