AlfalfaGEDB | MsG0780039678.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039678.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039678.01.T02	AES80150.1	96.512	86	3	1	86	1	86	1.93E-51	166
MsG0780039678.01.T04	AES80150.1	96.825	63	2	1	63	24	86	1.13E-34	122
MsG0780039678.01.T03	AES80150.1	96.203	79	3	1	79	8	86	5.92E-46	152

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039678.01.T02	P16346	89.655	58	6	29	86	1	58	9.42E-32	108
MsG0780039678.01.T03	P16346	89.655	58	6	22	79	1	58	1.69E-31	107
MsG0780039678.01.T04	P16346	89.655	58	6	6	63	1	58	4.79E-31	105

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039678.01.T02	G7L3N0	96.512	86	3	1	86	1	86	9.24e-52	166
MsG0780039678.01.T03	G7L3N0	96.203	79	3	1	79	8	86	2.83e-46	152
MsG0780039678.01.T04	G7L3N0	96.825	63	2	1	63	24	86	5.39e-35	122

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010698.01	MsG0780039678.01	0.804309	2.297633e-49	1.180750e-46
MsG0380012460.01	MsG0780039678.01	0.810837	9.451341e-51	5.758334e-48
MsG0380014537.01	MsG0780039678.01	0.817201	3.729332e-52	2.698290e-49
MsG0580024781.01	MsG0780039678.01	0.810671	1.026503e-50	6.226491e-48
MsG0380015555.01	MsG0780039678.01	0.802288	6.022672e-49	2.939105e-46
MsG0480018163.01	MsG0780039678.01	0.805496	1.297852e-49	6.876465e-47
MsG0280007547.01	MsG0780039678.01	0.808895	2.474005e-50	1.431537e-47
MsG0280009748.01	MsG0780039678.01	0.801980	6.968673e-49	3.373843e-46
MsG0780038401.01	MsG0780039678.01	0.806218	9.151673e-50	4.939709e-47
MsG0780039678.01	MsG0880045937.01	0.818422	1.976975e-52	1.479286e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039678.01.T02	MTR_7g077180	96.512	86	3	0	1	86	1	86	2.34e-55	166
MsG0780039678.01.T02	MTR_7g077340	61.818	110	18	2	1	86	3	112	3.07e-38	124
MsG0780039678.01.T02	MTR_7g077215	62.887	97	15	1	11	86	14	110	4.18e-35	115
MsG0780039678.01.T02	MTR_7g077210	51.724	116	23	5	1	86	4	116	6.89e-25	90.1
MsG0780039678.01.T02	MTR_7g077300	48.544	103	35	3	1	86	1	102	1.80e-23	86.3
MsG0780039678.01.T02	MTR_7g077260	45.133	113	35	3	1	86	1	113	2.05e-22	83.6
MsG0780039678.01.T02	MTR_7g077310	47.115	104	28	2	9	86	1	103	4.67e-22	82.4
MsG0780039678.01.T02	MTR_7g077160	54.930	71	32	0	16	86	30	100	8.40e-21	79.3
MsG0780039678.01.T02	MTR_7g077220	46.875	96	16	2	8	80	1	84	1.45e-17	72.0
MsG0780039678.01.T02	MTR_7g077190	56.897	58	25	0	29	86	22	79	2.94e-17	69.7
MsG0780039678.01.T02	MTR_7g077313	38.384	99	39	3	3	81	4	100	7.80e-13	58.9
MsG0780039678.01.T02	MTR_7g077350	41.228	114	36	5	1	85	20	131	6.35e-11	54.7
MsG0780039678.01.T04	MTR_7g077180	96.825	63	2	0	1	63	24	86	1.37e-38	122
MsG0780039678.01.T04	MTR_7g077340	75.806	62	15	0	2	63	51	112	2.21e-28	98.2
MsG0780039678.01.T04	MTR_7g077215	75.000	60	15	0	4	63	51	110	4.94e-28	96.7
MsG0780039678.01.T04	MTR_7g077160	61.290	62	24	0	2	63	39	100	6.31e-20	76.3
MsG0780039678.01.T04	MTR_7g077310	64.407	59	21	0	5	63	45	103	6.36e-19	73.6
MsG0780039678.01.T04	MTR_7g077300	58.730	63	25	1	2	63	40	102	4.17e-18	71.6
