Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040117.01.T01 | XP_003624546.1 | 91.126 | 293 | 15 | 1 | 1 | 282 | 1 | 293 | 0 | 550 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040117.01.T01 | Q8LNZ5 | 78.157 | 293 | 53 | 1 | 1 | 282 | 1 | 293 | 1.51E-176 | 491 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040117.01.T01 | Q2HRU7 | 91.126 | 293 | 15 | 1 | 1 | 282 | 1 | 293 | 0.0 | 550 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780040117.01 | MsG0780040119.01 | 0.862586 | 4.562179e-64 | 1.342976e-60 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880046024.01 | MsG0780040117.01 | PPI |
| MsG0880046025.01 | MsG0780040117.01 | PPI |
| MsG0880046024.01 | MsG0780040117.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040117.01.T01 | MTR_7g084750 | 91.126 | 293 | 15 | 1 | 1 | 282 | 1 | 293 | 0.0 | 550 |
| MsG0780040117.01.T01 | MTR_7g084770 | 79.863 | 293 | 48 | 1 | 1 | 282 | 1 | 293 | 1.58e-180 | 498 |
| MsG0780040117.01.T01 | MTR_7g084760 | 78.840 | 293 | 51 | 1 | 1 | 282 | 1 | 293 | 5.15e-178 | 492 |
| MsG0780040117.01.T01 | MTR_6g033055 | 73.379 | 293 | 67 | 1 | 1 | 282 | 1 | 293 | 1.77e-164 | 458 |
| MsG0780040117.01.T01 | MTR_6g033085 | 72.696 | 293 | 69 | 1 | 1 | 282 | 1 | 293 | 3.31e-164 | 457 |
| MsG0780040117.01.T01 | MTR_4g128410 | 48.454 | 291 | 126 | 5 | 1 | 277 | 1 | 281 | 7.89e-90 | 268 |
| MsG0780040117.01.T01 | MTR_2g089140 | 48.872 | 266 | 115 | 5 | 32 | 278 | 25 | 288 | 2.48e-88 | 265 |
| MsG0780040117.01.T01 | MTR_4g097700 | 48.249 | 257 | 120 | 3 | 32 | 276 | 69 | 324 | 8.51e-88 | 265 |
| MsG0780040117.01.T01 | MTR_4g097640 | 48.249 | 257 | 120 | 3 | 32 | 276 | 32 | 287 | 8.69e-88 | 263 |
| MsG0780040117.01.T01 | MTR_4g097670 | 48.249 | 257 | 120 | 3 | 32 | 276 | 32 | 287 | 8.69e-88 | 263 |
| MsG0780040117.01.T01 | MTR_4g045853 | 45.172 | 290 | 132 | 6 | 6 | 276 | 7 | 288 | 2.39e-87 | 263 |
| MsG0780040117.01.T01 | MTR_3g084620 | 45.890 | 292 | 135 | 4 | 1 | 280 | 1 | 281 | 1.56e-86 | 260 |
| MsG0780040117.01.T01 | MTR_3g084630 | 49.042 | 261 | 114 | 3 | 32 | 280 | 28 | 281 | 2.48e-85 | 257 |
| MsG0780040117.01.T01 | MTR_8g081040 | 49.805 | 257 | 111 | 4 | 32 | 276 | 31 | 281 | 3.85e-84 | 254 |
| MsG0780040117.01.T01 | MTR_7g093530 | 45.704 | 291 | 131 | 5 | 1 | 276 | 1 | 279 | 1.79e-83 | 252 |
| MsG0780040117.01.T01 | MTR_4g128580 | 48.485 | 264 | 113 | 4 | 32 | 276 | 26 | 285 | 7.12e-82 | 248 |
| MsG0780040117.01.T01 | MTR_1g105495 | 45.329 | 289 | 143 | 3 | 3 | 276 | 6 | 294 | 8.27e-82 | 248 |
| MsG0780040117.01.T01 | MTR_4g128570 | 49.805 | 257 | 102 | 5 | 32 | 276 | 31 | 272 | 1.02e-80 | 245 |
| MsG0780040117.01.T01 | MTR_1g105900 | 47.287 | 258 | 123 | 3 | 32 | 276 | 34 | 291 | 1.55e-80 | 245 |
| MsG0780040117.01.T01 | MTR_4g126920 | 47.126 | 261 | 118 | 4 | 32 | 276 | 25 | 281 | 5.24e-80 | 243 |
| MsG0780040117.01.T01 | MTR_6g088320 | 43.561 | 264 | 132 | 5 | 28 | 276 | 22 | 283 | 2.61e-79 | 242 |
| MsG0780040117.01.T01 | MTR_4g128400 | 49.200 | 250 | 114 | 4 | 39 | 276 | 35 | 283 | 1.80e-78 | 239 |
| MsG0780040117.01.T01 | MTR_8g102220 | 46.360 | 261 | 109 | 4 | 32 | 280 | 29 | 270 | 1.86e-77 | 236 |
| MsG0780040117.01.T01 | MTR_8g102910 | 42.500 | 280 | 144 | 5 | 12 | 276 | 2 | 279 | 2.80e-77 | 236 |
| MsG0780040117.01.T01 | MTR_2g038860 | 46.415 | 265 | 118 | 4 | 32 | 276 | 26 | 286 | 2.83e-77 | 237 |
