AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040434.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040434.01.T01 MTR_7g090050 97.992 249 4 1 1 248 1 249 0.0 504
MsG0780040434.01.T01 MTR_7g090050 87.770 278 4 2 1 248 1 278 8.00e-178 489
MsG0780040434.01.T01 MTR_7g090050 98.830 171 2 0 78 248 36 206 8.16e-126 355
MsG0780040434.01.T01 MTR_5g023210 45.181 166 91 0 56 221 20 185 1.19e-54 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040434.01.T01 AT3G10210 69.198 237 70 2 4 238 2 237 9.79e-117 333
MsG0780040434.01.T01 AT4G35750 45.455 198 108 0 38 235 2 199 1.99e-59 187
MsG0780040434.01.T01 AT1G69340 25.882 170 121 4 32 199 374 540 3.44e-12 66.2
MsG0780040434.01.T01 AT1G69340 25.882 170 121 4 32 199 374 540 3.44e-12 66.2

Find 131 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATAAATTGCTACGAAATT+AGG 0.177491 7:-77465491 None:intergenic
ACAAAAGCATGATGATATTT+TGG 0.180310 7:+77464845 MsG0780040434.01.T01:CDS
CCTCTTCTTCTTGCTGTTCA+TGG 0.184887 7:-77463535 None:intergenic
AGATCTTCCAGATGAATTTA+AGG 0.194706 7:+77464425 MsG0780040434.01.T01:CDS
TCCACCCTGGGCTTCGTTCC+AGG 0.224124 7:+77464472 MsG0780040434.01.T01:CDS
ATCTTTAGCAGGAGAGCTTT+TGG 0.249199 7:+77465629 MsG0780040434.01.T01:three_prime_UTR
AATAGGCCACTCACTGATTA+TGG 0.278886 7:+77464870 MsG0780040434.01.T01:CDS
TTTCAGACATAAGAATAATA+AGG 0.306158 7:-77465375 None:intergenic
GATCTTTGTTTAGATTATAT+TGG 0.322681 7:+77464745 MsG0780040434.01.T01:intron
CAACTTTCTGATTGCAACCT+TGG 0.334312 7:+77465696 MsG0780040434.01.T01:three_prime_UTR
TTTCACATCATGTGGGGTTA+TGG 0.340176 7:+77465820 MsG0780040434.01.T01:three_prime_UTR
TGCACGTAAACGATGCAAAA+TGG 0.346344 7:-77464342 None:intergenic
TGTGCATATACTTGCCCCTT+TGG 0.353391 7:+77465219 MsG0780040434.01.T01:three_prime_UTR
CTCTGTGTTATTTACTGTGC+AGG 0.357663 7:-77463421 None:intergenic
CCTTCAGTTGCATACTCGTT+TGG 0.359573 7:+77464927 MsG0780040434.01.T01:CDS
GTTGGTGTCAATTGGAGAAT+TGG 0.360378 7:+77465567 MsG0780040434.01.T01:three_prime_UTR
CCCGACCCCTTTCACTTGAC+TGG 0.361568 7:+77464900 MsG0780040434.01.T01:CDS
GTGCATATACTTGCCCCTTT+GGG 0.384172 7:+77465220 MsG0780040434.01.T01:three_prime_UTR
AGCCTGTCCTTAAATTCATC+TGG 0.389216 7:-77464432 None:intergenic
CAAGAGACCGAGTGTATTGT+TGG 0.394593 7:-77465940 None:intergenic
TAAGCCGCCTTCAGTACCTT+TGG 0.400237 7:+77464781 MsG0780040434.01.T01:CDS
ATACATCTTTGAATATTCAT+TGG 0.407908 7:+77465193 MsG0780040434.01.T01:three_prime_UTR
CTTCTTGCTGTTCATGGAGA+AGG 0.417496 7:-77463529 None:intergenic
ACGATGCAAAATGGCCCATC+TGG 0.420128 7:-77464333 None:intergenic
TGGAGAACAAGGTTGAATAT+TGG 0.420487 7:+77465716 MsG0780040434.01.T01:three_prime_UTR
AAGTATGAAGAGAGATGGGC+AGG 0.422356 7:+77464951 MsG0780040434.01.T01:CDS
ACAAGCCTGGAACGAAGCCC+AGG 0.422750 7:-77464477 None:intergenic
GAATTGGCAAATTGTTGTTA+AGG 0.424311 7:+77465583 MsG0780040434.01.T01:three_prime_UTR
AGGCTTGTCATGGCTACTCT+TGG 0.428020 7:+77464492 MsG0780040434.01.T01:CDS
ACGAATGAGGATAATTCTCC+TGG 0.432791 7:+77464375 MsG0780040434.01.T01:CDS
ATGAAGAGAGATGGGCAGGA+AGG 0.433561 7:+77464955 MsG0780040434.01.T01:CDS
TTGTCATGGCTACTCTTGGC+AGG 0.435525 7:+77464496 MsG0780040434.01.T01:CDS
AAGCCGCCTTCAGTACCTTT+GGG 0.439554 7:+77464782 MsG0780040434.01.T01:CDS
TTGAATATTGGTGTTGGAGG+CGG 0.443086 7:+77465728 MsG0780040434.01.T01:three_prime_UTR
GGAGGCGGTATTGTGGATAA+AGG 0.444448 7:+77465743 MsG0780040434.01.T01:three_prime_UTR
AATGAAGAACTTCTCTTCAA+AGG 0.459108 7:-77465904 None:intergenic
CCGTGCATGCTATCTATTGT+TGG 0.459232 7:+77465257 MsG0780040434.01.T01:three_prime_UTR
GTTGGTTAATTAGAGAGGAT+TGG 0.468501 7:+77465797 MsG0780040434.01.T01:three_prime_UTR
