Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780040486.01 | MsG0880047049.01 | 0.804318 | 2.287990e-49 | 1.176042e-46 |
| MsG0780040486.01 | MsG0880047492.01 | 0.806571 | 7.709925e-50 | 4.198980e-47 |
| MsG0480023279.01 | MsG0780040486.01 | 0.824573 | 7.503052e-54 | 6.672179e-51 |
| MsG0480023418.01 | MsG0780040486.01 | 0.823648 | 1.236741e-53 | 1.071304e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCTTCGTTGGTAGATTT+TGG | 0.146979 | 7:-78306677 | MsG0780040486.01.T01:CDS |
| GATGGTGATGGTGGGGTGCT+TGG | 0.249664 | 7:-78306929 | MsG0780040486.01.T01:CDS |
| ATGGTGATGGTGGGGTGCTT+GGG | 0.268747 | 7:-78306928 | MsG0780040486.01.T01:CDS |
| GGTTGTTGTGGGTGGGTTTG+TGG | 0.311520 | 7:-78306982 | MsG0780040486.01.T01:CDS |
| GGGTGCTTGGGAGTGTGGAG+AGG | 0.329723 | 7:-78306916 | MsG0780040486.01.T01:CDS |
| TGGTGGGGTGCTTGGGAGTG+TGG | 0.366470 | 7:-78306921 | MsG0780040486.01.T01:CDS |
| GAATCGTGGGTGATGTTTCT+TGG | 0.376584 | 7:-78306637 | MsG0780040486.01.T01:CDS |
| GGCGAAATGGTGGTTGTTGT+GGG | 0.384663 | 7:-78306993 | None:intergenic |
| TGGAGAGGGTGAAGAGGAGG+AGG | 0.385232 | 7:-78306901 | MsG0780040486.01.T01:CDS |
| AAATGGTGGTTGTTGTGGGT+GGG | 0.390087 | 7:-78306989 | None:intergenic |
| GGGCGAAATGGTGGTTGTTG+TGG | 0.409462 | 7:-78306994 | None:intergenic |
| GGTGCTTGGGAGTGTGGAGA+GGG | 0.420858 | 7:-78306915 | MsG0780040486.01.T01:CDS |
| TGAGAGTGTGGATTGTGCTT+TGG | 0.423013 | 7:-78306852 | MsG0780040486.01.T01:CDS |
| GAAATGGTGGTTGTTGTGGG+TGG | 0.428250 | 7:-78306990 | None:intergenic |
| GAAGGAAACGACGTCGTAAA+GGG | 0.435671 | 7:-78306952 | MsG0780040486.01.T01:CDS |
| GTGTGGAGAGGGTGAAGAGG+AGG | 0.442714 | 7:-78306904 | MsG0780040486.01.T01:CDS |
| TGGTGAGAAAGAAGAGGAAG+AGG | 0.445099 | 7:-78306798 | MsG0780040486.01.T01:CDS |
| TGGGTTTGTGGTGGTTGAGA+AGG | 0.461400 | 7:-78306970 | MsG0780040486.01.T01:CDS |
| GGAGTGTGGAGAGGGTGAAG+AGG | 0.463225 | 7:-78306907 | MsG0780040486.01.T01:CDS |
| TGGATGATGATTTGTCTTGT+GGG | 0.467556 | 7:-78306748 | MsG0780040486.01.T01:CDS |
| GTGATGTTTCTTGGTTGTGA+TGG | 0.472074 | 7:-78306628 | None:intergenic |
| TGTTGTGGGTGGGTTTGTGG+TGG | 0.472850 | 7:-78306979 | MsG0780040486.01.T01:CDS |
| AGAAGGAAACGACGTCGTAA+AGG | 0.480321 | 7:-78306953 | MsG0780040486.01.T01:CDS |
| CGTAAAGGGGATGGTGATGG+TGG | 0.483370 | 7:-78306938 | MsG0780040486.01.T01:CDS |
| GTGGATGATGATTTGTCTTG+TGG | 0.486396 | 7:-78306749 | MsG0780040486.01.T01:CDS |
| GTTGGTGCTGTTATCTTCGT+TGG | 0.494855 | 7:-78306687 | MsG0780040486.01.T01:CDS |
| TGAATTTAGTTTAGTTGTTG+TGG | 0.501174 | 7:-78306771 | MsG0780040486.01.T01:CDS |
