AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040095.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 109 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTCCTCCCATAGTTATT+TGG 0.187096 7:+73002143 None:intergenic
TTCACTTCCTGGTGATCTAT+TGG 0.189914 7:+72999829 None:intergenic
TCCTTTAGCTGTTCACTTCC+TGG 0.216962 7:+72999818 None:intergenic
TTGTCGACAGAGCGTATTTC+AGG 0.217309 7:-73000660 MsG0780040095.01.T01:CDS
TCACTTCCTGGTGATCTATT+GGG 0.222828 7:+72999830 None:intergenic
TCATACTCAAACTGAATATC+TGG 0.288502 7:+73000761 None:intergenic
GCTCATACTCCAAATAACTA+TGG 0.290254 7:-73002152 MsG0780040095.01.T01:CDS
TGAAGAGTACTCGTTTGTTT+TGG 0.318646 7:+72999929 None:intergenic
TCTCAATGATCTAGAGGGTT+TGG 0.330384 7:-72999753 MsG0780040095.01.T01:CDS
CGTTGAAGAAAGTTTAGATT+TGG 0.331795 7:+73002224 None:intergenic
GTTTCAATCATTTGATCCTA+TGG 0.343262 7:-72999957 MsG0780040095.01.T01:CDS
GAGAGATCTGCTTGCTTTGT+CGG 0.351865 7:+73000996 None:intergenic
TTCTTCAACGAATGCAGATA+TGG 0.362912 7:-73002211 MsG0780040095.01.T01:CDS
ATTTCTGCTGATGCATCATT+TGG 0.366884 7:-73000629 MsG0780040095.01.T01:intron
AAGTTTAGATTTGGAGGGTA+AGG 0.372144 7:+73002233 None:intergenic
CATCAATCATGCCAAGTCTA+TGG 0.378809 7:-72999624 MsG0780040095.01.T01:CDS
ATCCTGTGGTTACCGTGTTT+CGG 0.379808 7:-73001083 MsG0780040095.01.T01:CDS
CTTGAGAGAGACGTTGGAAT+TGG 0.387762 7:+73002419 None:intergenic
TGAAGAAGTCGCTAATCTTC+AGG 0.402805 7:-72999447 MsG0780040095.01.T01:CDS
ATTGGGGCTTACTACATTTG+TGG 0.412109 7:+72999847 None:intergenic
TCTTGTCCAATGGGTTGGTT+AGG 0.417836 7:-73000141 MsG0780040095.01.T01:CDS
ATTGCAGGTTCTTGTCCAAT+GGG 0.422632 7:-73000150 MsG0780040095.01.T01:intron
AGAGTTGAAATCCTGTGAAC+TGG 0.422824 7:-72999402 MsG0780040095.01.T01:CDS
GGAGCACATTAAATTCTTAG+AGG 0.431027 7:-72999561 MsG0780040095.01.T01:CDS
ATCTGCTTGCTTTGTCGGTA+AGG 0.434008 7:+73001001 None:intergenic
CTGTCGACAACTGGTCTTTG+AGG 0.436550 7:+73000673 None:intergenic
TATTGCAGGTTCTTGTCCAA+TGG 0.439287 7:-73000151 MsG0780040095.01.T01:intron
GTCAATTTCTGCCATAGACT+TGG 0.447175 7:+72999613 None:intergenic
TTACTACATTTGTGGAAAGC+TGG 0.448068 7:+72999855 None:intergenic
TAAACAATTTAACACTGAAA+AGG 0.453558 7:+72999688 None:intergenic
CATGGAAAATTCGCCTACTC+TGG 0.455767 7:-72999585 MsG0780040095.01.T01:CDS
CAGGTTCTTGTCCAATGGGT+TGG 0.456800 7:-73000146 MsG0780040095.01.T01:CDS
AGCGGTGATATTTATGCAGC+AGG 0.459863 7:-72999780 MsG0780040095.01.T01:CDS
ATGAGATGATAGAGAAGCTT+CGG 0.460418 7:+73000706 None:intergenic
TACTACATTTGTGGAAAGCT+GGG 0.461766 7:+72999856 None:intergenic
CACTTCCTGGTGATCTATTG+GGG 0.464562 7:+72999831 None:intergenic
AAATACGCTCTGTCGACAAC+TGG 0.468375 7:+73000664 None:intergenic
CTTTACATGTCCCATCAGAT+TGG 0.471970 7:-73002120 MsG0780040095.01.T01:intron
CTCTCTCAATGAATATATTG+AGG 0.476634 7:-73000978 MsG0780040095.01.T01:CDS
TGAAGAAAGTTTAGATTTGG+AGG 0.477260 7:+73002227 None:intergenic
GAAAGCTGGGTGCATTGATT+AGG 0.478504 7:+72999869 None:intergenic
AGAGGTGGAGAAAGTGAGAT+TGG 0.481512 7:-72999543 MsG0780040095.01.T01:CDS
AAGTGAGATTGGCTAACATC+CGG 0.482030 7:-72999532 MsG0780040095.01.T01:CDS
TGTTGGATGAAACTGAGAAT+GGG 0.485353 7:+73002274 None:intergenic
TGTCGACAACTGGTCTTTGA+GGG 0.487261 7:+73000674 None:intergenic
CCTCCATCTCTCCAGTTCAC+AGG 0.489516 7:+72999391 None:intergenic
TGTGAATTATCTTCCTCTGT+TGG 0.489986 7:+73000797 None:intergenic
TGAATATCTGGAAAACCGTC+AGG 0.499648 7:+73000773 None:intergenic
GATAGACTTAGTTGCAATTG+AGG 0.502431 7:-73000729 MsG0780040095.01.T01:CDS
