Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039454.01.T01 | XP_003623656.2 | 74.921 | 315 | 12 | 2 | 1 | 248 | 1 | 315 | 6.71E-159 | 453 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480018211.01 | MsG0780039454.01 | 0.816432 | 5.548170e-52 | 3.930427e-49 |
| MsG0780039454.01 | MsG0780041341.01 | 0.802591 | 5.216909e-49 | 2.565503e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039454.01.T01 | MTR_7g074120 | 74.921 | 315 | 12 | 2 | 1 | 248 | 1 | 315 | 8.13e-163 | 453 |
| MsG0780039454.01.T01 | MTR_3g062840 | 41.694 | 307 | 106 | 5 | 8 | 248 | 12 | 311 | 2.74e-63 | 200 |
| MsG0780039454.01.T01 | MTR_5g075750 | 39.262 | 298 | 118 | 6 | 10 | 248 | 9 | 302 | 9.74e-59 | 188 |
| MsG0780039454.01.T01 | MTR_8g461170 | 37.857 | 280 | 115 | 7 | 17 | 238 | 6 | 284 | 1.67e-47 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039454.01.T01 | AT3G08600 | 41.219 | 279 | 94 | 3 | 39 | 247 | 37 | 315 | 6.33e-61 | 194 |
Find 54 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTAAGGAGTGGAAGTTTA+AGG | 0.141604 | 7:-64295851 | MsG0780039454.01.T01:CDS |
| TCCAATTTCAGCAATGTAAA+AGG | 0.214033 | 7:-64295514 | MsG0780039454.01.T01:CDS |
| AGGTTGCAGCATTAAGATTA+AGG | 0.267770 | 7:-64295866 | MsG0780039454.01.T01:CDS |
| TGCTTCTGGTGACAACATTT+TGG | 0.270480 | 7:-64295611 | MsG0780039454.01.T01:CDS |
| ATGGAGAGGGCTGCAGATTC+AGG | 0.278305 | 7:-64295258 | MsG0780039454.01.T01:CDS |
| GAACTTAATGTAAATGCTTC+TGG | 0.324955 | 7:-64295625 | MsG0780039454.01.T01:CDS |
| TTCAAATTTAACCGGAGTTA+AGG | 0.346389 | 7:-64295886 | MsG0780039454.01.T01:CDS |
| AGTGGAAGTTTAAGGAGAAA+AGG | 0.367884 | 7:-64295843 | MsG0780039454.01.T01:CDS |
| CAACTTCCTTCAAATTTAAC+CGG | 0.369770 | 7:-64295894 | MsG0780039454.01.T01:CDS |
| GTGGAAGTTTAAGGAGAAAA+GGG | 0.388779 | 7:-64295842 | MsG0780039454.01.T01:CDS |
| TGAATCACTTCCTCATGTTA+TGG | 0.397300 | 7:-64296024 | MsG0780039454.01.T01:CDS |
| GACTCCTTATGTTGAAAGAT+TGG | 0.397582 | 7:-64295775 | MsG0780039454.01.T01:CDS |
| AAATAAAGAAGCAAAGGCTT+TGG | 0.399626 | 7:-64295988 | MsG0780039454.01.T01:CDS |
| ACAATATGCTTACAAGGCTT+TGG | 0.408809 | 7:-64295952 | MsG0780039454.01.T01:CDS |
| AGCATCATTGTGCTTGGATA+AGG | 0.410802 | 7:+64296074 | None:intergenic |
| TTAACTCCGGTTAAATTTGA+AGG | 0.413147 | 7:+64295888 | None:intergenic |
| AAGCCTTGTAAGCATATTGT+TGG | 0.413943 | 7:+64295955 | None:intergenic |
| GAAGCATTTACATTAAGTTC+TGG | 0.421137 | 7:+64295628 | None:intergenic |
| CCATGTGGTGTAGGAGAAAC+TGG | 0.421235 | 7:+64295421 | None:intergenic |
| AACTGGTCCAGGTGCACCAT+TGG | 0.445508 | 7:+64295438 | None:intergenic |
| GAACTGTTGCAAAATGTGTT+TGG | 0.450124 | 7:-64295555 | MsG0780039454.01.T01:CDS |
