AlfalfaGEDB | MsG0780039906.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039906.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039906.01.T01	XP_024628663.1	71.111	180	23	1	1	151	2	181	6.79E-82	249
MsG0780039906.01.T02	XP_024628663.1	85.841	113	16	0	21	133	69	181	5.19E-62	198

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039906.01.T01	A0A2Z6NH40	45.143	175	67	2	5	150	215	389	2.95e-37	141
MsG0780039906.01.T02	A0A392N2E8	52.252	111	53	0	22	132	7	117	1.77e-34	125

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0780039906.01	MsG0780041116.01	PPI
MsG0280009322.01	MsG0780039906.01	PPI
MsG0780039906.01	MsG0280010449.01	PPI
MsG0780040274.01	MsG0780039906.01	PPI
MsG0780039906.01	MsG0780041116.01	PPI
MsG0780039906.01	MsG0380013578.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039906.01.T01	MTR_3g010950	43.119	109	57	2	38	144	5	110	3.60e-22	86.3
MsG0780039906.01.T01	MTR_8g028785	41.739	115	62	1	28	137	251	365	1.86e-21	89.0
MsG0780039906.01.T01	MTR_7g006380	43.750	112	61	1	35	144	42	153	3.73e-21	84.7
MsG0780039906.01.T01	MTR_1g103580	39.231	130	51	3	24	148	120	226	1.04e-19	82.8
MsG0780039906.01.T01	MTR_8g022750	43.137	102	46	1	38	127	184	285	9.42e-19	80.5
MsG0780039906.01.T01	MTR_8g031080	36.508	126	73	3	28	148	258	381	9.14e-18	79.0
MsG0780039906.01.T01	MTR_2g047100	41.000	100	45	2	44	129	3	102	2.71e-17	72.8
MsG0780039906.01.T01	MTR_5g083510	58.929	56	23	0	93	148	17	72	3.22e-15	67.0
MsG0780039906.01.T01	MTR_6g045427	40.000	85	50	1	37	120	151	235	1.61e-14	68.6
MsG0780039906.01.T01	MTR_5g031620	54.545	66	30	0	85	150	139	204	2.11e-14	68.2
MsG0780039906.01.T01	MTR_2g027020	33.333	132	63	2	38	144	7	138	7.39e-14	65.9
MsG0780039906.01.T01	MTR_1g057740	32.877	146	73	2	5	150	3	123	7.81e-14	64.7
MsG0780039906.01.T01	MTR_5g037920	32.374	139	65	2	39	148	78	216	1.07e-13	66.2
MsG0780039906.01.T01	MTR_5g036320	39.583	96	29	1	28	94	48	143	1.41e-13	64.3
MsG0780039906.01.T01	MTR_3g116210	34.513	113	45	1	38	121	76	188	2.08e-13	64.7
MsG0780039906.01.T01	MTR_2g102247	46.875	64	34	0	5	68	75	138	6.19e-13	62.8
MsG0780039906.01.T01	MTR_7g063280	36.792	106	38	2	56	132	69	174	1.90e-12	62.0
MsG0780039906.01.T01	MTR_7g033395	40.299	67	40	0	1	67	58	124	4.39e-12	60.1
MsG0780039906.01.T01	MTR_5g022720	42.857	70	40	0	79	148	3	72	1.10e-11	61.2
MsG0780039906.01.T01	MTR_4g029360	27.711	166	81	5	4	130	62	227	2.95e-11	59.7
MsG0780039906.01.T01	MTR_4g035890	39.706	68	41	0	1	68	56	123	4.42e-11	57.8
MsG0780039906.01.T01	MTR_1g101650	29.518	166	94	5	1	149	22	181	8.22e-11	57.8
MsG0780039906.01.T02	MTR_7g063280	42.553	94	54	0	21	114	81	174	3.60e-23	89.4
MsG0780039906.01.T02	MTR_5g075610	50.000	96	46	1	21	116	4	97	2.01e-20	80.1
MsG0780039906.01.T02	MTR_6g034915	42.727	110	46	1	21	130	64	156	6.38e-19	78.2
MsG0780039906.01.T02	MTR_2g027020	42.453	106	57	1	21	126	37	138	6.56e-19	78.6
MsG0780039906.01.T02	MTR_5g083200	42.045	88	51	0	21	108	107	194	1.98e-17	74.7
MsG0780039906.01.T02	MTR_0002s1220	42.045	88	51	0	21	108	107	194	1.98e-17	74.7
