AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040703.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040703.01.T01 MTR_7g096220 95.109 184 9 0 18 201 18 201 4.13e-125 351
MsG0780040703.01.T01 MTR_1g070480 56.784 199 79 3 3 201 2 193 1.80e-62 192
MsG0780040703.01.T01 MTR_5g083490 40.513 195 105 4 5 189 10 203 5.22e-39 133
MsG0780040703.01.T01 MTR_2g100260 45.333 150 76 2 32 176 30 178 5.28e-38 130
MsG0780040703.01.T01 MTR_1g080760 54.430 158 64 2 39 193 35 187 2.00e-36 126
MsG0780040703.01.T01 MTR_2g100270 49.133 173 84 2 10 178 3 175 1.28e-34 121
MsG0780040703.01.T01 MTR_4g014060 39.264 163 97 1 36 196 57 219 1.46e-34 122
MsG0780040703.01.T01 MTR_0562s0010 39.264 163 97 1 36 196 57 219 1.46e-34 122
MsG0780040703.01.T01 MTR_8g016150 48.765 162 81 2 11 170 5 166 4.25e-33 117
MsG0780040703.01.T01 MTR_6g092690 39.752 161 93 3 39 197 37 195 9.15e-33 117
MsG0780040703.01.T01 MTR_1g093090 38.854 157 93 2 36 189 20 176 1.00e-19 82.8
MsG0780040703.01.T01 MTR_3g012070 56.842 95 38 3 101 194 31 123 1.55e-19 82.4
MsG0780040703.01.T01 MTR_3g031070 36.879 141 87 1 38 176 28 168 1.92e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040703.01.T01 AT1G55190 42.775 173 94 2 10 177 3 175 2.36e-42 141
MsG0780040703.01.T01 AT1G04260 40.361 166 99 0 30 195 12 177 2.97e-42 140
MsG0780040703.01.T01 AT1G08770 41.566 166 95 1 28 191 37 202 1.18e-39 135
MsG0780040703.01.T01 AT3G13720 41.040 173 97 2 10 177 3 175 2.89e-37 128
MsG0780040703.01.T01 AT5G07110 42.667 150 84 1 35 182 41 190 1.09e-36 127
MsG0780040703.01.T01 AT2G38360 38.095 189 105 5 12 189 19 206 1.25e-36 127
MsG0780040703.01.T01 AT3G13710 42.254 142 82 0 36 177 34 175 6.85e-36 124
MsG0780040703.01.T01 AT2G40380 44.805 154 82 2 38 189 46 198 8.75e-36 125
MsG0780040703.01.T01 AT5G01640 38.816 152 90 2 39 188 55 205 2.31e-35 124
MsG0780040703.01.T01 AT3G56110 41.060 151 87 1 36 184 42 192 3.26e-34 121
MsG0780040703.01.T01 AT3G56110 41.060 151 87 1 36 184 42 192 3.26e-34 121
MsG0780040703.01.T01 AT3G56110 41.060 151 87 1 36 184 42 192 3.26e-34 121
MsG0780040703.01.T01 AT3G56110 41.060 151 87 1 36 184 42 192 3.26e-34 121
MsG0780040703.01.T01 AT5G05380 43.709 151 83 1 36 184 45 195 5.41e-32 115
MsG0780040703.01.T01 AT1G17700 39.053 169 99 2 12 176 6 174 1.34e-31 113
MsG0780040703.01.T01 AT5G05380 43.709 151 83 1 36 184 88 238 1.42e-31 115