| MsG0780040117.01.T01 | MTR_2g038705 | 46.415 | 265 | 118 | 4 | 32 | 276 | 26 | 286 | 2.83e-77 | 237 |
| MsG0780040117.01.T01 | MTR_8g013055 | 44.106 | 263 | 133 | 3 | 26 | 276 | 25 | 285 | 5.24e-77 | 236 |
| MsG0780040117.01.T01 | MTR_2g089140 | 49.333 | 225 | 95 | 4 | 73 | 278 | 1 | 225 | 6.72e-75 | 228 |
| MsG0780040117.01.T01 | MTR_4g128590 | 45.565 | 248 | 119 | 4 | 39 | 278 | 38 | 277 | 1.25e-70 | 219 |
| MsG0780040117.01.T01 | MTR_7g056090 | 41.696 | 283 | 137 | 7 | 7 | 277 | 16 | 282 | 1.16e-69 | 217 |
| MsG0780040117.01.T01 | MTR_3g089540 | 43.775 | 249 | 116 | 4 | 50 | 282 | 48 | 288 | 1.23e-69 | 217 |
| MsG0780040117.01.T01 | MTR_5g091730 | 45.000 | 240 | 114 | 5 | 51 | 278 | 47 | 280 | 2.53e-67 | 211 |
| MsG0780040117.01.T01 | MTR_2g049030 | 39.785 | 279 | 150 | 8 | 9 | 277 | 7 | 277 | 1.41e-61 | 196 |
| MsG0780040117.01.T01 | MTR_2g049130 | 41.036 | 251 | 130 | 7 | 29 | 266 | 26 | 271 | 6.06e-58 | 187 |
| MsG0780040117.01.T01 | MTR_5g029100 | 36.431 | 269 | 146 | 7 | 30 | 276 | 40 | 305 | 4.10e-56 | 184 |
| MsG0780040117.01.T01 | MTR_8g064180 | 35.547 | 256 | 143 | 6 | 43 | 277 | 57 | 311 | 3.50e-54 | 178 |
| MsG0780040117.01.T01 | MTR_8g090100 | 34.701 | 268 | 149 | 4 | 30 | 276 | 19 | 281 | 2.56e-53 | 176 |
| MsG0780040117.01.T01 | MTR_1g033420 | 34.932 | 292 | 163 | 8 | 10 | 279 | 8 | 294 | 1.61e-52 | 174 |
| MsG0780040117.01.T01 | MTR_2g095800 | 34.828 | 290 | 155 | 11 | 12 | 276 | 29 | 309 | 5.81e-49 | 164 |
| MsG0780040117.01.T01 | MTR_4g057450 | 32.660 | 297 | 161 | 9 | 10 | 276 | 17 | 304 | 1.75e-48 | 163 |
| MsG0780040117.01.T01 | MTR_5g029100 | 36.864 | 236 | 124 | 7 | 63 | 276 | 42 | 274 | 5.34e-48 | 162 |
| MsG0780040117.01.T01 | MTR_7g102260 | 35.606 | 264 | 142 | 9 | 32 | 276 | 37 | 291 | 2.24e-47 | 160 |
| MsG0780040117.01.T01 | MTR_1g063020 | 35.316 | 269 | 142 | 9 | 29 | 276 | 37 | 294 | 2.03e-46 | 157 |
| MsG0780040117.01.T01 | MTR_8g479340 | 33.333 | 186 | 63 | 3 | 92 | 276 | 10 | 135 | 8.11e-26 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040117.01.T01 | AT5G13870 | 72.696 | 293 | 69 | 1 | 1 | 282 | 1 | 293 | 2.73e-164 | 457 |
| MsG0780040117.01.T01 | AT5G13870 | 72.696 | 293 | 69 | 1 | 1 | 282 | 1 | 293 | 2.73e-164 | 457 |
| MsG0780040117.01.T01 | AT5G13870 | 72.696 | 293 | 69 | 1 | 1 | 282 | 1 | 293 | 2.73e-164 | 457 |
| MsG0780040117.01.T01 | AT2G06850 | 71.864 | 295 | 69 | 3 | 1 | 281 | 1 | 295 | 4.67e-160 | 447 |
| MsG0780040117.01.T01 | AT2G06850 | 76.623 | 231 | 43 | 2 | 62 | 281 | 5 | 235 | 1.05e-133 | 378 |
| MsG0780040117.01.T01 | AT5G65730 | 46.786 | 280 | 134 | 4 | 10 | 277 | 15 | 291 | 1.93e-92 | 275 |
| MsG0780040117.01.T01 | AT3G23730 | 48.611 | 288 | 123 | 6 | 10 | 280 | 11 | 290 | 2.02e-92 | 275 |
| MsG0780040117.01.T01 | AT4G37800 | 46.377 | 276 | 133 | 4 | 13 | 276 | 17 | 289 | 2.66e-88 | 265 |
| MsG0780040117.01.T01 | AT4G03210 | 48.485 | 264 | 121 | 3 | 32 | 282 | 29 | 290 | 4.34e-88 | 264 |
| MsG0780040117.01.T01 | AT4G14130 | 50.195 | 257 | 110 | 4 | 39 | 280 | 35 | 288 | 5.58e-88 | 264 |
| MsG0780040117.01.T01 | AT5G57560 | 46.207 | 290 | 126 | 6 | 5 | 276 | 4 | 281 | 3.49e-85 | 256 |
| MsG0780040117.01.T01 | AT5G57550 | 46.575 | 292 | 134 | 5 | 1 | 278 | 1 | 284 | 1.21e-84 | 255 |
| MsG0780040117.01.T01 | AT4G13090 | 46.263 | 281 | 133 | 5 | 11 | 277 | 13 | 289 | 2.14e-83 | 252 |
