AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039890.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039890.01.T01 MTR_7g081070 99.213 127 1 0 1 127 1 127 5.84e-89 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039890.01.T01 AT2G20940 60.800 125 46 1 5 126 4 128 8.94e-52 160
MsG0780039890.01.T01 AT2G20940 60.800 125 46 1 5 126 4 128 8.94e-52 160

Find 34 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGCTCTGATTCCTGTTC+GGG 0.317971 7:-70161042 MsG0780039890.01.T01:CDS
GCGCAGATGGCGGCTTCTGC+TGG 0.326079 7:-70161095 MsG0780039890.01.T01:CDS
AAGAATCAGTATCAGAATTA+AGG 0.347381 7:+70161144 None:intergenic
ACAAGGCTCTGATTCCTGTT+CGG 0.366143 7:-70161043 MsG0780039890.01.T01:CDS
TCAGTATCAGAATTAAGGTT+AGG 0.368311 7:+70161149 None:intergenic
AACGTCGACGTTGTTTCTAA+TGG 0.376811 7:+70161223 None:intergenic
GGCGACGGAAGCGGCGGAAA+CGG 0.412713 7:+70161259 None:intergenic
ATAGTGATGGGAACCCGAAC+AGG 0.445276 7:+70161029 None:intergenic
GTTTCCGCCGCTTCCGTCGC+CGG 0.450661 7:-70161257 MsG0780039890.01.T01:CDS
CAGTATCAGAATTAAGGTTA+GGG 0.462709 7:+70161150 None:intergenic
TGCGCCGTGCGATTCTTCAC+TGG 0.482560 7:+70161113 None:intergenic
CAGATGGCGGCTTCTGCTGG+TGG 0.491218 7:-70161092 MsG0780039890.01.T01:CDS
CCTAGCTGTGCTTTGCAACA+AGG 0.506591 7:-70161060 MsG0780039890.01.T01:CDS
CCTTGTTGCAAAGCACAGCT+AGG 0.511786 7:+70161060 None:intergenic
CGCACCTCACAAACTATGTC+CGG 0.513022 7:-70161341 None:intergenic
GTAGATGCCGGCGACGGAAG+CGG 0.518756 7:+70161250 None:intergenic
GAAGAATCGCACGGCGCAGA+TGG 0.519084 7:-70161108 MsG0780039890.01.T01:CDS
AATCCTCGAGAAATTTCACT+TGG 0.549671 7:-70161195 MsG0780039890.01.T01:CDS
TCTAATGGCGACGTAGATGC+CGG 0.552367 7:+70161238 None:intergenic
AGGAGGAAGATTGTGAAGAC+TGG 0.552576 7:-70160972 MsG0780039890.01.T01:CDS
AAGTACGGGAAAGTAGCAAT+CGG 0.559634 7:-70161293 MsG0780039890.01.T01:CDS
GAGCTCGCGGAATCGACCAC+CGG 0.566038 7:+70161322 None:intergenic
GGGCGTGAGTGCTATAGTGA+TGG 0.566302 7:+70161016 None:intergenic
GAATCGCACGGCGCAGATGG+CGG 0.577526 7:-70161105 MsG0780039890.01.T01:CDS
CTTGTTGCAAAGCACAGCTA+GGG 0.588257 7:+70161061 None:intergenic
CGTGCGATTCTTCACTGGCA+CGG 0.590678 7:+70161118 None:intergenic
GGCGACGTAGATGCCGGCGA+CGG 0.595129 7:+70161244 None:intergenic
GATGCCGGCGACGGAAGCGG+CGG 0.604989 7:+70161253 None:intergenic
CACCCAAGTGAAATTTCTCG+AGG 0.622021 7:+70161192 None:intergenic
GGCGTGAGTGCTATAGTGAT+GGG 0.635390 7:+70161017 None:intergenic
ATCCTCGAGAAATTTCACTT+GGG 0.664906 7:-70161194 MsG0780039890.01.T01:CDS
