AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047049.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047049.01.T01 MTR_4g119880 97.468 158 3 1 73 230 41 197 1.79e-105 317
MsG0880047049.01.T01 MTR_4g119880 88.235 102 12 0 251 352 353 454 1.46e-56 191
MsG0880047049.01.T01 MTR_4g119880 91.176 34 3 0 231 264 257 290 3.04e-14 73.9
MsG0880047049.01.T01 MTR_7g108675 64.118 170 48 3 73 230 41 209 5.92e-62 205
MsG0880047049.01.T01 MTR_7g108675 75.490 102 25 0 251 352 365 466 5.92e-50 174
MsG0880047049.01.T01 MTR_7g108675 91.176 34 3 0 231 264 269 302 2.54e-14 74.3
MsG0880047049.01.T01 MTR_8g058553 64.118 170 48 3 73 230 41 209 2.59e-61 205
MsG0880047049.01.T01 MTR_8g058553 70.000 50 15 0 251 300 365 414 5.12e-18 85.5
MsG0880047049.01.T01 MTR_8g058553 91.176 34 3 0 231 264 269 302 2.30e-14 74.3
MsG0880047049.01.T01 MTR_3g037340 54.887 133 42 2 98 230 84 198 2.14e-39 145
MsG0880047049.01.T01 MTR_3g037340 53.409 88 41 0 251 338 354 441 1.69e-29 118
MsG0880047049.01.T01 MTR_3g037340 64.583 48 14 1 219 263 243 290 1.62e-12 68.6
MsG0880047049.01.T01 MTR_4g108940 71.429 91 25 1 140 230 104 193 2.95e-39 144
MsG0880047049.01.T01 MTR_4g108940 49.485 97 49 0 251 347 349 445 8.28e-30 119
MsG0880047049.01.T01 MTR_4g108940 69.231 39 12 0 225 263 247 285 7.00e-12 66.6
MsG0880047049.01.T01 MTR_5g015260 53.933 89 38 1 140 228 90 175 8.28e-25 105
MsG0880047049.01.T01 MTR_5g015260 44.595 74 31 4 262 331 375 442 7.49e-11 63.5
MsG0880047049.01.T01 MTR_4g108950 59.322 59 24 0 251 309 25 83 2.13e-22 90.5
MsG0880047049.01.T01 MTR_4g097240 47.191 89 44 1 140 228 84 169 1.08e-21 95.9
MsG0880047049.01.T01 MTR_4g097230 42.697 89 48 1 140 228 69 154 1.35e-18 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047049.01.T01 AT4G02500 70.659 167 36 3 73 230 41 203 1.91e-73 235
MsG0880047049.01.T01 AT4G02500 77.451 102 23 0 251 352 359 460 2.22e-50 174
MsG0880047049.01.T01 AT4G02500 79.412 34 7 0 231 264 263 296 1.42e-11 65.9
MsG0880047049.01.T01 AT3G62720 69.512 164 42 3 73 230 41 202 1.62e-72 233
MsG0880047049.01.T01 AT3G62720 82.022 89 16 0 264 352 371 459 3.66e-46 163
MsG0880047049.01.T01 AT3G62720 85.294 34 5 0 231 264 262 295 1.02e-12 69.3
MsG0880047049.01.T01 AT3G62720 69.512 164 42 3 73 230 41 202 1.62e-72 233
MsG0880047049.01.T01 AT3G62720 82.022 89 16 0 264 352 371 459 3.66e-46 163
MsG0880047049.01.T01 AT3G62720 85.294 34 5 0 231 264 262 295 1.02e-12 69.3
MsG0880047049.01.T01 AT5G07720 49.677 155 54 4 76 230 72 202 4.98e-40 147
MsG0880047049.01.T01 AT5G07720 51.136 88 43 0 251 338 358 445 1.17e-27 113
MsG0880047049.01.T01 AT5G07720 84.848 33 5 0 231 263 262 294 1.73e-12 68.6
MsG0880047049.01.T01 AT1G74380 54.135 133 44 3 98 230 88 203 3.61e-39 144
MsG0880047049.01.T01 AT1G74380 52.273 88 42 0 251 338 359 446 1.43e-28 115
MsG0880047049.01.T01 AT1G74380 87.879 33 4 0 231 263 263 295 8.43e-13 69.7
MsG0880047049.01.T01 AT1G18690 65.556 90 30 1 141 230 173 261 2.55e-35 135
MsG0880047049.01.T01 AT1G18690 50.000 88 44 0 251 338 417 504 1.62e-27 113
MsG0880047049.01.T01 AT1G18690 79.412 34 7 0 231 264 321 354 3.28e-12 67.8
MsG0880047049.01.T01 AT1G18690 65.556 90 30 1 141 230 173 261 2.55e-35 135
MsG0880047049.01.T01 AT1G18690 50.000 88 44 0 251 338 417 504 1.62e-27 113
MsG0880047049.01.T01 AT1G18690 79.412 34 7 0 231 264 321 354 3.28e-12 67.8
MsG0880047049.01.T01 AT1G18690 65.556 90 30 1 141 230 173 261 2.55e-35 135
MsG0880047049.01.T01 AT1G18690 50.000 88 44 0 251 338 417 504 1.62e-27 113
MsG0880047049.01.T01 AT1G18690 79.412 34 7 0 231 264 321 354 3.28e-12 67.8
MsG0880047049.01.T01 AT1G18690 65.556 90 30 1 141 230 173 261 2.55e-35 135
