Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041341.01.T01 | XP_003625940.1 | 99.444 | 360 | 2 | 0 | 1 | 360 | 1 | 360 | 0 | 747 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041341.01.T01 | Q8VYF4 | 72.376 | 362 | 93 | 3 | 1 | 356 | 1 | 361 | 0 | 531 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041341.01.T01 | G7KSQ7 | 99.444 | 360 | 2 | 0 | 1 | 360 | 1 | 360 | 0.0 | 747 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780039454.01 | MsG0780041341.01 | 0.802591 | 5.216909e-49 | 2.565503e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041341.01.T01 | MTR_7g109210 | 99.444 | 360 | 2 | 0 | 1 | 360 | 1 | 360 | 0.0 | 747 |
| MsG0780041341.01.T01 | MTR_5g036580 | 60.504 | 357 | 124 | 6 | 15 | 354 | 6 | 362 | 1.85e-152 | 435 |
| MsG0780041341.01.T01 | MTR_6g008900 | 61.783 | 314 | 107 | 4 | 51 | 351 | 47 | 360 | 4.89e-147 | 420 |
| MsG0780041341.01.T01 | MTR_4g071620 | 61.783 | 314 | 107 | 4 | 51 | 351 | 47 | 360 | 4.89e-147 | 420 |
| MsG0780041341.01.T01 | MTR_3g092310 | 62.215 | 307 | 106 | 3 | 51 | 353 | 34 | 334 | 7.59e-146 | 416 |
| MsG0780041341.01.T01 | MTR_4g118030 | 54.360 | 344 | 145 | 5 | 17 | 357 | 10 | 344 | 1.45e-136 | 393 |
| MsG0780041341.01.T01 | MTR_1g021855 | 59.105 | 313 | 115 | 4 | 51 | 350 | 46 | 358 | 2.61e-136 | 393 |
| MsG0780041341.01.T01 | MTR_7g094230 | 58.086 | 303 | 115 | 2 | 51 | 351 | 51 | 343 | 6.90e-134 | 386 |
| MsG0780041341.01.T01 | MTR_8g078240 | 59.425 | 313 | 117 | 5 | 51 | 354 | 29 | 340 | 7.21e-133 | 383 |
| MsG0780041341.01.T01 | MTR_5g019220 | 57.843 | 306 | 122 | 2 | 52 | 350 | 53 | 358 | 2.12e-132 | 383 |
| MsG0780041341.01.T01 | MTR_3g111600 | 64.338 | 272 | 94 | 2 | 82 | 350 | 1 | 272 | 6.82e-132 | 378 |
| MsG0780041341.01.T01 | MTR_1g089820 | 59.136 | 301 | 117 | 2 | 54 | 352 | 51 | 347 | 1.69e-128 | 372 |
| MsG0780041341.01.T01 | MTR_2g461670 | 32.065 | 184 | 118 | 4 | 159 | 341 | 395 | 572 | 7.78e-25 | 106 |
| MsG0780041341.01.T01 | MTR_2g015550 | 34.359 | 195 | 119 | 5 | 159 | 353 | 339 | 524 | 8.85e-25 | 105 |
| MsG0780041341.01.T01 | MTR_4g109890 | 33.152 | 184 | 116 | 4 | 159 | 341 | 340 | 517 | 1.06e-24 | 105 |
| MsG0780041341.01.T01 | MTR_3g107930 | 26.756 | 299 | 176 | 6 | 75 | 337 | 351 | 642 | 3.27e-24 | 104 |
| MsG0780041341.01.T01 | MTR_3g107930 | 26.756 | 299 | 176 | 6 | 75 | 337 | 274 | 565 | 3.72e-24 | 103 |
| MsG0780041341.01.T01 | MTR_8g092000 | 33.140 | 172 | 110 | 4 | 159 | 330 | 384 | 550 | 9.40e-24 | 102 |
| MsG0780041341.01.T01 | MTR_2g027740 | 31.977 | 172 | 112 | 4 | 159 | 330 | 471 | 637 | 7.13e-22 | 97.4 |
| MsG0780041341.01.T01 | MTR_7g055600 | 28.436 | 211 | 140 | 6 | 136 | 341 | 211 | 415 | 1.58e-20 | 92.8 |
| MsG0780041341.01.T01 | MTR_7g057530 | 28.436 | 211 | 140 | 6 | 136 | 341 | 211 | 415 | 1.58e-20 | 92.8 |
| MsG0780041341.01.T01 | MTR_6g016380 | 31.395 | 172 | 113 | 4 | 159 | 330 | 359 | 525 | 2.24e-20 | 92.8 |
| MsG0780041341.01.T01 | MTR_7g075840 | 29.231 | 195 | 130 | 4 | 159 | 353 | 468 | 654 | 8.53e-20 | 91.3 |
| MsG0780041341.01.T01 | MTR_7g012370 | 30.726 | 179 | 112 | 6 | 159 | 330 | 334 | 507 | 1.02e-19 | 90.9 |