MsG0780040703.01.T01 AT5G05380 43.709 151 83 1 36 184 96 246 1.50e-31 115
MsG0780040703.01.T01 AT5G05380 43.709 151 83 1 36 184 112 262 3.15e-31 115
MsG0780040703.01.T01 AT4G29658 38.028 142 87 1 36 176 32 173 2.81e-24 94.7
MsG0780040703.01.T01 AT5G56230 32.450 151 101 1 36 185 33 183 1.12e-23 93.2
MsG0780040703.01.T01 AT1G55640 35.211 142 92 0 35 176 35 176 6.16e-23 91.3
MsG0780040703.01.T01 AT4G29658 37.963 108 66 1 70 176 3 110 2.84e-13 64.3
MsG0780040703.01.T01 AT4G00005 32.000 100 66 1 61 158 2 101 2.62e-11 58.9

Find 62 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGGGCGTGAGATGAAATT+TGG 0.290458 7:+81397603 None:intergenic
GATTGCTTAATTCGTTTGTT+GGG 0.318088 7:-81397125 MsG0780040703.01.T01:CDS
AAAATAAAGAACCTATATTA+AGG 0.327117 7:+81397071 None:intergenic
CGCGTGGCGGAGAGGGGTTT+TGG 0.349042 7:+81397576 None:intergenic
GGATTGCTTAATTCGTTTGT+TGG 0.355051 7:-81397126 MsG0780040703.01.T01:CDS
GTGGCTTCGGTGAGAGAGTA+TGG 0.355861 7:+81397510 None:intergenic
TCTCCGCCACGCGCCGTCCA+TGG 0.370614 7:-81397566 MsG0780040703.01.T01:CDS
TTGGGTGAATGTGTTGGTTT+CGG 0.377231 7:-81397238 MsG0780040703.01.T01:CDS
TGGACGGCGCGTGGCGGAGA+GGG 0.393049 7:+81397569 None:intergenic
GAATGTGTTGGTTTCGGTTG+TGG 0.404968 7:-81397232 MsG0780040703.01.T01:CDS
TTGTGTTGCATGGTGCGTTT+AGG 0.406968 7:-81397194 MsG0780040703.01.T01:CDS
GACGAACCGATTGTTCTGTT+TGG 0.413004 7:-81397336 MsG0780040703.01.T01:CDS
CTTCACGCGCGAGGTGGCTT+CGG 0.424427 7:+81397497 None:intergenic
TCTTGATCACACTGATAGCT+TGG 0.436154 7:-81397380 MsG0780040703.01.T01:CDS
CTCCGCCACGCGCCGTCCAT+GGG 0.449853 7:-81397565 MsG0780040703.01.T01:CDS
GGAGGAATGGAATTTGATGA+TGG 0.455858 7:+81397624 None:intergenic
TTCGGTTGTGGTTGGTGTTG+TGG 0.456074 7:-81397220 MsG0780040703.01.T01:CDS
GTTGTGGTTGTTGTGTTGCA+TGG 0.460537 7:-81397204 MsG0780040703.01.T01:CDS
TTCTGCGGCGGTGCTTGCGG+CGG 0.469986 7:-81397292 MsG0780040703.01.T01:CDS
AAGAAGACGATGATTGAGAT+AGG 0.475530 7:+81397399 None:intergenic
ACCCCTACAAAACGTGGTGC+AGG 0.490203 7:-81397099 MsG0780040703.01.T01:CDS
AGCTTCTGCGGCGGTGCTTG+CGG 0.491539 7:-81397295 MsG0780040703.01.T01:CDS
TGGTGTTTGGGTGAATGTGT+TGG 0.494101 7:-81397244 MsG0780040703.01.T01:CDS
TGAGATGAAATTTGGAGGAA+TGG 0.499497 7:+81397611 None:intergenic
GGAATAAGACGAAGAGGATG+AGG 0.506318 7:+81397439 None:intergenic
ACACGGCCAAACAGAACAAT+CGG 0.514431 7:+81397330 None:intergenic