MsG0880047049.01.T01 AT1G18690 50.000 88 44 0 251 338 417 504 1.62e-27 113
MsG0880047049.01.T01 AT1G18690 79.412 34 7 0 231 264 321 354 3.28e-12 67.8
MsG0880047049.01.T01 AT2G22900 40.833 120 65 2 109 228 61 174 4.45e-21 94.4
MsG0880047049.01.T01 AT4G38310 44.444 90 46 2 140 228 13 99 2.80e-20 85.5
MsG0880047049.01.T01 AT4G37690 43.820 89 47 1 140 228 72 157 2.10e-19 89.4

Find 84 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATCGTGCTTATAATTT+TGG 0.108748 8:+81343715 MsG0880047049.01.T01:CDS
TTCATCCCAATCGGAGATTT+TGG 0.127715 8:-81342884 None:intergenic
AAAAGAACACGCGGCTTATT+AGG 0.228172 8:-81342958 None:intergenic
TGTAAACCTTGTGGGAAATT+TGG 0.244862 8:+81343661 MsG0880047049.01.T01:CDS
AATATTAATGCAATCTTTGC+AGG 0.275772 8:-81341358 None:intergenic
TTAATAAAATTAGGATAATC+AGG 0.279377 8:-81342934 None:intergenic
CACCAGCTTCTTCATCTTTG+AGG 0.290066 8:-81342816 None:intergenic
CACTTGATATTCTTGATGCT+TGG 0.298715 8:+81343361 MsG0880047049.01.T01:CDS
TTATTAGGATTAATAAAATT+AGG 0.299537 8:-81342943 None:intergenic
AGAATAAGATTGATTATTGT+AGG 0.309019 8:+81343049 MsG0880047049.01.T01:CDS
CACCATCGCGCCGGCAAATT+CGG 0.320813 8:+81342646 MsG0880047049.01.T01:CDS
TTCATCTCTAACCTTCCTTT+AGG 0.321220 8:-81343396 None:intergenic
GATTGGATTGGTTTGAATAC+TGG 0.322958 8:+81343309 MsG0880047049.01.T01:CDS
TTCTGGAGTTCCGAATTTGC+CGG 0.333582 8:-81342656 None:intergenic
ACTTGATATTCTTGATGCTT+GGG 0.350845 8:+81343362 MsG0880047049.01.T01:CDS
TGTTTCAAACACCTCTCAAC+AGG 0.353197 8:-81343691 None:intergenic
ATTGATTATTGTAGGCTTCA+TGG 0.357016 8:+81343057 MsG0880047049.01.T01:CDS
TCAGTCCTAATGCGAGTAAT+TGG 0.357483 8:-81341280 None:intergenic
ATCTCGTTGAGGTCTTGTTC+TGG 0.369679 8:-81342673 None:intergenic
CCAAGTGTATAAGGCGTGTT+TGG 0.374134 8:-81342859 None:intergenic
AGAGAAGATGCAGCGTTTGT+AGG 0.374651 8:+81341322 MsG0880047049.01.T01:CDS
GTAGAAGAGTGAAGAGAGTT+AGG 0.375182 8:+81343788 MsG0880047049.01.T01:CDS
ATGATTTATGATGAGAAGAT+TGG 0.381642 8:+81343292 MsG0880047049.01.T01:intron
GGCTTTACTTGATGCTGAAA+TGG 0.404136 8:+81343101 MsG0880047049.01.T01:CDS
CTTGATGTTAAAGATGAACT+TGG 0.406678 8:+81343829 MsG0880047049.01.T01:CDS
AACAAATAATGATCACCAAC+AGG 0.414352 8:-81343015 None:intergenic
AATCAGATATTGCAGATGTA+TGG 0.424424 8:+81343742 MsG0880047049.01.T01:CDS
AACCTTCCTTTAGGTCCCAT+TGG 0.426540 8:-81343387 None:intergenic
TGAGAGGTGTTTGAAACAAA+TGG 0.434956 8:+81343696 MsG0880047049.01.T01:CDS
TCTTGATGCTTGGGCTCCAA+TGG 0.447253 8:+81343371 MsG0880047049.01.T01:CDS
TTGATTATTGTAGGCTTCAT+GGG 0.455567 8:+81343058 MsG0880047049.01.T01:CDS
CTTGATGCTTGGGCTCCAAT+GGG 0.460260 8:+81343372 MsG0880047049.01.T01:CDS
TTCCGAATTTGCCGGCGCGA+TGG 0.466431 8:-81342648 None:intergenic
AACTTGATAGCTTTGAAAGC+AGG 0.479171 8:-81343862 None:intergenic
TTACTTGATGCTGAAATGGC+TGG 0.485492 8:+81343105 MsG0880047049.01.T01:CDS
CCAAACACGCCTTATACACT+TGG 0.489703 8:+81342859 MsG0880047049.01.T01:CDS
TGGTCCCAAAATCTCCGATT+GGG 0.498795 8:+81342879 MsG0880047049.01.T01:CDS
ACCTTTCTTTGCCTTACGCA+TGG 0.500314 8:-81341251 None:intergenic
TTGTGGGGTGTAAACCTTGT+GGG 0.502092 8:+81343653 MsG0880047049.01.T01:CDS
CAAAACTATCAAGAAGAAGT+TGG 0.504847 8:-81343886 None:intergenic
AGTTCATCTTTAACATCAAG+AGG 0.518520 8:-81343826 None:intergenic
GTCCACCAATTACTCGCATT+AGG 0.525154 8:+81341275 MsG0880047049.01.T01:CDS
TTGCCGGCGCGATGGTGCCA+CGG 0.530050 8:-81342640 None:intergenic
TGGTGATCATAGGTGGCCAT+TGG 0.531865 8:+81343621 MsG0880047049.01.T01:intron
TGGGCTCCAATGGGACCTAA+AGG 0.532192 8:+81343381 MsG0880047049.01.T01:CDS
GTCCTAATGCGAGTAATTGG+TGG 0.533049 8:-81341277 None:intergenic
TTGGTCCCAAAATCTCCGAT+TGG 0.536870 8:+81342878 MsG0880047049.01.T01:CDS