MsG0780039906.01.T02	MTR_5g069700	51.429	70	34	0	34	103	3	72	2.96e-17	71.2
MsG0780039906.01.T02	MTR_3g111390	42.045	88	51	0	21	108	107	194	4.56e-17	73.9
MsG0780039906.01.T02	MTR_1g070820	42.045	88	51	0	21	108	107	194	4.56e-17	73.9
MsG0780039906.01.T02	MTR_3g116210	40.964	83	49	0	21	103	106	188	5.86e-17	73.6
MsG0780039906.01.T02	MTR_1g045340	40.909	88	52	0	21	108	93	180	6.53e-17	73.2
MsG0780039906.01.T02	MTR_5g037920	41.818	110	62	2	22	130	108	216	7.66e-17	73.9
MsG0780039906.01.T02	MTR_8g088220	44.156	77	43	0	19	95	1	77	1.95e-16	69.3
MsG0780039906.01.T02	MTR_6g016265	44.872	78	43	0	21	98	48	125	4.51e-16	69.7
MsG0780039906.01.T02	MTR_8g098925	37.500	104	62	1	9	109	59	162	9.16e-16	69.7
MsG0780039906.01.T02	MTR_8g028785	47.475	99	48	2	25	119	267	365	1.01e-15	72.4
MsG0780039906.01.T02	MTR_5g083510	58.929	56	23	0	75	130	17	72	2.07e-15	67.0
MsG0780039906.01.T02	MTR_5g036320	58.929	56	23	0	21	76	88	143	4.40e-15	67.8
MsG0780039906.01.T02	MTR_1g069475	43.662	71	40	0	21	91	173	243	4.72e-15	69.7
MsG0780039906.01.T02	MTR_5g031620	54.545	66	30	0	67	132	139	204	1.28e-14	67.8
MsG0780039906.01.T02	MTR_8g059135	41.176	85	50	0	22	106	38	122	2.32e-14	65.1
MsG0780039906.01.T02	MTR_4g029360	41.304	92	54	0	21	112	136	227	2.79e-14	67.0
MsG0780039906.01.T02	MTR_0015s0120	50.769	65	32	0	21	85	302	366	7.51e-14	67.0
MsG0780039906.01.T02	MTR_1g045560	38.144	97	44	2	30	126	13	93	9.83e-14	62.8
MsG0780039906.01.T02	MTR_1g088650	43.158	95	54	0	21	115	95	189	1.76e-13	64.3
MsG0780039906.01.T02	MTR_1g050488	52.632	57	27	0	22	78	108	164	3.02e-13	63.2
MsG0780039906.01.T02	MTR_1g081930	43.038	79	45	0	21	99	47	125	3.20e-13	62.4
MsG0780039906.01.T02	MTR_4g064590	40.217	92	49	2	36	125	50	137	7.46e-13	62.0
MsG0780039906.01.T02	MTR_0093s0070	39.189	74	45	0	21	94	132	205	1.07e-12	62.4
MsG0780039906.01.T02	MTR_6g453270	42.857	70	40	0	21	90	309	378	2.28e-12	63.2
MsG0780039906.01.T02	MTR_2g059470	40.541	74	44	0	22	95	10	83	6.10e-12	57.8
MsG0780039906.01.T02	MTR_5g022720	42.857	70	40	0	61	130	3	72	6.74e-12	61.2
MsG0780039906.01.T02	MTR_1g062840	40.278	72	43	0	36	107	4	75	1.02e-11	57.4
MsG0780039906.01.T02	MTR_2g088140	46.552	58	31	0	73	130	49	106	3.98e-11	56.6
MsG0780039906.01.T02	MTR_3g090920	35.354	99	47	3	30	128	5	86	4.18e-11	56.2
MsG0780039906.01.T02	MTR_4g008270	40.000	75	45	0	21	95	19	93	6.06e-11	55.5
MsG0780039906.01.T02	MTR_8g022750	44.444	72	40	0	38	109	214	285	9.25e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCAAGGGTAGAGTTTATT+AGG	0.257478	7:+70295424	MsG0780039906.01.T01:CDS
ATACATCATTTCTAGTGTTA+TGG	0.321538	7:-70295344	None:intergenic
CGGCGTTGACCAACTAGTTC+TGG	0.340697	7:-70295202	None:intergenic
GAAAGCAACCAGGTTGGTAT+AGG	0.348938	7:+70295142	MsG0780039906.01.T01:CDS
CTATCTCATTTCATTAATAG+TGG	0.357792	7:-70295506	None:intergenic
GTTGTACACCCGCTCTATTA+CGG	0.414511	7:-70295039	None:intergenic
ATCGTATTGATGACACAGTT+TGG	0.416348	7:+70295065	MsG0780039906.01.T01:CDS
AAAGCAACCAGGTTGGTATA+GGG	0.456165	7:+70295143	MsG0780039906.01.T01:CDS
TTGGTATAGGGATATGCATC+AGG	0.476453	7:+70295155	MsG0780039906.01.T01:CDS