ATTGCTTAATTCGTTTGTTG+GGG 0.514787 7:-81397124 MsG0780040703.01.T01:CDS
TCTTATTCCTCAGTCTCCTA+TGG 0.515363 7:-81397425 MsG0780040703.01.T01:CDS
TGATGTAGTTTACGCGGAAG+TGG 0.516897 7:+81397463 None:intergenic
ATGGACGGCGCGTGGCGGAG+AGG 0.522247 7:+81397568 None:intergenic
GTGTTGGTTTCGGTTGTGGT+TGG 0.533999 7:-81397228 MsG0780040703.01.T01:CDS
GATGAACATGAAGGGTATGA+TGG 0.534737 7:-81397150 MsG0780040703.01.T01:CDS
GAGTACAGAGGATTTGTATG+TGG 0.534977 7:-81397172 MsG0780040703.01.T01:CDS
GGACGGCGCGTGGCGGAGAG+GGG 0.537542 7:+81397570 None:intergenic
TTCTTCCCATGGACGGCGCG+TGG 0.540922 7:+81397560 None:intergenic
GAGGAATGGAATTTGATGAT+GGG 0.542880 7:+81397625 None:intergenic
TGCAGGGTATACCTTAATAT+AGG 0.553653 7:-81397082 None:intergenic
GGGCGTGAGATGAAATTTGG+AGG 0.564375 7:+81397606 None:intergenic
TTCCCATGGACGGCGCGTGG+CGG 0.568324 7:+81397563 None:intergenic
GAACATGAAGGGTATGATGG+AGG 0.570438 7:-81397147 MsG0780040703.01.T01:CDS
GGTGCTTGCGGCGGTGACTG+TGG 0.576471 7:-81397283 MsG0780040703.01.T01:CDS
GTTACGCTTCACGCGCGAGG+TGG 0.594481 7:+81397491 None:intergenic
GAGAGAGTATGGACGTGTGA+AGG 0.602366 7:+81397521 None:intergenic
AGTGCGAAAACTTCTTCCCA+TGG 0.611042 7:+81397549 None:intergenic
GACTGAGGAATAAGACGAAG+AGG 0.612998 7:+81397433 None:intergenic
TTGTATGTGGATGAACATGA+AGG 0.615757 7:-81397159 MsG0780040703.01.T01:CDS
TGATTGAGATAGGATGCCAT+AGG 0.623237 7:+81397409 None:intergenic
CGAAAACTTCTTCCCATGGA+CGG 0.634534 7:+81397553 None:intergenic
TAGGATGCCATAGGAGACTG+AGG 0.641152 7:+81397418 None:intergenic
GATGTTACGCTTCACGCGCG+AGG 0.644346 7:+81397488 None:intergenic
CCCCTACAAAACGTGGTGCA+GGG 0.646335 7:-81397098 MsG0780040703.01.T01:CDS
ACAGAACAATCGGTTCGTCA+CGG 0.650504 7:+81397340 None:intergenic
AAGCTCTATCATCGACAACA+CGG 0.654426 7:+81397313 None:intergenic
GCTTGCGGCGGTGACTGTGG+TGG 0.673252 7:-81397280 MsG0780040703.01.T01:CDS
TTGCTTAATTCGTTTGTTGG+GGG 0.680040 7:-81397123 MsG0780040703.01.T01:CDS
GGGAGAGAACTGTAATGAGG+TGG 0.684799 7:+81397645 None:intergenic
TGTCGATGATAGAGCTTCTG+CGG 0.689430 7:-81397307 MsG0780040703.01.T01:CDS
GATGGGAGAGAACTGTAATG+AGG 0.691558 7:+81397642 None:intergenic
TGAGGATGATGTAGTTTACG+CGG 0.702002 7:+81397457 None:intergenic
CGATGATAGAGCTTCTGCGG+CGG 0.707409 7:-81397304 MsG0780040703.01.T01:CDS
TGGTGCGTTTAGGAGTACAG+AGG 0.718243 7:-81397184 MsG0780040703.01.T01:CDS