GGCATCCCAGTCAAGTGAAA+GGG 0.475353 7:-77464906 None:intergenic
GTTGTTAAGGAAGAAATCCT+TGG 0.476905 7:+77465596 MsG0780040434.01.T01:three_prime_UTR
ACAAGGTTGAATATTGGTGT+TGG 0.488150 7:+77465722 MsG0780040434.01.T01:three_prime_UTR
CCGACCCCTTTCACTTGACT+GGG 0.497649 7:+77464901 MsG0780040434.01.T01:CDS
AAGAGTAGCCATGACAAGCC+TGG 0.504024 7:-77464490 None:intergenic
CACACAGCTTTATACCCAAA+GGG 0.504749 7:-77465234 None:intergenic
GTCACCTGGTATATAGTTTG+AGG 0.505446 7:+77465328 MsG0780040434.01.T01:three_prime_UTR
TTCCAGATGAATTTAAGGAC+AGG 0.506244 7:+77464430 MsG0780040434.01.T01:CDS
GTGAAGGGAAAATCTTTAGC+AGG 0.507670 7:+77465618 MsG0780040434.01.T01:three_prime_UTR
CATAACATGTAGTCACGTAC+AGG 0.509444 7:-77465112 None:intergenic
CTTTGTACTTCATCCACCCT+GGG 0.512403 7:+77464460 MsG0780040434.01.T01:CDS
GCTTCGTTCCAGGCTTGTCA+TGG 0.516062 7:+77464482 MsG0780040434.01.T01:CDS
CCCAGTCAAGTGAAAGGGGT+CGG 0.517168 7:-77464901 None:intergenic
AAGGAAAGGTCGGGCATCGA+CGG 0.519151 7:-77463510 None:intergenic
TCTGTGTTATTTACTGTGCA+GGG 0.519613 7:-77463420 None:intergenic
AGATCGGAGAAGTCTTCGTC+GGG 0.520373 7:-77463587 None:intergenic
TTACAATTCTTCACTCTCCA+AGG 0.520897 7:+77463617 MsG0780040434.01.T01:CDS
CCAGTCAAGTGAAAGGGGTC+GGG 0.521707 7:-77464900 None:intergenic
ACGAACTATTCATCATGTAC+TGG 0.528343 7:-77465452 None:intergenic
TTTACTGTGCAGGGTGGGTG+TGG 0.528704 7:-77463411 None:intergenic
TAACAGTTGGTTAATTAGAG+AGG 0.529883 7:+77465792 MsG0780040434.01.T01:three_prime_UTR
ATTCAAAGGAAAGGCAAGGA+AGG 0.530306 7:+77465428 MsG0780040434.01.T01:three_prime_UTR
TTGGAAAGTATGAAGAGAGA+TGG 0.530944 7:+77464946 MsG0780040434.01.T01:CDS
AGGTTGAATATTGGTGTTGG+AGG 0.531452 7:+77465725 MsG0780040434.01.T01:three_prime_UTR
CTTTGGGATGATATAAAGAA+AGG 0.533094 7:+77464798 MsG0780040434.01.T01:CDS
TGTTATTTACTGTGCAGGGT+GGG 0.535219 7:-77463416 None:intergenic
AGGATTGGTTTCACATCATG+TGG 0.535443 7:+77465812 MsG0780040434.01.T01:three_prime_UTR
TGAAGAGAGATGGGCAGGAA+GGG 0.538035 7:+77464956 MsG0780040434.01.T01:CDS
ATTTATGGTCAAGTAACAGT+TGG 0.539791 7:+77465779 MsG0780040434.01.T01:three_prime_UTR
CACCTCAAGATTGTCATCCC+AGG 0.540229 7:-77464393 None:intergenic
CCATGAACAGCAAGAAGAAG+AGG 0.542131 7:+77463535 MsG0780040434.01.T01:CDS
AAGGAAGAAATCCTTGGTGA+AGG 0.543698 7:+77465602 MsG0780040434.01.T01:three_prime_UTR
CAAGGTTGCAATCAGAAAGT+TGG 0.544924 7:-77465695 None:intergenic
TGCTGTTCATGGAGAAGGAA+AGG 0.547148 7:-77463524 None:intergenic
AGAAGTCTTCGTCGGGAGAG+AGG 0.547729 7:-77463580 None:intergenic
AACAGCAAGAAGAAGAGGAT+TGG 0.548513 7:+77463540 MsG0780040434.01.T01:CDS
CTCAGTGGTGGTCCTAGCAT+CGG 0.550386 7:+77463481 MsG0780040434.01.T01:CDS
ATATTCAACCTTGTTCTCCA+AGG 0.556116 7:-77465713 None:intergenic
AGGAAGAAATCCTTGGTGAA+GGG 0.556162 7:+77465603 MsG0780040434.01.T01:three_prime_UTR
CGACGGCGAGATCCGATGCT+AGG 0.557306 7:-77463493 None:intergenic
ACATCATGTGGGGTTATGGA+AGG 0.565381 7:+77465824 MsG0780040434.01.T01:three_prime_UTR
TGGTGTTGGAGGCGGTATTG+TGG 0.567022 7:+77465736 MsG0780040434.01.T01:three_prime_UTR
GCACAGCACTGTTACGAATG+AGG 0.569439 7:+77464362 MsG0780040434.01.T01:CDS
ATGCCTAAAATTCAAAGGAA+AGG 0.571843 7:+77465419 MsG0780040434.01.T01:three_prime_UTR
TGAGTGCAGAGCGGCTGAAG+AGG 0.572893 7:+77464280 MsG0780040434.01.T01:CDS
GATTGCAACCTTGGAGAACA+AGG 0.575899 7:+77465705 MsG0780040434.01.T01:three_prime_UTR
GTTCATGGAGAAGGAAAGGT+CGG 0.577646 7:-77463520 None:intergenic
CTTTCTTTATATCATCCCAA+AGG 0.578331 7:-77464797 None:intergenic
ACTTTGTACTTCATCCACCC+TGG 0.579241 7:+77464459 MsG0780040434.01.T01:CDS