| MsG0780040117.01.T01 | AT1G11545 | 47.312 | 279 | 126 | 5 | 15 | 276 | 25 | 299 | 5.62e-83 | 252 |
| MsG0780040117.01.T01 | AT5G57530 | 50.391 | 256 | 108 | 6 | 39 | 279 | 34 | 285 | 4.02e-82 | 249 |
| MsG0780040117.01.T01 | AT4G30270 | 47.636 | 275 | 112 | 6 | 14 | 276 | 11 | 265 | 2.61e-81 | 246 |
| MsG0780040117.01.T01 | AT4G25810 | 46.454 | 282 | 118 | 7 | 12 | 276 | 18 | 283 | 7.57e-81 | 246 |
| MsG0780040117.01.T01 | AT5G57540 | 48.289 | 263 | 117 | 5 | 32 | 279 | 26 | 284 | 1.07e-80 | 245 |
| MsG0780040117.01.T01 | AT4G03210 | 46.094 | 256 | 122 | 3 | 40 | 282 | 10 | 262 | 1.68e-79 | 241 |
| MsG0780040117.01.T01 | AT3G25050 | 43.793 | 290 | 140 | 8 | 3 | 276 | 7 | 289 | 2.81e-79 | 242 |
| MsG0780040117.01.T01 | AT4G28850 | 41.608 | 286 | 149 | 5 | 11 | 280 | 8 | 291 | 5.45e-79 | 241 |
| MsG0780040117.01.T01 | AT5G48070 | 45.926 | 270 | 128 | 5 | 20 | 276 | 17 | 281 | 1.39e-78 | 240 |
| MsG0780040117.01.T01 | AT2G14620 | 44.291 | 289 | 140 | 6 | 1 | 276 | 14 | 294 | 7.24e-78 | 238 |
| MsG0780040117.01.T01 | AT4G13080 | 41.463 | 287 | 152 | 5 | 2 | 277 | 11 | 292 | 2.00e-76 | 234 |
| MsG0780040117.01.T01 | AT2G18800 | 41.751 | 297 | 141 | 7 | 9 | 276 | 3 | 296 | 1.15e-75 | 233 |
| MsG0780040117.01.T01 | AT4G25820 | 46.850 | 254 | 116 | 4 | 39 | 277 | 37 | 286 | 1.25e-74 | 230 |
| MsG0780040117.01.T01 | AT4G30290 | 47.917 | 240 | 108 | 4 | 49 | 276 | 42 | 276 | 3.52e-72 | 223 |
| MsG0780040117.01.T01 | AT2G14620 | 48.899 | 227 | 103 | 4 | 63 | 276 | 18 | 244 | 3.21e-71 | 220 |
| MsG0780040117.01.T01 | AT1G65310 | 49.115 | 226 | 98 | 4 | 63 | 276 | 61 | 281 | 3.80e-71 | 221 |
| MsG0780040117.01.T01 | AT1G65310 | 49.115 | 226 | 98 | 4 | 63 | 276 | 83 | 303 | 4.91e-71 | 221 |
| MsG0780040117.01.T01 | AT4G30280 | 48.230 | 226 | 100 | 4 | 63 | 276 | 61 | 281 | 8.77e-71 | 220 |
| MsG0780040117.01.T01 | AT2G14620 | 48.858 | 219 | 99 | 3 | 71 | 276 | 15 | 233 | 4.31e-69 | 214 |
| MsG0780040117.01.T01 | AT3G44990 | 37.086 | 302 | 155 | 8 | 1 | 276 | 1 | 293 | 2.80e-56 | 183 |
| MsG0780040117.01.T01 | AT1G32170 | 34.256 | 289 | 167 | 6 | 6 | 276 | 10 | 293 | 1.01e-53 | 178 |
| MsG0780040117.01.T01 | AT1G14720 | 31.507 | 292 | 182 | 5 | 1 | 276 | 1 | 290 | 3.01e-50 | 169 |
| MsG0780040117.01.T01 | AT1G10550 | 34.661 | 251 | 138 | 6 | 51 | 276 | 60 | 309 | 1.99e-49 | 166 |
| MsG0780040117.01.T01 | AT2G01850 | 32.632 | 285 | 172 | 6 | 10 | 276 | 8 | 290 | 7.86e-48 | 162 |
| MsG0780040117.01.T01 | AT3G48580 | 36.047 | 258 | 138 | 6 | 30 | 276 | 32 | 273 | 8.77e-48 | 160 |
| MsG0780040117.01.T01 | AT4G18990 | 34.524 | 252 | 135 | 5 | 54 | 276 | 62 | 312 | 2.91e-47 | 162 |
| MsG0780040117.01.T01 | AT2G36870 | 36.992 | 246 | 129 | 6 | 52 | 278 | 61 | 299 | 1.87e-46 | 158 |
| MsG0780040117.01.T01 | AT3G48580 | 35.841 | 226 | 119 | 5 | 62 | 276 | 4 | 214 | 4.35e-41 | 141 |
| MsG0780040117.01.T01 | AT4G18990 | 34.000 | 200 | 116 | 3 | 92 | 276 | 40 | 238 | 2.07e-40 | 142 |
| MsG0780040117.01.T01 | AT3G48580 | 30.620 | 258 | 132 | 7 | 30 | 276 | 32 | 253 | 2.18e-32 | 120 |
| MsG0780040117.01.T01 | AT2G36870 | 38.983 | 118 | 53 | 3 | 52 | 150 | 61 | 178 | 1.09e-19 | 84.7 |
Find 67 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGGTATACAACAGTTTA+TGG | 0.206946 | 7:+73229306 | MsG0780040117.01.T01:CDS |