TGTATGTGGATGAACATGAA+GGG 0.731483 7:-81397158 MsG0780040703.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GATTGCTTAATTCGTTTGTT+GGG - Chr7:81397624-81397643 MsG0780040703.01.T01:CDS 30.0%
! ATTGCTTAATTCGTTTGTTG+GGG - Chr7:81397625-81397644 MsG0780040703.01.T01:CDS 30.0%
AAGAAGACGATGATTGAGAT+AGG + Chr7:81397353-81397372 None:intergenic 35.0%
GGATTGCTTAATTCGTTTGT+TGG - Chr7:81397623-81397642 MsG0780040703.01.T01:CDS 35.0%
TGAGATGAAATTTGGAGGAA+TGG + Chr7:81397141-81397160 None:intergenic 35.0%
TGTATGTGGATGAACATGAA+GGG - Chr7:81397591-81397610 MsG0780040703.01.T01:CDS 35.0%
TTGTATGTGGATGAACATGA+AGG - Chr7:81397590-81397609 MsG0780040703.01.T01:CDS 35.0%
! GAGGAATGGAATTTGATGAT+GGG + Chr7:81397127-81397146 None:intergenic 35.0%
! TTGCTTAATTCGTTTGTTGG+GGG - Chr7:81397626-81397645 MsG0780040703.01.T01:CDS 35.0%
!!! GTTAGCTTTTACTGGTGTTT+GGG - Chr7:81397493-81397512 MsG0780040703.01.T01:CDS 35.0%
AAGCTCTATCATCGACAACA+CGG + Chr7:81397439-81397458 None:intergenic 40.0%
GAGTACAGAGGATTTGTATG+TGG - Chr7:81397577-81397596 MsG0780040703.01.T01:CDS 40.0%
GGAGGAATGGAATTTGATGA+TGG + Chr7:81397128-81397147 None:intergenic 40.0%
TCTTATTCCTCAGTCTCCTA+TGG - Chr7:81397324-81397343 MsG0780040703.01.T01:CDS 40.0%
TCTTGATCACACTGATAGCT+TGG - Chr7:81397369-81397388 MsG0780040703.01.T01:CDS 40.0%
TGAGGATGATGTAGTTTACG+CGG + Chr7:81397295-81397314 None:intergenic 40.0%
TGATTGAGATAGGATGCCAT+AGG + Chr7:81397343-81397362 None:intergenic 40.0%
! GATGAACATGAAGGGTATGA+TGG - Chr7:81397599-81397618 MsG0780040703.01.T01:CDS 40.0%
!! TTGGGTGAATGTGTTGGTTT+CGG - Chr7:81397511-81397530 MsG0780040703.01.T01:CDS 40.0%
!!! CGTTAGCTTTTACTGGTGTT+TGG - Chr7:81397492-81397511 MsG0780040703.01.T01:CDS 40.0%
ACACGGCCAAACAGAACAAT+CGG + Chr7:81397422-81397441 None:intergenic 45.0%
AGTGCGAAAACTTCTTCCCA+TGG + Chr7:81397203-81397222 None:intergenic 45.0%
CGAAAACTTCTTCCCATGGA+CGG + Chr7:81397199-81397218 None:intergenic 45.0%
GACGAACCGATTGTTCTGTT+TGG - Chr7:81397413-81397432 MsG0780040703.01.T01:CDS 45.0%
GACTGAGGAATAAGACGAAG+AGG + Chr7:81397319-81397338 None:intergenic 45.0%
GATGGGAGAGAACTGTAATG+AGG + Chr7:81397110-81397129 None:intergenic 45.0%
GGAATAAGACGAAGAGGATG+AGG + Chr7:81397313-81397332 None:intergenic 45.0%
GTTGTGGTTGTTGTGTTGCA+TGG - Chr7:81397545-81397564 MsG0780040703.01.T01:CDS 45.0%