| AAAACCAATCTTTCAACATA+AGG | 0.468023 | 7:+64295771 | None:intergenic |
| GCTTTGGTGAATCCGAAAAC+AGG | 0.475267 | 7:-64295936 | MsG0780039454.01.T01:CDS |
| GTCAAGTCCAATGGTGCACC+TGG | 0.476831 | 7:-64295445 | MsG0780039454.01.T01:CDS |
| TCTCCAACAATATGCTTACA+AGG | 0.482128 | 7:-64295958 | MsG0780039454.01.T01:CDS |
| TTCAGGAGAAACTCTTCAAA+TGG | 0.482960 | 7:-64295241 | MsG0780039454.01.T01:CDS |
| CTCACCTTTCCTTGACCATG+TGG | 0.501569 | 7:+64295406 | None:intergenic |
| CAAGAAGAAAATGCAAGGTA+TGG | 0.505816 | 7:-64295277 | MsG0780039454.01.T01:intron |
| ACTCTTCAAATGGCATCTGT+TGG | 0.515728 | 7:-64295231 | MsG0780039454.01.T01:CDS |
| TCTATAGTGGTCAAGTCCAA+TGG | 0.536015 | 7:-64295454 | MsG0780039454.01.T01:CDS |
| TTCTTCAAATAAAGAAGCAA+AGG | 0.547398 | 7:-64295994 | MsG0780039454.01.T01:CDS |
| TTTCCTTGACCATGTGGTGT+AGG | 0.552093 | 7:+64295412 | None:intergenic |
| AAAACAAGCATCATTGTGCT+TGG | 0.552555 | 7:+64296068 | None:intergenic |
| GTTCTTGTAACCGTTGCAGC+AGG | 0.557241 | 7:+64295195 | None:intergenic |
| AACAAAAGCACCTGCTGCAA+CGG | 0.558551 | 7:-64295205 | MsG0780039454.01.T01:CDS |
| GCATATTCATGTTCAAGTGT+TGG | 0.565606 | 7:+64295168 | None:intergenic |
| AGAAAATGCAAGGTATGGAG+AGG | 0.570714 | 7:-64295272 | MsG0780039454.01.T01:CDS |
| GTTGTTCAATCAGTACCTCA+TGG | 0.571134 | 7:-64295577 | MsG0780039454.01.T01:CDS |
| AATGAGTTTGAAATTCCCAC+TGG | 0.583157 | 7:-64295807 | MsG0780039454.01.T01:CDS |
| GGAGTCACAATTAGTCCAGT+GGG | 0.588995 | 7:+64295792 | None:intergenic |
| TGTGTTTGGTTTGATTTGCA+AGG | 0.591392 | 7:-64295541 | MsG0780039454.01.T01:CDS |
| GAAGATTGAGCCATAACATG+AGG | 0.597194 | 7:+64296014 | None:intergenic |
| TAATGCTGCAACCTTAACTC+CGG | 0.599525 | 7:+64295875 | None:intergenic |
| GGTGTAGGAGAAACTGGTCC+AGG | 0.612942 | 7:+64295427 | None:intergenic |
| TCTCCTACACCACATGGTCA+AGG | 0.616870 | 7:-64295415 | MsG0780039454.01.T01:CDS |
| GAGGTACTGATTGAACAACT+TGG | 0.621299 | 7:+64295581 | None:intergenic |
| AATTTCAGCAATGTAAAAGG+AGG | 0.628633 | 7:-64295511 | MsG0780039454.01.T01:CDS |
| AGGAGTCACAATTAGTCCAG+TGG | 0.634324 | 7:+64295791 | None:intergenic |
| TACACCACATGGTCAAGGAA+AGG | 0.637820 | 7:-64295410 | MsG0780039454.01.T01:intron |
| CCAGTTTCTCCTACACCACA+TGG | 0.637961 | 7:-64295421 | MsG0780039454.01.T01:CDS |
| AAACACAAGAAGAAAATGCA+AGG | 0.638593 | 7:-64295282 | MsG0780039454.01.T01:intron |
| GAAAATGCAAGGTATGGAGA+GGG | 0.647089 | 7:-64295271 | MsG0780039454.01.T01:CDS |
| ACATGTTCTACTTCACAACA+AGG | 0.688795 | 7:-64295484 | MsG0780039454.01.T01:CDS |