| MsG0780041341.01.T01 | MTR_2g082650 | 30.457 | 197 | 122 | 9 | 159 | 348 | 209 | 397 | 1.57e-19 | 89.7 |
| MsG0780041341.01.T01 | MTR_4g133570 | 29.949 | 197 | 123 | 7 | 159 | 348 | 261 | 449 | 2.60e-19 | 89.4 |
| MsG0780041341.01.T01 | MTR_2g082650 | 30.457 | 197 | 122 | 9 | 159 | 348 | 338 | 526 | 4.17e-19 | 89.0 |
| MsG0780041341.01.T01 | MTR_8g033360 | 27.820 | 266 | 150 | 8 | 74 | 302 | 318 | 578 | 4.73e-19 | 89.0 |
| MsG0780041341.01.T01 | MTR_3g452360 | 27.315 | 216 | 130 | 7 | 131 | 330 | 147 | 351 | 2.46e-18 | 85.9 |
| MsG0780041341.01.T01 | MTR_3g452360 | 28.723 | 188 | 107 | 7 | 159 | 330 | 324 | 500 | 7.71e-18 | 85.1 |
| MsG0780041341.01.T01 | MTR_2g006790 | 29.231 | 195 | 105 | 6 | 159 | 353 | 339 | 500 | 3.44e-14 | 73.9 |
| MsG0780041341.01.T01 | MTR_6g013840 | 31.788 | 151 | 92 | 6 | 187 | 330 | 356 | 502 | 6.63e-14 | 73.2 |
| MsG0780041341.01.T01 | MTR_7g096160 | 26.286 | 175 | 121 | 4 | 159 | 330 | 455 | 624 | 1.07e-13 | 72.8 |
| MsG0780041341.01.T01 | MTR_7g074680 | 28.859 | 149 | 95 | 5 | 186 | 333 | 497 | 635 | 1.44e-12 | 69.3 |
| MsG0780041341.01.T01 | MTR_7g074680 | 28.859 | 149 | 95 | 5 | 186 | 333 | 400 | 538 | 1.50e-12 | 68.9 |
| MsG0780041341.01.T01 | MTR_7g074680 | 28.859 | 149 | 95 | 5 | 186 | 333 | 480 | 618 | 1.58e-12 | 68.9 |
| MsG0780041341.01.T01 | MTR_7g074680 | 28.859 | 149 | 95 | 5 | 186 | 333 | 460 | 598 | 1.70e-12 | 68.9 |
| MsG0780041341.01.T01 | MTR_7g074680 | 28.859 | 149 | 95 | 5 | 186 | 333 | 460 | 598 | 1.72e-12 | 68.9 |
| MsG0780041341.01.T01 | MTR_7g074680 | 28.859 | 149 | 95 | 5 | 186 | 333 | 440 | 578 | 1.81e-12 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041341.01.T01 | AT3G62660 | 72.376 | 362 | 93 | 3 | 1 | 356 | 1 | 361 | 0.0 | 531 |
| MsG0780041341.01.T01 | AT1G02720 | 68.347 | 357 | 105 | 5 | 4 | 353 | 4 | 359 | 5.43e-179 | 501 |
| MsG0780041341.01.T01 | AT1G02720 | 68.347 | 357 | 105 | 5 | 4 | 353 | 4 | 359 | 5.43e-179 | 501 |
| MsG0780041341.01.T01 | AT4G02130 | 68.539 | 356 | 97 | 5 | 1 | 353 | 1 | 344 | 8.47e-176 | 492 |
| MsG0780041341.01.T01 | AT4G02130 | 68.539 | 356 | 97 | 5 | 1 | 353 | 1 | 344 | 8.47e-176 | 492 |
| MsG0780041341.01.T01 | AT4G02130 | 68.539 | 356 | 97 | 5 | 1 | 353 | 1 | 344 | 8.47e-176 | 492 |
| MsG0780041341.01.T01 | AT1G70090 | 67.302 | 315 | 92 | 4 | 51 | 354 | 51 | 365 | 2.30e-158 | 450 |
| MsG0780041341.01.T01 | AT1G70090 | 67.302 | 315 | 92 | 4 | 51 | 354 | 51 | 365 | 2.30e-158 | 450 |
| MsG0780041341.01.T01 | AT1G24170 | 64.577 | 319 | 98 | 4 | 51 | 354 | 50 | 368 | 7.14e-153 | 436 |
| MsG0780041341.01.T01 | AT3G28340 | 61.415 | 311 | 113 | 4 | 54 | 357 | 45 | 355 | 4.08e-146 | 417 |
| MsG0780041341.01.T01 | AT3G06260 | 57.012 | 328 | 131 | 2 | 31 | 355 | 26 | 346 | 2.18e-141 | 405 |
| MsG0780041341.01.T01 | AT1G13250 | 60.586 | 307 | 114 | 3 | 51 | 351 | 31 | 336 | 1.42e-132 | 382 |
| MsG0780041341.01.T01 | AT3G50760 | 57.878 | 311 | 124 | 5 | 51 | 354 | 25 | 335 | 3.54e-132 | 381 |
| MsG0780041341.01.T01 | AT1G19300 | 60.191 | 314 | 111 | 3 | 51 | 350 | 29 | 342 | 1.65e-130 | 377 |
| MsG0780041341.01.T01 | AT1G18580 | 32.673 | 202 | 129 | 6 | 134 | 333 | 315 | 511 | 2.14e-25 | 107 |