TTGCCATGCCTAAAATTCAA+AGG 0.580607 7:+77465414 MsG0780040434.01.T01:three_prime_UTR
TGGAAAGTATGAAGAGAGAT+GGG 0.582285 7:+77464947 MsG0780040434.01.T01:CDS
ACTCGCATTCTCCGCATCGT+TGG 0.583897 7:+77463656 MsG0780040434.01.T01:CDS
TTATTCAGTGAGTTACCAGA+TGG 0.588230 7:+77464318 MsG0780040434.01.T01:CDS
AGGCATCCCAGTCAAGTGAA+AGG 0.589065 7:-77464907 None:intergenic
TATCATCCCAAAGGTACTGA+AGG 0.594807 7:-77464788 None:intergenic
GGATTGGTTTCACATCATGT+GGG 0.596545 7:+77465813 MsG0780040434.01.T01:three_prime_UTR
GCACACAGCTTTATACCCAA+AGG 0.597133 7:-77465235 None:intergenic
ATCGGAGGAATTCTCAGTGG+TGG 0.599361 7:+77463469 MsG0780040434.01.T01:CDS
CTGGGATGACAATCTTGAGG+TGG 0.602184 7:+77464394 MsG0780040434.01.T01:CDS
GTGTTATTTACTGTGCAGGG+TGG 0.602764 7:-77463417 None:intergenic
TAAAATTCAAAGGAAAGGCA+AGG 0.602854 7:+77465424 MsG0780040434.01.T01:three_prime_UTR
TTCAAAGGAAAGGCAAGGAA+GGG 0.603319 7:+77465429 MsG0780040434.01.T01:three_prime_UTR
CTCCTGGGATGACAATCTTG+AGG 0.605207 7:+77464391 MsG0780040434.01.T01:CDS
TTCATGGAGAAGGAAAGGTC+GGG 0.605657 7:-77463519 None:intergenic
CATGATGAATAGTTCGTGAA+GGG 0.606500 7:+77465458 MsG0780040434.01.T01:three_prime_UTR
ATCGTTGGAAAACACTATCC+CGG 0.608247 7:+77463671 MsG0780040434.01.T01:CDS
ACATGATGAATAGTTCGTGA+AGG 0.611645 7:+77465457 MsG0780040434.01.T01:three_prime_UTR
CAAGCCTGGAACGAAGCCCA+GGG 0.612500 7:-77464476 None:intergenic
GGTATTGTGGATAAAGGACA+AGG 0.617588 7:+77465749 MsG0780040434.01.T01:three_prime_UTR
GAGATCGGAGAAGTCTTCGT+CGG 0.619875 7:-77463588 None:intergenic
GAACGCTTCTATATGTCACC+TGG 0.622652 7:+77465314 MsG0780040434.01.T01:three_prime_UTR
TTAGGTACGCTGTACACAAG+TGG 0.623584 7:-77465023 None:intergenic
GAATTGTAAGAATTCGAGAT+CGG 0.623968 7:-77463603 None:intergenic
CGAATGAGGATAATTCTCCT+GGG 0.628036 7:+77464376 MsG0780040434.01.T01:CDS
AAAGGCGGCGATGAGTTCAT+CGG 0.629506 7:+77463451 MsG0780040434.01.T01:exon
GCATCCCAGTCAAGTGAAAG+GGG 0.635454 7:-77464905 None:intergenic
CATCCCAAAGGTACTGAAGG+CGG 0.635715 7:-77464785 None:intergenic
GATTGGTTTCACATCATGTG+GGG 0.636582 7:+77465814 MsG0780040434.01.T01:three_prime_UTR
CCAAACGAGTATGCAACTGA+AGG 0.637348 7:-77464927 None:intergenic
TAAGTTTCCAACAATACACT+CGG 0.644192 7:+77465933 MsG0780040434.01.T01:three_prime_UTR
ATAACATGTAGTCACGTACA+GGG 0.651104 7:-77465111 None:intergenic
TATTCAGTGAGTTACCAGAT+GGG 0.652106 7:+77464319 MsG0780040434.01.T01:CDS
CCAACAATAGATAGCATGCA+CGG 0.656060 7:-77465257 None:intergenic
AATACCTCAAACTATATACC+AGG 0.666420 7:-77465332 None:intergenic
TCAATTCCATAATCAGTGAG+TGG 0.666908 7:-77464876 None:intergenic
GCCTGGAACGAAGCCCAGGG+TGG 0.672073 7:-77464473 None:intergenic
ACACAGAGAGATAGCAAAGG+CGG 0.679950 7:+77463436 MsG0780040434.01.T01:five_prime_UTR
GGCGGCGATGAGTTCATCGG+AGG 0.685686 7:+77463454 MsG0780040434.01.T01:exon
ACACAGCTTTATACCCAAAG+GGG 0.695191 7:-77465233 None:intergenic
CAACAGTTGTGAGTGCAGAG+CGG 0.698313 7:+77464271 MsG0780040434.01.T01:CDS
ATAACACAGAGAGATAGCAA+AGG 0.714270 7:+77463433 MsG0780040434.01.T01:five_prime_UTR
TTCATCGGAGGAATTCTCAG+TGG 0.740829 7:+77463466 MsG0780040434.01.T01:CDS
GTATTGTGGATAAAGGACAA+GGG 0.758783 7:+77465750 MsG0780040434.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATAAAAATTATACATT+GGG - Chr7:77464694-77464713 None:intergenic 10.0%
!! TAAACATAAAAATTATACAT+TGG - Chr7:77464695-77464714 None:intergenic 10.0%
!! AAATTCAAATGAAAAATAGT+TGG + Chr7:77465549-77465568 MsG0780040434.01.T01:three_prime_UTR 15.0%
!! AGAACATAAAAATATATGTA+TGG + Chr7:77466049-77466068 MsG0780040434.01.T01:three_prime_UTR 15.0%
!! ATTGAACTATTAAAAAGATT+TGG + Chr7:77466009-77466028 MsG0780040434.01.T01:three_prime_UTR 15.0%