TGAACGTTGTTCATCCCAAT+CGG 0.550454 8:-81342893 None:intergenic
TAATAAAATTAGGATAATCA+GGG 0.553025 8:-81342933 None:intergenic
TCCATGCGTAAGGCAAAGAA+AGG 0.563424 8:+81341250 None:intergenic
CTTGATGCTGAAATGGCTGG+TGG 0.566468 8:+81343108 MsG0880047049.01.T01:CDS
TTATGATGAGAAGATTGGAT+TGG 0.567446 8:+81343297 MsG0880047049.01.T01:intron
CCTCCGTGGCACCATCGCGC+CGG 0.570309 8:+81342637 MsG0880047049.01.T01:CDS
ATCCTCAAAGATGAAGAAGC+TGG 0.572972 8:+81342814 MsG0880047049.01.T01:CDS
TTAGGACTGAGATGGACGAT+TGG 0.580063 8:+81341293 MsG0880047049.01.T01:CDS
TCGAGACGAATCCATGCGTA+AGG 0.584613 8:+81341240 None:intergenic
CGCGGTGAGGCTCAACGCGA+CGG 0.585818 8:-81342720 None:intergenic
CAATCTTTGCAGGAATGACA+AGG 0.600645 8:-81341348 None:intergenic
CTCCAATGGGACCTAAAGGA+AGG 0.604098 8:+81343385 MsG0880047049.01.T01:CDS
TACTCGCATTAGGACTGAGA+TGG 0.604185 8:+81341285 MsG0880047049.01.T01:CDS
GATTGTCGAGATATCGAAAG+TGG 0.605889 8:-81342794 None:intergenic
TATAAGGCGTGTTTGGATCG+CGG 0.616876 8:-81342852 None:intergenic
ATGCAGCGCGCATTCCGCCG+CGG 0.620343 8:+81342575 MsG0880047049.01.T01:CDS
GGTGCCACGGAGGACGATGA+CGG 0.623672 8:-81342627 None:intergenic
CACGTGAGTAGAGCTGTTGA+CGG 0.625195 8:-81342696 None:intergenic
GTTTCAAACACCTCTCAACA+GGG 0.632456 8:-81343690 None:intergenic
ACTTCTTCGAGAACGCGGTG+AGG 0.636653 8:-81342733 None:intergenic
CACGGAGGACGATGACGGTG+AGG 0.642282 8:-81342622 None:intergenic
TAATCACCAAATTTCCCACA+AGG 0.642639 8:-81343667 None:intergenic
GGTGAGGAAGAGACAGAGGA+AGG 0.644084 8:-81342606 None:intergenic
AGAGGAAGGTGAGAGTGCCG+CGG 0.644711 8:-81342592 None:intergenic
TGACGGTGAGGAAGAGACAG+AGG 0.652220 8:-81342610 None:intergenic
CCCACAAAATGAGTCACCAA+TGG 0.656603 8:-81343637 None:intergenic
CCAGTAACTAAAAGAACACG+CGG 0.656971 8:-81342967 None:intergenic
TTGGTGATTACCCTGTTGAG+AGG 0.659971 8:+81343680 MsG0880047049.01.T01:CDS
CGTCAACAGCTCTACTCACG+TGG 0.662023 8:+81342697 MsG0880047049.01.T01:CDS
ATTGAACTTCTTCGAGAACG+CGG 0.665143 8:-81342738 None:intergenic
AAACCGTGTGAAAATCCTGT+TGG 0.668048 8:+81343000 MsG0880047049.01.T01:CDS
GGAAGGTGAGAGTGCCGCGG+CGG 0.678059 8:-81342589 None:intergenic
CTCACCGTCATCGTCCTCCG+TGG 0.681669 8:+81342623 MsG0880047049.01.T01:CDS
GCTGTTGACGGATCTCGTTG+AGG 0.685304 8:-81342684 None:intergenic
TTTGTGGGGTGTAAACCTTG+TGG 0.685441 8:+81343652 MsG0880047049.01.T01:CDS
CCGGCGCGATGGTGCCACGG+AGG 0.710066 8:-81342637 None:intergenic
GGGATGAACAACGTTCATCG+TGG 0.722304 8:+81342899 MsG0880047049.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTAAATTTTTTTAAAAA+AGG + Chr8:81341997-81342016 MsG0880047049.01.T01:intron 0.0%
!! AAAAAAATTCGAAAAAAAAT+TGG + Chr8:81342056-81342075 MsG0880047049.01.T01:intron 10.0%
!! TAAATAAAAATGAGATTTAA+GGG - Chr8:81342465-81342484 None:intergenic 10.0%
!! TTATTAGGATTAATAAAATT+AGG - Chr8:81342946-81342965 None:intergenic 10.0%
!!! ATTATTAATATAAGTTGAAA+TGG + Chr8:81341496-81341515 MsG0880047049.01.T01:intron 10.0%
!!! TTATAATATTGTAGATTTTA+TGG + Chr8:81341575-81341594 MsG0880047049.01.T01:intron 10.0%
!! AATATTATGTAAGATATTGA+TGG + Chr8:81341749-81341768 MsG0880047049.01.T01:intron 15.0%
!! GTAAATAAAAATGAGATTTA+AGG - Chr8:81342466-81342485 None:intergenic 15.0%
!! TAATAAAATTAGGATAATCA+GGG - Chr8:81342936-81342955 None:intergenic 15.0%
!! TTAATAAAATTAGGATAATC+AGG - Chr8:81342937-81342956 None:intergenic 15.0%
!!! AATATTGTTTTTTGTCAAAT+AGG - Chr8:81341682-81341701 None:intergenic 15.0%
!! AGAATAAGATTGATTATTGT+AGG + Chr8:81343049-81343068 MsG0880047049.01.T01:CDS 20.0%
!! ATAAAATAAAATAAACGCGT+AGG + Chr8:81342302-81342321 MsG0880047049.01.T01:intron 20.0%
!! ATTATTATTTGCATGGTTAT+TGG + Chr8:81343546-81343565 MsG0880047049.01.T01:intron 20.0%
