Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039607.01.T01 | XP_003623819.1 | 91.018 | 167 | 15 | 0 | 1 | 167 | 1 | 167 | 2.63E-107 | 313 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039607.01.T01 | Q941L2 | 35.227 | 176 | 91 | 8 | 1 | 159 | 16 | 185 | 1.09E-17 | 79.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039607.01.T01 | G7L1G8 | 91.018 | 167 | 15 | 0 | 1 | 167 | 1 | 167 | 1.25e-107 | 313 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021927.01 | MsG0780039607.01 | 0.803572 | 3.270377e-49 | 1.649213e-46 |
| MsG0480022575.01 | MsG0780039607.01 | 0.803916 | 2.774416e-49 | 1.411530e-46 |
| MsG0780039473.01 | MsG0780039607.01 | 0.842439 | 2.584786e-58 | 3.919698e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039607.01.T01 | MTR_7g076000 | 91.018 | 167 | 15 | 0 | 1 | 167 | 1 | 167 | 3.18e-111 | 313 |
| MsG0780039607.01.T01 | MTR_8g432400 | 53.333 | 180 | 65 | 4 | 1 | 164 | 1 | 177 | 8.43e-56 | 174 |
| MsG0780039607.01.T01 | MTR_8g432390 | 50.829 | 181 | 70 | 4 | 1 | 165 | 1 | 178 | 3.30e-52 | 164 |
| MsG0780039607.01.T01 | MTR_8g432350 | 48.619 | 181 | 74 | 4 | 1 | 165 | 1 | 178 | 3.59e-50 | 159 |
| MsG0780039607.01.T01 | MTR_8g432370 | 49.724 | 181 | 71 | 5 | 1 | 165 | 1 | 177 | 1.45e-49 | 158 |
| MsG0780039607.01.T01 | MTR_8g432450 | 37.222 | 180 | 81 | 7 | 2 | 157 | 5 | 176 | 1.34e-26 | 99.4 |
| MsG0780039607.01.T01 | MTR_8g432460 | 38.122 | 181 | 80 | 8 | 2 | 159 | 5 | 176 | 2.63e-26 | 99.0 |
| MsG0780039607.01.T01 | MTR_8g432470 | 37.363 | 182 | 81 | 8 | 2 | 159 | 5 | 177 | 2.31e-25 | 96.3 |
| MsG0780039607.01.T01 | MTR_8g432440 | 36.264 | 182 | 83 | 8 | 2 | 159 | 5 | 177 | 2.60e-25 | 96.3 |
| MsG0780039607.01.T01 | MTR_8g432420 | 36.264 | 182 | 83 | 8 | 2 | 159 | 5 | 177 | 2.60e-25 | 96.3 |
| MsG0780039607.01.T01 | MTR_3g064780 | 37.017 | 181 | 82 | 8 | 2 | 159 | 5 | 176 | 1.49e-23 | 93.2 |
| MsG0780039607.01.T01 | MTR_8g432490 | 35.196 | 179 | 84 | 7 | 2 | 157 | 5 | 174 | 1.92e-22 | 89.0 |
| MsG0780039607.01.T01 | MTR_8g432480 | 32.143 | 168 | 96 | 6 | 2 | 158 | 7 | 167 | 3.07e-16 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039607.01.T01 | AT3G61190 | 35.227 | 176 | 91 | 8 | 1 | 159 | 16 | 185 | 1.11e-18 | 79.3 |
| MsG0780039607.01.T01 | AT3G61190 | 35.227 | 176 | 91 | 8 | 1 | 159 | 16 | 185 | 1.11e-18 | 79.3 |
| MsG0780039607.01.T01 | AT2G45760 | 30.097 | 206 | 96 | 8 | 1 | 164 | 6 | 205 | 1.85e-17 | 76.3 |
Find 38 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATCTGACACTGGTATTC+TGG | 0.255440 | 7:-66658288 | None:intergenic |
| GCTGGTGATGATGGTTCTGC+TGG | 0.277820 | 7:+66658446 | MsG0780039607.01.T01:CDS |