ACCTCACAAACTATGTCCGG+TGG 0.670449 7:-70161338 None:intergenic
CGTGCCAGTGAAGAATCGCA+CGG 0.698432 7:-70161117 MsG0780039890.01.T01:CDS
GCGATTCTTCACTGGCACGG+TGG 0.698621 7:+70161121 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAATCAGTATCAGAATTA+AGG + Chr7:70161150-70161169 None:intergenic 25.0%
CAGTATCAGAATTAAGGTTA+GGG + Chr7:70161144-70161163 None:intergenic 30.0%
TCAGTATCAGAATTAAGGTT+AGG + Chr7:70161145-70161164 None:intergenic 30.0%
! ATTAAGGTTAGGGTTTTCTT+CGG + Chr7:70161134-70161153 None:intergenic 30.0%
AATCCTCGAGAAATTTCACT+TGG - Chr7:70161096-70161115 MsG0780039890.01.T01:CDS 35.0%
ATCCTCGAGAAATTTCACTT+GGG - Chr7:70161097-70161116 MsG0780039890.01.T01:CDS 35.0%
! TTAAGGTTAGGGTTTTCTTC+GGG + Chr7:70161133-70161152 None:intergenic 35.0%
AAGTACGGGAAAGTAGCAAT+CGG - Chr7:70160998-70161017 MsG0780039890.01.T01:CDS 40.0%
TTGCCAAAAACCTCGCAATT+GGG + Chr7:70161300-70161319 None:intergenic 40.0%
!! AACGTCGACGTTGTTTCTAA+TGG + Chr7:70161071-70161090 None:intergenic 40.0%
!!! CTACTTTCCCGTACTTTTTC+AGG + Chr7:70160994-70161013 None:intergenic 40.0%
AGGAGGAAGATTGTGAAGAC+TGG - Chr7:70161319-70161338 MsG0780039890.01.T01:CDS 45.0%
CACCCAAGTGAAATTTCTCG+AGG + Chr7:70161102-70161121 None:intergenic 45.0%
CTTGCCAAAAACCTCGCAAT+TGG + Chr7:70161301-70161320 None:intergenic 45.0%
CTTGTTGCAAAGCACAGCTA+GGG + Chr7:70161233-70161252 None:intergenic 45.0%
TGCCAAAAACCTCGCAATTG+GGG + Chr7:70161299-70161318 None:intergenic 45.0%
! ACAAGGCTCTGATTCCTGTT+CGG - Chr7:70161248-70161267 MsG0780039890.01.T01:CDS 45.0%
!!! CAATTGCGAGGTTTTTGGCA+AGG - Chr7:70161299-70161318 MsG0780039890.01.T01:CDS 45.0%
CCTAGCTGTGCTTTGCAACA+AGG - Chr7:70161231-70161250 MsG0780039890.01.T01:CDS 50.0%
CCTTGTTGCAAAGCACAGCT+AGG + Chr7:70161234-70161253 None:intergenic 50.0%
CGCGAGCTCCTGAAAAAGTA+CGG - Chr7:70160983-70161002 MsG0780039890.01.T01:CDS 50.0%
GCCAAAAACCTCGCAATTGG+GGG + Chr7:70161298-70161317 None:intergenic 50.0%
GGCGTGAGTGCTATAGTGAT+GGG + Chr7:70161277-70161296 None:intergenic 50.0%
TCTAATGGCGACGTAGATGC+CGG + Chr7:70161056-70161075 None:intergenic 50.0%
! AGGGTTTTCTTCGGGAGAAG+CGG + Chr7:70161125-70161144 None:intergenic 50.0%
! ATAGTGATGGGAACCCGAAC+AGG + Chr7:70161265-70161284 None:intergenic 50.0%
! CAAGGCTCTGATTCCTGTTC+GGG - Chr7:70161249-70161268 MsG0780039890.01.T01:CDS 50.0%
! GCGAGCTCCTGAAAAAGTAC+GGG - Chr7:70160984-70161003 MsG0780039890.01.T01:CDS 50.0%
!!! CGTACTTTTTCAGGAGCTCG+CGG + Chr7:70160985-70161004 None:intergenic 50.0%
