Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040099.01.T01 | XP_024625451.1 | 88.073 | 109 | 13 | 0 | 11 | 119 | 411 | 519 | 6.40E-58 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040099.01.T01 | A0A396H208 | 88.073 | 109 | 13 | 0 | 11 | 119 | 411 | 519 | 3.06e-58 | 199 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280009794.01 | MsG0780040099.01 | 0.813925 | 1.999316e-51 | 1.322971e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACTTCCTGGTGATCTAT+TGG | 0.189914 | 7:-73045380 | None:intergenic |
| TCCTTTAGCTGTTCACTTCC+TGG | 0.216962 | 7:-73045391 | None:intergenic |
| TCACTTCCTGGTGATCTATT+GGG | 0.271882 | 7:-73045379 | None:intergenic |
| TCTCAATGATCTAGAGGGTT+TGG | 0.330384 | 7:+73045456 | MsG0780040099.01.T01:CDS |
| CATCAATCATGCCAAGTCTA+TGG | 0.378809 | 7:+73045585 | MsG0780040099.01.T01:CDS |
| CTCCATCTTCTCCAGTTCAC+AGG | 0.397942 | 7:-73046350 | None:intergenic |
| TGAAGAAGTCGCTAATCTTC+AGG | 0.402805 | 7:+73046294 | MsG0780040099.01.T01:three_prime_UTR |
| TATTGGGCTTACTACATTTG+TGG | 0.412109 | 7:-73045363 | None:intergenic |
| AGAGTTGAAATCCTGTGAAC+TGG | 0.422824 | 7:+73046339 | MsG0780040099.01.T01:three_prime_UTR |
| GGAGCACATTAAATTCTTAG+AGG | 0.431027 | 7:+73045648 | MsG0780040099.01.T01:CDS |
| GGAGCACATTAAATTCTTAG+AGG | 0.431027 | 7:+73046180 | MsG0780040099.01.T01:three_prime_UTR |
| GTCAATTTCTGCCATAGACT+TGG | 0.447175 | 7:-73045596 | None:intergenic |
| TTACTACATTTGTGGAAAGC+TGG | 0.448068 | 7:-73045355 | None:intergenic |
| TAAACAATTTAACACTGAAA+AGG | 0.453558 | 7:-73045521 | None:intergenic |
| CATGGAAAATTCGCCTACTC+TGG | 0.455767 | 7:+73045624 | MsG0780040099.01.T01:CDS |
| CATGGAAAATTCGCCTACTC+TGG | 0.455767 | 7:+73046156 | MsG0780040099.01.T01:three_prime_UTR |
| AGCGGTGATATTTATGCAGC+AGG | 0.459863 | 7:+73045429 | MsG0780040099.01.T01:CDS |
| TACTACATTTGTGGAAAGCT+GGG | 0.461766 | 7:-73045354 | None:intergenic |
| AGAGGTGGAGAAAGTGAGAT+TGG | 0.481512 | 7:+73045666 | MsG0780040099.01.T01:CDS |
| AGAGGTGGAGAAAGTGAGAT+TGG | 0.481512 | 7:+73046198 | MsG0780040099.01.T01:three_prime_UTR |
| AAGTGAGATTGGCTAACATC+CGG | 0.482030 | 7:+73045677 | MsG0780040099.01.T01:CDS |
| AAGTGAGATTGGCTAACATC+CGG | 0.482030 | 7:+73046209 | MsG0780040099.01.T01:three_prime_UTR |
| ACCAGGAAGTGAACAGCTAA+AGG | 0.511344 | 7:+73045390 | MsG0780040099.01.T01:CDS |
| ATCCTGTGAACTGGAGAAGA+TGG | 0.523213 | 7:+73046348 | MsG0780040099.01.T01:three_prime_UTR |
| AGTGAGATTGGCTAACATCC+GGG | 0.531581 | 7:+73045678 | MsG0780040099.01.T01:CDS |
| AGTGAGATTGGCTAACATCC+GGG | 0.531581 | 7:+73046210 | MsG0780040099.01.T01:three_prime_UTR |
| GCACATTAAATTCTTAGAGG+TGG | 0.539719 | 7:+73045651 | MsG0780040099.01.T01:CDS |
| GCACATTAAATTCTTAGAGG+TGG | 0.539719 | 7:+73046183 | MsG0780040099.01.T01:three_prime_UTR |