!! GAACATAAAAATATATGTAT+GGG + Chr7:77466050-77466069 MsG0780040434.01.T01:three_prime_UTR 15.0%
!!! ACTATTTTTCATTTGAATTT+TGG - Chr7:77465549-77465568 None:intergenic 15.0%
!!! CTATTTTTCATTTGAATTTT+GGG - Chr7:77465548-77465567 None:intergenic 15.0%
!!! TCATTTTTTCATTATATAGT+TGG + Chr7:77465847-77465866 MsG0780040434.01.T01:three_prime_UTR 15.0%
!! AAGTGCTTATGTATAAATTA+TGG - Chr7:77463867-77463886 None:intergenic 20.0%
!! AGTGCTTATGTATAAATTAT+GGG - Chr7:77463866-77463885 None:intergenic 20.0%
!! ATACATCTTTGAATATTCAT+TGG + Chr7:77465193-77465212 MsG0780040434.01.T01:three_prime_UTR 20.0%
!! ATGAAAATATAGATGCAAAA+AGG - Chr7:77464657-77464676 None:intergenic 20.0%
!! ATTAATAAAGAACAGTTTCT+AGG - Chr7:77464209-77464228 None:intergenic 20.0%
!! ATTAATTCTATCTCTTATGT+TGG + Chr7:77464223-77464242 MsG0780040434.01.T01:intron 20.0%
!! GATCATGTTAAACATAATTT+AGG + Chr7:77464056-77464075 MsG0780040434.01.T01:intron 20.0%
!! TAAAAATATATGTATGGGTT+AGG + Chr7:77466055-77466074 MsG0780040434.01.T01:three_prime_UTR 20.0%
!! TTCAGAATTTCATATTATAG+CGG + Chr7:77463745-77463764 MsG0780040434.01.T01:intron 20.0%
!! TTTCAGACATAAGAATAATA+AGG - Chr7:77465378-77465397 None:intergenic 20.0%
!!! ATACAAACACATGATATTTT+AGG - Chr7:77465044-77465063 None:intergenic 20.0%
!!! ATATATTTTTATGTTCTGCA+TGG - Chr7:77466046-77466065 None:intergenic 20.0%
!!! CAATTTTCTTATTTGAACAA+AGG - Chr7:77464131-77464150 None:intergenic 20.0%
!!! GATCTTTGTTTAGATTATAT+TGG + Chr7:77464745-77464764 MsG0780040434.01.T01:intron 20.0%
!!! TATTAAAAAGATTTGGTTCA+TGG + Chr7:77466016-77466035 MsG0780040434.01.T01:three_prime_UTR 20.0%
!!! TGATTTTTGTACAACAAATT+CGG - Chr7:77464025-77464044 None:intergenic 20.0%
!!! TTAAGTTTAAATTTTCTCTC+TGG - Chr7:77464155-77464174 None:intergenic 20.0%
! AAAATTTAAACTTAAACCCC+TGG + Chr7:77464160-77464179 MsG0780040434.01.T01:intron 25.0%
! CATAAAAATTATACATTGGG+TGG - Chr7:77464691-77464710 None:intergenic 25.0%
! CTATAAATTGCTACGAAATT+AGG - Chr7:77465494-77465513 None:intergenic 25.0%
! GTGCTTATGTATAAATTATG+GGG - Chr7:77463865-77463884 None:intergenic 25.0%
! TTGTTACGATAATTATGGTT+AGG + Chr7:77463768-77463787 MsG0780040434.01.T01:intron 25.0%
!!! AAGCATCGAATGAAATTATA+TGG + Chr7:77464602-77464621 MsG0780040434.01.T01:intron 25.0%
!!! ACAAAAGCATGATGATATTT+TGG + Chr7:77464845-77464864 MsG0780040434.01.T01:CDS 25.0%
!!! ATGATGATATTTTGGAGAAT+AGG + Chr7:77464853-77464872 MsG0780040434.01.T01:CDS 25.0%
AATACCTCAAACTATATACC+AGG - Chr7:77465335-77465354 None:intergenic 30.0%
AATGAAGAACTTCTCTTCAA+AGG - Chr7:77465907-77465926 None:intergenic 30.0%
AGATCTTCCAGATGAATTTA+AGG + Chr7:77464425-77464444 MsG0780040434.01.T01:CDS 30.0%
ATAAAACTAAAAGCCCTGTT+TGG - Chr7:77463936-77463955 None:intergenic 30.0%
ATAATGTTGATCATATTGCC+AGG - Chr7:77464181-77464200 None:intergenic 30.0%
ATGCCTAAAATTCAAAGGAA+AGG + Chr7:77465419-77465438 MsG0780040434.01.T01:three_prime_UTR 30.0%
ATTTATGGTCAAGTAACAGT+TGG + Chr7:77465779-77465798 MsG0780040434.01.T01:three_prime_UTR 30.0%
CTTTCTTTATATCATCCCAA+AGG - Chr7:77464800-77464819 None:intergenic 30.0%
GAATTGTAAGAATTCGAGAT+CGG - Chr7:77463606-77463625 None:intergenic 30.0%
GGACAAGGGAAAAATATTTA+TGG + Chr7:77465764-77465783 MsG0780040434.01.T01:three_prime_UTR 30.0%
TAAAATTCAAAGGAAAGGCA+AGG + Chr7:77465424-77465443 MsG0780040434.01.T01:three_prime_UTR 30.0%
TAAACATAATTTAGGCCTCT+TGG + Chr7:77464064-77464083 MsG0780040434.01.T01:intron 30.0%
TAACAGTTGGTTAATTAGAG+AGG + Chr7:77465792-77465811 MsG0780040434.01.T01:three_prime_UTR 30.0%
TAAGTTTCCAACAATACACT+CGG + Chr7:77465933-77465952 MsG0780040434.01.T01:three_prime_UTR 30.0%
