AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040869.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040869.01.T01 MTR_7g100320 81.111 180 13 2 2 181 48 206 5.39e-97 283
MsG0780040869.01.T01 MTR_1g067180 68.132 182 37 2 2 183 49 209 2.02e-83 248
MsG0780040869.01.T01 MTR_1g067180 68.333 180 36 2 2 181 49 207 2.15e-82 246
MsG0780040869.01.T01 MTR_1g067180 68.333 180 36 2 2 181 46 204 3.01e-82 246
MsG0780040869.01.T01 MTR_1g067180 71.538 130 33 1 2 131 49 174 2.99e-61 191
MsG0780040869.01.T01 MTR_1g067180 72.441 127 31 1 2 128 49 171 4.12e-61 190
MsG0780040869.01.T01 MTR_1g067170 56.906 181 29 4 2 181 46 178 1.15e-57 184
MsG0780040869.01.T01 MTR_1g116270 54.444 180 60 3 2 181 123 280 1.60e-57 185
MsG0780040869.01.T01 MTR_1g067150 55.556 180 32 3 2 181 44 175 2.65e-54 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040869.01.T01 AT5G02790 60.000 180 51 2 2 181 50 208 1.18e-70 216
MsG0780040869.01.T01 AT5G02780 57.143 182 56 3 1 181 51 211 5.09e-67 207
MsG0780040869.01.T01 AT5G02780 57.143 182 56 3 1 181 51 211 5.09e-67 207
MsG0780040869.01.T01 AT5G02780 57.143 182 54 4 1 181 51 209 6.31e-65 202
MsG0780040869.01.T01 AT3G55040 51.111 180 67 2 2 181 102 260 3.52e-57 184

Find 58 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACAGGCCTGTTTGGTATA+AGG 0.282787 7:-83674785 MsG0780040869.01.T01:CDS
TCACCAAACTCTTTCTTTGC+AGG 0.295908 7:+83674481 None:intergenic
TCCGTTTCCATTTGCGGAAC+AGG 0.303576 7:+83673762 None:intergenic
CCCATTTCCGTTTCCATTTG+CGG 0.305037 7:+83673756 None:intergenic
CAATGAAGAGTACAGATCTT+TGG 0.308227 7:+83674429 None:intergenic
CGGAAATGGGCCACAGAAAA+TGG 0.312516 7:-83673743 MsG0780040869.01.T01:CDS
ACTACAAGACAAAATCAAAT+TGG 0.371760 7:-83674824 MsG0780040869.01.T01:CDS
AGGTCCTGCCTTTGATTACT+TGG 0.371880 7:-83674296 MsG0780040869.01.T01:CDS
ACACCTCAGAAAAGACTATA+TGG 0.374447 7:+83673941 None:intergenic
TCAATCCATGCTGCAAGTTT+CGG 0.375231 7:+83673892 None:intergenic
TCCGCAAATGGAAACGGAAA+TGG 0.375924 7:-83673757 MsG0780040869.01.T01:CDS
TCTTTCAACAAATGGAATAT+AGG 0.379083 7:+83673966 None:intergenic
ACCTTCAAGACAGGCCTGTT+TGG 0.380904 7:-83674792 MsG0780040869.01.T01:CDS
GGCCTGTCTTGAAGGTTAAT+AGG 0.408791 7:+83674799 None:intergenic
ATATGGAATCTTTCAACAAA+TGG 0.410892 7:+83673958 None:intergenic
ACCAAACAGGCCTGTCTTGA+AGG 0.413697 7:+83674791 None:intergenic
AACTTAACCTGTTCCGCAAA+TGG 0.414010 7:-83673769 MsG0780040869.01.T01:CDS