TGCTATCAGATGGGCAAAGA+AGG	0.492765	7:+70295266	MsG0780039906.01.T01:intron
CATTTGAAATGACCTACATC+CGG	0.493186	7:-70295094	None:intergenic
GAGCTATTATCAGAGAATGT+AGG	0.524679	7:+70295376	MsG0780039906.01.T01:CDS
AGTGCAGCAGTTTGTGATAG+AGG	0.536324	7:+70294977	MsG0780039906.01.T01:CDS
TATTAGGAGACAAGCAAATG+AGG	0.544073	7:+70295440	MsG0780039906.01.T01:CDS
GTTTGGAGAAAGCCGGATGT+AGG	0.547419	7:+70295082	MsG0780039906.01.T01:CDS
AGGTTGCTCATAGACTTGCT+AGG	0.556138	7:+70295460	MsG0780039906.01.T01:CDS
CAAGAAAGCCGTAATAGAGC+GGG	0.562609	7:+70295031	MsG0780039906.01.T01:CDS
TGACACAGTTTGGAGAAAGC+CGG	0.567834	7:+70295075	MsG0780039906.01.T01:CDS
TGCTCATAGACTTGCTAGGG+TGG	0.576889	7:+70295464	MsG0780039906.01.T01:CDS
GGTTGCTCATAGACTTGCTA+GGG	0.594727	7:+70295461	MsG0780039906.01.T01:CDS
TTTCGAGCTCGATTCTAAGA+GGG	0.602890	7:+70295308	MsG0780039906.01.T01:CDS
TTCAAAGAAAGCAACCAGGT+TGG	0.607814	7:+70295136	MsG0780039906.01.T01:CDS
TAGTGAAAGCCAGAACTAGT+TGG	0.614923	7:+70295193	MsG0780039906.01.T01:intron
TGCATATCCCTATACCAACC+TGG	0.616873	7:-70295150	None:intergenic
GCAAGAAAGCCGTAATAGAG+CGG	0.632648	7:+70295030	MsG0780039906.01.T01:CDS
TGCATCAGGATGATCACGGA+AGG	0.633337	7:+70295169	MsG0780039906.01.T01:intron
GTGGATACTAGCATAAAATG+TGG	0.637527	7:-70295487	None:intergenic
GATATGCATCAGGATGATCA+CGG	0.669365	7:+70295165	MsG0780039906.01.T01:CDS
GTTCTTCAAAGAAAGCAACC+AGG	0.680960	7:+70295132	MsG0780039906.01.T01:CDS
CCCTCGAAAACAGCTAAGAG+CGG	0.703336	7:-70295222	None:intergenic
AGGTCATTTCAAATGCAATG+TGG	0.742639	7:+70295102	MsG0780039906.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTTTATATGCTATCAGAT+GGG	+	Chr7:70295257-70295276	MsG0780039906.01.T01:intron	25.0%
!!!	ATACATCATTTCTAGTGTTA+TGG	-	Chr7:70295347-70295366	None:intergenic	25.0%
!	AGAGGTCTTAATCTTTTAAC+TGG	+	Chr7:70294995-70295014	MsG0780039906.01.T01:CDS	30.0%
!	GTCTTTTATATGCTATCAGA+TGG	+	Chr7:70295256-70295275	MsG0780039906.01.T01:intron	30.0%
!!	GTCTTAATCTTTTAACTGGA+TGG	+	Chr7:70294999-70295018	MsG0780039906.01.T01:CDS	30.0%
!!!	ATGCTTTTTCACAAACTCAA+GGG	+	Chr7:70295410-70295429	MsG0780039906.01.T01:CDS	30.0%
	ACTCAAGGGTAGAGTTTATT+AGG	+	Chr7:70295424-70295443	MsG0780039906.01.T01:CDS	35.0%
	AGGTCATTTCAAATGCAATG+TGG	+	Chr7:70295102-70295121	MsG0780039906.01.T01:CDS	35.0%
	GAGCTATTATCAGAGAATGT+AGG	+	Chr7:70295376-70295395	MsG0780039906.01.T01:CDS	35.0%
	GTGGATACTAGCATAAAATG+TGG	-	Chr7:70295490-70295509	None:intergenic	35.0%
	TATTAGGAGACAAGCAAATG+AGG	+	Chr7:70295440-70295459	MsG0780039906.01.T01:CDS	35.0%
!	ATCGTATTGATGACACAGTT+TGG	+	Chr7:70295065-70295084	MsG0780039906.01.T01:CDS	35.0%
!	CATTTGAAATGACCTACATC+CGG	-	Chr7:70295097-70295116	None:intergenic	35.0%
!!!	CATGCTTTTTCACAAACTCA+AGG	+	Chr7:70295409-70295428	MsG0780039906.01.T01:CDS	35.0%
	GATATGCATCAGGATGATCA+CGG	+	Chr7:70295165-70295184	MsG0780039906.01.T01:CDS	40.0%
	GTTCTTCAAAGAAAGCAACC+AGG	+	Chr7:70295132-70295151	MsG0780039906.01.T01:CDS	40.0%
	TAGTGAAAGCCAGAACTAGT+TGG	+	Chr7:70295193-70295212	MsG0780039906.01.T01:intron	40.0%