| GGTTCTGCTGGGGTTGTAAC+AGG | 0.283757 | 7:+66658458 | MsG0780039607.01.T01:CDS |
| GTTACAGGTTATGGGACAAT+AGG | 0.323394 | 7:+66658342 | MsG0780039607.01.T01:CDS |
| TAATCCGGTGACCGGAATTC+CGG | 0.337399 | 7:+66658421 | MsG0780039607.01.T01:CDS |
| CGAATGTTGCTTAACCAAAC+TGG | 0.364343 | 7:-66658485 | None:intergenic |
| TTTGTATTTGCAAGATGATC+AGG | 0.377803 | 7:+66658307 | MsG0780039607.01.T01:CDS |
| GACAATAGGGTTAGAAGAAA+TGG | 0.402197 | 7:+66658356 | MsG0780039607.01.T01:CDS |
| ATTATATACCTTGCATTCAA+TGG | 0.414434 | 7:-66658212 | None:intergenic |
| CTGGTGATGATGGTTCTGCT+GGG | 0.446882 | 7:+66658447 | MsG0780039607.01.T01:CDS |
| TTGTAACAGGGATTCCAGTT+TGG | 0.454218 | 7:+66658471 | MsG0780039607.01.T01:CDS |
| TTACAGGTTATGGGACAATA+GGG | 0.459374 | 7:+66658343 | MsG0780039607.01.T01:CDS |
| ATCACCAGCCACCGGAATTC+CGG | 0.476382 | 7:-66658432 | None:intergenic |
| ATTCCGGTGGCTGGTGATGA+TGG | 0.489142 | 7:+66658437 | MsG0780039607.01.T01:CDS |
| AGTGATGGAATATTCTAATC+CGG | 0.494971 | 7:+66658406 | MsG0780039607.01.T01:CDS |
| GTGACCGGAATTCCGGTGGC+TGG | 0.497448 | 7:+66658428 | MsG0780039607.01.T01:CDS |
| ATGTGAAATGCAAAACATCA+TGG | 0.516454 | 7:+66658240 | MsG0780039607.01.T01:CDS |
| TTCAGTGACGGTAAAAGTGA+TGG | 0.538835 | 7:+66658391 | MsG0780039607.01.T01:CDS |
| GAATATTCTAATCCGGTGAC+CGG | 0.547449 | 7:+66658413 | MsG0780039607.01.T01:CDS |
| AATTCAGATGAATGTGGTGG+AGG | 0.549118 | 7:+66658149 | MsG0780039607.01.T01:CDS |
| GTTCTGCTGGGGTTGTAACA+GGG | 0.549517 | 7:+66658459 | MsG0780039607.01.T01:CDS |
| GAATATTAAAAGTGTTGGAA+TGG | 0.554841 | 7:+66658265 | MsG0780039607.01.T01:CDS |
| TTTGGTTAAGCAACATTCGA+AGG | 0.565286 | 7:+66658489 | MsG0780039607.01.T01:CDS |
| GCCACCGGAATTCCGGTCAC+CGG | 0.568046 | 7:-66658425 | None:intergenic |
| TTGGTTAAGCAACATTCGAA+GGG | 0.574468 | 7:+66658490 | MsG0780039607.01.T01:CDS |
| TGCAAATACAAATCTGACAC+TGG | 0.579315 | 7:-66658296 | None:intergenic |
| TGTTATCAATATTTCAGTGA+CGG | 0.580872 | 7:+66658379 | MsG0780039607.01.T01:CDS |
| ATGGAATGAGAAGCTTGTGA+TGG | 0.583840 | 7:+66658184 | MsG0780039607.01.T01:CDS |
| TCCGGTGACCGGAATTCCGG+TGG | 0.591349 | 7:+66658424 | MsG0780039607.01.T01:CDS |
| GATAATTCAGATGAATGTGG+TGG | 0.597269 | 7:+66658146 | MsG0780039607.01.T01:CDS |
| TGTGAAATGCAAAACATCAT+GGG | 0.605215 | 7:+66658241 | MsG0780039607.01.T01:CDS |
| TGGTGATGATGGTTCTGCTG+GGG | 0.605309 | 7:+66658448 | MsG0780039607.01.T01:CDS |
| GAACCATCATCACCAGCCAC+CGG | 0.627399 | 7:-66658440 | None:intergenic |
| AAAGATAATTCAGATGAATG+TGG | 0.633974 | 7:+66658143 | MsG0780039607.01.T01:CDS |
| GAATGAGAAGCTTGTGATGG+AGG | 0.639642 | 7:+66658187 | MsG0780039607.01.T01:CDS |
| TGGAGGTGCCATTGAATGCA+AGG | 0.641986 | 7:+66658204 | MsG0780039607.01.T01:CDS |
