Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040240.01.T01 | MCI28199.1 | 91.935 | 62 | 5 | 0 | 1 | 62 | 42 | 103 | 4.08E-31 | 116 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040240.01.T01 | Q9SH76 | 91.935 | 62 | 5 | 0 | 1 | 62 | 265 | 326 | 5.79E-32 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040240.01.T01 | A0A2I0W9D0 | 93.548 | 62 | 4 | 0 | 1 | 62 | 264 | 325 | 5.28e-30 | 121 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000470.01 | MsG0780040240.01 | 0.826117 | 3.234499e-54 | 3.005758e-51 |
| MsG0180001884.01 | MsG0780040240.01 | 0.802175 | 6.356096e-49 | 3.092695e-46 |
| MsG0180002842.01 | MsG0780040240.01 | 0.816172 | 6.343396e-52 | 4.462262e-49 |
| MsG0180004789.01 | MsG0780040240.01 | 0.828788 | 7.399241e-55 | 7.429486e-52 |
| MsG0280006299.01 | MsG0780040240.01 | 0.802903 | 4.498824e-49 | 2.230050e-46 |
| MsG0780040240.01 | MsG0780041456.01 | 0.800761 | 1.238127e-48 | 5.812441e-46 |
| MsG0480022259.01 | MsG0780040240.01 | 0.808242 | 3.410444e-50 | 1.940059e-47 |
| MsG0480022262.01 | MsG0780040240.01 | 0.804019 | 2.641223e-49 | 1.347267e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040240.01.T01 | MTR_2g104860 | 93.548 | 62 | 4 | 0 | 1 | 62 | 262 | 323 | 1.85e-33 | 120 |
| MsG0780040240.01.T01 | MTR_3g108800 | 91.935 | 62 | 5 | 0 | 1 | 62 | 264 | 325 | 6.72e-33 | 119 |
| MsG0780040240.01.T01 | MTR_1g009760 | 91.935 | 62 | 5 | 0 | 1 | 62 | 264 | 325 | 6.72e-33 | 119 |
| MsG0780040240.01.T01 | MTR_4g127710 | 91.935 | 62 | 5 | 0 | 1 | 62 | 267 | 328 | 7.40e-33 | 119 |
| MsG0780040240.01.T01 | MTR_3g108800 | 91.935 | 62 | 5 | 0 | 1 | 62 | 263 | 324 | 7.92e-33 | 119 |
| MsG0780040240.01.T01 | MTR_4g127710 | 91.935 | 62 | 5 | 0 | 1 | 62 | 267 | 328 | 8.40e-33 | 119 |
| MsG0780040240.01.T01 | MTR_1g009720 | 91.935 | 62 | 5 | 0 | 1 | 62 | 264 | 325 | 9.44e-33 | 119 |
| MsG0780040240.01.T01 | MTR_1g009760 | 91.935 | 62 | 5 | 0 | 1 | 62 | 267 | 328 | 2.76e-32 | 117 |
| MsG0780040240.01.T01 | MTR_4g116950 | 90.323 | 62 | 6 | 0 | 1 | 62 | 265 | 326 | 3.09e-32 | 117 |
| MsG0780040240.01.T01 | MTR_8g063880 | 90.323 | 62 | 6 | 0 | 1 | 62 | 264 | 325 | 3.86e-32 | 117 |
| MsG0780040240.01.T01 | MTR_4g107500 | 90.323 | 62 | 6 | 0 | 1 | 62 | 268 | 329 | 4.56e-32 | 117 |
| MsG0780040240.01.T01 | MTR_6g011310 | 90.323 | 62 | 6 | 0 | 1 | 62 | 266 | 327 | 4.60e-32 | 117 |
| MsG0780040240.01.T01 | MTR_6g011310 | 90.323 | 62 | 6 | 0 | 1 | 62 | 266 | 327 | 4.74e-32 | 117 |