| ACATTTGTTTGTAAAATATA+AGG | 0.260741 | 7:-73228514 | None:intergenic |
| TTCTTTGTTCTCTATTACCT+TGG | 0.292434 | 7:-73228549 | None:intergenic |
| ATTTCATCATGTTCCGCATT+TGG | 0.299391 | 7:-73228457 | None:intergenic |
| ATCAGTACAGTAATTATAAA+TGG | 0.326064 | 7:-73229549 | None:intergenic |
| GTACATCTACCGGCCTTTGC+GGG | 0.345454 | 7:-73227925 | None:intergenic |
| GTGCAAAACAAGGTAGAAAA+TGG | 0.352762 | 7:+73229453 | MsG0780040117.01.T01:CDS |
| ACCATAGATACTCAGTTCTA+TGG | 0.355438 | 7:+73228602 | MsG0780040117.01.T01:CDS |
| TTGTGGACAATATTCCAATC+AGG | 0.363427 | 7:+73229231 | MsG0780040117.01.T01:CDS |
| TACAAAAGTTTCCACATTGA+TGG | 0.366093 | 7:+73229401 | MsG0780040117.01.T01:CDS |
| AAACTGTTGTATACCTTCAT+TGG | 0.366710 | 7:-73229302 | None:intergenic |
| ACCTTCATTGGTTGATCAAA+TGG | 0.372148 | 7:-73229290 | None:intergenic |
| TCTAAAGGATCATACTTGTT+TGG | 0.386128 | 7:+73228197 | MsG0780040117.01.T01:CDS |
| CTCAATGGAAAAGGATAAAG+TGG | 0.398385 | 7:+73229510 | MsG0780040117.01.T01:CDS |
| TTTCATCATGTTCCGCATTT+GGG | 0.400987 | 7:-73228456 | None:intergenic |
| GTTTATGGAATGCAGATGAT+TGG | 0.401396 | 7:+73229321 | MsG0780040117.01.T01:CDS |
| ACAAGTATGATCCTTTAGAT+TGG | 0.403137 | 7:-73228193 | None:intergenic |
| TCCATAGAACTGAGTATCTA+TGG | 0.413646 | 7:-73228603 | None:intergenic |
| AGGCCGGTAGATGTACCTTT+TGG | 0.417023 | 7:+73227932 | MsG0780040117.01.T01:CDS |
| TCCATTTGATCAACCAATGA+AGG | 0.419877 | 7:+73229289 | MsG0780040117.01.T01:CDS |
| GAGAACAAAGAATATTTCTC+TGG | 0.421708 | 7:+73228560 | MsG0780040117.01.T01:CDS |
| TCACGGTCTCTTCTACATTC+AGG | 0.438875 | 7:-73229596 | None:intergenic |
| GGTACATCTACCGGCCTTTG+CGG | 0.443496 | 7:-73227926 | None:intergenic |
| CACATCAAATACTTTAATGA+AGG | 0.454409 | 7:+73227986 | MsG0780040117.01.T01:CDS |
| ATGAACATAAAGATGGTTCC+TGG | 0.454798 | 7:+73228230 | MsG0780040117.01.T01:CDS |
| TTACAAACAAATGTGTTCAC+TGG | 0.460974 | 7:+73228523 | MsG0780040117.01.T01:CDS |
| GTGACCTTGATGCTGCTCAA+TGG | 0.469367 | 7:+73229495 | MsG0780040117.01.T01:CDS |
| TTTATGGAATGCAGATGATT+GGG | 0.473254 | 7:+73229322 | MsG0780040117.01.T01:CDS |
| TTGATGCTGCTCAATGGAAA+AGG | 0.491805 | 7:+73229501 | MsG0780040117.01.T01:CDS |
| CAGCGCCACCCCGCAAAGGC+CGG | 0.496418 | 7:+73227916 | MsG0780040117.01.T01:CDS |
| GGTACTGGCTTCCAATCTAA+AGG | 0.501003 | 7:+73228182 | MsG0780040117.01.T01:intron |
| ATGTATGTGTAGGTTCTTTG+TGG | 0.502520 | 7:+73229214 | MsG0780040117.01.T01:intron |
| GGTACATAGTTTCGACCAAA+AGG | 0.510742 | 7:-73227947 | None:intergenic |
| CTTTCAGCGCCACCCCGCAA+AGG | 0.527079 | 7:+73227912 | MsG0780040117.01.T01:CDS |
| GTCGAAACTATGTACCCACT+TGG | 0.530233 | 7:+73227954 | MsG0780040117.01.T01:CDS |
| TCTTTATGTTCATGCTGAAG+TGG | 0.533369 | 7:-73228220 | None:intergenic |
| CTACATTCAGGAGGTATCTG+AGG | 0.537443 | 7:-73229584 | None:intergenic |
| CGACCAAAAGGTACATCTAC+CGG | 0.539669 | 7:-73227935 | None:intergenic |
| TGCAGATGATTGGGCAACAA+GGG | 0.548054 | 7:+73229331 | MsG0780040117.01.T01:CDS |
| ATGCAGATGATTGGGCAACA+AGG | 0.548692 | 7:+73229330 | MsG0780040117.01.T01:CDS |
| AATGTGTTCACTGGAGGCCA+AGG | 0.551160 | 7:+73228532 | MsG0780040117.01.T01:CDS |
| TGTGGACAATATTCCAATCA+GGG | 0.551213 | 7:+73229232 | MsG0780040117.01.T01:CDS |
| TCAAAAGCACCATTCATAGC+AGG | 0.555389 | 7:+73229377 | MsG0780040117.01.T01:CDS |
| CTTCAGCATGAACATAAAGA+TGG | 0.562772 | 7:+73228223 | MsG0780040117.01.T01:CDS |
