Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039247.01.T01 | XP_013449158.1 | 98 | 50 | 1 | 0 | 10 | 59 | 397 | 446 | 1.15E-23 | 102 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039247.01.T01 | P48621 | 70 | 60 | 17 | 1 | 1 | 59 | 394 | 453 | 3.23E-21 | 87.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039247.01.T01 | A0A072UBF9 | 98.000 | 50 | 1 | 0 | 10 | 59 | 397 | 446 | 5.48e-24 | 102 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011288.01 | MsG0780039247.01 | 0.801634 | 8.208050e-49 | 3.939267e-46 |
| MsG0280011473.01 | MsG0780039247.01 | 0.809569 | 1.773169e-50 | 1.044756e-47 |
| MsG0780039247.01 | MsG0780039248.01 | 0.871116 | 8.732814e-67 | 3.481222e-63 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039247.01.T01 | MTR_7g068780 | 98.000 | 50 | 1 | 0 | 10 | 59 | 397 | 446 | 1.39e-27 | 102 |
| MsG0780039247.01.T01 | MTR_4g015630 | 66.102 | 59 | 20 | 0 | 1 | 59 | 382 | 440 | 1.85e-22 | 88.2 |
| MsG0780039247.01.T01 | MTR_5g071170 | 60.465 | 43 | 17 | 0 | 10 | 52 | 343 | 385 | 1.03e-14 | 66.2 |
| MsG0780039247.01.T01 | MTR_3g464330 | 44.262 | 61 | 32 | 1 | 1 | 59 | 325 | 385 | 5.37e-13 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039247.01.T01 | AT5G05580 | 51.852 | 54 | 26 | 0 | 1 | 54 | 379 | 432 | 1.26e-16 | 72.0 |
| MsG0780039247.01.T01 | AT2G29980 | 50.000 | 52 | 26 | 0 | 1 | 52 | 324 | 375 | 2.59e-13 | 62.4 |
| MsG0780039247.01.T01 | AT3G11170 | 45.614 | 57 | 31 | 0 | 1 | 57 | 386 | 442 | 3.43e-13 | 62.0 |
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGAGGAGCTGATTTCTT+TGG | 0.246608 | 7:+60722908 | None:intergenic |
| ACCTTATTGGAGAATTTATA+AGG | 0.270680 | 7:-60722880 | MsG0780039247.01.T01:CDS |
| TCCTTATAAATTCTCCAATA+AGG | 0.310337 | 7:+60722879 | None:intergenic |
| GATCATTATGTTAGTGATAC+TGG | 0.339147 | 7:-60722845 | MsG0780039247.01.T01:CDS |
| TCTTAAATATCATTCTGATT+TGG | 0.361666 | 7:+60722778 | None:intergenic |
| CACTAATTTCAGAATCTGTT+TGG | 0.396613 | 7:+60722807 | None:intergenic |
| CAAACAGATTCTGAAATTAG+TGG | 0.428433 | 7:-60722806 | MsG0780039247.01.T01:CDS |
| GAGGCAGCAAGGCCTGTGTT+TGG | 0.442475 | 7:-60722946 | MsG0780039247.01.T01:CDS |
| AATTCTCCAATAAGGTGAAA+AGG | 0.487740 | 7:+60722887 | None:intergenic |
| CCAATAAGGTGAAAAGGAAG+AGG | 0.544622 | 7:+60722893 | None:intergenic |
| GTTTGCAGACTGAGGCAGCA+AGG | 0.573397 | 7:-60722957 | None:intergenic |
| TTTGTATATTCTCCAAACAC+AGG | 0.610736 | 7:+60722934 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACCTTATTGGAGAATTTATA+AGG | - | Chr7:60722844-60722863 | MsG0780039247.01.T01:CDS | 25.0% |
| ! | CCAAATCAGAATGATATTTA+AGG | - | Chr7:60722946-60722965 | MsG0780039247.01.T01:CDS | 25.0% |
| ! | TCCTTATAAATTCTCCAATA+AGG | + | Chr7:60722848-60722867 | None:intergenic | 25.0% |
| AATTCTCCAATAAGGTGAAA+AGG | + | Chr7:60722840-60722859 | None:intergenic | 30.0% | |
| CACTAATTTCAGAATCTGTT+TGG | + | Chr7:60722920-60722939 | None:intergenic | 30.0% | |
| GATCATTATGTTAGTGATAC+TGG | - | Chr7:60722879-60722898 | MsG0780039247.01.T01:CDS | 30.0% | |
| TTTGTATATTCTCCAAACAC+AGG | + | Chr7:60722793-60722812 | None:intergenic | 30.0% | |
| !! | CAAACAGATTCTGAAATTAG+TGG | - | Chr7:60722918-60722937 | MsG0780039247.01.T01:CDS | 30.0% |
| CCAATAAGGTGAAAAGGAAG+AGG | + | Chr7:60722834-60722853 | None:intergenic | 40.0% | |
| ! | CCTCTTCCTTTTCACCTTAT+TGG | - | Chr7:60722831-60722850 | MsG0780039247.01.T01:CDS | 40.0% |
| ! | GGAAGAGGAGCTGATTTCTT+TGG | + | Chr7:60722819-60722838 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 60722780 | 60722966 | 60722780 | ID=MsG0780039247.01;Name=MsG0780039247.01 |
| Chr7 | mRNA | 60722787 | 60722966 | 60722787 | ID=MsG0780039247.01.T01;Parent=MsG0780039247.01;Name=MsG0780039247.01.T01;_AED=0.47;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|59 |
| Chr7 | exon | 60722787 | 60722966 | 60722787 | ID=MsG0780039247.01.T01:exon:8537;Parent=MsG0780039247.01.T01 |
| Chr7 | CDS | 60722787 | 60722966 | 60722787 | ID=MsG0780039247.01.T01:cds;Parent=MsG0780039247.01.T01 |
| Chr7 | mRNA | 60722780 | 60722860 | 60722780 | ID=MsG0780039247.01.T02;Parent=MsG0780039247.01;Name=MsG0780039247.01.T02;_AED=0.49;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|26 |
| Chr7 | exon | 60722780 | 60722860 | 60722780 | ID=MsG0780039247.01.T02:exon:8538;Parent=MsG0780039247.01.T02 |
| Chr7 | CDS | 60722780 | 60722860 | 60722780 | ID=MsG0780039247.01.T02:cds;Parent=MsG0780039247.01.T02 |
| Gene Sequence |
| Protein sequence |