TAATGTTGATCATATTGCCA+GGG - Chr7:77464180-77464199 None:intergenic 30.0%
TTCTTAGCAGAAATGTTTAG+AGG + Chr7:77464553-77464572 MsG0780040434.01.T01:intron 30.0%
TTTCATTCGATGCTTAACAT+AGG - Chr7:77464597-77464616 None:intergenic 30.0%
! ATCAAGTTGTTTATCCAAAC+AGG + Chr7:77463919-77463938 MsG0780040434.01.T01:intron 30.0%
! GAATTGGCAAATTGTTGTTA+AGG + Chr7:77465583-77465602 MsG0780040434.01.T01:three_prime_UTR 30.0%
! TCAAGTTGTTTATCCAAACA+GGG + Chr7:77463920-77463939 MsG0780040434.01.T01:intron 30.0%
! TCATGCTTTTGTACAAATTC+TGG - Chr7:77464837-77464856 None:intergenic 30.0%
! TTGCCTTTCCTTTGAATTTT+AGG - Chr7:77465425-77465444 None:intergenic 30.0%
! TTTCCTTTGAATTTTAGGCA+TGG - Chr7:77465420-77465439 None:intergenic 30.0%
!! CTTTGGGATGATATAAAGAA+AGG + Chr7:77464798-77464817 MsG0780040434.01.T01:CDS 30.0%
!! GAAAAATAGTTGGTGTCAAT+TGG + Chr7:77465559-77465578 MsG0780040434.01.T01:three_prime_UTR 30.0%
!! GCTGCATATGATTTTAAAGA+AGG - Chr7:77464252-77464271 None:intergenic 30.0%
AATACAGTTGCGAACAGTAA+TGG + Chr7:77464631-77464650 MsG0780040434.01.T01:intron 35.0%
AATAGCCATCACCAAAAAGA+TGG - Chr7:77465523-77465542 None:intergenic 35.0%
AATGTTGATCATATTGCCAG+GGG - Chr7:77464179-77464198 None:intergenic 35.0%
ACAAGGTTGAATATTGGTGT+TGG + Chr7:77465722-77465741 MsG0780040434.01.T01:three_prime_UTR 35.0%
ACATGATGAATAGTTCGTGA+AGG + Chr7:77465457-77465476 MsG0780040434.01.T01:three_prime_UTR 35.0%
ACGAACTATTCATCATGTAC+TGG - Chr7:77465455-77465474 None:intergenic 35.0%
AGCGGTTGTTACGATAATTA+TGG + Chr7:77463763-77463782 MsG0780040434.01.T01:intron 35.0%
ATAACACAGAGAGATAGCAA+AGG + Chr7:77463433-77463452 MsG0780040434.01.T01:five_prime_UTR 35.0%
ATAACATGTAGTCACGTACA+GGG - Chr7:77465114-77465133 None:intergenic 35.0%
ATATTCAACCTTGTTCTCCA+AGG - Chr7:77465716-77465735 None:intergenic 35.0%
CATGATGAATAGTTCGTGAA+GGG + Chr7:77465458-77465477 MsG0780040434.01.T01:three_prime_UTR 35.0%
CTCCAAGGAACTGATAAAAA+CGG + Chr7:77463632-77463651 MsG0780040434.01.T01:CDS 35.0%
GTATAAATTATGGGGACGTT+TGG - Chr7:77463857-77463876 None:intergenic 35.0%
GTATTGTGGATAAAGGACAA+GGG + Chr7:77465750-77465769 MsG0780040434.01.T01:three_prime_UTR 35.0%
GTTGGTTAATTAGAGAGGAT+TGG + Chr7:77465797-77465816 MsG0780040434.01.T01:three_prime_UTR 35.0%
GTTGTTAAGGAAGAAATCCT+TGG + Chr7:77465596-77465615 MsG0780040434.01.T01:three_prime_UTR 35.0%
TATTCAGTGAGTTACCAGAT+GGG + Chr7:77464319-77464338 MsG0780040434.01.T01:CDS 35.0%
TCAATTCCATAATCAGTGAG+TGG - Chr7:77464879-77464898 None:intergenic 35.0%
TGGAGAACAAGGTTGAATAT+TGG + Chr7:77465716-77465735 MsG0780040434.01.T01:three_prime_UTR 35.0%
TTACAATTCTTCACTCTCCA+AGG + Chr7:77463617-77463636 MsG0780040434.01.T01:CDS 35.0%
TTATTCAGTGAGTTACCAGA+TGG + Chr7:77464318-77464337 MsG0780040434.01.T01:CDS 35.0%
TTCCAGATGAATTTAAGGAC+AGG + Chr7:77464430-77464449 MsG0780040434.01.T01:CDS 35.0%
TTGCCATGCCTAAAATTCAA+AGG + Chr7:77465414-77465433 MsG0780040434.01.T01:three_prime_UTR 35.0%
! TAAAGATTTTCCCTTCACCA+AGG - Chr7:77465616-77465635 None:intergenic 35.0%
! TATAGCACAGACCATCTTTT+TGG + Chr7:77465509-77465528 MsG0780040434.01.T01:three_prime_UTR 35.0%
! TGGAAAGTATGAAGAGAGAT+GGG + Chr7:77464947-77464966 MsG0780040434.01.T01:CDS 35.0%
! TTGGAAAGTATGAAGAGAGA+TGG + Chr7:77464946-77464965 MsG0780040434.01.T01:CDS 35.0%
!! TCTGTGTTATTTACTGTGCA+GGG - Chr7:77463423-77463442 None:intergenic 35.0%
!!! CTTTTGGAAAGGCATTGAAT+TGG + Chr7:77465645-77465664 MsG0780040434.01.T01:three_prime_UTR 35.0%
AACAGCAAGAAGAAGAGGAT+TGG + Chr7:77463540-77463559 MsG0780040434.01.T01:CDS 40.0%