| GTGGATTAGAGAAAACTGAT+TGG | 0.570924 | 7:+73229354 | MsG0780040117.01.T01:CDS |
| CGGGGTGGCGCTGAAAGCCG+CGG | 0.571142 | 7:-73227906 | None:intergenic |
| GATGATTGGGCAACAAGGGG+TGG | 0.574149 | 7:+73229335 | MsG0780040117.01.T01:CDS |
| TACATCTACCGGCCTTTGCG+GGG | 0.574701 | 7:-73227924 | None:intergenic |
| GCATCAAGGTCACGAAACTC+AGG | 0.577476 | 7:-73229485 | None:intergenic |
| CTTCTTCTTGATAAGTACAC+TGG | 0.587312 | 7:+73228022 | MsG0780040117.01.T01:CDS |
| TGCAGTTATCTTCCCAAATG+CGG | 0.596096 | 7:+73228444 | MsG0780040117.01.T01:intron |
| ATCTACCGGCCTTTGCGGGG+TGG | 0.599027 | 7:-73227921 | None:intergenic |
| TAGAACTGAGTATCTATGGT+AGG | 0.601747 | 7:-73228599 | None:intergenic |
| GCAAAGTACTGTGCAAAACA+AGG | 0.603844 | 7:+73229443 | MsG0780040117.01.T01:CDS |
| TACATTCAGGAGGTATCTGA+GGG | 0.605395 | 7:-73229583 | None:intergenic |
| TCGAAACTATGTACCCACTT+GGG | 0.605536 | 7:+73227955 | MsG0780040117.01.T01:CDS |
| CAAAACAAGGTAGAAAATGG+TGG | 0.627129 | 7:+73229456 | MsG0780040117.01.T01:CDS |
| CAAACAAATGTGTTCACTGG+AGG | 0.629079 | 7:+73228526 | MsG0780040117.01.T01:CDS |
| TTTGTTGTGTCTAACGTCCG+CGG | 0.640803 | 7:+73227889 | MsG0780040117.01.T01:CDS |
| CAAGGTCACGAAACTCAGGT+TGG | 0.649125 | 7:-73229481 | None:intergenic |
| GCAGATGATTGGGCAACAAG+GGG | 0.654510 | 7:+73229332 | MsG0780040117.01.T01:CDS |
| ACAGTACTTTGCATTAACTG+AGG | 0.671350 | 7:-73229432 | None:intergenic |
| CGGTCTCTTCTACATTCAGG+AGG | 0.676223 | 7:-73229593 | None:intergenic |
| ATGTGATCAAAAGCCCAAGT+GGG | 0.680372 | 7:-73227968 | None:intergenic |
| TCAATGGAAAAGGATAAAGT+GGG | 0.694713 | 7:+73229511 | MsG0780040117.01.T01:CDS |
| GATGTGATCAAAAGCCCAAG+TGG | 0.698619 | 7:-73227969 | None:intergenic |
| AGGTTTCACAACCATCAATG+TGG | 0.700769 | 7:-73229412 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTAATATATGTCTTAT+TGG | + | Chr7:73228094-73228113 | MsG0780040117.01.T01:intron | 10.0% |
| !!! | TATTTATACAATTTTTGAAT+AGG | + | Chr7:73228161-73228180 | MsG0780040117.01.T01:intron | 10.0% |
| !! | ACATTTGTTTGTAAAATATA+AGG | - | Chr7:73228517-73228536 | None:intergenic | 15.0% |
| !! | ATCCCTAAATAAATAAATTT+CGG | - | Chr7:73228903-73228922 | None:intergenic | 15.0% |
| !! | GTTAATTAAGATTCTATATA+CGG | - | Chr7:73228842-73228861 | None:intergenic | 15.0% |
| !! | TTAATTAAGATTCTATATAC+GGG | - | Chr7:73228841-73228860 | None:intergenic | 15.0% |
| !! | TTCTACATAGAAATTAATTA+AGG | - | Chr7:73228064-73228083 | None:intergenic | 15.0% |
| !! | AAATTAATCATCTTTCATGA+AGG | - | Chr7:73229045-73229064 | None:intergenic | 20.0% |
| !! | AATTAATCATCTTTCATGAA+GGG | - | Chr7:73229044-73229063 | None:intergenic | 20.0% |
| !! | AGACCGAAATTTATTTATTT+AGG | + | Chr7:73228897-73228916 | MsG0780040117.01.T01:intron | 20.0% |
| !! | ATCAAAACGTCATATAAATT+AGG | - | Chr7:73229122-73229141 | None:intergenic | 20.0% |
| !! | ATCAGTACAGTAATTATAAA+TGG | - | Chr7:73229552-73229571 | None:intergenic | 20.0% |
| !! | GACCGAAATTTATTTATTTA+GGG | + | Chr7:73228898-73228917 | MsG0780040117.01.T01:intron | 20.0% |
| !!! | CTATCATAGTAATTTTTTAC+TGG | + | Chr7:73229074-73229093 | MsG0780040117.01.T01:intron | 20.0% |
| !!! | GAAATAGATTTTGAGTTTTT+GGG | + | Chr7:73228475-73228494 | MsG0780040117.01.T01:CDS | 20.0% |
| !!! | TGAAATAGATTTTGAGTTTT+TGG | + | Chr7:73228474-73228493 | MsG0780040117.01.T01:CDS | 20.0% |
