AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000470.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000470.01.T01 MTR_1g015185 77.647 85 15 1 1 81 179 263 2.55e-35 120
MsG0180000470.01.T01 MTR_8g019520 86.667 60 7 1 23 81 197 256 8.21e-33 114
MsG0180000470.01.T01 MTR_8g019520 86.667 60 7 1 23 81 198 257 8.39e-33 114
MsG0180000470.01.T01 MTR_2g099500 86.207 58 7 1 24 80 193 250 1.14e-29 105
MsG0180000470.01.T01 MTR_4g015830 67.089 79 25 1 4 81 162 240 1.38e-28 102
MsG0180000470.01.T01 MTR_2g040990 82.143 56 8 1 28 81 198 253 4.14e-28 101
MsG0180000470.01.T01 MTR_4g134510 79.245 53 11 0 28 80 202 254 9.43e-27 98.2
MsG0180000470.01.T01 MTR_7g068590 75.000 56 14 0 26 81 187 242 1.16e-26 97.8
MsG0180000470.01.T01 MTR_8g066480 54.545 55 12 2 28 81 104 146 4.10e-14 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000470.01.T01 AT5G20510 86.441 59 8 0 23 81 161 219 6.76e-35 118
MsG0180000470.01.T01 AT5G20510 86.441 59 8 0 23 81 202 260 1.34e-34 118
MsG0180000470.01.T01 AT3G42790 89.655 58 6 0 23 80 192 249 6.72e-34 116
MsG0180000470.01.T01 AT5G26210 87.273 55 7 0 26 80 200 254 3.48e-30 107
MsG0180000470.01.T01 AT2G02470 81.818 55 10 0 27 81 202 256 1.65e-29 105
MsG0180000470.01.T01 AT2G02470 81.818 55 10 0 27 81 193 247 2.01e-29 105
MsG0180000470.01.T01 AT5G05610 59.740 77 31 0 4 80 164 240 3.68e-28 101
MsG0180000470.01.T01 AT5G05610 59.740 77 31 0 4 80 164 240 3.68e-28 101
MsG0180000470.01.T01 AT3G11200 68.966 58 18 0 23 80 188 245 2.47e-26 97.1
MsG0180000470.01.T01 AT3G11200 68.966 58 18 0 23 80 175 232 2.94e-26 96.7
MsG0180000470.01.T01 AT1G14510 73.077 52 14 0 27 78 197 248 1.66e-24 92.4