AAGGAAGAAATCCTTGGTGA+AGG + Chr7:77465602-77465621 MsG0780040434.01.T01:three_prime_UTR 40.0%
ACGAATGAGGATAATTCTCC+TGG + Chr7:77464375-77464394 MsG0780040434.01.T01:CDS 40.0%
AGCCTGTCCTTAAATTCATC+TGG - Chr7:77464435-77464454 None:intergenic 40.0%
AGGAAGAAATCCTTGGTGAA+GGG + Chr7:77465603-77465622 MsG0780040434.01.T01:three_prime_UTR 40.0%
ATCGTTGGAAAACACTATCC+CGG + Chr7:77463671-77463690 MsG0780040434.01.T01:CDS 40.0%
ATTCAAAGGAAAGGCAAGGA+AGG + Chr7:77465428-77465447 MsG0780040434.01.T01:three_prime_UTR 40.0%
CAACTTTCTGATTGCAACCT+TGG + Chr7:77465696-77465715 MsG0780040434.01.T01:three_prime_UTR 40.0%
CAAGGTTGCAATCAGAAAGT+TGG - Chr7:77465698-77465717 None:intergenic 40.0%
CATAACATGTAGTCACGTAC+AGG - Chr7:77465115-77465134 None:intergenic 40.0%
CCAACAATAGATAGCATGCA+CGG - Chr7:77465260-77465279 None:intergenic 40.0%
CGAATGAGGATAATTCTCCT+GGG + Chr7:77464376-77464395 MsG0780040434.01.T01:CDS 40.0%
GGTATTGTGGATAAAGGACA+AGG + Chr7:77465749-77465768 MsG0780040434.01.T01:three_prime_UTR 40.0%
GTCACCTGGTATATAGTTTG+AGG + Chr7:77465328-77465347 MsG0780040434.01.T01:three_prime_UTR 40.0%
GTGAAGGGAAAATCTTTAGC+AGG + Chr7:77465618-77465637 MsG0780040434.01.T01:three_prime_UTR 40.0%
TGCACGTAAACGATGCAAAA+TGG - Chr7:77464345-77464364 None:intergenic 40.0%
TTCAAAGGAAAGGCAAGGAA+GGG + Chr7:77465429-77465448 MsG0780040434.01.T01:three_prime_UTR 40.0%
TTTCACATCATGTGGGGTTA+TGG + Chr7:77465820-77465839 MsG0780040434.01.T01:three_prime_UTR 40.0%
! AATAGGCCACTCACTGATTA+TGG + Chr7:77464870-77464889 MsG0780040434.01.T01:CDS 40.0%
! ACACAGCTTTATACCCAAAG+GGG - Chr7:77465236-77465255 None:intergenic 40.0%
! ATCTTTAGCAGGAGAGCTTT+TGG + Chr7:77465629-77465648 MsG0780040434.01.T01:three_prime_UTR 40.0%
! CACACAGCTTTATACCCAAA+GGG - Chr7:77465237-77465256 None:intergenic 40.0%
! GATAGTGTTTTCCAACGATG+CGG - Chr7:77463670-77463689 None:intergenic 40.0%
! TGTTATTTACTGTGCAGGGT+GGG - Chr7:77463419-77463438 None:intergenic 40.0%
!! ACAGACCATCTTTTTGGTGA+TGG + Chr7:77465515-77465534 MsG0780040434.01.T01:three_prime_UTR 40.0%
!! AGGATTGGTTTCACATCATG+TGG + Chr7:77465812-77465831 MsG0780040434.01.T01:three_prime_UTR 40.0%
!! AGGTTGAATATTGGTGTTGG+AGG + Chr7:77465725-77465744 MsG0780040434.01.T01:three_prime_UTR 40.0%
!! CTCTGTGTTATTTACTGTGC+AGG - Chr7:77463424-77463443 None:intergenic 40.0%
!! GATTGGTTTCACATCATGTG+GGG + Chr7:77465814-77465833 MsG0780040434.01.T01:three_prime_UTR 40.0%
!! GGATTGGTTTCACATCATGT+GGG + Chr7:77465813-77465832 MsG0780040434.01.T01:three_prime_UTR 40.0%
!! GTTGGTGTCAATTGGAGAAT+TGG + Chr7:77465567-77465586 MsG0780040434.01.T01:three_prime_UTR 40.0%
!! TATCATCCCAAAGGTACTGA+AGG - Chr7:77464791-77464810 None:intergenic 40.0%
!! TTGAATATTGGTGTTGGAGG+CGG + Chr7:77465728-77465747 MsG0780040434.01.T01:three_prime_UTR 40.0%
!!! TGCCGTTTTTATCAGTTCCT+TGG - Chr7:77463637-77463656 None:intergenic 40.0%
AAGTATGAAGAGAGATGGGC+AGG + Chr7:77464951-77464970 MsG0780040434.01.T01:CDS 45.0%
ACACAGAGAGATAGCAAAGG+CGG + Chr7:77463436-77463455 MsG0780040434.01.T01:five_prime_UTR 45.0%
ACATCATGTGGGGTTATGGA+AGG + Chr7:77465824-77465843 MsG0780040434.01.T01:three_prime_UTR 45.0%
ACTTTGTACTTCATCCACCC+TGG + Chr7:77464459-77464478 MsG0780040434.01.T01:CDS 45.0%
CAAGAGACCGAGTGTATTGT+TGG - Chr7:77465943-77465962 None:intergenic 45.0%
CATAATTTAGGCCTCTTGGC+TGG + Chr7:77464068-77464087 MsG0780040434.01.T01:intron 45.0%
CCAAACGAGTATGCAACTGA+AGG - Chr7:77464930-77464949 None:intergenic 45.0%
CCATGAACAGCAAGAAGAAG+AGG + Chr7:77463535-77463554 MsG0780040434.01.T01:CDS 45.0%
CCGTGCATGCTATCTATTGT+TGG + Chr7:77465257-77465276 MsG0780040434.01.T01:three_prime_UTR 45.0%
