Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039473.01.T01 | XP_003623819.1 | 91.018 | 167 | 15 | 0 | 1 | 167 | 1 | 167 | 2.63E-107 | 313 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039473.01.T01 | Q941L2 | 35.227 | 176 | 91 | 8 | 1 | 159 | 16 | 185 | 1.09E-17 | 79.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039473.01.T01 | G7L1G8 | 91.018 | 167 | 15 | 0 | 1 | 167 | 1 | 167 | 1.25e-107 | 313 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780039473.01 | MsG0780039607.01 | 0.842439 | 2.584786e-58 | 3.919698e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039473.01.T01 | MTR_7g076000 | 91.018 | 167 | 15 | 0 | 1 | 167 | 1 | 167 | 3.18e-111 | 313 |
| MsG0780039473.01.T01 | MTR_8g432400 | 53.333 | 180 | 65 | 4 | 1 | 164 | 1 | 177 | 8.43e-56 | 174 |
| MsG0780039473.01.T01 | MTR_8g432390 | 50.829 | 181 | 70 | 4 | 1 | 165 | 1 | 178 | 3.30e-52 | 164 |
| MsG0780039473.01.T01 | MTR_8g432350 | 48.619 | 181 | 74 | 4 | 1 | 165 | 1 | 178 | 3.59e-50 | 159 |
| MsG0780039473.01.T01 | MTR_8g432370 | 49.724 | 181 | 71 | 5 | 1 | 165 | 1 | 177 | 1.45e-49 | 158 |
| MsG0780039473.01.T01 | MTR_8g432450 | 37.222 | 180 | 81 | 7 | 2 | 157 | 5 | 176 | 1.34e-26 | 99.4 |
| MsG0780039473.01.T01 | MTR_8g432460 | 38.122 | 181 | 80 | 8 | 2 | 159 | 5 | 176 | 2.63e-26 | 99.0 |
| MsG0780039473.01.T01 | MTR_8g432470 | 37.363 | 182 | 81 | 8 | 2 | 159 | 5 | 177 | 2.31e-25 | 96.3 |
| MsG0780039473.01.T01 | MTR_8g432440 | 36.264 | 182 | 83 | 8 | 2 | 159 | 5 | 177 | 2.60e-25 | 96.3 |
| MsG0780039473.01.T01 | MTR_8g432420 | 36.264 | 182 | 83 | 8 | 2 | 159 | 5 | 177 | 2.60e-25 | 96.3 |
| MsG0780039473.01.T01 | MTR_3g064780 | 37.017 | 181 | 82 | 8 | 2 | 159 | 5 | 176 | 1.49e-23 | 93.2 |
| MsG0780039473.01.T01 | MTR_8g432490 | 35.196 | 179 | 84 | 7 | 2 | 157 | 5 | 174 | 1.92e-22 | 89.0 |
| MsG0780039473.01.T01 | MTR_8g432480 | 32.143 | 168 | 96 | 6 | 2 | 158 | 7 | 167 | 3.07e-16 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039473.01.T01 | AT3G61190 | 35.227 | 176 | 91 | 8 | 1 | 159 | 16 | 185 | 1.11e-18 | 79.3 |
| MsG0780039473.01.T01 | AT3G61190 | 35.227 | 176 | 91 | 8 | 1 | 159 | 16 | 185 | 1.11e-18 | 79.3 |
| MsG0780039473.01.T01 | AT2G45760 | 30.097 | 206 | 96 | 8 | 1 | 164 | 6 | 205 | 1.85e-17 | 76.3 |
Find 38 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATCTGACACTGGTATTC+TGG | 0.255440 | 7:+64600349 | None:intergenic |
| GCTGGTGATGATGGTTCTGC+TGG | 0.277820 | 7:-64600191 | MsG0780039473.01.T01:CDS |
| GGTTCTGCTGGGGTTGTAAC+AGG | 0.283757 | 7:-64600179 | MsG0780039473.01.T01:CDS |
| GTTACAGGTTATGGGACAAT+AGG | 0.323394 | 7:-64600295 | MsG0780039473.01.T01:CDS |