TATGAATTGATTACAAATCA+AGG 0.418475 7:-83673814 MsG0780040869.01.T01:CDS
TTCAAGCATGATATAATAGA+AGG 0.420899 7:-83673919 MsG0780040869.01.T01:CDS
GATCCTGCAAAGAAAGAGTT+TGG 0.421571 7:-83674484 MsG0780040869.01.T01:CDS
GGAGCACAATGGGAAGGTCT+TGG 0.435080 7:-83674649 MsG0780040869.01.T01:CDS
ACTTTCTCCTTATACCAAAC+AGG 0.438140 7:+83674778 None:intergenic
TTCATAAACGAATGCAATTG+TGG 0.455775 7:+83673831 None:intergenic
GATTACTTGGAGAATGCTCT+TGG 0.461042 7:-83674283 MsG0780040869.01.T01:CDS
GAGCACAATGGGAAGGTCTT+GGG 0.461051 7:-83674648 MsG0780040869.01.T01:CDS
TGCCATCGTTGGAGCACAAT+GGG 0.465276 7:-83674659 MsG0780040869.01.T01:CDS
TACATAGATGTCAACTTTGA+GGG 0.472144 7:-83674603 MsG0780040869.01.T01:CDS
TTGATGATGGACAATTTCCT+AGG 0.478787 7:-83674255 MsG0780040869.01.T01:CDS
TTACATAGATGTCAACTTTG+AGG 0.479491 7:-83674604 MsG0780040869.01.T01:CDS
ACCTGTTCCGCAAATGGAAA+CGG 0.480344 7:-83673763 MsG0780040869.01.T01:CDS
CCGCAAATGGAAACGGAAAT+GGG 0.488839 7:-83673756 MsG0780040869.01.T01:CDS
TTGTCTTGTAGTCCCTGCAT+TGG 0.490449 7:+83674835 None:intergenic
GCTCTTGGTAAATTTGATGA+TGG 0.492656 7:-83674268 MsG0780040869.01.T01:CDS
TTACCCAACTGAATTGTCCT+AGG 0.504543 7:+83674238 None:intergenic
ATTTCCTAGGACAATTCAGT+TGG 0.506498 7:-83674242 MsG0780040869.01.T01:CDS
TTCCTATTAACCTTCAAGAC+AGG 0.513215 7:-83674801 MsG0780040869.01.T01:CDS
TCATAAACGAATGCAATTGT+GGG 0.515423 7:+83673832 None:intergenic
TAACGAGACATCGGAGGTAA+TGG 0.516290 7:+83673694 None:intergenic
GATCTGTACTCTTCATTGAA+AGG 0.526123 7:-83674424 MsG0780040869.01.T01:CDS
TTTCCTAGGACAATTCAGTT+GGG 0.539300 7:-83674241 MsG0780040869.01.T01:CDS
TTCTCCAAGTAATCAAAGGC+AGG 0.548201 7:+83674292 None:intergenic
TTCCCATTGTGCTCCAACGA+TGG 0.562767 7:+83674657 None:intergenic
CATGATTGATGACCAATGCA+GGG 0.569230 7:-83674847 None:intergenic
GGAGATCCTATAAAACAAGC+TGG 0.576421 7:-83674403 MsG0780040869.01.T01:intron
CTCACACTGGGAACTAGAGA+TGG 0.581337 7:+83674579 None:intergenic
AGAACTCTTGTCGAAGCTTG+AGG 0.581798 7:+83673719 None:intergenic
GAAACTTGCAGCATGGATTG+AGG 0.586391 7:-83673890 MsG0780040869.01.T01:CDS
AGCATTCTCCAAGTAATCAA+AGG 0.590018 7:+83674288 None:intergenic
AGGTCATCAATCCGAACTTG+AGG 0.592258 7:-83673794 MsG0780040869.01.T01:CDS
GAAAGTCTACCCAGAAAACA+AGG 0.594976 7:-83674761 MsG0780040869.01.T01:intron
GTATGTATAGGTGCCATCGT+TGG 0.598903 7:-83674670 MsG0780040869.01.T01:intron
GTGCCATCGTTGGAGCACAA+TGG 0.601381 7:-83674660 MsG0780040869.01.T01:CDS