| MsG0780040240.01.T01 | MTR_2g036650 | 88.710 | 62 | 7 | 0 | 1 | 62 | 269 | 330 | 6.04e-32 | 116 |
| MsG0780040240.01.T01 | MTR_2g449840 | 90.323 | 62 | 6 | 0 | 1 | 62 | 265 | 326 | 6.09e-32 | 116 |
| MsG0780040240.01.T01 | MTR_7g117500 | 82.258 | 62 | 11 | 0 | 1 | 62 | 271 | 332 | 2.16e-29 | 109 |
| MsG0780040240.01.T01 | MTR_7g117500 | 82.258 | 62 | 11 | 0 | 1 | 62 | 271 | 332 | 2.50e-29 | 109 |
| MsG0780040240.01.T01 | MTR_8g006790 | 80.645 | 62 | 12 | 0 | 1 | 62 | 272 | 333 | 5.04e-28 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040240.01.T01 | AT2G07560 | 91.935 | 62 | 5 | 0 | 1 | 62 | 265 | 326 | 5.91e-33 | 119 |
| MsG0780040240.01.T01 | AT2G18960 | 91.935 | 62 | 5 | 0 | 1 | 62 | 262 | 323 | 7.04e-33 | 119 |
| MsG0780040240.01.T01 | AT4G30190 | 91.935 | 62 | 5 | 0 | 1 | 62 | 262 | 323 | 8.88e-33 | 119 |
| MsG0780040240.01.T01 | AT2G18960 | 91.935 | 62 | 5 | 0 | 1 | 62 | 198 | 259 | 9.49e-33 | 119 |
| MsG0780040240.01.T01 | AT2G18960 | 91.935 | 62 | 5 | 0 | 1 | 62 | 198 | 259 | 9.49e-33 | 119 |
| MsG0780040240.01.T01 | AT4G30190 | 91.935 | 62 | 5 | 0 | 1 | 62 | 262 | 323 | 1.09e-32 | 119 |
| MsG0780040240.01.T01 | AT1G80660 | 90.323 | 62 | 6 | 0 | 1 | 62 | 267 | 328 | 3.46e-32 | 117 |
| MsG0780040240.01.T01 | AT1G80660 | 90.323 | 62 | 6 | 0 | 1 | 62 | 267 | 328 | 3.46e-32 | 117 |
| MsG0780040240.01.T01 | AT1G80660 | 90.323 | 62 | 6 | 0 | 1 | 62 | 267 | 328 | 3.46e-32 | 117 |
| MsG0780040240.01.T01 | AT3G42640 | 90.323 | 62 | 6 | 0 | 1 | 62 | 265 | 326 | 5.16e-32 | 117 |
| MsG0780040240.01.T01 | AT1G80660 | 90.323 | 62 | 6 | 0 | 1 | 62 | 138 | 199 | 7.20e-32 | 116 |
| MsG0780040240.01.T01 | AT3G47950 | 90.323 | 62 | 6 | 0 | 1 | 62 | 270 | 331 | 1.16e-31 | 115 |
| MsG0780040240.01.T01 | AT3G47950 | 90.323 | 62 | 6 | 0 | 1 | 62 | 270 | 331 | 1.16e-31 | 115 |
| MsG0780040240.01.T01 | AT5G57350 | 88.710 | 62 | 7 | 0 | 1 | 62 | 263 | 324 | 1.20e-31 | 115 |
| MsG0780040240.01.T01 | AT5G57350 | 88.710 | 62 | 7 | 0 | 1 | 62 | 263 | 324 | 1.20e-31 | 115 |
| MsG0780040240.01.T01 | AT5G57350 | 88.710 | 62 | 7 | 0 | 1 | 62 | 263 | 324 | 1.20e-31 | 115 |
| MsG0780040240.01.T01 | AT5G62670 | 90.323 | 62 | 6 | 0 | 1 | 62 | 266 | 327 | 1.29e-31 | 115 |
| MsG0780040240.01.T01 | AT5G57350 | 88.710 | 62 | 7 | 0 | 1 | 62 | 1 | 62 | 1.77e-31 | 115 |
| MsG0780040240.01.T01 | AT2G24520 | 85.484 | 62 | 9 | 0 | 1 | 62 | 1 | 62 | 2.65e-30 | 112 |
| MsG0780040240.01.T01 | AT2G24520 | 85.484 | 62 | 9 | 0 | 1 | 62 | 244 | 305 | 2.69e-30 | 112 |