	TTCAAAGAAAGCAACCAGGT+TGG	+	Chr7:70295136-70295155	MsG0780039906.01.T01:CDS	40.0%
	TTGGTATAGGGATATGCATC+AGG	+	Chr7:70295155-70295174	MsG0780039906.01.T01:CDS	40.0%
!	TTTCGAGCTCGATTCTAAGA+GGG	+	Chr7:70295308-70295327	MsG0780039906.01.T01:CDS	40.0%
!!	AAAGCAACCAGGTTGGTATA+GGG	+	Chr7:70295143-70295162	MsG0780039906.01.T01:CDS	40.0%
!!	TTTTCGAGCTCGATTCTAAG+AGG	+	Chr7:70295307-70295326	MsG0780039906.01.T01:CDS	40.0%
	AGTGCAGCAGTTTGTGATAG+AGG	+	Chr7:70294977-70294996	MsG0780039906.01.T01:CDS	45.0%
	CAAGAAAGCCGTAATAGAGC+GGG	+	Chr7:70295031-70295050	MsG0780039906.01.T01:CDS	45.0%
	GCAAGAAAGCCGTAATAGAG+CGG	+	Chr7:70295030-70295049	MsG0780039906.01.T01:CDS	45.0%
	GTTGTACACCCGCTCTATTA+CGG	-	Chr7:70295042-70295061	None:intergenic	45.0%
	TGACACAGTTTGGAGAAAGC+CGG	+	Chr7:70295075-70295094	MsG0780039906.01.T01:CDS	45.0%
	TGCATATCCCTATACCAACC+TGG	-	Chr7:70295153-70295172	None:intergenic	45.0%
	TGCTATCAGATGGGCAAAGA+AGG	+	Chr7:70295266-70295285	MsG0780039906.01.T01:intron	45.0%
!	AGGTTGCTCATAGACTTGCT+AGG	+	Chr7:70295460-70295479	MsG0780039906.01.T01:CDS	45.0%
!!	GAAAGCAACCAGGTTGGTAT+AGG	+	Chr7:70295142-70295161	MsG0780039906.01.T01:CDS	45.0%
!!	GGTTGCTCATAGACTTGCTA+GGG	+	Chr7:70295461-70295480	MsG0780039906.01.T01:CDS	45.0%
	CCCTCGAAAACAGCTAAGAG+CGG	-	Chr7:70295225-70295244	None:intergenic	50.0%
	GTTTGGAGAAAGCCGGATGT+AGG	+	Chr7:70295082-70295101	MsG0780039906.01.T01:CDS	50.0%
	TGCATCAGGATGATCACGGA+AGG	+	Chr7:70295169-70295188	MsG0780039906.01.T01:intron	50.0%
!!	CCGCTCTTAGCTGTTTTCGA+GGG	+	Chr7:70295222-70295241	MsG0780039906.01.T01:intron	50.0%
!!	CTTAGCTGTTTTCGAGGGAG+AGG	+	Chr7:70295227-70295246	MsG0780039906.01.T01:intron	50.0%
!!	TGCTCATAGACTTGCTAGGG+TGG	+	Chr7:70295464-70295483	MsG0780039906.01.T01:CDS	50.0%
!!	CGGCGTTGACCAACTAGTTC+TGG	-	Chr7:70295205-70295224	None:intergenic	55.0%
!!	GCCGCTCTTAGCTGTTTTCG+AGG	+	Chr7:70295221-70295240	MsG0780039906.01.T01:intron	55.0%
!!	GTTTTCGAGGGAGAGGCTCT+TGG	+	Chr7:70295234-70295253	MsG0780039906.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	70294974	70295515	70294974	ID=MsG0780039906.01;Name=MsG0780039906.01
Chr7	mRNA	70294974	70295515	70294974	ID=MsG0780039906.01.T01;Parent=MsG0780039906.01;Name=MsG0780039906.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|151
Chr7	exon	70294974	70295185	70294974	ID=MsG0780039906.01.T01:exon:13081;Parent=MsG0780039906.01.T01
Chr7	exon	70295272	70295515	70295272	ID=MsG0780039906.01.T01:exon:13082;Parent=MsG0780039906.01.T01
Chr7	CDS	70294974	70295185	70294974	ID=MsG0780039906.01.T01:cds;Parent=MsG0780039906.01.T01
Chr7	CDS	70295272	70295515	70295272	ID=MsG0780039906.01.T01:cds;Parent=MsG0780039906.01.T01
Chr7	mRNA	70295114	70295515	70295114	ID=MsG0780039906.01.T02;Parent=MsG0780039906.01;Name=MsG0780039906.01.T02;_AED=0.50;_eAED=0.56;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|133
Chr7	exon	70295114	70295515	70295114	ID=MsG0780039906.01.T02:exon:13083;Parent=MsG0780039906.01.T02
Chr7	CDS	70295114	70295515	70295114	ID=MsG0780039906.01.T02:cds;Parent=MsG0780039906.01.T02