AGCATGATATAATAGAAGGA+AGG 0.603963 7:-83673915 MsG0780040869.01.T01:CDS
ATCGTTGGAGCACAATGGGA+AGG 0.614660 7:-83674655 MsG0780040869.01.T01:CDS
ACATAGATGTCAACTTTGAG+GGG 0.620984 7:-83674602 MsG0780040869.01.T01:CDS
GAAGGCCGAAACTTGCAGCA+TGG 0.634072 7:-83673897 MsG0780040869.01.T01:CDS
ATCTCGTGTTAACGAGACAT+CGG 0.652124 7:+83673685 None:intergenic
TCGTGTTAACGAGACATCGG+AGG 0.672788 7:+83673688 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATAATCGATTTTTTTT+GGG + Chr7:83673990-83674009 None:intergenic 10.0%
!! AAAAAAATCGATTATATTAG+TGG - Chr7:83673991-83674010 MsG0780040869.01.T01:intron 15.0%
!! TCTTATATATAAGTTGTTAT+TGG - Chr7:83674513-83674532 MsG0780040869.01.T01:intron 15.0%
!!! AATATAATCGATTTTTTTTG+GGG + Chr7:83673989-83674008 None:intergenic 15.0%
!!! ATTATTTTTTTCGTATGTAT+AGG - Chr7:83673843-83673862 MsG0780040869.01.T01:CDS 15.0%
!!! ATTTTATGATTTTGTTTTTC+AGG - Chr7:83674019-83674038 MsG0780040869.01.T01:intron 15.0%
!!! CTAATATAATCGATTTTTTT+TGG + Chr7:83673991-83674010 None:intergenic 15.0%
!! GAAAAAAATAATGATCAACT+AGG + Chr7:83673835-83673854 None:intergenic 20.0%
!! TATGAATTGATTACAAATCA+AGG - Chr7:83674711-83674730 MsG0780040869.01.T01:intron 20.0%
!!! ATATAATCGATTTTTTTTGG+GGG + Chr7:83673988-83674007 None:intergenic 20.0%
!!! ATTTTAAGCATATTACTTGT+CGG - Chr7:83674353-83674372 MsG0780040869.01.T01:intron 20.0%
!!! ATTTTTAATAAAGCATGTGA+TGG - Chr7:83674396-83674415 MsG0780040869.01.T01:intron 20.0%
! ACTACAAGACAAAATCAAAT+TGG - Chr7:83673701-83673720 MsG0780040869.01.T01:CDS 25.0%
! ATATGGAATCTTTCAACAAA+TGG + Chr7:83674570-83674589 None:intergenic 25.0%
! TAAGAGAAAAACTATAGTCA+TGG + Chr7:83674498-83674517 None:intergenic 25.0%
! TCTTTCAACAAATGGAATAT+AGG + Chr7:83674562-83674581 None:intergenic 25.0%
! TTCAAGCATGATATAATAGA+AGG - Chr7:83674606-83674625 MsG0780040869.01.T01:CDS 25.0%
!! TGAAATTGGTAATTTGAATC+AGG - Chr7:83674209-83674228 MsG0780040869.01.T01:CDS 25.0%
!!! GCTTTCAAGTTAATAAATAG+AGG - Chr7:83674149-83674168 MsG0780040869.01.T01:intron 25.0%
AGCATGATATAATAGAAGGA+AGG - Chr7:83674610-83674629 MsG0780040869.01.T01:CDS 30.0%
CAACCAACAGAAAAGAAAAA+TGG + Chr7:83674332-83674351 None:intergenic 30.0%
TACATAGATGTCAACTTTGA+GGG - Chr7:83673922-83673941 MsG0780040869.01.T01:CDS 30.0%
TCATAAACGAATGCAATTGT+GGG + Chr7:83674696-83674715 None:intergenic 30.0%
TTACATAGATGTCAACTTTG+AGG - Chr7:83673921-83673940 MsG0780040869.01.T01:CDS 30.0%