| MsG0780041341.01.T01 | AT5G47780 | 34.302 | 172 | 108 | 4 | 159 | 330 | 420 | 586 | 2.68e-25 | 107 |
| MsG0780041341.01.T01 | AT3G58790 | 33.505 | 194 | 115 | 8 | 159 | 345 | 204 | 390 | 6.62e-22 | 96.3 |
| MsG0780041341.01.T01 | AT4G38270 | 28.972 | 214 | 142 | 4 | 132 | 340 | 448 | 656 | 1.29e-21 | 96.7 |
| MsG0780041341.01.T01 | AT3G58790 | 33.505 | 194 | 115 | 8 | 159 | 345 | 336 | 522 | 1.38e-21 | 96.3 |
| MsG0780041341.01.T01 | AT4G38270 | 28.972 | 214 | 142 | 4 | 132 | 340 | 452 | 660 | 1.39e-21 | 96.7 |
| MsG0780041341.01.T01 | AT3G01040 | 27.572 | 243 | 152 | 9 | 106 | 330 | 233 | 469 | 2.40e-21 | 95.5 |
| MsG0780041341.01.T01 | AT2G20810 | 36.316 | 190 | 112 | 5 | 159 | 348 | 342 | 522 | 3.49e-21 | 95.1 |
| MsG0780041341.01.T01 | AT3G25140 | 28.910 | 211 | 139 | 6 | 136 | 341 | 336 | 540 | 8.85e-21 | 94.0 |
| MsG0780041341.01.T01 | AT5G15470 | 31.285 | 179 | 111 | 7 | 159 | 330 | 329 | 502 | 3.05e-20 | 92.4 |
| MsG0780041341.01.T01 | AT3G01040 | 30.168 | 179 | 113 | 6 | 159 | 330 | 329 | 502 | 4.51e-20 | 92.0 |
| MsG0780041341.01.T01 | AT3G01040 | 30.168 | 179 | 113 | 6 | 159 | 330 | 329 | 502 | 4.51e-20 | 92.0 |
| MsG0780041341.01.T01 | AT3G61130 | 28.796 | 191 | 131 | 4 | 159 | 349 | 477 | 662 | 3.54e-19 | 89.4 |
| MsG0780041341.01.T01 | AT5G54690 | 30.168 | 179 | 113 | 7 | 159 | 330 | 331 | 504 | 4.60e-19 | 89.0 |
| MsG0780041341.01.T01 | AT1G06780 | 29.714 | 175 | 115 | 4 | 159 | 330 | 403 | 572 | 3.87e-17 | 83.2 |
| MsG0780041341.01.T01 | AT1G06780 | 29.714 | 175 | 115 | 4 | 159 | 330 | 390 | 559 | 5.20e-17 | 82.8 |
| MsG0780041341.01.T01 | AT2G46480 | 29.381 | 194 | 126 | 4 | 159 | 349 | 344 | 529 | 6.03e-17 | 82.4 |
| MsG0780041341.01.T01 | AT2G46480 | 29.381 | 194 | 126 | 4 | 159 | 349 | 357 | 542 | 7.60e-17 | 82.4 |
| MsG0780041341.01.T01 | AT2G30575 | 29.143 | 175 | 116 | 4 | 159 | 330 | 411 | 580 | 1.17e-16 | 81.6 |
| MsG0780041341.01.T01 | AT3G02350 | 27.941 | 204 | 136 | 6 | 132 | 330 | 334 | 531 | 8.56e-15 | 75.9 |
| MsG0780041341.01.T01 | AT2G46480 | 26.804 | 194 | 106 | 6 | 159 | 349 | 344 | 504 | 6.55e-11 | 63.9 |
| MsG0780041341.01.T01 | AT2G46480 | 26.804 | 194 | 106 | 6 | 159 | 349 | 357 | 517 | 9.37e-11 | 63.5 |
Find 87 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGTGGAATACTGATTT+AGG | 0.164470 | 7:-89789581 | MsG0780041341.01.T01:CDS |
| TTGTCCTCTTGATGCACTTT+GGG | 0.299987 | 7:-89789141 | MsG0780041341.01.T01:CDS |
| AGTATTTCACGGCGGCGTTT+TGG | 0.306766 | 7:-89789511 | MsG0780041341.01.T01:CDS |
| CGGTGCATGTCATCCAAGTT+TGG | 0.347190 | 7:-89789945 | MsG0780041341.01.T01:CDS |
| CTTGTCCTCTTGATGCACTT+TGG | 0.359246 | 7:-89789142 | MsG0780041341.01.T01:CDS |
| ATGAATGATGGAAGATCTTA+TGG | 0.365766 | 7:+89790057 | None:intergenic |
| TGGGAACCCTTTGATTTGTA+TGG | 0.368755 | 7:-89789122 | MsG0780041341.01.T01:CDS |
| AGCTGTGGAATACTGATTTA+GGG | 0.377822 | 7:-89789580 | MsG0780041341.01.T01:CDS |
| AGGTGGAATCAACATGGTTT+AGG | 0.384694 | 7:-89789272 | MsG0780041341.01.T01:CDS |
| GTCCTGTTAGTTTGTTGCAT+TGG | 0.393792 | 7:-89789208 | MsG0780041341.01.T01:CDS |
