Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040423.01.T01 | XP_003625033.1 | 88.462 | 182 | 13 | 3 | 1 | 174 | 1 | 182 | 7.58E-108 | 318 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040423.01.T01 | Q9M1J2 | 47.5 | 160 | 76 | 2 | 19 | 174 | 1 | 156 | 1.91E-48 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040423.01.T01 | A2Q2Z5 | 88.462 | 182 | 13 | 3 | 1 | 174 | 1 | 182 | 3.62e-108 | 318 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580024084.01 | MsG0780040423.01 | PPI |
| MsG0580024772.01 | MsG0780040423.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040423.01.T01 | MTR_7g090220 | 88.462 | 182 | 13 | 3 | 1 | 174 | 1 | 182 | 9.18e-112 | 318 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040423.01.T01 | AT3G57080 | 47.500 | 160 | 76 | 2 | 19 | 174 | 1 | 156 | 1.95e-49 | 159 |
| MsG0780040423.01.T01 | AT2G41340 | 55.200 | 125 | 56 | 0 | 40 | 164 | 14 | 138 | 1.11e-47 | 153 |
| MsG0780040423.01.T01 | AT2G41340 | 55.200 | 125 | 56 | 0 | 40 | 164 | 14 | 138 | 1.60e-47 | 154 |
| MsG0780040423.01.T01 | AT2G41340 | 55.200 | 125 | 56 | 0 | 40 | 164 | 14 | 138 | 3.33e-47 | 153 |
| MsG0780040423.01.T01 | AT3G54490 | 40.769 | 130 | 77 | 0 | 35 | 164 | 24 | 153 | 3.69e-30 | 110 |
Find 32 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGAGATTGGCCATGAATT+TGG | 0.226350 | 7:-77371140 | None:intergenic |
| AGAGATATGGCGACTGAAAA+TGG | 0.293092 | 7:+77370892 | MsG0780040423.01.T01:exon |
| CATCGTTGTTATTGCTGTTT+CGG | 0.317384 | 7:-77370932 | None:intergenic |
| GATGATTTCCGCCAAATTCA+TGG | 0.323871 | 7:+77371129 | MsG0780040423.01.T01:CDS |
| TTGAGACAAGAGTTAATAAT+AGG | 0.323906 | 7:+77370823 | None:intergenic |
| TGTTAATCTTCTCTCTTTCA+AGG | 0.372770 | 7:+77371461 | MsG0780040423.01.T01:CDS |
| ATCAAAGAGAGAGACCCTTT+TGG | 0.387197 | 7:-77371208 | None:intergenic |
| TTGTTCACCAAGATGGATAA+TGG | 0.412324 | 7:+77371003 | MsG0780040423.01.T01:CDS |
| TATGCTTCCATTATCCATCT+TGG | 0.423538 | 7:-77371010 | None:intergenic |
| ATGCCACTAACCCCTCCAAA+AGG | 0.454191 | 7:+77371193 | MsG0780040423.01.T01:CDS |
| GACGATCAACATCAGGAGAT+TGG | 0.475030 | 7:-77371152 | None:intergenic |
| TAAGTGTTTGTTCACCAAGA+TGG | 0.482415 | 7:+77370996 | MsG0780040423.01.T01:CDS |
| CCAGGGATAGTGAAAGTGAA+TGG | 0.482754 | 7:+77371342 | MsG0780040423.01.T01:CDS |
| CAGGGATAGTGAAAGTGAAT+GGG | 0.483393 | 7:+77371343 | MsG0780040423.01.T01:CDS |
| CCATTCACTTTCACTATCCC+TGG | 0.484269 | 7:-77371342 | None:intergenic |
| ACAACGATGGAGATCGAAAA+CGG | 0.484630 | 7:+77370946 | MsG0780040423.01.T01:CDS |
| ACTGAAAATGGAGGACAAAA+CGG | 0.521986 | 7:+77370904 | MsG0780040423.01.T01:CDS |
| GTGAACAGAGACAGTTTGAC+TGG | 0.526473 | 7:+77371390 | MsG0780040423.01.T01:CDS |
| CGTTGTTATTGCTGTTTCGG+TGG | 0.535026 | 7:-77370929 | None:intergenic |
| TGGCATGAGTAGCAGTAAAA+CGG | 0.535822 | 7:-77371176 | None:intergenic |
| GAGATTGGCCATGAATTTGG+CGG | 0.538467 | 7:-77371137 | None:intergenic |
| GAGAGCTGGATTTCATCAGA+AGG | 0.539635 | 7:-77371102 | None:intergenic |
| AAACGGAGACGATCAACATC+AGG | 0.547032 | 7:-77371159 | None:intergenic |