!! GTGATAAATTGAAAAATGAA+AGG + Chr8:81341520-81341539 MsG0880047049.01.T01:intron 20.0%
!! TCAAAGATAAATATATGCAT+TGG + Chr8:81341840-81341859 MsG0880047049.01.T01:intron 20.0%
!! TTATTATTTGCATGGTTATT+GGG + Chr8:81343547-81343566 MsG0880047049.01.T01:intron 20.0%
!! TTGTGAATTAGTAGAAAATA+TGG - Chr8:81341721-81341740 None:intergenic 20.0%
!!! ACAAAAATTTGTCACTTTTA+GGG + Chr8:81342117-81342136 MsG0880047049.01.T01:intron 20.0%
! AATATTAATGCAATCTTTGC+AGG - Chr8:81341361-81341380 None:intergenic 25.0%
! ATGATTTATGATGAGAAGAT+TGG + Chr8:81343292-81343311 MsG0880047049.01.T01:intron 25.0%
! GAGAATCATTATTATTTGCA+TGG + Chr8:81343539-81343558 MsG0880047049.01.T01:intron 25.0%
! TATTATTTGCATGGTTATTG+GGG + Chr8:81343548-81343567 MsG0880047049.01.T01:intron 25.0%
!! GATTGAGATTTTCTATAACA+TGG + Chr8:81343080-81343099 MsG0880047049.01.T01:CDS 25.0%
!!! GACAAAAATTTGTCACTTTT+AGG + Chr8:81342116-81342135 MsG0880047049.01.T01:intron 25.0%
!!! GCAATTTTCTGAAAAAAGTA+GGG + Chr8:81342196-81342215 MsG0880047049.01.T01:intron 25.0%
!!! TGCAATTTTCTGAAAAAAGT+AGG + Chr8:81342195-81342214 MsG0880047049.01.T01:intron 25.0%
!!! TTAAGGGTGTTTTGTTTTTT+CGG - Chr8:81342449-81342468 None:intergenic 25.0%
!!! TTGGATTTTTTTGGCAAAAA+GGG + Chr8:81342224-81342243 MsG0880047049.01.T01:intron 25.0%
!!! TTTGGATTTTTTTGGCAAAA+AGG + Chr8:81342223-81342242 MsG0880047049.01.T01:intron 25.0%
AACAAATAATGATCACCAAC+AGG - Chr8:81343018-81343037 None:intergenic 30.0%
AACAATAGCTTCCTAATCAA+AGG - Chr8:81343149-81343168 None:intergenic 30.0%
AATCAGATATTGCAGATGTA+TGG + Chr8:81343742-81343761 MsG0880047049.01.T01:CDS 30.0%
AATTTAAATACCCCTCTACT+TGG - Chr8:81341987-81342006 None:intergenic 30.0%
ACCATAAAGTCAGAAAAGTA+GGG + Chr8:81342166-81342185 MsG0880047049.01.T01:intron 30.0%
ACTTGATATTCTTGATGCTT+GGG + Chr8:81343362-81343381 MsG0880047049.01.T01:CDS 30.0%
AGAAGTGATGAAGTGAATTA+TGG - Chr8:81342376-81342395 None:intergenic 30.0%
AGTTCATCTTTAACATCAAG+AGG - Chr8:81343829-81343848 None:intergenic 30.0%
ATCTGCTATGGTTTATTTGT+TGG + Chr8:81343478-81343497 MsG0880047049.01.T01:intron 30.0%
ATCTTATCTTACGTATACGT+AGG - Chr8:81342269-81342288 None:intergenic 30.0%
ATTATTTGCATGGTTATTGG+GGG + Chr8:81343549-81343568 MsG0880047049.01.T01:intron 30.0%
ATTGATTATTGTAGGCTTCA+TGG + Chr8:81343057-81343076 MsG0880047049.01.T01:CDS 30.0%
CTTGATGTTAAAGATGAACT+TGG + Chr8:81343829-81343848 MsG0880047049.01.T01:CDS 30.0%
GATGATTGAGAATTATCATC+TGG + Chr8:81343595-81343614 MsG0880047049.01.T01:intron 30.0%
TACCATAAAGTCAGAAAAGT+AGG + Chr8:81342165-81342184 MsG0880047049.01.T01:intron 30.0%
TCAACTAGGTCATCTAATAT+GGG + Chr8:81341877-81341896 MsG0880047049.01.T01:intron 30.0%
TCTTATCTTACGTATACGTA+GGG - Chr8:81342268-81342287 None:intergenic 30.0%
TGATGCTATGTTTACTGATA+TGG + Chr8:81343208-81343227 MsG0880047049.01.T01:intron 30.0%
TTATGATGAGAAGATTGGAT+TGG + Chr8:81343297-81343316 MsG0880047049.01.T01:intron 30.0%
TTGATTATTGTAGGCTTCAT+GGG + Chr8:81343058-81343077 MsG0880047049.01.T01:CDS 30.0%
! ATGGATCGTGCTTATAATTT+TGG + Chr8:81343715-81343734 MsG0880047049.01.T01:CDS 30.0%
! CTATCTCTGATCTTAATTTG+TGG + Chr8:81342405-81342424 MsG0880047049.01.T01:intron 30.0%
! TGAAAAAAGTAGGGTAGTTT+TGG + Chr8:81342205-81342224 MsG0880047049.01.T01:intron 30.0%
!! TGAGAATTATCATCTGGTTT+TGG + Chr8:81343601-81343620 MsG0880047049.01.T01:intron 30.0%
!!! AGGGTAGTTTTGGATTTTTT+TGG + Chr8:81342215-81342234 MsG0880047049.01.T01:intron 30.0%
!!! ATACTGGAAGTTTTTTGTTG+AGG + Chr8:81343325-81343344 MsG0880047049.01.T01:CDS 30.0%
!!! TGGATTTTTTTGGCAAAAAG+GGG + Chr8:81342225-81342244 MsG0880047049.01.T01:intron 30.0%