ATATGGTGTCCTTGCGGTCA+TGG 0.508748 7:-73002194 MsG0780040095.01.T01:CDS
ACCAGGAAGTGAACAGCTAA+AGG 0.511344 7:-72999819 MsG0780040095.01.T01:CDS
TCTCTCAATGAATATATTGA+GGG 0.517423 7:-73000977 MsG0780040095.01.T01:CDS
TAGATTTGGAGGGTAAGGAG+TGG 0.525323 7:+73002238 None:intergenic
GTTTCGTTGTTCTTCAACAG+TGG 0.527464 7:+73002382 None:intergenic
GAAGAGCTTGAGAGAGACGT+TGG 0.531011 7:+73002413 None:intergenic
AGTGAGATTGGCTAACATCC+GGG 0.531581 7:-72999531 MsG0780040095.01.T01:CDS
CCTCAGCGAAGTCATTAGTC+AGG 0.536242 7:+73000927 None:intergenic
ACGATGCTTGTGTTATCGAC+TGG 0.536824 7:+73001025 None:intergenic
ATTGATATCAAACAGGGTCA+TGG 0.537624 7:-73001106 MsG0780040095.01.T01:intron
TGAAGGAGAATGAGGAATGA+AGG 0.538447 7:+73002298 None:intergenic
TTGTTGGATGAAACTGAGAA+TGG 0.539408 7:+73002273 None:intergenic
CGTTGCCAGCTGTATCAACT+TGG 0.539514 7:+73000951 None:intergenic
GCACATTAAATTCTTAGAGG+TGG 0.539719 7:-72999558 MsG0780040095.01.T01:CDS
CTCATACTCCAAATAACTAT+GGG 0.546444 7:-73002151 MsG0780040095.01.T01:CDS
CAATCTTCACACAGTCATCC+CGG 0.546682 7:+72999513 None:intergenic
TGTCTCTCATAGACTCGTGC+TGG 0.547080 7:+72999723 None:intergenic
TGTCGACAGAGCGTATTTCA+GGG 0.547872 7:-73000659 MsG0780040095.01.T01:CDS
GGCAGAAATTGACAAGTCCA+TGG 0.550972 7:-72999603 MsG0780040095.01.T01:CDS
CTAGTAACATACCAATCTGA+TGG 0.551204 7:+73002109 None:intergenic
GGAGACCAAGTTGATACAGC+TGG 0.551737 7:-73000956 MsG0780040095.01.T01:CDS
TCAGATACAGCCACAGGTGA+AGG 0.551842 7:+73000848 None:intergenic
GAAGAAAGTTTAGATTTGGA+GGG 0.553211 7:+73002228 None:intergenic
GAATGGGGTGAAGGAGAATG+AGG 0.553661 7:+73002290 None:intergenic
TTCCGAAACACGGTAACCAC+AGG 0.572644 7:+73001081 None:intergenic
GGGACTTCTCAATGATCTAG+AGG 0.573353 7:-72999759 MsG0780040095.01.T01:CDS
AGGCGACCTAACCAACCCAT+TGG 0.580465 7:+73000135 None:intergenic
GTAAGCCCCAATAGATCACC+AGG 0.584261 7:-72999836 MsG0780040095.01.T01:CDS
ACAATTTAACACTGAAAAGG+CGG 0.584452 7:+72999691 None:intergenic
CCTGTGAACTGGAGAGATGG+AGG 0.591770 7:-72999391 MsG0780040095.01.T01:CDS
TGAAACTGAGAATGGGGTGA+AGG 0.592296 7:+73002281 None:intergenic
TTCTGAGAATCCTTCACCTG+TGG 0.597324 7:-73000858 MsG0780040095.01.T01:CDS
GGAAAATTCGCCTACTCTGG+AGG 0.597939 7:-72999582 MsG0780040095.01.T01:CDS
GGAATGAAGGTGGTTTGTTG+TGG 0.603381 7:+73002311 None:intergenic
GGAATCTCAGATACAGCCAC+AGG 0.604606 7:+73000842 None:intergenic
GAGAGATGGAGGAAGAGACC+CGG 0.606987 7:-72999380 MsG0780040095.01.T01:CDS
TAGTAACATACCAATCTGAT+GGG 0.607557 7:+73002110 None:intergenic
AGACAGACACCATGACCGCA+AGG 0.613283 7:+73002185 None:intergenic
AATCCTGTGAACTGGAGAGA+TGG 0.614486 7:-72999394 MsG0780040095.01.T01:CDS
TTAATGTGCTCCTCCAGAGT+AGG 0.616939 7:+72999572 None:intergenic
AGGAGAATGAGGAATGAAGG+TGG 0.616950 7:+73002301 None:intergenic
GAAGTTCATCTTCCGAAACA+CGG 0.621723 7:+73001071 None:intergenic
ATAGTTATTTGGAGTATGAG+CGG 0.622038 7:+73002154 None:intergenic
GTCGACAGAGCGTATTTCAG+GGG 0.622969 7:-73000658 MsG0780040095.01.T01:CDS
ATACTCCAAATAACTATGGG+AGG 0.623595 7:-73002148 MsG0780040095.01.T01:CDS
CTGACTAATGACTTCGCTGA+GGG 0.625887 7:-73000926 MsG0780040095.01.T01:CDS
GGCTGTATCTGAGATTCCTG+AGG 0.627996 7:-73000837 MsG0780040095.01.T01:CDS
GGACTTCTCAATGATCTAGA+GGG 0.629522 7:-72999758 MsG0780040095.01.T01:CDS
AAACAGGGTCATGGATCCTG+TGG 0.637813 7:-73001097 MsG0780040095.01.T01:intron
GCGGTGATATTTATGCAGCA+GGG 0.640685 7:-72999779 MsG0780040095.01.T01:CDS
AAGCAGAGGATCACCAACAG+AGG 0.648823 7:-73000810 MsG0780040095.01.T01:CDS
AAAATAGCTTAGGTGTGTAG+GGG 0.650871 7:+73000080 None:intergenic