| CAATCTTCACACAGTCATCC+CGG | 0.546682 | 7:-73046228 | None:intergenic |
| GGCAGAAATTGACAAGTCCA+TGG | 0.550972 | 7:+73045606 | MsG0780040099.01.T01:CDS |
| TCATCTTCACACAGTCATCC+CGG | 0.563085 | 7:-73045696 | None:intergenic |
| GGCAGAAATTGACAAGTCCA+TGG | 0.563627 | 7:+73046138 | MsG0780040099.01.T01:intron |
| GTCTCTCAATAGACTCGTGC+TGG | 0.570881 | 7:-73045485 | None:intergenic |
| AGTAAGCCCAATAGATCACC+AGG | 0.573107 | 7:+73045373 | MsG0780040099.01.T01:CDS |
| GGGACTTCTCAATGATCTAG+AGG | 0.573353 | 7:+73045450 | MsG0780040099.01.T01:CDS |
| ACAATTTAACACTGAAAAGG+CGG | 0.584452 | 7:-73045518 | None:intergenic |
| CGATATGAAAAGAAGGATGT+CGG | 0.589160 | 7:+73046390 | MsG0780040099.01.T01:three_prime_UTR |
| GGCTTGACGATATGAAAAGA+AGG | 0.596025 | 7:+73046383 | MsG0780040099.01.T01:three_prime_UTR |
| GGAAAATTCGCCTACTCTGG+AGG | 0.597939 | 7:+73045627 | MsG0780040099.01.T01:CDS |
| GGAAAATTCGCCTACTCTGG+AGG | 0.597939 | 7:+73046159 | MsG0780040099.01.T01:three_prime_UTR |
| AGAAGATGGAGGAAGAGACC+CGG | 0.604595 | 7:+73046362 | MsG0780040099.01.T01:three_prime_UTR |
| TTAATGTGCTCCTCCAGAGT+AGG | 0.616939 | 7:-73045637 | None:intergenic |
| TTAATGTGCTCCTCCAGAGT+AGG | 0.616939 | 7:-73046169 | None:intergenic |
| GGACTTCTCAATGATCTAGA+GGG | 0.629522 | 7:+73045451 | MsG0780040099.01.T01:CDS |
| GCGGTGATATTTATGCAGCA+GGG | 0.640685 | 7:+73045430 | MsG0780040099.01.T01:CDS |
| CTGTGAACTGGAGAAGATGG+AGG | 0.646229 | 7:+73046351 | MsG0780040099.01.T01:three_prime_UTR |
| GGAACGAAAACAAAGAGAAG+CGG | 0.669686 | 7:+73045411 | MsG0780040099.01.T01:CDS |
| TATGAAAAGAAGGATGTCGG+AGG | 0.720927 | 7:+73046393 | MsG0780040099.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACAATTTAACACTGAAA+AGG | - | Chr7:73045524-73045543 | None:intergenic | 20.0% |
| ! | CCAAATAACACATTATGATA+AGG | - | Chr7:73046117-73046136 | None:intergenic | 25.0% |
| !! | AACAATTTTCTGACTATGTA+TGG | + | Chr7:73045557-73045576 | MsG0780040099.01.T01:CDS | 25.0% |
| !! | ACAATTTTCTGACTATGTAT+GGG | + | Chr7:73045558-73045577 | MsG0780040099.01.T01:CDS | 25.0% |
| !! | CCTTATCATAATGTGTTATT+TGG | + | Chr7:73046114-73046133 | MsG0780040099.01.T01:intron | 25.0% |
| !!! | TATCATAATGTGTTATTTGG+TGG | + | Chr7:73046117-73046136 | MsG0780040099.01.T01:intron | 25.0% |
| ACAATTTAACACTGAAAAGG+CGG | - | Chr7:73045521-73045540 | None:intergenic | 30.0% | |
| !! | CTTGTCGAGTTTTATCTTTT+AGG | - | Chr7:73045896-73045915 | None:intergenic | 30.0% |
| CGATATGAAAAGAAGGATGT+CGG | + | Chr7:73045858-73045877 | MsG0780040099.01.T01:intron | 35.0% | |
| GCACATTAAATTCTTAGAGG+TGG | + | Chr7:73045651-73045670 | MsG0780040099.01.T01:CDS | 35.0% | |
| GGAGCACATTAAATTCTTAG+AGG | + | Chr7:73045648-73045667 | MsG0780040099.01.T01:CDS | 35.0% | |