CCTTCAGTTGCATACTCGTT+TGG + Chr7:77464927-77464946 MsG0780040434.01.T01:CDS 45.0%
CTTTGTACTTCATCCACCCT+GGG + Chr7:77464460-77464479 MsG0780040434.01.T01:CDS 45.0%
GAACGCTTCTATATGTCACC+TGG + Chr7:77465314-77465333 MsG0780040434.01.T01:three_prime_UTR 45.0%
GATTGCAACCTTGGAGAACA+AGG + Chr7:77465705-77465724 MsG0780040434.01.T01:three_prime_UTR 45.0%
GTGCATATACTTGCCCCTTT+GGG + Chr7:77465220-77465239 MsG0780040434.01.T01:three_prime_UTR 45.0%
GTTCATGGAGAAGGAAAGGT+CGG - Chr7:77463523-77463542 None:intergenic 45.0%
TGCTGTTCATGGAGAAGGAA+AGG - Chr7:77463527-77463546 None:intergenic 45.0%
TGTGCATATACTTGCCCCTT+TGG + Chr7:77465219-77465238 MsG0780040434.01.T01:three_prime_UTR 45.0%
TTAGGTACGCTGTACACAAG+TGG - Chr7:77465026-77465045 None:intergenic 45.0%
TTCATCGGAGGAATTCTCAG+TGG + Chr7:77463466-77463485 MsG0780040434.01.T01:CDS 45.0%
TTCATGGAGAAGGAAAGGTC+GGG - Chr7:77463522-77463541 None:intergenic 45.0%
! CTTGGCAGGTTTTTCTTGAG+TGG + Chr7:77464510-77464529 MsG0780040434.01.T01:CDS 45.0%
! GCACACAGCTTTATACCCAA+AGG - Chr7:77465238-77465257 None:intergenic 45.0%
! GCAGGTTTTTCTTGAGTGGA+GGG + Chr7:77464514-77464533 MsG0780040434.01.T01:CDS 45.0%
!! CCTCTTCTTCTTGCTGTTCA+TGG - Chr7:77463538-77463557 None:intergenic 45.0%
!! CTTCTTGCTGTTCATGGAGA+AGG - Chr7:77463532-77463551 None:intergenic 45.0%
!! GTGTTATTTACTGTGCAGGG+TGG - Chr7:77463420-77463439 None:intergenic 45.0%
!! TAGCAGGAGAGCTTTTGGAA+AGG + Chr7:77465634-77465653 MsG0780040434.01.T01:three_prime_UTR 45.0%
AAAGGCGGCGATGAGTTCAT+CGG + Chr7:77463451-77463470 MsG0780040434.01.T01:exon 50.0%
AAGAGTAGCCATGACAAGCC+TGG - Chr7:77464493-77464512 None:intergenic 50.0%
AAGCCGCCTTCAGTACCTTT+GGG + Chr7:77464782-77464801 MsG0780040434.01.T01:CDS 50.0%
ACGATGCAAAATGGCCCATC+TGG - Chr7:77464336-77464355 None:intergenic 50.0%
AGATCGGAGAAGTCTTCGTC+GGG - Chr7:77463590-77463609 None:intergenic 50.0%
AGGCATCCCAGTCAAGTGAA+AGG - Chr7:77464910-77464929 None:intergenic 50.0%
AGGCTTGTCATGGCTACTCT+TGG + Chr7:77464492-77464511 MsG0780040434.01.T01:CDS 50.0%
AGTACATAACGCCAGCCAAG+AGG - Chr7:77464082-77464101 None:intergenic 50.0%
ATCGGAGGAATTCTCAGTGG+TGG + Chr7:77463469-77463488 MsG0780040434.01.T01:CDS 50.0%
ATGAAGAGAGATGGGCAGGA+AGG + Chr7:77464955-77464974 MsG0780040434.01.T01:CDS 50.0%
CAACAGTTGTGAGTGCAGAG+CGG + Chr7:77464271-77464290 MsG0780040434.01.T01:CDS 50.0%
CACCTCAAGATTGTCATCCC+AGG - Chr7:77464396-77464415 None:intergenic 50.0%
CTAGCTAGCATACGTTGCAG+TGG - Chr7:77463706-77463725 None:intergenic 50.0%
CTCCTGGGATGACAATCTTG+AGG + Chr7:77464391-77464410 MsG0780040434.01.T01:CDS 50.0%
CTGGGATGACAATCTTGAGG+TGG + Chr7:77464394-77464413 MsG0780040434.01.T01:CDS 50.0%
GAGATCGGAGAAGTCTTCGT+CGG - Chr7:77463591-77463610 None:intergenic 50.0%
GCACAGCACTGTTACGAATG+AGG + Chr7:77464362-77464381 MsG0780040434.01.T01:CDS 50.0%
GCATCCCAGTCAAGTGAAAG+GGG - Chr7:77464908-77464927 None:intergenic 50.0%
GGAGGCGGTATTGTGGATAA+AGG + Chr7:77465743-77465762 MsG0780040434.01.T01:three_prime_UTR 50.0%
GGCATCCCAGTCAAGTGAAA+GGG - Chr7:77464909-77464928 None:intergenic 50.0%
TAAGCCGCCTTCAGTACCTT+TGG + Chr7:77464781-77464800 MsG0780040434.01.T01:CDS 50.0%
TGAAGAGAGATGGGCAGGAA+GGG + Chr7:77464956-77464975 MsG0780040434.01.T01:CDS 50.0%
TTGTCATGGCTACTCTTGGC+AGG + Chr7:77464496-77464515 MsG0780040434.01.T01:CDS 50.0%
! GGCAGGTTTTTCTTGAGTGG+AGG + Chr7:77464513-77464532 MsG0780040434.01.T01:CDS 50.0%
! GTTGCAGTGGAGGAAAGTAC+CGG - Chr7:77463693-77463712 None:intergenic 50.0%
!! CATCCCAAAGGTACTGAAGG+CGG - Chr7:77464788-77464807 None:intergenic 50.0%
!! TTGCAGTGGAGGAAAGTACC+GGG - Chr7:77463692-77463711 None:intergenic 50.0%