TGATGTAGTTTACGCGGAAG+TGG + Chr7:81397289-81397308 None:intergenic 45.0%
TGGTGTTTGGGTGAATGTGT+TGG - Chr7:81397505-81397524 MsG0780040703.01.T01:CDS 45.0%
TGTCGATGATAGAGCTTCTG+CGG - Chr7:81397442-81397461 MsG0780040703.01.T01:CDS 45.0%
TTGTGTTGCATGGTGCGTTT+AGG - Chr7:81397555-81397574 MsG0780040703.01.T01:CDS 45.0%
! GAACATGAAGGGTATGATGG+AGG - Chr7:81397602-81397621 MsG0780040703.01.T01:CDS 45.0%
!! ACAGAACAATCGGTTCGTCA+CGG + Chr7:81397412-81397431 None:intergenic 45.0%
!! GAATGTGTTGGTTTCGGTTG+TGG - Chr7:81397517-81397536 MsG0780040703.01.T01:CDS 45.0%
GGGAGAGAACTGTAATGAGG+TGG + Chr7:81397107-81397126 None:intergenic 50.0%
GGGCGTGAGATGAAATTTGG+AGG + Chr7:81397146-81397165 None:intergenic 50.0%
GTGGGGCGTGAGATGAAATT+TGG + Chr7:81397149-81397168 None:intergenic 50.0%
TAGGATGCCATAGGAGACTG+AGG + Chr7:81397334-81397353 None:intergenic 50.0%
! ACCCTGCACCACGTTTTGTA+GGG + Chr7:81397655-81397674 None:intergenic 50.0%
! GAGAGAGTATGGACGTGTGA+AGG + Chr7:81397231-81397250 None:intergenic 50.0%
! TACCCTGCACCACGTTTTGT+AGG + Chr7:81397656-81397675 None:intergenic 50.0%
! TGGTGCGTTTAGGAGTACAG+AGG - Chr7:81397565-81397584 MsG0780040703.01.T01:CDS 50.0%
!! GTGTTGGTTTCGGTTGTGGT+TGG - Chr7:81397521-81397540 MsG0780040703.01.T01:CDS 50.0%
!! TTCGGTTGTGGTTGGTGTTG+TGG - Chr7:81397529-81397548 MsG0780040703.01.T01:CDS 50.0%
!!! GTGGTGGCGTTAGCTTTTAC+TGG - Chr7:81397485-81397504 MsG0780040703.01.T01:CDS 50.0%
ACCCCTACAAAACGTGGTGC+AGG - Chr7:81397650-81397669 MsG0780040703.01.T01:CDS 55.0%
CCCCTACAAAACGTGGTGCA+GGG - Chr7:81397651-81397670 MsG0780040703.01.T01:CDS 55.0%
CGATGATAGAGCTTCTGCGG+CGG - Chr7:81397445-81397464 MsG0780040703.01.T01:CDS 55.0%
GTGGCTTCGGTGAGAGAGTA+TGG + Chr7:81397242-81397261 None:intergenic 55.0%
! CCCTGCACCACGTTTTGTAG+GGG + Chr7:81397654-81397673 None:intergenic 55.0%
!! AGAGGGGTTTTGGTGTGGTG+GGG + Chr7:81397166-81397185 None:intergenic 55.0%
!! GAGAGGGGTTTTGGTGTGGT+GGG + Chr7:81397167-81397186 None:intergenic 55.0%
GATGTTACGCTTCACGCGCG+AGG + Chr7:81397264-81397283 None:intergenic 60.0%
!! GGAGAGGGGTTTTGGTGTGG+TGG + Chr7:81397168-81397187 None:intergenic 60.0%
!! GGGGGTACCCCTACAAAACG+TGG - Chr7:81397644-81397663 MsG0780040703.01.T01:CDS 60.0%
AGCTTCTGCGGCGGTGCTTG+CGG - Chr7:81397454-81397473 MsG0780040703.01.T01:CDS 65.0%
CTTCACGCGCGAGGTGGCTT+CGG + Chr7:81397255-81397274 None:intergenic 65.0%