GAAGATAATTCACAACCTGA+CGG 0.651055 7:-73000788 MsG0780040095.01.T01:CDS
GTTGGATGAAACTGAGAATG+GGG 0.652588 7:+73002275 None:intergenic
GGAACGAAAACAAAGAGAAG+CGG 0.669686 7:-72999798 MsG0780040095.01.T01:CDS
TCGACAGAGCGTATTTCAGG+GGG 0.670022 7:-73000657 MsG0780040095.01.T01:CDS
ATGCAGATATGGTGTCCTTG+CGG 0.677815 7:-73002200 MsG0780040095.01.T01:CDS
GCTGTATCTGAGATTCCTGA+GGG 0.692469 7:-73000836 MsG0780040095.01.T01:CDS
CCTGACTAATGACTTCGCTG+AGG 0.704482 7:-73000927 MsG0780040095.01.T01:CDS
AAAAGGATGAGAATAACAGA+CGG 0.712163 7:-73000881 MsG0780040095.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTATTATGTTTTTTTG+TGG - Chr7:72999774-72999793 MsG0780040095.01.T01:CDS 10.0%
!! AAAAATGAGAAAAAAAAACA+TGG + Chr7:72999752-72999771 None:intergenic 15.0%
!! AATCTTATTGATATCAAACA+GGG - Chr7:73000683-73000702 MsG0780040095.01.T01:CDS 20.0%
!! ACATAAACAAAGTAGTTTAA+TGG + Chr7:73000052-73000071 None:intergenic 20.0%
!! TAAACAATTTAACACTGAAA+AGG + Chr7:73002110-73002129 None:intergenic 20.0%
!! TAATCTTATTGATATCAAAC+AGG - Chr7:73000682-73000701 MsG0780040095.01.T01:CDS 20.0%
!! TATGAAAAATCGAAACTTTA+AGG + Chr7:72999723-72999742 None:intergenic 20.0%
!!! ATCAAATTAATTTGAACCTA+AGG - Chr7:73001486-73001505 MsG0780040095.01.T01:intron 20.0%
!!! GAAGAGTAATTTATTTTTTG+TGG - Chr7:73000527-73000546 MsG0780040095.01.T01:intron 20.0%
! AAAACATCATACCTGTTTAA+TGG + Chr7:72999932-72999951 None:intergenic 25.0%
! AAACATCATACCTGTTTAAT+GGG + Chr7:72999931-72999950 None:intergenic 25.0%
! AAGTTGAACATTGCAAATAA+AGG + Chr7:73000391-73000410 None:intergenic 25.0%
! ATTCAAAATGACATAACTTG+CGG + Chr7:73001273-73001292 None:intergenic 25.0%
! GAGTTGGATGAAAAAAAATA+AGG + Chr7:73001683-73001702 None:intergenic 25.0%
! GTTTGTAAAAAATTGATGGA+AGG - Chr7:72999883-72999902 MsG0780040095.01.T01:CDS 25.0%
! GTTTGTTTAGTACCAAATAA+GGG - Chr7:73000014-73000033 MsG0780040095.01.T01:intron 25.0%
! TAATTTGAACCTAAGGAATT+TGG - Chr7:73001493-73001512 MsG0780040095.01.T01:intron 25.0%
! TACTAAGTAAAGCAATTGAT+TGG + Chr7:73001335-73001354 None:intergenic 25.0%
! TCTCTCAATGAATATATTGA+GGG - Chr7:73000818-73000837 MsG0780040095.01.T01:CDS 25.0%
! TGGAGTTTGTAAAAAATTGA+TGG - Chr7:72999879-72999898 MsG0780040095.01.T01:CDS 25.0%
! TGTTTGTTTAGTACCAAATA+AGG - Chr7:73000013-73000032 MsG0780040095.01.T01:intron 25.0%
!! AACAATTTTCTGACTATGTA+TGG - Chr7:73002143-73002162 MsG0780040095.01.T01:CDS 25.0%
!! AATTTTACCAATCGCAATTT+TGG + Chr7:73000213-73000232 None:intergenic 25.0%
!! ACAATTTTCTGACTATGTAT+GGG - Chr7:73002144-73002163 MsG0780040095.01.T01:CDS 25.0%
!! CTACTTTGTTTATGTATTGT+TGG - Chr7:73000057-73000076 MsG0780040095.01.T01:intron 25.0%
!! TACCAAATAAGGGTTTATTT+TGG - Chr7:73000024-73000043 MsG0780040095.01.T01:intron 25.0%
!! TAGTCACAAGTTTTTACAAA+AGG + Chr7:73000289-73000308 None:intergenic 25.0%
!! TTGGTACTATTTTCTTTTTG+TGG - Chr7:73001185-73001204 MsG0780040095.01.T01:intron 25.0%
!!! TTTTATTTGTCCCATTAAAC+AGG - Chr7:72999918-72999937 MsG0780040095.01.T01:CDS 25.0%
AAAAATAGCTTAGGTGTGTA+GGG + Chr7:73001719-73001738 None:intergenic 30.0%
AAAAGGATGAGAATAACAGA+CGG - Chr7:73000914-73000933 MsG0780040095.01.T01:CDS 30.0%
AAGAGAAGAAGAAGAAGAAA+TGG - Chr7:72999450-72999469 MsG0780040095.01.T01:CDS 30.0%
AATTCTCTTGATCTTATTGC+AGG - Chr7:73001630-73001649 MsG0780040095.01.T01:intron 30.0%
ACAATTTAACACTGAAAAGG+CGG + Chr7:73002107-73002126 None:intergenic 30.0%
AGAATAATTAAACGTGAGGT+GGG - Chr7:73000415-73000434 MsG0780040095.01.T01:intron 30.0%