| GTGAAATGCAAAACATCATG+GGG | 0.674473 | 7:+66658242 | MsG0780039607.01.T01:CDS |
| TGAAATGCAAAACATCATGG+GGG | 0.759675 | 7:+66658243 | MsG0780039607.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAAATGCATTTTTCTTTA+TGG | - | Chr7:66658081-66658100 | None:intergenic | 20.0% |
| ! | AAAGATAATTCAGATGAATG+TGG | + | Chr7:66658143-66658162 | MsG0780039607.01.T01:CDS | 25.0% |
| ! | ATTATATACCTTGCATTCAA+TGG | - | Chr7:66658215-66658234 | None:intergenic | 25.0% |
| ! | TGTTATCAATATTTCAGTGA+CGG | + | Chr7:66658379-66658398 | MsG0780039607.01.T01:CDS | 25.0% |
| !! | TTCATCTGAATTATCTTTTG+TGG | - | Chr7:66658141-66658160 | None:intergenic | 25.0% |
| !!! | AATTTTTCAGTTACAGGTTA+TGG | + | Chr7:66658333-66658352 | MsG0780039607.01.T01:CDS | 25.0% |
| !!! | ATTTTTCAGTTACAGGTTAT+GGG | + | Chr7:66658334-66658353 | MsG0780039607.01.T01:CDS | 25.0% |
| !!! | GAATATTAAAAGTGTTGGAA+TGG | + | Chr7:66658265-66658284 | MsG0780039607.01.T01:CDS | 25.0% |
| !!! | TTATGGAGTTTTGATTCATA+TGG | - | Chr7:66658064-66658083 | None:intergenic | 25.0% |
| ATGTGAAATGCAAAACATCA+TGG | + | Chr7:66658240-66658259 | MsG0780039607.01.T01:CDS | 30.0% | |
| TGTGAAATGCAAAACATCAT+GGG | + | Chr7:66658241-66658260 | MsG0780039607.01.T01:CDS | 30.0% | |
| TTTGTATTTGCAAGATGATC+AGG | + | Chr7:66658307-66658326 | MsG0780039607.01.T01:CDS | 30.0% | |
| ! | AGTGATGGAATATTCTAATC+CGG | + | Chr7:66658406-66658425 | MsG0780039607.01.T01:CDS | 30.0% |
| GACAATAGGGTTAGAAGAAA+TGG | + | Chr7:66658356-66658375 | MsG0780039607.01.T01:CDS | 35.0% | |
| GATAATTCAGATGAATGTGG+TGG | + | Chr7:66658146-66658165 | MsG0780039607.01.T01:CDS | 35.0% | |
| GTGAAATGCAAAACATCATG+GGG | + | Chr7:66658242-66658261 | MsG0780039607.01.T01:CDS | 35.0% | |
| TGAAATGCAAAACATCATGG+GGG | + | Chr7:66658243-66658262 | MsG0780039607.01.T01:CDS | 35.0% | |
| TGCAAATACAAATCTGACAC+TGG | - | Chr7:66658299-66658318 | None:intergenic | 35.0% | |
| TTACAGGTTATGGGACAATA+GGG | + | Chr7:66658343-66658362 | MsG0780039607.01.T01:CDS | 35.0% | |
| TTGGTTAAGCAACATTCGAA+GGG | + | Chr7:66658490-66658509 | MsG0780039607.01.T01:CDS | 35.0% | |
| TTTGGTTAAGCAACATTCGA+AGG | + | Chr7:66658489-66658508 | MsG0780039607.01.T01:CDS | 35.0% | |
| ! | GAGGTTTTCTGCATTCAAAA+TGG | - | Chr7:66658042-66658061 | None:intergenic | 35.0% |
| ! | TGGGGGAATATTAAAAGTGT+TGG | + | Chr7:66658260-66658279 | MsG0780039607.01.T01:CDS | 35.0% |
| !! | AGGTGCAATTTTTCAGTTAC+AGG | + | Chr7:66658327-66658346 | MsG0780039607.01.T01:CDS | 35.0% |
| !!! | TGGAGTTTTGATTCATATGG+AGG | - | Chr7:66658061-66658080 | None:intergenic | 35.0% |
| AATTCAGATGAATGTGGTGG+AGG | + | Chr7:66658149-66658168 | MsG0780039607.01.T01:CDS | 40.0% | |