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATGCAGCTGATGAGTTC+TGG 0.348702 1:+6512235 MsG0180000470.01.T01:CDS
TTGATAAGCTCAGCCCTTGC+CGG 0.386506 1:-6512312 None:intergenic
GCAGTGACATTTGCGAGAAA+TGG 0.391588 1:+6512262 MsG0180000470.01.T01:CDS
GTCAAAGGATGAGGAGGTTG+AGG 0.413379 1:+6512152 MsG0180000470.01.T01:CDS
ATTTGCGAGAAATGGTTTCA+TGG 0.414893 1:+6512270 MsG0180000470.01.T01:CDS
GCTCTCTTGTTGCTGCAAGC+TGG 0.467608 1:-6512348 None:intergenic
CGTGAAGATTACACCGGCAA+GGG 0.539345 1:+6512299 MsG0180000470.01.T01:CDS
AAGGATGAGGAGGTTGAGGA+TGG 0.539444 1:+6512156 MsG0180000470.01.T01:CDS
GCAAATGAAGTCAAAGGATG+AGG 0.555951 1:+6512143 MsG0180000470.01.T01:CDS
GAGGAGGTTGAGGATGGTGT+AGG 0.560610 1:+6512162 MsG0180000470.01.T01:CDS
GGTTGAGGATGGTGTAGGTG+TGG 0.578977 1:+6512167 MsG0180000470.01.T01:CDS
GGCAATGCAAATGAAGTCAA+AGG 0.581983 1:+6512137 MsG0180000470.01.T01:CDS
GCGTGAAGATTACACCGGCA+AGG 0.599177 1:+6512298 MsG0180000470.01.T01:CDS
GAGACTTTGTGTGGGGCATG+TGG 0.613065 1:+6512207 MsG0180000470.01.T01:CDS
AACACGGAGAGACTTTGTGT+GGG 0.622939 1:+6512199 MsG0180000470.01.T01:CDS
GGATGGTGTAGGTGTGGACG+AGG 0.628142 1:+6512173 MsG0180000470.01.T01:CDS
GAACACGGAGAGACTTTGTG+TGG 0.630911 1:+6512198 MsG0180000470.01.T01:CDS
AATGAAGTCAAAGGATGAGG+AGG 0.658720 1:+6512146 MsG0180000470.01.T01:CDS
ACACGGAGAGACTTTGTGTG+GGG 0.707279 1:+6512200 MsG0180000470.01.T01:CDS
GGTGTGGACGAGGAAGAACA+CGG 0.729144 1:+6512183 MsG0180000470.01.T01:CDS
AAAATGCGTGAAGATTACAC+CGG 0.768073 1:+6512293 MsG0180000470.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAATGCGTGAAGATTACAC+CGG + Chr1:6512293-6512312 MsG0180000470.01.T01:CDS 35.0%
ATTTGCGAGAAATGGTTTCA+TGG + Chr1:6512270-6512289 MsG0180000470.01.T01:CDS 35.0%
AATGAAGTCAAAGGATGAGG+AGG + Chr1:6512146-6512165 MsG0180000470.01.T01:CDS 40.0%
GCAAATGAAGTCAAAGGATG+AGG + Chr1:6512143-6512162 MsG0180000470.01.T01:CDS 40.0%
GGCAATGCAAATGAAGTCAA+AGG + Chr1:6512137-6512156 MsG0180000470.01.T01:CDS 40.0%
AACACGGAGAGACTTTGTGT+GGG + Chr1:6512199-6512218 MsG0180000470.01.T01:CDS 45.0%
GCAGTGACATTTGCGAGAAA+TGG + Chr1:6512262-6512281 MsG0180000470.01.T01:CDS 45.0%
AAGGATGAGGAGGTTGAGGA+TGG + Chr1:6512156-6512175 MsG0180000470.01.T01:CDS 50.0%
ACACGGAGAGACTTTGTGTG+GGG + Chr1:6512200-6512219 MsG0180000470.01.T01:CDS 50.0%
CGTGAAGATTACACCGGCAA+GGG + Chr1:6512299-6512318 MsG0180000470.01.T01:CDS 50.0%
GAACACGGAGAGACTTTGTG+TGG + Chr1:6512198-6512217 MsG0180000470.01.T01:CDS 50.0%
GCTATGCAGCTGATGAGTTC+TGG + Chr1:6512235-6512254 MsG0180000470.01.T01:CDS 50.0%
GTCAAAGGATGAGGAGGTTG+AGG + Chr1:6512152-6512171 MsG0180000470.01.T01:CDS 50.0%
TTGATAAGCTCAGCCCTTGC+CGG - Chr1:6512315-6512334 None:intergenic 50.0%
GAGACTTTGTGTGGGGCATG+TGG + Chr1:6512207-6512226 MsG0180000470.01.T01:CDS 55.0%
GAGGAGGTTGAGGATGGTGT+AGG + Chr1:6512162-6512181 MsG0180000470.01.T01:CDS 55.0%
GCGTGAAGATTACACCGGCA+AGG + Chr1:6512298-6512317 MsG0180000470.01.T01:CDS 55.0%
GCTCTCTTGTTGCTGCAAGC+TGG - Chr1:6512351-6512370 None:intergenic 55.0%
GGTGTGGACGAGGAAGAACA+CGG + Chr1:6512183-6512202 MsG0180000470.01.T01:CDS 55.0%
GGTTGAGGATGGTGTAGGTG+TGG + Chr1:6512167-6512186 MsG0180000470.01.T01:CDS 55.0%
GGATGGTGTAGGTGTGGACG+AGG + Chr1:6512173-6512192 MsG0180000470.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 6512135 6512380 6512135 ID=MsG0180000470.01;Name=MsG0180000470.01
Chr1 mRNA 6512135 6512380 6512135 ID=MsG0180000470.01.T01;Parent=MsG0180000470.01;Name=MsG0180000470.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|81
Chr1 exon 6512135 6512380 6512135 ID=MsG0180000470.01.T01:exon:2492;Parent=MsG0180000470.01.T01
Chr1 CDS 6512135 6512380 6512135 ID=MsG0180000470.01.T01:cds;Parent=MsG0180000470.01.T01
Gene Sequence

>MsG0180000470.01.T01

ATGGCAATGCAAATGAAGTCAAAGGATGAGGAGGTTGAGGATGGTGTAGGTGTGGACGAGGAAGAACACGGAGAGACTTTGTGTGGGGCATGTGGAGACAGCTATGCAGCTGATGAGTTCTGGATTTGCAGTGACATTTGCGAGAAATGGTTTCATGGAAAATGCGTGAAGATTACACCGGCAAGGGCTGAGCTTATCAAACAGTACAAATGTCCAGCTTGCAGCAACAAGAGAGCTCGCCCTTGA

Protein sequence

>MsG0180000470.01.T01

MAMQMKSKDEEVEDGVGVDEEEHGETLCGACGDSYAADEFWICSDICEKWFHGKCVKITPARAELIKQYKCPACSNKRARP*