AGAGAAGAAGAAGAAGAAAT+GGG - Chr7:72999451-72999470 MsG0780040095.01.T01:CDS 30.0%
ATAACACAACCAAATTCCTT+AGG + Chr7:73001505-73001524 None:intergenic 30.0%
ATCAAGAGAATTTCCACATA+TGG + Chr7:73001622-73001641 None:intergenic 30.0%
ATGTTCAGCTATAATGACTT+TGG + Chr7:73001382-73001401 None:intergenic 30.0%
ATTATGACCAAAATTGCGAT+TGG - Chr7:73000203-73000222 MsG0780040095.01.T01:intron 30.0%
ATTGGATGTATAGAGAAGAA+GGG + Chr7:73001301-73001320 None:intergenic 30.0%
ATTGTGATCACTGATCAATA+GGG - Chr7:73000477-73000496 MsG0780040095.01.T01:intron 30.0%
CACACAAACAAATGATATCA+AGG + Chr7:73001760-73001779 None:intergenic 30.0%
CACCAAAATAAACCCTTATT+TGG + Chr7:73000029-73000048 None:intergenic 30.0%
CGTTGAAGAAAGTTTAGATT+TGG + Chr7:72999574-72999593 None:intergenic 30.0%
CTCATACTCCAAATAACTAT+GGG - Chr7:72999644-72999663 MsG0780040095.01.T01:CDS 30.0%
CTCTCTCAATGAATATATTG+AGG - Chr7:73000817-73000836 MsG0780040095.01.T01:CDS 30.0%
GAAGAAAGTTTAGATTTGGA+GGG + Chr7:72999570-72999589 None:intergenic 30.0%
GTCATTATAGCTGAACATAT+AGG - Chr7:73001384-73001403 MsG0780040095.01.T01:intron 30.0%
GTTGAGAATAATTAAACGTG+AGG - Chr7:73000411-73000430 MsG0780040095.01.T01:intron 30.0%
GTTTCAATCATTTGATCCTA+TGG - Chr7:73001838-73001857 MsG0780040095.01.T01:intron 30.0%
TAACAGCAGAAAAATAGCTT+AGG + Chr7:73001728-73001747 None:intergenic 30.0%
TAGTAACATACCAATCTGAT+GGG + Chr7:72999688-72999707 None:intergenic 30.0%
TCATACTCAAACTGAATATC+TGG + Chr7:73001037-73001056 None:intergenic 30.0%
TGAAACATACCTGTAATGAT+GGG + Chr7:73001824-73001843 None:intergenic 30.0%
TGAAGAAAGTTTAGATTTGG+AGG + Chr7:72999571-72999590 None:intergenic 30.0%
TGTAAAAAATTGATGGAAGG+AGG - Chr7:72999886-72999905 MsG0780040095.01.T01:CDS 30.0%
TTGAAACATACCTGTAATGA+TGG + Chr7:73001825-73001844 None:intergenic 30.0%
! ATAGTTATTTGGAGTATGAG+CGG + Chr7:72999644-72999663 None:intergenic 30.0%
! GAAAAATCGAAACTTTAAGG+TGG + Chr7:72999720-72999739 None:intergenic 30.0%
! GATTTAAAACCTTGGTTAGT+GGG + Chr7:73000117-73000136 None:intergenic 30.0%
! GTGTTACATAGTTGTTATTG+CGG - Chr7:73000158-73000177 MsG0780040095.01.T01:intron 30.0%
! TGAATTGGATTTTCTGTTGA+TGG - Chr7:73000440-73000459 MsG0780040095.01.T01:intron 30.0%
! TTTTTGTGGCAATTGTAGTT+GGG - Chr7:73000541-73000560 MsG0780040095.01.T01:intron 30.0%
!! CTATTTTTCTGCTGTTAGTA+TGG - Chr7:73001731-73001750 MsG0780040095.01.T01:intron 30.0%
!! TTTTGCTATCAACGAACAAA+AGG - Chr7:73000897-73000916 MsG0780040095.01.T01:CDS 30.0%
!!! TTTTTTGTGGCAATTGTAGT+TGG - Chr7:73000540-73000559 MsG0780040095.01.T01:intron 30.0%
AAAATAGCTTAGGTGTGTAG+GGG + Chr7:73001718-73001737 None:intergenic 35.0%
AACTTCCTCCCATAGTTATT+TGG + Chr7:72999655-72999674 None:intergenic 35.0%
AAGTTTAGATTTGGAGGGTA+AGG + Chr7:72999565-72999584 None:intergenic 35.0%
AATAATTAAACGTGAGGTGG+GGG - Chr7:73000417-73000436 MsG0780040095.01.T01:intron 35.0%
ATAACAACTATGTAACACCG+AGG + Chr7:73000156-73000175 None:intergenic 35.0%
ATACTCCAAATAACTATGGG+AGG - Chr7:72999647-72999666 MsG0780040095.01.T01:CDS 35.0%
ATGAGATGATAGAGAAGCTT+CGG + Chr7:73001092-73001111 None:intergenic 35.0%
ATTGATATCAAACAGGGTCA+TGG - Chr7:73000689-73000708 MsG0780040095.01.T01:CDS 35.0%
ATTTCTGCTGATGCATCATT+TGG - Chr7:73001166-73001185 MsG0780040095.01.T01:intron 35.0%
CACTCAGGATAAGTAAACAA+AGG + Chr7:72999847-72999866 None:intergenic 35.0%
CTAGTAACATACCAATCTGA+TGG + Chr7:72999689-72999708 None:intergenic 35.0%
GAAAAATAGCTTAGGTGTGT+AGG + Chr7:73001720-73001739 None:intergenic 35.0%