!!! TTGCGAGGTTTTTGGCAAGG+AGG - Chr7:70161302-70161321 MsG0780039890.01.T01:CDS 50.0%
CGTGCCAGTGAAGAATCGCA+CGG - Chr7:70161174-70161193 MsG0780039890.01.T01:CDS 55.0%
CGTGCGATTCTTCACTGGCA+CGG + Chr7:70161176-70161195 None:intergenic 55.0%
GGGCGTGAGTGCTATAGTGA+TGG + Chr7:70161278-70161297 None:intergenic 55.0%
! GCCCCCAATTGCGAGGTTTT+TGG - Chr7:70161294-70161313 MsG0780039890.01.T01:CDS 55.0%
GAAGAATCGCACGGCGCAGA+TGG - Chr7:70161183-70161202 MsG0780039890.01.T01:CDS 60.0%
GCGATTCTTCACTGGCACGG+TGG + Chr7:70161173-70161192 None:intergenic 60.0%
TGCGCCGTGCGATTCTTCAC+TGG + Chr7:70161181-70161200 None:intergenic 60.0%
CACTCACGCCCCCAATTGCG+AGG - Chr7:70161287-70161306 MsG0780039890.01.T01:CDS 65.0%
GAATCGCACGGCGCAGATGG+CGG - Chr7:70161186-70161205 MsG0780039890.01.T01:CDS 65.0%
GAGCTCGCGGAATCGACCAC+CGG + Chr7:70160972-70160991 None:intergenic 65.0%
GTAGATGCCGGCGACGGAAG+CGG + Chr7:70161044-70161063 None:intergenic 65.0%
! CAGATGGCGGCTTCTGCTGG+TGG - Chr7:70161199-70161218 MsG0780039890.01.T01:CDS 65.0%
GGCGACGGAAGCGGCGGAAA+CGG + Chr7:70161035-70161054 None:intergenic 70.0%
GGCGACGTAGATGCCGGCGA+CGG + Chr7:70161050-70161069 None:intergenic 70.0%
GTTTCCGCCGCTTCCGTCGC+CGG - Chr7:70161034-70161053 MsG0780039890.01.T01:CDS 70.0%
! GCGCAGATGGCGGCTTCTGC+TGG - Chr7:70161196-70161215 MsG0780039890.01.T01:CDS 70.0%
GATGCCGGCGACGGAAGCGG+CGG + Chr7:70161041-70161060 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr7 gene 70160965 70161348 70160965 ID=MsG0780039890.01;Name=MsG0780039890.01
Chr7 mRNA 70160965 70161348 70160965 ID=MsG0780039890.01.T01;Parent=MsG0780039890.01;Name=MsG0780039890.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|127
Chr7 exon 70160965 70161348 70160965 ID=MsG0780039890.01.T01:exon:13045;Parent=MsG0780039890.01.T01
Chr7 CDS 70160965 70161348 70160965 ID=MsG0780039890.01.T01:cds;Parent=MsG0780039890.01.T01
Gene Sequence

>MsG0780039890.01.T01

ATGTCCGGTGGTCGATTCCGCGAGCTCCTGAAAAAGTACGGGAAAGTAGCAATCGGCGTTCACTGCTCCGTTTCCGCCGCTTCCGTCGCCGGCATCTACGTCGCCATTAGAAACAACGTCGACGTTGAAGCAATCCTCGAGAAATTTCACTTGGGTGCCGCTTCTCCCGAAGAAAACCCTAACCTTAATTCTGATACTGATTCTTCCACCGTGCCAGTGAAGAATCGCACGGCGCAGATGGCGGCTTCTGCTGGTGGTGCTTTTACCCTAGCTGTGCTTTGCAACAAGGCTCTGATTCCTGTTCGGGTTCCCATCACTATAGCACTCACGCCCCCAATTGCGAGGTTTTTGGCAAGGAGGAAGATTGTGAAGACTGGTGTATGA
Protein sequence

>MsG0780039890.01.T01

MSGGRFRELLKKYGKVAIGVHCSVSAASVAGIYVAIRNNVDVEAILEKFHLGAASPEENPNLNSDTDSSTVPVKNRTAQMAASAGGAFTLAVLCNKALIPVRVPITIALTPPIARFLARRKIVKTGV*