ATTAGAGAGAGAGAAAAGAG+TGG + Chr8:81342331-81342350 MsG0880047049.01.T01:intron 35.0%
ATTGGAAAATACCCTCAACT+TGG + Chr8:81342074-81342093 MsG0880047049.01.T01:intron 35.0%
CACTTGATATTCTTGATGCT+TGG + Chr8:81343361-81343380 MsG0880047049.01.T01:CDS 35.0%
GTCAAATAGGTCATCTATTG+AGG - Chr8:81341669-81341688 None:intergenic 35.0%
GTCAACTAGGTCATCTAATA+TGG + Chr8:81341876-81341895 MsG0880047049.01.T01:intron 35.0%
TAATCACCAAATTTCCCACA+AGG - Chr8:81343670-81343689 None:intergenic 35.0%
TGGAGTAGAGTGGTATAATA+AGG - Chr8:81341967-81341986 None:intergenic 35.0%
TGTAAACCTTGTGGGAAATT+TGG + Chr8:81343661-81343680 MsG0880047049.01.T01:CDS 35.0%
TTCATCTCTAACCTTCCTTT+AGG - Chr8:81343399-81343418 None:intergenic 35.0%
! ATCCTGAAGTTGAGTTTTTG+TGG + Chr8:81343174-81343193 MsG0880047049.01.T01:intron 35.0%
! ATGGTTTATTTGTTGGCAAC+TGG + Chr8:81343485-81343504 MsG0880047049.01.T01:intron 35.0%
! CATAATTTGTGATGCATGGT+TGG + Chr8:81343263-81343282 MsG0880047049.01.T01:intron 35.0%
! GGATCATAATTTGTGATGCA+TGG + Chr8:81343259-81343278 MsG0880047049.01.T01:intron 35.0%
! GTTTACTGATATGGCTTTTG+AGG + Chr8:81343217-81343236 MsG0880047049.01.T01:intron 35.0%
! TGGTTTATTTGTTGGCAACT+GGG + Chr8:81343486-81343505 MsG0880047049.01.T01:intron 35.0%
! TTTTGTTGAGGAATTGTCAG+TGG + Chr8:81343337-81343356 MsG0880047049.01.T01:CDS 35.0%
!! AACTTGATAGCTTTGAAAGC+AGG - Chr8:81343865-81343884 None:intergenic 35.0%
!! ACCCTACTTTTCTGACTTTA+TGG - Chr8:81342170-81342189 None:intergenic 35.0%
!! GATTGGATTGGTTTGAATAC+TGG + Chr8:81343309-81343328 MsG0880047049.01.T01:CDS 35.0%
!! TGAGAGGTGTTTGAAACAAA+TGG + Chr8:81343696-81343715 MsG0880047049.01.T01:CDS 35.0%
!!! ATTTTGGGACCAAGTGTATA+AGG - Chr8:81342871-81342890 None:intergenic 35.0%
!!! GTTATCGTTGTTGTTTTCTG+TGG - Chr8:81342764-81342783 None:intergenic 35.0%
!!! TTTTTTACTACCACTCAACC+TGG - Chr8:81342042-81342061 None:intergenic 35.0%
AAAAGAACACGCGGCTTATT+AGG - Chr8:81342961-81342980 None:intergenic 40.0%
AAACCGTGTGAAAATCCTGT+TGG + Chr8:81343000-81343019 MsG0880047049.01.T01:CDS 40.0%
AAGCTGGGAAGATTCTTACT+AGG + Chr8:81343417-81343436 MsG0880047049.01.T01:intron 40.0%
AGCAGATGATCAATCTGCTA+TGG + Chr8:81343466-81343485 MsG0880047049.01.T01:intron 40.0%
AGCTGGGAAGATTCTTACTA+GGG + Chr8:81343418-81343437 MsG0880047049.01.T01:intron 40.0%
ATCCTCAAAGATGAAGAAGC+TGG + Chr8:81342814-81342833 MsG0880047049.01.T01:CDS 40.0%
ATTGAACTTCTTCGAGAACG+CGG - Chr8:81342741-81342760 None:intergenic 40.0%
CAATCTTTGCAGGAATGACA+AGG - Chr8:81341351-81341370 None:intergenic 40.0%
CACCACAAAAACTCAACTTC+AGG - Chr8:81343179-81343198 None:intergenic 40.0%
CATATATACAACGCAAGTCC+AGG + Chr8:81342021-81342040 MsG0880047049.01.T01:intron 40.0%
CCAGTAACTAAAAGAACACG+CGG - Chr8:81342970-81342989 None:intergenic 40.0%
CTAGGTCATCTAATATGGGA+CGG + Chr8:81341881-81341900 MsG0880047049.01.T01:intron 40.0%
GATTGTCGAGATATCGAAAG+TGG - Chr8:81342797-81342816 None:intergenic 40.0%
GGCTTTACTTGATGCTGAAA+TGG + Chr8:81343101-81343120 MsG0880047049.01.T01:CDS 40.0%
GTAGAAGAGTGAAGAGAGTT+AGG + Chr8:81343788-81343807 MsG0880047049.01.T01:CDS 40.0%
GTTTCAAACACCTCTCAACA+GGG - Chr8:81343693-81343712 None:intergenic 40.0%
TAGGGAGTTAAAGAATCGAC+CGG + Chr8:81343436-81343455 MsG0880047049.01.T01:intron 40.0%
TAGGTCATCTATTGAGGAGA+CGG - Chr8:81341663-81341682 None:intergenic 40.0%
TCAGTCCTAATGCGAGTAAT+TGG - Chr8:81341283-81341302 None:intergenic 40.0%
TGAACGTTGTTCATCCCAAT+CGG - Chr8:81342896-81342915 None:intergenic 40.0%
TGATCATCTGCTTCGAAAAC+CGG - Chr8:81343458-81343477 None:intergenic 40.0%
TGTTTCAAACACCTCTCAAC+AGG - Chr8:81343694-81343713 None:intergenic 40.0%
TTACTTGATGCTGAAATGGC+TGG + Chr8:81343105-81343124 MsG0880047049.01.T01:CDS 40.0%