| ! | AAACGTCATATAAATTAGGA+CGG | - | Chr7:73229118-73229137 | None:intergenic | 25.0% |
| ! | AAATTCACATATGTATGTGT+AGG | + | Chr7:73229204-73229223 | MsG0780040117.01.T01:intron | 25.0% |
| ! | CACATCAAATACTTTAATGA+AGG | + | Chr7:73227986-73228005 | MsG0780040117.01.T01:CDS | 25.0% |
| ! | TAGATAGAAAGATACTAACA+CGG | + | Chr7:73228731-73228750 | MsG0780040117.01.T01:intron | 25.0% |
| !! | AATTGATCCAATTTTGTAGT+GGG | + | Chr7:73228348-73228367 | MsG0780040117.01.T01:intron | 25.0% |
| !! | TACAATTTTTGAATAGGTAC+TGG | + | Chr7:73228167-73228186 | MsG0780040117.01.T01:intron | 25.0% |
| !!! | GTTTTGAAGTCTTTCTTAAT+AGG | + | Chr7:73228772-73228791 | MsG0780040117.01.T01:intron | 25.0% |
| AAACTGTTGTATACCTTCAT+TGG | - | Chr7:73229305-73229324 | None:intergenic | 30.0% | |
| AATTAACCCCACTACAAAAT+TGG | - | Chr7:73228358-73228377 | None:intergenic | 30.0% | |
| ACAAGTATGATCCTTTAGAT+TGG | - | Chr7:73228196-73228215 | None:intergenic | 30.0% | |
| CCAAATCAGACAAAACTTAT+GGG | - | Chr7:73228656-73228675 | None:intergenic | 30.0% | |
| GAGAACAAAGAATATTTCTC+TGG | + | Chr7:73228560-73228579 | MsG0780040117.01.T01:CDS | 30.0% | |
| TACAAAAGTTTCCACATTGA+TGG | + | Chr7:73229401-73229420 | MsG0780040117.01.T01:CDS | 30.0% | |
| TCAATGGAAAAGGATAAAGT+GGG | + | Chr7:73229511-73229530 | MsG0780040117.01.T01:CDS | 30.0% | |
| TCTAAAGGATCATACTTGTT+TGG | + | Chr7:73228197-73228216 | MsG0780040117.01.T01:CDS | 30.0% | |
| TTACAAACAAATGTGTTCAC+TGG | + | Chr7:73228523-73228542 | MsG0780040117.01.T01:CDS | 30.0% | |
| TTCAAAAACAGCAAGAAGTT+AGG | + | Chr7:73229257-73229276 | MsG0780040117.01.T01:CDS | 30.0% | |
| TTCTTTGTTCTCTATTACCT+TGG | - | Chr7:73228552-73228571 | None:intergenic | 30.0% | |
| TTTATGGAATGCAGATGATT+GGG | + | Chr7:73229322-73229341 | MsG0780040117.01.T01:CDS | 30.0% | |
| ! | ATTGATCCAATTTTGTAGTG+GGG | + | Chr7:73228349-73228368 | MsG0780040117.01.T01:intron | 30.0% |
| ! | GAATTGATCCAATTTTGTAG+TGG | + | Chr7:73228347-73228366 | MsG0780040117.01.T01:intron | 30.0% |
| ! | TGAAGGTATACAACAGTTTA+TGG | + | Chr7:73229306-73229325 | MsG0780040117.01.T01:CDS | 30.0% |
| ! | TTTTGTGCATACATAGAAAG+CGG | - | Chr7:73228276-73228295 | None:intergenic | 30.0% |
| !! | CCATAAGTTTTGTCTGATTT+GGG | + | Chr7:73228654-73228673 | MsG0780040117.01.T01:intron | 30.0% |
| ACAGTACTTTGCATTAACTG+AGG | - | Chr7:73229435-73229454 | None:intergenic | 35.0% | |
| ACCATAGATACTCAGTTCTA+TGG | + | Chr7:73228602-73228621 | MsG0780040117.01.T01:CDS | 35.0% | |
| ATAGGATGACTAAAATCGCA+TGG | + | Chr7:73228790-73228809 | MsG0780040117.01.T01:intron | 35.0% | |
| ATGAACATAAAGATGGTTCC+TGG | + | Chr7:73228230-73228249 | MsG0780040117.01.T01:CDS | 35.0% | |
| ATGTATGTGTAGGTTCTTTG+TGG | + | Chr7:73229214-73229233 | MsG0780040117.01.T01:intron | 35.0% | |
| ATTTCATCATGTTCCGCATT+TGG | - | Chr7:73228460-73228479 | None:intergenic | 35.0% | |
| CAAAACAAGGTAGAAAATGG+TGG | + | Chr7:73229456-73229475 | MsG0780040117.01.T01:CDS | 35.0% | |
| CCCAAATCAGACAAAACTTA+TGG | - | Chr7:73228657-73228676 | None:intergenic | 35.0% | |
| CTCAATGGAAAAGGATAAAG+TGG | + | Chr7:73229510-73229529 | MsG0780040117.01.T01:CDS | 35.0% | |
| CTTCAGCATGAACATAAAGA+TGG | + | Chr7:73228223-73228242 | MsG0780040117.01.T01:CDS | 35.0% | |
| CTTCTTCTTGATAAGTACAC+TGG | + | Chr7:73228022-73228041 | MsG0780040117.01.T01:CDS | 35.0% | |