GAAGATAATTCACAACCTGA+CGG - Chr7:73001007-73001026 MsG0780040095.01.T01:CDS 35.0%
GAATAATTAAACGTGAGGTG+GGG - Chr7:73000416-73000435 MsG0780040095.01.T01:intron 35.0%
GAGAAGAAGAAGAAGAAATG+GGG - Chr7:72999452-72999471 MsG0780040095.01.T01:CDS 35.0%
GAGAATAATTAAACGTGAGG+TGG - Chr7:73000414-73000433 MsG0780040095.01.T01:intron 35.0%
GATAGACTTAGTTGCAATTG+AGG - Chr7:73001066-73001085 MsG0780040095.01.T01:CDS 35.0%
GATTGGATGTATAGAGAAGA+AGG + Chr7:73001302-73001321 None:intergenic 35.0%
GATTGTGATCACTGATCAAT+AGG - Chr7:73000476-73000495 MsG0780040095.01.T01:intron 35.0%
GCACATTAAATTCTTAGAGG+TGG - Chr7:73002237-73002256 MsG0780040095.01.T01:CDS 35.0%
GCTCATACTCCAAATAACTA+TGG - Chr7:72999643-72999662 MsG0780040095.01.T01:CDS 35.0%
GGAGCACATTAAATTCTTAG+AGG - Chr7:73002234-73002253 MsG0780040095.01.T01:CDS 35.0%
GTAACTCGAAATGACGTTTA+AGG + Chr7:73001465-73001484 None:intergenic 35.0%
TACGCATAATGAATAGATCC+TGG + Chr7:73000658-73000677 None:intergenic 35.0%
TACTACATTTGTGGAAAGCT+GGG + Chr7:73001942-73001961 None:intergenic 35.0%
TGAATAATGCAAAGCCAAAG+AGG + Chr7:73001787-73001806 None:intergenic 35.0%
TGCAAATAAAGGACTAAGCT+TGG + Chr7:73000380-73000399 None:intergenic 35.0%
TGTGAATTATCTTCCTCTGT+TGG + Chr7:73001001-73001020 None:intergenic 35.0%
TGTTGGATGAAACTGAGAAT+GGG + Chr7:72999524-72999543 None:intergenic 35.0%
TTACTACATTTGTGGAAAGC+TGG + Chr7:73001943-73001962 None:intergenic 35.0%
TTCTTCAACGAATGCAGATA+TGG - Chr7:72999584-72999603 MsG0780040095.01.T01:CDS 35.0%
TTGTTGGATGAAACTGAGAA+TGG + Chr7:72999525-72999544 None:intergenic 35.0%
TTTAGTAGAGAAGCCATATG+TGG - Chr7:73001606-73001625 MsG0780040095.01.T01:intron 35.0%
! CGATTTAAAACCTTGGTTAG+TGG + Chr7:73000118-73000137 None:intergenic 35.0%
! TTTTTGTCTCCCATCATTAC+AGG - Chr7:73001812-73001831 MsG0780040095.01.T01:intron 35.0%
!! GGGGATTTCAAATTTTGAGT+TGG + Chr7:73001699-73001718 None:intergenic 35.0%
!!! CTAACCAAGGTTTTAAATCG+CGG - Chr7:73000118-73000137 MsG0780040095.01.T01:CDS 35.0%
!!! GTTTGTTTTGGAACATCCAT+AGG + Chr7:73001857-73001876 None:intergenic 35.0%
!!! TAGATTTGTTTTGGTCTACC+AGG - Chr7:73000637-73000656 MsG0780040095.01.T01:CDS 35.0%
!!! TGAAGAGTACTCGTTTGTTT+TGG + Chr7:73001869-73001888 None:intergenic 35.0%
!!! TTTTTGTTTTCGAATGAGCG+CGG + Chr7:73002374-73002393 None:intergenic 35.0%
AGAGTTGAAATCCTGTGAAC+TGG - Chr7:73002393-73002412 MsG0780040095.01.T01:CDS 40.0%
AGCAATTGATTGGAAAGACC+TGG + Chr7:73001325-73001344 None:intergenic 40.0%
ATTGCAGGTTCTTGTCCAAT+GGG - Chr7:73001645-73001664 MsG0780040095.01.T01:intron 40.0%
ATTGGGGCTTACTACATTTG+TGG + Chr7:73001951-73001970 None:intergenic 40.0%
CATCAATCATGCCAAGTCTA+TGG - Chr7:73002171-73002190 MsG0780040095.01.T01:CDS 40.0%
CCAAAACAAATCTACAGAGC+AGG + Chr7:73000631-73000650 None:intergenic 40.0%
CTTTACATGTCCCATCAGAT+TGG - Chr7:72999675-72999694 MsG0780040095.01.T01:CDS 40.0%
GAAGTTCATCTTCCGAAACA+CGG + Chr7:73000727-73000746 None:intergenic 40.0%
GACAGTAGTATAAGCACATG+TGG - Chr7:73001570-73001589 MsG0780040095.01.T01:intron 40.0%
GCAATTGATTGGAAAGACCT+GGG + Chr7:73001324-73001343 None:intergenic 40.0%
GGAACGAAAACAAAGAGAAG+CGG - Chr7:73001997-73002016 MsG0780040095.01.T01:intron 40.0%
GGACTTCTCAATGATCTAGA+GGG - Chr7:73002037-73002056 MsG0780040095.01.T01:intron 40.0%
GTCAATTTCTGCCATAGACT+TGG + Chr7:73002185-73002204 None:intergenic 40.0%
GTTGGATGAAACTGAGAATG+GGG + Chr7:72999523-72999542 None:intergenic 40.0%
GTTTCGTTGTTCTTCAACAG+TGG + Chr7:72999416-72999435 None:intergenic 40.0%