MsG0780039678.01.T04	MTR_7g077190	56.897	58	25	0	6	63	22	79	3.93e-17	68.6
MsG0780039678.01.T04	MTR_7g077260	53.125	64	28	1	2	63	50	113	2.25e-16	67.4
MsG0780039678.01.T04	MTR_7g077210	58.730	63	20	3	4	63	57	116	7.43e-15	63.5
MsG0780039678.01.T03	MTR_7g077180	96.203	79	3	0	1	79	8	86	7.17e-50	152
MsG0780039678.01.T03	MTR_7g077340	62.745	102	15	1	1	79	11	112	1.67e-36	119
MsG0780039678.01.T03	MTR_7g077215	62.887	97	15	1	4	79	14	110	6.63e-35	114
MsG0780039678.01.T03	MTR_7g077210	50.926	108	21	4	1	79	12	116	3.95e-23	85.1
MsG0780039678.01.T03	MTR_7g077310	47.115	104	28	2	2	79	1	103	5.94e-22	82.0
MsG0780039678.01.T03	MTR_7g077300	46.875	96	34	2	1	79	7	102	2.23e-21	80.9
MsG0780039678.01.T03	MTR_7g077160	54.930	71	32	0	9	79	30	100	8.99e-21	79.0
MsG0780039678.01.T03	MTR_7g077260	43.269	104	34	2	1	79	10	113	3.23e-20	77.8
MsG0780039678.01.T03	MTR_7g077220	46.875	96	16	2	1	73	1	84	2.01e-17	71.2
MsG0780039678.01.T03	MTR_7g077190	56.897	58	25	0	22	79	22	79	3.39e-17	69.3
MsG0780039678.01.T03	MTR_7g077323	42.254	71	37	2	6	74	1	69	7.34e-11	52.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACATAGTTAATTACATTT+TGG	0.190001	7:-67452748	None:intergenic
AATGATGAAGTTAGCTTTGT+TGG	0.284953	7:+67452522	MsG0780039678.01.T02:CDS
TAGGCTTTGTTTATTGTGTA+TGG	0.302073	7:+67452805	MsG0780039678.01.T01:CDS
CAATGCCGTTGTCGTGATAT+TGG	0.339584	7:+67452634	MsG0780039678.01.T01:CDS
CTTGGGCTTCACTTCAACCA+TGG	0.414441	7:+67452552	MsG0780039678.01.T02:CDS
CAAAATGTAATTAACTATGT+TGG	0.531698	7:+67452749	MsG0780039678.01.T01:CDS
GTGATGAAACAAAGTCACTT+AGG	0.564264	7:+67452786	MsG0780039678.01.T01:CDS
ACTTTGTTTCATCACATGCA+TGG	0.577356	7:-67452778	None:intergenic
CACGACAACGGCATTGAGGA+GGG	0.589377	7:-67452627	None:intergenic
TCACGACAACGGCATTGAGG+AGG	0.620003	7:-67452628	None:intergenic
GCAGGAATCACAGCATGCAG+TGG	0.649347	7:-67452587	None:intergenic
TGGTTGAAGTGAAGCCCAAG+AGG	0.657022	7:-67452549	None:intergenic
ATATCACGACAACGGCATTG+AGG	0.657096	7:-67452631	None:intergenic
TCAGCGCAGTGACACTGTGG+AGG	0.657650	7:-67452706	None:intergenic
TTTCTCCAATATCACGACAA+CGG	0.709966	7:-67452639	None:intergenic
ATATCAGCGCAGTGACACTG+TGG	0.729166	7:-67452709	None:intergenic
AGTGGTTGTAGCATCAACCA+TGG	0.768073	7:-67452569	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAAATGTAATTAACTATGT+TGG	+	Chr7:67452749-67452768	MsG0780039678.01.T01:CDS	20.0%
!!!	CAACATAGTTAATTACATTT+TGG	-	Chr7:67452751-67452770	None:intergenic	20.0%
!!	AATGATGAAGTTAGCTTTGT+TGG	+	Chr7:67452522-67452541	MsG0780039678.01.T02:CDS	30.0%
!!	TAGGCTTTGTTTATTGTGTA+TGG	+	Chr7:67452805-67452824	MsG0780039678.01.T01:CDS	30.0%
	GTGATGAAACAAAGTCACTT+AGG	+	Chr7:67452786-67452805	MsG0780039678.01.T01:CDS	35.0%
	TTTCTCCAATATCACGACAA+CGG	-	Chr7:67452642-67452661	None:intergenic	35.0%
!	ACTTTGTTTCATCACATGCA+TGG	-	Chr7:67452781-67452800	None:intergenic	35.0%
!	GAGGAATAGATTTTGTGCAA+AGG	-	Chr7:67452690-67452709	None:intergenic	35.0%
!!	GAGGGATTGATTTTGTGCAA+GGG	-	Chr7:67452612-67452631	None:intergenic	40.0%