| GTGCAAAACAAGGTAGAAAA+TGG | + | Chr7:73229453-73229472 | MsG0780040117.01.T01:CDS | 35.0% | |
| GTTTATGGAATGCAGATGAT+TGG | + | Chr7:73229321-73229340 | MsG0780040117.01.T01:CDS | 35.0% | |
| TAGAACTGAGTATCTATGGT+AGG | - | Chr7:73228602-73228621 | None:intergenic | 35.0% | |
| TCCATAGAACTGAGTATCTA+TGG | - | Chr7:73228606-73228625 | None:intergenic | 35.0% | |
| TCCATTTGATCAACCAATGA+AGG | + | Chr7:73229289-73229308 | MsG0780040117.01.T01:CDS | 35.0% | |
| TCTTTATGTTCATGCTGAAG+TGG | - | Chr7:73228223-73228242 | None:intergenic | 35.0% | |
| TGTGGACAATATTCCAATCA+GGG | + | Chr7:73229232-73229251 | MsG0780040117.01.T01:CDS | 35.0% | |
| TTGTGGACAATATTCCAATC+AGG | + | Chr7:73229231-73229250 | MsG0780040117.01.T01:CDS | 35.0% | |
| TTTCATCATGTTCCGCATTT+GGG | - | Chr7:73228459-73228478 | None:intergenic | 35.0% | |
| ! | ACCTTCATTGGTTGATCAAA+TGG | - | Chr7:73229293-73229312 | None:intergenic | 35.0% |
| ! | CTTTTGTAACCTGCTATGAA+TGG | - | Chr7:73229389-73229408 | None:intergenic | 35.0% |
| ! | TATCTATGGTAGGCTTTTGT+AGG | - | Chr7:73228592-73228611 | None:intergenic | 35.0% |
| !! | CCCATAAGTTTTGTCTGATT+TGG | + | Chr7:73228653-73228672 | MsG0780040117.01.T01:intron | 35.0% |
| !! | GTGGATTAGAGAAAACTGAT+TGG | + | Chr7:73229354-73229373 | MsG0780040117.01.T01:CDS | 35.0% |
| AGGTTTCACAACCATCAATG+TGG | - | Chr7:73229415-73229434 | None:intergenic | 40.0% | |
| ATGTGATCAAAAGCCCAAGT+GGG | - | Chr7:73227971-73227990 | None:intergenic | 40.0% | |
| CAAAACTCCAAGTCAAAGCA+TGG | - | Chr7:73228758-73228777 | None:intergenic | 40.0% | |
| CAAACAAATGTGTTCACTGG+AGG | + | Chr7:73228526-73228545 | MsG0780040117.01.T01:CDS | 40.0% | |
| GGTACATAGTTTCGACCAAA+AGG | - | Chr7:73227950-73227969 | None:intergenic | 40.0% | |
| TCGAAACTATGTACCCACTT+GGG | + | Chr7:73227955-73227974 | MsG0780040117.01.T01:CDS | 40.0% | |
| TGCAGTTATCTTCCCAAATG+CGG | + | Chr7:73228444-73228463 | MsG0780040117.01.T01:intron | 40.0% | |
| TTGATGCTGCTCAATGGAAA+AGG | + | Chr7:73229501-73229520 | MsG0780040117.01.T01:CDS | 40.0% | |
| ! | GATAAGTTGAAACGTGAGTC+AGG | + | Chr7:73228940-73228959 | MsG0780040117.01.T01:intron | 40.0% |
| ! | TTTTCCATTGAGCAGCATCA+AGG | - | Chr7:73229502-73229521 | None:intergenic | 40.0% |
| !! | GCAAAGTACTGTGCAAAACA+AGG | + | Chr7:73229443-73229462 | MsG0780040117.01.T01:CDS | 40.0% |
| !! | TACATTCAGGAGGTATCTGA+GGG | - | Chr7:73229586-73229605 | None:intergenic | 40.0% |
| !! | TCAAAAGCACCATTCATAGC+AGG | + | Chr7:73229377-73229396 | MsG0780040117.01.T01:CDS | 40.0% |
| !!! | CTGTTTTTGAACACCCTGAT+TGG | - | Chr7:73229248-73229267 | None:intergenic | 40.0% |
| !!! | GAGTTTTTGGGTAACAGAAC+AGG | + | Chr7:73228487-73228506 | MsG0780040117.01.T01:CDS | 40.0% |
| ATGCAGATGATTGGGCAACA+AGG | + | Chr7:73229330-73229349 | MsG0780040117.01.T01:CDS | 45.0% | |
| GATGTGATCAAAAGCCCAAG+TGG | - | Chr7:73227972-73227991 | None:intergenic | 45.0% | |
| GTCGAAACTATGTACCCACT+TGG | + | Chr7:73227954-73227973 | MsG0780040117.01.T01:CDS | 45.0% | |
| TCACGGTCTCTTCTACATTC+AGG | - | Chr7:73229599-73229618 | None:intergenic | 45.0% | |
| TGCAGATGATTGGGCAACAA+GGG | + | Chr7:73229331-73229350 | MsG0780040117.01.T01:CDS | 45.0% | |
| ! | CGACCAAAAGGTACATCTAC+CGG | - | Chr7:73227938-73227957 | None:intergenic | 45.0% |
| ! | TTTGTTGTGTCTAACGTCCG+CGG | + | Chr7:73227889-73227908 | MsG0780040117.01.T01:CDS | 45.0% |