TATTGCAGGTTCTTGTCCAA+TGG - Chr7:73001644-73001663 MsG0780040095.01.T01:intron 40.0%
TCACAATGTCGCAATCTAGA+CGG - Chr7:73000253-73000272 MsG0780040095.01.T01:intron 40.0%
TCACTTCCTGGTGATCTATT+GGG + Chr7:73001968-73001987 None:intergenic 40.0%
TCTCAATGATCTAGAGGGTT+TGG - Chr7:73002042-73002061 MsG0780040095.01.T01:intron 40.0%
TGAAGAAGTCGCTAATCTTC+AGG - Chr7:73002348-73002367 MsG0780040095.01.T01:CDS 40.0%
TGAAGGAGAATGAGGAATGA+AGG + Chr7:72999500-72999519 None:intergenic 40.0%
TGAATATCTGGAAAACCGTC+AGG + Chr7:73001025-73001044 None:intergenic 40.0%
TGTTTGTGTGATGTCCTCTT+TGG - Chr7:73001770-73001789 MsG0780040095.01.T01:intron 40.0%
TTCACTTCCTGGTGATCTAT+TGG + Chr7:73001969-73001988 None:intergenic 40.0%
TTCTCTATACATCCAATCCC+AGG - Chr7:73001304-73001323 MsG0780040095.01.T01:intron 40.0%
TTGCAAATCAGTCTCATGGA+TGG + Chr7:72999821-72999840 None:intergenic 40.0%
! AAGTGAGATTGGCTAACATC+CGG - Chr7:73002263-73002282 MsG0780040095.01.T01:CDS 40.0%
! ATGACCGCGATTTAAAACCT+TGG + Chr7:73000125-73000144 None:intergenic 40.0%
! GTTGTTATTGCGGAGAATTC+TGG - Chr7:73000168-73000187 MsG0780040095.01.T01:intron 40.0%
!! CCTGCTCTGTAGATTTGTTT+TGG - Chr7:73000628-73000647 MsG0780040095.01.T01:intron 40.0%
!! GGTAAGGAGTGGATTTTTCA+GGG + Chr7:72999549-72999568 None:intergenic 40.0%
!! GTAAGGAGTGGATTTTTCAG+GGG + Chr7:72999548-72999567 None:intergenic 40.0%
!! TCGAAACTTTAAGGTGGTAC+TGG + Chr7:72999714-72999733 None:intergenic 40.0%
!! TTTTTAGCACCCACTAACCA+AGG - Chr7:73000105-73000124 MsG0780040095.01.T01:CDS 40.0%
AAATACGCTCTGTCGACAAC+TGG + Chr7:73001134-73001153 None:intergenic 45.0%
AATCCTGTGAACTGGAGAGA+TGG - Chr7:73002401-73002420 MsG0780040095.01.T01:CDS 45.0%
ACCAGGAAGTGAACAGCTAA+AGG - Chr7:73001976-73001995 MsG0780040095.01.T01:intron 45.0%
ACGATGCTTGTGTTATCGAC+TGG + Chr7:73000773-73000792 None:intergenic 45.0%
ACTAAGCTTGGCATGTGTCT+AGG + Chr7:73000368-73000387 None:intergenic 45.0%
AGAGGTGGAGAAAGTGAGAT+TGG - Chr7:73002252-73002271 MsG0780040095.01.T01:CDS 45.0%
AGCGGTGATATTTATGCAGC+AGG - Chr7:73002015-73002034 MsG0780040095.01.T01:intron 45.0%
AGGAGAATGAGGAATGAAGG+TGG + Chr7:72999497-72999516 None:intergenic 45.0%
ATCCTGTGGTTACCGTGTTT+CGG - Chr7:73000712-73000731 MsG0780040095.01.T01:CDS 45.0%
ATCTGCTTGCTTTGTCGGTA+AGG + Chr7:73000797-73000816 None:intergenic 45.0%
ATGCAGATATGGTGTCCTTG+CGG - Chr7:72999595-72999614 MsG0780040095.01.T01:CDS 45.0%
ATTCCTGAGGGAAAAAGCAG+AGG - Chr7:73000971-73000990 MsG0780040095.01.T01:CDS 45.0%
CAATCTTCACACAGTCATCC+CGG + Chr7:73002285-73002304 None:intergenic 45.0%
CACTTCCTGGTGATCTATTG+GGG + Chr7:73001967-73001986 None:intergenic 45.0%
CATGGAAAATTCGCCTACTC+TGG - Chr7:73002210-73002229 MsG0780040095.01.T01:CDS 45.0%
CTAAGCTTGGCATGTGTCTA+GGG + Chr7:73000367-73000386 None:intergenic 45.0%
CTGACTAATGACTTCGCTGA+GGG - Chr7:73000869-73000888 MsG0780040095.01.T01:CDS 45.0%
CTTGAGAGAGACGTTGGAAT+TGG + Chr7:72999379-72999398 None:intergenic 45.0%
GACGTTGCAAATCAGTCTCA+TGG + Chr7:72999825-72999844 None:intergenic 45.0%
GAGAGATCTGCTTGCTTTGT+CGG + Chr7:73000802-73000821 None:intergenic 45.0%
GCGGTGATATTTATGCAGCA+GGG - Chr7:73002016-73002035 MsG0780040095.01.T01:intron 45.0%
GGCAGAAATTGACAAGTCCA+TGG - Chr7:73002192-73002211 MsG0780040095.01.T01:CDS 45.0%
GGGACTTCTCAATGATCTAG+AGG - Chr7:73002036-73002055 MsG0780040095.01.T01:intron 45.0%
TAGATTTGGAGGGTAAGGAG+TGG + Chr7:72999560-72999579 None:intergenic 45.0%
TCCTTTAGCTGTTCACTTCC+TGG + Chr7:73001980-73001999 None:intergenic 45.0%
TGAAACTGAGAATGGGGTGA+AGG + Chr7:72999517-72999536 None:intergenic 45.0%