GTTACGCTTCACGCGCGAGG+TGG + Chr7:81397261-81397280 None:intergenic 65.0%
TTCTTCCCATGGACGGCGCG+TGG + Chr7:81397192-81397211 None:intergenic 65.0%
!! GGCGGAGAGGGGTTTTGGTG+TGG + Chr7:81397171-81397190 None:intergenic 65.0%
GCTTGCGGCGGTGACTGTGG+TGG - Chr7:81397469-81397488 MsG0780040703.01.T01:CDS 70.0%
GGTGCTTGCGGCGGTGACTG+TGG - Chr7:81397466-81397485 MsG0780040703.01.T01:CDS 70.0%
TTCCCATGGACGGCGCGTGG+CGG + Chr7:81397189-81397208 None:intergenic 70.0%
TTCTGCGGCGGTGCTTGCGG+CGG - Chr7:81397457-81397476 MsG0780040703.01.T01:CDS 70.0%
!! CGCGTGGCGGAGAGGGGTTT+TGG + Chr7:81397176-81397195 None:intergenic 70.0%
CTCCGCCACGCGCCGTCCAT+GGG - Chr7:81397184-81397203 MsG0780040703.01.T01:CDS 75.0%
TCTCCGCCACGCGCCGTCCA+TGG - Chr7:81397183-81397202 MsG0780040703.01.T01:CDS 75.0%
! ATGGACGGCGCGTGGCGGAG+AGG + Chr7:81397184-81397203 None:intergenic 75.0%
! TGGACGGCGCGTGGCGGAGA+GGG + Chr7:81397183-81397202 None:intergenic 75.0%
!! GGACGGCGCGTGGCGGAGAG+GGG + Chr7:81397182-81397201 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr7 gene 81397083 81397688 81397083 ID=MsG0780040703.01;Name=MsG0780040703.01
Chr7 mRNA 81397083 81397688 81397083 ID=MsG0780040703.01.T01;Parent=MsG0780040703.01;Name=MsG0780040703.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|201
Chr7 exon 81397083 81397688 81397083 ID=MsG0780040703.01.T01:exon:17730;Parent=MsG0780040703.01.T01
Chr7 CDS 81397083 81397688 81397083 ID=MsG0780040703.01.T01:cds;Parent=MsG0780040703.01.T01
Gene Sequence

>MsG0780040703.01.T01

ATGAATTCTTCATCTTCTTCACCACCTCATTACAGTTCTCTCCCATCATCAAATTCCATTCCTCCAAATTTCATCTCACGCCCCACCACACCAAAACCCCTCTCCGCCACGCGCCGTCCATGGGAAGAAGTTTTCGCACTCTACTCCTTCACACGTCCATACTCTCTCACCGAAGCCACCTCGCGCGTGAAGCGTAACATCAACCACTTCCGCGTAAACTACATCATCCTCATCCTCTTCGTCTTATTCCTCAGTCTCCTATGGCATCCTATCTCAATCATCGTCTTCTTGATCACACTGATAGCTTGGTTTTTTCTCTACTTCTTCCGTGACGAACCGATTGTTCTGTTTGGCCGTGTTGTCGATGATAGAGCTTCTGCGGCGGTGCTTGCGGCGGTGACTGTGGTGGCGTTAGCTTTTACTGGTGTTTGGGTGAATGTGTTGGTTTCGGTTGTGGTTGGTGTTGTGGTTGTTGTGTTGCATGGTGCGTTTAGGAGTACAGAGGATTTGTATGTGGATGAACATGAAGGGTATGATGGAGGATTGCTTAATTCGTTTGTTGGGGGTACCCCTACAAAACGTGGTGCAGGGTATACCTTAATATAG

Protein sequence

>MsG0780040703.01.T01

MNSSSSSPPHYSSLPSSNSIPPNFISRPTTPKPLSATRRPWEEVFALYSFTRPYSLTEATSRVKRNINHFRVNYIILILFVLFLSLLWHPISIIVFLITLIAWFFLYFFRDEPIVLFGRVVDDRASAAVLAAVTVVALAFTGVWVNVLVSVVVGVVVVVLHGAFRSTEDLYVDEHEGYDGGLLNSFVGGTPTKRGAGYTLI*