| TAATCCGGTGACCGGAATTC+CGG | 0.337399 | 7:-64600216 | MsG0780039473.01.T01:CDS |
| CGAATGTTGCTTAACCAAAC+TGG | 0.364343 | 7:+64600152 | None:intergenic |
| TTTGTATTTGCAAGATGATC+AGG | 0.377803 | 7:-64600330 | MsG0780039473.01.T01:CDS |
| GACAATAGGGTTAGAAGAAA+TGG | 0.402197 | 7:-64600281 | MsG0780039473.01.T01:CDS |
| ATTATATACCTTGCATTCAA+TGG | 0.414434 | 7:+64600425 | None:intergenic |
| CTGGTGATGATGGTTCTGCT+GGG | 0.446882 | 7:-64600190 | MsG0780039473.01.T01:CDS |
| TTGTAACAGGGATTCCAGTT+TGG | 0.454218 | 7:-64600166 | MsG0780039473.01.T01:CDS |
| TTACAGGTTATGGGACAATA+GGG | 0.459374 | 7:-64600294 | MsG0780039473.01.T01:CDS |
| ATCACCAGCCACCGGAATTC+CGG | 0.476382 | 7:+64600205 | None:intergenic |
| ATTCCGGTGGCTGGTGATGA+TGG | 0.489142 | 7:-64600200 | MsG0780039473.01.T01:CDS |
| AGTGATGGAATATTCTAATC+CGG | 0.494971 | 7:-64600231 | MsG0780039473.01.T01:CDS |
| GTGACCGGAATTCCGGTGGC+TGG | 0.497448 | 7:-64600209 | MsG0780039473.01.T01:CDS |
| ATGTGAAATGCAAAACATCA+TGG | 0.516454 | 7:-64600397 | MsG0780039473.01.T01:CDS |
| TTCAGTGACGGTAAAAGTGA+TGG | 0.538835 | 7:-64600246 | MsG0780039473.01.T01:CDS |
| GAATATTCTAATCCGGTGAC+CGG | 0.547449 | 7:-64600224 | MsG0780039473.01.T01:CDS |
| AATTCAGATGAATGTGGTGG+AGG | 0.549118 | 7:-64600488 | MsG0780039473.01.T01:CDS |
| GTTCTGCTGGGGTTGTAACA+GGG | 0.549517 | 7:-64600178 | MsG0780039473.01.T01:CDS |
| GAATATTAAAAGTGTTGGAA+TGG | 0.554841 | 7:-64600372 | MsG0780039473.01.T01:CDS |
| TTTGGTTAAGCAACATTCGA+AGG | 0.565286 | 7:-64600148 | MsG0780039473.01.T01:CDS |
| GCCACCGGAATTCCGGTCAC+CGG | 0.568046 | 7:+64600212 | None:intergenic |
| TTGGTTAAGCAACATTCGAA+GGG | 0.574468 | 7:-64600147 | MsG0780039473.01.T01:CDS |
| TGCAAATACAAATCTGACAC+TGG | 0.579315 | 7:+64600341 | None:intergenic |
| TGTTATCAATATTTCAGTGA+CGG | 0.580872 | 7:-64600258 | MsG0780039473.01.T01:CDS |
| ATGGAATGAGAAGCTTGTGA+TGG | 0.583840 | 7:-64600453 | MsG0780039473.01.T01:CDS |
| TCCGGTGACCGGAATTCCGG+TGG | 0.591349 | 7:-64600213 | MsG0780039473.01.T01:CDS |
| GATAATTCAGATGAATGTGG+TGG | 0.597269 | 7:-64600491 | MsG0780039473.01.T01:CDS |
| TGTGAAATGCAAAACATCAT+GGG | 0.605215 | 7:-64600396 | MsG0780039473.01.T01:CDS |
| TGGTGATGATGGTTCTGCTG+GGG | 0.605309 | 7:-64600189 | MsG0780039473.01.T01:CDS |
| GAACCATCATCACCAGCCAC+CGG | 0.627399 | 7:+64600197 | None:intergenic |
| AAAGATAATTCAGATGAATG+TGG | 0.633974 | 7:-64600494 | MsG0780039473.01.T01:CDS |
| GAATGAGAAGCTTGTGATGG+AGG | 0.639642 | 7:-64600450 | MsG0780039473.01.T01:CDS |
| TGGAGGTGCCATTGAATGCA+AGG | 0.641986 | 7:-64600433 | MsG0780039473.01.T01:CDS |
| GTGAAATGCAAAACATCATG+GGG | 0.674473 | 7:-64600395 | MsG0780039473.01.T01:CDS |