TGATTGGAAAGACCTGGGAT+TGG + Chr7:73001319-73001338 None:intergenic 45.0%
TGTCGACAGAGCGTATTTCA+GGG - Chr7:73001136-73001155 MsG0780040095.01.T01:intron 45.0%
TTAATGTGCTCCTCCAGAGT+AGG + Chr7:73002226-73002245 None:intergenic 45.0%
TTGTCGACAGAGCGTATTTC+AGG - Chr7:73001135-73001154 MsG0780040095.01.T01:intron 45.0%
! AGTGAGATTGGCTAACATCC+GGG - Chr7:73002264-73002283 MsG0780040095.01.T01:CDS 45.0%
! CAGAGTAGGCGAATTTTCCA+TGG + Chr7:73002212-73002231 None:intergenic 45.0%
! GCTGTATCTGAGATTCCTGA+GGG - Chr7:73000959-73000978 MsG0780040095.01.T01:CDS 45.0%
! TTCTGAGAATCCTTCACCTG+TGG - Chr7:73000937-73000956 MsG0780040095.01.T01:CDS 45.0%
!! GAAAGCTGGGTGCATTGATT+AGG + Chr7:73001929-73001948 None:intergenic 45.0%
!! GGAATGAAGGTGGTTTGTTG+TGG + Chr7:72999487-72999506 None:intergenic 45.0%
!! GGGTAAGGAGTGGATTTTTC+AGG + Chr7:72999550-72999569 None:intergenic 45.0%
!! TCTTGTCCAATGGGTTGGTT+AGG - Chr7:73001654-73001673 MsG0780040095.01.T01:intron 45.0%
!! TGTCGACAACTGGTCTTTGA+GGG + Chr7:73001124-73001143 None:intergenic 45.0%
!!! GTGGATTTTTCAGGGGTTGT+TGG + Chr7:72999541-72999560 None:intergenic 45.0%
AAACAGGGTCATGGATCCTG+TGG - Chr7:73000698-73000717 MsG0780040095.01.T01:CDS 50.0%
AAGCAGAGGATCACCAACAG+AGG - Chr7:73000985-73001004 MsG0780040095.01.T01:CDS 50.0%
ATATGGTGTCCTTGCGGTCA+TGG - Chr7:72999601-72999620 MsG0780040095.01.T01:CDS 50.0%
CAATGTCGCAATCTAGACGG+CGG - Chr7:73000256-73000275 MsG0780040095.01.T01:intron 50.0%
CAGGTTCTTGTCCAATGGGT+TGG - Chr7:73001649-73001668 MsG0780040095.01.T01:intron 50.0%
CCAGCAAGACATTGACACTC+AGG + Chr7:72999862-72999881 None:intergenic 50.0%
CCTCAGCGAAGTCATTAGTC+AGG + Chr7:73000871-73000890 None:intergenic 50.0%
CCTGACTAATGACTTCGCTG+AGG - Chr7:73000868-73000887 MsG0780040095.01.T01:CDS 50.0%
CCTGAGTGTCAATGTCTTGC+TGG - Chr7:72999859-72999878 MsG0780040095.01.T01:CDS 50.0%
CGTTGCCAGCTGTATCAACT+TGG + Chr7:73000847-73000866 None:intergenic 50.0%
CTTGCGGTGAAAGTAGAAGC+AGG + Chr7:73001257-73001276 None:intergenic 50.0%
GAAGAGCTTGAGAGAGACGT+TGG + Chr7:72999385-72999404 None:intergenic 50.0%
GAATGGGGTGAAGGAGAATG+AGG + Chr7:72999508-72999527 None:intergenic 50.0%
GGAAAATTCGCCTACTCTGG+AGG - Chr7:73002213-73002232 MsG0780040095.01.T01:CDS 50.0%
GGAATCTCAGATACAGCCAC+AGG + Chr7:73000956-73000975 None:intergenic 50.0%
GTAAGCCCCAATAGATCACC+AGG - Chr7:73001959-73001978 MsG0780040095.01.T01:intron 50.0%
GTCGACAGAGCGTATTTCAG+GGG - Chr7:73001137-73001156 MsG0780040095.01.T01:intron 50.0%
TCAGATACAGCCACAGGTGA+AGG + Chr7:73000950-73000969 None:intergenic 50.0%
TCGACAGAGCGTATTTCAGG+GGG - Chr7:73001138-73001157 MsG0780040095.01.T01:intron 50.0%
TGTCTCTCATAGACTCGTGC+TGG + Chr7:73002075-73002094 None:intergenic 50.0%
TTCCGAAACACGGTAACCAC+AGG + Chr7:73000717-73000736 None:intergenic 50.0%
! GGCTGTATCTGAGATTCCTG+AGG - Chr7:73000958-73000977 MsG0780040095.01.T01:CDS 50.0%
!! CTGTCGACAACTGGTCTTTG+AGG + Chr7:73001125-73001144 None:intergenic 50.0%
!! GGAGACCAAGTTGATACAGC+TGG - Chr7:73000839-73000858 MsG0780040095.01.T01:CDS 50.0%
!!! GATCCTCTGCTTTTTCCCTC+AGG + Chr7:73000977-73000996 None:intergenic 50.0%
AACGTGAGGTGGGGGTGAAT+TGG - Chr7:73000425-73000444 MsG0780040095.01.T01:intron 55.0%
AGACAGACACCATGACCGCA+AGG + Chr7:72999613-72999632 None:intergenic 55.0%
AGGCGACCTAACCAACCCAT+TGG + Chr7:73001663-73001682 None:intergenic 55.0%
CCTCCATCTCTCCAGTTCAC+AGG + Chr7:73002407-73002426 None:intergenic 55.0%
CCTGTGAACTGGAGAGATGG+AGG - Chr7:73002404-73002423 MsG0780040095.01.T01:CDS 55.0%