Gene Sequence

>MsG0780039906.01.T01

ACTAGTGCAGCAGTTTGTGATAGAGGTCTTAATCTTTTAACTGGATGGCAAAACGTGCAAGAAAGCCGTAATAGAGCGGGTGTACAACAACATCGTATTGATGACACAGTTTGGAGAAAGCCGGATGTAGGTCATTTCAAATGCAATGTGGATGCTGCGTTCTTCAAAGAAAGCAACCAGGTTGGTATAGGGATATGCATCAGGATGATCACCAGATGGGCAAAGAAGGAGATTTTTAATAATGTCATTTTCGAGCTCGATTCTAAGAGGGTAGTAGATAGTTTCCATAACACTAGAAATGATGTATCTGATTTAAGAGCTATTATCAGAGAATGTAGGAATACATTCTCATGCTTTTTCACAAACTCAAGGGTAGAGTTTATTAGGAGACAAGCAAATGAGGTTGCTCATAGACTTGCTAGGGTGGCCACATTTTATGCTAGTATCCACTATTAA

>MsG0780039906.01.T02

ATGCAATGTGGATGCTGCGTTCTTCAAAGAAAGCAACCAGGTTGGTATAGGGATATGCATCAGGATGATCACGGAAGGTTAGTGAAAGCCAGAACTAGTTGGTCAACGCCGCTCTTAGCTGTTTTCGAGGGAGAGGCTCTTGGTCTTTTATATGCTATCAGATGGGCAAAGAAGGAGATTTTTAATAATGTCATTTTCGAGCTCGATTCTAAGAGGGTAGTAGATAGTTTCCATAACACTAGAAATGATGTATCTGATTTAAGAGCTATTATCAGAGAATGTAGGAATACATTCTCATGCTTTTTCACAAACTCAAGGGTAGAGTTTATTAGGAGACAAGCAAATGAGGTTGCTCATAGACTTGCTAGGGTGGCCACATTTTATGCTAGTATCCACTATTAA

Protein sequence

>MsG0780039906.01.T01

TSAAVCDRGLNLLTGWQNVQESRNRAGVQQHRIDDTVWRKPDVGHFKCNVDAAFFKESNQVGIGICIRMITRWAKKEIFNNVIFELDSKRVVDSFHNTRNDVSDLRAIIRECRNTFSCFFTNSRVEFIRRQANEVAHRLARVATFYASIHY*

>MsG0780039906.01.T02

MQCGCCVLQRKQPGWYRDMHQDDHGRLVKARTSWSTPLLAVFEGEALGLLYAIRWAKKEIFNNVIFELDSKRVVDSFHNTRNDVSDLRAIIRECRNTFSCFFTNSRVEFIRRQANEVAHRLARVATFYASIHY*