! ACAGGATTTTCACACGGTTT+AGG - Chr8:81343000-81343019 None:intergenic 40.0%
! CTGAAGTTGAGTTTTTGTGG+TGG + Chr8:81343177-81343196 MsG0880047049.01.T01:intron 40.0%
! TCACCAACAGGATTTTCACA+CGG - Chr8:81343006-81343025 None:intergenic 40.0%
! TCATCCCAATCGGAGATTTT+GGG - Chr8:81342886-81342905 None:intergenic 40.0%
! TTATTTGTTGGCAACTGGGA+GGG + Chr8:81343490-81343509 MsG0880047049.01.T01:intron 40.0%
! TTCATCCCAATCGGAGATTT+TGG - Chr8:81342887-81342906 None:intergenic 40.0%
! TTTATTTGTTGGCAACTGGG+AGG + Chr8:81343489-81343508 MsG0880047049.01.T01:intron 40.0%
!! AGTTGAGTTTTTGTGGTGGA+TGG + Chr8:81343181-81343200 MsG0880047049.01.T01:intron 40.0%
!! CATTGGTGACTCATTTTGTG+GGG + Chr8:81343638-81343657 MsG0880047049.01.T01:CDS 40.0%
!! CCATTGGTGACTCATTTTGT+GGG + Chr8:81343637-81343656 MsG0880047049.01.T01:CDS 40.0%
!!! AGGGTGTTTTGTTTTTTCGG+TGG - Chr8:81342446-81342465 None:intergenic 40.0%
!!! CATCTGGTTTTGGTGATCAT+AGG + Chr8:81343611-81343630 MsG0880047049.01.T01:intron 40.0%
AACCTTCCTTTAGGTCCCAT+TGG - Chr8:81343390-81343409 None:intergenic 45.0%
ACCACTCTACTCCAAGTAGA+GGG + Chr8:81341973-81341992 MsG0880047049.01.T01:intron 45.0%
AGAGAAGATGCAGCGTTTGT+AGG + Chr8:81341322-81341341 MsG0880047049.01.T01:CDS 45.0%
AGGAAGGTTAGAGATGAAGC+TGG + Chr8:81343401-81343420 MsG0880047049.01.T01:intron 45.0%
ATACCCTCAACTTGGAGTAG+AGG + Chr8:81342082-81342101 MsG0880047049.01.T01:intron 45.0%
CACCAGCTTCTTCATCTTTG+AGG - Chr8:81342819-81342838 None:intergenic 45.0%
CCAAACACGCCTTATACACT+TGG + Chr8:81342859-81342878 MsG0880047049.01.T01:CDS 45.0%
CCAAGTGTATAAGGCGTGTT+TGG - Chr8:81342862-81342881 None:intergenic 45.0%
CCCACAAAATGAGTCACCAA+TGG - Chr8:81343640-81343659 None:intergenic 45.0%
CTTTATGGTACCACTCCACT+TGG - Chr8:81342155-81342174 None:intergenic 45.0%
GGAAGGTTAGAGATGAAGCT+GGG + Chr8:81343402-81343421 MsG0880047049.01.T01:intron 45.0%
GGGCTAAGCTTCCTTTGATT+AGG + Chr8:81343135-81343154 MsG0880047049.01.T01:intron 45.0%
GTCCACCAATTACTCGCATT+AGG + Chr8:81341275-81341294 MsG0880047049.01.T01:CDS 45.0%
GTCCTAATGCGAGTAATTGG+TGG - Chr8:81341280-81341299 None:intergenic 45.0%
TACCACTCTACTCCAAGTAG+AGG + Chr8:81341972-81341991 MsG0880047049.01.T01:intron 45.0%
TACCCTCAACTTGGAGTAGA+GGG + Chr8:81342083-81342102 MsG0880047049.01.T01:intron 45.0%
TACGTGTGTCAGAGTCAACT+AGG + Chr8:81341863-81341882 MsG0880047049.01.T01:intron 45.0%
TACTCGCATTAGGACTGAGA+TGG + Chr8:81341285-81341304 MsG0880047049.01.T01:CDS 45.0%
TATAAGGCGTGTTTGGATCG+CGG - Chr8:81342855-81342874 None:intergenic 45.0%
TGATCCTTTAAACGCTCCCA+CGG - Chr8:81343245-81343264 None:intergenic 45.0%
TGGTCCCAAAATCTCCGATT+GGG + Chr8:81342879-81342898 MsG0880047049.01.T01:CDS 45.0%
TTAGGACTGAGATGGACGAT+TGG + Chr8:81341293-81341312 MsG0880047049.01.T01:CDS 45.0%
TTGGTCCCAAAATCTCCGAT+TGG + Chr8:81342878-81342897 MsG0880047049.01.T01:CDS 45.0%
TTGTGGGGTGTAAACCTTGT+GGG + Chr8:81343653-81343672 MsG0880047049.01.T01:CDS 45.0%
TTTGTGGGGTGTAAACCTTG+TGG + Chr8:81343652-81343671 MsG0880047049.01.T01:CDS 45.0%
! CTGAAATGGCTGGTGGTTTT+GGG + Chr8:81343115-81343134 MsG0880047049.01.T01:intron 45.0%
! GCATGGTTATTGGGGGATTT+TGG + Chr8:81343556-81343575 MsG0880047049.01.T01:intron 45.0%
! TGGAGTAGAGGGGTACAATA+AGG + Chr8:81342094-81342113 MsG0880047049.01.T01:intron 45.0%
! TTGGTGATTACCCTGTTGAG+AGG + Chr8:81343680-81343699 MsG0880047049.01.T01:CDS 45.0%
!! ATCTCGTTGAGGTCTTGTTC+TGG - Chr8:81342676-81342695 None:intergenic 45.0%
!! CCGCGTGTTCTTTTAGTTAC+TGG + Chr8:81342967-81342986 MsG0880047049.01.T01:CDS 45.0%
!! GCCATTGGTGACTCATTTTG+TGG + Chr8:81343636-81343655 MsG0880047049.01.T01:CDS 45.0%
!! TTCTGGAGTTCCGAATTTGC+CGG - Chr8:81342659-81342678 None:intergenic 45.0%