| CTTTCCGCAGTTGAGATTCA+AGG | 0.399848 | 7:-89789774 | MsG0780041341.01.T01:CDS |
| GACCAATGCAACAAACTAAC+AGG | 0.418572 | 7:+89789206 | None:intergenic |
| GCACCGATTTCCCTTGAAAT+CGG | 0.420972 | 7:+89789962 | None:intergenic |
| CTAGTGAAGTGGAGGAAGAA+AGG | 0.432774 | 7:-89789413 | MsG0780041341.01.T01:CDS |
| AATGAGATGAATGGAGGGAG+GGG | 0.438795 | 7:+89790092 | None:intergenic |
| AAAGCTTGTTACTTTAACAC+TGG | 0.442886 | 7:-89789452 | MsG0780041341.01.T01:CDS |
| GTTAGTTTGTTGCATTGGTC+TGG | 0.452227 | 7:-89789203 | MsG0780041341.01.T01:CDS |
| GAAGATCTTATGGCTAAAGC+TGG | 0.454418 | 7:+89790067 | None:intergenic |
| ATCAATGCGAACCGATTTCA+AGG | 0.458921 | 7:-89789973 | MsG0780041341.01.T01:CDS |
| TGAAATCGGTTCGCATTGAT+CGG | 0.463182 | 7:+89789976 | None:intergenic |
| CTTGATGTCGAGTATCTTCG+TGG | 0.471187 | 7:-89789905 | MsG0780041341.01.T01:CDS |
| GATTTAGGGTTGAATACAAT+CGG | 0.479193 | 7:-89789566 | MsG0780041341.01.T01:CDS |
| TCTTGTTACAGATACGGATC+TGG | 0.484520 | 7:-89789816 | MsG0780041341.01.T01:CDS |
| ATCGCTACATGAACCAAACT+TGG | 0.489061 | 7:+89789932 | None:intergenic |
| TCACTTTCTTGTTACAGATA+CGG | 0.495941 | 7:-89789822 | MsG0780041341.01.T01:CDS |
| TAATTTCACAAAGTATTTCA+CGG | 0.496602 | 7:-89789522 | MsG0780041341.01.T01:CDS |
| TTCTCTACCACCGTTTCTGT+TGG | 0.497053 | 7:-89789327 | MsG0780041341.01.T01:CDS |
| TGGAAATTCAGAAGGTAGAA+AGG | 0.505528 | 7:-89789364 | MsG0780041341.01.T01:CDS |
| ACAAGAAGCGTGATAAAGAA+TGG | 0.507671 | 7:+89789862 | None:intergenic |
| GAACCGATTTCAAGGGAAAT+CGG | 0.509836 | 7:-89789965 | MsG0780041341.01.T01:CDS |
| GTGAGGGATATGATGAATGA+TGG | 0.509928 | 7:+89790045 | None:intergenic |
| GAAAGTAGAATCGGTAATCA+TGG | 0.516192 | 7:+89790023 | None:intergenic |
| TTGTTGCATTGGTCTGGTAG+TGG | 0.516801 | 7:-89789197 | MsG0780041341.01.T01:CDS |
| GATTCATCAAGTCTCCTCCA+TGG | 0.522549 | 7:+89789170 | None:intergenic |
| ATGAGATGAATGGAGGGAGG+GGG | 0.523168 | 7:+89790093 | None:intergenic |
| TCAAACGTCGTCGAAAACAG+CGG | 0.524874 | 7:+89789482 | None:intergenic |
| TTACTTTAACACTGGGGTGA+TGG | 0.524964 | 7:-89789444 | MsG0780041341.01.T01:CDS |
| TCAAGGTGTATTATTTCGAT+CGG | 0.527253 | 7:-89789757 | MsG0780041341.01.T01:CDS |
| TAGTGAAGTGGAGGAAGAAA+GGG | 0.529393 | 7:-89789412 | MsG0780041341.01.T01:CDS |
| GTAATGAGATGAATGGAGGG+AGG | 0.532268 | 7:+89790090 | None:intergenic |
| AAGCTTGTTACTTTAACACT+GGG | 0.538898 | 7:-89789451 | MsG0780041341.01.T01:CDS |
| GAGATGTGTGAAAGTAGAAT+CGG | 0.542455 | 7:+89790014 | None:intergenic |
| CTATGTCCATACAAATCAAA+GGG | 0.542655 | 7:+89789116 | None:intergenic |
| TGTGAAAAGAGTGATTTACT+TGG | 0.543378 | 7:-89789636 | MsG0780041341.01.T01:CDS |
| TAATGAGATGAATGGAGGGA+GGG | 0.549358 | 7:+89790091 | None:intergenic |
| GGAGAGGATTGAACGGTGGA+TGG | 0.549417 | 7:-89789384 | MsG0780041341.01.T01:CDS |
| AGTTGTAGAGATTTGCATCC+TGG | 0.553136 | 7:-89789230 | MsG0780041341.01.T01:CDS |
| CAAAGTGCATCAAGAGGACA+AGG | 0.556493 | 7:+89789143 | None:intergenic |
| TAATTTCTCGCGTAATTCAA+AGG | 0.560643 | 7:+89789683 | None:intergenic |
| TCTATGTCCATACAAATCAA+AGG | 0.562057 | 7:+89789115 | None:intergenic |