| GCAGCATTAAGATTAAGGAG+TGG | 0.710460 | 7:-64295861 | MsG0780039454.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAACCAATCTTTCAACATA+AGG | + | Chr7:64295476-64295495 | None:intergenic | 25.0% |
| ! | TTCTTCAAATAAAGAAGCAA+AGG | - | Chr7:64295250-64295269 | MsG0780039454.01.T01:CDS | 25.0% |
| !! | TAAAAAGTGTGGATTATTGT+TGG | - | Chr7:64295872-64295891 | MsG0780039454.01.T01:CDS | 25.0% |
| AAACACAAGAAGAAAATGCA+AGG | - | Chr7:64295962-64295981 | MsG0780039454.01.T01:CDS | 30.0% | |
| AAATAAAGAAGCAAAGGCTT+TGG | - | Chr7:64295256-64295275 | MsG0780039454.01.T01:CDS | 30.0% | |
| AAGAAGAGTAGTAAAAAGTG+TGG | - | Chr7:64295861-64295880 | MsG0780039454.01.T01:CDS | 30.0% | |
| AAGTAAGTGTAATTAGGCAA+AGG | + | Chr7:64295538-64295557 | None:intergenic | 30.0% | |
| AATTTCAGCAATGTAAAAGG+AGG | - | Chr7:64295733-64295752 | MsG0780039454.01.T01:intron | 30.0% | |
| CAACTTCCTTCAAATTTAAC+CGG | - | Chr7:64295350-64295369 | MsG0780039454.01.T01:intron | 30.0% | |
| GAACTTAATGTAAATGCTTC+TGG | - | Chr7:64295619-64295638 | MsG0780039454.01.T01:CDS | 30.0% | |
| TCCAATTTCAGCAATGTAAA+AGG | - | Chr7:64295730-64295749 | MsG0780039454.01.T01:intron | 30.0% | |
| TTAACTCCGGTTAAATTTGA+AGG | + | Chr7:64295359-64295378 | None:intergenic | 30.0% | |
| TTCAAATTTAACCGGAGTTA+AGG | - | Chr7:64295358-64295377 | MsG0780039454.01.T01:intron | 30.0% | |
| TTTGCCTAATTACACTTACT+TGG | - | Chr7:64295537-64295556 | MsG0780039454.01.T01:CDS | 30.0% | |
| ! | GAAGCATTTACATTAAGTTC+TGG | + | Chr7:64295619-64295638 | None:intergenic | 30.0% |
| ! | TCCTTTTACATTGCTGAAAT+TGG | + | Chr7:64295734-64295753 | None:intergenic | 30.0% |
| ! | TTGTAAACAGTACCTGTTTT+CGG | + | Chr7:64295323-64295342 | None:intergenic | 30.0% |
| !! | ATTATTGTTGGTTCTGTTGT+TGG | - | Chr7:64295884-64295903 | MsG0780039454.01.T01:CDS | 30.0% |
| !! | GGTTTTGTGTATCAAAATCT+AGG | - | Chr7:64295490-64295509 | MsG0780039454.01.T01:CDS | 30.0% |
| !! | TTATTGTTGGTTCTGTTGTT+GGG | - | Chr7:64295885-64295904 | MsG0780039454.01.T01:CDS | 30.0% |
| !!! | ACAAGGTCATTTTTCTATAG+TGG | - | Chr7:64295777-64295796 | MsG0780039454.01.T01:CDS | 30.0% |
| AAAACAAGCATCATTGTGCT+TGG | + | Chr7:64295179-64295198 | None:intergenic | 35.0% | |
| ACAATATGCTTACAAGGCTT+TGG | - | Chr7:64295292-64295311 | MsG0780039454.01.T01:intron | 35.0% | |
| ACATGTTCTACTTCACAACA+AGG | - | Chr7:64295760-64295779 | MsG0780039454.01.T01:intron | 35.0% | |
| AGGTTGCAGCATTAAGATTA+AGG | - | Chr7:64295378-64295397 | MsG0780039454.01.T01:intron | 35.0% | |
| AGTGGAAGTTTAAGGAGAAA+AGG | - | Chr7:64295401-64295420 | MsG0780039454.01.T01:intron | 35.0% | |
| CAAGAAGAAAATGCAAGGTA+TGG | - | Chr7:64295967-64295986 | MsG0780039454.01.T01:CDS | 35.0% | |