| TGAAATGCAAAACATCATGG+GGG | 0.759675 | 7:-64600394 | MsG0780039473.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAAATGCATTTTTCTTTA+TGG | + | Chr7:64600203-64600222 | None:intergenic | 20.0% |
| ! | AAAGATAATTCAGATGAATG+TGG | - | Chr7:64600265-64600284 | MsG0780039473.01.T01:CDS | 25.0% |
| ! | ATTATATACCTTGCATTCAA+TGG | + | Chr7:64600337-64600356 | None:intergenic | 25.0% |
| ! | TGTTATCAATATTTCAGTGA+CGG | - | Chr7:64600501-64600520 | MsG0780039473.01.T01:CDS | 25.0% |
| !! | TTCATCTGAATTATCTTTTG+TGG | + | Chr7:64600263-64600282 | None:intergenic | 25.0% |
| !!! | AATTTTTCAGTTACAGGTTA+TGG | - | Chr7:64600455-64600474 | MsG0780039473.01.T01:CDS | 25.0% |
| !!! | ATTTTTCAGTTACAGGTTAT+GGG | - | Chr7:64600456-64600475 | MsG0780039473.01.T01:CDS | 25.0% |
| !!! | GAATATTAAAAGTGTTGGAA+TGG | - | Chr7:64600387-64600406 | MsG0780039473.01.T01:CDS | 25.0% |
| !!! | TTATGGAGTTTTGATTCATA+TGG | + | Chr7:64600186-64600205 | None:intergenic | 25.0% |
| ATGTGAAATGCAAAACATCA+TGG | - | Chr7:64600362-64600381 | MsG0780039473.01.T01:CDS | 30.0% | |
| TGTGAAATGCAAAACATCAT+GGG | - | Chr7:64600363-64600382 | MsG0780039473.01.T01:CDS | 30.0% | |
| TTTGTATTTGCAAGATGATC+AGG | - | Chr7:64600429-64600448 | MsG0780039473.01.T01:CDS | 30.0% | |
| ! | AGTGATGGAATATTCTAATC+CGG | - | Chr7:64600528-64600547 | MsG0780039473.01.T01:CDS | 30.0% |
| GACAATAGGGTTAGAAGAAA+TGG | - | Chr7:64600478-64600497 | MsG0780039473.01.T01:CDS | 35.0% | |
| GATAATTCAGATGAATGTGG+TGG | - | Chr7:64600268-64600287 | MsG0780039473.01.T01:CDS | 35.0% | |
| GTGAAATGCAAAACATCATG+GGG | - | Chr7:64600364-64600383 | MsG0780039473.01.T01:CDS | 35.0% | |
| TGAAATGCAAAACATCATGG+GGG | - | Chr7:64600365-64600384 | MsG0780039473.01.T01:CDS | 35.0% | |
| TGCAAATACAAATCTGACAC+TGG | + | Chr7:64600421-64600440 | None:intergenic | 35.0% | |
| TTACAGGTTATGGGACAATA+GGG | - | Chr7:64600465-64600484 | MsG0780039473.01.T01:CDS | 35.0% | |
| TTGGTTAAGCAACATTCGAA+GGG | - | Chr7:64600612-64600631 | MsG0780039473.01.T01:CDS | 35.0% | |
| TTTGGTTAAGCAACATTCGA+AGG | - | Chr7:64600611-64600630 | MsG0780039473.01.T01:CDS | 35.0% | |
| ! | GAGGTTTTCTGCATTCAAAA+TGG | + | Chr7:64600164-64600183 | None:intergenic | 35.0% |
| ! | TGGGGGAATATTAAAAGTGT+TGG | - | Chr7:64600382-64600401 | MsG0780039473.01.T01:CDS | 35.0% |
| !! | AGGTGCAATTTTTCAGTTAC+AGG | - | Chr7:64600449-64600468 | MsG0780039473.01.T01:CDS | 35.0% |
| !!! | TGGAGTTTTGATTCATATGG+AGG | + | Chr7:64600183-64600202 | None:intergenic | 35.0% |
| AATTCAGATGAATGTGGTGG+AGG | - | Chr7:64600271-64600290 | MsG0780039473.01.T01:CDS | 40.0% | |
