Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022667.01.T01 | XP_013457196.1 | 100 | 147 | 0 | 0 | 1 | 147 | 1 | 147 | 8.79E-98 | 288 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022667.01.T01 | A0A072UNE2 | 100.000 | 147 | 0 | 0 | 1 | 147 | 1 | 147 | 4.20e-98 | 288 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000218.01 | MsG0480022667.01 | 0.814108 | 1.822271e-51 | 1.211804e-48 |
| MsG0280010596.01 | MsG0480022667.01 | 0.828810 | 7.310813e-55 | 7.345122e-52 |
| MsG0480020935.01 | MsG0480022667.01 | 0.821059 | 4.937874e-53 | 3.975526e-50 |
| MsG0480022667.01 | MsG0480023928.01 | 0.832258 | 1.047480e-55 | 1.164413e-52 |
| MsG0480022667.01 | MsG0580024790.01 | 0.809437 | 1.893469e-50 | 1.111664e-47 |
| MsG0480022667.01 | MsG0580025407.01 | 0.815668 | 8.214661e-52 | 5.699587e-49 |
| MsG0480022667.01 | MsG0580028232.01 | 0.807385 | 5.187253e-50 | 2.885248e-47 |
| MsG0480022667.01 | MsG0780040434.01 | 0.830944 | 2.207164e-55 | 2.360376e-52 |
| MsG0480022667.01 | MsG0880047055.01 | 0.806088 | 9.749261e-50 | 5.244558e-47 |
| MsG0480022667.01 | MsG0880047584.01 | 0.812097 | 5.033116e-51 | 3.170761e-48 |
| MsG0280006711.01 | MsG0480022667.01 | 0.829740 | 4.345946e-55 | 4.487970e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022667.01.T01 | MTR_4g094242 | 100.000 | 147 | 0 | 0 | 1 | 147 | 1 | 147 | 1.07e-101 | 288 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022667.01.T01 | AT5G64180 | 79.286 | 140 | 29 | 0 | 8 | 147 | 10 | 149 | 1.43e-69 | 207 |
| MsG0480022667.01.T01 | AT5G64180 | 79.286 | 140 | 29 | 0 | 8 | 147 | 10 | 149 | 1.43e-69 | 207 |
Find 29 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTTCGGCTTGTCGTTTCT+CGG | 0.286319 | 4:+75309647 | None:intergenic |
| AGCGCGAGCGGCGGCGGTGA+TGG | 0.397256 | 4:+75309674 | None:intergenic |
| GGAACGAGCGCGAGCGGCGG+CGG | 0.415630 | 4:+75309668 | None:intergenic |
| GTGTAGAGTCTGCTACTAGA+AGG | 0.442589 | 4:+75310173 | None:intergenic |
| GGCGTCGAGTTCTCGTTCTA+AGG | 0.445222 | 4:+75309698 | None:intergenic |
| TTTGATAGTGCGGGCTTGAT+CGG | 0.446495 | 4:+75309743 | None:intergenic |
| AGCGGCTTTCTGTGACGCTT+CGG | 0.485423 | 4:+75309632 | None:intergenic |
| GGCTTGATCGGCGGCGAGAG+AGG | 0.490699 | 4:+75309755 | None:intergenic |
| AAGTCGTTGAGAGATGGATT+CGG | 0.498251 | 4:-75309904 | MsG0480022667.01.T01:exon |
| GTGAATCATGAGATTCGAAA+AGG | 0.499244 | 4:+75310222 | None:intergenic |
| ATCGAGGAGTTTGATAGTGC+GGG | 0.500242 | 4:+75309734 | None:intergenic |
| CTCGGAACGAGCGCGAGCGG+CGG | 0.505434 | 4:+75309665 | None:intergenic |
| GCGAGCGGCGGCGGTGATGG+CGG | 0.515286 | 4:+75309677 | None:intergenic |
| GTCGTCGACGAAAACAGCTT+TGG | 0.521805 | 4:+75309794 | None:intergenic |
| CGGATTCGAAAGGAGAAAGA+TGG | 0.528818 | 4:-75309884 | MsG0480022667.01.T01:exon |
| AGAAGGTTCGATAGGTTTGA+CGG | 0.547880 | 4:+75310190 | None:intergenic |