TTCATAAACGAATGCAATTG+TGG + Chr7:83674697-83674716 None:intergenic 30.0%
! ATTCCATATAGTCTTTTCTG+AGG - Chr7:83674581-83674600 MsG0780040869.01.T01:intron 30.0%
!! TGTTATTGGTTGTGTTGATT+AGG - Chr7:83674527-83674546 MsG0780040869.01.T01:intron 30.0%
!!! GCAAACTGAACTTTTGAAAT+TGG - Chr7:83674195-83674214 MsG0780040869.01.T01:CDS 30.0%
!!! TTAAACACACCTTGTTTTCT+GGG + Chr7:83673776-83673795 None:intergenic 30.0%
!!! TTGCCATTTTTCTTTTCTGT+TGG - Chr7:83674326-83674345 MsG0780040869.01.T01:intron 30.0%
ACACCTCAGAAAAGACTATA+TGG + Chr7:83674587-83674606 None:intergenic 35.0%
ACATAGATGTCAACTTTGAG+GGG - Chr7:83673923-83673942 MsG0780040869.01.T01:CDS 35.0%
ACTTTCTCCTTATACCAAAC+AGG + Chr7:83673750-83673769 None:intergenic 35.0%
AGCATTCTCCAAGTAATCAA+AGG + Chr7:83674240-83674259 None:intergenic 35.0%
ATTTCCTAGGACAATTCAGT+TGG - Chr7:83674283-83674302 MsG0780040869.01.T01:CDS 35.0%
GATCTGTACTCTTCATTGAA+AGG - Chr7:83674101-83674120 MsG0780040869.01.T01:intron 35.0%
GCTCTTGGTAAATTTGATGA+TGG - Chr7:83674257-83674276 MsG0780040869.01.T01:CDS 35.0%
TTCCTATTAACCTTCAAGAC+AGG - Chr7:83673724-83673743 MsG0780040869.01.T01:CDS 35.0%
TTTCCTAGGACAATTCAGTT+GGG - Chr7:83674284-83674303 MsG0780040869.01.T01:CDS 35.0%
! CAATGAAGAGTACAGATCTT+TGG + Chr7:83674099-83674118 None:intergenic 35.0%
! GGTTAAGTTTTCCTCAAGTT+CGG + Chr7:83674745-83674764 None:intergenic 35.0%
!! GTGTTTAACTTGCTCATACA+GGG - Chr7:83673788-83673807 MsG0780040869.01.T01:CDS 35.0%
!! TATTGGTTGTGTTGATTAGG+TGG - Chr7:83674530-83674549 MsG0780040869.01.T01:intron 35.0%
!! TGTGTTTAACTTGCTCATAC+AGG - Chr7:83673787-83673806 MsG0780040869.01.T01:CDS 35.0%
!! TTGATGATGGACAATTTCCT+AGG - Chr7:83674270-83674289 MsG0780040869.01.T01:CDS 35.0%
!!! GCATTACCAGCTTGTTTTAT+AGG + Chr7:83674131-83674150 None:intergenic 35.0%
!!! GTTAAACACACCTTGTTTTC+TGG + Chr7:83673777-83673796 None:intergenic 35.0%
AACTTAACCTGTTCCGCAAA+TGG - Chr7:83674756-83674775 MsG0780040869.01.T01:intron 40.0%
GAAAGTCTACCCAGAAAACA+AGG - Chr7:83673764-83673783 MsG0780040869.01.T01:CDS 40.0%
GATCCTGCAAAGAAAGAGTT+TGG - Chr7:83674041-83674060 MsG0780040869.01.T01:intron 40.0%
GATTACTTGGAGAATGCTCT+TGG - Chr7:83674242-83674261 MsG0780040869.01.T01:CDS 40.0%
GGAGATCCTATAAAACAAGC+TGG - Chr7:83674122-83674141 MsG0780040869.01.T01:intron 40.0%
TAAAGCATGTGATGGTCTGA+TGG - Chr7:83674404-83674423 MsG0780040869.01.T01:CDS 40.0%
TCAATCCATGCTGCAAGTTT+CGG + Chr7:83674636-83674655 None:intergenic 40.0%