| ATGGAATGAGAAGCTTGTGA+TGG | + | Chr7:66658184-66658203 | MsG0780039607.01.T01:CDS | 40.0% | |
| CAAATCTGACACTGGTATTC+TGG | - | Chr7:66658291-66658310 | None:intergenic | 40.0% | |
| GAATATTCTAATCCGGTGAC+CGG | + | Chr7:66658413-66658432 | MsG0780039607.01.T01:CDS | 40.0% | |
| GTTACAGGTTATGGGACAAT+AGG | + | Chr7:66658342-66658361 | MsG0780039607.01.T01:CDS | 40.0% | |
| TTCAGTGACGGTAAAAGTGA+TGG | + | Chr7:66658391-66658410 | MsG0780039607.01.T01:CDS | 40.0% | |
| TTGTAACAGGGATTCCAGTT+TGG | + | Chr7:66658471-66658490 | MsG0780039607.01.T01:CDS | 40.0% | |
| ! | CGAATGTTGCTTAACCAAAC+TGG | - | Chr7:66658488-66658507 | None:intergenic | 40.0% |
| ! | GTGGAGGAAGCTTTCTTTTA+TGG | + | Chr7:66658165-66658184 | MsG0780039607.01.T01:CDS | 40.0% |
| GAATGAGAAGCTTGTGATGG+AGG | + | Chr7:66658187-66658206 | MsG0780039607.01.T01:CDS | 45.0% | |
| GTTCTGCTGGGGTTGTAACA+GGG | + | Chr7:66658459-66658478 | MsG0780039607.01.T01:CDS | 50.0% | |
| TAATCCGGTGACCGGAATTC+CGG | + | Chr7:66658421-66658440 | MsG0780039607.01.T01:CDS | 50.0% | |
| !! | CTGGTGATGATGGTTCTGCT+GGG | + | Chr7:66658447-66658466 | MsG0780039607.01.T01:CDS | 50.0% |
| !! | TGGAGGTGCCATTGAATGCA+AGG | + | Chr7:66658204-66658223 | MsG0780039607.01.T01:CDS | 50.0% |
| !! | TGGTGATGATGGTTCTGCTG+GGG | + | Chr7:66658448-66658467 | MsG0780039607.01.T01:CDS | 50.0% |
| ATCACCAGCCACCGGAATTC+CGG | - | Chr7:66658435-66658454 | None:intergenic | 55.0% | |
| GAACCATCATCACCAGCCAC+CGG | - | Chr7:66658443-66658462 | None:intergenic | 55.0% | |
| GGTTCTGCTGGGGTTGTAAC+AGG | + | Chr7:66658458-66658477 | MsG0780039607.01.T01:CDS | 55.0% | |
| ! | ATTCCGGTGGCTGGTGATGA+TGG | + | Chr7:66658437-66658456 | MsG0780039607.01.T01:CDS | 55.0% |
| !! | GCTGGTGATGATGGTTCTGC+TGG | + | Chr7:66658446-66658465 | MsG0780039607.01.T01:CDS | 55.0% |
| GCCACCGGAATTCCGGTCAC+CGG | - | Chr7:66658428-66658447 | None:intergenic | 65.0% | |
| GTGACCGGAATTCCGGTGGC+TGG | + | Chr7:66658428-66658447 | MsG0780039607.01.T01:CDS | 65.0% | |
| TCCGGTGACCGGAATTCCGG+TGG | + | Chr7:66658424-66658443 | MsG0780039607.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 66658017 | 66658520 | 66658017 | ID=MsG0780039607.01;Name=MsG0780039607.01 |
| Chr7 | mRNA | 66658017 | 66658520 | 66658017 | ID=MsG0780039607.01.T01;Parent=MsG0780039607.01;Name=MsG0780039607.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|167 |
| Chr7 | exon | 66658017 | 66658520 | 66658017 | ID=MsG0780039607.01.T01:exon:11673;Parent=MsG0780039607.01.T01 |
| Chr7 | CDS | 66658017 | 66658520 | 66658017 | ID=MsG0780039607.01.T01:cds;Parent=MsG0780039607.01.T01 |
| Gene Sequence |
| Protein sequence |