| GTAAAGGGGATGGTGATGGT+GGG | 0.520099 | 7:-78306937 | MsG0780040486.01.T01:CDS |
| AGAGGAGGAGGGTAGTGAAG+AGG | 0.545270 | 7:-78306889 | MsG0780040486.01.T01:CDS |
| CGATGGTGGTGAGAAAGAAG+AGG | 0.551023 | 7:-78306804 | MsG0780040486.01.T01:CDS |
| GGAGAGGGTGAAGAGGAGGA+GGG | 0.556307 | 7:-78306900 | MsG0780040486.01.T01:CDS |
| ATTTAGTTTAGTTGTTGTGG+TGG | 0.557221 | 7:-78306768 | MsG0780040486.01.T01:CDS |
| GAGAAAGAAGAGGAAGAGGA+AGG | 0.557528 | 7:-78306794 | MsG0780040486.01.T01:CDS |
| GTCATTGTTGAGAGAATCGT+GGG | 0.564935 | 7:-78306650 | MsG0780040486.01.T01:CDS |
| AAGTGCACAGATTGAGAGTG+TGG | 0.576741 | 7:-78306864 | MsG0780040486.01.T01:CDS |
| CGTCGTAAAGGGGATGGTGA+TGG | 0.596550 | 7:-78306941 | MsG0780040486.01.T01:CDS |
| AAGGAAACGACGTCGTAAAG+GGG | 0.604869 | 7:-78306951 | MsG0780040486.01.T01:CDS |
| TAAAGGGGATGGTGATGGTG+GGG | 0.606361 | 7:-78306936 | MsG0780040486.01.T01:CDS |
| TGTCATTGTTGAGAGAATCG+TGG | 0.617070 | 7:-78306651 | MsG0780040486.01.T01:CDS |
| AAACGACGTCGTAAAGGGGA+TGG | 0.641815 | 7:-78306947 | MsG0780040486.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTTAGTTTAGTTGTTG+TGG | - | Chr7:78306840-78306859 | MsG0780040486.01.T01:CDS | 25.0% |
| ! | ATTTAGTTTAGTTGTTGTGG+TGG | - | Chr7:78306843-78306862 | MsG0780040486.01.T01:CDS | 30.0% |
| !! | TTATCTTCGTTGGTAGATTT+TGG | - | Chr7:78306934-78306953 | MsG0780040486.01.T01:CDS | 30.0% |
| ! | CATGTTGTTGTTGTTGTTGT+TGG | - | Chr7:78306906-78306925 | MsG0780040486.01.T01:CDS | 35.0% |
| ! | TGGATGATGATTTGTCTTGT+GGG | - | Chr7:78306863-78306882 | MsG0780040486.01.T01:CDS | 35.0% |
| !!! | AGAGATTTTGATGATGACGA+TGG | - | Chr7:78306790-78306809 | MsG0780040486.01.T01:CDS | 35.0% |
| GTCATTGTTGAGAGAATCGT+GGG | - | Chr7:78306961-78306980 | MsG0780040486.01.T01:CDS | 40.0% | |
| TGTCATTGTTGAGAGAATCG+TGG | - | Chr7:78306960-78306979 | MsG0780040486.01.T01:CDS | 40.0% | |
| ! | GTGGATGATGATTTGTCTTG+TGG | - | Chr7:78306862-78306881 | MsG0780040486.01.T01:CDS | 40.0% |
| !!! | GATTTTGATGATGACGATGG+TGG | - | Chr7:78306793-78306812 | MsG0780040486.01.T01:CDS | 40.0% |
| AAGGAAACGACGTCGTAAAG+GGG | - | Chr7:78306660-78306679 | MsG0780040486.01.T01:CDS | 45.0% | |
| AAGTGCACAGATTGAGAGTG+TGG | - | Chr7:78306747-78306766 | MsG0780040486.01.T01:CDS | 45.0% | |
| AGAAGGAAACGACGTCGTAA+AGG | - | Chr7:78306658-78306677 | MsG0780040486.01.T01:CDS | 45.0% | |
| GAAGGAAACGACGTCGTAAA+GGG | - | Chr7:78306659-78306678 | MsG0780040486.01.T01:CDS | 45.0% | |
| GAATCGTGGGTGATGTTTCT+TGG | - | Chr7:78306974-78306993 | MsG0780040486.01.T01:CDS | 45.0% | |
| GAGAAAGAAGAGGAAGAGGA+AGG | - | Chr7:78306817-78306836 | MsG0780040486.01.T01:CDS | 45.0% | |