| TACTACATTTGTGGAAAGCT+GGG | - | Chr7:73045357-73045376 | None:intergenic | 35.0% | |
| TATTGGGCTTACTACATTTG+TGG | - | Chr7:73045366-73045385 | None:intergenic | 35.0% | |
| TTACTACATTTGTGGAAAGC+TGG | - | Chr7:73045358-73045377 | None:intergenic | 35.0% | |
| ! | TTCTTTTCATATCGTCAAGC+CGG | - | Chr7:73045852-73045871 | None:intergenic | 35.0% |
| !!! | TTTTTGTTTTCGAATGAGCG+CGG | - | Chr7:73045788-73045807 | None:intergenic | 35.0% |
| AGAGTTGAAATCCTGTGAAC+TGG | + | Chr7:73045807-73045826 | MsG0780040099.01.T01:intron | 40.0% | |
| AGATTAACTCTGCATAGTGC+AGG | - | Chr7:73046093-73046112 | None:intergenic | 40.0% | |
| CATCAATCATGCCAAGTCTA+TGG | + | Chr7:73045585-73045604 | MsG0780040099.01.T01:CDS | 40.0% | |
| GATTAACTCTGCATAGTGCA+GGG | - | Chr7:73046092-73046111 | None:intergenic | 40.0% | |
| GGAACGAAAACAAAGAGAAG+CGG | + | Chr7:73045411-73045430 | MsG0780040099.01.T01:CDS | 40.0% | |
| GGACTTCTCAATGATCTAGA+GGG | + | Chr7:73045451-73045470 | MsG0780040099.01.T01:CDS | 40.0% | |
| GGCTTGACGATATGAAAAGA+AGG | + | Chr7:73045851-73045870 | MsG0780040099.01.T01:intron | 40.0% | |
| GTCAATTTCTGCCATAGACT+TGG | - | Chr7:73045599-73045618 | None:intergenic | 40.0% | |
| TAAAACTCGACAAGCAGAAG+CGG | + | Chr7:73045902-73045921 | MsG0780040099.01.T01:intron | 40.0% | |
| TATGAAAAGAAGGATGTCGG+AGG | + | Chr7:73045861-73045880 | MsG0780040099.01.T01:intron | 40.0% | |
| TCACTTCCTGGTGATCTATT+GGG | - | Chr7:73045382-73045401 | None:intergenic | 40.0% | |
| TCTCAATGATCTAGAGGGTT+TGG | + | Chr7:73045456-73045475 | MsG0780040099.01.T01:CDS | 40.0% | |
| TGAAGAAGTCGCTAATCTTC+AGG | + | Chr7:73045762-73045781 | MsG0780040099.01.T01:intron | 40.0% | |
| TTCACTTCCTGGTGATCTAT+TGG | - | Chr7:73045383-73045402 | None:intergenic | 40.0% | |
| ! | AAGTGAGATTGGCTAACATC+CGG | + | Chr7:73045677-73045696 | MsG0780040099.01.T01:CDS | 40.0% |
| ! | TCTTTTCATATCGTCAAGCC+GGG | - | Chr7:73045851-73045870 | None:intergenic | 40.0% |
| AACGTGCGTTGGAAAAGTAG+TGG | + | Chr7:73045992-73046011 | MsG0780040099.01.T01:intron | 45.0% | |
| ACCAGGAAGTGAACAGCTAA+AGG | + | Chr7:73045390-73045409 | MsG0780040099.01.T01:CDS | 45.0% | |
| AGAGGTGGAGAAAGTGAGAT+TGG | + | Chr7:73045666-73045685 | MsG0780040099.01.T01:CDS | 45.0% | |
| AGCGGTGATATTTATGCAGC+AGG | + | Chr7:73045429-73045448 | MsG0780040099.01.T01:CDS | 45.0% | |
| AGTAAGCCCAATAGATCACC+AGG | + | Chr7:73045373-73045392 | MsG0780040099.01.T01:CDS | 45.0% | |
| ATCCTGTGAACTGGAGAAGA+TGG | + | Chr7:73045816-73045835 | MsG0780040099.01.T01:intron | 45.0% | |
| CAATCTTCACACAGTCATCC+CGG | - | Chr7:73046231-73046250 | None:intergenic | 45.0% | |
| CATGGAAAATTCGCCTACTC+TGG | + | Chr7:73045624-73045643 | MsG0780040099.01.T01:CDS | 45.0% | |