| CTGGGTAATGAGATGAATGG+AGG | 0.564006 | 7:+89790086 | None:intergenic |
| TAACACTGGGGTGATGGTGA+TGG | 0.570809 | 7:-89789438 | MsG0780041341.01.T01:CDS |
| GTAGAAAGGATCTACGAGCT+TGG | 0.583168 | 7:-89789350 | MsG0780041341.01.T01:CDS |
| AGATACGGATCTGGAAACAT+TGG | 0.587421 | 7:-89789807 | MsG0780041341.01.T01:CDS |
| GATGATGATTACGGTCATAA+CGG | 0.588188 | 7:+89790117 | None:intergenic |
| CAAACGTCGTCGAAAACAGC+GGG | 0.595541 | 7:+89789483 | None:intergenic |
| TGGGTAATGAGATGAATGGA+GGG | 0.597084 | 7:+89790087 | None:intergenic |
| CTTGAATCTCAACTGCGGAA+AGG | 0.601009 | 7:+89789775 | None:intergenic |
| AAGCTGGGTAATGAGATGAA+TGG | 0.605092 | 7:+89790083 | None:intergenic |
| TGCAACAAACTAACAGGACC+AGG | 0.605679 | 7:+89789212 | None:intergenic |
| TGGCGGCAATTGAGCATAGG+TGG | 0.606530 | 7:-89789289 | MsG0780041341.01.T01:CDS |
| TAGAATCGGTAATCATGGTG+AGG | 0.612464 | 7:+89790028 | None:intergenic |
| TGGAATCAACATGGTTTAGG+TGG | 0.612774 | 7:-89789269 | MsG0780041341.01.T01:CDS |
| AAGATCTTATGGCTAAAGCT+GGG | 0.614185 | 7:+89790068 | None:intergenic |
| TGGTGATGGATCTAGTGAAG+TGG | 0.619930 | 7:-89789424 | MsG0780041341.01.T01:CDS |
| AGAATCGGTAATCATGGTGA+GGG | 0.623637 | 7:+89790029 | None:intergenic |
| AAGACGATATTGCAAAGCTG+TGG | 0.625042 | 7:-89789595 | MsG0780041341.01.T01:CDS |
| ACGTGGCGGCAATTGAGCAT+AGG | 0.626736 | 7:-89789292 | MsG0780041341.01.T01:CDS |
| GTGGAGGAAGAAAGGGTACA+CGG | 0.632548 | 7:-89789405 | MsG0780041341.01.T01:CDS |
| GGAAGAAAGGGTACACGGAG+AGG | 0.637189 | 7:-89789400 | MsG0780041341.01.T01:CDS |
| GAGCATAGGTGGAATCAACA+TGG | 0.644441 | 7:-89789278 | MsG0780041341.01.T01:CDS |
| ACGGTGGATGGAAATTCAGA+AGG | 0.657697 | 7:-89789372 | MsG0780041341.01.T01:CDS |
| TGATGGATCTAGTGAAGTGG+AGG | 0.660738 | 7:-89789421 | MsG0780041341.01.T01:CDS |
| GGTTCCCAAAGTGCATCAAG+AGG | 0.662532 | 7:+89789137 | None:intergenic |
| TTAGGTGGTGACAATGTGAA+AGG | 0.677954 | 7:-89789254 | MsG0780041341.01.T01:CDS |
| TTACGCGAGAAATTATCTTG+CGG | 0.691015 | 7:-89789675 | MsG0780041341.01.T01:CDS |
| TCAATGCGAACCGATTTCAA+GGG | 0.694129 | 7:-89789972 | MsG0780041341.01.T01:CDS |
| TACACCTTGAATCTCAACTG+CGG | 0.694210 | 7:+89789770 | None:intergenic |
| GGTACACGGAGAGGATTGAA+CGG | 0.697280 | 7:-89789391 | MsG0780041341.01.T01:CDS |
| AGCTTGTTACTTTAACACTG+GGG | 0.709374 | 7:-89789450 | MsG0780041341.01.T01:CDS |
| AAATTAGCATGACAATATTG+CGG | 0.709527 | 7:+89789539 | None:intergenic |
| TCTTCGTGGTTCAATCGCTG+CGG | 0.713040 | 7:-89789891 | MsG0780041341.01.T01:CDS |
| GATGATTACGGTCATAACGG+CGG | 0.724011 | 7:+89790120 | None:intergenic |
| ACACGGAGAGGATTGAACGG+TGG | 0.746328 | 7:-89789388 | MsG0780041341.01.T01:CDS |
| CTGGTAGTGGAAAACCATGG+AGG | 0.747326 | 7:-89789184 | MsG0780041341.01.T01:CDS |
| GGTCTGGTAGTGGAAAACCA+TGG | 0.775483 | 7:-89789187 | MsG0780041341.01.T01:CDS |
| TTTCACAAAGTATTTCACGG+CGG | 0.819381 | 7:-89789519 | MsG0780041341.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTCACAAAGTATTTCA+CGG | - | Chr7:89789726-89789745 | MsG0780041341.01.T01:CDS | 20.0% |
| ! | AAATTAGCATGACAATATTG+CGG | + | Chr7:89789712-89789731 | None:intergenic | 25.0% |