!!!	AGCTTTGTTGGTTTTCCTCT+TGG	+	Chr7:67452534-67452553	MsG0780039678.01.T02:CDS	40.0%
!!!	GCTTTGTTGGTTTTCCTCTT+GGG	+	Chr7:67452535-67452554	MsG0780039678.01.T02:CDS	40.0%
	AGTGGTTGTAGCATCAACCA+TGG	-	Chr7:67452572-67452591	None:intergenic	45.0%
	ATATCACGACAACGGCATTG+AGG	-	Chr7:67452634-67452653	None:intergenic	45.0%
	CAATGCCGTTGTCGTGATAT+TGG	+	Chr7:67452634-67452653	MsG0780039678.01.T01:CDS	45.0%
!!	GATTGATTTTGTGCAAGGGC+AGG	-	Chr7:67452608-67452627	None:intergenic	45.0%
!!	GGAGGGATTGATTTTGTGCA+AGG	-	Chr7:67452613-67452632	None:intergenic	45.0%
	ATATCAGCGCAGTGACACTG+TGG	-	Chr7:67452712-67452731	None:intergenic	50.0%
	CTTGGGCTTCACTTCAACCA+TGG	+	Chr7:67452552-67452571	MsG0780039678.01.T02:CDS	50.0%
	TGGTTGAAGTGAAGCCCAAG+AGG	-	Chr7:67452552-67452571	None:intergenic	50.0%
	GCAGGAATCACAGCATGCAG+TGG	-	Chr7:67452590-67452609	None:intergenic	55.0%
!	CACGACAACGGCATTGAGGA+GGG	-	Chr7:67452630-67452649	None:intergenic	55.0%
!	TCACGACAACGGCATTGAGG+AGG	-	Chr7:67452631-67452650	None:intergenic	55.0%
	TCAGCGCAGTGACACTGTGG+AGG	-	Chr7:67452709-67452728	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	67452502	67452841	67452502	ID=MsG0780039678.01;Name=MsG0780039678.01
Chr7	mRNA	67452578	67452841	67452578	ID=MsG0780039678.01.T01;Parent=MsG0780039678.01;Name=MsG0780039678.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|87
Chr7	exon	67452578	67452841	67452578	ID=MsG0780039678.01.T01:exon:11411;Parent=MsG0780039678.01.T01
Chr7	CDS	67452578	67452841	67452578	ID=MsG0780039678.01.T01:cds;Parent=MsG0780039678.01.T01
Chr7	mRNA	67452502	67452762	67452502	ID=MsG0780039678.01.T02;Parent=MsG0780039678.01;Name=MsG0780039678.01.T02;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|86
Chr7	exon	67452502	67452762	67452502	ID=MsG0780039678.01.T02:exon:11412;Parent=MsG0780039678.01.T02
Chr7	CDS	67452502	67452762	67452502	ID=MsG0780039678.01.T02:cds;Parent=MsG0780039678.01.T02
Chr7	mRNA	67452523	67452762	67452523	ID=MsG0780039678.01.T03;Parent=MsG0780039678.01;Name=MsG0780039678.01.T03;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|79
Chr7	exon	67452523	67452762	67452523	ID=MsG0780039678.01.T03:exon:11413;Parent=MsG0780039678.01.T03
Chr7	CDS	67452523	67452762	67452523	ID=MsG0780039678.01.T03:cds;Parent=MsG0780039678.01.T03
Chr7	mRNA	67452571	67452762	67452571	ID=MsG0780039678.01.T04;Parent=MsG0780039678.01;Name=MsG0780039678.01.T04;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|63
Chr7	exon	67452571	67452762	67452571	ID=MsG0780039678.01.T04:exon:11414;Parent=MsG0780039678.01.T04
Chr7	CDS	67452571	67452762	67452571	ID=MsG0780039678.01.T04:cds;Parent=MsG0780039678.01.T04
Chr7	mRNA	67452663	67452728	67452663	ID=MsG0780039678.01.T05;Parent=MsG0780039678.01;Name=MsG0780039678.01.T05;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|21
Chr7	exon	67452663	67452728	67452663	ID=MsG0780039678.01.T05:exon:11415;Parent=MsG0780039678.01.T05
Chr7	CDS	67452663	67452728	67452663	ID=MsG0780039678.01.T05:cds;Parent=MsG0780039678.01.T05