| !! | CTACATTCAGGAGGTATCTG+AGG | - | Chr7:73229587-73229606 | None:intergenic | 45.0% |
| !! | GGACGGAGAAAGTACTATGA+TGG | - | Chr7:73229101-73229120 | None:intergenic | 45.0% |
| !! | GGTACTGGCTTCCAATCTAA+AGG | + | Chr7:73228182-73228201 | MsG0780040117.01.T01:intron | 45.0% |
| AATGTGTTCACTGGAGGCCA+AGG | + | Chr7:73228532-73228551 | MsG0780040117.01.T01:CDS | 50.0% | |
| ACACGGTCCATGCTTTGACT+TGG | + | Chr7:73228748-73228767 | MsG0780040117.01.T01:intron | 50.0% | |
| ACTGTTCCAGCTGAATCACC+AGG | - | Chr7:73228251-73228270 | None:intergenic | 50.0% | |
| ATGGTTCCTGGTGATTCAGC+TGG | + | Chr7:73228242-73228261 | MsG0780040117.01.T01:intron | 50.0% | |
| CAAGGTCACGAAACTCAGGT+TGG | - | Chr7:73229484-73229503 | None:intergenic | 50.0% | |
| CGGTCTCTTCTACATTCAGG+AGG | - | Chr7:73229596-73229615 | None:intergenic | 50.0% | |
| GCAGATGATTGGGCAACAAG+GGG | + | Chr7:73229332-73229351 | MsG0780040117.01.T01:CDS | 50.0% | |
| GCATCAAGGTCACGAAACTC+AGG | - | Chr7:73229488-73229507 | None:intergenic | 50.0% | |
| GGGATATCACGTGCACAACA+TGG | + | Chr7:73228918-73228937 | MsG0780040117.01.T01:intron | 50.0% | |
| GTGACCTTGATGCTGCTCAA+TGG | + | Chr7:73229495-73229514 | MsG0780040117.01.T01:CDS | 50.0% | |
| ! | AGGCCGGTAGATGTACCTTT+TGG | + | Chr7:73227932-73227951 | MsG0780040117.01.T01:CDS | 50.0% |
| ! | TGGGGTTTTCGTCGTGGATT+TGG | + | Chr7:73227858-73227877 | MsG0780040117.01.T01:CDS | 50.0% |
| GATGATTGGGCAACAAGGGG+TGG | + | Chr7:73229335-73229354 | MsG0780040117.01.T01:CDS | 55.0% | |
| GGTACATCTACCGGCCTTTG+CGG | - | Chr7:73227929-73227948 | None:intergenic | 55.0% | |
| GTACATCTACCGGCCTTTGC+GGG | - | Chr7:73227928-73227947 | None:intergenic | 55.0% | |
| TACATCTACCGGCCTTTGCG+GGG | - | Chr7:73227927-73227946 | None:intergenic | 55.0% | |
| ATCTACCGGCCTTTGCGGGG+TGG | - | Chr7:73227924-73227943 | None:intergenic | 65.0% | |
| CTTTCAGCGCCACCCCGCAA+AGG | + | Chr7:73227912-73227931 | MsG0780040117.01.T01:CDS | 65.0% | |
| CAGCGCCACCCCGCAAAGGC+CGG | + | Chr7:73227916-73227935 | MsG0780040117.01.T01:CDS | 75.0% | |
| ! | CGGGGTGGCGCTGAAAGCCG+CGG | - | Chr7:73227909-73227928 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 73227857 | 73229625 | 73227857 | ID=MsG0780040117.01;Name=MsG0780040117.01 |
| Chr7 | mRNA | 73227857 | 73229625 | 73227857 | ID=MsG0780040117.01.T01;Parent=MsG0780040117.01;Name=MsG0780040117.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|4|0|282 |
| Chr7 | exon | 73227857 | 73228043 | 73227857 | ID=MsG0780040117.01.T01:exon:13185;Parent=MsG0780040117.01.T01 |
| Chr7 | exon | 73228183 | 73228251 | 73228183 | ID=MsG0780040117.01.T01:exon:13186;Parent=MsG0780040117.01.T01 |
| Chr7 | exon | 73228449 | 73228641 | 73228449 | ID=MsG0780040117.01.T01:exon:13187;Parent=MsG0780040117.01.T01 |
| Chr7 | exon | 73229226 | 73229625 | 73229226 | ID=MsG0780040117.01.T01:exon:13188;Parent=MsG0780040117.01.T01 |
| Chr7 | CDS | 73227857 | 73228043 | 73227857 | ID=MsG0780040117.01.T01:cds;Parent=MsG0780040117.01.T01 |
| Chr7 | CDS | 73228183 | 73228251 | 73228183 | ID=MsG0780040117.01.T01:cds;Parent=MsG0780040117.01.T01 |
| Chr7 | CDS | 73228449 | 73228641 | 73228449 | ID=MsG0780040117.01.T01:cds;Parent=MsG0780040117.01.T01 |
| Chr7 | CDS | 73229226 | 73229625 | 73229226 | ID=MsG0780040117.01.T01:cds;Parent=MsG0780040117.01.T01 |
| Gene Sequence |
| Protein sequence |