| ! | AAGTGAAAAAAAACATTCTC+TGG | + | Chr7:89789412-89789431 | None:intergenic | 25.0% |
| !!! | TGTCGATTATCAAATTTTCA+CGG | - | Chr7:89789098-89789117 | MsG0780041341.01.T01:CDS | 25.0% |
| CTATGTCCATACAAATCAAA+GGG | + | Chr7:89790135-89790154 | None:intergenic | 30.0% | |
| TAATTTCTCGCGTAATTCAA+AGG | + | Chr7:89789568-89789587 | None:intergenic | 30.0% | |
| TCAAGGTGTATTATTTCGAT+CGG | - | Chr7:89789491-89789510 | MsG0780041341.01.T01:CDS | 30.0% | |
| TCACTTTCTTGTTACAGATA+CGG | - | Chr7:89789426-89789445 | MsG0780041341.01.T01:CDS | 30.0% | |
| TCTATGTCCATACAAATCAA+AGG | + | Chr7:89790136-89790155 | None:intergenic | 30.0% | |
| TGTGAAAAGAGTGATTTACT+TGG | - | Chr7:89789612-89789631 | MsG0780041341.01.T01:CDS | 30.0% | |
| ! | ATGAATGATGGAAGATCTTA+TGG | + | Chr7:89789194-89789213 | None:intergenic | 30.0% |
| ! | GATTTAGGGTTGAATACAAT+CGG | - | Chr7:89789682-89789701 | MsG0780041341.01.T01:CDS | 30.0% |
| !! | AAAGCTTGTTACTTTAACAC+TGG | - | Chr7:89789796-89789815 | MsG0780041341.01.T01:CDS | 30.0% |
| !! | AAGCTTGTTACTTTAACACT+GGG | - | Chr7:89789797-89789816 | MsG0780041341.01.T01:CDS | 30.0% |
| AAGATCTTATGGCTAAAGCT+GGG | + | Chr7:89789183-89789202 | None:intergenic | 35.0% | |
| GAAAGTAGAATCGGTAATCA+TGG | + | Chr7:89789228-89789247 | None:intergenic | 35.0% | |
| GAGATGTGTGAAAGTAGAAT+CGG | + | Chr7:89789237-89789256 | None:intergenic | 35.0% | |
| GATGATGATTACGGTCATAA+CGG | + | Chr7:89789134-89789153 | None:intergenic | 35.0% | |
| TGATCGGCATTACGAAAAAA+CGG | + | Chr7:89789259-89789278 | None:intergenic | 35.0% | |
| TGGAAATTCAGAAGGTAGAA+AGG | - | Chr7:89789884-89789903 | MsG0780041341.01.T01:CDS | 35.0% | |
| TTACGCGAGAAATTATCTTG+CGG | - | Chr7:89789573-89789592 | MsG0780041341.01.T01:CDS | 35.0% | |
| ! | ACAAGAAGCGTGATAAAGAA+TGG | + | Chr7:89789389-89789408 | None:intergenic | 35.0% |
| ! | TTTCACAAAGTATTTCACGG+CGG | - | Chr7:89789729-89789748 | MsG0780041341.01.T01:CDS | 35.0% |
| !! | AAGCTGTGGAATACTGATTT+AGG | - | Chr7:89789667-89789686 | MsG0780041341.01.T01:CDS | 35.0% |
| !! | AGCTGTGGAATACTGATTTA+GGG | - | Chr7:89789668-89789687 | MsG0780041341.01.T01:CDS | 35.0% |
| !! | AGCTTGTTACTTTAACACTG+GGG | - | Chr7:89789798-89789817 | MsG0780041341.01.T01:CDS | 35.0% |
| AAGACGATATTGCAAAGCTG+TGG | - | Chr7:89789653-89789672 | MsG0780041341.01.T01:CDS | 40.0% | |
| AAGCTGGGTAATGAGATGAA+TGG | + | Chr7:89789168-89789187 | None:intergenic | 40.0% | |
| AGAATCGGTAATCATGGTGA+GGG | + | Chr7:89789222-89789241 | None:intergenic | 40.0% | |
| AGATACGGATCTGGAAACAT+TGG | - | Chr7:89789441-89789460 | MsG0780041341.01.T01:CDS | 40.0% | |
| AGGTGGAATCAACATGGTTT+AGG | - | Chr7:89789976-89789995 | MsG0780041341.01.T01:CDS | 40.0% | |
| AGTTGTAGAGATTTGCATCC+TGG | - | Chr7:89790018-89790037 | MsG0780041341.01.T01:CDS | 40.0% | |
| ATCGCTACATGAACCAAACT+TGG | + | Chr7:89789319-89789338 | None:intergenic | 40.0% | |
| CCTGCAAAAACCAACAGAAA+CGG | + | Chr7:89789934-89789953 | None:intergenic | 40.0% | |
| GAAGATCTTATGGCTAAAGC+TGG | + | Chr7:89789184-89789203 | None:intergenic | 40.0% | |
| GACCAATGCAACAAACTAAC+AGG | + | Chr7:89790045-89790064 | None:intergenic | 40.0% | |