| GAACTGTTGCAAAATGTGTT+TGG | - | Chr7:64295689-64295708 | MsG0780039454.01.T01:intron | 35.0% | |
| GACTCCTTATGTTGAAAGAT+TGG | - | Chr7:64295469-64295488 | MsG0780039454.01.T01:CDS | 35.0% | |
| GATTAAGGAGTGGAAGTTTA+AGG | - | Chr7:64295393-64295412 | MsG0780039454.01.T01:intron | 35.0% | |
| GCATATTCATGTTCAAGTGT+TGG | + | Chr7:64296079-64296098 | None:intergenic | 35.0% | |
| GTGGAAGTTTAAGGAGAAAA+GGG | - | Chr7:64295402-64295421 | MsG0780039454.01.T01:intron | 35.0% | |
| TAAGCTAATAGTCCCAAAAC+CGG | + | Chr7:64295565-64295584 | None:intergenic | 35.0% | |
| TAATTACACTTACTTGGCAC+CGG | - | Chr7:64295543-64295562 | MsG0780039454.01.T01:CDS | 35.0% | |
| TCTCCAACAATATGCTTACA+AGG | - | Chr7:64295286-64295305 | MsG0780039454.01.T01:intron | 35.0% | |
| TGAATCACTTCCTCATGTTA+TGG | - | Chr7:64295220-64295239 | MsG0780039454.01.T01:CDS | 35.0% | |
| TTCAGGAGAAACTCTTCAAA+TGG | - | Chr7:64296003-64296022 | MsG0780039454.01.T01:CDS | 35.0% | |
| ! | AAGCCTTGTAAGCATATTGT+TGG | + | Chr7:64295292-64295311 | None:intergenic | 35.0% |
| ! | AATGAGTTTGAAATTCCCAC+TGG | - | Chr7:64295437-64295456 | MsG0780039454.01.T01:CDS | 35.0% |
| !! | TATTGTTGGTTCTGTTGTTG+GGG | - | Chr7:64295886-64295905 | MsG0780039454.01.T01:CDS | 35.0% |
| !! | TGTGTTTGGTTTGATTTGCA+AGG | - | Chr7:64295703-64295722 | MsG0780039454.01.T01:intron | 35.0% |
| ACTCTTCAAATGGCATCTGT+TGG | - | Chr7:64296013-64296032 | MsG0780039454.01.T01:CDS | 40.0% | |
| AGAAAATGCAAGGTATGGAG+AGG | - | Chr7:64295972-64295991 | MsG0780039454.01.T01:CDS | 40.0% | |
| AGCATCATTGTGCTTGGATA+AGG | + | Chr7:64295173-64295192 | None:intergenic | 40.0% | |
| GAAAATGCAAGGTATGGAGA+GGG | - | Chr7:64295973-64295992 | MsG0780039454.01.T01:CDS | 40.0% | |
| GAAGATTGAGCCATAACATG+AGG | + | Chr7:64295233-64295252 | None:intergenic | 40.0% | |
| GCAGCATTAAGATTAAGGAG+TGG | - | Chr7:64295383-64295402 | MsG0780039454.01.T01:intron | 40.0% | |
| GGTGCCAAGTAAGTGTAATT+AGG | + | Chr7:64295544-64295563 | None:intergenic | 40.0% | |
| GTTGTTCAATCAGTACCTCA+TGG | - | Chr7:64295667-64295686 | MsG0780039454.01.T01:intron | 40.0% | |
| TAATGCTGCAACCTTAACTC+CGG | + | Chr7:64295372-64295391 | None:intergenic | 40.0% | |
| TCTATAGTGGTCAAGTCCAA+TGG | - | Chr7:64295790-64295809 | MsG0780039454.01.T01:CDS | 40.0% | |
| ! | CATTTTGCAACAGTTCCATG+AGG | + | Chr7:64295685-64295704 | None:intergenic | 40.0% |
| ! | TGCTTCTGGTGACAACATTT+TGG | - | Chr7:64295633-64295652 | MsG0780039454.01.T01:CDS | 40.0% |
| ! | TGTTGGGGGATTGTTATTGT+TGG | - | Chr7:64295901-64295920 | MsG0780039454.01.T01:CDS | 40.0% |
| !! | ATTGTTGGTTCTGTTGTTGG+GGG | - | Chr7:64295887-64295906 | MsG0780039454.01.T01:CDS | 40.0% |