| TGGTGAGAAAGAAGAGGAAG+AGG | - | Chr7:78306813-78306832 | MsG0780040486.01.T01:CDS | 45.0% | |
| ! | TGAGAGTGTGGATTGTGCTT+TGG | - | Chr7:78306759-78306778 | MsG0780040486.01.T01:CDS | 45.0% |
| !! | GTTGGTGCTGTTATCTTCGT+TGG | - | Chr7:78306924-78306943 | MsG0780040486.01.T01:CDS | 45.0% |
| AAACGACGTCGTAAAGGGGA+TGG | - | Chr7:78306664-78306683 | MsG0780040486.01.T01:CDS | 50.0% | |
| TGGGTTTGTGGTGGTTGAGA+AGG | - | Chr7:78306641-78306660 | MsG0780040486.01.T01:CDS | 50.0% | |
| ! | CGATGGTGGTGAGAAAGAAG+AGG | - | Chr7:78306807-78306826 | MsG0780040486.01.T01:CDS | 50.0% |
| !! | GTAAAGGGGATGGTGATGGT+GGG | - | Chr7:78306674-78306693 | MsG0780040486.01.T01:CDS | 50.0% |
| !! | TAAAGGGGATGGTGATGGTG+GGG | - | Chr7:78306675-78306694 | MsG0780040486.01.T01:CDS | 50.0% |
| AGAGGAGGAGGGTAGTGAAG+AGG | - | Chr7:78306722-78306741 | MsG0780040486.01.T01:CDS | 55.0% | |
| CGTCGTAAAGGGGATGGTGA+TGG | - | Chr7:78306670-78306689 | MsG0780040486.01.T01:CDS | 55.0% | |
| ! | TGTTGTGGGTGGGTTTGTGG+TGG | - | Chr7:78306632-78306651 | MsG0780040486.01.T01:CDS | 55.0% |
| !! | ATGGTGATGGTGGGGTGCTT+GGG | - | Chr7:78306683-78306702 | MsG0780040486.01.T01:CDS | 55.0% |
| !! | CGTAAAGGGGATGGTGATGG+TGG | - | Chr7:78306673-78306692 | MsG0780040486.01.T01:CDS | 55.0% |
| GGAGAGGGTGAAGAGGAGGA+GGG | - | Chr7:78306711-78306730 | MsG0780040486.01.T01:CDS | 60.0% | |
| GGAGTGTGGAGAGGGTGAAG+AGG | - | Chr7:78306704-78306723 | MsG0780040486.01.T01:CDS | 60.0% | |
| GTGTGGAGAGGGTGAAGAGG+AGG | - | Chr7:78306707-78306726 | MsG0780040486.01.T01:CDS | 60.0% | |
| TGGAGAGGGTGAAGAGGAGG+AGG | - | Chr7:78306710-78306729 | MsG0780040486.01.T01:CDS | 60.0% | |
| ! | GGTGCTTGGGAGTGTGGAGA+GGG | - | Chr7:78306696-78306715 | MsG0780040486.01.T01:CDS | 60.0% |
| !! | GATGGTGATGGTGGGGTGCT+TGG | - | Chr7:78306682-78306701 | MsG0780040486.01.T01:CDS | 60.0% |
| ! | GGGTGCTTGGGAGTGTGGAG+AGG | - | Chr7:78306695-78306714 | MsG0780040486.01.T01:CDS | 65.0% |
| ! | TGGTGGGGTGCTTGGGAGTG+TGG | - | Chr7:78306690-78306709 | MsG0780040486.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 78306631 | 78307002 | 78306631 | ID=MsG0780040486.01;Name=MsG0780040486.01 |
| Chr7 | mRNA | 78306631 | 78307002 | 78306631 | ID=MsG0780040486.01.T01;Parent=MsG0780040486.01;Name=MsG0780040486.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|123 |
| Chr7 | exon | 78306631 | 78307002 | 78306631 | ID=MsG0780040486.01.T01:exon:16347;Parent=MsG0780040486.01.T01 |
| Chr7 | CDS | 78306631 | 78307002 | 78306631 | ID=MsG0780040486.01.T01:cds;Parent=MsG0780040486.01.T01 |
| Gene Sequence |
| Protein sequence |