| GTGAGGGATATGATGAATGA+TGG | + | Chr7:89789206-89789225 | None:intergenic | 40.0% | |
| TAATGAGATGAATGGAGGGA+GGG | + | Chr7:89789160-89789179 | None:intergenic | 40.0% | |
| TACACCTTGAATCTCAACTG+CGG | + | Chr7:89789481-89789500 | None:intergenic | 40.0% | |
| TAGAATCGGTAATCATGGTG+AGG | + | Chr7:89789223-89789242 | None:intergenic | 40.0% | |
| TAGTGAAGTGGAGGAAGAAA+GGG | - | Chr7:89789836-89789855 | MsG0780041341.01.T01:CDS | 40.0% | |
| TCTTGTTACAGATACGGATC+TGG | - | Chr7:89789432-89789451 | MsG0780041341.01.T01:CDS | 40.0% | |
| TGGAATCAACATGGTTTAGG+TGG | - | Chr7:89789979-89789998 | MsG0780041341.01.T01:CDS | 40.0% | |
| TGGGTAATGAGATGAATGGA+GGG | + | Chr7:89789164-89789183 | None:intergenic | 40.0% | |
| TTACTTTAACACTGGGGTGA+TGG | - | Chr7:89789804-89789823 | MsG0780041341.01.T01:CDS | 40.0% | |
| TTAGGTGGTGACAATGTGAA+AGG | - | Chr7:89789994-89790013 | MsG0780041341.01.T01:CDS | 40.0% | |
| TTGTCCTCTTGATGCACTTT+GGG | - | Chr7:89790107-89790126 | MsG0780041341.01.T01:CDS | 40.0% | |
| ! | ATCAATGCGAACCGATTTCA+AGG | - | Chr7:89789275-89789294 | MsG0780041341.01.T01:CDS | 40.0% |
| ! | GAACCGATTTCAAGGGAAAT+CGG | - | Chr7:89789283-89789302 | MsG0780041341.01.T01:CDS | 40.0% |
| ! | TCAATGCGAACCGATTTCAA+GGG | - | Chr7:89789276-89789295 | MsG0780041341.01.T01:CDS | 40.0% |
| !! | GTCCTGTTAGTTTGTTGCAT+TGG | - | Chr7:89790040-89790059 | MsG0780041341.01.T01:CDS | 40.0% |
| !! | GTTAGTTTGTTGCATTGGTC+TGG | - | Chr7:89790045-89790064 | MsG0780041341.01.T01:CDS | 40.0% |
| !! | TGAAATCGGTTCGCATTGAT+CGG | + | Chr7:89789275-89789294 | None:intergenic | 40.0% |
| !! | TGGGAACCCTTTGATTTGTA+TGG | - | Chr7:89790126-89790145 | MsG0780041341.01.T01:CDS | 40.0% |
| AATGAGATGAATGGAGGGAG+GGG | + | Chr7:89789159-89789178 | None:intergenic | 45.0% | |
| ACGGTGGATGGAAATTCAGA+AGG | - | Chr7:89789876-89789895 | MsG0780041341.01.T01:CDS | 45.0% | |
| CAAAGTGCATCAAGAGGACA+AGG | + | Chr7:89790108-89790127 | None:intergenic | 45.0% | |
| CTAGTGAAGTGGAGGAAGAA+AGG | - | Chr7:89789835-89789854 | MsG0780041341.01.T01:CDS | 45.0% | |
| CTGGGTAATGAGATGAATGG+AGG | + | Chr7:89789165-89789184 | None:intergenic | 45.0% | |
| CTTGAATCTCAACTGCGGAA+AGG | + | Chr7:89789476-89789495 | None:intergenic | 45.0% | |
| CTTGATGTCGAGTATCTTCG+TGG | - | Chr7:89789343-89789362 | MsG0780041341.01.T01:CDS | 45.0% | |
| CTTGTCCTCTTGATGCACTT+TGG | - | Chr7:89790106-89790125 | MsG0780041341.01.T01:CDS | 45.0% | |
| CTTTCCGCAGTTGAGATTCA+AGG | - | Chr7:89789474-89789493 | MsG0780041341.01.T01:CDS | 45.0% | |
| GAGCATAGGTGGAATCAACA+TGG | - | Chr7:89789970-89789989 | MsG0780041341.01.T01:CDS | 45.0% | |
| GATGATTACGGTCATAACGG+CGG | + | Chr7:89789131-89789150 | None:intergenic | 45.0% | |
| GATTCATCAAGTCTCCTCCA+TGG | + | Chr7:89790081-89790100 | None:intergenic | 45.0% | |
| GCAAAAACCAACAGAAACGG+TGG | + | Chr7:89789931-89789950 | None:intergenic | 45.0% | |
| GTAATGAGATGAATGGAGGG+AGG | + | Chr7:89789161-89789180 | None:intergenic | 45.0% | |
| GTAGAAAGGATCTACGAGCT+TGG | - | Chr7:89789898-89789917 | MsG0780041341.01.T01:CDS | 45.0% | |
| TCAAACGTCGTCGAAAACAG+CGG | + | Chr7:89789769-89789788 | None:intergenic | 45.0% | |