| !! | GAGGTACTGATTGAACAACT+TGG | + | Chr7:64295666-64295685 | None:intergenic | 40.0% |
| AGGAGTCACAATTAGTCCAG+TGG | + | Chr7:64295456-64295475 | None:intergenic | 45.0% | |
| GCTTTGGTGAATCCGAAAAC+AGG | - | Chr7:64295308-64295327 | MsG0780039454.01.T01:intron | 45.0% | |
| GGAGTCACAATTAGTCCAGT+GGG | + | Chr7:64295455-64295474 | None:intergenic | 45.0% | |
| TACACCACATGGTCAAGGAA+AGG | - | Chr7:64295834-64295853 | MsG0780039454.01.T01:CDS | 45.0% | |
| TTTCCTTGACCATGTGGTGT+AGG | + | Chr7:64295835-64295854 | None:intergenic | 45.0% | |
| !! | AACAAAAGCACCTGCTGCAA+CGG | - | Chr7:64296039-64296058 | MsG0780039454.01.T01:CDS | 45.0% |
| !!! | ACTTACTTGGCACCGGTTTT+GGG | - | Chr7:64295550-64295569 | MsG0780039454.01.T01:CDS | 45.0% |
| CCAGTTTCTCCTACACCACA+TGG | - | Chr7:64295823-64295842 | MsG0780039454.01.T01:CDS | 50.0% | |
| CCATGTGGTGTAGGAGAAAC+TGG | + | Chr7:64295826-64295845 | None:intergenic | 50.0% | |
| CTCACCTTTCCTTGACCATG+TGG | + | Chr7:64295841-64295860 | None:intergenic | 50.0% | |
| GTTCTTGTAACCGTTGCAGC+AGG | + | Chr7:64296052-64296071 | None:intergenic | 50.0% | |
| TCTCCTACACCACATGGTCA+AGG | - | Chr7:64295829-64295848 | MsG0780039454.01.T01:CDS | 50.0% | |
| !!! | CACTTACTTGGCACCGGTTT+TGG | - | Chr7:64295549-64295568 | MsG0780039454.01.T01:CDS | 50.0% |
| AACTGGTCCAGGTGCACCAT+TGG | + | Chr7:64295809-64295828 | None:intergenic | 55.0% | |
| ATGGAGAGGGCTGCAGATTC+AGG | - | Chr7:64295986-64296005 | MsG0780039454.01.T01:CDS | 55.0% | |
| GGTGTAGGAGAAACTGGTCC+AGG | + | Chr7:64295820-64295839 | None:intergenic | 55.0% | |
| GTCAAGTCCAATGGTGCACC+TGG | - | Chr7:64295799-64295818 | MsG0780039454.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 64295161 | 64296105 | 64295161 | ID=MsG0780039454.01;Name=MsG0780039454.01 |
| Chr7 | mRNA | 64295161 | 64296105 | 64295161 | ID=MsG0780039454.01.T01;Parent=MsG0780039454.01;Name=MsG0780039454.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|3|0|248 |
| Chr7 | exon | 64295769 | 64296105 | 64295769 | ID=MsG0780039454.01.T01:exon:9639;Parent=MsG0780039454.01.T01 |
| Chr7 | exon | 64295411 | 64295685 | 64295411 | ID=MsG0780039454.01.T01:exon:9638;Parent=MsG0780039454.01.T01 |
| Chr7 | exon | 64295161 | 64295295 | 64295161 | ID=MsG0780039454.01.T01:exon:9637;Parent=MsG0780039454.01.T01 |
| Chr7 | CDS | 64295769 | 64296105 | 64295769 | ID=MsG0780039454.01.T01:cds;Parent=MsG0780039454.01.T01 |
| Chr7 | CDS | 64295411 | 64295685 | 64295411 | ID=MsG0780039454.01.T01:cds;Parent=MsG0780039454.01.T01 |
| Chr7 | CDS | 64295161 | 64295295 | 64295161 | ID=MsG0780039454.01.T01:cds;Parent=MsG0780039454.01.T01 |
| Gene Sequence |
| Protein sequence |