TCACCAAACTCTTTCTTTGC+AGG + Chr7:83674047-83674066 None:intergenic 40.0%
TTACCCAACTGAATTGTCCT+AGG + Chr7:83674290-83674309 None:intergenic 40.0%
TTCTCCAAGTAATCAAAGGC+AGG + Chr7:83674236-83674255 None:intergenic 40.0%
ACCTGTTCCGCAAATGGAAA+CGG - Chr7:83674762-83674781 MsG0780040869.01.T01:CDS 45.0%
AGAACTCTTGTCGAAGCTTG+AGG + Chr7:83674809-83674828 None:intergenic 45.0%
AGACAGGCCTGTTTGGTATA+AGG - Chr7:83673740-83673759 MsG0780040869.01.T01:CDS 45.0%
AGGTCATCAATCCGAACTTG+AGG - Chr7:83674731-83674750 MsG0780040869.01.T01:intron 45.0%
AGGTCCTGCCTTTGATTACT+TGG - Chr7:83674229-83674248 MsG0780040869.01.T01:CDS 45.0%
CCCATTTCCGTTTCCATTTG+CGG + Chr7:83674772-83674791 None:intergenic 45.0%
CCGCAAATGGAAACGGAAAT+GGG - Chr7:83674769-83674788 MsG0780040869.01.T01:CDS 45.0%
GGCCTGTCTTGAAGGTTAAT+AGG + Chr7:83673729-83673748 None:intergenic 45.0%
TAACGAGACATCGGAGGTAA+TGG + Chr7:83674834-83674853 None:intergenic 45.0%
TCCGCAAATGGAAACGGAAA+TGG - Chr7:83674768-83674787 MsG0780040869.01.T01:CDS 45.0%
! AGCTTGAGGACCATTTTCTG+TGG + Chr7:83674795-83674814 None:intergenic 45.0%
! GAAACTTGCAGCATGGATTG+AGG - Chr7:83674635-83674654 MsG0780040869.01.T01:CDS 45.0%
! GTATGTATAGGTGCCATCGT+TGG - Chr7:83673855-83673874 MsG0780040869.01.T01:CDS 45.0%
!!! TCGATTTTTTTTGGGGGTGC+AGG + Chr7:83673982-83674001 None:intergenic 45.0%
ACCAAACAGGCCTGTCTTGA+AGG + Chr7:83673737-83673756 None:intergenic 50.0%
ACCTTCAAGACAGGCCTGTT+TGG - Chr7:83673733-83673752 MsG0780040869.01.T01:CDS 50.0%
ATCGTTGGAGCACAATGGGA+AGG - Chr7:83673870-83673889 MsG0780040869.01.T01:CDS 50.0%
CGGAAATGGGCCACAGAAAA+TGG - Chr7:83674782-83674801 MsG0780040869.01.T01:CDS 50.0%
CTCACACTGGGAACTAGAGA+TGG + Chr7:83673949-83673968 None:intergenic 50.0%
GAGCACAATGGGAAGGTCTT+GGG - Chr7:83673877-83673896 MsG0780040869.01.T01:CDS 50.0%
TCCGTTTCCATTTGCGGAAC+AGG + Chr7:83674766-83674785 None:intergenic 50.0%
TGCCATCGTTGGAGCACAAT+GGG - Chr7:83673866-83673885 MsG0780040869.01.T01:CDS 50.0%
TTCCCATTGTGCTCCAACGA+TGG + Chr7:83673871-83673890 None:intergenic 50.0%
! TGTGATGGTCTGATGGATCG+TGG - Chr7:83674411-83674430 MsG0780040869.01.T01:CDS 50.0%
!! AGGAGAGTGCTTTCTCACAC+TGG + Chr7:83673962-83673981 None:intergenic 50.0%
!! GGAGAGTGCTTTCTCACACT+GGG + Chr7:83673961-83673980 None:intergenic 50.0%
GAAGGCCGAAACTTGCAGCA+TGG - Chr7:83674628-83674647 MsG0780040869.01.T01:CDS 55.0%
GGAGCACAATGGGAAGGTCT+TGG - Chr7:83673876-83673895 MsG0780040869.01.T01:CDS 55.0%