| MsG0780040240.01.T01 | AT2G24520 | 85.484 | 62 | 9 | 0 | 1 | 62 | 244 | 305 | 2.69e-30 | 112 |
| MsG0780040240.01.T01 | AT2G24520 | 85.484 | 62 | 9 | 0 | 1 | 62 | 307 | 368 | 2.88e-30 | 112 |
| MsG0780040240.01.T01 | AT1G17260 | 83.871 | 62 | 10 | 0 | 1 | 62 | 270 | 331 | 1.69e-29 | 109 |
| MsG0780040240.01.T01 | AT3G60330 | 82.258 | 62 | 11 | 0 | 1 | 62 | 265 | 326 | 1.92e-28 | 106 |
| MsG0780040240.01.T01 | AT3G60330 | 82.258 | 62 | 11 | 0 | 1 | 62 | 265 | 326 | 1.92e-28 | 106 |
| MsG0780040240.01.T01 | AT3G60330 | 82.258 | 62 | 11 | 0 | 1 | 62 | 265 | 326 | 2.06e-28 | 106 |
| MsG0780040240.01.T01 | AT3G60330 | 82.258 | 62 | 11 | 0 | 1 | 62 | 265 | 326 | 2.06e-28 | 106 |
| MsG0780040240.01.T01 | AT4G11730 | 58.065 | 62 | 26 | 0 | 1 | 62 | 264 | 325 | 7.55e-17 | 73.6 |
Find 21 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATACTTGCGGCACTGAAT+AGG | 0.375229 | 7:-74793648 | None:intergenic |
| AATGATAGCCATTGAGGAAA+TGG | 0.389631 | 7:+74793797 | MsG0780040240.01.T01:CDS |
| TTGTCACTTACAATGGCTAT+TGG | 0.393257 | 7:+74793735 | MsG0780040240.01.T01:CDS |
| AAAACTGTTGGCATTGCAAT+AGG | 0.398141 | 7:-74793714 | None:intergenic |
| AATCTATTGGTGCTTCTGAT+TGG | 0.400461 | 7:+74793684 | MsG0780040240.01.T01:CDS |
| CTATTCAGTGCCGCAAGTAT+AGG | 0.401900 | 7:+74793649 | MsG0780040240.01.T01:CDS |
| CATTGTAGCCATTTCCTCAA+TGG | 0.448622 | 7:-74793805 | None:intergenic |
| GGCTACAATGGTGGCTGCAA+TGG | 0.465789 | 7:+74793818 | MsG0780040240.01.T01:CDS |
| CTATTGGTGCTTCTGATTGG+AGG | 0.482388 | 7:+74793687 | MsG0780040240.01.T01:CDS |
| AACAACAGTTATACTAAGGT+TGG | 0.501460 | 7:-74793876 | None:intergenic |
| CTCCAACAACAGTTATACTA+AGG | 0.502626 | 7:-74793880 | None:intergenic |
| GAGTGGAATTGACAATCTAT+TGG | 0.533730 | 7:+74793671 | MsG0780040240.01.T01:CDS |
| AAAGAGAATGATAGCCATTG+AGG | 0.536593 | 7:+74793791 | MsG0780040240.01.T01:CDS |
| TATACTAAGGTTGGATCATG+TGG | 0.537117 | 7:-74793867 | None:intergenic |
| CAATGGCTATTGGTTCACAT+AGG | 0.569105 | 7:+74793745 | MsG0780040240.01.T01:CDS |
| CAATTCCACTCCTATACTTG+CGG | 0.571367 | 7:-74793659 | None:intergenic |
| ATTGTAAGTGACAAAACTGT+TGG | 0.597604 | 7:-74793726 | None:intergenic |
| CATTGAGGAAATGGCTACAA+TGG | 0.598108 | 7:+74793806 | MsG0780040240.01.T01:CDS |
| CAGTGCCGCAAGTATAGGAG+TGG | 0.639208 | 7:+74793654 | MsG0780040240.01.T01:CDS |
| TCACATAGGTTGTCTCAGCA+AGG | 0.643329 | 7:+74793759 | MsG0780040240.01.T01:CDS |