!!! ATATGGCTTTTGAGGTTCCG+TGG + Chr8:81343225-81343244 MsG0880047049.01.T01:intron 45.0%
!!! CTGGTTTTGGTGATCATAGG+TGG + Chr8:81343614-81343633 MsG0880047049.01.T01:intron 45.0%
!!! TATGGCTTTTGAGGTTCCGT+GGG + Chr8:81343226-81343245 MsG0880047049.01.T01:intron 45.0%
!! TATATTAATAATGTTAATTT+AGG - Chr8:81341488-81341507 None:intergenic 5.0%
ACCCTCAACTTGGAGTAGAG+GGG + Chr8:81342084-81342103 MsG0880047049.01.T01:intron 50.0%
AGAGAGAAGGAGAGGGTGAT+GGG - Chr8:81342496-81342515 None:intergenic 50.0%
CACGTGAGTAGAGCTGTTGA+CGG - Chr8:81342699-81342718 None:intergenic 50.0%
CCACTCTACTCCAAGTAGAG+GGG + Chr8:81341974-81341993 MsG0880047049.01.T01:intron 50.0%
CTCACACAACAATGCTGGAG+CGG + Chr8:81342525-81342544 MsG0880047049.01.T01:intron 50.0%
CTCCAATGGGACCTAAAGGA+AGG + Chr8:81343385-81343404 MsG0880047049.01.T01:CDS 50.0%
CTTGATGCTGAAATGGCTGG+TGG + Chr8:81343108-81343127 MsG0880047049.01.T01:CDS 50.0%
CTTGATGCTTGGGCTCCAAT+GGG + Chr8:81343372-81343391 MsG0880047049.01.T01:CDS 50.0%
GGGATGAACAACGTTCATCG+TGG + Chr8:81342899-81342918 MsG0880047049.01.T01:CDS 50.0%
GTCATCTATTGAGGAGACGG+AGG - Chr8:81341660-81341679 None:intergenic 50.0%
TACCACTCCACTTGGAGTTG+CGG - Chr8:81342147-81342166 None:intergenic 50.0%
TACCCCTCTACTCCAAGTTG+AGG - Chr8:81342089-81342108 None:intergenic 50.0%
TCTTGATGCTTGGGCTCCAA+TGG + Chr8:81343371-81343390 MsG0880047049.01.T01:CDS 50.0%
TGGTGATCATAGGTGGCCAT+TGG + Chr8:81343621-81343640 MsG0880047049.01.T01:intron 50.0%
! ACCCCTCTACTCCAAGTTGA+GGG - Chr8:81342088-81342107 None:intergenic 50.0%
! GCTGAAATGGCTGGTGGTTT+TGG + Chr8:81343114-81343133 MsG0880047049.01.T01:intron 50.0%
ACAATGCTGGAGCGGTGTCT+CGG + Chr8:81342533-81342552 MsG0880047049.01.T01:intron 55.0%
ACCACTCCACTTGGAGTTGC+GGG - Chr8:81342146-81342165 None:intergenic 55.0%
ACTTCTTCGAGAACGCGGTG+AGG - Chr8:81342736-81342755 None:intergenic 55.0%
CAGAGAGAAGGAGAGGGTGA+TGG - Chr8:81342497-81342516 None:intergenic 55.0%
CCCCTCTACTTGGAGTAGAG+TGG - Chr8:81341977-81341996 None:intergenic 55.0%
CGCACCTCACACAACAATGC+TGG + Chr8:81342520-81342539 MsG0880047049.01.T01:intron 55.0%
CGCTCCAGCATTGTTGTGTG+AGG - Chr8:81342527-81342546 None:intergenic 55.0%
CGTCAACAGCTCTACTCACG+TGG + Chr8:81342697-81342716 MsG0880047049.01.T01:CDS 55.0%
GCAACTGGGAGGGAACAATG+GGG + Chr8:81343500-81343519 MsG0880047049.01.T01:intron 55.0%
GGCAACTGGGAGGGAACAAT+GGG + Chr8:81343499-81343518 MsG0880047049.01.T01:intron 55.0%
GGTGAGGAAGAGACAGAGGA+AGG - Chr8:81342609-81342628 None:intergenic 55.0%
TGACGGTGAGGAAGAGACAG+AGG - Chr8:81342613-81342632 None:intergenic 55.0%
TGGCAACTGGGAGGGAACAA+TGG + Chr8:81343498-81343517 MsG0880047049.01.T01:intron 55.0%
TGGGCTCCAATGGGACCTAA+AGG + Chr8:81343381-81343400 MsG0880047049.01.T01:CDS 55.0%
! CAACGCAAGTCCAGGTTGAG+TGG + Chr8:81342029-81342048 MsG0880047049.01.T01:intron 55.0%
! GCTGTTGACGGATCTCGTTG+AGG - Chr8:81342687-81342706 None:intergenic 55.0%
!! GGTTCCGTGGGAGCGTTTAA+AGG + Chr8:81343238-81343257 MsG0880047049.01.T01:intron 55.0%
ACTGGGAGGGAACAATGGGG+TGG + Chr8:81343503-81343522 MsG0880047049.01.T01:intron 60.0%
CCACTCCACTTGGAGTTGCG+GGG - Chr8:81342145-81342164 None:intergenic 60.0%
CTGGGAGGGAACAATGGGGT+GGG + Chr8:81343504-81343523 MsG0880047049.01.T01:intron 60.0%
GCGACGCAGAGAGAAGGAGA+GGG - Chr8:81342503-81342522 None:intergenic 60.0%
GGAATGCGCGCTGCATCTGA+CGG - Chr8:81342571-81342590 None:intergenic 60.0%
TGAGGTGCGACGCAGAGAGA+AGG - Chr8:81342509-81342528 None:intergenic 60.0%
TGCGACGCAGAGAGAAGGAG+AGG - Chr8:81342504-81342523 None:intergenic 60.0%
TTCCGAATTTGCCGGCGCGA+TGG - Chr8:81342651-81342670 None:intergenic 60.0%