| TGCCACTAACCCCTCCAAAA+GGG | 0.558127 | 7:+77371194 | MsG0780040423.01.T01:CDS |
| GGAAATCATCAAGAGAGAGC+TGG | 0.605433 | 7:-77371116 | None:intergenic |
| ATCTTGGTGAACAAACACTT+AGG | 0.617123 | 7:-77370994 | None:intergenic |
| AGAGCTGGATTTCATCAGAA+GGG | 0.630001 | 7:-77371101 | None:intergenic |
| CGAAACAGCAATAACAACGA+TGG | 0.637396 | 7:+77370933 | MsG0780040423.01.T01:CDS |
| AATGGGGTAAGAGACATAGC+AGG | 0.645159 | 7:+77371360 | MsG0780040423.01.T01:CDS |
| CTTGAGATGCTCAAAGACAG+AGG | 0.664708 | 7:+77371069 | MsG0780040423.01.T01:CDS |
| AGGGATAGTGAAAGTGAATG+GGG | 0.668631 | 7:+77371344 | MsG0780040423.01.T01:CDS |
| GATATGGCGACTGAAAATGG+AGG | 0.750626 | 7:+77370895 | MsG0780040423.01.T01:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTATATGAAAACT+AGG | + | Chr7:77371247-77371266 | MsG0780040423.01.T01:intron | 10.0% |
| ! | TTATATGAAAACTAGGTATC+TGG | + | Chr7:77371254-77371273 | MsG0780040423.01.T01:intron | 25.0% |
| TGTTAATCTTCTCTCTTTCA+AGG | + | Chr7:77371461-77371480 | MsG0780040423.01.T01:CDS | 30.0% | |
| ! | CTTTCAAGGTTGAAATTTTC+AGG | + | Chr7:77371475-77371494 | MsG0780040423.01.T01:CDS | 30.0% |
| !! | TAATGAGTGTTTGAGTTGTT+GGG | + | Chr7:77371290-77371309 | MsG0780040423.01.T01:intron | 30.0% |
| !!! | AGATTTTGGTGGTTTTTAGT+GGG | + | Chr7:77371319-77371338 | MsG0780040423.01.T01:intron | 30.0% |
| ACTGAAAATGGAGGACAAAA+CGG | + | Chr7:77370904-77370923 | MsG0780040423.01.T01:CDS | 35.0% | |
| ATCTTGGTGAACAAACACTT+AGG | - | Chr7:77370997-77371016 | None:intergenic | 35.0% | |
| CAACTCAAACACTCATTAGT+CGG | - | Chr7:77371288-77371307 | None:intergenic | 35.0% | |
| TAAGTGTTTGTTCACCAAGA+TGG | + | Chr7:77370996-77371015 | MsG0780040423.01.T01:CDS | 35.0% | |
| TATGCTTCCATTATCCATCT+TGG | - | Chr7:77371013-77371032 | None:intergenic | 35.0% | |
| TTGTTCACCAAGATGGATAA+TGG | + | Chr7:77371003-77371022 | MsG0780040423.01.T01:CDS | 35.0% | |
| ! | TTGTTGGGTTTTGCAGATTT+TGG | + | Chr7:77371305-77371324 | MsG0780040423.01.T01:intron | 35.0% |
| !! | CAGATTTTGGTGGTTTTTAG+TGG | + | Chr7:77371318-77371337 | MsG0780040423.01.T01:intron | 35.0% |
| !! | CATCGTTGTTATTGCTGTTT+CGG | - | Chr7:77370935-77370954 | None:intergenic | 35.0% |
| !! | CTAATGAGTGTTTGAGTTGT+TGG | + | Chr7:77371289-77371308 | MsG0780040423.01.T01:intron | 35.0% |
| ACAACGATGGAGATCGAAAA+CGG | + | Chr7:77370946-77370965 | MsG0780040423.01.T01:CDS | 40.0% | |
| AGAGATATGGCGACTGAAAA+TGG | + | Chr7:77370892-77370911 | MsG0780040423.01.T01:exon | 40.0% | |
| AGAGCTGGATTTCATCAGAA+GGG | - | Chr7:77371104-77371123 | None:intergenic | 40.0% | |
| AGGGATAGTGAAAGTGAATG+GGG | + | Chr7:77371344-77371363 | MsG0780040423.01.T01:CDS | 40.0% | |
| CAGGGATAGTGAAAGTGAAT+GGG | + | Chr7:77371343-77371362 | MsG0780040423.01.T01:CDS | 40.0% | |
| CGAAACAGCAATAACAACGA+TGG | + | Chr7:77370933-77370952 | MsG0780040423.01.T01:CDS | 40.0% | |
| GAGATAGAGAGAGAGAGATA+TGG | + | Chr7:77370879-77370898 | MsG0780040423.01.T01:exon | 40.0% | |
| GATGATTTCCGCCAAATTCA+TGG | + | Chr7:77371129-77371148 | MsG0780040423.01.T01:CDS | 40.0% | |
| TGGCATGAGTAGCAGTAAAA+CGG | - | Chr7:77371179-77371198 | None:intergenic | 40.0% | |