Gene Sequence

>MsG0780039678.01.T02

ATGGAGTTGAACAAGAAGACAATGATGAAGTTAGCTTTGTTGGTTTTCCTCTTGGGCTTCACTTCAACCATGGTTGATGCTACAACCACTGCATGCTGTGATTCCTGCCCTTGCACAAAATCAATCCCTCCTCAATGCCGTTGTCGTGATATTGGAGAAACATGTCACTCAGCTTGCAAAAGCTGCCTTTGCACAAAATCTATTCCTCCACAGTGTCACTGCGCTGATATCACTGACTTCTGCTATCCAAAATGTAATTAA

>MsG0780039678.01.T01

ATGCTACAACCACTGCATGCTGTGATTCCTGCCCTTGCACAAAATCAATCCCTCCTCAATGCCGTTGTCGTGATATTGGAGAAACATGTCACTCAGCTTGCAAAAGCTGCCTTTGCACAAAATCTATTCCTCCACAGTGTCACTGCGCTGATATCACTGACTTCTGCTATCCAAAATGTAATTAACTATGTTGGTATATACCATGCATGTGATGAAACAAAGTCACTTAGGCTTTGTTTATTGTGTATGGAAATGAATAAATAA

>MsG0780039678.01.T04

ATGGTTGATGCTACAACCACTGCATGCTGTGATTCCTGCCCTTGCACAAAATCAATCCCTCCTCAATGCCGTTGTCGTGATATTGGAGAAACATGTCACTCAGCTTGCAAAAGCTGCCTTTGCACAAAATCTATTCCTCCACAGTGTCACTGCGCTGATATCACTGACTTCTGCTATCCAAAATGTAATTAA

>MsG0780039678.01.T03

ATGATGAAGTTAGCTTTGTTGGTTTTCCTCTTGGGCTTCACTTCAACCATGGTTGATGCTACAACCACTGCATGCTGTGATTCCTGCCCTTGCACAAAATCAATCCCTCCTCAATGCCGTTGTCGTGATATTGGAGAAACATGTCACTCAGCTTGCAAAAGCTGCCTTTGCACAAAATCTATTCCTCCACAGTGTCACTGCGCTGATATCACTGACTTCTGCTATCCAAAATGTAATTAA

>MsG0780039678.01.T05

ATGTCACTCAGCTTGCAAAAGCTGCCTTTGCACAAAATCTATTCCTCCACAGTGTCACTGCGCTGA

Protein sequence

>MsG0780039678.01.T02

MELNKKTMMKLALLVFLLGFTSTMVDATTTACCDSCPCTKSIPPQCRCRDIGETCHSACKSCLCTKSIPPQCHCADITDFCYPKCN*

>MsG0780039678.01.T01

MLQPLHAVIPALAQNQSLLNAVVVILEKHVTQLAKAAFAQNLFLHSVTALISLTSAIQNVINYVGIYHACDETKSLRLCLLCMEMNK*

>MsG0780039678.01.T04

MVDATTTACCDSCPCTKSIPPQCRCRDIGETCHSACKSCLCTKSIPPQCHCADITDFCYPKCN*

>MsG0780039678.01.T03

MMKLALLVFLLGFTSTMVDATTTACCDSCPCTKSIPPQCRCRDIGETCHSACKSCLCTKSIPPQCHCADITDFCYPKCN*

>MsG0780039678.01.T05

MSLSLQKLPLHKIYSSTVSLR*