! CACCATCGCGCCGGCAAATT+CGG + Chr8:81342646-81342665 MsG0880047049.01.T01:CDS 60.0%
!! AGAGGAAGGTGAGAGTGCCG+CGG - Chr8:81342595-81342614 None:intergenic 60.0%
CCCCGCAACTCCAAGTGGAG+TGG + Chr8:81342142-81342161 MsG0880047049.01.T01:intron 65.0%
CGCGCTGCATCTGACGGAGA+CGG - Chr8:81342565-81342584 None:intergenic 65.0%
CTCACCGTCATCGTCCTCCG+TGG + Chr8:81342623-81342642 MsG0880047049.01.T01:CDS 65.0%
GGGTACCCCGCAACTCCAAG+TGG + Chr8:81342137-81342156 MsG0880047049.01.T01:intron 65.0%
! CACGGAGGACGATGACGGTG+AGG - Chr8:81342625-81342644 None:intergenic 65.0%
! GGTGCCACGGAGGACGATGA+CGG - Chr8:81342630-81342649 None:intergenic 65.0%
ATGCAGCGCGCATTCCGCCG+CGG + Chr8:81342575-81342594 MsG0880047049.01.T01:CDS 70.0%
CGCGGTGAGGCTCAACGCGA+CGG - Chr8:81342723-81342742 None:intergenic 70.0%
! TTGCCGGCGCGATGGTGCCA+CGG - Chr8:81342643-81342662 None:intergenic 70.0%
!! GGAAGGTGAGAGTGCCGCGG+CGG - Chr8:81342592-81342611 None:intergenic 70.0%
CCTCCGTGGCACCATCGCGC+CGG + Chr8:81342637-81342656 MsG0880047049.01.T01:CDS 75.0%
!!! CCGGCGCGATGGTGCCACGG+AGG - Chr8:81342640-81342659 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr8 gene 81341253 81343900 81341253 ID=MsG0880047049.01;Name=MsG0880047049.01
Chr8 mRNA 81341253 81343900 81341253 ID=MsG0880047049.01.T01;Parent=MsG0880047049.01;Name=MsG0880047049.01.T01;_AED=0.48;_eAED=0.53;_QI=0|0|0|0.75|1|1|4|0|352
Chr8 exon 81341253 81341388 81341253 ID=MsG0880047049.01.T01:exon:10619;Parent=MsG0880047049.01.T01
Chr8 exon 81342575 81343129 81342575 ID=MsG0880047049.01.T01:exon:10620;Parent=MsG0880047049.01.T01
Chr8 exon 81343307 81343406 81343307 ID=MsG0880047049.01.T01:exon:10621;Parent=MsG0880047049.01.T01
Chr8 exon 81343633 81343900 81343633 ID=MsG0880047049.01.T01:exon:10622;Parent=MsG0880047049.01.T01
Chr8 CDS 81341253 81341388 81341253 ID=MsG0880047049.01.T01:cds;Parent=MsG0880047049.01.T01
Chr8 CDS 81342575 81343129 81342575 ID=MsG0880047049.01.T01:cds;Parent=MsG0880047049.01.T01
Chr8 CDS 81343307 81343406 81343307 ID=MsG0880047049.01.T01:cds;Parent=MsG0880047049.01.T01
Chr8 CDS 81343633 81343900 81343633 ID=MsG0880047049.01.T01:cds;Parent=MsG0880047049.01.T01
Gene Sequence

>MsG0880047049.01.T01

ATGCGTAAGGCAAAGAAAGGTCGTCCACCAATTACTCGCATTAGGACTGAGATGGACGATTGGAAACTGAGAGAAGATGCAGCGTTTGTAGGATGCCTTGTCATTCCTGCAAAGATTGCATTAATATTAGACATCAATGCAGCGCGCATTCCGCCGCGGCACTCTCACCTTCCTCTGTCTCTTCCTCACCGTCATCGTCCTCCGTGGCACCATCGCGCCGGCAAATTCGGAACTCCAGAACAAGACCTCAACGAGATCCGTCAACAGCTCTACTCACGTGGCCGTCGCGTTGAGCCTCACCGCGTTCTCGAAGAAGTTCAATCCACAGAAAACAACAACGATAACAACAACTACGCCACTTTCGATATCTCGACAATCCTCAAAGATGAAGAAGCTGGTGATGATGAAAAACGCCGCGATCCAAACACGCCTTATACACTTGGTCCCAAAATCTCCGATTGGGATGAACAACGTTCATCGTGGCTGAGTAACAACCCTGATTATCCTAATTTTATTAATCCTAATAAGCCGCGTGTTCTTTTAGTTACTGGTTCTTCACCTAAACCGTGTGAAAATCCTGTTGGTGATCATTATTTGTTGAAATCAATTAAGAATAAGATTGATTATTGTAGGCTTCATGGGATTGAGATTTTCTATAACATGGCTTTACTTGATGCTGAAATGGCTGGTGAAGATTGGATTGGTTTGAATACTGGAAGTTTTTTGTTGAGGAATTGTCAGTGGTCACTTGATATTCTTGATGCTTGGGCTCCAATGGGACCTAAAGGAAGGTGGCCATTGGTGACTCATTTTGTGGGGTGTAAACCTTGTGGGAAATTTGGTGATTACCCTGTTGAGAGGTGTTTGAAACAAATGGATCGTGCTTATAATTTTGGTGATAATCAGATATTGCAGATGTATGGTTTTACTCATAAGTCACTTGCTAGTAGAAGAGTGAAGAGAGTTAGGAATGAGAGTAGTAATCCTCTTGATGTTAAAGATGAACTTGGATTGCTTCATCCTGCTTTCAAAGCTATCAAGTTGCCAACTTCTTCTTGA
Protein sequence

>MsG0880047049.01.T01

MRKAKKGRPPITRIRTEMDDWKLREDAAFVGCLVIPAKIALILDINAARIPPRHSHLPLSLPHRHRPPWHHRAGKFGTPEQDLNEIRQQLYSRGRRVEPHRVLEEVQSTENNNDNNNYATFDISTILKDEEAGDDEKRRDPNTPYTLGPKISDWDEQRSSWLSNNPDYPNFINPNKPRVLLVTGSSPKPCENPVGDHYLLKSIKNKIDYCRLHGIEIFYNMALLDAEMAGEDWIGLNTGSFLLRNCQWSLDILDAWAPMGPKGRWPLVTHFVGCKPCGKFGDYPVERCLKQMDRAYNFGDNQILQMYGFTHKSLASRRVKRVRNESSNPLDVKDELGLLHPAFKAIKLPTSS*