ACTCGCATTCTCCGCATCGT+TGG + Chr7:77463656-77463675 MsG0780040434.01.T01:CDS 55.0%
AGAAGTCTTCGTCGGGAGAG+AGG - Chr7:77463583-77463602 None:intergenic 55.0%
CCAGTCAAGTGAAAGGGGTC+GGG - Chr7:77464903-77464922 None:intergenic 55.0%
CCCAGTCAAGTGAAAGGGGT+CGG - Chr7:77464904-77464923 None:intergenic 55.0%
CCGACCCCTTTCACTTGACT+GGG + Chr7:77464901-77464920 MsG0780040434.01.T01:CDS 55.0%
CTCAGTGGTGGTCCTAGCAT+CGG + Chr7:77463481-77463500 MsG0780040434.01.T01:CDS 55.0%
GCTAGCATACGTTGCAGTGG+AGG - Chr7:77463703-77463722 None:intergenic 55.0%
GCTTCGTTCCAGGCTTGTCA+TGG + Chr7:77464482-77464501 MsG0780040434.01.T01:CDS 55.0%
! TGGTGTTGGAGGCGGTATTG+TGG + Chr7:77465736-77465755 MsG0780040434.01.T01:three_prime_UTR 55.0%
!! AAGGAAAGGTCGGGCATCGA+CGG - Chr7:77463513-77463532 None:intergenic 55.0%
ACAAGCCTGGAACGAAGCCC+AGG - Chr7:77464480-77464499 None:intergenic 60.0%
CAAGCCTGGAACGAAGCCCA+GGG - Chr7:77464479-77464498 None:intergenic 60.0%
CCCGACCCCTTTCACTTGAC+TGG + Chr7:77464900-77464919 MsG0780040434.01.T01:CDS 60.0%
TGAGTGCAGAGCGGCTGAAG+AGG + Chr7:77464280-77464299 MsG0780040434.01.T01:CDS 60.0%
CGACGGCGAGATCCGATGCT+AGG - Chr7:77463496-77463515 None:intergenic 65.0%
GGCGGCGATGAGTTCATCGG+AGG + Chr7:77463454-77463473 MsG0780040434.01.T01:exon 65.0%
TCCACCCTGGGCTTCGTTCC+AGG + Chr7:77464472-77464491 MsG0780040434.01.T01:CDS 65.0%
GCCTGGAACGAAGCCCAGGG+TGG - Chr7:77464476-77464495 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 77463413 77466110 77463413 ID=MsG0780040434.01;Name=MsG0780040434.01
Chr7 mRNA 77463413 77466110 77463413 ID=MsG0780040434.01.T01;Parent=MsG0780040434.01;Name=MsG0780040434.01.T01;_AED=0.20;_eAED=0.20;_QI=48|1|1|1|1|1|3|1103|248
Chr7 exon 77463413 77463692 77463413 ID=MsG0780040434.01.T01:exon:14865;Parent=MsG0780040434.01.T01
Chr7 exon 77464271 77464535 77464271 ID=MsG0780040434.01.T01:exon:14866;Parent=MsG0780040434.01.T01
Chr7 exon 77464758 77466110 77464758 ID=MsG0780040434.01.T01:exon:14867;Parent=MsG0780040434.01.T01
Chr7 five_prime_UTR 77463413 77463460 77463413 ID=MsG0780040434.01.T01:five_prime_utr;Parent=MsG0780040434.01.T01
Chr7 CDS 77463461 77463692 77463461 ID=MsG0780040434.01.T01:cds;Parent=MsG0780040434.01.T01
Chr7 CDS 77464271 77464535 77464271 ID=MsG0780040434.01.T01:cds;Parent=MsG0780040434.01.T01
Chr7 CDS 77464758 77465007 77464758 ID=MsG0780040434.01.T01:cds;Parent=MsG0780040434.01.T01
Chr7 three_prime_UTR 77465008 77466110 77465008 ID=MsG0780040434.01.T01:three_prime_utr;Parent=MsG0780040434.01.T01
Gene Sequence

>MsG0780040434.01.T01

ATGAGTTCATCGGAGGAATTCTCAGTGGTGGTCCTAGCATCGGATCTCGCCGTCGATGCCCGACCTTTCCTTCTCCATGAACAGCAAGAAGAAGAGGATTGGCACGATTGTTCTCAGTACCTCTCTCCCGACGAAGACTTCTCCGATCTCGAATTCTTACAATTCTTCACTCTCCAAGGAACTGATAAAAACGGCACTCGCATTCTCCGCATCGTTGGAAAACACTATCCCGCAACAGTTGTGAGTGCAGAGCGGCTGAAGAGGTATGTATTTCACAAATTATTCAGTGAGTTACCAGATGGGCCATTTTGCATCGTTTACGTGCACAGCACTGTTACGAATGAGGATAATTCTCCTGGGATGACAATCTTGAGGTGGATTTATGAAGATCTTCCAGATGAATTTAAGGACAGGCTTCAAACTTTGTACTTCATCCACCCTGGGCTTCGTTCCAGGCTTGTCATGGCTACTCTTGGCAGGTTTTTCTTGAGTGGAGGATTATATTGGAAGATCAAGTATGTAAGCCGCCTTCAGTACCTTTGGGATGATATAAAGAAAGGAGAGATTGAGATACCAGAATTTGTACAAAAGCATGATGATATTTTGGAGAATAGGCCACTCACTGATTATGGAATTGAACCCGACCCCTTTCACTTGACTGGGATGCCTTCAGTTGCATACTCGTTTGGAAAGTATGAAGAGAGATGGGCAGGAAGGGATCATATCTCTGAGTATAAATATCACTGA
Protein sequence

>MsG0780040434.01.T01

MSSSEEFSVVVLASDLAVDARPFLLHEQQEEEDWHDCSQYLSPDEDFSDLEFLQFFTLQGTDKNGTRILRIVGKHYPATVVSAERLKRYVFHKLFSELPDGPFCIVYVHSTVTNEDNSPGMTILRWIYEDLPDEFKDRLQTLYFIHPGLRSRLVMATLGRFFLSGGLYWKIKYVSRLQYLWDDIKKGEIEIPEFVQKHDDILENRPLTDYGIEPDPFHLTGMPSVAYSFGKYEERWAGRDHISEYKYH*