| TGCAACAAACTAACAGGACC+AGG | + | Chr7:89790039-89790058 | None:intergenic | 45.0% | |
| TTCTCTACCACCGTTTCTGT+TGG | - | Chr7:89789921-89789940 | MsG0780041341.01.T01:CDS | 45.0% | |
| ! | TGATGGATCTAGTGAAGTGG+AGG | - | Chr7:89789827-89789846 | MsG0780041341.01.T01:CDS | 45.0% |
| ! | TTGTTGCATTGGTCTGGTAG+TGG | - | Chr7:89790051-89790070 | MsG0780041341.01.T01:CDS | 45.0% |
| !! | GCACCGATTTCCCTTGAAAT+CGG | + | Chr7:89789289-89789308 | None:intergenic | 45.0% |
| !! | TGGTGATGGATCTAGTGAAG+TGG | - | Chr7:89789824-89789843 | MsG0780041341.01.T01:CDS | 45.0% |
| !!! | CCGTTTCTGTTGGTTTTTGC+AGG | - | Chr7:89789931-89789950 | MsG0780041341.01.T01:CDS | 45.0% |
| ATGAGATGAATGGAGGGAGG+GGG | + | Chr7:89789158-89789177 | None:intergenic | 50.0% | |
| CAAACGTCGTCGAAAACAGC+GGG | + | Chr7:89789768-89789787 | None:intergenic | 50.0% | |
| CGGTGCATGTCATCCAAGTT+TGG | - | Chr7:89789303-89789322 | MsG0780041341.01.T01:CDS | 50.0% | |
| GGAGGGGGAGATGATGATTA+CGG | + | Chr7:89789143-89789162 | None:intergenic | 50.0% | |
| GGTACACGGAGAGGATTGAA+CGG | - | Chr7:89789857-89789876 | MsG0780041341.01.T01:CDS | 50.0% | |
| GGTTCCCAAAGTGCATCAAG+AGG | + | Chr7:89790114-89790133 | None:intergenic | 50.0% | |
| TCTTCGTGGTTCAATCGCTG+CGG | - | Chr7:89789357-89789376 | MsG0780041341.01.T01:CDS | 50.0% | |
| ! | AGTATTTCACGGCGGCGTTT+TGG | - | Chr7:89789737-89789756 | MsG0780041341.01.T01:CDS | 50.0% |
| ! | GTGGAGGAAGAAAGGGTACA+CGG | - | Chr7:89789843-89789862 | MsG0780041341.01.T01:CDS | 50.0% |
| ! | TAACACTGGGGTGATGGTGA+TGG | - | Chr7:89789810-89789829 | MsG0780041341.01.T01:CDS | 50.0% |
| !! | CTGGTAGTGGAAAACCATGG+AGG | - | Chr7:89790064-89790083 | MsG0780041341.01.T01:CDS | 50.0% |
| !! | GGTCTGGTAGTGGAAAACCA+TGG | - | Chr7:89790061-89790080 | MsG0780041341.01.T01:CDS | 50.0% |
| !!! | GTTGGTTTTTGCAGGACACG+TGG | - | Chr7:89789939-89789958 | MsG0780041341.01.T01:CDS | 50.0% |
| ACACGGAGAGGATTGAACGG+TGG | - | Chr7:89789860-89789879 | MsG0780041341.01.T01:CDS | 55.0% | |
| ACGTGGCGGCAATTGAGCAT+AGG | - | Chr7:89789956-89789975 | MsG0780041341.01.T01:CDS | 55.0% | |
| GGAGAGGATTGAACGGTGGA+TGG | - | Chr7:89789864-89789883 | MsG0780041341.01.T01:CDS | 55.0% | |
| TGGCGGCAATTGAGCATAGG+TGG | - | Chr7:89789959-89789978 | MsG0780041341.01.T01:CDS | 55.0% | |
| ! | GGAAGAAAGGGTACACGGAG+AGG | - | Chr7:89789848-89789867 | MsG0780041341.01.T01:CDS | 55.0% |
| ! | GGTTTTTGCAGGACACGTGG+CGG | - | Chr7:89789942-89789961 | MsG0780041341.01.T01:CDS | 55.0% |
| !! | TTTCACGGCGGCGTTTTGGT+CGG | - | Chr7:89789741-89789760 | MsG0780041341.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 89789094 | 89790176 | 89789094 | ID=MsG0780041341.01;Name=MsG0780041341.01 |
| Chr7 | mRNA | 89789094 | 89790176 | 89789094 | ID=MsG0780041341.01.T01;Parent=MsG0780041341.01;Name=MsG0780041341.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|360 |
| Chr7 | exon | 89789094 | 89790176 | 89789094 | ID=MsG0780041341.01.T01:exon:15751;Parent=MsG0780041341.01.T01 |
| Chr7 | CDS | 89789094 | 89790176 | 89789094 | ID=MsG0780041341.01.T01:cds;Parent=MsG0780041341.01.T01 |
| Gene Sequence |
| Protein sequence |