CGCGGTCATGTTGAGATCCT+CGG - Chr7:73000136-73000155 MsG0780040095.01.T01:CDS 55.0%
GAGAGATGGAGGAAGAGACC+CGG - Chr7:73002415-73002434 MsG0780040095.01.T01:CDS 55.0%
CGCAATCTAGACGGCGGTTC+TGG - Chr7:73000262-73000281 MsG0780040095.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 72999367 73002450 72999367 ID=MsG0780040095.01;Name=MsG0780040095.01
Chr7 mRNA 72999367 73002450 72999367 ID=MsG0780040095.01.T01;Parent=MsG0780040095.01;Name=MsG0780040095.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|4|0|508
Chr7 exon 73002121 73002450 73002121 ID=MsG0780040095.01.T01:exon:13077;Parent=MsG0780040095.01.T01
Chr7 exon 73000630 73001113 73000630 ID=MsG0780040095.01.T01:exon:13076;Parent=MsG0780040095.01.T01
Chr7 exon 73000070 73000165 73000070 ID=MsG0780040095.01.T01:exon:13075;Parent=MsG0780040095.01.T01
Chr7 exon 72999367 72999983 72999367 ID=MsG0780040095.01.T01:exon:13074;Parent=MsG0780040095.01.T01
Chr7 CDS 73002121 73002450 73002121 ID=MsG0780040095.01.T01:cds;Parent=MsG0780040095.01.T01
Chr7 CDS 73000630 73001113 73000630 ID=MsG0780040095.01.T01:cds;Parent=MsG0780040095.01.T01
Chr7 CDS 73000070 73000165 73000070 ID=MsG0780040095.01.T01:cds;Parent=MsG0780040095.01.T01
Chr7 CDS 72999367 72999983 72999367 ID=MsG0780040095.01.T01:cds;Parent=MsG0780040095.01.T01
Gene Sequence

>MsG0780040095.01.T01

ATGATCACACCAATTCCAACGTCTCTCTCAAGCTCTTCTTCATTTTCCACTGTTGAAGAACAACGAAACAATCAGAAAAGAAAAAGAGAAGAAGAAGAAGAAATGGGGTGTGAAGAACCACAACAAACCACCTTCATTCCTCATTCTCCTTCACCCCATTCTCAGTTTCATCCAACAACCCCTGAAAAATCCACTCCTTACCCTCCAAATCTAAACTTTCTTCAACGAATGCAGATATGGTGTCCTTGCGGTCATGGTGTCTGTCTTCTCAAAACCGCTCATACTCCAAATAACTATGGGAGGAAGTTCTTTACATGTCCCATCAGATTGGGTCATGGATCCTGTGGTTACCGTGTTTCGGAAGATGAACTTCTCAATAATGCAAGCATTGATGAACCAGTCGATAACACAAGCATCGTACCTTACCGACAAAGCAAGCAGATCTCTCTCAATGAATATATTGAGGGAGACCAAGTTGATACAGCTGGCAACGACCTGACTAATGACTTCGCTGAGGGCAGTGTTTTGCTATCAACGAACAAAAGGATGAGAATAACAGACGGTTCTGAGAATCCTTCACCTGTGGCTGTATCTGAGATTCCTGAGGGAAAAAGCAGAGGATCACCAACAGAGGAAGATAATTCACAACCTGACGGTTTTCCAGATATTCAGTTTGAGTATGATCTAGAATCGATAGACTTAGTTGCAATTGAGGCCGAAGCTTCTCTATCATCTCATTTTTCAACACCCTCAAAGACCAGTTGTCGACAGAGCGTATTTCAGGGGGACATTATTTCTGCTGATGCATCATTTGGTTCTTGTCCAATGGGTTGGTTAGGTCGCCTTATTTTTTTTCATCCAACTCAAAATTTGAAATCCCCTACACACCTAAGCTATTTTTCTGCTGTTAGTATGTTTCAATCATTTGATCCTATGGATGTTCCAAAACAAACGAGTACTCTTCATAGTCAAAGTGAATGCAACGATCTCGTTATCAAGAGTCCTAATCAATGCACCCAGCTTTCCACAAATGTAGTAAGCCCCAATAGATCACCAGGAAGTGAACAGCTAAAGGAACGAAAACAAAGAGAAGCGGTGATATTTATGCAGCAGGGACTTCTCAATGATCTAGAGGGTTTGGATTTTCACCAGCACGAGTCTATGAGAGACACAGCAGAAGCCGCCTTTTCAGTGTTAAATTGTTTAAGATTTGATTATAAACAATTTTCTGACTATGTATGGGAGTTCATCAATCATGCCAAGTCTATGGCAGAAATTGACAAGTCCATGGAAAATTCGCCTACTCTGGAGGAGCACATTAAATTCTTAGAGGTGGAGAAAGTGAGATTGGCTAACATCCGGGATGACTGTGTGAAGATTGAGACTTCGCTCGCAGTATTTGATGAGAAAAGAAATTTGCTTAGTGAAGAAGTCGCTAATCTTCAGGCCGCGCTCATTCGAAAACAAAAAGAGTTGAAATCCTGTGAACTGGAGAGATGGAGGAAGAGACCCGGCTTGACGATATGA
Protein sequence

>MsG0780040095.01.T01

MITPIPTSLSSSSSFSTVEEQRNNQKRKREEEEEMGCEEPQQTTFIPHSPSPHSQFHPTTPEKSTPYPPNLNFLQRMQIWCPCGHGVCLLKTAHTPNNYGRKFFTCPIRLGHGSCGYRVSEDELLNNASIDEPVDNTSIVPYRQSKQISLNEYIEGDQVDTAGNDLTNDFAEGSVLLSTNKRMRITDGSENPSPVAVSEIPEGKSRGSPTEEDNSQPDGFPDIQFEYDLESIDLVAIEAEASLSSHFSTPSKTSCRQSVFQGDIISADASFGSCPMGWLGRLIFFHPTQNLKSPTHLSYFSAVSMFQSFDPMDVPKQTSTLHSQSECNDLVIKSPNQCTQLSTNVVSPNRSPGSEQLKERKQREAVIFMQQGLLNDLEGLDFHQHESMRDTAEAAFSVLNCLRFDYKQFSDYVWEFINHAKSMAEIDKSMENSPTLEEHIKFLEVEKVRLANIRDDCVKIETSLAVFDEKRNLLSEEVANLQAALIRKQKELKSCELERWRKRPGLTI*