| ATGGAATGAGAAGCTTGTGA+TGG | - | Chr7:64600306-64600325 | MsG0780039473.01.T01:CDS | 40.0% | |
| CAAATCTGACACTGGTATTC+TGG | + | Chr7:64600413-64600432 | None:intergenic | 40.0% | |
| GAATATTCTAATCCGGTGAC+CGG | - | Chr7:64600535-64600554 | MsG0780039473.01.T01:CDS | 40.0% | |
| GTTACAGGTTATGGGACAAT+AGG | - | Chr7:64600464-64600483 | MsG0780039473.01.T01:CDS | 40.0% | |
| TTCAGTGACGGTAAAAGTGA+TGG | - | Chr7:64600513-64600532 | MsG0780039473.01.T01:CDS | 40.0% | |
| TTGTAACAGGGATTCCAGTT+TGG | - | Chr7:64600593-64600612 | MsG0780039473.01.T01:CDS | 40.0% | |
| ! | CGAATGTTGCTTAACCAAAC+TGG | + | Chr7:64600610-64600629 | None:intergenic | 40.0% |
| ! | GTGGAGGAAGCTTTCTTTTA+TGG | - | Chr7:64600287-64600306 | MsG0780039473.01.T01:CDS | 40.0% |
| GAATGAGAAGCTTGTGATGG+AGG | - | Chr7:64600309-64600328 | MsG0780039473.01.T01:CDS | 45.0% | |
| GTTCTGCTGGGGTTGTAACA+GGG | - | Chr7:64600581-64600600 | MsG0780039473.01.T01:CDS | 50.0% | |
| TAATCCGGTGACCGGAATTC+CGG | - | Chr7:64600543-64600562 | MsG0780039473.01.T01:CDS | 50.0% | |
| !! | CTGGTGATGATGGTTCTGCT+GGG | - | Chr7:64600569-64600588 | MsG0780039473.01.T01:CDS | 50.0% |
| !! | TGGAGGTGCCATTGAATGCA+AGG | - | Chr7:64600326-64600345 | MsG0780039473.01.T01:CDS | 50.0% |
| !! | TGGTGATGATGGTTCTGCTG+GGG | - | Chr7:64600570-64600589 | MsG0780039473.01.T01:CDS | 50.0% |
| ATCACCAGCCACCGGAATTC+CGG | + | Chr7:64600557-64600576 | None:intergenic | 55.0% | |
| GAACCATCATCACCAGCCAC+CGG | + | Chr7:64600565-64600584 | None:intergenic | 55.0% | |
| GGTTCTGCTGGGGTTGTAAC+AGG | - | Chr7:64600580-64600599 | MsG0780039473.01.T01:CDS | 55.0% | |
| ! | ATTCCGGTGGCTGGTGATGA+TGG | - | Chr7:64600559-64600578 | MsG0780039473.01.T01:CDS | 55.0% |
| !! | GCTGGTGATGATGGTTCTGC+TGG | - | Chr7:64600568-64600587 | MsG0780039473.01.T01:CDS | 55.0% |
| GCCACCGGAATTCCGGTCAC+CGG | + | Chr7:64600550-64600569 | None:intergenic | 65.0% | |
| GTGACCGGAATTCCGGTGGC+TGG | - | Chr7:64600550-64600569 | MsG0780039473.01.T01:CDS | 65.0% | |
| TCCGGTGACCGGAATTCCGG+TGG | - | Chr7:64600546-64600565 | MsG0780039473.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 64600139 | 64600642 | 64600139 | ID=MsG0780039473.01;Name=MsG0780039473.01 |
| Chr7 | mRNA | 64600139 | 64600642 | 64600139 | ID=MsG0780039473.01.T01;Parent=MsG0780039473.01;Name=MsG0780039473.01.T01;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|167 |
| Chr7 | exon | 64600139 | 64600642 | 64600139 | ID=MsG0780039473.01.T01:exon:17594;Parent=MsG0780039473.01.T01 |
| Chr7 | CDS | 64600139 | 64600642 | 64600139 | ID=MsG0780039473.01.T01:cds;Parent=MsG0780039473.01.T01 |
| Gene Sequence |
| Protein sequence |