| ! | AAAACTAGGTATCTGGCTCA+AGG | + | Chr7:77371261-77371280 | MsG0780040423.01.T01:intron | 40.0% |
| ! | ATCAAAGAGAGAGACCCTTT+TGG | - | Chr7:77371211-77371230 | None:intergenic | 40.0% |
| ! | TTGGGTTTTGCAGATTTTGG+TGG | + | Chr7:77371308-77371327 | MsG0780040423.01.T01:intron | 40.0% |
| AAACGGAGACGATCAACATC+AGG | - | Chr7:77371162-77371181 | None:intergenic | 45.0% | |
| AATGGGGTAAGAGACATAGC+AGG | + | Chr7:77371360-77371379 | MsG0780040423.01.T01:CDS | 45.0% | |
| CCAGGGATAGTGAAAGTGAA+TGG | + | Chr7:77371342-77371361 | MsG0780040423.01.T01:CDS | 45.0% | |
| CCATTCACTTTCACTATCCC+TGG | - | Chr7:77371345-77371364 | None:intergenic | 45.0% | |
| CTTGAGATGCTCAAAGACAG+AGG | + | Chr7:77371069-77371088 | MsG0780040423.01.T01:CDS | 45.0% | |
| GACGATCAACATCAGGAGAT+TGG | - | Chr7:77371155-77371174 | None:intergenic | 45.0% | |
| GAGAGCTGGATTTCATCAGA+AGG | - | Chr7:77371105-77371124 | None:intergenic | 45.0% | |
| GATATGGCGACTGAAAATGG+AGG | + | Chr7:77370895-77370914 | MsG0780040423.01.T01:exon | 45.0% | |
| GGAAATCATCAAGAGAGAGC+TGG | - | Chr7:77371119-77371138 | None:intergenic | 45.0% | |
| GTGAACAGAGACAGTTTGAC+TGG | + | Chr7:77371390-77371409 | MsG0780040423.01.T01:CDS | 45.0% | |
| ! | AAAGAGAGAGACCCTTTTGG+AGG | - | Chr7:77371208-77371227 | None:intergenic | 45.0% |
| ! | AAGAGAGAGACCCTTTTGGA+GGG | - | Chr7:77371207-77371226 | None:intergenic | 45.0% |
| ! | CAGGAGATTGGCCATGAATT+TGG | - | Chr7:77371143-77371162 | None:intergenic | 45.0% |
| ! | GAGATTGGCCATGAATTTGG+CGG | - | Chr7:77371140-77371159 | None:intergenic | 45.0% |
| !! | CGTTGTTATTGCTGTTTCGG+TGG | - | Chr7:77370932-77370951 | None:intergenic | 45.0% |
| ATGCCACTAACCCCTCCAAA+AGG | + | Chr7:77371193-77371212 | MsG0780040423.01.T01:CDS | 50.0% | |
| TGCCACTAACCCCTCCAAAA+GGG | + | Chr7:77371194-77371213 | MsG0780040423.01.T01:CDS | 50.0% | |
| !! | AGAGAGAGACCCTTTTGGAG+GGG | - | Chr7:77371206-77371225 | None:intergenic | 50.0% |
| !!! | TGGTGGTTTTTAGTGGGCCA+GGG | + | Chr7:77371325-77371344 | MsG0780040423.01.T01:CDS | 50.0% |
| !!! | TTGGTGGTTTTTAGTGGGCC+AGG | + | Chr7:77371324-77371343 | MsG0780040423.01.T01:CDS | 50.0% |
| !! | GACCCTTTTGGAGGGGTTAG+TGG | - | Chr7:77371199-77371218 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 77370825 | 77371533 | 77370825 | ID=MsG0780040423.01;Name=MsG0780040423.01 |
| Chr7 | mRNA | 77370825 | 77371533 | 77370825 | ID=MsG0780040423.01.T01;Parent=MsG0780040423.01;Name=MsG0780040423.01.T01;_AED=0.26;_eAED=0.26;_QI=73|1|0.5|1|1|1|2|0|176 |
| Chr7 | exon | 77370825 | 77371215 | 77370825 | ID=MsG0780040423.01.T01:exon:15289;Parent=MsG0780040423.01.T01 |
| Chr7 | exon | 77371321 | 77371533 | 77371321 | ID=MsG0780040423.01.T01:exon:15290;Parent=MsG0780040423.01.T01 |
| Chr7 | five_prime_UTR | 77370825 | 77370897 | 77370825 | ID=MsG0780040423.01.T01:five_prime_utr;Parent=MsG0780040423.01.T01 |
| Chr7 | CDS | 77370898 | 77371215 | 77370898 | ID=MsG0780040423.01.T01:cds;Parent=MsG0780040423.01.T01 |
| Chr7 | CDS | 77371321 | 77371533 | 77371321 | ID=MsG0780040423.01.T01:cds;Parent=MsG0780040423.01.T01 |
| Gene Sequence |
| Protein sequence |