| GAGATGAAGTCGTTGAGAGA+TGG | 0.551499 | 4:-75309910 | MsG0480022667.01.T01:exon |
| GCAGACTCTACACGAAAACT+AGG | 0.566496 | 4:-75310163 | MsG0480022667.01.T01:exon |
| CGAGTTCTCGTTCTAAGGTG+TGG | 0.570651 | 4:+75309703 | None:intergenic |
| TTTCTCGGAACGAGCGCGAG+CGG | 0.570899 | 4:+75309662 | None:intergenic |
| CTGCTACTAGAAGGTTCGAT+AGG | 0.575152 | 4:+75310182 | None:intergenic |
| CGATAGGTTTGACGGCGAAG+AGG | 0.576634 | 4:+75310198 | None:intergenic |
| AGGTGTGGACGCGTTGATCG+AGG | 0.581765 | 4:+75309718 | None:intergenic |
| ATCTCTCAACGACTTCATCT+CGG | 0.586816 | 4:+75309912 | None:intergenic |
| TTCAAGAGCGCGTAATTCAG+CGG | 0.601000 | 4:+75309614 | None:intergenic |
| GATAGTGCGGGCTTGATCGG+CGG | 0.606803 | 4:+75309746 | None:intergenic |
| GATCGAGGAGTTTGATAGTG+CGG | 0.631084 | 4:+75309733 | None:intergenic |
| TCGAAAGGAGAAAGATGGAG+CGG | 0.658226 | 4:-75309879 | MsG0480022667.01.T01:exon |
| GAGATGGATTCGGATTCGAA+AGG | 0.675806 | 4:-75309894 | MsG0480022667.01.T01:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTGTACAAAAATTATTTTC+AGG | - | Chr4:75309496-75309515 | MsG0480022667.01.T01:intron | 20.0% |
| !!! | CTGTACAAAAATTATTTTCA+GGG | - | Chr4:75309497-75309516 | MsG0480022667.01.T01:intron | 20.0% |
| !!! | GGTTTCTGATTTTTTTAATA+CGG | - | Chr4:75310028-75310047 | MsG0480022667.01.T01:exon | 20.0% |
| !!! | TGATAATGATATTAAGCTTT+TGG | - | Chr4:75310112-75310131 | MsG0480022667.01.T01:exon | 20.0% |
| ! | CAATAACTCAATTCGAAATT+AGG | + | Chr4:75309977-75309996 | None:intergenic | 25.0% |
| ! | TTGGAGAAGTTAATAATTGA+TGG | - | Chr4:75310007-75310026 | MsG0480022667.01.T01:exon | 25.0% |
| !!! | TGATTTTTTTAATACGGTAG+AGG | - | Chr4:75310034-75310053 | MsG0480022667.01.T01:exon | 25.0% |
| GGATTAATAGTACTTACTTG+TGG | + | Chr4:75309956-75309975 | None:intergenic | 30.0% | |
| CGAAAGAAACTAAGAACAAC+AGG | + | Chr4:75310201-75310220 | None:intergenic | 35.0% | |
| GAGTTATTGTGATCTGTGAT+TGG | - | Chr4:75309988-75310007 | MsG0480022667.01.T01:exon | 35.0% | |
| GTGAATCATGAGATTCGAAA+AGG | + | Chr4:75309303-75309322 | None:intergenic | 35.0% | |
| ! | CAACAGGTTTAGTGTTTTAC+AGG | + | Chr4:75310185-75310204 | None:intergenic | 35.0% |
| !! | AGAGGTGTTTGAGTTACATA+TGG | - | Chr4:75310052-75310071 | MsG0480022667.01.T01:exon | 35.0% |
| AGAAGGTTCGATAGGTTTGA+CGG | + | Chr4:75309335-75309354 | None:intergenic | 40.0% | |
| ATCTCTCAACGACTTCATCT+CGG | + | Chr4:75309613-75309632 | None:intergenic | 40.0% | |
| GAAGCTATTATCCAAACGCT+TGG | - | Chr4:75310134-75310153 | MsG0480022667.01.T01:exon | 40.0% | |
| ! | AAGTCGTTGAGAGATGGATT+CGG | - | Chr4:75309618-75309637 | MsG0480022667.01.T01:exon | 40.0% |
| ! | ATTCTTTTCCACCAAGCGTT+TGG | + | Chr4:75310148-75310167 | None:intergenic | 40.0% |
| !!! | TGTGGTGTTTTCGAGTTCTT+TGG | + | Chr4:75309938-75309957 | None:intergenic | 40.0% |
| CGGATTCGAAAGGAGAAAGA+TGG | - | Chr4:75309638-75309657 | MsG0480022667.01.T01:exon | 45.0% | |