| GCGGTGATATTTATGCAGCA+GGG | + | Chr7:73045430-73045449 | MsG0780040099.01.T01:CDS | 45.0% | |
| GGCAGAAATTGACAAGTCCA+TGG | + | Chr7:73045606-73045625 | MsG0780040099.01.T01:CDS | 45.0% | |
| GGGACTTCTCAATGATCTAG+AGG | + | Chr7:73045450-73045469 | MsG0780040099.01.T01:CDS | 45.0% | |
| TCATCTTCACACAGTCATCC+CGG | - | Chr7:73045699-73045718 | None:intergenic | 45.0% | |
| TCCTTTAGCTGTTCACTTCC+TGG | - | Chr7:73045394-73045413 | None:intergenic | 45.0% | |
| TTAATGTGCTCCTCCAGAGT+AGG | - | Chr7:73045640-73045659 | None:intergenic | 45.0% | |
| ! | AGTGAGATTGGCTAACATCC+GGG | + | Chr7:73045678-73045697 | MsG0780040099.01.T01:CDS | 45.0% |
| ! | CAGAGTAGGCGAATTTTCCA+TGG | - | Chr7:73045626-73045645 | None:intergenic | 45.0% |
| AGAAGATGGAGGAAGAGACC+CGG | + | Chr7:73045830-73045849 | MsG0780040099.01.T01:intron | 50.0% | |
| CTCCATCTTCTCCAGTTCAC+AGG | - | Chr7:73045821-73045840 | None:intergenic | 50.0% | |
| CTGTGAACTGGAGAAGATGG+AGG | + | Chr7:73045819-73045838 | MsG0780040099.01.T01:intron | 50.0% | |
| GGAAAATTCGCCTACTCTGG+AGG | + | Chr7:73045627-73045646 | MsG0780040099.01.T01:CDS | 50.0% | |
| GTCTCTCAATAGACTCGTGC+TGG | - | Chr7:73045488-73045507 | None:intergenic | 50.0% | |
| TAACTCTGCATAGTGCAGGG+TGG | - | Chr7:73046089-73046108 | None:intergenic | 50.0% | |
| AGCCTTCTCCAGTGCTGTCT+TGG | - | Chr7:73045971-73045990 | None:intergenic | 55.0% | |
| AGTTGCAGCCAAGACAGCAC+TGG | + | Chr7:73045960-73045979 | MsG0780040099.01.T01:intron | 55.0% | |
| GGAGAAGGCTAAACGTGCGT+TGG | + | Chr7:73045981-73046000 | MsG0780040099.01.T01:intron | 55.0% | |
| ! | AGCCAAGACAGCACTGGAGA+AGG | + | Chr7:73045966-73045985 | MsG0780040099.01.T01:intron | 55.0% |
| CTCGACAAGCAGAAGCGGCA+AGG | + | Chr7:73045907-73045926 | MsG0780040099.01.T01:intron | 60.0% | |
| CTCTGCATAGTGCAGGGTGG+TGG | - | Chr7:73046086-73046105 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 73045349 | 73046435 | 73045349 | ID=MsG0780040099.01;Name=MsG0780040099.01 |
| Chr7 | mRNA | 73045349 | 73046435 | 73045349 | ID=MsG0780040099.01.T01;Parent=MsG0780040099.01;Name=MsG0780040099.01.T01;_AED=0.50;_eAED=0.74;_QI=0|0|0|1|1|1|2|291|119 |
| Chr7 | exon | 73045349 | 73045706 | 73045349 | ID=MsG0780040099.01.T01:exon:13057;Parent=MsG0780040099.01.T01 |
| Chr7 | exon | 73046143 | 73046435 | 73046143 | ID=MsG0780040099.01.T01:exon:13058;Parent=MsG0780040099.01.T01 |
| Chr7 | CDS | 73045349 | 73045706 | 73045349 | ID=MsG0780040099.01.T01:cds;Parent=MsG0780040099.01.T01 |
| Chr7 | CDS | 73046143 | 73046144 | 73046143 | ID=MsG0780040099.01.T01:cds;Parent=MsG0780040099.01.T01 |
| Chr7 | three_prime_UTR | 73046145 | 73046435 | 73046145 | ID=MsG0780040099.01.T01:three_prime_utr;Parent=MsG0780040099.01.T01 |
| Gene Sequence |
| Protein sequence |