| TGAGGAAATGGCTACAATGG+TGG | 0.680612 | 7:+74793809 | MsG0780040240.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGTAAGTGACAAAACTGT+TGG | - | Chr7:74793729-74793748 | None:intergenic | 30.0% | |
| !! | AACAGTTTTGTCACTTACAA+TGG | + | Chr7:74793728-74793747 | MsG0780040240.01.T01:CDS | 30.0% |
| AAAACTGTTGGCATTGCAAT+AGG | - | Chr7:74793717-74793736 | None:intergenic | 35.0% | |
| AAAGAGAATGATAGCCATTG+AGG | + | Chr7:74793791-74793810 | MsG0780040240.01.T01:CDS | 35.0% | |
| AATGATAGCCATTGAGGAAA+TGG | + | Chr7:74793797-74793816 | MsG0780040240.01.T01:CDS | 35.0% | |
| GAGTGGAATTGACAATCTAT+TGG | + | Chr7:74793671-74793690 | MsG0780040240.01.T01:CDS | 35.0% | |
| TATACTAAGGTTGGATCATG+TGG | - | Chr7:74793870-74793889 | None:intergenic | 35.0% | |
| ! | AATCTATTGGTGCTTCTGAT+TGG | + | Chr7:74793684-74793703 | MsG0780040240.01.T01:CDS | 35.0% |
| ! | TTGTCACTTACAATGGCTAT+TGG | + | Chr7:74793735-74793754 | MsG0780040240.01.T01:CDS | 35.0% |
| CAATTCCACTCCTATACTTG+CGG | - | Chr7:74793662-74793681 | None:intergenic | 40.0% | |
| CATTGAGGAAATGGCTACAA+TGG | + | Chr7:74793806-74793825 | MsG0780040240.01.T01:CDS | 40.0% | |
| CATTGTAGCCATTTCCTCAA+TGG | - | Chr7:74793808-74793827 | None:intergenic | 40.0% | |
| !! | CAATGGCTATTGGTTCACAT+AGG | + | Chr7:74793745-74793764 | MsG0780040240.01.T01:CDS | 40.0% |
| CTATTCAGTGCCGCAAGTAT+AGG | + | Chr7:74793649-74793668 | MsG0780040240.01.T01:CDS | 45.0% | |
| TCACATAGGTTGTCTCAGCA+AGG | + | Chr7:74793759-74793778 | MsG0780040240.01.T01:CDS | 45.0% | |
| TGAGGAAATGGCTACAATGG+TGG | + | Chr7:74793809-74793828 | MsG0780040240.01.T01:CDS | 45.0% | |
| ! | CTATACTTGCGGCACTGAAT+AGG | - | Chr7:74793651-74793670 | None:intergenic | 45.0% |
| ! | CTATTGGTGCTTCTGATTGG+AGG | + | Chr7:74793687-74793706 | MsG0780040240.01.T01:CDS | 45.0% |
| CAGTGCCGCAAGTATAGGAG+TGG | + | Chr7:74793654-74793673 | MsG0780040240.01.T01:CDS | 55.0% | |
| GGCTACAATGGTGGCTGCAA+TGG | + | Chr7:74793818-74793837 | MsG0780040240.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 74793642 | 74793890 | 74793642 | ID=MsG0780040240.01;Name=MsG0780040240.01 |
| Chr7 | mRNA | 74793642 | 74793890 | 74793642 | ID=MsG0780040240.01.T01;Parent=MsG0780040240.01;Name=MsG0780040240.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|82 |
| Chr7 | exon | 74793642 | 74793890 | 74793642 | ID=MsG0780040240.01.T01:exon:14602;Parent=MsG0780040240.01.T01 |
| Chr7 | CDS | 74793642 | 74793890 | 74793642 | ID=MsG0780040240.01.T01:cds;Parent=MsG0780040240.01.T01 |
| Gene Sequence |
| Protein sequence |