| CTGCTACTAGAAGGTTCGAT+AGG | + | Chr4:75309343-75309362 | None:intergenic | 45.0% | |
| GAGATGAAGTCGTTGAGAGA+TGG | - | Chr4:75309612-75309631 | MsG0480022667.01.T01:exon | 45.0% | |
| GCAGACTCTACACGAAAACT+AGG | - | Chr4:75309359-75309378 | MsG0480022667.01.T01:exon | 45.0% | |
| GCTATTATCCAAACGCTTGG+TGG | - | Chr4:75310137-75310156 | MsG0480022667.01.T01:exon | 45.0% | |
| GTGTAGAGTCTGCTACTAGA+AGG | + | Chr4:75309352-75309371 | None:intergenic | 45.0% | |
| TCGAAAGGAGAAAGATGGAG+CGG | - | Chr4:75309643-75309662 | MsG0480022667.01.T01:exon | 45.0% | |
| ! | GAGATGGATTCGGATTCGAA+AGG | - | Chr4:75309628-75309647 | MsG0480022667.01.T01:exon | 45.0% |
| ! | TTCAAGAGCGCGTAATTCAG+CGG | + | Chr4:75309911-75309930 | None:intergenic | 45.0% |
| !! | ATCGAGGAGTTTGATAGTGC+GGG | + | Chr4:75309791-75309810 | None:intergenic | 45.0% |
| !! | GATCGAGGAGTTTGATAGTG+CGG | + | Chr4:75309792-75309811 | None:intergenic | 45.0% |
| !! | TTTGATAGTGCGGGCTTGAT+CGG | + | Chr4:75309782-75309801 | None:intergenic | 45.0% |
| GTCGTCGACGAAAACAGCTT+TGG | + | Chr4:75309731-75309750 | None:intergenic | 50.0% | |
| !! | CGAGTTCTCGTTCTAAGGTG+TGG | + | Chr4:75309822-75309841 | None:intergenic | 50.0% |
| AGCGGCTTTCTGTGACGCTT+CGG | + | Chr4:75309893-75309912 | None:intergenic | 55.0% | |
| CGATAGGTTTGACGGCGAAG+AGG | + | Chr4:75309327-75309346 | None:intergenic | 55.0% | |
| ! | CGCTTCGGCTTGTCGTTTCT+CGG | + | Chr4:75309878-75309897 | None:intergenic | 55.0% |
| ! | GGCGTCGAGTTCTCGTTCTA+AGG | + | Chr4:75309827-75309846 | None:intergenic | 55.0% |
| GATAGTGCGGGCTTGATCGG+CGG | + | Chr4:75309779-75309798 | None:intergenic | 60.0% | |
| TTTCTCGGAACGAGCGCGAG+CGG | + | Chr4:75309863-75309882 | None:intergenic | 60.0% | |
| ! | AGGTGTGGACGCGTTGATCG+AGG | + | Chr4:75309807-75309826 | None:intergenic | 60.0% |
| GGCTTGATCGGCGGCGAGAG+AGG | + | Chr4:75309770-75309789 | None:intergenic | 70.0% | |
| CTCGGAACGAGCGCGAGCGG+CGG | + | Chr4:75309860-75309879 | None:intergenic | 75.0% | |
| ! | AGCGCGAGCGGCGGCGGTGA+TGG | + | Chr4:75309851-75309870 | None:intergenic | 80.0% |
| ! | GGAACGAGCGCGAGCGGCGG+CGG | + | Chr4:75309857-75309876 | None:intergenic | 80.0% |
| !! | GCGAGCGGCGGCGGTGATGG+CGG | + | Chr4:75309848-75309867 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 75309268 | 75310276 | 75309268 | ID=MsG0480022667.01; |
| Chr4 | mRNA | 75309268 | 75310276 | 75309268 | ID=MsG0480022667.01.T01;Parent=MsG0480022667.01 |
| Chr4 | exon | 75309268 | 75309490 | 75309268 | ID=MsG0480022667.01.T01.exon;Parent=MsG0480022667.01.T01 |
| Chr4 | CDS | 75309375 | 75309490 | 75309375 | ID=MsG0480022667.01.T01.cds;Parent=MsG0480022667.01.T01 |
| Chr4 | CDS | 75309586 | 75309913 | 75309586 | ID=MsG0480022667.01.T01.cds;Parent=MsG0480022667.01.T01 |
| Chr4 | exon | 75309586 | 75310276 | 75309586 | ID=MsG0480022667.01.T01.exon;Parent=MsG0480022667.01.T01 |
| Gene Sequence |
| Protein sequence |