GTGCCATCGTTGGAGCACAA+TGG - Chr7:83673865-83673884 MsG0780040869.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 83673693 83674854 83673693 ID=MsG0780040869.01;Name=MsG0780040869.01
Chr7 mRNA 83673693 83674854 83673693 ID=MsG0780040869.01.T01;Parent=MsG0780040869.01;Name=MsG0780040869.01.T01;_AED=0.35;_eAED=0.52;_QI=0|0.75|0.4|1|1|1|5|0|246
Chr7 exon 83674762 83674854 83674762 ID=MsG0780040869.01.T01:exon:19838;Parent=MsG0780040869.01.T01
Chr7 exon 83674584 83674682 83674584 ID=MsG0780040869.01.T01:exon:19837;Parent=MsG0780040869.01.T01
Chr7 exon 83674404 83674506 83674404 ID=MsG0780040869.01.T01:exon:19836;Parent=MsG0780040869.01.T01
Chr7 exon 83674177 83674316 83674177 ID=MsG0780040869.01.T01:exon:19835;Parent=MsG0780040869.01.T01
Chr7 exon 83673693 83673998 83673693 ID=MsG0780040869.01.T01:exon:19834;Parent=MsG0780040869.01.T01
Chr7 CDS 83674762 83674854 83674762 ID=MsG0780040869.01.T01:cds;Parent=MsG0780040869.01.T01
Chr7 CDS 83674584 83674682 83674584 ID=MsG0780040869.01.T01:cds;Parent=MsG0780040869.01.T01
Chr7 CDS 83674404 83674506 83674404 ID=MsG0780040869.01.T01:cds;Parent=MsG0780040869.01.T01
Chr7 CDS 83674177 83674316 83674177 ID=MsG0780040869.01.T01:cds;Parent=MsG0780040869.01.T01
Chr7 CDS 83673693 83673998 83673693 ID=MsG0780040869.01.T01:cds;Parent=MsG0780040869.01.T01
Gene Sequence

>MsG0780040869.01.T01

ATGCAGGGACTACAAGACAAAATCAAATTGGTTCCTATTAACCTTCAAGACAGGCCTGTTTGGTATAAGGAGAAAGTCTACCCAGAAAACAAGGTGCCATCGTTGGAGCACAATGGGAAGGTCTTGGGAGAAAGTCTCGATTTGATCAGTTACATAGATGTCAACTTTGAGGGGCCATCTCTAGTTCCCAGTGATCCTGCAAAGAAAGAGTTTGGTGATCAGTTGATTTCTCATGTTGATACATTCACCAAAGATCTGTACTCTTCATTGAAAGGAGATCCTATAAAACAAGCTGGTCCTGCCTTTGATTACTTGGAGAATGCTCTTGGTAAATTTGATGATGGACAATTTCCTAGGACAATTCAGTTGGGTAAGTTGAAATTAAGAGACTTGCCATTTTTCTTTTCTGTTGGTTGCATTTTAAGCATATTACTTGTGGATTTTGCCTATATTCCATTTGTTGAAAGATTCCATATAGTCTTTTCTGAGGTGTTCAAGCATGATATAATAGAAGGAAGGCCGAAACTTGCAGCATGGATTGAGGTACAGTTGAAACAGTTCTCTCTAAAATTGCAGTTTCCCACAATTGCATTCGTTTATGAATTGATTACAAATCAAGGTCATCAATCCGAACTTGAGGAAAACTTAACCTGTTCCGCAAATGGAAACGGAAATGGGCCACAGAAAATGGTCCTCAAGCTTCGACAAGAGTTCTTACCATTACCTCCGATGTCTCGTTAA

Protein sequence

>MsG0780040869.01.T01

MQGLQDKIKLVPINLQDRPVWYKEKVYPENKVPSLEHNGKVLGESLDLISYIDVNFEGPSLVPSDPAKKEFGDQLISHVDTFTKDLYSSLKGDPIKQAGPAFDYLENALGKFDDGQFPRTIQLGKLKLRDLPFFFSVGCILSILLVDFAYIPFVERFHIVFSEVFKHDIIEGRPKLAAWIEVQLKQFSLKLQFPTIAFVYELITNQGHQSELEENLTCSANGNGNGPQKMVLKLRQEFLPLPPMSR*